Method for targeted alteration of a duplex acceptor DNA sequence in a plant cell protoplast, comprising combining the duplex acceptor DNA sequence with a donor mutagenic nucleobase, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence and wherein the donor mutagenic nucleobase comprises at least one mismatch with respect to the duplex acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence, wherein the method further comprises a step of introducing the donor mutagenic nucleobase into the cell protoplasts using polyethylene glycol (PEG) mediated transformation and the use of PEG protoplast transformation for enhancing the rate of targeted mutagenesis.
The invention pertains to a method for increasing the accuracy of sequencing, in particular ONT sequencing. The invention relates to a method for determining a sequence of interest in a double- stranded nucleic acid molecule, wherein the method comprises the steps of providing a sample comprising the double-stranded nucleic acid molecule, covalently closing at least one end of the double-stranded nucleic acid molecule to provide a double-stranded nucleic acid molecule having one open end and one closed end, sequencing both strands of at least part of the double-stranded nucleic acid molecule in a single sequencing reaction to generate a duplex read; and generating a consensus sequence from the duplex read to determine the sequence in the double-stranded nucleic acid molecule. Preferably, the sequencing is nanopore sequencing.
The invention pertains to a method for producing a shoot of a plant, wherein the shoot comprises one or more cells having a genetic modification. The method preferably comprises the steps of: i) providing a plant, ii) introducing in a cell of the plant a vector expressing at least one of a gRNA and a CRISPR-nuclease, iii) removing the shoot apical meristem of the plant, and iv) regenerating a shoot at a second location in the plant, wherein the shoot comprises one or more cells having a genetic modification. The shoot apical meristem of the plant is preferably removed by decapitating the plant. Similarly, the shoot is preferably regenerated by a second decapitation of the plant at a second location. The invention further related to a plant having a shoot comprising one or more cells having a genetic modification, wherein the plant is obtainable by the method of the invention.
The invention pertains to a method for increasing plant pollen viability using a MRN-ATM pathway inhibitor. The increased pollen viability preferably results in increased plant viability. The invention further pertains to an MRN-ATM pathway inhibitor for increasing plant pollen viability. A preferred MRN-ATM pathway inhibitor for use in the invention is 2-Amino-5-[(4-hydroxyphenyl)methylene]-4(5H)-thiazolone. The invention also pertains to a method for developing a mature fertile plant graft comprising contacting an isolated plant part comprising an immature flower bud with a (hazardous or toxic) compound.
The present invention provides for a method for producing an inbred plant comprising a first and second trait of interest in the L1-shoot meristem layer for use in producing a periclinal chimera plant, the inbred plant thus obtained, the use of said inbred plant for producing said periclinal chimera plant, a method for producing a periclinal chimera plant using said inbred plant, a periclinal chimera plant thus obtained, the use of said periclinal chimera plant in producing plant product and the plant product thus obtained.
The invention pertains to a mutant NAL1 protein for increasing test weight, and a nucleic acid molecule, chimeric gene, vector and host cell comprising a sequence encoding said mutant NAL1 protein. The invention further pertains to a host cell comprising said mutant protein, nucleic acid molecule, chimeric gene and/or vector, a method for producing a plant having an increased test weight, a method of screening plants having an increased test weight and a plant comprising a sequence encoding the mutant NAL1 protein.
The invention concerns a method for producing a shoot of a plant comprising germline progenitor cells of a recalcitrant plant. The germline progenitor cells may be modified to comprise a mutation in a sequence of interest. The invention further pertains to plants obtainable by the method of the invention, wherein the plant preferably comprises at least the L2-meristem layer of the recalcitrant plant.
The present invention relates to the field of genetics, more in particular to quantitative sequencing data. A method of processing and/or compressing quantitative sequencing data is provided, a computer-readable storage medium to comprise such processed data and a computing device comprising at least one processor configured to process such data.
The invention pertains to a method for increasing the accuracy of sequencing, in particular ONT sequencing. In an aspect, the invention relates to a method for determining a sequence of interest in a double-stranded nucleic acid molecule, wherein the method comprises the steps of providing a sample comprising the double-stranded nucleic acid molecule, covalently closing at least one end of the double-stranded nucleic acid molecule to provide a double-stranded nucleic acid molecule having one open end and one closed end, sequencing both strands of at least part of the double- stranded nucleic acid molecule in a single sequencing reaction to generate a duplex read; and generating a consensus sequence from the duplex read to determine the sequence in the double- stranded nucleic acid molecule. Preferably, the sequencing is nanopore sequencing.
The invention concerns a method for producing a shoot of a plant comprising germline progenitor cells of a recalcitrant plant. The germline progenitor cells may be modified to comprise a mutation in a sequence of interest. The invention further pertains to plants obtainable by the method of the invention, wherein the plant preferably comprises at least the L2-meristem layer of the recalcitrant plant.
The invention concerns the targeted genomic modification of a plant cell, preferably a meristem cell. More in particular, the invention pertains to a vector expressing a coding RNA, wherein the coding RNA comprises a sequence encoding a CRISPR-nuclease and a mobile element, wherein the mobile element enables intercellular translocation of the coding RNA, preferably intercellular translocation to a meristem cell. The invention further concerns an editing RNA comprising the coding RNA and further comprising a guide RNA.
Stevia varieties with a high content of RebM, are disclosed Further provided are methods for producing Stevia plants having a high RebM content by negatively regulating certain genes selecting the resulting plants, and breeding with such plants to confer such desirable Reb M phenotypes to plant progeny.
The invention pertains to a method for producing a plant cell comprising a modified RNA molecule, comprising the steps of i) providing the plant cell; and ii) introducing the modified RNA molecule into the plant cell. The modified RNA molecule comprises a modified uridine, wherein the modified uridine is least one of a pseudo-uridine and a N1-methyl-pseudo-uridine. The modified RNA molecule further preferably comprises a 5'-UTR, a coding sequence, and a 3'-UTR. The 5'-UTR comprises a 5'-UTR of a positive-strand RNA virus or the 5'-UTR of a plant RNA transcript. The 3'-UTR comprises a poly(A) tail. The modified RNA molecule of the invention has an increased expression from the coding sequence as compared to an identical unmodified RNA molecule. The invention further pertains to the modified RNA molecule and a plant cell comprising said modified RNA molecule.
