Abrégé

An identity recognition method, an apparatus, a computer device, and a storage medium are provided. The method includes: obtaining a signal to be identified which includes a millimeter wave signal and an audio signal; performing living feature detection based on the millimeter wave signal and the audio signal, and acquiring a living millimeter wave signal and a living audio signal; performing feature fusion of the living millimeter wave signal and the living audio signal, and acquiring a fusion response diagram of a living voice signal; and performing identity recognition based on the fusion response diagram of the living voice signal.

Classes IPC  ?

• G10L 17/10 - Systèmes multimodaux, c.-à-d. basés sur l’intégration de moteurs multiples de reconnaissance ou de fusion de systèmes experts
• G01S 13/32 - Systèmes pour mesurer la distance uniquement utilisant la transmission d'ondes continues, soit modulées en amplitude, en fréquence ou en phase, soit non modulées
• G10L 17/02 - Opérations de prétraitement, p. ex. sélection de segmentReprésentation ou modélisation de motifs, p. ex. fondée sur l’analyse linéaire discriminante [LDA] ou les composantes principalesSélection ou extraction des caractéristiques
• G10L 17/20 - Transformations de motifs ou opérations ayant pout but d’augmenter la robustesse du système, p. ex. contre le bruit du canal ou les différentes conditions de fonctionnement
• G10L 21/0232 - Traitement dans le domaine fréquentiel
• G10L 25/78 - Détection de la présence ou de l’absence de signaux de voix

Abrégé

A spatial location precision stabilization system with dual level multi-degree of freedom is provided. The system includes a motion platform; a second-level platform connected to the motion platform and located below the motion platform, the second-level platform is provided with a second electromagnetic driving component to adjust a position of the motion platform; a first-level platform connected to the second-level platform and located below the second-level platform, the first-level platform is provided with a first electromagnetic driving component to adjust the second-level platform, so as to adjust the position of the motion platform; and a controller configured to control the first electromagnetic driving component and the second electromagnetic driving component.

Classes IPC  ?

• F16M 11/18 - Têtes des supports avec mécanisme déplaçant les appareils par rapport au banc

Abrégé

The present invention discloses a method for designing an interference noise of speech based on the human speech structure, including the following steps: (1): obtaining a large amount of speech data containing different speakers and different speech contents, extracting voiceprint information, and then building an initial speech data set; (2): for each user, obtaining a small amount of speech data of the user, extracting voiceprint information, and then matching the most similar speech data in the initial speech data set; (3): performing data augmentation on the matched speech data; (4): segmenting the augmented speech data with a phoneme segmentation algorithm to form a vowel data set and a consonant data set; (5): constructing three noise sequences based on the vowel data set and the consonant data set, and performing superimposition to obtain an interference noise; and (6): continuously generating and playing randomly generated interference noise, and continuously injecting the interference noise into recordings to implement continuous interference. With the present invention, the interference noise cannot be removed from the speech, thereby avoiding the leakage of user privacy information.

Classes IPC  ?

• G10L 17/14 - Par catégorisation phonémique ou reconnaissance de la parole avant identification ou vérification du locuteur
• G10L 21/0272 - Séparation du signal de voix

Abrégé

A transmission electron microscope sample rod (100) and a sample testing method. The transmission electron microscope sample rod (100) comprises a heating element (10) and a testing mechanism (20), wherein the heating element (10) is configured to heat a sample, the testing mechanism (20) is constructed with a plurality of connecting arms (21) which are arranged at intervals in a circumferential direction of the testing mechanism (20), an accommodating space (23) is defined by means of the plurality of connecting arms (21), one side of each connecting arm (21) facing the accommodating space is defined as an output end (202), the plurality of output ends (202) jointly clamp the sample, each connecting arm (21) can extend and retract in an extension direction of the output end (202), and each connecting arm (21) is connected to the heating element (10).

Classes IPC  ?

• G01N 23/04 - Recherche ou analyse des matériaux par l'utilisation de rayonnement [ondes ou particules], p. ex. rayons X ou neutrons, non couvertes par les groupes , ou en transmettant la radiation à travers le matériau et formant des images des matériaux
• H01J 37/20 - Moyens de support ou de mise en position de l'objet ou du matériauMoyens de réglage de diaphragmes ou de lentilles associées au support
• G01Q 30/20 - Dispositifs ou procédés de manipulation d'échantillons

Abrégé

A liquid core waveguide photoreactor and a liquid core waveguide illumination method. The difference between the refractive index of a channel wall and the refractive index of a medium on the outer side of the channel wall is used, and an included angle between light and the channel wall is set, such that light transmission meets a total-reflection condition; and multiple total-reflection transmissions are performed by using a medium in the channel as a light guide medium, such that a light guide method similar to an optical fiber is implemented. The liquid core waveguide illumination method allows light to repeatedly run through an inner channel (2) multiple times in a longitudinal direction, and thus strong and uniform illumination can both be maintained in a flow photoreactor having a length of up to several centimeters, such that the length of the effective illumination received by a photocatalytic organic reaction sample in the channel is improved.

Classes IPC  ?

• B01J 19/12 - Procédés utilisant l'application directe de l'énergie ondulatoire ou électrique, ou un rayonnement particulaireAppareils à cet usage utilisant des radiations électromagnétiques
• B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en généralAppareils appropriés

Abrégé

The present application relates to a circuit and method for generating a labeled image. The circuit for generating a labeled image comprises: a plurality of pixels arranged in an array, wherein the pixels each comprise a photosensitive structure and a voltage reader, the photosensitive structure is provided with a photoelectric sensing area and an equivalent capacitance area, and the voltage reader is electrically connected to the equivalent capacitance area; a dual-correlation sampler, wherein a first input end of the dual-correlation sampler is electrically connected to the voltage readers, and a second input end of the dual-correlation sampler is used for allowing a threshold value voltage to access same; a counter, wherein a counting control end of the counter is electrically connected to an output end of the dual-correlation sampler; and a processor, which is electrically connected to the counter, wherein the processor is used for forming a label. The circuit for generating a labeled image is based on physically unclonable features of photosensitive structures, thereby facilitating the attachment of reliable and accurate labels to images.

Classes IPC  ?

• H04L 9/32 - Dispositions pour les communications secrètes ou protégéesProtocoles réseaux de sécurité comprenant des moyens pour vérifier l'identité ou l'autorisation d'un utilisateur du système
• G06F 21/30 - Authentification, c.-à-d. détermination de l’identité ou de l’habilitation des responsables de la sécurité
• H04N 25/00 - Circuits de capteurs d'images à l'état solide [capteurs SSIS]Leur commande

Abrégé

Disclosed in the present invention is a soil organic matter prediction method based on nonlinear memory-based learning and spectroscopy, comprising: constructing a sample set on the basis of the real soil organic matter content and spectral data of each soil sample, and dividing the sample set into a training set and a validation set; from the training set, screening for k samples having spectral data most similar to that of each soil sample to be tested to construct a training subset, and fitting a random forest model on the basis of the training subset and by means of ten-fold cross validation to obtain a nonlinear memory-based learning model; verifying the prediction accuracy of the nonlinear memory-based learning model by means of the validation set, when the prediction accuracy reaches a standard, a final nonlinear memory-based learning model being obtained; and inputting the spectral data of the soil sample to be tested into the corresponding final nonlinear memory-based learning model to obtain a predicted value of soil organic matter. According to the method, corresponding soil organic matter contents can be accurately obtained on the basis of spectral data of different soil samples to be tested. Also disclosed in the present invention is a soil organic matter prediction apparatus based on nonlinear memory-based learning and spectroscopy.

Classes IPC  ?

• G06Q 10/04 - Prévision ou optimisation spécialement adaptées à des fins administratives ou de gestion, p. ex. programmation linéaire ou "problème d’optimisation des stocks"

Abrégé

Disclosed in the present invention are a soil organic carbon prediction method and apparatus based on spectrum-guided ensemble learning. The method comprises: obtaining soil samples and true organic carbon content and original soil spectra thereof, preprocessing the original soil spectra to obtain soil spectrum samples, constructing a sample set on the basis of the soil spectrum samples, dividing the sample set into a first training set and a validation set, and labelling with the true organic carbon content; training a partial least squares regression model, a Cubist model and a random forest model on the basis of the first training set and the corresponding label, and obtaining a carbon content prediction value set of the three models; and constructing a second training set on the basis of the carbon content prediction value set of the three models and soil spectrum principal component data, and training a second random forest model by means of the second training set and the corresponding label to obtain a spectrum-guided ensemble model. According to the method, the advantages of different prediction models are comprehensively utilized, and the carbon content of soil can be accurately predicted.

Classes IPC  ?

• G01N 21/27 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en utilisant la détection photo-électrique
• G01N 21/01 - Dispositions ou appareils pour faciliter la recherche optique

Abrégé

An information retrieval method and apparatus, a computer device, and a storage medium are provided. The method includes receiving a request ciphertext sent by a client, the request ciphertext including an encrypted first code, the first code being generated based on a request entry of the client and a preset encoding algorithm; performing distributed matching on a plurality of candidate codes based on the request ciphertext to determine a second code matching the request ciphertext, the candidate codes being generated based on index entries of a database and the preset encoding algorithm; and sending a request information ciphertext associated with the index entry corresponding to the second code to the client.

Classes IPC  ?

• H04L 9/06 - Dispositions pour les communications secrètes ou protégéesProtocoles réseaux de sécurité l'appareil de chiffrement utilisant des registres à décalage ou des mémoires pour le codage par blocs, p. ex. système DES

Abrégé

The present invention relates to the field of ultra-precision cutting technology, specifically to a ultra-precision cutting quasi-static force measurement system based on piezoelectric ceramic sensor. This system includes a piezoelectric ceramic force sensing unit that responds to the force applied by a single-point diamond tool and generates an electric charge signal sent to an external post-processing module. The post-processing module includes a preamplifier circuit for the charge, a low-pass filter circuit, an ADC (Analog-to-Digital Converter) module, a DSP (Digital Signal Processor) and a computer. The computer calculates the actual force Fi applied to the piezoelectric ceramic force sensor at moment i based on the solution of the dynamically changing force fi at each moment and the accumulation of the dynamically changing forces from previous moment.

Classes IPC  ?

• G01L 1/16 - Mesure des forces ou des contraintes, en général en utilisant les propriétés des dispositifs piézo-électriques
• B23Q 17/09 - Agencements sur les machines-outils pour indiquer ou mesurer pour indiquer ou mesurer la pression de coupe ou l'état de l'outil de coupe, p. ex. aptitude à la coupe, charge sur l'outil
• G01L 5/00 - Appareils ou procédés pour la mesure des forces, du travail, de la puissance mécanique ou du couple, spécialement adaptés à des fins spécifiques

Abrégé

An information retrieval method and apparatus (100), and a computer device (200) and a storage medium. The method comprises: receiving a request ciphertext sent by a client, wherein the request ciphertext comprises an encrypted first code, and the first code is generated on the basis of a request item of the client and a preset coding algorithm; performing distributed matching on a plurality of candidate codes on the basis of the request ciphertext, so as to determine a second code matching the request ciphertext, wherein the candidate codes are generated on the basis of an index item of a database and the preset coding algorithm; and sending to the client a request information ciphertext under the index item corresponding to the second code.

Classes IPC  ?

