The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently produce carbon- based products of interest, and in particular the use of such organisms for the commercial production of ethanol and related carbon-based molecules in the presence of contaminating microorganisms which can utilize the same. Other materials, methods, and compositions are also described.
The present disclosure provides methods and compositions for modifying photosynthetic organisms, such that the organisms efficiently produce carbon-based products such as alkanes, and in particular the use of such organisms for the commercial production of carbon-based products and related molecules.
C12P 7/64 - GraissesHuilesCires de type esterAcides gras supérieurs, c.-à-d. ayant une chaîne continue d'au moins sept atomes de carbone liée à un groupe carboxyleHuiles ou graisses oxydées
C12P 7/06 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique
C12P 5/02 - Préparation des hydrocarbures acycliques
A method and system for recovering a volatile organic compound from a dilute aqueous phase. The method may include separating volatile organic compound from the aqueous phase by using carrier gas to generate a solvent-laden vapor stream, feeding a solvent-laden vapor stream to a mass of carbon adsorbent and enabling the solvent to be absorbed and separated from the solvent-laden vapor stream, releasing the absorbed volatile organic compound, and condensing the released volatile organic compound to form a condensate. The system may include a vapor phase source containing ethanol, at least one carbon bed containing a mass of coconut shell carbon, a steam source in fluid communication with the carbon bed, and a condenser in fluid communication with the carbon bed. The method and system may also utilize microbeads as an absorbent and may be configured so the capacity is scalable from lab scale to production scale.
The present invention provides a photobioreactor for a phototrophic microorganism and culture medium therefor. The photobioreactor for a phototrophic microorganism and culture medium may include an elongated capsule having a flexible polymer film that is at least partially transparent to light of a wavelength that is photosynthetically active in a phototrophic microorganism. The elongated capsule can be divided widthwise into a plurality of adjacent channels, each channel being comprised of the flexible polymer film and having in a plurality of positions distributed along a length of the channel a reinforced material bonded with the channel, the reinforced material surrounding part or the entire perimeter of the channel, independently, in the each of the plurality of positions.
The present disclosure provides methods and compositions for modifying a photosynthetic organism, such that the gene expression in the organism is regulated by cumate exposure. The disclosure also describes the use of such organisms for production of a carbon-based product of interest.
The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently produce alkanes, and in particular the use of such organisms for the commercial production of alkanes and related molecules. Other materials, methods, and compositions are also described. Many existing photoautotrophic organisms (i.e., plants, algae, and photosynthetic bacteria) are poorly suited for industrial bioprocessing and have therefore not demonstrated commercial viability. Recombinant photosynthetic microorganisms have been engineered to produce hydrocarbons and alcohols in amounts that exceed the levels produced naturally by the organism.
The invention relates to a photobioreactor system for a phototrophic microorganism, and culture medium therefor, comprising a reactor chamber and a thermal system. The thermal system includes a convection chamber in thermal contact with the reactor chamber and having a first port and a second port; a heat storage reservoir having a first region containing a first volume of heat exchange liquid at a first temperature and a second region containing a second volume of heat exchange liquid at a second temperature; and a flow system configured for (1) flowing heat exchange liquid from the heat storage reservoir into the first port and through the convection chamber and flowing heat exchange liquid from the convection chamber out of the second port into the heat storage reservoir; and (2) flowing heat exchange liquid from the heat storage reservoir into the second port and through the convection chamber and flowing heat exchange liquid from the convection chamber out of the first port into the heat storage reservoir. Methods of using said photobioreactor to manage the temperature of the culture medium in the photobioreactor and to manage the amount of heat stored in the reservoir are also described.
The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into pyruvate or acetyl-CoA, and in particular the use of such organisms for the commercial production of molecules derived from these precursors, e.g., ethanol.
C12P 7/06 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique
C12N 1/12 - Algues unicellulairesLeurs milieux de culture
C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
9.