The invention pertains to a method for producing a plant shoot, wherein said method comprises the step of locally contacting a plant tissue comprising a sequence encoding at least one regeneration factor under the control of an inducible promoter with a inductive hydrogel. The invention further pertains to an inductive hydrogel and the use of said inductive hydrogel for inducing regeneration.
OrobancheOrobanche resistance as compared to a control plant. The method comprises the steps of impairing expression and/or activity of a protein selected from the group consisting of a UDP-glucuronate 4 epimerase protein, a protein kinase RLK-Pelle-CrRLK1 L-1 protein, an acid phosphatase protein, an indole-3- pyruvatemonooxygenase protein, an inorganic phosphate transporter protein, a cis- epoxycarotenoid oxygenase protein, and an alcohol acetyltransferase protein. The invention further concerns a plant having an impaired expression and/or activity of at least one of these proteins and a nucleic acid comprising a gene encoding at least one of the proteins, and wherein the gene comprises one or more modifications resulting in impaired expression and/or activity.
The current invention pertains to a method for sequencing of a target nucleic acid fragment from a nucleic acid sample, comprising the steps of cleaving the nucleic acid sample with a first and a second RNA guided or DNA guided endonuclease complex, preferably a first and a second gRNA-CAS complex, thereby generating the target nucleic acid fragment and at least one non-target nucleic acid fragment. The generated fragments are subsequently contacted with an exonuclease, wherein the exonuclease digests only the non-target nucleic acid fragments. Subsequently said target nucleic acid fragment is sequenced using nanopore selective sequencing. The invention further pertains to the use of the enriched target nucleic acid fragments for nanopore selective sequencing the target nucleic acid fragment.
The invention pertains to a method for labelling a target nucleic acid fragment using a combination of a site-specific nuclease and a reverse transcriptase. The labelling results in the addition of a specific nucleotide sequence to at least one free 3′-end of the target nucleic acid fragment. The invention further relates to a method for determining the sequence of the target nucleic acid fragment as well as construct and kit for use in the method of the invention.
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/48 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une transférase
The invention provides a method to produce a mutant gene, wherein said gen comprises a modified promoter and wherein said gene is capable of inducing the parthenogenesis phenotype to a plant. The invention further provides said mutant gene, isolated nucleic acid molecule, construct or vector comprising the same. Also, the invention provides for a method to produce a parthenogenetic plant comprising the mutant gene, and the parthenogenetic plant thus obtained.
Cucumis melo, or powdery mildew-conferring part or variant thereof. The invention also relates to markers for identification of said QTLs, use thereof and methods for producing plants with increased resistance to powdery mildew and the plants thus obtained.
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
C12Q 1/6895 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les plantes, les champignons ou les algues
Fusarium oxysporum f. sp. Cubense Race Fusarium oxysporum f. sp. Cubense Race 1. The invention further pertains to a method for increasing resistance to Panama disease in a banana, a method for producing a banana having increased resistance as well as a banana obtained by such method.
The invention pertains to a method for producing a shoot of a plant, wherein the shoot comprises one or more cells having a genetic modification. The method preferably comprises the steps of: i) providing a plant, ii) introducing in a cell of the plant a vector expressing at least one of a gRNA and a CRISPR-nuclease, iii) removing the shoot apical meristem of the plant, and iv) regenerating a shoot at a second location in the plant, wherein the shoot comprises one or more cells having a genetic modification. The shoot apical meristem of the plant is preferably removed by decapitating the plant. Similarly, the shoot is preferably regenerated by a second decapitation of the plant at a second location. The invention further related to a plant having a shoot comprising one or more cells having a genetic modification, wherein the plant is obtainable by the method of the invention.
The invention pertains to a method for increasing plant pollen viability using a MRN-ATM pathway inhibitor. The increased pollen viability preferably results in increased plant viability. The invention further pertains to an MRN-ATM pathway inhibitor for increasing plant pollen viability. A preferred MRN-ATM pathway inhibitor for use in the invention is 2-Amino-5-[(4-hydroxyphenyl)methylene]- 4(5H)-thiazolone. The invention also pertains to a method for developing a mature fertile plant graft comprising contacting an isolated plant part comprising an immature flower bud with a (hazardous or toxic) compound.
The invention pertains to a method for increasing plant pollen viability using a MRN-ATM pathway inhibitor. The increased pollen viability preferably results in increased plant viability. The invention further pertains to an MRN-ATM pathway inhibitor for increasing plant pollen viability. A preferred MRN-ATM pathway inhibitor for use in the invention is 2-Amino-5-[(4-hydroxyphenyl)methylene]- 4(5H)-thiazolone. The invention also pertains to a method for developing a mature fertile plant graft comprising contacting an isolated plant part comprising an immature flower bud with a (hazardous or toxic) compound.
A plant selection apparatus has a storage means for storing a plurality of image datasets of plants and associated plant information including at least one of genotype information of the plants, phenotype information of the plants, and pedigree information of the plants. A selection unit preselects a subset of the plants. A display device displays the image datasets of the subset of the plants. A tracking unit generates observation information including information on which of the subset of the plants is observed, and the storage means is configured for storing the observation information. A training unit trains a classifier based on the observation information, the input selection, and the plant information including said at least one of the genotype information, the phenotype information, and the pedigree information.
G06K 9/62 - Méthodes ou dispositions pour la reconnaissance utilisant des moyens électroniques
G06V 10/22 - Prétraitement de l’image par la sélection d’une région spécifique contenant ou référençant une formeLocalisation ou traitement de régions spécifiques visant à guider la détection ou la reconnaissance
The invention relates to a method for preparing a sample (400) comprising sample polynucleotides (602) for sequencing, wherein at least one of the sample polynucleotides comprises a known sequence (605), the method comprising: protecting (102) the ends of the sample polynucleotides, contacting (104) at least first and second nucleoprotein particles (608, 702, 704) with the protected sample polynucleotides, wherein each first nucleoprotein particle comprises an effector protein and a first guide polynucleotide (706) and each second nucleoprotein particle comprises an effector protein and a second guide polynucleotide (708), wherein the sequences of the first and second guide polynucleotides are different and are selected such that o the first guide polynucleotides cause the effector proteins to cut the known sequence at a first cleavage site (722); o the second guide polynucleotides cause the effector proteins to cut the known sequence at a second cleavage site (724); o wherein the first and second cleavage sites define an in-between sequence (614) as the part of the known sequence between the first and second cleavage sites (722, 724); o whereby the first and second guide polynucleotides respectively comprise a binding sequence (715, 713) whose position and orientation within the known sequence is selected such that when the sample is contacted with a sequencing adapter (616), the sequencing adapter selectively binds to the ends of the sample polynucleotide fragments created by the cutting which do not comprise the in- between sequence; adding (106) sequencing adapters to the sample.
The current invention pertains to adapters comprising a protelomerase recognition sequence, preferably a TeIN protelomerase recognition sequence. The adapters of the invention can be used for the preparation of a nucleic acid molecule library. The invention also relates to a method for producing a nucleic acid molecule library using one or more adapters comprising a protelomerase recognition sequence. The adapters may be contacted with a protelomerase to cleave and close the ends of the adapters. Said closed adapters are e.g. protected against exonuclease treatment. The method of the invention further concerns an amplification method and a sequencing method using adapters having a protelomerase recognition sequence.
The invention concerns the targeted genomic modification of a plant cell, preferably a meristem cell. More in particular, the invention pertains to a vector expressing a coding RNA, wherein the coding RNA comprises a sequence encoding a CRISPR-nuclease and a mobile element, wherein the mobile element enables intercellular translocation of the coding RNA, preferably intercellular translocation to a meristem cell. The invention further concerns an editing RNA comprising the coding RNA and further comprising a guide RNA.
The invention concerns a method for producing a shoot of a plant comprising germline progenitor cells of a recalcitrant plant. The germline progenitor cells may be modified to comprise a mutation in a sequence of interest. The invention further pertains to plants obtainable by the method of the invention, wherein the plant preferably comprises at least the L2-meristem layer of the recalcitrant plant.
The invention concerns a method for producing a shoot of a plant comprising germline progenitor cells of a recalcitrant plant. The germline progenitor cells may be modified to comprise a mutation in a sequence of interest. The invention further pertains to plants obtainable by the method of the invention, wherein the plant preferably comprises at least the L2-meristem layer of the recalcitrant plant.
The invention is in the field of agriculture, in particular in the field of crop improvement for processing, more particularly in the field of sesquiterpene lactone (STL), squalene and phenolic compound biosynthesis by plants. A method for producing a plant having reduced STL levels, increased squalene levels and increased phenolic compound levels is disclosed, as well as a plant produced by such method.
The invention pertains to a method for isolating a nucleic acid, wherein the nucleic acid is stabilized in a hydrogel. The hydrogel can be dissolved to release the nucleic acid without breaking the molecule. A preferred hydrogel is alginate. The invention further concerns a method for sequencing the nucleic acid and a composition comprising the hydrogel and the nucleic acid.
The present invention provides methods for the production of plant inbred seed, or for the production of plant hybrid seed, wherein the seed obtained exhibits altered and/or improved germination properties, in particular improved germination properties such as enhanced seed germination rate, enhanced seed germination capacity and/or enhanced seedling fresh weight. The present invention also provides for the use of periclinal chimera plants for improving germination properties of seed.
Stevia plants having a high RebM content by negatively regulating certain genes selecting the resulting plants, and breeding with such plants to confer such desirable Reb M phenotypes to plant progeny.
The invention provides nucleotide sequences and amino acid sequences of the Dip gene as well as (functional) homologues, fragments and variants thereof, which provides diplospory as a part of apomixis. Also diplospory plants and methods for making these are provided, as are methods of using these, and methods of making apomictic seed.
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6895 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les plantes, les champignons ou les algues
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
C12N 15/62 - Séquences d'ADN codant pour des protéines de fusion
35.
TARGETED ENRICHMENT USING NANOPORE SELECTIVE SEQUENCING
The current invention pertains to a method for sequencing of a target nucleic acid fragment from a nucleic acid sample, comprising the steps of cleaving the nucleic acid sample with a first and a second RNA guided or DNA guided endonuclease complex, preferably a first and a second gRNA-CAS complex, thereby generating the target nucleic acid fragment and at least one non-target nucleic acid fragment. The generated fragments are subsequently contacted with an exonuclease, wherein the exonuclease digests only the non-target nucleic acid fragments. Subsequently said target nucleic acid fragment is sequenced using nanopore selective sequencing. The invention further pertains to the use of the enriched target nucleic acid fragments for nanopore selective sequencing the target nucleic acid fragment.
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
Computer software; computer software for the acquisition,
analysis and processing of scientific data, biological data
and biotechnological data; computer software for use in
nucleic acid and amino acid analysis; computer software
relating to information concerning nucleic acid sequences of
plants, animals, and microorganisms; computer software for
the acquisition, analysis and processing of data in the
fields of plant and animal reproduction, the agri-foods
industry and research; computer software for the analysis of
nucleic acids and amino acids, the aforesaid software being
used in the fields of plant and animal reproduction, the
agri-foods industry and research. Scientific services; scientific research services; design
services; engineering services; laboratory services;
chemical analysis; scientific analysis; technical data
analysis; biological analysis; design and development of
computer software; maintenance of computer software;
computer software rental; design and development of computer
databases, all aforementioned services in the fields of
biology, biotechnology, chemistry, and agri-foods sectors. Agricultural services; horticultural services; forestry
services; in vitro reproduction of plant cells.
