Provided are a nucleic acid testing method, and a composition and kit for nucleic acid testing. According to the nucleic acid testing method, amplification testing of multiple targets can be performed simultaneously, and the amplification system does not require addition of an NFO endonuclease, so that the hybridization efficiency is greatly improved, and good testing sensitivity is thus achieved; moreover, a capture probe only complementarily matches a single-stranded product of a target sequence, ensuring the testing specificity. By means of the testing method, only a simple constant temperature device is required to achieve visual observability of nucleic acid testing results, and there is no need for a complex variable temperature instrument and a fluorescence detection device, so that operation is easy and convenient, results are convenient to read, no professional operation and result analysis are needed, and on-site and grassroots-level rapid nucleic acid testing is facilitated.
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
2.
TEST STRIP FOR DETECTING HUMAN CHORIONIC GONADOTROPIN IN SALIVA AND PREPARATION METHOD THEREOF
Provided is a test strip for detecting human chorionic gonadotropin (HCG) in saliva and a preparation method thereof, and belongs to the technical field of test strip detection. each 100 mL of the sample pad treatment solution includes the following components: 1.0 g to 1.5 g of Tris, 0.3 g to 0.8 g of casein, 50 μL to 100 μL of Tween-20, 0.5 g to 1.5 g of NaCl, 0.15 g to 0.2 g of dodecyltrimethylammonium bromide (DTAB), 0.8 g to 1.2 g of 4-nonylphenyl-polyethylene glycol, 3.5 g to 4.0 g of borax, 0.5 g to 1 g of polyvinylpyrrolidone (PVP), 3 g to 5 g of dithiothreitol (DTT), and 0.8 g to 2 g of L-cysteine. The test strip shows high sensitivity and desirable stability, can be stored at a room temperature for a long time and is not affected by the time and place of sample collection.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
A test strip for detecting human chorionic gonadotropin in saliva and a preparation method therefor. Calculated based on 100 mL, a sample pad treatment liquid comprises the following ingredients: 1.0-1.5 g of Tris, 0.3-0.8 g of casein, 50-100 μL of Tween-20, 0.5-1.5 g of NaCl, 0.15-0.2 g of dodecyltrimethylammonium bromide, 0.8-1.2 g of 4-nonylphenyl-polyethylene glycol, 3.5-4.0 g of borax, 0.5-1 g of polyvinylpyrrolidone, 3-5 g of dithiothreitol and 0.8-2 g of L-cysteine. The sample pad treatment liquid is used to treat a sample pad, so as to reduce the interference of other impurity ingredients in saliva, thereby achieving the effect of completely eliminating non-specific reactions. The test strip has high sensitivity and good stability, can be stored for a long time at room temperature, and has the advantages of being cleaner and more convenient, allowing testing to take place at any time, being unaffected by sample collection time and place, etc.
A chromatography-based multiplex nucleic acid detector, comprising: an air source device, wherein an air outlet end of the air source device is configured to be communicated with a first end of an amplification reagent tube; a heating device; a PCR pipeline; and a cover plate assembly mounted on the heating device, wherein an observation window is formed on the cover plate assembly, a PCR pipeline mounting position is formed on at least one of the cover plate assembly and the heating device, the PCR pipeline mounting position is configured to mount the PCR pipeline, and the heating device is configured to heat the PCR pipeline; the observation window is configured to mount a test strip; a second end of the amplification reagent tube is communicated with a first end of the PCR pipeline, and a second end of the PCR pipeline is configured to output an amplified sample to the test strip.
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
5.
PRIMER AND TEST KIT FOR DETECTING URINARY TRACT INFECTION PATHOGEN AND DRUG-RESISTANT GENE, AND USE THEREOF
The present application relates to the field of pathogen nucleic acid detection, and discloses a primer and a test kit for detecting a urinary tract infection pathogen and a drug-resistant gene, and a use thereof. In the present application, a detection reagent is fixed and freeze-dried in a strip of eight PCR tubes using an eight-strip freeze-drying technique. Four channels are used to detect four targets in each well, and the eight wells in the eight-tube strip are used to detect different targets. 31 targets and 1 internal standard gene are covered in total.
