The present disclosure relates to the methods and compositions for enhancing productivity or yield in fermentation. The methods and compositions provide culturing host cells at a high pH and compositions for culturing host cells at a high pH.
Provided herein are compounds, methods and compositions for determining whether a patient has a neurological disease or disorder is provided, comprising detecting the presence of a detectable target protein, or an accumulated mass thereof, for example, amyloid beta protein or phosphorylated tau protein, or an accumulated mass thereof, in a tissue or a sample of the patient. The detecting may comprise contacting the target protein with a compound described herein.
A61P 25/28 - Médicaments pour le traitement des troubles du système nerveux des troubles dégénératifs du système nerveux central, p. ex. agents nootropes, activateurs de la cognition, médicaments pour traiter la maladie d'Alzheimer ou d'autres formes de démence
Provided herein are methods for a robust production of isoprenoids via one or more biosynthetic pathways. Also provided herein are nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. Also provided herein are fermentation methods for high productivity of isoprenoids from genetically modified host cells.
C12P 23/00 - Préparation de composés contenant un cycle cyclohexène comportant une chaîne latérale non saturée d'au moins dix atomes de carbone liés par des doubles liaisons conjuguées, p. ex. carotènes
C12N 1/32 - Procédés utilisant des alcools saturés inférieurs, c.-à-d. de C1 à C6, ou milieux de culture en contenant
The present disclosure relates to the production of organic compounds by host cell cultures, in particular, organic compounds which are inhibitory to host cells and/or are susceptible to chemical modification, including degradation and/or oxidation, in cell culture conditions. Improved systems and methods are herein disclosed for alleviating inhibition of host cells caused by product accumulation in the culture medium, thereby enhancing cell health and performance, including, for example, increased yield and productivity. Product recovery and purity are furthermore improved.
Provided herein are olefinic feedstocks derived from conjugated hydrocarbon terpenes (e.g., C10-C30 terpenes), methods for making the same, and methods for their use.
C07C 5/03 - Préparation d'hydrocarbures à partir d'hydrocarbures contenant le même nombre d'atomes de carbone par hydrogénation de liaisons doubles carbone-carbone non aromatiques
C10L 1/04 - Combustibles carbonés liquides à base essentielle de mélanges d'hydrocarbures
Provided herein are cosmetic compositions for increasing the integrity of the skin or hair a subject or for reducing irritation of the skin of a subject and methods of using the cosmetic compositions. The cosmetic compositions contain a combination of a occlusive compound and an osmolyte compound. The occlusives of the combinations are squalane, hemisqualane, isosqualane, squalene, or isosqualene. The preferred osmolyte is ectoine. The cosmetics or pharmaceuticals may be used to increase the barrier of the skin or hair, increase the integrity of skin or hair, reduce inflammation of the skin, or prevent aging of the skin of a subject.
A61K 8/49 - Cosmétiques ou préparations similaires pour la toilette caractérisés par la composition contenant des composés organiques contenant des composés hétérocycliques
C12P 17/06 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle contenant un hétérocycle à six chaînons, p. ex. fluorescéine
C12N 15/80 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons
Provided herein are host cells capable of producing a human milk oligosaccharide (HMO), such as yeast cells that are deficient in expression or activity of an endogenous oxidoreductase. Also provided are fermentation compositions including the disclosed host cells, as well as related methods of producing and recovering HMOs generated by the host cells.
C12P 19/00 - Préparation de composés contenant des radicaux saccharide
A23L 33/00 - Modification de la qualité nutritive des alimentsProduits diététiquesLeur préparation ou leur traitement
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
The compositions and methods of the disclosure can be used to purify a cannabinoid in a host cell, such as a yeast cell, genetically modified to express the enzymes of a cannabinoid biosynthetic pathway. Using the compositions and methods of the disclosure, a fermentation composition may be contacted with an enzymatic composition including a serine protease to purify a cannabinoid.
The present disclosure provides systems and methods for the production and recovery of volatile molecules, such as terpenes, and in particular, monoterpenes. By employing the systems and methods disclosed herein, volatile molecules may be recovered from the liquid contents of a bioreactor as well as the air space or headspace of the bioreactor, significantly improving overall recovery.
The present disclosure features compositions and methods for producing one or more cannabinoids, such as cannabidiolic acid (CBDa), in a host cell, such as a yeast cell, that is genetically modified to express the enzymes of a cannabinoid biosynthetic pathway. Using the compositions and methods of the present invention, the host cell may be genetically modified to express one or more enzymes of a cannabinoid biosynthetic pathway, such as an enzyme having CBDa synthase (CBDaS) activity.
The present disclosure features compositions and methods for producing one or more isoprenoid compounds, such as gamma-ambryl acetate (GAA), in a host cell, such as a yeast cell, that is genetically modified to express the enzymes of an isoprenoid biosynthetic pathway, such as a pathway for making GAA. Using the compositions and methods of the present invention, the host cell may be genetically modified to express one or more enzymes of an isoprenoid biosynthetic pathway, such as an enzyme capable of converting manooloxy to GAA. The host cell may be cultured in a medium, for example, in the presence of an agent that regulates expression of the one or more enzymes. The host cell may be incubated for a time sufficient to allow for production of an isoprenoid compound, such as GAA. The isoprenoid compound may then be separated from the host cell or from the medium.
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
N-HETEROCYCLYL SUBSTITUTED 2-CYANO-3-(NAPHTHALEN-2-YL)ACRYLAMIDE DERIVATIVES AS FLUOROPHORS FOR DETECTION OF AMYLOID AND AMYLOID-LIKE PROTEINS FOR DIAGNOSIS OF NEURODEGENERATIVE DISORDERS
Provided herein is the design and synthesis of novel molecular rotor fluorophores of formula I useful for detection of amyloid or amyloid like proteins. The fluorophores are designed to exhibit enhanced fluorescence emission upon associating with amyloid or amyloid like proteins as compared to unbound compound. Also disclosed herein are the compounds for use in methods for diagnosis and treatment of diseases associated with an amyloid or amyloid like proteins, such as e.g. Alzheimer's disease or traumatic brain injury (TBI), Parkinson's disease, vascular dementia, amyotrophic lateral sclerosis, Down syndrome, traumatic brain injury, chronic traumatic encephalopathy, schizophrenia or depression. Exemplary compounds are e.g. N-heterocyclyl substituted 2-cyano-3-(naphthalen-2-yl)acrylamide derivatives, such as e.g. (E)-2-cyano-N-(2-(2-hydroxy)ethoxy)ethyl)-3-(6-(pyrrolidin-1-yl)naphthalen-2-yl) acrylamide (example 1): Experimental data on in-vitro binding studies with amyloid beta is provided.
Provided herein is the design and synthesis of novel molecular rotor fluorophores of formula I useful for detection of amyloid or amyloid like proteins. The fluorophores are designed to exhibit enhanced fluorescence emission upon associating with amyloid or amyloid like proteins as compared to unbound compound. Also disclosed herein are the compounds for use in methods for diagnosis and treatment of diseases associated with an amyloid or amyloid like proteins, such as e.g. Alzheimer's disease or traumatic brain injury (TBI), Parkinson's disease, vascular dementia, amyotrophic lateral sclerosis, Down syndrome, traumatic brain injury, chronic traumatic encephalopathy, schizophrenia or depression. Exemplary compounds are e.g. N-heterocyclyl substituted 2-cyano-3-(naphthalen-2-yl)acrylamide derivatives, such as e.g. (E)-2-cyano-N-(2-(2-hydroxy)ethoxy)ethyl)-3-(6-(pyrrolidin-1-yl)naphthalen-2-yl) acrylamide (example 1): Experimental data on in-vitro binding studies with amyloid beta is provided.
C07D 203/10 - Radicaux substitués par des atomes d'oxygène liés par des liaisons simples
C07D 203/14 - Composés hétérocycliques contenant des cycles à trois chaînons ne comportant qu'un seul atome d'azote comme unique hétéro-atome du cycle non condensés avec d'autres cycles ne comportant pas de liaison double entre chaînons cycliques ou entre chaînons cycliques et chaînons non cycliques avec uniquement des atomes d'hydrogène, des radicaux hydrocarbonés ou des radicaux hydrocarbonés substitués, liés directement à l'atome d'azote du cycle avec des carbocycles liés directement à l'atome d'azote du cycle
C07D 205/04 - Composés hétérocycliques comportant des cycles à quatre chaînons ne contenant qu'un atome d'azote comme unique hétéro-atome du cycle non condensés avec d'autres cycles ne comportant pas de liaison double entre chaînons cycliques ou entre chaînons cycliques et chaînons non cycliques
C07D 207/06 - Composés hétérocycliques contenant des cycles à cinq chaînons, non condensés avec d'autres cycles, ne comportant qu'un atome d'azote comme unique hétéro-atome du cycle avec uniquement des atomes d'hydrogène ou de carbone liés directement à l'atome d'azote du cycle ne comportant pas de liaison double entre chaînons cycliques ou entre chaînons cycliques et chaînons non cycliques avec des radicaux contenant uniquement des atomes d'hydrogène et de carbone, liés aux atomes de carbone du cycle
C07D 211/14 - Composés hétérocycliques contenant des cycles pyridiques hydrogénés, non condensés avec d'autres cycles avec uniquement des atomes d'hydrogène et de carbone liés directement à l'atome d'azote du cycle ne comportant pas de liaison double entre chaînons cycliques ou entre chaînons cycliques et chaînons non cycliques avec des radicaux hydrocarbonés ou des radicaux hydrocarbonés substitués, liés directement aux atomes de carbone du cycle avec des radicaux ne contenant que des atomes de carbone et d'hydrogène liés aux atomes de carbone du cycle avec des radicaux hydrocarbonés ou des radicaux hydrocarbonés substitués, liés à l'atome d'azote du cycle
The present disclosure features compositions and methods for producing one or more isoprenoid compounds, such as E-copalol, in a host cell, such as a yeast cell, that is genetically modified to express the enzymes of an isoprenoid biosynthetic pathway, such as a pathway for making E-copalol. Using the compositions and methods of the present invention, the host cell may be genetically modified to express one or more enzymes of an isoprenoid biosynthetic pathway, such as a copalyl-diphosphate (CPP) pyrophosphatase. The host cell may then be cultured in a medium, for example, in the presence of an agent that regulates expression of the one or more enzymes. The host cell may further be incubated for a time sufficient to allow for production of an isoprenoid compound, such as E-copalol, by the host cell. The isoprenoid compound may then be separated from the host cell or from the medium.