The invention provides a method to produce a mutant gene, wherein said gene comprises a modified promoter and wherein said gene is capable of inducing the parthenogenesis phenotype to a plant. The invention further provides said mutant gene, isolated nucleic acid molecule, construct or vector comprising the same. Also, the invention provides for a method to produce a parthenogenetic plant comprising the mutant gene, and the parthenogenetic plant thus obtained. Mutant genes are based on the PAR gene of Taraxacum officinale having Seq. ID No. 5 and orthologues thereof..
The invention provides a method to produce a mutant gene, wherein said gene comprises a modified promoter and wherein said gene is capable of inducing the parthenogenesis phenotype to a plant. The invention further provides said mutant gene, isolated nucleic acid molecule, construct or vector comprising the same. Also, the invention provides for a method to produce a parthenogenetic plant comprising the mutant gene, and the parthenogenetic plant thus obtained. Mutant genes are based on the PAR gene of Taraxacum officinale having Seq. ID No. 5 and orthologues thereof..
The invention pertains to a method for labelling a target nucleic acid fragment using a combination of a site-specific nuclease and a reverse transcriptase. The labelling results in the addition of a specific nucleotide sequence to at least one free 3'-end of the target nucleic acid fragment. The invention further relates to a method for determining the sequence of the target nucleic acid fragment as well as construct and kit for use in the method of the invention.
The invention provides the nucleotide sequence and amino acid sequences of the parthenogenesis gene of Taraxacum as well as (functional) homologues, fragments and variants thereof, which provides parthenogenesis as a part of apomixis. Also parthenogenetic plants and methods for making these are provided, as are molecular markers and methods of using these.
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
downloadable or recorded computer software for the acquisition, analysis and processing of scientific data, biological data and biotechnological data; downloadable or recorded computer software for use in nucleic acid and amino acid analysis; downloadable or recorded computer software for providing information concerning nucleic acid sequences of plants, animals, and microorganisms; downloadable or recorded computer software for the acquisition, analysis and processing of data in the fields of plant and animal reproduction, the agri-foods industry and research; downloadable or recorded computer software for the analysis of nucleic acids and amino acids, the aforesaid software being used in the fields of plant and animal reproduction, the agri-foods industry and research Scientific services, namely scientific research for innovation for crop improvement; Research services, namely technology research for innovation for crop improvement; Design services, namely scientific design services in the field of crop improvement innovation; Design services namely scientific, technological and engineering design relating to the creation of elementary cells and tissue; Engineering design and drawing services; scientific laboratory services; Chemical analysis; Scientific analysis, namely Scientific analysis of scientific data, biological data and biotechnological data for innovation for crop improvement; Technical data analysis of scientific laboratory data, biological data and biotechnological data; Biological analysis; Design and development of computer software; Maintenance of computer software; Computer software rental for computer software for the acquisition, analysis and processing of scientific data, biological data and biotechnological data, computer software for use in nucleic acid and amino acid analysis, computer software for providing information concerning nucleic acid sequences of plants, animals, and microorganisms, computer software for the acquisition, analysis and processing of data in the fields of plant and animal reproduction, the agri-foods industry and research, software for the analysis of nucleic acids and amino acids, the aforesaid software being used in the fields of plant and animal reproduction, the agri-foods industry and research; Design and development of computer databases; all aforementioned services in the fields of biology, biotechnology, chemistry, and agri-foods sectors Agricultural services, namely agricultural advice on innovation for crop improvement; horticultural services; forestry services in the nature of pest control; in vitro reproduction of plant cells
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
(1) Downloadable and recorded computer software for the acquisition, analysis and processing of scientific data, biological data and biotechnological data; downloadable and recorded computer software for use in nucleic acid and amino acid analysis; downloadable and recorded computer software for providing information concerning nucleic acid sequences of plants, animals, and microorganisms; downloadable and recorded computer software for the acquisition, analysis and processing of data in the fields of plant and animal reproduction, the agri-foods industry and research; downloadable and recorded computer software for the analysis of nucleic acids and amino acids, the aforesaid software being used in the fields of plant and animal reproduction, the agri-foods industry and research. (1) Scientific services, namely scientific research for innovation for crop improvement; research services, namely technology research for innovation for crop improvement; design services, namely scientific design services in the field of crop improvement innovation; design services namely scientific, technological and engineering design relating to the creation of elementary cells and tissue, for crop research and development; engineering design and drawing services namely scientific, technological and engineering design relating to the creation of elementary cells and tissue, for crop research and development; scientific laboratory research and development services; chemical analysis; scientific analysis, namely scientific analysis of scientific data, biological data and biotechnological data for innovation for crop improvement; technical data analysis of scientific laboratory data, biological data and biotechnological data; biological analysis of genetic material, cells and tissues for research and development purposes; design and development of computer software; maintenance of computer software; computer software rental for computer software for the acquisition, analysis and processing of scientific data, biological data and biotechnological data, computer software for use in nucleic acid and amino acid analysis, computer software for providing information concerning nucleic acid sequences of plants, animals, and microorganisms, computer software for the acquisition, analysis and processing of data in the fields of plant and animal reproduction, the agri-foods industry and research, software for the analysis of nucleic acids and amino acids, the aforesaid software being used in the fields of plant and animal reproduction, the agri-foods industry and research; design and development of computer databases; all aforementioned services in the fields of biology, biotechnology, chemistry, and agri-foods sectors.
(2) Agricultural services, namely agricultural advice on innovation for crop improvement; horticultural services; forestry services in the nature of pest control; in vitro reproduction of plant cells.
The current invention pertains to a method for the enrichment of a target nucleic acid fragment from a nucleic acid sample, comprising the steps of cleaving the nucleic acid sample with a first and a second RNA guided or DNA guided endonuclease complex, preferably a first and a second gRNA-CAS complex, thereby generating the target nucleic acid fragment and at least one non-target nucleic acid fragment. The generated fragments are subsequently contacted with an exonuclease, wherein the exonuclease digests only the non-target nucleic acid fragments. The invention further pertains to the use of the enriched target nucleic acid fragments for preparing an adapter ligated target nucleic acid fragment and for sequencing the target nucleic acid fragment.