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
C12Q 1/6895 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les plantes, les champignons ou les algues
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
A two-dimensional multiplex chromatography test card is disclosed. The two-dimensional multiplex chromatography test card comprises a first absorbent pad (11), a first nitrocellulose membrane (10), a sample pad (1), a second nitrocellulose membrane (3) and a second absorbent pad (4) which are sequentially provided on a backing (5) in a lapping manner along a length direction of the backing (5). A first conjugating zone (9) on the sample pad (1) is coated with at least one first latex bead labeled antibody, and a second conjugating zone (2) on the sample pad (1) is coated with at least one second latex bead labeled antibody. The first nitrocellulose membrane (10) is provided with a first test line (12 and/or 13) and a first control line (14), and the first test line (12 and/or 13) is coated with a first antibody corresponding to the first latex bead labeled antibody. The second nitrocellulose membrane (3) is provided with a second test line (6 and/or 7) and a second control line (8).
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
G01N 33/577 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet faisant intervenir des anticorps monoclonaux
C07K 16/10 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant de virus de virus à ARN
G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
A portable ultrafast real-time fluorescent quantitative PCR instrument, comprising: a PCR reaction chip (1); a chip mounting module (2) used for mounting the PCR reaction chip (1); a temperature control floating module (3) comprising a temperature control device (301), an end of which is provided with a heating and refrigeration sheet (302) and a temperature sensor (303), wherein the heating and refrigeration sheet (302) is provided with a heating and refrigeration carrier plate (304), two sides of the heating and refrigeration carrier plate (304) are provided with thermal-insulation pressure plates (305), and the thermal-insulation pressure plates (305) are pressed against the chip mounting module (2), so that the PCR reaction chip (1) is placed at the heating and refrigeration carrier plate (304); and an optical module (4) comprising a switching device (401) and an in-place sensor (402). This PCR instrument is convenient to carry and transfer; and a single chip is used, so that the operation is simple, the sealing is reliable, a detection result can be quickly and stably obtained and the detection result is reliable, the PCR instrument can be applied to a variety of different detection objects, and has wider applicability.
A single-part rapid PCR chip, comprising: a chip base body (1). A reaction recess (2) is formed in the chip base body (1); a bottom film (3) is attached to the surface where the reaction recess (2) is located, and a reaction channel is formed by the bottom film and the reaction recess (2); a cylindrical sample loading hole (4) is formed in the chip base body (1); an opening in the bottom of the cylindrical sample loading hole (4) is communicated with one end of the reaction channel to form a sample loading channel (L1); an opening at an upper end of the hole wall of the cylindrical sample loading hole (4) extends by means of the hole wall and is communicated with the other end of the reaction channel to form an air outlet channel (L2); the cylindrical sample loading hole (4) is sealed by means of a sample loading hole cover (5); a press head (6) is provided at the position where the sample loading hole cover (5) corresponds to the cylindrical sample loading hole (4); and as the sample loading hole cover (5) is closed, the press head (6) presses a sample into the reaction channel. The single-part rapid PCR chip is designed in a flat channel structure, such that the sample can be rapidly heated and cooled, the temperature uniformity is better, and the accuracy is higher; by using a press type sample loading mode, excessive gas can be discharged, the sealing is reliable, and the risk of cross contamination is avoided.
tmaxtmaxtt≤0, PD control is used. In a temperature changing stage, a time optimal PID control algorithm and a traditional PID control algorithm are used for heating up or cooling down; in a constant temperature stage, the traditional PID control algorithm is used; and at a time point of switching of a variable temperature and a constant temperature, a temperature fast-setting control algorithm and an adaptive control algorithm are used. The control method and the control device have the advantages of precise temperature control, excellent temperature change benefits, fast temperature change, fast constant temperature and small overshoot, it can be ensured that a target value is reached at the fastest speed, the time required for a temperature rising stage and a temperature falling stage is shortened, and oscillation and overshoot of temperature waveforms of PCR in a circulation section are reduced.