The present invention provides compounds derived from farnesene and methods of producing the compounds from farnesene. In addition, compositions and methods of using the compositions as personal care products and other consumer products are provided, wherein the compositions comprise compounds derived from farnesene.
C07D 307/33 - Atomes d'oxygène en position 2, l'atome d'oxygène étant sous la forme céto ou énol non substituée
C07C 69/003 - Esters d'alcools saturés dont le groupe hydroxyle estérifié est lié à un atome de carbone acyclique
C07C 69/22 - Esters d'acides acycliques monocarboxyliques saturés dont le groupe carboxyle est lié à un atome de carbone acyclique ou à l'hydrogène avec au moins trois atomes de carbone dans la partie acide
A61Q 19/00 - Préparations pour les soins de la peau
A61K 31/341 - Composés hétérocycliques ayant l'oxygène comme seul hétéro-atome d'un cycle, p. ex. fungichromine ayant des cycles à cinq chaînons avec un oxygène comme seul hétéro-atome d'un cycle, p. ex. isosorbide non condensés avec un autre cycle, p. ex. ranitidine, furosémide, bufétolol, muscarine
16.
COMPOSITIONS AND METHODS FOR IMPROVED PRODUCTION OF HUMAN MILK OLIGOSACCHARIDES
Provided herein are host cells capable of producing a human milk oligosaccharide (HMO) or HMO precursor, such as yeast cells that include one or more heterologous nucleic acids encoding one or more enzymes of an HMO biosynthetic pathway, such as a sialic acid synthase, a UDP-N-acetylglucosamine 2-epimerase, a β-galactoside-α-2,6-sialyltransferase, and/or a CMP-Neu5Ac synthetase. Also provided are fermentation compositions including the disclosed host cells, as well as related methods of producing and recovering HMOs or HMO precursors generated by the host cells.
Provided herein are recombinant host cells, compositions, and methods for the production of retinol, retinal, beta-carotene, lycopene, or phytoene (retinol or retinol precursor). The host cells are genetically modified to contain heterologous nucleic acids that express novel enzymes that enable the host cell to produce the retinol or retinol precursor from a carbon source such as sucrose. The host cells, compositions, and methods disclosed herein provide an efficient route for the heterologous production of retinol, retinal, beta-carotene, lycopene, or phytoene.
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/08 - Oxydoréductases (1.), p. ex. luciférase agissant sur le peroxyde d'hydrogène comme accepteur (1.11)
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12P 23/00 - Préparation de composés contenant un cycle cyclohexène comportant une chaîne latérale non saturée d'au moins dix atomes de carbone liés par des doubles liaisons conjuguées, p. ex. carotènes
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12P 23/00 - Préparation de composés contenant un cycle cyclohexène comportant une chaîne latérale non saturée d'au moins dix atomes de carbone liés par des doubles liaisons conjuguées, p. ex. carotènes
Provided herein are recombinant host cells, compositions, and methods for the production of the sesquiterpenoids: alpha-humulene, beta-caryophyllene, valencene, delta-cadinene, delta-guaiene, germacrene A, germacrene D, beta- cubebene, valerena-4,7(11)-diene, nerolidol, and bisabolol. The host cells are genetically modified to contain heterologous nucleic acids that express novel enzymes that enable the host cell to produce the sesquiterpenoids from a carbon source such as sucrose. The host cells, compositions, and methods disclosed herein provide an efficient route for the heterologous production of alpha-humulene, beta-caryophyllene, valencene, delta-cadinene, delta-guaiene, germacrene A, germacrene D, beta-cubebene, valerena-4,7(11)-diene, nerolidol, or bisabolol.
C07K 14/415 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de végétaux
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
20.
AMYLOID TARGETING AGENTS AND METHODS OF USING THE SAME
Provided herein is the design and synthesis of novel molecular rotor fluorophores useful for detection of amyloid or amyloid like proteins. The fluorophores are designed to exhibit enhanced fluorescence emission upon associating with amyloid or amyloid like proteins as compared to unbound compound. Also disclosed herein are methods for treating diseases associated with amyloid or amyloid like proteins.
C07D 295/155 - Composés hétérocycliques contenant des cycles polyméthylène imine d'au moins cinq chaînons, des cycles aza-3 bicyclo [3.2.2] nonane, piperazine, morpholine ou thiomorpholine, ne comportant que des atomes d'hydrogène liés directement aux atomes de carbone du cycle avec des radicaux hydrocarbonés substitués liés aux atomes d'azote du cycle substitués par des atomes de carbone comportant trois liaisons à des hétéro-atomes avec au plus une liaison à un halogène, p. ex. radicaux ester ou nitrile avec les atomes d'azote du cycle et les atomes de carbone comportant trois liaisons à des hétéro-atomes séparés par des carbocycles ou par des chaînes carbonées interrompues par des carbocycles
A61B 5/00 - Mesure servant à établir un diagnostic Identification des individus
A61P 25/28 - Médicaments pour le traitement des troubles du système nerveux des troubles dégénératifs du système nerveux central, p. ex. agents nootropes, activateurs de la cognition, médicaments pour traiter la maladie d'Alzheimer ou d'autres formes de démence
C07C 255/42 - Nitriles d'acides carboxyliques ayant des groupes cyano liés à des atomes de carbone acycliques ayant des groupes cyano liés à des atomes de carbone acycliques d'un squelette carboné contenant au moins un cycle aromatique à six chaînons le squelette carboné étant substitué de plus par des atomes d'azote liés par des liaisons simples et n'étant pas liés de plus à d'autres hétéro-atomes
C07C 255/58 - Nitriles d'acides carboxyliques ayant des groupes cyano liés à des atomes de carbone de cycles aromatiques à six chaînons d'un squelette carboné contenant des groupes cyano et des atomes d'azote, liés par des liaisons simples et n'étant pas liés de plus à d'autres hétéro-atomes, liés au squelette carboné
C07C 255/59 - Nitriles d'acides carboxyliques ayant des groupes cyano liés à des atomes de carbone de cycles aromatiques à six chaînons d'un squelette carboné contenant des groupes cyano et des atomes d'azote, liés par des liaisons simples et n'étant pas liés de plus à d'autres hétéro-atomes, liés au squelette carboné le squelette carboné étant substitué de plus par des atomes d'oxygène liés par des liaisons simples
C07C 311/35 - Sulfonamides, le squelette carboné de la partie acide étant substitué de plus par des atomes d'azote liés par des liaisons simples, ne faisant pas partie de groupes nitro ou nitroso ayant les atomes de soufre des groupes sulfonamide liés à des atomes de carbone acycliques d'un squelette carboné non saturé contenant des cycles
C07D 211/06 - Composés hétérocycliques contenant des cycles pyridiques hydrogénés, non condensés avec d'autres cycles avec uniquement des atomes d'hydrogène et de carbone liés directement à l'atome d'azote du cycle ne comportant pas de liaison double entre chaînons cycliques ou entre chaînons cycliques et chaînons non cycliques
C07D 241/04 - Composés hétérocycliques contenant des cycles diazine-1,4 ou diazine-1,4 hydrogéné non condensés avec d'autres cycles ne comportant pas de liaisons doubles entre chaînons cycliques ou entre chaînons cycliques et chaînons non cycliques
C07D 265/30 - Oxazines-1, 4Oxazines-1, 4 hydrogénées non condensées avec d'autres cycles
C07D 295/04 - Composés hétérocycliques contenant des cycles polyméthylène imine d'au moins cinq chaînons, des cycles aza-3 bicyclo [3.2.2] nonane, piperazine, morpholine ou thiomorpholine, ne comportant que des atomes d'hydrogène liés directement aux atomes de carbone du cycle avec des radicaux hydrocarbonés substitués liés aux atomes d'azote du cycle
C07D 307/52 - Radicaux substitués par des atomes d'azote ne faisant par partie d'un radical nitro
C07D 333/22 - Radicaux substitués par des hétéro-atomes liés par une liaison double ou par deux hétéro-atomes, autres que des halogènes, liés au même atome de carbone par des liaisons simples
C07D 401/04 - Composés hétérocycliques contenant plusieurs hétérocycles comportant des atomes d'azote comme uniques hétéro-atomes du cycle, au moins un cycle étant un cycle à six chaînons avec un unique atome d'azote contenant deux hétérocycles liés par une liaison directe de chaînon cyclique à chaînon cyclique
C07D 401/14 - Composés hétérocycliques contenant plusieurs hétérocycles comportant des atomes d'azote comme uniques hétéro-atomes du cycle, au moins un cycle étant un cycle à six chaînons avec un unique atome d'azote contenant au moins trois hétérocycles
C07D 405/04 - Composés hétérocycliques contenant à la fois un ou plusieurs hétérocycles comportant des atomes d'oxygène comme uniques hétéro-atomes du cycle et un ou plusieurs hétérocycles comportant des atomes d'azote comme uniques hétéro-atomes du cycle contenant deux hétérocycles liés par une liaison directe de chaînon cyclique à chaînon cyclique
C07D 405/10 - Composés hétérocycliques contenant à la fois un ou plusieurs hétérocycles comportant des atomes d'oxygène comme uniques hétéro-atomes du cycle et un ou plusieurs hétérocycles comportant des atomes d'azote comme uniques hétéro-atomes du cycle contenant deux hétérocycles liés par une chaîne carbonée contenant des cycles aromatiques
C07D 407/10 - Composés hétérocycliques contenant plusieurs hétérocycles, au moins un cycle comportant des atomes d'oxygène comme uniques hétéro-atomes du cycle, non prévus par le groupe contenant deux hétérocycles liés par une chaîne carbonée contenant des cycles aromatiques
