Abrégé

The present invention relates to a lateral flow assay sensor that increases detection sensitivity of the sensor by adding a bridge structure. The lateral flow assay sensor of the present invention is a lateral flow assay sensor that forms channels on a paper by using printing technology, comprising: a sample injection channel into which a sample is injected; a diagnosis channel spaced apart from one end of the sample injection channel by a predetermined distance so as to be elongated in the vertical direction, and having a diagnosis region coated with a detection material in the middle thereof; a bridge pad superimposed on each of the sample injection channel and the diagnosis channel so as to form a bridge for moving the sample from the sample injection channel to the diagnosis channel; and an absorption pad positioned on the top of the diagnosis channel to absorb a developing solution.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/558 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet utilisant la diffusion ou la migration de l'anticorps ou de l'antigène

Abrégé

The present invention relates to a pattern structure of a diagnostic sensor, wherein the pattern is formed by printing an organic mixture on paper. More particularly, the present invention relates to a pattern structure of a diagnostic sensor, wherein the pattern structure enables detection and diagnosis to be performed by transferring two kinds of materials at different times so that the two materials react without interference from each other. For example, a first material and a second material can be transferred to a diagnosis area at different times by different fluid flows, and reaction of the second material can be induced in the diagnosis area without interference from the first material that was supplied earlier.

Classes IPC  ?

• G01N 35/08 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant un courant d'échantillons discrets circulant dans une canalisation, p. ex. analyse à injection dans un écoulement
• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes

Abrégé

A multiple molecular diagnosis chip structure according to one embodiment of the present invention comprises: a reaction pad having a sample passage hole formed therethrough and a plurality of reaction patterns formed thereon, wherein an injected sample to be diagnosed can pass through the sample passage hole, and a reaction solution that includes an isothermal amplification primer for a specific nucleic acid to be diagnosed, an amplification enzyme, and an indicator having fluorescence varying depending on amplification exists in the reaction patterns; a channel pad disposed on the lower surface of the reaction pad, the channel pad having a first sample passage channel formed in a position corresponding to the sample passage hole, and a second sample passage channel corresponding to the reaction patterns; and a transfer pad disposed on the lower surface of the channel pad to transfer a sample, which is injected from the sample passage hole and transferred through the first sample passage channel, to the reaction patterns through the second sample passage channel.

Classes IPC  ?

• B01L 7/00 - Appareils de chauffage ou de refroidissementDispositifs d'isolation thermique
• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques

Abrégé

An embodiment of the present invention relates to a method for manufacturing a membrane sensor, wherein the method may comprise the steps of: using a craft printer to manufacture a paper plate in which a pattern is formed on paper; soaking the paper plate in an organic solvent; attaching the paper plate to a membrane through which a water-soluble sample can flow, thereby stamping the paper plate to the membrane; removing the paper plate from the membrane; and drying the membrane for a period of time. Therefore, a bio-sensor can be manufactured by forming a plurality of various design patterns on a membrane using a simple method of stamping, on a membrane, a paper plate soaked with an organic solvent.

Classes IPC  ?

• G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• C08B 5/02 - Nitrate de cellulose

Abrégé

The present invention relates to an immunochromatography strip sensor capable of measuring a biomaterial concentration over a broad concentration range, and a method for measuring a biomaterial concentration over a broad concentration range using the sensor. A detection method using the sensor of the present invention can accurately measure an antigen concentration over a broad concentration range, and achieve a low cost, rapidity and convenience, and thus the method is suitable for a point of care test (POCT) requiring rapidity and high sensitivity.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/563 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet faisant intervenir des fragments d'anticorps

Abrégé

Provided is a method for manufacturing a multiple-diagnosis membrane sensor provided with multiple channels by using screen printing, and more specifically, to a method for manufacturing a membrane sensor capable of performing multiple-diagnosis by screen-printing hydrophobic ink on a membrane to form multiple channels. The membrane sensor according to the present invention may enable mass-production of sensors and secure reliability of detection by forming the plurality of channels on the membrane by a simple method.

Classes IPC  ?

• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
• G01N 33/558 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet utilisant la diffusion ou la migration de l'anticorps ou de l'antigène
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques

Abrégé

Disclosed herein are a membrane sensor capable of changing a reaction condition by a single sample injection and a method for measuring a reaction using the same, and more specifically, a membrane sensor designed so that a bio reaction having two or more reaction conditions is sequentially generated by a single sample injection, by forming an asymmetric membrane between a reactant storing part and a reaction membrane.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• G01N 33/544 - Support organique
• G01N 33/558 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet utilisant la diffusion ou la migration de l'anticorps ou de l'antigène

Abrégé

The present invention relates to a membrane sensor enabled with a sequential change of a reaction condition with a single sample injection and to a method for measuring the reaction using same, and more specifically, to a membrane sensor, which is designed so that a vital reaction having two or more reaction conditions that occur sequentially with a single sample injection, by forming an asymmetric film between a reaction substance storing portion and a reaction membrane.

Classes IPC  ?

• G01N 33/538 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec formation d'un complexe immunologique en phase liquide avec séparation du complexe immunologique de l'antigène ou de l'anticorps non liés par colonne, particules ou bande de résine synthétique absorbantes ou adsorbantes
• G01N 33/52 - Utilisation de composés ou de compositions pour des recherches colorimétriques, spectrophotométriques ou fluorométriques, p. ex. utilisation de bandes de papier indicateur
• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides

Abrégé

The present invention relates to a method for manufacturing a multiple-diagnosis membrane sensor provided with multiple channels by using screen printing, and more specifically, to a method for manufacturing a membrane sensor capable of multiple diagnosis by means of forming multiple channels by screen-printing a hydrophobic ink on top of a membrane. The membrane sensor according to the present invention can allow mass-production of sensors and secure reliability of detection by forming the plurality of channels on top of the membrane by means of a simple method.

Classes IPC  ?

• G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
• G01N 33/535 - Production de composés immunochimiques marqués avec un marqueur enzymatique
• G01N 33/52 - Utilisation de composés ou de compositions pour des recherches colorimétriques, spectrophotométriques ou fluorométriques, p. ex. utilisation de bandes de papier indicateur

Abrégé

The present invention relates to a membrane sensor having a multi-hole film attached thereto and a method for measuring immunological reactions or enzymatic reactions using the same. More specifically, the present invention relates to a membrane sensor in which a multi-hole film is joined to the top of a membrane on which receptors are immobilized, and a method for measuring immunological reactions or enzymatic reactions using the same. The present invention makes it possible to adjust the sensitivity of membrane biosensors by adjusting the hole size in the multi-hole film and so makes it possible to measure analytes with a high degree of sensitivity using just a small amount of sample, and makes it possible to simultaneously measure diverse types of analyte by attaching various types of receptor on the membrane sensor.

Classes IPC  ?

• C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
• G01N 33/538 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec formation d'un complexe immunologique en phase liquide avec séparation du complexe immunologique de l'antigène ou de l'anticorps non liés par colonne, particules ou bande de résine synthétique absorbantes ou adsorbantes
• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques