Disclosed is a fluorescently labeled peptide library useful for diagnosing multiple sclerosis. The fluorescently labeled peptide library comprising fluorescently labeled peptides represented by the following formula:
Disclosed is a fluorescently labeled peptide library useful for diagnosing multiple sclerosis. The fluorescently labeled peptide library comprising fluorescently labeled peptides represented by the following formula:
wherein X1, X2, X3 and X4 are independently arbitrary amino acid residues, and the peptides are immobilized on a chip.
C40B 40/10 - Bibliothèques comprenant des peptides ou des polypeptides ou leurs dérivés
C07K 14/00 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés
C07K 17/00 - Peptides fixés sur un support ou immobilisésLeur préparation
C40B 20/04 - Identification des éléments d'une bibliothèque au moyen d'une étiquette, d'un marqueur ou d'un autre identificateur lisible ou détectable, p. ex. procédés de décodage
C40B 70/00 - Étiquettes ["tags"] ou marqueurs ["labels"] spécialement adaptés à la chimie combinatoire ou aux chimiothèques, p. ex. "tags" fluorescents ou codes-barres
Disclosed is a peptide-immobilized bead library from which peptides are more likely to be obtained as candidates of useful substances such as pharmaceuticals, and with which the amino acid sequence bound to a target substance can be more easily analyzed, compared to known OPOB libraries. The peptide-immobilized bead library has a structure of General Formula [I].
Disclosed is a peptide-immobilized bead library from which peptides are more likely to be obtained as candidates of useful substances such as pharmaceuticals, and with which the amino acid sequence bound to a target substance can be more easily analyzed, compared to known OPOB libraries. The peptide-immobilized bead library has a structure of General Formula [I].
Disclosed is a peptide-immobilized bead library from which peptides are more likely to be obtained as candidates of useful substances such as pharmaceuticals, and with which the amino acid sequence bound to a target substance can be more easily analyzed, compared to known OPOB libraries. The peptide-immobilized bead library has a structure of General Formula [I].
In General Formula [I], X is an amino acid residue; n is an integer of 4 to 8; Y is a cyclization structure; A is a spacer structure; B is a specific cleavage site-containing structure; and the 4 to 8 amino acid residues of X are each independently randomly selected.
Disclosed is a peptide-immobilized bead library which, in comparison to well-known OPOB libraries, has a higher potential for an obtained peptide to become a useful substance candidate for a drug or the like, and makes it easier to analyze an amino acid sequence bonded to a target substance. The peptide-immobilized bead library has a structure represented by formula [I]. In general formula [I], X represents an amino acid residue, n represents an integer of 4-8, Y represents a cyclic structure, A represents a spacer structure, B represents a specific cleavage site-containing structure, and the 4-8 amino acid residues represented by X are randomly selected independently of each other.
A substrate for biochips which has a high probe loading amounts and a uniform immobilization density, and which further has a high detection sensitivity and a high reproducibility by preventing a non-specific adsorption of proteins, when used as a substrate for biochips for immobilizing probes composed of biologically relevant substances such as proteins and nucleic acids, is disclosed. Amino groups can be bound to the surface of the substrate uniformly, at a high density and stably by covalently immobilizing an amino group-containing polymer on the surface of the substrate. The probe immobilization rate is high and immobilizing density was uniform by immobilizing a probe composed of a biologically relevant substance such as a protein or nucleic acid by utilizing the amino groups. Further, detection sensitivity and reproducibility are high by inhibiting non-specific adsorption of proteins.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
C08J 3/28 - Traitement par ondes énergétiques ou par rayonnement de particules
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
7.
