The invention relates to methods and kits for the detection of pea rot pathogens in a soil sample using a loop-mediated isothermal amplification (LAMP) assay, where the assay comprises primers for at least one pea rot pathogen.
C12Q 1/6895 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les plantes, les champignons ou les algues
The present invention relates to methods of improving abiotic stress tolerance, and specifically, maintaining the fertility of plants under stress, and in particular heat stress. Such methods comprise introducing one or more mutations into at least one nuclear localized cyclic nucleotide-gated ion channel (CNGC) gene.
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
Described is a method for determining structure of an RNA molecule, the method comprising: a) subjecting a population of RNA molecules to structure-specific chemical modifications such that individual RNA molecules are modified; b) reverse-transcribing the modified RNA to provide a complementary DNA (cDNA) molecule, and generating double stranded DNA from said cDNA molecule; c) performing single-molecule sequencing of the double stranded cDNA using a sequencing format which provides multiple reads of each molecule to arrive at a consensus sequence representing a chemical mutation profile for an individual DNA; d) using said chemical mutation profile to determine likelihood of an RNA molecule being single stranded or double stranded at each individual base, to thereby determine the structure of the RNA molecule.
The invention relates to methods and systems for delivering RNA to cells using virus-like particles (VLPs) and scaffold-based binding proteins (SBPs); as well as applications for such methods and systems, for example in therapeutics and diagnostics.
A61K 39/00 - Préparations médicinales contenant des antigènes ou des anticorps
A61K 48/00 - Préparations médicinales contenant du matériel génétique qui est introduit dans des cellules du corps vivant pour traiter des maladies génétiquesThérapie génique
C12N 7/00 - Virus, p. ex. bactériophagesCompositions les contenantLeur préparation ou purification
A61K 39/00 - Préparations médicinales contenant des antigènes ou des anticorps
A61K 48/00 - Préparations médicinales contenant du matériel génétique qui est introduit dans des cellules du corps vivant pour traiter des maladies génétiquesThérapie génique
C12N 7/00 - Virus, p. ex. bactériophagesCompositions les contenantLeur préparation ou purification
The present invention relates to a modified secreted AY-WB protein 5 (SAP05) protein, and in particular the use of the modified SAP05 protein as part of a fusion compound for use in ubiquitin-independent protein degradation. The invention also relates to methods for the use of the fusion compound in targeted protein degradation, modulating physiological responses and therapy.
Methods for increasing levels of provitamin D3 and/or vitamin D3 in plants are described. Tomato plants were genetical modified resulting in decreased activity of 7-dehydrocholesterol reductase.
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
A01H 3/02 - Procédés de modification des phénotypes par contrôle de la durée, de la longueur d'onde, de l'intensité ou de la périodicité de l'éclairement
The present invention relates to methods of cleaving target molecules comprising a heterologous RimB recognition motif, the method comprising applying a protease. The presently disclosed invention is particularly useful in methods of tag removal, modulating protein activity, and selective cleavage of a polypeptide.
The invention relates to genetically altered bacteria with autoactive nucleotide-binding oligomerisation domain-like receptor (NLR) proteins or NLR-like proteins, and metabolites produced by such bacteria. The invention also relates to methods of producing such genetically altered bacteria.
C07K 14/315 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Streptococcus (G), p. ex. Enterocoques
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 15/67 - Méthodes générales pour favoriser l'expression
C12P 21/02 - Préparation de peptides ou de protéines comportant une séquence connue de plusieurs amino-acides, p. ex. glutathion
C12P 1/00 - Préparation de composés ou de compositions, non prévue dans les groupes , utilisant des micro-organismes ou des enzymesProcédés généraux de préparation de composés ou de compositions utilisant des micro-organismes ou des enzymes
Provided are compounds useful for treating bacterial infections, e.g. Gram-negative bacterial infection. The compounds may be of formula (I) as defined herein. The compounds may be capable of binding a horseshoe-like hydrophobic pocket on the DNA-binding 5 surface GyrA subunit of DNA gyrase. Also provided are formulations comprising such compounds, as well uses of such compounds or formulations, e.g. for the treatment of bacterial infection, and/or as a DNA gyrase inhibitor.
C07D 217/02 - Composés hétérocycliques contenant les systèmes cycliques de l'isoquinoléine ou de l'isoquinoléine hydrogénée avec uniquement des atomes d'hydrogène ou des radicaux ne contenant que des atomes d'hydrogène et de carbone, liés directement aux atomes de carbone du cycle contenant l'azoteAlkylène-bis-isoquinoléines
The present invention relates to a fusion protein for use in ubiquitin-independent protein degradation. The invention also relates to methods for the use of the fusion protein in targeted protein degradation, modulating physiological responses and therapy.