• G06F 16/2455 - Exécution des requêtes
• G06F 21/60 - Protection de données

Abrégé

Provided are a method for expressing a D-amino acid oxidase and the use thereof. A wild type D-amino acid oxidase having catalytic activity on catalyzing a substrate, DL-glufosinate-ammonium, is obtained by screening; and the activity and soluble expression of the D-amino acid oxidase obtained by host expression are further improved by means of expression host screening, addition of molecular chaperones and the like. The present invention achieves kinetic resolution of racemic glufosinate-ammonium, and can be used for production of L-glufosinate-ammonium.

Classes IPC  ?

• C12N 15/53 - Oxydoréductases (1)
• C12N 9/06 - Oxydoréductases (1.), p. ex. luciférase agissant sur des composés contenant de l'azote comme donneurs (1.4, 1.5, 1.7)
• C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12P 7/40 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carboxyle
• C12R 1/19 - Escherichia coli

Abrégé

Disclosed in the present invention are a template-based Chinese privacy policy summary generation method and apparatus. The method comprises: acquiring a Chinese privacy policy text, and dividing same into clause statements; performing identification and classification on the clause statements by using an identification model for an important privacy clause and a classification model for an important privacy clause, so as to obtain important privacy clause statements and categories corresponding thereto; according to the categories of the important privacy clause statements, retrieving and matching privacy clause interpretation templates for the important privacy clause statements; performing translation computation on the important privacy clause statements and the matched privacy clause interpretation templates thereof by using a privacy policy interpretation model, so as to obtain translations corresponding to the important privacy clause statements; and summarizing the translations of the important privacy clause statements according to the categories of the important privacy clause statements, so as to generate a privacy policy summary. By means of the method and apparatus, a Chinese privacy policy summary, which is popular and easy to understand, can be generated.

Classes IPC  ?

• G06F 16/34 - NavigationVisualisation à cet effet

Abrégé

The present invention discloses a perceptual representation learning method for protein conformations based on a pre-trained language model, including: obtaining a protein made up of an amino acid sequence, building different data sets according to protein conformations, and defining a prompt for each type of protein conformation; building, based on a pre-trained language model, a representation learning module for fusing an embedding representation of each type of the prompt into an embedding representation of the protein, so as to obtain a protein embedding representation under a prompt identifier; building a task module for performing task prediction on a task corresponding to each type of protein conformation based on the protein embedding representation under the prompt identifier; building a loss function for each type of task based on a task prediction result and a tag, and updating model parameters of the representation learning module and the task module in combination with loss functions of all types of tasks and the different data sets; and after the model parameters are updated, extracting the representation learning module as a protein representation module. Protein representations under different conformations can be obtained by the method.

Classes IPC  ?

• G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
• G16B 5/00 - TIC spécialement adaptées à la modélisation ou aux simulations dans la biologie des systèmes, p. ex. réseaux de régulation génétique, réseaux d’interaction entre protéines ou réseaux métaboliques

Abrégé

An antistatic composition and an olefin polymerization method are provided. The antistatic composition includes: an oil-soluble hyperbranched polyamidoamine, an oil-soluble surfactant and an aliphatic hydrocarbon, wherein the oil-soluble hyperbranched polyamidoamine has a molecular weight with a range of 5000 to 40000. The olefin polymerization method includes: adding an olefin, a catalyst and the antistatic composition into a polymerization reactor to undergo a polymerization to obtain a polyolefin.

Classes IPC  ?

• C08K 5/17 - AminesComposés d'ammonium quaternaire
• C08F 4/00 - Catalyseurs de polymérisation
• C08F 10/00 - Homopolymères ou copolymères d'hydrocarbures aliphatiques non saturés contenant une seule liaison double carbone-carbone
• C08K 5/42 - Acides sulfoniquesLeurs dérivés

Abrégé

A method and an apparatus for privacy protection biometric authentication and an electronic device. The method comprises the following steps: constructing a corresponding a biological data template according to a biological information data set input by a user when registering; generating a pair of public key and private key by asymmetric cryptography technology; generating encrypted biological data by a secret sharing solution and OKVS technology according to the biological data template and the private key; sending the public key and the encrypted biological data to a server; recovering the private key by OKVS technology according to the biological data input by the user during authentication and the encrypted biological data; constructing a signature according to the recovered private key and the corresponding public key; sending the signature to the server, so that the server verifies the user according to the public key and the signature.

Classes IPC  ?

• H04L 9/40 - Protocoles réseaux de sécurité
• H04L 9/08 - Répartition de clés
• H04L 9/32 - Dispositions pour les communications secrètes ou protégéesProtocoles réseaux de sécurité comprenant des moyens pour vérifier l'identité ou l'autorisation d'un utilisateur du système

Abrégé

An antistatic composition and an olefin polymerization method. The antistatic composition comprises: an oil-soluble hyperbranched polyamide-amine, an oil-soluble surfactant and an aliphatic hydrocarbon, the molecular weight of the oil-soluble hyperbranched polyamide-amine being 5,000-40,000. The olefin polymerization method comprises: adding an olefin, a catalyst and the antistatic composition to a polymerization reactor, and carrying out a polymerization reaction to obtain a polyolefin.

Classes IPC  ?