C07D 409/04 - Composés hétérocycliques contenant plusieurs hétérocycles, au moins un cycle comportant des atomes de soufre comme uniques hétéro-atomes du cycle contenant deux hétérocycles liés par une liaison directe de chaînon cyclique à chaînon cyclique
C07D 409/10 - Composés hétérocycliques contenant plusieurs hétérocycles, au moins un cycle comportant des atomes de soufre comme uniques hétéro-atomes du cycle contenant deux hétérocycles liés par une chaîne carbonée contenant des cycles aromatiques
C07D 409/14 - Composés hétérocycliques contenant plusieurs hétérocycles, au moins un cycle comportant des atomes de soufre comme uniques hétéro-atomes du cycle contenant au moins trois hétérocycles
C07D 413/10 - Composés hétérocycliques contenant plusieurs hétérocycles, au moins un cycle comportant des atomes d'azote et d'oxygène comme uniques hétéro-atomes du cycle contenant deux hétérocycles liés par une chaîne carbonée contenant des cycles aromatiques
C07D 413/12 - Composés hétérocycliques contenant plusieurs hétérocycles, au moins un cycle comportant des atomes d'azote et d'oxygène comme uniques hétéro-atomes du cycle contenant deux hétérocycles liés par une chaîne contenant des hétéro-atomes comme chaînons
C07H 13/04 - Composés contenant des radicaux saccharide estérifiés soit par l'acide carbonique ou ses dérivés, soit par des acides organiques, p. ex. acides phosphoniques par des acides carboxyliques comportant les radicaux carboxyle estérifiants liés à des atomes de carbone acycliques
Provided herein methods of producing and isolating a cannabinoid from a fermentation composition including are host cells capable of producing a cannabinoid such by combining a mixture including a cannabigerolic acid with a cannabichromenic acid (CBCa) synthase, optionally in an oil mixture. Also provided herein are CBCa synthases having improved activity in comparison to the wild-type enzyme.
Provided herein are hair care compositions containing a sugar cane derived alkanes or squalane and which are generally free from silicones. The hair care compositions provided have beneficial properties including UV and thermal protection as well as restorative and protective properties. Also provided are methods of restoring and protecting hair from damage caused by frequent styling.
Provided herein are genetically modified host cells. compositions. and methods for improved production of vanillin and/or glucovanillin. The host cells. compositions. and methods described herein provide an efficient route for the heterologous production of vanillin and/or glucovanillin and any compound that can be synthesized or biosynthesized from either or both.
Provided herein are methods and compositions for determining whether a patient suffers from systemic amyloidosis, comprising detecting the presence of a misfolded or aggregated protein in a tissue of the patient, wherein the detecting comprises contacting the misfolded or aggregated transthyretin protein with a compound described herein. In some embodiments, the misfolded or aggregated protein is transthyretin.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
The disclosure provides cosmetic compositions including cannabigerol (CBG), as well as methods of using the same for reducing skin redness, improving skin barrier functionality, brightening the skin of a subject, and enhancing the outward appearance of the skin of a subject (e.g., a male or female human subject).
Provided herein are methods of isolating a retinoid that includes: providing a fermentation broth comprising the retinoid and an overlay; performing a liquid-solid and liquid-liquid separation on the fermentation broth to produce a crude oil and a waste heavy phase; mixing the crude oil with a solvent extraction system to extract the retinoid from the crude oil; and stripping the solvent extraction system from the extracted retinoid to thereby isolate the retinoid. Also provided herein are methods of isolating a retinoid from a composition including the retinoid and farnesol that include: providing a composition including the retinoid and farnesol; feeding the composition into a chromatography system; and collecting the retinoid, thereby isolating the retinoid.
C07C 403/08 - Dérivés du cyclohexane ou d'un cyclohexène, comportant une chaîne latérale avec une partie non saturée d'au moins quatre atomes de carbone en ligne, ladite partie étant directement attachée aux cycles cyclohexane ou cyclohexène, p. ex. vitamine A, bêta-carotène, bêta-ionone ayant des chaînes latérales substituées par des atomes d'oxygène liés par des liaisons simples par des groupes hydroxy
28.
COMPOSITIONS AND METHODS FOR PRODUCTION OF MYRCENE
Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyroplosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.
Provided herein are variant β-1,3-N-acetylglucosaminyltransferase polypeptides capable of producing lacto-n-neotetraose, yeast cells capable of producing one or more human milk oligosaccharides, and methods of making such cells. Also, provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering HMOs generated by the yeast cells.
C12P 19/18 - Préparation de composés contenant des radicaux saccharide préparés par action d'une transférase glycosylique, p. ex. alpha-, bêta- ou gamma-cyclodextrines
C07C 5/03 - Préparation d'hydrocarbures à partir d'hydrocarbures contenant le même nombre d'atomes de carbone par hydrogénation de liaisons doubles carbone-carbone non aromatiques
C10L 1/04 - Combustibles carbonés liquides à base essentielle de mélanges d'hydrocarbures
Provided herein are compounds, methods and compositions for determining whether a patient has a neurological disease or disorder is provided, comprising detecting the presence of a detectable target protein, or an accumulated mass thereof, for example, amyloid beta protein or phosphorylated tau protein, or an accumulated mass thereof, in a tissue or a sample of the patient. The detecting may comprise contacting the target protein with a compound described herein.
A61P 25/28 - Médicaments pour le traitement des troubles du système nerveux des troubles dégénératifs du système nerveux central, p. ex. agents nootropes, activateurs de la cognition, médicaments pour traiter la maladie d'Alzheimer ou d'autres formes de démence
Provided herein are methods for a robust production of isoprenoids via one or more biosynthetic pathways. Also provided herein are nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. Also provided herein are fermentation methods for high productivity of isoprenoids from genetically modified host cells.
C12P 23/00 - Préparation de composés contenant un cycle cyclohexène comportant une chaîne latérale non saturée d'au moins dix atomes de carbone liés par des doubles liaisons conjuguées, p. ex. carotènes
C12N 1/32 - Procédés utilisant des alcools saturés inférieurs, c.-à-d. de C1 à C6, ou milieux de culture en contenant
Provided herein are fermentation compositions and methods for improved production of vanillin and/or glucovanillin. The compositions and methods described herein provide efficient routes for the production of vanillin and/or glucovanillin and any compound that can be synthesized or biosynthesized from either or both.
The present disclosure provides compositions and methods for producing high-purity squalene from a squalene source, for example from host cells, such as yeast cells that are capable of synthesizing squalene. Further provided herein are compositions containing high-purity squalene, as well as methods of using the same in pharmaceutical formulations, such as in adjuvant formulations for use in vaccines.
C07C 7/10 - Purification, séparation ou stabilisation d'hydrocarburesEmploi d'additifs par extraction, c.-à-d. purification ou séparation d'hydrocarbures liquides à l'aide de liquides
The present disclosure relates to the methods and compositions for enhancing productivity or yield in fermentation. The methods include culturing host cells at a high pH to increase oxygen uptake as well as a higher yield and productivity of the compositions produced using the methods.
The present disclosure provides compositions and methods for producing a cell culture product in a cell culture composition, particularly for cell culture product s that impair the growth and/or function of the host cells in which they are biosynthesized, such as isoprenoids or terpenes.
C07C 7/10 - Purification, séparation ou stabilisation d'hydrocarburesEmploi d'additifs par extraction, c.-à-d. purification ou séparation d'hydrocarbures liquides à l'aide de liquides
C12P 1/02 - Préparation de composés ou de compositions, non prévue dans les groupes , utilisant des micro-organismes ou des enzymesProcédés généraux de préparation de composés ou de compositions utilisant des micro-organismes ou des enzymes utilisant des champignons
C12P 1/04 - Préparation de composés ou de compositions, non prévue dans les groupes , utilisant des micro-organismes ou des enzymesProcédés généraux de préparation de composés ou de compositions utilisant des micro-organismes ou des enzymes utilisant des bactéries
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
Provided herein are genetically modified yeast cells capable of producing one or more human milk oligosaccharides (HMOs) and methods of making such cells. The yeast cells are engineered to comprise a heterologous nucleic acid encoding a variant ABC transporter protein YOR1 from Saccharomyces cerevisiae with improved 2'-fucosyllactose export activity and one or more heterologous nucleic acids that encode enzymes of a HMO biosynthetic pathway. Also provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering HMOs generated by the yeast cells.
C12P 19/00 - Préparation de composés contenant des radicaux saccharide
C12N 15/01 - Préparation de mutants sans introduction de matériel génétique étrangerProcédés de criblage à cet effet
C07K 14/395 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de champignons provenant de levures provenant de Saccharomyces
A23L 33/125 - Modification de la qualité nutritive des alimentsProduits diététiquesLeur préparation ou leur traitement en utilisant des additifs contenant des sirops d'hydrate de carboneModification de la qualité nutritive des alimentsProduits diététiquesLeur préparation ou leur traitement en utilisant des additifs contenant des sucresModification de la qualité nutritive des alimentsProduits diététiquesLeur préparation ou leur traitement en utilisant des additifs contenant des alcools de sucreModification de la qualité nutritive des alimentsProduits diététiquesLeur préparation ou leur traitement en utilisant des additifs contenant des hydrolysats d'amidon
A23L 33/00 - Modification de la qualité nutritive des alimentsProduits diététiquesLeur préparation ou leur traitement
38.
COMPOSITIONS AND METHODS FOR IMPROVED PRODUCTION OF STEVIOL GLYCOSIDES
Provided herein are variant uridine-5'-diphosphate glycosyltransferase polypeptides capable of producing steviol glycosides, yeast cells capable of producing steviol glycosides, and methods of making such cells. Also provided are fermentation compositions including the disclosed host cells, and related methods of producing and recovering steviol glycosides generated by the yeast cells.
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 15/81 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons pour levures
C12P 19/18 - Préparation de composés contenant des radicaux saccharide préparés par action d'une transférase glycosylique, p. ex. alpha-, bêta- ou gamma-cyclodextrines
39.
Compounds, compositions, and methods for recovering water-immiscible compounds from microbial biomass
The disclosure provides compounds useful as adjuvants in vaccines, as well as methods of synthesizing such compounds and methods of using such compounds in the formulation of a vaccine. The disclosure also features methods of administering such vaccines to a subject (e.g., a mammalian subject, such as a human) in order to treat or prevent one or more diseases, such as a disease caused by a viral or bacterial infection.
A61K 39/39 - Préparations médicinales contenant des antigènes ou des anticorps caractérisées par les additifs immunostimulants, p. ex. par les adjuvants chimiques
C07C 43/15 - Éthers non saturés ne contenant que des liaisons doubles carbone-carbone non aromatiques
C07C 43/215 - Éthers une liaison sur l'oxygène de la fonction éther étant sur un atome de carbone d'un cycle aromatique à six chaînons avec une insaturation autre que celle des cycles aromatiques à six chaînons
C07C 49/258 - Composés non saturés comportant des groupes cétone liés à des atomes de carbone acycliques contenant des groupes —CHO
The compositions and methods of the disclosure can be used to produce a cannabinoid in a host cell, such as a yeast cell. For example, the disclosure features host cells (e.g., yeast cells) modified to express one or more enzymes of a cannabinoid biosynthetic pathway, such as an acyl activating enzyme (AAE), a tetraketide synthase (TKS), a cannabigerolic acid synthase (CBGaS), and/or an olivetolic acid cyclase (OAC).
Provided herein are sunscreen formulations containing a metal oxide sunscreen, squalane, and an antioxidant. The sunscreen formulations provided have increased SPF per unit mass of the metal oxide compared to sunscreen formulations that contain metal oxide without the addition of squalane and antioxidant. Also provided are methods of preventing UV damages to skin involving the application of the sunscreen formulation.
A method includes causing a graphical user interface to be output to a display. The method also includes detecting a triggering of a purchase icon by a first user and causing a transaction to occur. The transaction is a commitment by the first user to spend a minimum amount of currency on one or more products or services being offered by a presenter. The method further includes detecting a triggering of a sharing icon to cause information to be shared with a second user. The information includes one or more selectable links configured to facilitate one or more of accessing of a video or purchasing the one or more products or services being offered. The method additionally includes monitoring data indicative of how the second user interacts with the information and linking the data indicative of how the second user interacts with the information to the first user.
G06Q 30/0214 - Systèmes de récompense de recommandation
G06F 3/04817 - Techniques d’interaction fondées sur les interfaces utilisateur graphiques [GUI] fondées sur des propriétés spécifiques de l’objet d’interaction affiché ou sur un environnement basé sur les métaphores, p. ex. interaction avec des éléments du bureau telles les fenêtres ou les icônes, ou avec l’aide d’un curseur changeant de comportement ou d’aspect utilisant des icônes
G06Q 30/0207 - Remises ou incitations, p. ex. coupons ou rabais
G06Q 50/00 - Technologies de l’information et de la communication [TIC] spécialement adaptées à la mise en œuvre des procédés d’affaires d’un secteur particulier d’activité économique, p. ex. aux services d’utilité publique ou au tourisme
44.
COMPOSITIONS AND METHODS FOR IMPROVED PRODUCTION OF HUMAN MILK OLIGOSACCHARIDES
Provided herein are host cells capable of producing a human milk oligosaccharide (HMO), such as yeast cells that include one or more heterologous nucleic acids encoding one or more enzymes of the HMO biosynthetic pathway, such as a fucosyltransferase, GDP-mannose dehydratase, lactose permease, and/or fucose synthase. Also provided are fermentation compositions including the disclosed host cells, as well as related methods of producing and recovering HMOs generated by the host cells.
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 15/64 - Méthodes générales pour la préparation du vecteur, pour son introduction dans la cellule ou pour la sélection de l'hôte contenant le vecteur
C12N 1/15 - ChampignonsLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12P 19/00 - Préparation de composés contenant des radicaux saccharide
Provided herein are sunscreen formulations containing a metal oxide sunscreen, squalane, and an antioxidant. The sunscreen formulations provided have increased SPF per unit mass of the metal oxide compared to sunscreen formulations that contain metal oxide without the addition of squalane and antioxidant. Also provided are methods of preventing UV damages to skin involving the application of the sunscreen formulation.
Provided herein are host cells capable of producing a human milk oligosaccharide (HMO), such as yeast cells that are deficient in expression or activity of an endogenous oxidoreductase. Also provided are fermentation compositions including the disclosed host cells, as well as related methods of producing and recovering HMOs generated by the host cells.
Provided herein antiperspirant composition formulations that contain hemisqualane and lack organosilicon compounds. The hemisqualane provides the carrier and emollient activities that are typically supplied by an organosilicon compound. The antiperspirant formulations provided herein have a benefit of not releasing organosilicon compounds into the environment during and after their use.
Provided herein are genetically modified host cells, compositions, and methods for improved production of artemisinic acid. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of amorpha-4,11-diene 12-monooxygenase. Also provided herein are methods for screening for variants of cytochrome p450 enzymes that have increased enzymatic activity relative to a parental control enzyme.
N-HETEROCYCLYL SUBSTITUTED 2-CYANO-3-(NAPHTHALEN-2-YL)ACRYLAMIDE DERIVATIVES AS FLUOROPHORS FOR DETECTION OF AMYLOID AND AMYLOID-LIKE PROTEINS FOR DIAGNOSIS OF NEURODEGENERATIVE DISORDERS
Provided herein is the design and synthesis of novel molecular rotor fluorophores of formula I useful for detection of amyloid or amyloid like proteins. The fluorophores are designed to exhibit enhanced fluorescence emission upon associating with amyloid or amyloid like proteins as compared to unbound compound. Also disclosed herein are the compounds for use in methods for diagnosis and treatment of diseases associated with an amyloid or amyloid like proteins, such as e.g. Alzheimer's disease or traumatic brain injury (TBI), Parkinson's disease, vascular dementia, amyotrophic lateral sclerosis, Down syndrome, traumatic brain injury, chronic traumatic encephalopathy, schizophrenia or depression. Exemplary compounds are e.g. N-heterocyclyl substituted 2-cyano-3- (naphthalen-2-yl)acrylamide derivatives, such as e.g. (E)-2-cyano- N-(2-(2-hydroxy)ethoxy)ethyl)-3-(6-(pyrrolidin-1-yl)naphthalen-2-yl) acrylamide (example 1): Experimental data on in-vitro binding studies with amyloid beta is provided.
A61K 31/396 - Composés hétérocycliques ayant l'azote comme hétéro-atome d'un cycle, p. ex. guanéthidine ou rifamycines ayant des cycles à trois chaînons, p. ex. aziridine
A61K 31/397 - Composés hétérocycliques ayant l'azote comme hétéro-atome d'un cycle, p. ex. guanéthidine ou rifamycines ayant des cycles à quatre chaînons, p. ex. azétidine
A61K 31/402 - Composés hétérocycliques ayant l'azote comme hétéro-atome d'un cycle, p. ex. guanéthidine ou rifamycines ayant des cycles à cinq chaînons avec un azote comme seul hétéro-atome d'un cycle, p. ex. sulpiride, succinimide, tolmétine, buflomédil substitués par un groupe aryle en position 1, p. ex. pirétanide
A61K 31/495 - Composés hétérocycliques ayant l'azote comme hétéro-atome d'un cycle, p. ex. guanéthidine ou rifamycines ayant des cycles à six chaînons avec deux azote comme seuls hétéro-atomes d'un cycle, p. ex. pipérazine
A61P 25/28 - Médicaments pour le traitement des troubles du système nerveux des troubles dégénératifs du système nerveux central, p. ex. agents nootropes, activateurs de la cognition, médicaments pour traiter la maladie d'Alzheimer ou d'autres formes de démence
C07D 295/14 - Composés hétérocycliques contenant des cycles polyméthylène imine d'au moins cinq chaînons, des cycles aza-3 bicyclo [3.2.2] nonane, piperazine, morpholine ou thiomorpholine, ne comportant que des atomes d'hydrogène liés directement aux atomes de carbone du cycle avec des radicaux hydrocarbonés substitués liés aux atomes d'azote du cycle substitués par des atomes de carbone comportant trois liaisons à des hétéro-atomes avec au plus une liaison à un halogène, p. ex. radicaux ester ou nitrile
The present disclosure features compositions and methods for producing one or more cannabinoids, such as cannabidiolic acid (CBDa), in a host cell, such as a yeast cell, that is genetically modified to express the enzymes of a cannabinoid biosynthetic pathway. Using the compositions and methods of the present invention, the host cell may be genetically modified to express one or more enzymes of a cannabinoid biosynthetic pathway, such as an enzyme having CBDa synthase (CBDaS) activity.