The invention relates to genetically altered plants, parts thereof or plant cells, where the plants, parts thereof or plant cells are characterised by increased levels of iron. Also described are methods of increasing the levels of iron in a plant, particularly a crop plant as well as food compositions and nutritional supplements obtained from the plants or parts thereof.
Provided are compositions and methods for improving gene editing efficiency in plants. Methods and compositions are also provided for producing modifications using novel Cas12a nuclease variants. Modified plant cells and plants comprising DNA and protein compositions of novel Cas12a nuclease variants are further provided.
C12Q 1/6895 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les plantes, les champignons ou les algues
15.
COMPOSITIONS AND METHODS FOR INCREASING GENOME EDITING EFFICIENCY
Provided are compositions and methods for improving gene editing efficiency in plants. Methods and compositions are also provided for producing modifications using novel Cas12a nuclease variants. Modified plant cells and plants comprising DNA and protein compositions of novel Cas12a nuclease variants are further provided.
Provided are compositions and methods for improving gene editing efficiency in plants. Methods and compositions are also provided for producing modifications using novel Cas12a nuclease variants. Modified plant cells and plants comprising DNA and protein compositions of novel Cas12a nuclease variants are further provided.
Described is a method for determining structure of an RNA molecule, the method comprising: a) subjecting a population of RNA molecules to structure-specific chemical modifications such that individual RNA molecules are modified; b) reverse-transcribing the modified RNA to provide a complementary DNA (cDNA) molecule, and generating double stranded DNA from said cDNA molecule; c) performing single-molecule sequencing of the double stranded cDNA using a sequencing format which provides multiple reads of each molecule to arrive at a consensus sequence representing a chemical mutation profile for an individual DNA; d) using said chemical mutation profile to determine likelihood of an RNA molecule being single stranded or double stranded at each individual base, to thereby determine the structure of the RNA molecule.
The present invention relates to genetically altered plants, parts thereof and plant cells that comprises one or more mutations in a conserved motif XDPX of nuclear localized cyclic nucleotide-gated ion channel (CNGC) channels, as well as methods of increasing yield, nodulation and arbuscular mycorrhiza (AM) endosymbioses by introducing the one or more mutation into a CNGC gene.
The present invention relates to genetically altered plants, parts thereof and plant cells that comprises one or more mutations in a conserved motif XDPX of nuclear localized cyclic nucleotide-gated ion channel (CNGC) channels, as well as methods of increasing yield, nodulation and arbuscular mycorrhiza (AM) endosymbioses by introducing the one or more mutation into a CNGC gene.
Methods for increasing levels of provitamin D3 and/or vitamin D3 in plants are described. Tomato plants were genetical modified resulting in decreased activity of 7-dehydrocholesterol reductase.
The invention relates to methods of engineering a host to become resistant or increase the resistance of a host to a vector-borne pathogen. In one example, there is a provided a method of engineering a plant to become resistant to phytoplasma.
The present invention relates to a fusion protein for use in ubiquitin-independent protein degradation. The invention also relates to methods for the use of the fusion protein in targeted protein degradation, modulating physiological responses and therapy.
The present invention relates to methods for determining likely vernalisation requirements for plants, and to methods for selecting plants for breeding to produce a desired vernalisation requirement.
The invention is directed to a method for identifying one or more genes associated with a selected phenotype in an organism. The method includes the steps of providing sequence information derived from a database of genetic samples from a population of the organism, wherein the genetic samples comprise nucleic acids, and wherein the sequence information is comprised of a plurality of contigs, identifying sub-sequences of a fixed length k (k-mers) associated with the selected phenotype within the sequence information, ordering one or more k-mers using a reference genome assembly, and mapping the k-mers onto one or more of the ordered contigs to identify one or more genes associated with the selected phenotype.
The present invention relates to a method of producing L-DOPA in plants, and in particular, in non-betalain-producing plants. The invention also relates to plants obtained by the method, as well as the fruits thereof, and pharmaceutical compositions comprising L-DOPA obtained from the plants of the invention.
The complex chemistry underlying the extensive transformations involved in terpene indole alkaloid synthesis makes identification of the biosynthetic genes challenging. The present invention relates to methods for producing a terpene indole alkaloid derivative, comprising the steps of: (1) providing a terpene indole alkaloid; and (2) providing a first enzyme termed “Precondylocarpine Acetate Synthase” (PAS) or a functional variant or homologue thereof; and/or a second enzyme termed “Dehydroprecondylocarpine Acetate Synthase” (DPAS) or a functional variant or homologue thereof, and optionally providing further identified enzymes involved in this pathway. The invention also encompasses related kits, enzymes, expression vectors, host cells and plants.