• C08K 5/01 - Hydrocarbures
• C08K 5/42 - Acides sulfoniquesLeurs dérivés
• C08K 5/544 - Composés contenant du silicium contenant de l'azote
• C08K 5/17 - AminesComposés d'ammonium quaternaire
• C08L 79/02 - Polyamines
• C08F 110/02 - Éthylène
• C08F 2/48 - Polymérisation amorcée par énergie ondulatoire ou par rayonnement corpusculaire par la lumière ultraviolette ou visible

Abrégé

Disclosed in the present invention is an olefin polymerization reaction kinetic parameter estimation method. The method comprises: E1, determining single-active-site reaction kinetic parameters; E2, determining the number of active sites and multi-active-site reaction kinetic parameters; and E3, outputting reaction kinetic parameters corresponding to all active sites. E1 comprises: selecting a suitable polymerization reaction mechanism and research method; acquiring experimental conditions and pre-exponential factor, activation energy and reference temperature initial values of the Arrhenius equation; establishing a single-active-site reaction kinetic model on the basis of said method and data; and on the basis of experimental actual measurement data and the single-active-site reaction kinetic model, obtaining polymerization reaction kinetic parameters and a target function value by means of a Bayesian optimization algorithm. E2 comprises: assuming that the number of active sites is the number of active sites of the previous calculation step plus one, establishing a multi-active-site reaction kinetic model; and on the basis of experimental actual measurement data and the multi-active-site reaction kinetic model, obtaining polymerization reaction kinetic parameters and a target function value by means of the Bayesian algorithm.

Classes IPC  ?

• G16C 60/00 - Science informatique des matériaux, c.-à-d. TIC spécialement adaptées à la recherche des propriétés physiques ou chimiques de matériaux ou de phénomènes associés à leur conception, synthèse, traitement, caractérisation ou utilisation
• C08F 210/02 - Éthylène
• G06F 30/20 - Optimisation, vérification ou simulation de l’objet conçu

Abrégé

Provided is use of a tumor cell-derived exosome in promoting the growth of tumor cells or tumor organoids in vitro, which relates to the technical field of biology. The exosome is added to a tumor cell-like culture medium, increasing the growth speed of the tumor cells.

Classes IPC  ?

• C12N 5/09 - Cellules tumorales
• C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains

Abrégé

A cell-penetrating peptide and the use thereof. The cell-penetrating peptide is named as cell-penetrating peptide GAN_839K, the amino acid sequence thereof being RLWRIWHQNRRMRYRK. The cell-penetrating peptide can penetrate cell membranes of various types of cells to enter the cells, has the function of delivering proteins into cells, and has no toxicity to cells. The described small peptide can be applied to development of drug delivery systems, preparation of nano materials, etc.

Classes IPC  ?

• C07K 7/08 - Peptides linéaires ne contenant que des liaisons peptidiques normales ayant de 12 à 20 amino-acides
• A61K 47/64 - Conjugués médicament-peptide, médicament-protéine ou médicament-acide polyaminé, c.-à-d. l’agent de modification étant un peptide, une protéine ou un acide polyaminé lié par covalence ou complexé à un agent thérapeutiquement actif

Abrégé

Provided are a (2R,3R)-butanediol dehydrogenase mutant with improved thermal stability and use thereof. By means of site-directed mutagenesis modification of a BS-BDH sequence, the thermal stability thereof is improved, and compared with a wild type, 21 single-point mutants have improved thermal stability. On the basis of single-point mutants, double-point mutants are constructed, and the thermal stability of the double-point mutants is further improved. The described method effectively improves the thermal stability of the (2R,3R)-butanediol dehydrogenase derived from Bacillus subtilis, which is beneficial to alleviating the dilemma that industrial application cannot be achieved due to the poor thermal stability of (2R,3R)-butanediol dehydrogenase, thereby promoting the biocatalytic synthesis of acetoin.

Classes IPC  ?

• C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
• C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
• C12N 15/53 - Oxydoréductases (1)
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12P 7/18 - Polyols
• C12P 7/26 - Cétones

Abrégé

An active disturbance rejection control method and system (1000) based on an error compensation expansion state observer. The method comprises: a linear active disturbance rejection controller obtaining an input signal and each output state of an expansion state observer, and outputting a first control signal; constructing an expansion state observer state space model for output displacement, converting a controlled object into an integrator series type, and based on the controlled object, obtaining an output displacement signal; inputting the output displacement signal and a control input signal into the expansion state observer, and obtaining each output state; and feeding back into the linear active disturbance rejection controller and the state space model.

Classes IPC  ?

• G05B 13/02 - Systèmes de commande adaptatifs, c.-à-d. systèmes se réglant eux-mêmes automatiquement pour obtenir un rendement optimal suivant un critère prédéterminé électriques

Abrégé

The present invention relates to the field of bioengineering, and specifically, to a recombinant bionic polypeptide nanomaterial for the precise modulation of macrophage polarization and use thereof. According to the present invention, the prepared recombinant bionic polypeptide is capable of self-assembly into a nanofiber. The recombinant bionic polypeptide nanomaterial formed by the self-assembly can specifically activate the macrophage M1 polarization. Such polarized macrophages exhibit killing effects against tumor cells, suggesting good prospects for the present invention in tumor immunotherapy.

Classes IPC  ?

• C07K 19/00 - Peptides hybrides
• A61K 38/16 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés
• A61P 35/00 - Agents anticancéreux

Abrégé

Provided are a 2,3-butanediol dehydrogenase mutant and use thereof. Specifically, an SmBDH sequence is subjected to site-directed mutagenesis modification, so that the oxidoreductase activity of the sequence is increased. Compared with a wild type, the most significant increase in enzyme activity is observed in a D245C mutant, which has an oxidase activity 7.64 times that of the wild type and a reductase activity 4.42 times that of the wild type. The provided 1,3-butanediol dehydrogenase mutant is beneficial for solving the problem of low efficiency in enzymatic synthesis of 2,3-butanediol and acetoin, so that the problems of high cost, serious pollution, tedious steps and the like caused by a chemical synthesis method are alleviated, and industrial application of biosynthesis of 2,3-butanediol and acetoin is facilitated.

Classes IPC  ?