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
The present disclosure features compositions and methods for producing one or more cannabinoids, such as cannabidiolic acid (CBDa) or cannabidiol (CBD), in a host cell, such as a yeast cell, that is genetically modified to express the enzymes of a cannabinoid biosynthetic pathway. Using the compositions and methods of the present invention, the host cell may be genetically modified to express one or more enzymes of a cannabinoid biosynthetic pathway, such as CBDaS. The host cell may be cultured in the medium in the presence of a first cannabinoid, for example CBGa, and incubated for a time sufficient to allow for bioconversion of the first cannabinoid to a second cannabinoid, for example CBDa, by the host cell.
The present disclosure features compositions and methods for producing one or more isoprenoid compounds, such as gamma-ambryl acetate (GAA), in a host cell, such as a yeast cell, that is genetically modified to express the enzymes of an isoprenoid biosynthetic pathway, such as a pathway for making GAA. Using the compositions and methods of the present invention, the host cell may be genetically modified to express one or more enzymes of an isoprenoid biosynthetic pathway, such as an enzyme capable of converting manooloxy to GAA. The host cell may be cultured in a medium, for example, in the presence of an agent that regulates expression of the one or more enzymes. The host cell may be incubated for a time sufficient to allow for production of an isoprenoid compound, such as GAA. The isoprenoid compound may then be separated from the host cell or from the medium.
The present disclosure features compositions and methods for producing one or more isoprenoid compounds, such as E-copalol, in a host cell, such as a yeast cell, that is genetically modified to express the enzymes of an isoprenoid biosynthetic pathway, such as a pathway for making E-copalol. Using the compositions and methods of the present invention, the host cell may be genetically modified to express one or more enzymes of an isoprenoid biosynthetic pathway, such as a copalyl-diphosphate (CPP) pyrophosphatase. The host cell may then be cultured in a medium, for example, in the presence of an agent that regulates expression of the one or more enzymes. The host cell may further be incubated for a time sufficient to allow for production of an isoprenoid compound, such as E-copalol, by the host cell. The isoprenoid compound may then be separated from the host cell or from the medium.
The compositions and methods of the disclosure can be used to purify a cannabinoid in a host cell, such as a yeast cell, genetically modified to express the enzymes of a cannabinoid biosynthetic pathway. Using the compositions and methods of the disclosure, a fermentation composition may be contacted with an enzymatic composition including a serine protease to purify a cannabinoid.
The present disclosure features compositions and methods for producing one or more cannabinoids in a host cell that is genetically modified to express the enzymes of a cannabinoid biosynthetic pathway. Using the compositions and methods of the disclosure, a cannabinoid-producing host cell may be contacted with an oil so as to enhance the recovery of the cannabinoid from the host cell.
Provided herein are methods and compositions for determining whether a patient suffers from a neurological disease or disorder is provided, comprising detecting the presence of a phosphorylated tau protein in a tissue of the patient, wherein the detecting comprises contacting the phosphorylated tau protein with a compound described herein.
A61P 25/28 - Médicaments pour le traitement des troubles du système nerveux des troubles dégénératifs du système nerveux central, p. ex. agents nootropes, activateurs de la cognition, médicaments pour traiter la maladie d'Alzheimer ou d'autres formes de démence
57.
COMPOSITIONS AND METHODS FOR DELIVERING CANNABINOIDS TO SKIN
Provided herein are compositions for delivering one or more cannabinoids to the skin of a subject and methods of using the compositions to deliver the one or more cannabinoids to the skin of a subject. The compositions contain a carrier that facilitates the delivery and permeation of cannabinoids to and through the skin. The carriers of the compositions contain squalane or mixtures of squalane and hemisqualane. The compositions described herein may serve as the base for the preparation of various cosmetics or various pharmaceuticals. The cosmetics or pharmaceuticals may be used to reduce inflammation, control pain, or prevent aging of the skin of a subject.
A61K 31/352 - Composés hétérocycliques ayant l'oxygène comme seul hétéro-atome d'un cycle, p. ex. fungichromine ayant des cycles à six chaînons avec un oxygène comme seul hétéro-atome d'un cycle condensés avec des carbocycles, p. ex. cannabinols, méthanthéline
Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. In some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.
C12N 15/90 - Introduction stable d'ADN étranger dans le chromosome
C12Q 1/6897 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques faisant intervenir des gènes rapporteurs liés de façon fonctionnelle à des promoteurs
C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
Provided herein are compositions and methods for uncoupling the yield and productivity of an isoprenoid compound produced in a host cell. In some embodiments, the yield and productivity are uncoupled by genetically modifying the host cell to reduce flux through the citric acid cycle (TCA). In other embodiments, the yield and productivity are uncoupled by reducing the levels of ATP in the host cell.
Provided herein are genetically modified yeast cells capable of producing one or more human milk oligosaccharides (HMOs) and methods of making such cells. The yeast cells are engineered to comprise a heterologous nucleic acid encoding a transporter protein and one or more heterologous nucleic acids that encode enzymes of a HMO biosynthetic pathway. Also provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering HMOs generated by the yeast cells.
Provided herein are genetically modified host cells, compositions, and methods for improved production of vanillin and/or glucovanillin. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of vanillin and/or glucovanillin and any compound that can be synthesized or biosynthesized from either or both.
C12P 7/24 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carbonyle
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 15/81 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons pour levures
63.
METHODS OF DETECTING SYSTEMIC AMYLOIDOSIS VIA BINDING TO MISFOLDED OR AGGREGATED PROTEIN
Provided herein are methods and compositions for determining whether a patient suffers from systemic amyloidosis, comprising detecting the presence of a misfolded or aggregated protein in a tissue of the patient, wherein the detecting comprises contacting the misfolded or aggregated transthyretin protein with a compound described herein. In some embodiments, the misfolded or aggregated protein is transthyretin.
C07C 15/24 - Hydrocarbures polycycliques condensés contenant deux cycles
C07C 255/01 - Nitriles d'acides carboxyliques ayant des groupes cyano liés à des atomes de carbone acycliques
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
64.
MODIFIED HOST CELLS FOR HIGH EFFICIENCY PRODUCTION OF VANILLIN
Provided herein are genetically modified host cells, compositions, and methods for improved production of vanillin and/or glucovanillin. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of vanillin and/or glucovanillin and any compound that can be synthesized or biosynthesized from either or both.
Provided herein are genetically modified host cells, compositions, and methods for improved production of steviol glycosides. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of Ro.KAH. The host cell further contains one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of steviol glycosides including rebaudioside A, rebaudioside D, and rebaudioside M.
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12P 19/56 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un système cyclique condensé d'au moins trois carbocycles, p. ex. daunomycine, adriamycine
66.
Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
Provided herein are compositions and methods of integrating one or more exogenous donor nucleic acids into one or more exogenous landing pads engineered into a host cell's genome. In certain embodiments, the exogenous landing pads and exogenous donor nucleic acids comprise standardized, compatible homology regions so that exogenous donor nucleic acids can integrate into any of the landing pads, independent of the genomic sequences surrounding the landing pads. In certain embodiments, the methods comprise contacting the host cell comprising landing pads with one or more exogenous donor nucleic acids, and a nuclease capable of causing a double-strand break within the landing pads, and recovering a host cell comprising one or more exogenous donor nucleic acids integrated in any of the landing pads.
The disclosure provides cosmetic compositions including cannabigerol (CBG), as well as methods of using the same for reducing skin redness, improving skin barrier functionality, brightening the skin of a subject, and enhancing the outward appearance of the skin of a subject (e.g., a male or female human subject).
A61K 31/192 - Acides carboxyliques, p. ex. acide valproïque ayant des groupes aromatiques, p. ex. sulindac, acides 2-aryl-propioniques, acide éthacrynique
C07C 69/76 - Esters d'acides carboxyliques dont un groupe carboxyle estérifié est lié à un atome de carbone d'un cycle aromatique à six chaînons
A23L 33/105 - Extraits de plantes, leurs doublons artificiels ou leurs dérivés
Provided herein are variant β-1,3-N-acetylglucosaminyltransferase polypeptides capable of producing lacto-n-neotetraose, yeast cells capable of producing one or more human milk oligosaccharides, and methods of making such cells. Also, provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering HMOs generated by the yeast cells.