Biochip substratum and method for production thereof
A substrate for biochips which has a high probe loading amounts and a uniform immobilization density, and which further has a high detection sensitivity and a high reproducibility by preventing a non-specific adsorption of proteins, when used as a substrate for biochips for immobilizing probes composed of biologically relevant substances such as proteins and nucleic acids, is disclosed. Amino groups can be bound to the surface of the substrate uniformly, at a high density and stably by covalently immobilizing an amino group-containing polymer on the surface of the substrate. The probe immobilization rate is high and immobilizing density was uniform by immobilizing a probe composed of a biologically relevant substance such as a protein or nucleic acid by utilizing the amino groups. Further, detection sensitivity and reproducibility are high by inhibiting non-specific adsorption of proteins.
Disclosed is an antioxidant agent which can exhibit a higher antioxidative ability than those of existing antioxidant peptides. The antioxidant agent comprises mimosine or a mimosine-containing peptide. The mimosine-containing peptide is a peptide which contains at least one mimosine residue in the amino acid sequence thereof. Mimosine has a higher antioxidative activity than those of known antioxidant tripeptides when used singly, and is therefore useful as an antioxidant agent. The antioxidative activity of the peptide can be increased by introducing mimosine to the peptide, and a peptide having a higher antioxidative activity than that of the original form thereof can be provided.
A61K 31/4412 - Pyridines non condenséesLeurs dérivés hydrogénés ayant des groupes oxo liés directement à l'hétérocycle
A61K 8/49 - Cosmétiques ou préparations similaires pour la toilette caractérisés par la composition contenant des composés organiques contenant des composés hétérocycliques
A61K 8/64 - ProtéinesPeptidesLeurs dérivés ou produits de dégradation
A61K 36/00 - Préparations médicinales de constitution indéterminée contenant du matériel provenant d'algues, de lichens, de champignons, ou de plantes, ou leurs dérivés, p. ex. médicaments traditionnels à base de plantes
A61K 38/00 - Préparations médicinales contenant des peptides
A61P 9/00 - Médicaments pour le traitement des troubles du système cardiovasculaire
A61P 17/00 - Médicaments pour le traitement des troubles dermatologiques
A substrate for biochips with which immobilization is easy, which does not exhibit self-fluorescence, which is easy to manufacture, and which is excellent in flatness and surface precision, is disclosed. A substrate having a substrate body of the biochip, which is made of a metal, and a carbon layer having functional groups formed on the metal substrate body is used as a substrate for biochips. Since the substrate body of the substrate for biochips is made of a metal, the substrate is not only easy to manufacture, but also free from cracking and chipping, so that it allows easy handling, and high flatness and surface precision can be attained. Therefore, the problem that the optical system is hard to focus when detecting fluorescence does not occur. Moreover, since the substrate body is made of a metal, it does not emit fluorescence by itself. In addition, since the carbon layer has functional groups such as amino groups, biologically relevant substances can be easily immobilized.
C40B 40/04 - Bibliothèques comprenant uniquement des composés organiques
C40B 50/14 - Synthèse en phase solide, c.-à-d. dans laquelle au moins un bloc servant à créer la bibliothèque est lié à un support solide au cours de la création de la bibliothèqueProcédés particuliers de clivage à partir du support solide
B32B 15/00 - Produits stratifiés composés essentiellement de métal
Means for overcoming the problems which the conventional glass microchannel chips have are disclosed. That is, a microchannel chip in which microchannels can be formed at a low cost, and which has a high chemical resistance is disclosed. The microchannel chip is constituted by a substrate made of carbon, which has a channel in its surface; and a cover composed of a glass plate bonded to the substrate. The cover is bonded to the substrate by heating at least a part of the contact surface at which the substrate is in contact with the cover.