The current invention pertains to a reliable method for determining the relative frequency of a sequence variant of interest in a nucleic acid sample derived from at least one polyploid cell, wherein the method uses a UMI to correct for any amplification biases. The invention further pertains to the use of a UMI for accurately determining the relative frequency of a sequence variant of interest in a nucleic acid sample derived from at least one polyploid cell.
The invention pertains to a method for targeted modification of DNA in a plant cell, comprising a step of contacting the DNA with an RNA-guided CRISPR-system nuclease complex, wherein the complex comprises a crRNA and a tracrRNA as separate molecules. The invention further pertains to said RNA-guided CRISPR-system nuclease complex for targeting of DNA in a plant cell and kits comprising the RNA-guided CRISPR-system nuclease complex or constructs encoding the same.
Method for targeted alteration of a duplex acceptor DNA sequence in a plant cell protoplast, comprising combining the duplex acceptor DNA sequence with a donor mutagenic nucleobase, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence and wherein the donor mutagenic nucleobase comprises at least one mismatch with respect to the duplex acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence, wherein the method further comprises a step of introducing the donor mutagenic nucleobase into the cell protoplasts using polyethylene glycol (PEG) mediated transformation and the use of PEG protoplast transformation for enhancing the rate of targeted mutagenesis.
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
44 - Services médicaux, services vétérinaires, soins d'hygiène et de beauté; services d'agriculture, d'horticulture et de sylviculture.
Produits et services
computer software; Computer software for the acquisition, analysis and processing of scientific data, biological data and biotechnological data; Computer software for use in nucleic acid and amino acid analysis; Computer software relating to information concerning nucleic acid sequences of plants, animals, and microorganisms; computer software for the acquisition, analysis and processing of data in the fields of plant and animal reproduction, the agri-foods industry and research; computer software for the analysis of nucleic acids and amino acids, the aforesaid software being used in the fields of plant and animal reproduction, the agri-foods industry and research. Scientific services; Research services; Design services; Engineering services; Laboratory services; Chemical analysis; Scientific analysis; Technical data analysis; Biological analysis; Design and development of computer software; Maintenance of computer software; Computer software rental; Design and development of computer databases, all aforementioned services in the fields of biology, biotechnology, chemistry, and agri-foods sectors. agricultural services; horticultural services; forestry services; in vitro reproduction of plant cells.
48.
TYPE V CRISPR/NUCLEASE-SYSTEM FOR GENOME EDITING IN PLANT CELLS
The invention relates to a kit of parts for detecting one or more polymorphisms in a plurality of target nucleotide sequences in a plurality of samples, wherein the kit of parts comprises (i) a first probe and a second probe, wherein the first probe comprises a first target specific section and a first tag section that is non-complementary to the target nucleotide sequence and that comprises a first universal primer binding site, wherein the second probe comprises a second target specific section and a second tag section that is non-complementary to the target nucleotide sequence; and (ii) a first primer and optionally a second primer wherein the first primer comprises a sample-specific identifier sequence and wherein the first primer can hybridize to the first universal primer binding site.
C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p. ex. séquençage par hybridation [SBH]
C12Q 1/683 - Tests d’hybridation pour la détection de mutation ou de polymorphisme faisant intervenir des enzymes de restriction, p. ex. polymorphisme de longueur de fragment de resctriction
50.
STRATEGIES FOR HIGH THROUGHPUT IDENTIFICATION AND DETECTION OF POLYMORPHISMS
The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
C40B 30/04 - Procédés de criblage des bibliothèques en mesurant l'aptitude spécifique à se lier à une molécule cible, p. ex. liaison anticorps-antigène, liaison récepteur-ligand
C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p. ex. séquençage par hybridation [SBH]
C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
The current invention pertains to adapters comprising a protelomerase recognition sequence, preferably a TeIN protelomerase recognition sequence. The adapters of the invention can be used for the preparation of a nucleic acid molecule library. The invention also relates to a method for producing a nucleic acid molecule library using one or more adapters comprising a protelomerase recognition sequence. The adapters may be contacted with a protelomerase to cleave and close the ends of the adapters. Said closed adapters are e.g. protected against exonuclease treatment. The method of the invention further concerns an amplification method and a sequencing method using adapters having a protelomerase recognition sequence.
The current invention pertains to adapters comprising a protelomerase recognition sequence, preferably a TeIN protelomerase recognition sequence. The adapters of the invention can be used for the preparation of a nucleic acid molecule library. The invention also relates to a method for producing a nucleic acid molecule library using one or more adapters comprising a protelomerase recognition sequence. The adapters may be contacted with a protelomerase to cleave and close the ends of the adapters. Said closed adapters are e.g. protected against exonuclease treatment. The method of the invention further concerns an amplification method and a sequencing method using adapters having a protelomerase recognition sequence.
The invention is in the field of agriculture, in particular in the field of crop improvement for processing, more particularly in the field of sesquiterpene lactone (STL), squalene and phenolic compound biosynthesis by plants. A method for producing a plant having reduced STL levels, increased squalene levels and increased phenolic compound levels is disclosed, as well as a plant produced by such method.
The invention pertains to a method for isolating a nucleic acid, wherein the nucleic acid is stabilized in a hydrogel. The hydrogel can be dissolved to release the nucleic acid without breaking the molecule. A preferred hydrogel is alginate. The invention further concerns a method for sequencing the nucleic acid and a composition comprising the hydrogel and the nucleic acid.
The invention concerns a method for the production of oligonucleotides. The method of the invention uses a combination of amplification, restriction and affinity purification to produce high quality oligonucleotides. The invention further pertains to a nucleic acid precursor for use in the method of the invention, a solid support comprising said nucleic acid precursor and a kit for use in the method of the invention.