C12P 19/18 - Préparation de composés contenant des radicaux saccharide préparés par action d'une transférase glycosylique, p. ex. alpha-, bêta- ou gamma-cyclodextrines
C12P 19/04 - Polysaccharides, c.-à-d. composés contenant plus de cinq radicaux saccharide reliés entre eux par des liaisons glucosidiques
C12N 15/67 - Méthodes générales pour favoriser l'expression
C07K 14/24 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant d'Entérobacteriaceae (F), p. ex. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
C10M 107/14 - Polymères d'hydrocarburesPolymères d'hydrocarbures modifiés par oxydation contenant un diène conjugué
C07C 5/03 - Préparation d'hydrocarbures à partir d'hydrocarbures contenant le même nombre d'atomes de carbone par hydrogénation de liaisons doubles carbone-carbone non aromatiques
Provide are modified host cells that are engineered to decrese expression of a product to undetectable levels in the presence of an exogenous agent, and increase expression of the product in the presence of another exogenous agent. The modified yeast strainshost cells do not express detectable levels of a precursor or substrate used to make the product. The product can be a cannabinoid or precursor thereof, and the substrate can be hexanoate. Also provided are methods for making a product using the modified host cells. The modified host cell can be a yeast strain, such as S. cerevisiae.
Provided herein are genetically modified host cells, compositions, and methods for improved production of steviol glycosides. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleic acid expression cassette that expresses an ABC-transporter capable of transporting steviol glycosides to the extracellular space or to the luminal space of an intracellular organelle. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.
C12P 19/56 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un système cyclique condensé d'au moins trois carbocycles, p. ex. daunomycine, adriamycine
Provided herein are fermentation compositions and methods for improved production of vanillin and/or glucovanillin in the presence of para-aminobenzoic acid (PABA, 4-aminobenzoic acid). The compositions and methods described herein provide efficient routes for the production of vanillin and/or glucovanillin and any compound that can be synthesized or biosynthesized from either or both.
Provided herein are fermentation compositions and methods for improved production of vanillin and/or glucovanillin in the presence of para-aminobenzoic acid (PABA, 4-aminobenzoic acid). The compositions and methods described herein provide efficient routes for the production of vanillin and/or glucovanillin and any compound that can be synthesized or biosynthesized from either or both.
The compositions and methods of the disclosure can be used to produce a cannabinoid in a host cell, such as a yeast cell. For example, the disclosure features host cells (e.g., yeast cells) modified to express one or more enzymes of a cannabinoid biosynthetic pathway, such as an acyl activating enzyme (AAE), a tetraketide synthase (TKS), a cannabigerolic acid synthase (CBGaS), and/or an olivetolic acid cyclase (OAC).
The compositions and methods of the disclosure can be used to produce a cannabinoid in a host cell, such as a yeast cell. For example, the disclosure features host cells (e.g., yeast cells) modified to express one or more enzymes of a cannabinoid biosynthetic pathway, such as an acyl activating enzyme (AAE), a tetraketide synthase (TKS), a cannabigerolic acid synthase (CBGaS), and/or an olivetolic acid cyclase (OAC).
Portable fluid dispensing apparatuses are disclosed. In some embodiments, the apparatus includes an upper assembly and a lower assembly. The lower assembly includes a base shell and a lid removably coupled to the base shell. The upper assembly includes an upper shell attached to the lid and having a first aperture. The upper assembly additionally includes a fluid dispenser attached to a neck portion of the lid and received. The fluid dispenser includes an actuator nozzle, a first tube received in a first internal compartment of the base shell, a pump disposed between the nozzle and the first tube. The upper assembly further includes a pump control member having a second tube connected to the nozzle. The pump control member is sized to allow movement of the pump control member and the nozzle toward the pump to activate the pump and dispense fluid stored in the first internal compartment.
A47K 5/12 - Distributeurs de savon pour savon liquide ou pâteux
B05B 11/00 - Appareillages monoblocs tenus à la main dans lesquels l'écoulement du contenu est produit par la force musculaire de l'opérateur au moment de l'utilisation
B05B 11/02 - Membranes ou pistons agissant sur le contenu à l'intérieur du récipient, p. ex. pistons suiveurs
77.
VACCINE ADJUVANTS AND METHODS OF SYNTHESIZING AND USING THE SAME
The disclosure provides compounds useful as adjuvants in vaccines, as well as methods of synthesizing such compounds and methods of using such compounds in the formulation of a vaccine. The disclosure also features methods of administering such vaccines to a subject (e.g., a mammalian subject, such as a human) in order to treat or prevent one or more diseases, such as a disease caused by a viral or bacterial infection.
The disclosure provides compounds useful as adjuvants in vaccines, as well as methods of synthesizing such compounds and methods of using such compounds in the formulation of a vaccine. The disclosure also features methods of administering such vaccines to a subject (e.g., a mammalian subject, such as a human) in order to treat or prevent one or more diseases, such as a disease caused by a viral or bacterial infection.
Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.
Provided herein are sunscreen formulations containing a metal oxide sunscreen, squalane, and an antioxidant. The sunscreen formulations provided have increased SPF per unit mass of the metal oxide compared to sunscreen formulations that contain metal oxide without the addition of squalane and antioxidant. Also provided are methods of preventing UV damages to skin involving the application of the sunscreen formulation.
Provided herein are sunscreen formulations containing a metal oxide sunscreen, squalane, and an antioxidant. The sunscreen formulations provided have increased SPF per unit mass of the metal oxide compared to sunscreen formulations that contain metal oxide without the addition of squalane and antioxidant. Also provided are methods of preventing UV damages to skin involving the application of the sunscreen formulation.
Provided herein are compounds, methods and compositions for determining whether a patient has a neurological disease or disorder is provided, comprising detecting the presence of a detectable target protein, or an accumulated mass thereof, for example, amyloid beta protein or phosphorylated tau protein, or an accumulated mass thereof, in a tissue or a sample of the patient. The detecting may comprise contacting the target protein with a compound described herein.
A61P 25/28 - Médicaments pour le traitement des troubles du système nerveux des troubles dégénératifs du système nerveux central, p. ex. agents nootropes, activateurs de la cognition, médicaments pour traiter la maladie d'Alzheimer ou d'autres formes de démence
The present disclosure relates generally to compositions and methods for determining whether a patient suffers from a traumatic brain injury (TBI) by detecting the presence of an amyloid beta protein in an eye of the patient. Also provided are compositions and methods for preparing a patient for diagnosis and treatment of traumatic brain injury (TB).
A61B 5/145 - Mesure des caractéristiques du sang in vivo, p. ex. de la concentration des gaz dans le sang ou de la valeur du pH du sang
A61B 5/00 - Mesure servant à établir un diagnostic Identification des individus
A61B 3/12 - Appareils pour l'examen optique des yeuxAppareils pour l'examen clinique des yeux du type à mesure objective, c.-à-d. instruments pour l'examen des yeux indépendamment des perceptions ou des réactions du patient pour examiner le fond de l'œil, p. ex. ophtalmoscopes
84.
Stevia rebaudiana kaurenoic acid hydroxylase variants for high efficiency production of rebaudiosides
Stevia rebaudiana kaurenoic acid hydroxylase. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.
C12P 19/56 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un système cyclique condensé d'au moins trois carbocycles, p. ex. daunomycine, adriamycine
Provided herein is the design and synthesis of novel molecular rotor fluorophores useful for detection of amyloid or amyloid like proteins. The fluorophores are designed to exhibit enhanced fluorescence emission upon associating with amyloid or amyloid like proteins as compared to unbound compound. Also disclosed herein are methods for treating diseases associated with amyloid or amyloid like proteins.
