Abrégé

The invention concerns novel streptavidin muteins and their use for determination, isolation or purification of proteins under denaturing conditons.In one embodiment such a mutein has an Cys residue at sequence position 127 of the wild-type sequence of streptavidin and comprises at least one mutation in the region of the amino acid positions 115 to 121 with reference to the amino acid sequence of wild type streptavidin.

Classes IPC  ?

• C07K 14/36 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant d'ActinomycesPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Streptomyces (G)
• C07K 17/00 - Peptides fixés sur un support ou immobilisésLeur préparation

Produits et services

(1) Chromatography chemicals; Chemical reagents for non-medical purposes, namely for use in separation and selection of cells; Reagents for use in scientific apparatus to isolate cells for chemical or biological analysis; Chemical reagents for chromatographic purification of biological materials; Chemical reagents for chromatographic purification of cells; Chemical chromatography materials loaded with streptavidin mutein for purification of cells. (2) Pharmaceuticals, human and veterinary preparations for research, diagnostics and treatment of cancer and other immune diseases, namely stem cells and immune cells; Medical diagnostic reagents. (3) Scientific apparatus for chromatographic purification of biological materials; Scientific apparatus for chromatographic purification of cells; Chromatography columns loaded with affinity chromatography reagents for purification of cells.

Produits et services

(1) Chemicals used in industry and science, namely muteins of streptavidin with improved binding properties to streptavidin binding peptides; chemicals used in industry and science, namely gene sequences of muteins of streptavidin with improved binding properties to streptavidin binding peptides; analysis kits used in science for purification of fusion proteins, mainly consisting of chemical reagents; analysis kits used in science for purification of fusion peptides, mainly consisting of chemical reagents; analysis kits used in science for detection of fusion proteins, mainly consisting of chemical reagents; analysis kits used in science for detection of fusion peptides, mainly consisting of chemical reagents; analysis kits used in science for immobilization of fusion proteins, mainly consisting of chemical reagents; analysis kits used in science for immobilization of fusion peptides, mainly consisting of chemical reagents

Abrégé

The invention provides new methods of isolating target cells using a solid phase, the solid phase comprising a ligand L wherein the ligand L is capable of specifically binding a ligand binding partner LB, the ligand binding partner LB being present in a receptor molecule binding reagent or a multimerization reagent used for isolating target cells. The invention also provides corresponding new arrangements and devices for isolating a target cell from a sample.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/566 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet utilisant un support spécifique ou des protéines réceptrices comme réactifs pour la formation de liaisons par ligand

Abrégé

The invention concerns novel streptavidin muteins. In one embodiment such a mutein (a) contains at least one mutation in the region of the amino acid positions 115 to 121 with reference to the amino acid sequence of wild type streptavidin as set forth at SEQ ID NO: 15 and (b) has a higher binding affinity than each of (i) a streptavidin mutein that comprises the amino acid sequence Val44 -Thr45- Ala46-Arg47 (SEQ ID NO: 98), or (ii) a streptavidin mutein that comprises the amino acid sequence He44-Gly45- Ala46-Arg47 (SEQ ID NO: 99) at amino acid positions 44 to 47, or (iii) wild type-streptavidin (SEQ ID NO: 15) for peptide ligands comprising the amino acid sequence Trp-Ser-His-Pro-Gln- Phe-Glu-Lys (SEQ ID NO: 100).

Classes IPC  ?

• C07K 14/36 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant d'ActinomycesPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Streptomyces (G)

Abrégé

Disclosed is a bifunctional adapter molecule comprising two binding moieties A and B, the adapter molecule being capable of reversibly equipping a fusion protein carrying an oligohistidine affinity tag with a further affinity tag, wherein the binding moiety A comprises at least two chelating groups K, wherein each chelating group is capable of binding to a transition metal ion, thereby rendering moiety A capable of binding to an oligohistidine affinity tag, and the binding moiety B is an affinity tag other than an oligohistidine tag.

Classes IPC  ?

• C07K 1/13 - Marquage de peptides
• C07K 1/22 - Chromatographie d'affinité ou techniques analogues basées sur des procédés d'absorption sélective
• C07K 14/36 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant d'ActinomycesPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Streptomyces (G)
• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides

Abrégé

The present invention relates to methods of reversibly staining a target cell. The invention also relates to methods of isolating a target cell or a target cell population that is defined by the presence of at least one common specific receptor molecule. The invention also provides kits that can be used to carry out the methods of the invention.

Classes IPC  ?

• C07K 14/36 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant d'ActinomycesPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Streptomyces (G)
• G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique

Produits et services

Chemicals used in industry and science, in particular reagents for the cloning of nucleic acids, reagents for the amplification of nucleic acids, reagents for the mutagenesis of nucleic acids, reagent kits containing oligonucleotides, enzymes and buffers for the amplification of nucleic acids, reagent kits containing oligonucleotides, enzymes and buffers for mutagenesis of nucleic acids, single and double stranded DNA, plasmid DNA, oligonucleotides, enzymes for the cloning of nucleic acids, buffers for the cloning of nucleic acids, enzymes for the amplification of nucleic acids, buffers for the amplification of nucleic acids, enzymes for the mutagenesis of nucleic acids, buffers for the mutagenesis of nucleic acids, reagents for purification and/or tagging and/or detection and/or immobilization and/or functionalization of peptides and proteins including fusions proteins, reagent kits containing affinity materials and buffers for the purification and/or tagging and/or detection and/or immobilization and/or functionalization of peptides and proteins including fusion proteins, analytic kits and test reagents used in industry and science, diagnostic reagents (except for medical use). Pharmaceutical and veterinary preparations, in particular nucleic acids and peptides and proteins, including fusion proteins, said peptides and proteins produced by genetic engineering, diagnostic reagents for medical use. Research in science and industry in the areas of biochemistry, diagnostics and pharmaceuticals; services of a biochemical or pharmaceutical laboratory, in particular cloning, amplification and mutagenesis of nucleic acids, purification and/or tagging and/or detection and/or immobilization and/or functionalization of peptides and proteins including fusions peptides.

Produits et services

chemicals used in biopharmaceutical industry and science for the isolation of body cells and cells of the immune system of mammals, for scientific, medical or laboratory research; diagnostic reagents for scientific or research use; analytic kits comprising streptavidin or streptavidin muteins carrying a fluorescent and/or magnetic label and/or recombinant MHC I or MHC II molecules fused to an affinity label, binding to such labeled streptavidin or streptavidin muteins, used for the isolation of T cells under maintenance of their authentic properties pharmaceutical, medical and veterinary preparations, namely, polypeptides and proteins produced by genetic engineering, including fusion proteins, and body cells and cells of the immune system of mammals, all used for the treatment of graft rejections and/'or viral infections and/or cancer by providing functional, antigen specific T cells for adoptive transfer, for the monitoring of the functional T cell status after vaccinations; medical diagnostic reagents [scientific and industrial research in the fields of biochemistry, medical diagnostics and pharmaceuticals; biochemical and pharmaceutical laboratory services featuring the synthesis of recombinant polypeptides and proteins, cultivator and purification of cells and cells of the immune system of mammals including human cells]