G05B 11/42 - Commandes automatiques électriques avec les dispositions nécessaires pour obtenir des caractéristiques particulières, p. ex. proportionnelles, intégrales, différentielles pour obtenir une caractéristique à la fois proportionnelle et dépendante du temps, p. ex. P.I., P.I.D.
10.
SWAB FLOCKING DEVICE AND SWAB BLOWING AND FLOCKING PROCESS
A swab flocking device and a swab blowing and flocking process. A conveying drag chain (4) transversely penetrates through a flocking box (1), a plurality of swab hangers (5) are arranged on the conveying drag chain (4) at intervals, and the conveying drag chain (4) is connected to a first motor (9). A plurality of fans (7, 8) are arranged on the outer side of the conveying drag chain (4) in the flocking box (1). During flocking, a swab rod (20) is hung on the swab hanger (5) and is conveyed forwards by the conveying drag chain (4), and the plurality of fans (7, 8) blow fluffs to the end of the swab rod (20). Flocking is performed on the swab rod (20) in a blowing mode. The fluffs are uniformly attached to the surface of the swab rod (20), the flocking effect is good, and the flocking process only needs to provide wind power by means of the fans (7, 8). Compared with the electrostatic flocking mode, the demand of electric energy is greatly reduced, the power consumption is reduced, the energy consumption is reduced, the electrostatic effect in the flocking process is also avoided, the flocking effect is ensured, and the quality of the swab is ensured.
B05C 19/00 - Appareillages spécialement adaptés pour appliquer des matériaux en particules à des surfaces
B05C 19/06 - Stockage, alimentation ou régulation de l'application du matériau en particulesRécupération du matériau en particules en excès
B05D 3/14 - Traitement préalable des surfaces sur lesquelles des liquides ou d'autres matériaux fluides doivent être appliquésTraitement ultérieur des revêtements appliqués, p. ex. traitement intermédiaire d'un revêtement déjà appliqué, pour préparer les applications ultérieures de liquides ou d'autres matériaux fluides par des moyens électriques
B05C 13/02 - Moyens pour manipuler ou tenir des objets, p. ex. des objets individuels pour des objets particuliers
11.
Swab flocking device, and flock blowing and flocking process for swab
Disclosed are a swab flocking device and a flock blowing and flocking process for a swab. A conveying drag chain transversely penetrates through a flocking box, several swab hangers are arranged on the conveying drag chain at intervals, and the conveying drag chain is connected to a first motor. Several blowers are arranged on an outer side of the conveying drag chain in the flocking box. During flocking, a swab stick is hung on the swab hanger and then is conveyed forwards by the conveying drag chain, and several blowers are configured to blow flocks onto an end head of the swab stick.
B05D 3/14 - Traitement préalable des surfaces sur lesquelles des liquides ou d'autres matériaux fluides doivent être appliquésTraitement ultérieur des revêtements appliqués, p. ex. traitement intermédiaire d'un revêtement déjà appliqué, pour préparer les applications ultérieures de liquides ou d'autres matériaux fluides par des moyens électriques
Orthopedic articles; radiological apparatus for medical
purposes; dental apparatus and instruments; analysers for
bacterial identification for medical purposes; apparatus for
use in medical analysis; diagnostic apparatus for medical
purposes; medical apparatus and instruments; massage
apparatus; surgical apparatus and instruments.
Massage apparatus; Orthopedic articles. Advertising; Presentation of goods on communication media, for retail purposes; Commercial administration of the licensing of the goods and services of others; Organization of exhibitions for commercial or advertising purposes; Import-export agency services; Sales promotion for others; Procurement services for others [purchasing goods and services for other businesses]; Personnel management consultancy; Systemization of information into computer databases.