• C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
• C12N 15/53 - Oxydoréductases (1)
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12P 7/26 - Cétones
• C12R 1/125 - Bacillus subtilis

Abrégé

Provided are a genetically engineered bacterium capable of improving the secretion of an endogenous enzyme, a construction method therefor, and use thereof. Taking polygalacturonase, an endogenous enzyme, as a starting point, by carrying out a transcriptome sequencing analysis on a normal bacterial strain and a bacterial strain with the polygalacturonase knocked out, a key gene Km_10019 related to endogenous enzyme secretion is selected by screening, which is experimentally verified to regulate the extracellular secretion of the endogenous enzyme. Genetic engineering modification is carried out on Kluyveromyces marxianus to obtain a genetically engineered bacterium overexpressing Km_10019, and the amount of polygalacturonase secreted by the bacterial strain is 1.7 times that secreted by a control, which is of great significance for the development of protease preparations.

Classes IPC  ?

• C12N 15/81 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons pour levures
• C12N 9/24 - Hydrolases (3.) agissant sur les composés glycosyliques (3.2)
• C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
• C12N 1/19 - LevuresLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12R 1/645 - Champignons

Abrégé

Provided are a genetically engineered bacterium utilizing pectin, a construction method therefor, and use thereof, which belong to the technical field of genetic engineering. Kluyveromyces marxianus is used as a starting bacterium for the genetically engineered bacterium, and the genetically engineered bacterium has a polygalacturonase overexpression module. Genetic engineering modification is carried out on the Kluyveromyces marxianus to obtain the genetically engineered bacterium overexpressing polygalacturonase, and the pectin is completely hydrolyzed to release a carbon source available to the bacterium, such that the modified genetically engineered bacterium can continue to grow by using a hydrolysate as the carbon source. On the basis of the characteristic that the Kluyveromyces marxianus has efficient pectinase expression and secretion in an SD culture medium, by optimizing culture conditions, low-energy-consumption and high-added-value processing is carried out on fruit processing waste and pectin in discharged water by utilizing the genetically engineered bacterium.

Classes IPC  ?

• C12N 1/19 - LevuresLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 15/56 - Hydrolases (3) agissant sur les composés glycosyliques (3.2), p. ex. amylase, galactosidase, lysozyme
• C12N 15/81 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons pour levures
• C12P 7/22 - Préparation de composés organiques contenant de l'oxygène contenant un groupe hydroxyle aromatiques
• C12P 7/62 - Esters d’acides carboxyliques
• C12P 19/14 - Préparation de composés contenant des radicaux saccharide préparés par action d'une carbohydrase, p. ex. par action de l'alpha-amylase
• C02F 3/34 - Traitement biologique de l'eau, des eaux résiduaires ou des eaux d'égout caractérisé par les micro-organismes utilisés
• B09B 3/60 - Traitement biochimique, p. ex. en utilisant des enzymes
• C12R 1/645 - Champignons

Abrégé

Provided are an alcohol dehydrogenase mutant and use. The alcohol dehydrogenase mutant is obtained by mutating asparagine at position 258 of the amino acid sequence set forth in SEQ ID NO. 1 into cysteine. A key amino acid residue affecting the activity of alcohol dehydrogenase is found; on the basis of (2S, 3S)-2,3-butanol dehydrogenase (BDH, NCBI accession No.: WP_211439413) derived from Corynebacterium ghutamicum, by means of a rationally designed molecular modification method, the problem that the wild-type enzyme has no enzyme activity or low enzyme activity for natural or non-natural substrates is solved. The resulting mutant can catalyze the preparation of chiral diols with optical purity, i.e., (2S, 5S)-2,5-hexanediol and (2S,3S)-2,3-butanediol, thereby providing application prospects for producing pharmaceutical precursors (2S,5S)-2,5-hexanediol and (2S,3S)-2,3-butanediol by biotransformation.

Classes IPC  ?

• C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
• C12N 15/53 - Oxydoréductases (1)
• C12P 7/18 - Polyols
• C12P 7/26 - Cétones

Abrégé

Disclosed in the present invention is a method for synthesizing a primary intermediate of a hair dye. The method comprises: starting from a 2-hydroxy-5-nitroacetophenone compound, and subjecting same to carbonyl reduction and nitro reduction, Fmoc protection of amino, intramolecular cyclization, coupling, and deprotection under mild conditions, so as to obtain the corresponding primary intermediate of a hair dye. The synthesis method of the present invention does not require the use of raw materials such as p-nitrophenol and strongly corrosive concentrated sulfuric acid which are explosion hazards, and has high levels of safety. In the synthesis method of the present invention, six-membered cyclic carbonate is used as an important coupling intermediate, thereby avoiding the use of equivalent azodicarboxylate diester and triphenylphosphine and the generation of a byproduct, i.e., triphenylphosphine oxide, which is difficult to carry out post-treatment on. The method for synthesizing a primary intermediate of a hair dye of the present invention has mild reaction conditions, avoids a tedious multi-step protection/deprotection process, has simple steps, and is beneficial for industrial production.

Classes IPC  ?