C07D 295/155 - Composés hétérocycliques contenant des cycles polyméthylène imine d'au moins cinq chaînons, des cycles aza-3 bicyclo [3.2.2] nonane, piperazine, morpholine ou thiomorpholine, ne comportant que des atomes d'hydrogène liés directement aux atomes de carbone du cycle avec des radicaux hydrocarbonés substitués liés aux atomes d'azote du cycle substitués par des atomes de carbone comportant trois liaisons à des hétéro-atomes avec au plus une liaison à un halogène, p. ex. radicaux ester ou nitrile avec les atomes d'azote du cycle et les atomes de carbone comportant trois liaisons à des hétéro-atomes séparés par des carbocycles ou par des chaînes carbonées interrompues par des carbocycles
C07D 211/06 - Composés hétérocycliques contenant des cycles pyridiques hydrogénés, non condensés avec d'autres cycles avec uniquement des atomes d'hydrogène et de carbone liés directement à l'atome d'azote du cycle ne comportant pas de liaison double entre chaînons cycliques ou entre chaînons cycliques et chaînons non cycliques
C07D 241/04 - Composés hétérocycliques contenant des cycles diazine-1,4 ou diazine-1,4 hydrogéné non condensés avec d'autres cycles ne comportant pas de liaisons doubles entre chaînons cycliques ou entre chaînons cycliques et chaînons non cycliques
C07D 265/30 - Oxazines-1, 4Oxazines-1, 4 hydrogénées non condensées avec d'autres cycles
C07D 295/04 - Composés hétérocycliques contenant des cycles polyméthylène imine d'au moins cinq chaînons, des cycles aza-3 bicyclo [3.2.2] nonane, piperazine, morpholine ou thiomorpholine, ne comportant que des atomes d'hydrogène liés directement aux atomes de carbone du cycle avec des radicaux hydrocarbonés substitués liés aux atomes d'azote du cycle
C07D 307/52 - Radicaux substitués par des atomes d'azote ne faisant par partie d'un radical nitro
C07D 333/22 - Radicaux substitués par des hétéro-atomes liés par une liaison double ou par deux hétéro-atomes, autres que des halogènes, liés au même atome de carbone par des liaisons simples
C07D 401/04 - Composés hétérocycliques contenant plusieurs hétérocycles comportant des atomes d'azote comme uniques hétéro-atomes du cycle, au moins un cycle étant un cycle à six chaînons avec un unique atome d'azote contenant deux hétérocycles liés par une liaison directe de chaînon cyclique à chaînon cyclique
C07D 401/14 - Composés hétérocycliques contenant plusieurs hétérocycles comportant des atomes d'azote comme uniques hétéro-atomes du cycle, au moins un cycle étant un cycle à six chaînons avec un unique atome d'azote contenant au moins trois hétérocycles
C07D 405/04 - Composés hétérocycliques contenant à la fois un ou plusieurs hétérocycles comportant des atomes d'oxygène comme uniques hétéro-atomes du cycle et un ou plusieurs hétérocycles comportant des atomes d'azote comme uniques hétéro-atomes du cycle contenant deux hétérocycles liés par une liaison directe de chaînon cyclique à chaînon cyclique
C07D 405/10 - Composés hétérocycliques contenant à la fois un ou plusieurs hétérocycles comportant des atomes d'oxygène comme uniques hétéro-atomes du cycle et un ou plusieurs hétérocycles comportant des atomes d'azote comme uniques hétéro-atomes du cycle contenant deux hétérocycles liés par une chaîne carbonée contenant des cycles aromatiques
C07D 407/10 - Composés hétérocycliques contenant plusieurs hétérocycles, au moins un cycle comportant des atomes d'oxygène comme uniques hétéro-atomes du cycle, non prévus par le groupe contenant deux hétérocycles liés par une chaîne carbonée contenant des cycles aromatiques
C07D 409/04 - Composés hétérocycliques contenant plusieurs hétérocycles, au moins un cycle comportant des atomes de soufre comme uniques hétéro-atomes du cycle contenant deux hétérocycles liés par une liaison directe de chaînon cyclique à chaînon cyclique
C07D 409/10 - Composés hétérocycliques contenant plusieurs hétérocycles, au moins un cycle comportant des atomes de soufre comme uniques hétéro-atomes du cycle contenant deux hétérocycles liés par une chaîne carbonée contenant des cycles aromatiques
C07D 409/14 - Composés hétérocycliques contenant plusieurs hétérocycles, au moins un cycle comportant des atomes de soufre comme uniques hétéro-atomes du cycle contenant au moins trois hétérocycles
C07D 413/10 - Composés hétérocycliques contenant plusieurs hétérocycles, au moins un cycle comportant des atomes d'azote et d'oxygène comme uniques hétéro-atomes du cycle contenant deux hétérocycles liés par une chaîne carbonée contenant des cycles aromatiques
C07D 413/12 - Composés hétérocycliques contenant plusieurs hétérocycles, au moins un cycle comportant des atomes d'azote et d'oxygène comme uniques hétéro-atomes du cycle contenant deux hétérocycles liés par une chaîne contenant des hétéro-atomes comme chaînons
C07D 295/13 - Composés hétérocycliques contenant des cycles polyméthylène imine d'au moins cinq chaînons, des cycles aza-3 bicyclo [3.2.2] nonane, piperazine, morpholine ou thiomorpholine, ne comportant que des atomes d'hydrogène liés directement aux atomes de carbone du cycle avec des radicaux hydrocarbonés substitués liés aux atomes d'azote du cycle substitués par des atomes d'azote liés par des liaisons simples ou doubles avec les atomes d'azote du cycle et les atomes d'azote substituants liés à la même chaîne carbonée, qui n'est pas interrompue par des cycles carbocycliques à une chaîne acyclique saturée
A61B 5/00 - Mesure servant à établir un diagnostic Identification des individus
A61P 25/28 - Médicaments pour le traitement des troubles du système nerveux des troubles dégénératifs du système nerveux central, p. ex. agents nootropes, activateurs de la cognition, médicaments pour traiter la maladie d'Alzheimer ou d'autres formes de démence
C07C 255/42 - Nitriles d'acides carboxyliques ayant des groupes cyano liés à des atomes de carbone acycliques ayant des groupes cyano liés à des atomes de carbone acycliques d'un squelette carboné contenant au moins un cycle aromatique à six chaînons le squelette carboné étant substitué de plus par des atomes d'azote liés par des liaisons simples et n'étant pas liés de plus à d'autres hétéro-atomes
C07C 255/58 - Nitriles d'acides carboxyliques ayant des groupes cyano liés à des atomes de carbone de cycles aromatiques à six chaînons d'un squelette carboné contenant des groupes cyano et des atomes d'azote, liés par des liaisons simples et n'étant pas liés de plus à d'autres hétéro-atomes, liés au squelette carboné
C07C 255/59 - Nitriles d'acides carboxyliques ayant des groupes cyano liés à des atomes de carbone de cycles aromatiques à six chaînons d'un squelette carboné contenant des groupes cyano et des atomes d'azote, liés par des liaisons simples et n'étant pas liés de plus à d'autres hétéro-atomes, liés au squelette carboné le squelette carboné étant substitué de plus par des atomes d'oxygène liés par des liaisons simples
C07C 311/35 - Sulfonamides, le squelette carboné de la partie acide étant substitué de plus par des atomes d'azote liés par des liaisons simples, ne faisant pas partie de groupes nitro ou nitroso ayant les atomes de soufre des groupes sulfonamide liés à des atomes de carbone acycliques d'un squelette carboné non saturé contenant des cycles
C07C 255/43 - Nitriles d'acides carboxyliques ayant des groupes cyano liés à des atomes de carbone acycliques ayant des groupes cyano liés à des atomes de carbone acycliques d'un squelette carboné contenant au moins un cycle aromatique à six chaînons le squelette carboné étant substitué de plus par des atomes d'azote liés par des liaisons simples et n'étant pas liés de plus à d'autres hétéro-atomes le squelette carboné étant substitué de plus par des atomes d'oxygène liés par des liaisons simples
C07H 13/04 - Composés contenant des radicaux saccharide estérifiés soit par l'acide carbonique ou ses dérivés, soit par des acides organiques, p. ex. acides phosphoniques par des acides carboxyliques comportant les radicaux carboxyle estérifiants liés à des atomes de carbone acycliques
C07H 13/00 - Composés contenant des radicaux saccharide estérifiés soit par l'acide carbonique ou ses dérivés, soit par des acides organiques, p. ex. acides phosphoniques
86.
Compositions and methods for delivering cannabinoids to skin
Provided herein are compositions for delivering one or more cannabinoids to the skin of a subject and methods of using the compositions to deliver the one or more cannabinoids to the skin of a subject. The compositions contain a carrier that facilitates the delivery and permeation of cannabinoids to and through the skin. The carriers of the compositions contain squalane or mixtures of squalane and hemisqualane. The compositions described herein may serve as the base for the preparation of various cosmetics or various pharmaceuticals. The cosmetics or pharmaceuticals may be used to reduce inflammation, control pain, or prevent aging of the skin of a subject.
Provided herein are high potency sweeteners containing at least 95% rebaudioside M generated from Saccharomyces cerevisiae strains engineered to produce high purity rebaudioside M when fermented with sugar cane syrup. In addition, provided are methods of purifying the high potency sweeteners from cleared fermentation broths. Also provided are sugar substitutes containing the high potency sweeteners and methods of making same.
Provided herein are high potency sweeteners containing at least 95% rebaudioside M generated from Saccharomyces cerevisiae strains engineered to produce high purity rebaudioside M when fermented with sugar cane syrup. In addition, provided are methods of purifying the high potency sweeteners from cleared fermentation broths. Also provided are sugar substitutes containing the high potency sweeteners and methods of making same.
Provided herein are sunscreen formulations containing a metal oxide sunscreen, squalane, and an antioxidant. The sunscreen formulations provided have increased SPF per unit mass of the metal oxide compared to sunscreen formulations that contain metal oxide without the addition of squalane and antioxidant. Also provided are methods of preventing UV damages to skin involving the application of the sunscreen formulation.
Provided herein are sunscreen formulations containing a metal oxide sunscreen, squalane, and an antioxidant. The sunscreen formulations provided have increased SPF per unit mass of the metal oxide compared to sunscreen formulations that contain metal oxide without the addition of squalane and antioxidant. Also provided are methods of preventing UV damages to skin involving the application of the sunscreen formulation.
Provided herein are genetically modified host cells, compositions, and methods for improved production of artemisinic acid. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of amorpha-4,11-diene 12-monooxygenase. Also provided herein are methods for screening for variants of cytochrome p450 enzymes that have increased enzymatic activity relative to a parental control enzyme.
The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.
C07K 14/395 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de champignons provenant de levures provenant de Saccharomyces
C12P 19/62 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un hétérocycle autre que saccharide ou à un système cyclique condensé contenant un hétérocycle autre que saccharide, p. ex. coumermycine, novobiocine l'hétérocycle comportant au moins huit chaînons et uniquement l'oxygène comme hétéro-atome du cycle, p. ex. érythromycine, spiramycine, nystatine
C12P 5/02 - Préparation des hydrocarbures acycliques
C12P 7/64 - GraissesHuilesCires de type esterAcides gras supérieurs, c.-à-d. ayant une chaîne continue d'au moins sept atomes de carbone liée à un groupe carboxyleHuiles ou graisses oxydées
C12P 21/00 - Préparation de peptides ou de protéines
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
C12N 15/65 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression utilisant des marqueurs
C12P 21/02 - Préparation de peptides ou de protéines comportant une séquence connue de plusieurs amino-acides, p. ex. glutathion
93.
AMORPHA-4,11-DIENE 12-MONOOXYGENASE VARIANTS AND USES THEREOF
Provided herein are genetically modified host cells, compositions, and methods for improved production of artemisinic acid. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of amorpha-4,11-diene 12-monooxygenase. Also provided herein are methods for screening for variants of cytochrome p450 enzymes that have increased enzymatic activity relative to a parental control enzyme.
