The purpose of the present invention is to provide a highly versatile production method with which it is possible to use any hydrophobic peptide as the object of purification regardless of the type of amino acid residue at the N-terminal and to flexibly select a hydrophilic unit in accordance with the type of hydrophobic peptide when conducting HPLC purification by hydrophilizing a solid-phase-synthesized hydrophobic peptide using a hydrophilic unit. The present invention is a method for obtaining a purified peptide from a supported crude peptide configured from a support and a first peptide chain bonded on the C-terminal side to this support, wherein the method is characterized by having: a hydrophilization step A for obtaining a support-free hydrophilized peptide by introducing a linker and a hydrophilic unit in the stated order stepwise or in a single step to an amino group of a supported crude peptide; a support-cutting step B for cleaving the bond between the first peptide chain and the support at any step from before bonding the linker to the supported crude peptide until obtaining the hydrophilized peptide, or after having obtained a supported hydrophilized peptide; a chromatographic purification step for dissolving the support-free hydrophilized peptide obtained through the hydrophilization step A and the support-cutting step B in water, and treating the dissolved support-free hydrophilized peptide by liquid chromatography; and a linker-cutting step for cleaving the bond between the linker and the first peptide chain contained in the chromatographically purified support-free hydrophilized peptide by chemical treatment.
The present invention is generally directed to thiol quantitation assays, methods of performing the assays, and compounds used in the assays. It is more specifically directed to assays that include one or more disulfides and related molecules and methods. The disulfides contain a FRET pair.
G01N 33/52 - Utilisation de composés ou de compositions pour des recherches colorimétriques, spectrophotométriques ou fluorométriques, p. ex. utilisation de bandes de papier indicateur
The present invention is generally directed to thiol quantitation assays, methods of performing the assays, and compounds used in the assays. It is more specifically directed to assays that include one or more disulfides and related molecules and methods. The disulfides contain a FRET pair.
G01N 33/52 - Utilisation de composés ou de compositions pour des recherches colorimétriques, spectrophotométriques ou fluorométriques, p. ex. utilisation de bandes de papier indicateur
Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.
C07D 209/02 - Composés hétérocycliques contenant des cycles à cinq chaînons condensés avec d'autres cycles, ne comportant qu'un atome d'azote comme unique hétéro-atome du cycle condensés avec un carbocycle
C07D 209/56 - Systèmes cycliques contenant au moins trois cycles
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
The present invention is generally directed to biological assays. More specifically it is directed to optimal FRET pairs and methods related to their use. In a composition aspect, the present invention is directed to FRET-based protease substrates selected from the list of substrates shown in the Detailed Description. In a method aspect, the present invention is directed to a method of performing a FRET-based assay, where the assay includes the following steps: adding a solution or suspension containing one or more different proteins to a reaction vessel; adding a liquid comprising a FRET-substrate to the reaction vessel, wherein the FRET substrate is selected from the list of substrates shown in the Detailed Description. In a kit aspect, the present invention is directed to a kit for performing a FRET-based assay, wherein the kit comprises a protease substrate. The protease substrate is selected from the list of substrates shown in the Detailed Description.
C12Q 1/37 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase faisant intervenir une peptidase ou une protéinase
Methods for performing FRET-based biological assays using optimized FRET pairs, said methods comprise adding a solution or suspension containing one or more different proteins to a reaction vessel, adding a liquid comprising a FRET-based protease substrate to the reaction vessel Kit for performing a FRET-based assay, wherein the kit comprises a protease substrate.
Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.
C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
C07K 2/00 - Peptides à nombre indéterminé d'amino-acidesLeurs dérivés
C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques
Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
C07D 225/00 - Composés hétérocycliques contenant des cycles de plus de sept chaînons ne comportant qu'un atome d'azote comme unique hétéro-atome du cycle
C07D 245/00 - Composés hétérocycliques contenant des cycles de plus de sept chaînons comportant deux atomes d'azote comme uniques hétéro-atomes du cycle
C07D 209/02 - Composés hétérocycliques contenant des cycles à cinq chaînons condensés avec d'autres cycles, ne comportant qu'un atome d'azote comme unique hétéro-atome du cycle condensés avec un carbocycle
Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and the 3-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivate compounds having one or more benzo nitrogens.
C07D 209/02 - Composés hétérocycliques contenant des cycles à cinq chaînons condensés avec d'autres cycles, ne comportant qu'un atome d'azote comme unique hétéro-atome du cycle condensés avec un carbocycle
C07D 209/56 - Systèmes cycliques contenant au moins trois cycles
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Chemicals; namely, peptide and immunochemistry reagents used
in the bio-technology and pharmaceutical industries. Manufacture of peptides and antibodies to the specification
of others for academic and industrial research. Chemical and bio-chemical analysis, chemical laboratories
and chemical research.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Biomedical compounds, namely, monoclonal antibodies used in analyzing and detecting certain enzymes, receptors, oligonucleotides and proteins for in vitro scientific laboratory or research use in the biotechnology and pharmaceutical industries; biomedical compounds in the nature of amino acids, namely, peptides used in analyzing and detecting certain enzymes, receptors, oligonucleotides and proteins for laboratory or research use in the biotechnology and pharmaceutical industries; chemical reagents for non-medical purposes in the biotechnology and pharmaceutical industries Manufacture of peptides and antibodies, namely, synthetic peptides for pharmaceutical purposes and monoclonal antibodies for in vitro scientific or research use, to the specification of others for academic and industrial research Chemical and bio-chemical analysis, chemical laboratories and chemical research
15.
Cyanine dyes and their applications as luminescence quenching compounds
The quenching compounds of the invention are weakly luminescent cyanines that are substituted by one or more heteroaromatic quenching moieties. The quenching compounds of the invention exhibit little or no observable luminescence and efficiently quench a broad spectrum of luminescent compounds. The chemically reactive quenching compounds possess utility for labeling a wide variety of substances, including biomolecules. These labeled substances are highly useful for a variety of energy-transfer assays and applications.
C07D 209/46 - Iso-indolesIso-indoles hydrogénés avec un atome d'oxygène en position 1
C07D 209/02 - Composés hétérocycliques contenant des cycles à cinq chaînons condensés avec d'autres cycles, ne comportant qu'un atome d'azote comme unique hétéro-atome du cycle condensés avec un carbocycle