C12P 17/18 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle contenant plusieurs hétérocycles condensés entre eux ou condensés avec un système carbocyclique commun, p. ex. rifamycine
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
The invention relates to enzymes and methods for producing a monoterpenoid compound. In one aspect, the invention is a method for producing a monoterpenoid compound, comprising the steps of (1) providing a monoterpenoid precursor; (2) providing a NEPS enzyme; and (3) contacting the monoterpenoid precursor with the enzyme under catalytic conditions to produce an monoterpenoid compound.
C12P 17/06 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle contenant un hétérocycle à six chaînons, p. ex. fluorescéine
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
The invention relates to methods of increasing biotic stress resistance in a plant as well as plants with increased biotic stress resistance and methods of screening plants for the beneficial phenotype
The invention relates to enzymes and methods for producing a monoterpenoid compound. In one aspect, the invention is a method for producing a monoterpenoid compound, comprising the steps of (1) providing a monoterpenoid precursor; (2) providing a NEPS enzyme; and (3) contacting the monoterpenoid precursor with the enzyme under catalytic conditions to produce an monoterpenoid compound.
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
A01N 65/22 - Lamiaceae ou Labiatae [famille de la menthe], p. ex. thym, romarin, scutellaire, brunelle, lavande, perilla, menthe pouliot, menthe poivrée ou menthe verte
C07D 311/94 - Composés hétérocycliques contenant des cycles à six chaînons comportant un atome d'oxygène comme unique hétéro-atome du cycle, condensés avec d'autres cycles condensés en ortho ou en péri avec des carbocycles ou avec des systèmes carbocycliques condensés avec des cycles autres que des cycles à six chaînons ou avec des systèmes cycliques contenant de tels cycles
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
31.
GENES ASSOCIATED WITH RESISTANCE TO WHEAT YELLOW RUST
COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION (Australie)
JOHN INNES CENTRE (Royaume‑Uni)
Inventeur(s)
Uauy, Cristobal
Marchal, Clemence
Lagudah, Evans
Mcintosh, Robert
Zhang, Jianping
Zhang, Peng
Abrégé
The invention relates to genes associated with disease resistance in plants. According to an aspect of the invention is provided an isolated nucleic acid encoding a nucleotide-binding and leucine-rich repeat (NLR) polypeptide comprising a zinc-finger BED domain, wherein expression of the NLR polypeptide in a plant confers or enhances resistance of the plant to a fungus, for example wheat yellow (stripe) rust fungus Puccinia striiformisi f. sp. tritici.
The complex chemistry underlying the extensive transformations involved in terpene indole alkaloid synthesis makes identification of the biosynthetic genes challenging. The present invention relates to methods for producing a terpene indole alkaloid derivative, comprising the steps of: (1) providing a terpene indole alkaloid; and (2) providing a first enzyme termed "Precondylocarpine Acetate Synthase" (PAS) or a functional variant or homologue thereof; and/or a second enzyme termed "Dehydroprecondylocarpine Acetate Synthase" (DPAS) or a functional variant or homologue thereof, and optionally providing further identified enzymes involved in this pathway. The invention also encompasses related kits, enzymes, expression vectors, host cells and plants.
C07D 519/04 - Alcaloïdes de dimères d'indole, p. ex. vincaleucoblastine
C07D 471/22 - Composés hétérocycliques contenant des atomes d'azote comme uniques hétéro-atomes du système condensé, au moins un cycle étant un cycle à six chaînons avec un atome d'azote, non prévus dans les groupes dans lesquels le système condensé contient au moins quatre hétérocycles
C12P 17/18 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle contenant plusieurs hétérocycles condensés entre eux ou condensés avec un système carbocyclique commun, p. ex. rifamycine
C07D 459/00 - Composés hétérocycliques contenant des systèmes cycliques benz [g] indolo [2, 3-a] quinolizine, p. ex. yohimbineLeurs lactones en 16, 18, p. ex. lactone de l'acide réserpique
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
34.
METHOD FOR IDENTIFYING GENES ASSOCIATED WITH A PARTICULAR PHENOTYPE
kkkk-mers. The method may be used for example to identify genes encoding plant resistance proteins, such as nucleotide-binding and leucine-rich repeat (NLR) proteins.
A method of processing images of a crop, particularly a cereal crop, is described. The method comprises retrieving a series of images of a crop (3; Fig. 2) captured over a period of time and identifying, in an image selected from the series of images to be used as reference image, a reference system against which other images can be compared, the reference system including an extent of a crop plot (111; Fig. 23) and/or one or more reference points, such as height markers (107; Fig. 21). The method also comprises, for each of at least one other image in the series of images, calibrating the image using the reference system, and determining a height of a canopy of the crop in the image, a main orientation of the crop and/or a value indicative of vegetative greenness.
brevets