A plant selection apparatus comprises a storage means (125) for storing a plurality of image datasets (121) of plants (6) and associated plant information (122) including at least one of genotype information of the plants (6), phenotype information of the plants (6), and pedigree information of the plants (6). A selection unit (126) preselects a subset of the plants (6). A display device (14) displays the image datasets of the subset of the plants (6). A tracking unit (127) generates observation information comprising information on which of the subset of the plants is observed, wherein the storage means is configured for storing the observation information. A training unit (128) trains a classifier based on the observation information, the input selection, and the plant information including said at least one of the genotype information, the phenotype information, and the pedigree information.
The current disclosure relates to the field of plants, in particular to the fields of plant breeding and plant genetics. More particular, the disclosure concerns inventive methodology that may be useful in improving plant properties. In particular the invention may be useful in removing linkage drag. Also provided are plant and plant parts obtained with the method disclosed herein.
It was found that plants with loss of functional Msi2 protein due to a nucleotide polymorphism resulting in the introduction of a premature stop codon in the Msi2 protein, are able to induce haploid offspring after a cross to or with a wild type plant comprising a functional Msi2 protein. The invention relates to generation of haploid and doubled haploid plants.
Genetic modification of plants is hampered by the limited capacity of plant cells to regenerate. The current invention solves this problem by introducing or increasing the expression of a histidine kinase in a plant cell. Preferred histidine kinases are at least one of CHK2, CHK3 and CHK4. The invention therefore concerns a method for improving a cytokinin-induced regeneration capacity of a plant cell, wherein the method comprises a step of increasing or introducing the expression of a histidine kinase in the plant cell. The invention further pertains to a method for regenerating a plant, wherein the method comprises a step of introducing or increasing the expression of a histidine kinase and to a plant obtainable from such method. Moreover, the method concerns the use of at least one of CHK2, CHK3 and CHK4 for improving a cytokinin-induced regeneration capacity of a plant.
The invention pertains to a method for regenerating a plant cell, preferably regenerating a shoot from a plant cell by altering the expression levels of at least WOX5 and a PLT protein, preferably WOX5 and PLT1. In addition the expression levels of further proteins can altered, such as WIND1, SHR, SCR, RBR, PLT4 and PLT5 to regenerate a shoot from a plant cell. Preferably, the expression levels are transiently altered. The invention further pertains to a nucleic acid construct suitable for transient protein expression and the use of the protein combinations for regenerating a shoot from a plant cell.
Cucumis melo, or powdery mildew-conferring part or variant thereof. The invention also relates to markers for identification of said QTLs, use thereof and methods for producing plants with increased resistance to powdery mildew and the plants thus obtained.
C12Q 1/6895 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les plantes, les champignons ou les algues
A dicot plant cell, plant tissue or plant having a reduced expression of a wild type protein having at least 75% sequence identity with SEQ ID NO. 1 is disclosed. Also disclosed is a plant cell, plant tissue or plant comprising a modified protein and/or a truncated protein having at least about 75% sequence identity when calculated over the positions corresponding to positions 1-66 and positions 78-308 of SEQ ID NO: 1. The modified protein increases the resistance to Geminiviridae. Nucleic acid constructs encoding the modified or truncated protein are also provided. Lastly, methods for generating a plant cell, plant tissue or plant comprising the modified protein that increases the resistance to Geminiviridae are disclosed.
The invention provides the nucleotide sequence and amino acid sequences of the parthenogenesis gene of Taraxacum as well as (functional) homologues, fragments and variants thereof, which provides parthenogenesis as a part of apomixis. Also parthenogenetic plants and methods for making these are provided, as are molecular markers and methods of using these.
The invention provides the nucleotide sequence and amino acid sequences of the parthenogenesis gene of Taraxacum as well as (functional) homologues, fragments and variants thereof, which provides parthenogenesis as a part of apomixis. Also parthenogenetic plants and methods for making these are provided, as are molecular markers and methods of using these.
Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.
The current invention pertains to a reliable method for determining the relative frequency of a sequence variant of interest in a nucleic acid sample derived from at least one polyploid cell, wherein the method uses a UMI to correct for any amplification biases. The invention further pertains to the use of a UMI for accurately determining the relative frequency of a sequence variant of interest in a nucleic acid sample derived from at least one polyploid cell.
C12Q 1/6895 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les plantes, les champignons ou les algues
G16B 20/20 - Détection d’allèles ou de variantes, p. ex. détection de polymorphisme d’un seul nucléotide
The current invention pertains to a reliable method for determining the relative frequency of a sequence variant of interest in a nucleic acid sample derived from at least one polyploid cell, wherein the method uses a UMI to correct for any amplification biases. The invention further pertains to the use of a UMI for accurately determining the relative frequency of a sequence variant of interest in a nucleic acid sample derived from at least one polyploid cell.
C12Q 1/6895 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les plantes, les champignons ou les algues
G16B 20/20 - Détection d’allèles ou de variantes, p. ex. détection de polymorphisme d’un seul nucléotide
The invention pertains to the targeted alteration of a duplex DNA in a cell, whereby two site-specific nucleases generate an indel, such that the open reading frame is not altered after the second indel. The invention further pertains to the use of such nucleases for the targeted alteration of an open reading frame in duplex DNA and a kit of parts for use in a method of the invention. Using the method of the invention, novel plants were obtained having an improved herbicide resistance. The invention therefore also concerns plants having improved herbicide resistance due to the expression of an altered ALS protein.
09 - Appareils et instruments scientifiques et électriques
35 - Publicité; Affaires commerciales
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Computer software; computer software for creating searchable
databases of information and data; computer software
programs for database management; downloadable mobile
applications for managing data. Compilation and systematisation of information in databases. Design, development and maintenance of databases; providing
online non-downloadable software for database management.
The invention is in the field of agriculture, in particular in the field of crop protection, more particularly in the field of providing nematode resistance to plants. A method for producing a plant having improved nematode resistance, particularly to root-knot nematodes and/or cyst nematodes, is disclosed, as well as a plant produced by such method.