EXTRACELLULAR TRANSPORT OF BIOSYNTHETIC HYDROCARBONS AND OTHER MOLECULES
Disclosed are methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into hydrocarbons, e.g., n-alkanes and n-alkenes, wherein the n-alkanes are secreted into the culture medium via recombinantly expressed transporter proteins. The use of such organisms for the commercial production of n-alkanes and related molecules is contemplated.
The present disclosure identifies pathways and mechanisms to confer resistance to reactive oxygen species, e.g., hydrogen peroxide, to photoautotrophic organisms. The use of such organisms in host cell culture systems with high levels of reactive oxygen species, such as host cell culture systems decontaminated with hydrogen peroxide, is contemplated.
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12P 7/06 - Éthanol en tant que produit chimique et non en tant que boisson alcoolique
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
11.
POLYMERIC THIN-FILM TUBE CONNECTORS, BIOREACTORS, SYSTEMS AND METHODS
Polymeric thin-film tube connectors, bioreactors, systems and methods are described, the connectors allowing connection of a polymeric thin-film tube of a bioreactor chamber with a complementary part, typically, rigid port of flange of the bioreactor or bioreactor system. Further, bioreactors having a thin-film reactor chamber and one or more connectors are described. The thin-film reactor chamber can have a thin-film wall for enclosing culture medium and microorganisms. The connector can include a flexible boot. An opening of the thin-film wall couples to the flexible boot. The thin-film wall can extend through a reactor end of the flexible boot and can rest against an interior surface of the flexible boot. The connectors are particularly advantageous for connecting standard utilities in a bioreactor system to thin-film bioreactor chambers or capsules having a thin-film tubular opening.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/24 - Appareillage pour l'enzymologie ou la microbiologie en forme de tube ou de bouteille
C12M 3/02 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus comportant des moyens fournissant des suspensions
C12N 1/12 - Algues unicellulairesLeurs milieux de culture
Various 1-alkenes, including 1-nonadecene and 1-octadecene, are synthesized by the engineered microorganisms and methods of the invention. In certain embodiments, the microorganisms comprise a recombinant alpha-olefin-associated enzyme. This enzyme may be expressed in combination with a recombinant alkene synthase pathway-related gene. The engineered microorganisms may be photosynthetic microorganisms such as cyanobacteria.
C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques
The present invention provides a photobioreactor for a phototrophic microorganism, and culture medium therefor, comprising a capsule, the capsule comprising an elongated body comprised of a first, flexible polymer film that is at least partially transparent to light of a wavelength that is photosynthetically active in a phototrophic microorganism and divided width wise into a plurality of adjacent channels, each channel having a major cross-sectional dimension that is not more than about one-third of the length of the elongated body, when the elongated body is inflated; and a fluid distribution structure coupled to the elongated body adapted for fluid communication with the plurality of adjacent channels that distributes a flow of culture medium amongst the plurality of channels, wherein the fluid distribution structure is comprised of a second, flexible polymer film.
Bioreactors, and particularly, photobioreactors having a reactor chamber and surge driver, and methods for using these devices, for example, for the production of carbon-based products are provided. The reactor chamber provides a housing for microorganisms and culture medium. The surge driver produces a surge of the microorganisms and/or culture medium in the reactor chamber.
The present disclosure provides compositions and methods for genetic marker removal in cyanobacteria. Cyanobacteria are developed having a genetic marker capable of being selected against in the presence of a negatively selective condition, resulting in removal of a positively selective marker (e.g., an antibiotic resistance marker) from the host cell.
Systems and methods for the production of carbon dioxide are provided that can be used directly at a bioreactor site and that can provide release and delivery of carbon dioxide in a cost-efficient manner. The methods and systems can use solar energy for the thermal dehydration or desorption of a carbon dioxide source. Further, methods and bioreactor plants for the production of carbon-based products are provided that can include the methods and systems for the production of carbon dioxide, and can allow reduced carbon-based product cost due to reduced carbon dioxide associated costs and further can allow for energy efficient temperature control of bioreactors.
The present invention provides a bioreactor having a reactor chamber and one or more support chambers. The reactor chamber can have one or more flexible walls for enclosing microorganisms and culture medium. The reactor chamber provides an enclosure for microorganism and culture medium. The support chamber can also have one or more flexible walls. When inflated to a predetermined amount, the support chamber causes the microorganisms and culture medium to distribute to a substantially even depth across the reactor chamber.
The present disclosure provides compositions and methods for genetic marker removal in cyanobacteria. Cyanobacteria are developed having a genetic marker capable of being excised endogenously with a recombinase protein. Subsequent plasmid segregation removes genetic marker(s) and nucleotide sequences expressing recombinase from the host cell.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12N 15/74 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert inorganic carbon and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules.
The NonA alkene synthase in Synechococcus sp. displays selective synthesis of 1- nonadecene. Heterologous recombination of a domain, i.e. the acyl binding domain, with other acyl binding proteins, affects acyl substrate chain-length binding selectivity and therefore the chain-length of the synthesized 1-alkenes. Compositions and methods are provided to selectively synthesize 1-alkenes of various chain lengths.
The present invention provides compositions and methods for controlling biosynthetic pathways using a metabolic switch in microorganisms. Photoautotrophs are developed to be auxotrophic for certain exogenous compounds such as lipoic acid and/or a fixed nitrogen source. Depletion of the exogenous compound results in the carbon flux to be diverted to preferred metabolic pathways.
C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
22.
METHODS AND COMPOSITIONS FOR THE RECOMBINANT BIOSYNTHESIS OF TERMINAL OLEFINS
The present disclosure identifies methods and compositions for modifying microbial cells, such that the organisms efficiently synthesize terminal olefins, and in particular the use of such organisms for the commercial production of propylene and related molecules.
C12P 5/02 - Préparation des hydrocarbures acycliques
C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
23.
METHODS AND COMPOSITIONS FOR LIMITING VIABILITY OF A MODIFIED HOST CELL OUTSIDE OF DESIGNATED PROCESS CONDITIONS
The invention provides methods and compositions for inhibiting proliferation of a modified host cell outside of a designated process condition. Compositions and methods for providing a host cell having reduced viability when exposed to natural conditions external to a controlled environment are disclosed.
The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into hydrocarbons, e.g., n-alkanes and n-alkenes, wherein the n-alkanes are secreted into the culture medium via recombinantly expressed transporter proteins. In particular, the use of such organisms for the commercial production of n-alkanes and related molecules is contemplated.
C12P 1/04 - Préparation de composés ou de compositions, non prévue dans les groupes , utilisant des micro-organismes ou des enzymesProcédés généraux de préparation de composés ou de compositions utilisant des micro-organismes ou des enzymes utilisant des bactéries
This disclosure pertains to the functional localization of heterologous integral plasma membrane proteins (HIPMPs) lacking cleavable signal sequences into the plasma membrane (PM) of cyanobacterial hosts, e.g., JCC138 (Synechococcus sp. PCC 7002) or an engineered derivative thereof. More specifically, the disclosure provides chimeric integral plasma membrane proteins comprising pseudo leader sequences (PLSs) that promote increased hydrocarbon (e.g., alkane) export capabilities when expressed in a photosynthetic organism, e.g., a cyanobacterium.
The invention provides methods and compositions for the improved biosynthesis of ethanol in a host cell. Genes encoding enzymes for futile cycle pathways to increase ethanol production, methods of optimizing expression of these enzymes in host cells, and methods for the production of ethanol by these host cells are disclosed. Exogenous compounds for increasing ethanol productivity by the host cells are also provided.
The invention provides methods for extracting carbon-based molecules of interest produced by host cells cultured in a biphasic system. Methods and apparatus for extracting a carbon-based molecule from a host cell both in the presence of and in the absence of a cyclodextrin in a biphasic system is disclosed. The method provides for continuous production of carbon-based molecules of interest by host cells.