A method for recovering one or more water immiscible compounds comprising acidifying and disrupting the microbial biomass; heating the acidified, disrupted microbial biomass to form a heated, acidified disrupted microbial biomass; and contacting the heated, acidified, disrupted microbial biomass with a disulfonated surfactant in an amount sufficient to release at least 30% of the one or more water immiscible compounds from the microbial biomass.
09 - Appareils et instruments scientifiques et électriques
35 - Publicité; Affaires commerciales
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Downloadable computer software for use in influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events by talent agencies; Downloadable computer application software for use in influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events by talent agencies; downloadable computer software platform for use in influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events by talent agencies; downloadable computer software for creating and organizing marketing materials; downloadable software for creating and organizing marketing materials; downloadable computer software for transmission of digital marketing materials; downloadable software for transmission of digital marketing materials; downloadable computer software for creating searchable databases of information and data; downloadable computer software for managing and analyzing marketing lead generation data; downloadable software for use in influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; downloadable computer databases in the field of influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; downloadable electronic databases in the field of influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; computer software for database management; electronic databases in the field of influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events recorded on computer media; downloadable computer software for application and database integration; downloadable computer programs for accessing, browsing and searching online databases; downloadable computer software for creating searchable databases of information and data; downloadable software for data bank management; downloadable computer software for use in influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; downloadable computer software platform for use in influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; downloadable Software for use in influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; downloadable computer software platforms for use in influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; downloadable computer software platforms for collaborating on shared documents; downloadable application Programming interface (API) software; network servers for cloud-computing applications; downloadable Cloud computing software for use in influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; downloadable computer application software used in cloud computing to influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; downloadable operating software and platforms used for digital telephone signal transmission; downloadable computer software platforms for accessing social networking websites; downloadable and recorded computer software platforms for use in influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; downloadable communication software for provided access to the internet; downloadable telecommunications software for transmitting electronic messages; downloadable communications software for use in accessing data stored in the cloud; downloadable mobile application software for use in influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; downloadable software drivers for electronic devices that allow computer hardware and electronic devices to communicate with each other; downloadable graphical user interface software; download software for managing networked computer systems; downloadable communications software for digital signal processing; downloadable communication, networking and social networking software for use in influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; downloadable community software for use in content management on websites; blank data cartridges; wireless transmitters for telecommunications signals; downloadable software for use in transferring and tracking data over data networks; downloadable file sharing software; downloadable computer software and hardware apparatus for downloading, transmitting, receiving, providing, publishing, extracting, encoding, decoding, reading, storing and organizing audiovisual, videographic and written data; downloadable computer software for gathering, processing, monitoring, analyzing, managing and reporting information; downloadable computer software for gathering, processing, monitoring, analyzing, managing and reporting information concerning usage and performance of software, applications, websites, virtual worlds and audio visual content; downloadable computer software for gathering, processing, analyzing, managing and reporting information concerning online, internet and web site activity; downloadable computer software for designing, developing, modifying and improving computer software, applications, websites and audio visual content; pre-recorded electronic, magnetic and optical data carriers, namely, CDs and DVDs featuring talent agency services; downloadable electronic publications in the nature of magazines in the field of software via online databases, internet, websites and computer networks; electronic notice boards; downloadable mobile applications for talent agency services; downloadable software in the nature of a mobile application for use in influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; downloadable computer platform software for accessing social networking websites; downloadable computer software for collecting, organizing, book marking, transmission, storage and sharing of data and information; downloadable digital audio files featuring talent agency services; blank magnetic data carriers, recordable discs, compact discs, DVDs, and USB flash drives Talent agency services, namely, business management of performing artists; business management of performing artists; Business management of musical performers; employment services in the nature of talent casting of performing artists in the fields of music, video and films; Negotiation of commercial transactions for performing artists; Employment recruiting in the nature of booking jobs for performing artists; Advertising and marketing; business administration relating to marketing; Advertising agencies; Advertising agency services; Advertising analysis; Advertising and advertisement services; Advertising and marketing consultancy; Advertising and marketing services provided by means of indirect methods of marketing communications, namely, social media, blogging, and other forms of passive, sharable or viral communications channels; Advertising and promotion services; Advertising and promotion services and related consulting; Advertising and publicity; Advertising, namely, promotion of products and services of third parties through sponsoring arrangements and license agreements; Advisory services for business management; Advisory services relating to advertising; Analysis relating to marketing; blogger outreach services, namely, promoting goods and services of others via online blogs; Business administration consultancy; business administration for others; Business administration services for processing sales made on the internet; Business administration services for the processing of sales made on a global computer network; Business administration services for the processing of sales made on the Internet; Business advice; Business advice relating to advertising; Business advice relating to marketing; Developing promotional campaigns for business; Development and implementation of marketing strategies for others; Development of advertising concepts; Digital advertising services; Digital marketing; Database management and compilation of information on computerised databases; Business management assistance; business management assistance for promoting the goods and services of others; assistance with business management and planning; business management assistance services; Managing and operating businesses for others in the field of influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; Management of computer databases; business management of performing artists Development of computer software, computer software applications, and computer platform software for use in the field of talent agency operation; Platform as a Service (PaaS) featuring computer software platforms for influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; Platform as a Service (PaaS) featuring software platforms for developing marketing campaigns; Software as a service (SaaS) featuring software for organizing marketing data; Software as a service (SaaS) featuring software for managing and analyzing marketing lead generation data; Database design and development; Development of Software for the analysis of business data; Development of Software for the analysis of marketing data; Development of Software platforms for the analysis of business data; Development of Software platforms for the analysis of marketing data; Development of Cloud computing software for the analysis of business data; Development of Cloud computing software for the analysis of marketing data; Development of Software for the modelling of business data; Development of Software for the modelling of marketing data; Development of computer Platform Software for the modelling of business data; Development of computer Platform Software for the modelling of marketing data; Development of Cloud computing software for the modelling of business data; Development of Cloud computing software for the modelling of marketing data; Maintenance of online databases for others; Reconstitution of databases; computer services, namely, hosting computer databases of others on a computer network; Consulting services in the field of software as a service (SaaS) for lead generation and lead management; Software as a service (SaaS) services featuring software for use in influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; Consulting services in the field of software as a service (SaaS); Software development services; software design and development; Software engineering services; software creation; Software customisation services; Computer software integration; Software design for others; Software as a service services featuring software for influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; Computer software advisory services; Computer software consultancy services; Computer software design and development; Development of computer platforms; Hosting of digital content and websites of others on the Internet; Programming of software for Internet platforms; Programming of software for information platforms on the Internet; designing computing platform software for third parties; Software as a service (SaaS) services featuring computer software platforms for use in influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; Computer programming services; Computer software development consisting of the design, maintenance, programming, engineering, research and computer code writing services; advisory and consultancy services relating to computer software and computer software development, design, maintenance, consultancy, programming, engineering, research and computer code writing; Technical data analysis services in the field of software development and design; design and development of computer hardware and software; electronic data storage; Computer services, namely, cloud hosting provider services; application service provider services featuring software application for use in influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; providing temporary use of online non-downloadable software for use in influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; computer consultation; consulting in the field of information technology; computer consultation in the field of cloud computing; hosting online web facilities for others for influencer marketing, influencer strategy, content production, campaign amplification, social media campaigns and branded events; hosting websites
96.
Compositions and methods for delivering cannabinoids to skin
Provided herein are compositions for delivering one or more cannabinoids to the skin of a subject and methods of using the compositions to deliver the one or more cannabinoids to the skin of a subject. The compositions contain a carrier that facilitates the delivery and permeation of cannabinoids to and through the skin. The carriers of the compositions contain squalane or mixtures of squalane and hemisqualane. The compositions described herein may serve as the base for the preparation of various cosmetics or various pharmaceuticals. The cosmetics or pharmaceuticals may be used to reduce inflammation, control pain, or prevent aging of the skin of a subject.
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Providing synthetic biology laboratory services, namely, performing genomic modifications and installing and optimizing biosynthetic pathways in host cells to create, validate, scale-up and commercialize biotechnology products using tools for strain engineering, high throughput screening, analytics, bioinformatics, and process development
98.
COMPOSITIONS AND METHODS FOR DELIVERING CANNABINOIDS TO SKIN
Provided herein are compositions for delivering one or more cannabinoids to the skin of a subject and methods of using the compositions to deliver the one or more cannabinoids to the skin of a subject. The compositions contain a carrier that facilitates the delivery and permeation of cannabinoids to and through the skin. The carriers of the compositions contain squalane or mixtures of squalane and hemisqualane. The compositions described herein may serve as the base for the preparation of various cosmetics or various pharmaceuticals. The cosmetics or pharmaceuticals may be used to reduce inflammation, control pain, or prevent aging of the skin of a subject.
Provided herein antiperspirant composition formulations that contain hemisqualane and lack organosilicon compounds. The hemisqualane provides the carrier and emollient activities that are typically supplied by an organosilicon compound. The antiperspirant formulations provided herein have a benefit of not releasing organosilicon compounds into the environment during and after their use.
Provided herein are compositions for delivering one or more cannabinoids to the skin of a subject and methods of using the compositions to deliver the one or more cannabinoids to the skin of a subject. The compositions contain a carrier that facilitates the delivery and permeation of cannabinoids to and through the skin. The carriers of the compositions contain squalane or mixtures of squalane and hemisqualane. The compositions described herein may serve as the base for the preparation of various cosmetics or various pharmaceuticals. The cosmetics or pharmaceuticals may be used to reduce inflammation, control pain, or prevent aging of the skin of a subject.