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en généralAppareils appropriés
B01J 8/02 - Procédés chimiques ou physiques en général, conduits en présence de fluides et de particules solidesAppareillage pour de tels procédés avec des particules immobiles, p. ex. dans des lits fixes
B01J 35/02 - Catalyseurs caractérisés par leur forme ou leurs propriétés physiques, en général solides
B01J 8/00 - Procédés chimiques ou physiques en général, conduits en présence de fluides et de particules solidesAppareillage pour de tels procédés
B01J 10/00 - Procédés chimiques généraux faisant réagir un liquide avec des milieux gazeux autrement qu'en présence de particules solidesAppareillage spécialement adapté à cet effet
A biochip substrate which is free from cross-contamination due to spot spreading or contact with spots adjacent to each other, and a biochip using the same. A biochip substrate on which multiple valleys for immobilizing biological substances are formed so as to prevent cross-contamination due to spot spreading or contact with spots adjacent to each other, and a biochip using the same are provided. Moreover, it is found out that a desired binding in a target molecule contained in a test sample occurs at a detectable level in a solution system even in the case where a valley have such a small capacity as 1 nL to 10 nL.
C40B 60/14 - Appareils spécialement adaptés à une utilisation en chimie combinatoire ou avec des bibliothèques pour créer des bibliothèques
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B82Y 30/00 - Nanotechnologie pour matériaux ou science des surfaces, p. ex. nanocomposites
12.
BIOCHIP SUBSTRATUM AND METHOD FOR PRODUCTION THEREOF
Disclosed is a biochip substratum for immobilizing thereon a probe comprising a biological substance (e.g., a protein, a nucleic acid), wherein the biochip substratum can immobilize the probe at a high immobilization ratio and a uniform immobilization density, and can prevent the non-specific adsorption of a protein to thereby achieve high detection sensitivity and high reproducibility. A polymer containing an amino group is immobilized on the surface of a substratum via a covalent bond, thereby attaching the amino group to the surface of the substratum uniformly, at a high density and stably. By utilizing the amino group, a probe comprising a biological substance (e.g., a protein, a nucleic acid) can be immobilized. In this manner, it becomes possible to immobilize the probe at a high immobilization ratio and a uniform immobilization density, and to prevent the non-specific adsorption of a protein to thereby achieve high detection sensitivity and high reproducibility.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
Disclosed is means capable of solving the problem of the conventional micro-passage chip made of glass. This means is a micro-passage chip, which can be worked in its fine passage at a low cost and which has a high resistance to chemicals. The micro-passage chip comprises a substrate having a passage in its surface and made of carbon, and a cover made of a glass plate joined to the substrate. The cover is joined to the substrate by heating at least a portion of the contact face between the substrate and the cover.
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en généralAppareils appropriés
B81B 1/00 - Dispositifs sans éléments mobiles ou flexibles, p. ex. dispositifs capillaires microscopiques
C07C 1/32 - Préparation d'hydrocarbures à partir d'un ou plusieurs composés, aucun d'eux n'étant un hydrocarbure à partir de composés renfermant des hétéro-atomes autres que l'oxygène ou les halogènes, ou en addition à ceux-ci
C07C 15/12 - Hydrocarbures polycycliques non condensés
C07C 17/26 - Préparation d'hydrocarbures halogénés par des réactions comportant un accroissement du nombre des atomes de carbone dans le squelette
C07C 22/08 - Composés cycliques contenant des atomes d'halogène liés à un atome de carbone acyclique ayant une insaturation dans les cycles contenant des cycles aromatiques à six chaînons contenant du fluor
C07C 25/22 - Hydrocarbures halogénés aromatiques polycycliques à cycles condensés
C07C 41/30 - Préparation d'éthers par des réactions ne formant pas de liaisons sur l'oxygène de la fonction éther par augmentation du nombre d'atomes de carbone, p. ex. par oligomérisation
C07C 43/225 - Éthers une liaison sur l'oxygène de la fonction éther étant sur un atome de carbone d'un cycle aromatique à six chaînons contenant des atomes d'halogène
C40B 60/02 - Appareils intégrés spécialement adaptés à la création, au criblage de bibliothèques et à l'identification des éléments des bibliothèques
G01N 37/00 - Détails non couverts par les autres groupes de la présente sous-classe
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
brevets