C07J 1/00 - Stéroïdes normaux contenant du carbone, de l'hydrogène, un halogène ou de l'oxygène, non substitués en position 17bêta par un atome de carbone, p. ex. œstrane, androstane
C08B 31/00 - Préparation des dérivés chimiques de l'amidon
C08B 33/00 - Préparation des dérivés chimiques de l'amylose
C08B 35/00 - Préparation des dérivés chimiques de l'amylopectine
28.
METHODS AND COMPOSITIONS FOR THE RECOMBINANT BIOSYNTHESIS OF PROPANOL
The invention provides methods for the biosynthesis of propanol using engineered host cells. Genes encoding enzymes for the conversion of metabolic intermediates to propanol, methods for optimizing expression of these enzymes in host cells, and methods for the production of propanol by these host cells are disclosed.
The present invention provides photobioreactors, solar energy gathering systems, and methods for thermal control of a culture medium containing a prototrophic organism in a photobioreactor, that allow temperature control in a cost effective manner, reducing the energy required for temperature control of a culture medium containing phototrophic microorganisms in a photobioreactor.
The present invention provides fractional condensation apparatuses, systems and methods that can recover volatile products from highly diluted liquid and vapor feed mixtures with high purity and recovery yield, both in batch and continuous distillation, while being also designed to achieve low-cost and efficient recovery processes. Single apparatuses and columns can be connected to each other to expand or shrink the column length for desired separation. The apparatuses, systems and methods can be applied to separate mixtures with both narrow and wide boiling point ranges.
F25J 3/02 - Procédés ou appareils pour séparer les constituants des mélanges gazeux impliquant l'emploi d'une liquéfaction ou d'une solidification par rectification, c.-à-d. par échange continuel de chaleur et de matière entre un courant de vapeur et un courant de liquide
F25J 3/08 - Séparation des impuretés gazeuses des gaz ou des mélanges gazeux
B01D 3/00 - Distillation ou procédés d'échange apparentés dans lesquels des liquides sont en contact avec des milieux gazeux, p. ex. extraction
C10G 1/02 - Production de mélanges liquides d'hydrocarbures à partir de schiste bitumineux, de sable pétrolifère ou de matières carbonées solides non fusibles ou similaires, p. ex. bois, charbon par distillation
C10G 7/00 - Distillation des huiles d'hydrocarbures
31.
METHODS AND COMPOSITIONS FOR CONTROLLING CONTAMINATION GROWTH IN CELL CULTURES
The present invention provides compositions and methods for controlling the growth of contaminating microbes in cell cultures, particularly cultures of photosynthetic organisms used to produce bio fuels and other carbon-based products of interest.
C12N 1/38 - Stimulation chimique de la croissance ou de l'activité par addition de composés chimiques qui ne sont pas des facteurs essentiels de croissanceStimulation de la croissance par élimination d'un composé chimique
Improved constructs for increasing efficiency of transformation of thermophilic host cells for production of carbon-based products of interest and methods for producing carbon- based products of interest are provided.
C12P 1/00 - Préparation de composés ou de compositions, non prévue dans les groupes , utilisant des micro-organismes ou des enzymesProcédés généraux de préparation de composés ou de compositions utilisant des micro-organismes ou des enzymes
The present invention provides solar energy gathering systems, apparatuses and solar gathering methods that capture a greater portion of the energy of an incident spectrum of electromagnetic radiation, typically, solar radiation, than is captured in conventional photobioreactors while allowing for the prevention of deleterious effects caused by certain portions of the incident spectrum of solar radiation.
H01L 31/052 - Moyens de refroidissement directement associés ou intégrés à la cellule PV, p.ex. éléments Peltier intégrés pour refroidissement actif ou puits thermiques directement associés aux cellules PV
34.
BIOSYNTHESIS OF 1-ALKENES IN ENGINEERED MICROORGANISMS
Various 1-alkenes, including 1-nonadecene and 1-octadecene, are synthesized by the engineered microorganisms and methods of the invention. In certain embodiments, the microorganisms comprise recombinant 1-alkene synthases. The engineered microorganisms may be photosynthetic microorganisms such as cyanobacteria.
The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules.
brevets