• C07C 213/08 - Préparation de composés contenant des groupes amino et hydroxy, amino et hydroxy éthérifiés ou amino et hydroxy estérifiés liés au même squelette carboné par des réactions n'impliquant pas la formation de groupes amino, de groupes hydroxy ou de groupes hydroxy éthérifiés ou estérifiés
• C07C 217/76 - Composés contenant des groupes amino et hydroxy éthérifiés liés au même squelette carboné ayant des groupes amino liés à des atomes de carbone de cycles aromatiques à six chaînons et des groupes hydroxy éthérifiés liés à des atomes de carbone acycliques ou à des atomes de carbone de cycles autres que des cycles aromatiques à six chaînons du même squelette carboné
• C07C 269/06 - Préparation de dérivés d'acide carbamique, c.-à-d. de composés contenant l'un des groupes l'atome d'azote ne faisant pas partie de groupes nitro ou nitroso par des réactions n'impliquant pas la formation de groupes carbamate
• C07C 271/28 - Esters des acides carbamiques ayant des atomes d'oxygène de groupes carbamate liés à des atomes de carbone acycliques avec l'atome d'azote d'au moins un des groupes carbamate lié à un atome de carbone d'un cycle aromatique à six chaînons à un atome de carbone d'un cycle aromatique à six chaînons non condensé
• C07D 319/08 - Dioxanes-1, 3Dioxanes-1, 3 hydrogénés condensés avec des carbocycles ou avec des systèmes carbocycliques
• C07C 269/04 - Préparation de dérivés d'acide carbamique, c.-à-d. de composés contenant l'un des groupes l'atome d'azote ne faisant pas partie de groupes nitro ou nitroso à partir d'amines avec formation de groupes carbamate
• C07C 213/02 - Préparation de composés contenant des groupes amino et hydroxy, amino et hydroxy éthérifiés ou amino et hydroxy estérifiés liés au même squelette carboné par des réactions impliquant la formation de groupes amino à partir de composés contenant des groupes hydroxy ou des groupes hydroxy éthérifiés ou estérifiés
• C07C 215/78 - Composés contenant des groupes amino et hydroxy liés au même squelette carboné ayant des groupes hydroxy et des groupes amino liés à des atomes de carbone de cycles aromatiques à six chaînons du même squelette carboné du même cycle aromatique à six chaînons non condensé contenant au moins deux groupes hydroxy liés au squelette carboné
• C07D 209/14 - Radicaux substitués par des atomes d'azote ne faisant pas partie d'un radical nitro

Abrégé

A human speech structure-based speech interference noise design method, comprising the following steps: (1) acquiring a large amount of speech data comprising different speakers and different speaking content, extracting voiceprint information and then constructing an initial speech data set; (2) for each user, acquiring a small amount of user speech data, extracting voiceprint information and then matching the closest speech data in the initial speech data set; (3) performing data augmentation on the matching speech data; (4) segmenting the augmented speech data by using a phoneme segmentation algorithm to form a vowel data set and a consonant data set; (5) constructing three noise sequences on the basis of the vowel data set and the consonant data set, and superposing the three noise sequences to obtain interference noise; and (6) continuously generating random interference noise and playing back the random interference noise, and injecting continuous interference noise into an audio record to realize continuous interference. According to the present invention, interference noise cannot be removed from speech, so that leakage of privacy information of a user is avoided.

Classes IPC  ?

• G10L 25/48 - Techniques d'analyse de la parole ou de la voix qui ne se limitent pas à un seul des groupes spécialement adaptées pour un usage particulier
• G10L 25/03 - Techniques d'analyse de la parole ou de la voix qui ne se limitent pas à un seul des groupes caractérisées par le type de paramètres extraits
• G10L 17/18 - Réseaux neuronaux artificielsApproches connexionnistes

Abrégé

The present invention relates to the technical field of enzyme engineering, and in particular, to a rare earth-dependent alcohol dehydrogenase mutant and use thereof. According to the present invention, a method combining the MutCompute design tool based on deep learning and a FoldX tool based on an energy function is adopted to determine a to-be-mutated site of a rare earth-dependent alcohol dehydrogenase PedH. Mutants with an increase of 130% to 260% in the activity for methanol, ethanol, or 5-hydroxymethylfurfural were obtained by means of screening, among which two mutants also demonstrated improved stability. The present invention has important prospects in the construction of artificial methylotrophic bacteria and the production of important compounds such as FDCA in an environment-friendly manner, and solves the contradiction between stability and activity of enzymes.

Classes IPC  ?

• C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
• C12N 15/53 - Oxydoréductases (1)
• C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
• C12P 17/04 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle contenant un hétérocycle à cinq chaînons, p. ex. griséofulvine
• C12P 7/24 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carbonyle
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12R 1/19 - Escherichia coli

Abrégé

The present invention discloses a protein transformation method based on an amino acid knowledge graph and active learning, including: building an amino acid knowledge graph based on biochemical attributes of amino acids; enhancing protein data in combination with the amino acid knowledge graph to obtain enhanced protein data, and performing representation learning to obtain first enhanced protein representations; performing representation learning on the protein data or the protein data and the amino acid knowledge graph by using a pre-trained protein model to obtain second enhanced protein representations; synthesizing the first enhanced protein representations and the second enhanced protein representations to obtain enhanced protein representations; taking the enhanced protein representations as samples, and through active learning, screening out representative samples from the samples, manually annotating protein properties, and training a protein property prediction model by using the manually annotated representative samples; and performing protein transformation by using the protein property prediction model. Therefore, rapid and accurate protein transformation can be implemented.

Classes IPC  ?

• G16B 15/00 - TIC spécialement adaptées à l’analyse de structures moléculaires bidimensionnelles ou tridimensionnelles, p. ex. relations structurelles ou fonctionnelles ou alignement de structures
• G16B 35/00 - TIC spécialement adaptées aux bibliothèques combinatoires in silico d’acides nucléiques, de protéines ou de peptides
• G16B 40/20 - Analyse de données supervisée

Abrégé

325+δ25+δ3325+δ325+δ25+δδ perovskite, and improves the oxygen transmission performance of the material at the operating temperature of batteries.

Classes IPC  ?