The current invention relates to methods of targeted genetic alteration in cells, preferably plant cells, as well as to plant cells and plants thus obtained using at least a fusion protein comprising a site-specific nuclease domain and a deaminase domain, or a construct encoding the same. The method also provides for a composition and a kit comprising a combination of a first fusion protein comprising a cytosine deaminase domain and a second fusion protein comprising an adenine deaminase domain, preferably for use in the method of the invention. The method provides for targeted alteration of a DNA duplex in plant cells with increased efficacy.
The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
C40B 30/04 - Procédés de criblage des bibliothèques en mesurant l'aptitude spécifique à se lier à une molécule cible, p. ex. liaison anticorps-antigène, liaison récepteur-ligand
C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p. ex. séquençage par hybridation [SBH]
C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
The invention relates to a kit for use in a method for detecting genetic variation in one or more members of a population, comprising (a) an adaptor adapted for ligation to a plurality of nucleic acid fragments and (b) a set of primers for PCR amplification having a 5′-end and a 3′-end, wherein at least one of the primers comprises one or more selective nucleotides at the 3′ end, and wherein the adaptor and/or at least one of the primers comprises a sample-specific identifier sequence capable of indicating sample origin of an amplification product.
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
The current invention pertains to a method for the enrichment of a target nucleic acid fragment from a nucleic acid sample, comprising the steps of cleaving the nucleic acid sample with a first and a second RNA guided or DNA guided endonuclease complex, preferably a first and a second gRNA-CAS complex, thereby generating the target nucleic acid fragment and at least one non-target nucleic acid fragment. The generated fragments are subsequently contacted with an exonuclease, wherein the exonuclease digests only the non-target nucleic acid fragments. The invention further pertains to the use of the enriched target nucleic acid fragments for preparing an adapter ligated target nucleic acid fragment and for sequencing the target nucleic acid fragment.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
The current invention pertains to a method for the enrichment of a target nucleic acid fragment from a nucleic acid sample, comprising the steps of cleaving the nucleic acid sample with a first and a second RNA guided or DNA guided endonuclease complex, preferably a first and a second gRNA-CAS complex, thereby generating the target nucleic acid fragment and at least one non-target nucleic acid fragment. The generated fragments are subsequently contacted with an exonuclease, wherein the exonuclease digests only the non-target nucleic acid fragments. The invention further pertains to the use of the enriched target nucleic acid fragments for preparing an adapter ligated target nucleic acid fragment and for sequencing the target nucleic acid fragment.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
09 - Appareils et instruments scientifiques et électriques
35 - Publicité; Affaires commerciales
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Downloadable computer Software for creating searchable databases of information and data; Downloadable computer Software programs for database management Compilation and systematisation of information in databases Design, development and maintenance of databases; Providing on-line non-downloadable software for database management; Testing of Computer Software; Providing temporary use of non-downloadable computer software for creating searchable databases of information and data; Providing temporary use of non-downloadable computer software programs for database management
09 - Appareils et instruments scientifiques et électriques
35 - Publicité; Affaires commerciales
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Computer Software; Computer Software for creating searchable databases of information and data; Computer Software programs for database management; Downloadable mobile applications for managing data. Compilation and systematisation of information in databases. Design, development and maintenance of databases; Providing on-line non-downloadables software for database management.
78.
DUAL GUIDE RNA FOR CRISPR/CAS GENOME EDITING IN PLANTS CELLS
The invention pertains to a method for targeted modification of DNA in a plant cell, comprising a step of contacting the DNA with an RNA-guided CRISPR-system nuclease complex, wherein the complex comprises a crRNA anda tracrRNA as separate molecules. The invention further pertains to said RNA-guided CRISPR-system nuclease complex for targeting of DNA in a plant cell and kits comprising the RNA-guided CRISPR-system nuclease complex or constructs encoding the same.
Stevia varieties with a high content of RebM, are disclosed Further provided are methods for producing Stevia plants having a high RebM content by negatively regulating certain genes selecting the resulting plants, and breeding with such plants to confer such desirable Reb M phenotypes to plant progeny.
The current invention relates to methods of targeted genetic alteration in plant cells, as well as to plant cells and plants thus obtained. In the method, a Cpf1 protein and a crRNA is employed to provide for targeted alteration, in particular increased biallelic alteration, of a DNA duplex with increased efficacy.
The invention relates to a kit of parts for detecting one or more polymorphisms in a plurality of target nucleotide sequences in a plurality of samples, wherein the kit of parts comprises (i) a first probe and a second probe, wherein the first probe comprises a first target specific section and a first tag section that is non-complementary to the target nucleotide sequence and that comprises a first universal primer binding site, wherein the second probe comprises a second target specific section and a second tag section that is non-complementary to the target nucleotide sequence; and (ii) a first primer and optionally a second primer wherein the first primer comprises a sample-specific identifier sequence and wherein the first primer can hybridize to the first universal primer binding site.
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p. ex. séquençage par hybridation [SBH]
C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
C12Q 1/683 - Tests d’hybridation pour la détection de mutation ou de polymorphisme faisant intervenir des enzymes de restriction, p. ex. polymorphisme de longueur de fragment de resctriction
82.
Methods for the production of seed with improved seed germination properties
The present invention provides methods for the production of plant inbred seed, or for the production of plant hybrid seed, wherein the seed obtained exhibits altered and/or improved germination properties, in particular improved germination properties such as enhanced seed germination rate, enhanced seed germination capacity and/or enhanced seedling fresh weight. The present invention also provides for the use of periclinal chimera plants for improving germination properties of seed.
The present invention provides for a method for producing an inbred plant comprising a first and second trait of interest in the L1-shoot meristem layer for use in producing a periclinal chimera plant, the inbred plant thus obtained, the use of said inbred plant for producing said periclinal chimera plant, a method for producing a periclinal chimera plant using said inbred plant, a periclinal chimera plant thus obtained, the use of said periclinal chimera plant in producing plant product and the plant product thus obtained.
