Abrégé

The present invention relates to genetically engineered micro-organisms for the combined production of 1,3-propanediol (PDO), 2,3-butanediol (BDO), and polyhydroxypropionic acid (PHP) by fermentation. In particular, the invention relates to a genetically engineered micro-organism suitable for combined production of PDO, BDO and PHP by fermentation, characterized in that: compared with corresponding wild-type starting micro-organism, the D-lactate dehydrogenase gene in the genetically engineered micro-organism is deleted or functionally inactivated, and the genetically engineered micro-organism comprises a heterogenous polynucleotide encoding the Coenzyme A-dependent Aldehyde dehydrogenase and a heterogenous polynucleotide encoding the Polyhydroxyalkanoate synthase. Methods for the construction of such micro-organisms, and methods for combined production of PDO, BDO and PHP by fermentation of a genetically engineered bacterium are also taught.

Classes IPC  ?

• C12P 7/42 - Acides hydroxycarboxyliques
• C12P 7/18 - Polyols
• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
• C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide

Abrégé

The present invention relates to genetically engineered micro-organisms for the combined production of 1,3-propanediol (PDO), 2,3-butanediol (BDO), and polyhydroxypropionic acid (PHP) by fermentation. In particular, the invention relates to a genetically engineered micro-organism suitable for combined production of PDO, BDO and PHP by fermentation, characterized in that: compared with corresponding wild-type starting micro-organism, the D-lactate dehydrogenase gene in the genetically engineered micro-organism is deleted or functionally inactivated, and the genetically engineered micro-organism comprises a heterogenous polynucleotide encoding the Coenzyme A-dependent Aldehyde dehydrogenase and a heterogenous polynucleotide encoding the Polyhydroxyalkanoate synthase. Methods for the construction of such micro-organisms, and methods for combined production of PDO, BDO and PHP by fermentation of a genetically engineered bacterium are also taught.

Classes IPC  ?

• C12P 7/42 - Acides hydroxycarboxyliques
• C12P 7/18 - Polyols
• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci

Abrégé

A method for co-production of 1,3-propanediol (PDO), 2,3-butanediol (BDO) and polyhydroxypropionic acid (PHP) via fermentation by genetic engineering bacteria comprises the following steps of: knocking out D type lactate dehydrogenase from wild PDO producing bacteria, introducing coenzyme A depending aldehyde dehydrogenase and polyhydroxy fatty acid synthase genes, constructing genetic engineering bacteria for producing PDO, BDO and PHP; fermenting aerobically and adjusting fermentation by adding mixture flow of glycerin and base solution; filtering fermentation broth by membrane, treating it by electrodialysis, concentrating, rectifying which separates PDO, BDO and PHP. The said genetic engineering bacteria can produce PDO, BDO and PHP at the same time, which increases utilization ratio of raw material, reduces cost, decreases generation of byproduct lactate, simplifies post extraction technique, lowers extraction cost, and increases generation of nicotinamide adenine dinucleotide 2 (NADH2) while PHP is introduced.

Classes IPC  ?

• C12P 7/18 - Polyols
• C12P 7/42 - Acides hydroxycarboxyliques
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
• C12R 1/22 - Klebsiella
• C12R 1/01 - Bactéries ou actinomycètes