• C04B 35/01 - Produits céramiques mis en forme, caractérisés par leur compositionCompositions céramiquesTraitement de poudres de composés inorganiques préalablement à la fabrication de produits céramiques à base d'oxydes
• H01M 4/90 - Emploi de matériau catalytique spécifié

Abrégé

A method and a device for remote acquisition of correlated pseudo-random numbers based on semi-trusted hardware. When applied to semi-trusted hardware, the method comprises: acquiring a random seed of a sender and a selected number of a receiver; generating a plurality of first correlated pseudo-random numbers and first commitment seeds according to the random seed and a predetermined category of the correlated pseudo-random number; generating a first commitment value and a first open value by a commitment mechanism according to the first correlated pseudo-random numbers and the first commitment seeds; generating a Merkle proof according to the first commitment value and the selected number; sending the first correlated pseudo-random numbers, the first commitment value, the first open value and the Merkle proof corresponding to the selected number to the receiver.

Classes IPC  ?

• G06F 7/58 - Générateurs de nombres aléatoires ou pseudo-aléatoires
• H04L 9/06 - Dispositions pour les communications secrètes ou protégéesProtocoles réseaux de sécurité l'appareil de chiffrement utilisant des registres à décalage ou des mémoires pour le codage par blocs, p. ex. système DES

Abrégé

The present disclosure relates to a two-plasmid system for prime editing in yeast, use thereof, and a method for gene prime editing in yeast. The two-plasmid system includes a first plasmid and a second plasmid. The first plasmid includes a sequence encoding for an epegRNA. The epegRNA is an RNA molecule including a motif at a 3′-terminus of a pegRNA, and the motif having a sequence as set forth in SEQ ID NO. 12. The second plasmid includes a sequence encoding for a fusion protein of a nucleic acid nickase nCas9 fused with a reverse transcriptase M-MLV RT. The two-plasmid expression system can be used in gene editing in yeast.

Classes IPC  ?

• C12N 15/81 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons pour levures
• C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
• C12N 9/22 - Ribonucléases
• C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
• C12N 15/62 - Séquences d'ADN codant pour des protéines de fusion
• C12N 15/90 - Introduction stable d'ADN étranger dans le chromosome

Abrégé

Provided is a method for processing lignocelluloses to produce D-glucaric acid, comprising: mixing various artificial microorganisms to give a microbial consortium, adapting the microbial consortium with an adaptation feedstock, and inoculating the adapted microbial consortium into a substrate obtained from the treatment of raw materials with subcritical water for fermentation. The microbial consortium comprises Trichoderma reesei and a yeast, and the adaptation feedstock is a filtrate obtained after the raw materials are pre-treated with subcritical water and filtered. The method features improved productivity and yield of D-glucaric acid, simplified process, and improved cost-efficiency.

Classes IPC  ?

• C12P 7/58 - Acides aldoniques, céto-aldoniques ou sacchariques
• C12R 1/885 - Trichoderma
• C12R 1/865 - Saccharomyces cerevisiae

Abrégé

Provided are a double-plasmid system suitable for yeast prime editing, use, and a gene editing method suitable for yeast prime editing. The double-plasmid system comprises a first plasmid and a second plasmid. The first plasmid comprises a sequence for encoding an epegRNA, which is an RNA molecule where a motif set forth in SEQ ID NO. 12 is introduced at the 3' terminus of a pegRNA. The second plasmid comprises a sequence for encoding a fusion protein formed by means of the fusion of nucleic acid nickase nCas9 and reverse transcriptase M-MLV RT. The double-plasmid expression system can be applied to yeast gene editing.

Classes IPC  ?

• C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
• C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
• C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
• C12N 15/90 - Introduction stable d'ADN étranger dans le chromosome
• C12N 9/22 - Ribonucléases

Abrégé

Disclosed in the present invention is a pre-trained deep neural network based superconducting material prediction method, comprising: first, fully using a large amount of unlabeled data, and pre-training same by means of an atomic-masked self-supervised learning mode to obtain a second embedded layer, a second fused layer and a second attention layer; then constructing a superconducting material prediction model by means of the above structure; and performing training by means of a loss function constructed by a small amount of real superconducting material labels and predicted superconducting material results, so as to obtain a superconducting material prediction model having relatively good robustness and capable of accurately determining whether a crystal is a superconducting material.

Classes IPC  ?

• G06F 30/27 - Optimisation, vérification ou simulation de l’objet conçu utilisant l’apprentissage automatique, p. ex. l’intelligence artificielle, les réseaux neuronaux, les machines à support de vecteur [MSV] ou l’apprentissage d’un modèle

Abrégé

Disclosed is a low-power and compact neuron circuit implementing a ReLU activation function including a first-layer synaptic array, a neuron transistor, a resistor, and a second-layer synaptic array. The neuron transistor is a MOS transistor having a threshold voltage-adjustable property, a gate electrode of the neuron transistor is connected to each voltage output end of the first-layer synaptic array, and a drain electrode of the neuron transistor is connected to each voltage input end of the second-layer synaptic array. Thus, it is possible to satisfy the decision computation and output of different synaptic array output values by adjusting the magnitude of the threshold voltage of the transistor. The neuron circuit requires only one transistor in cooperative connection with the first-layer synaptic array and the second-layer synaptic array to implement the ReLU activation function; therefore, a significant improvement is achieved in terms of energy efficiency, delay reduction, and space utilization.

Classes IPC  ?

• G06N 3/063 - Réalisation physique, c.-à-d. mise en œuvre matérielle de réseaux neuronaux, de neurones ou de parties de neurone utilisant des moyens électroniques
• G06N 3/048 - Fonctions d’activation

Abrégé

The present invention discloses a layer-number-controllable graphene derived from natural biomass and a preparation method thereof. The preparation method includes pulverizing 1-100 g of biomass to obtain a 50- to 300-mesh biomass scrap, and drying the biomass scrap at 60-100° C. to obtain a biomass precursor; mixing the biomass precursor with a Bronsted acid solution in a solid-liquid ratio of 0.1:10 to 2:100 g/mL, conducting sealing after discharging oxygen and introducing nitrogen, and then conducting heating for a reaction at 75-95° C. for 1-6 hours to obtain a graphene suspension; and conducting post-treatment on the graphene suspension to obtain a stable graphene dispersion, and then drying the stable graphene dispersion to obtain a graphene powder, where the post-treatment includes one or more of filtration washing, dialysis or ultrasonic treatment. According to the preparation method, the layer-number-controllable graphene is prepared by a mild chemical strategy at relatively low temperature with the biomass having high selectivity as a carbon source. The present invention further provides a layer-number-controllable graphene prepared by the method.