A system for monitoring plants comprises an input unit, an output unit, and a processor that provides (201) image data (107) for a plurality of plants, each image data being associated with an individual plant. It associates (204) the image data (107) of each plant with corresponding plant characteristic data (109). It selects (206) a subset of the plants based on the plant characteristic data (109). It generates (207) a plurality of computer graphics objects corresponding to the selected plants. It applies (208) the image data (107) of each selected plant in form of a computer graphics texture to the corresponding computer graphics object. It determines (209) a position of each computer graphics object in a three-dimensional space of a computer graphics scene. It creates (210) a computer graphics rendering of the scene. It displays (211) the computer graphics rendering.
The invention concerns a method for the production of oligonucleotides. The method of the invention uses a combination of amplification, restriction and affinity purification to produce high quality oligonucleotides. The invention further pertains to a nucleic acid precursor for use in the method of the invention, a solid support comprising said nucleic acid precursor and a kit for use in the method of the invention.
The invention concerns a method for the production of oligonucleotides. The method of the invention uses a combination of amplification, restriction and affinity purification to produce high quality oligonucleotides. The invention further pertains to a nucleic acid precursor for use in the method of the invention, a solid support comprising said nucleic acid precursor and a kit for use in the method of the invention.
Disclosed is a new method of providing plant cells with a targeted alteration in a DNA molecule. The method comprises contacting a population of plant cells comprising a DNA molecule, the DNA molecule having a target sequence, with an aqueous medium, wherein the aqueous medium comprises a CRISPR associated protein (CAS protein) or a CAS-like protein, and a CRISPR-Cas system guide RNA that hybridizes with the target sequence, and wherein the aqueous medium comprises polyethylene glycol (PEG), but needs to be substantially free of glycerol.
The invention pertains to a method for regenerating a plant cell, preferably regenerating a shoot from a plant cell by altering the expression levels of at least WOX5 and a PLT protein, preferably WOX5 and PLT1. In addition the expression levels of further proteins can be altered, such as WIND1, SHR, SCR, RBR, PLT4 and PLT5 to regenerate a shoot from a plant cell. Preferably, the expression levels are transiently altered. The invention further pertains to a nucleic acid construct suitable for transient protein expression and the use of the protein combinations for regenerating a shoot from a plant cell.
Genetic modification of plants is hampered by the limited capacity of plant cells to regenerate. The current invention solves this problem by introducing or increasing the expression of a histidine kinase in a plant cell. Preferred histidine kinases are at least one of CHK2, CHK3 and CHK4.The invention therefore concerns a method for improving a cytokinin-induced regeneration capacity of a plant cell, wherein the method comprises a step of increasing or introducing the expression of a histidine kinase in the plant cell.The invention further pertains to a method for regenerating a plant, wherein the method comprises a step of introducing or increasing the expression of a histidine kinase and to a plant obtainable from such method. Moreover, the method concerns the use of at least one of CHK2, CHK3 and CHK4 for improving a cytokinin-induced regeneration capacity of a plant.
Cucumis meloCucumis melo, or powdery mildew-conferring part or variant thereof. The invention also relates to markers for identification of said QTLs, use thereof and methods for producing plants with increased resistance to powdery mildew and the plants thus obtained.
The present invention relates to a new method for increasing drought resistance of a plant. The method encompasses the impairment of the expression of a gene or genes in said plant. In comparison to a plant not manipulated to impair the expression of said gene(s), the plants display improved drought resistance. Also provided are plants and plant product that can be obtained by the method according to the invention.
The current invention pertains to a dicot plant cell, plant tissue or plant having a reduced expression of a wild type protein having at least 75% sequence identity with SEQ ID NO. 1. Furthermore, the invention pertains to a plant cell, plant tissue or plant comprising a modified protein and/or a truncated protein having at least about 75% sequence identity when calculated over the positions corresponding to positions 1 –66 and positions 78 –308 of SEQ ID NO: 1 and wherein the modified protein increases the resistance to Geminiviridae as compared to an otherwise identical plant cell, plant tissue or plant expressing the protein without the modification or without the truncation. The invention further pertains to nucleic acid constructs encoding the modified or truncated protein. In addition, the invention relates to methods for generating a plant cell, plant tissue or plant comprising the modified protein that increases the resistance to Geminiviridae.Furthermore, the invention pertains to a plant cell, plant tissue or plant obtainable by the method of the invention.
Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.
It was found that plants comprising modified CENPC protein comprising one or more active mutations which affect the functioning of CENPC protein yet allow plants expressing said modified CENPC protein to be viable, are able to induce haploid offspring after a cross to or with a wild type plant comprising a endogenous CENPC protein. The invention relates to generation of haploid and doubled haploid plants.
The invention pertains to the targeted alteration of a duplex DNA in a cell, whereby two site-specific nucleases generate an indel, such that the open reading frame is not altered after the second indel. The invention further pertains to the use of such nucleases for the targeted alteration of an open reading frame in duplex DNA and a kit of parts for use in a method of the invention. Using the method of the invention, novel plants were obtained having an improved herbicide resistance. The invention therefore also concerns plants having improved herbicide resistance due to the expression of an altered ALS protein.
It was found that plants with loss of functional Msi2 protein due to a nucleotide polymorphism resulting in the introduction of a premature stop codon in the Msi2 protein, are able to induce haploid offspring after a cross to or with a wild type plant comprising a functional Msi2 protein. The invention relates to generation of haploid and doubled haploid plants.
Stevia varieties with a high content of RebM, are disclosed Further provided are methods for producing Stevia plants having a high RebM content by negatively regulating certain genes selecting the resulting plants, and breeding with such plants to confer such desirable Reb M phenotypes to plant progeny.
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
C07H 1/00 - Procédés de préparation des dérivés du sucre
C07H 15/00 - Composés contenant des radicaux hydrocarbonés ou hydrocarbonés substitués, liés directement aux hétéro-atomes des radicaux saccharide