Classes IPC  ?

• C01B 32/184 - Préparation
• C01B 32/196 - Purification

Abrégé

Provided are a genetically engineered bacterium for producing catharanthine by fermentation, and use thereof. The catharanthine is produced by fermenting microorganisms with a simple carbon source as a substrate, thereby realizing the denovo synthesis of the catharanthine, enhancing the central metabolism of pichia pastoris, and providing a reference for the biosynthesis of terpene indole alkaloid. The engineered saccharomyces cerevisiae directly synthesizes the catharanthine by metabolizing methanol, and the yield of the catharanthine can reach 2.57 mg/L.

Classes IPC  ?

• C12N 1/19 - LevuresLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 15/53 - Oxydoréductases (1)
• C12N 1/38 - Stimulation chimique de la croissance ou de l'activité par addition de composés chimiques qui ne sont pas des facteurs essentiels de croissanceStimulation de la croissance par élimination d'un composé chimique
• C12N 15/81 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons pour levures
• C12P 17/18 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle contenant plusieurs hétérocycles condensés entre eux ou condensés avec un système carbocyclique commun, p. ex. rifamycine
• C12R 1/84 - Pichia

Abrégé

The present disclosure relates to the technical field of semiconductor integrated circuits and discloses a multi-mode array structure for in-memory computing, and a chip, including: an array of memory cells, function lines corresponding to all the memory cells measured by rows in the array of memory cells, and complementary function lines and bit lines BL corresponding to all the memory cells measured by columns in the array of memory cells. According to the present disclosure, the TCAM function and CNN and SNN operations are enabled; the multi-mode array for in-memory computing herein goes beyond the limits of the von Neumann architecture by integrating the multiple modes of storage and computation, achieving efficient operation and computation; in addition to solving the computing power problem, a new array mode is provided to promote the development of high-integration circuits.

Classes IPC  ?

• G06N 3/063 - Réalisation physique, c.-à-d. mise en œuvre matérielle de réseaux neuronaux, de neurones ou de parties de neurone utilisant des moyens électroniques
• G06N 3/0464 - Réseaux convolutifs [CNN, ConvNet]
• G06N 3/049 - Réseaux neuronaux temporels, p. ex. éléments à retard, neurones oscillants ou entrées impulsionnelles
• G11C 15/04 - Mémoires numériques dans lesquelles l'information, comportant une ou plusieurs parties caractéristiques, est écrite dans la mémoire et dans lesquelles l'information est lue au moyen de la recherche de l'une ou plusieurs de ces parties caractéristiques, c.-à-d. mémoires associatives ou mémoires adressables par leur contenu utilisant des éléments semi-conducteurs

Abrégé

Disclosed are a meso-2,3-butanediol dehydrogenase and a mutant and use thereof. The meso-2,3-butanediol dehydrogenase has an amino acid sequence set forth in SEQ ID NO: 1. According to the present invention, a meso-2,3-butanediol dehydrogenase with high-temperature resistance is found; on the basis of the meso-2,3-butanediol dehydrogenase, by using an accelerated sampling molecular dynamics simulation method to dynamically describe the release process of a product, the problem that high stability and high activity cannot be balanced is solved, and a mutant capable of catalyzing the preparation of meso-2,3-butanediol is obtained, which has very good high-temperature resistance. After heat treatment at 100 °C for 30 min, the residual enzyme activity is 23.9%. In catalyzing the formation of meso-2,3-butanediol by acetoin, the activity is increased by a factor of about 2-5. The product meso-2,3-butanediol obtained for the reaction has extremely high optical purity, which provides a broad application prospect for the production of meso-2,3-butanediol by bioconversion.

Classes IPC  ?

• C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
• C12N 15/53 - Oxydoréductases (1)
• C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12P 7/18 - Polyols
• C12R 1/19 - Escherichia coli

Abrégé

Disclosed are a protein conformation-aware representation learning method based on a pre-trained language model. The method comprises: acquiring a protein, which is formed by amino acid sequences, constructing different data sets according to protein conformations, and defining a prompt for each type of protein conformation; on the basis of a pre-trained language model, constructing a representation learning module, which is used for fusing an embedding of each type of prompt with an embedding of the protein, so as to obtain an embedding of the protein that is identified by the prompt; constructing a task module, which is used for performing task prediction on a task, which corresponds to each type of protein conformation, on the basis of the embedding of the protein that is identified by the prompt; constructing a loss function of each type of task on the basis of a task prediction result and a label, and updating model parameters of the representation learning module and the task module in view of loss functions of all types of tasks and the different data sets; and after the update of the model parameters ends, extracting the representation learning module as a protein representation module.

Classes IPC  ?

• G16B 5/00 - TIC spécialement adaptées à la modélisation ou aux simulations dans la biologie des systèmes, p. ex. réseaux de régulation génétique, réseaux d’interaction entre protéines ou réseaux métaboliques

Abrégé

Disclosed in the present invention is a protein modification method based on an amino acid knowledge graph and active learning. The method comprises: on the basis of biochemical attributes of amino acids, constructing an amino acid knowledge graph; in combination with the amino acid knowledge graph, performing data augmentation on protein data to obtain augmented protein data, and performing representation learning to obtain a first augmented protein representation; by using a pre-trained protein model, performing representation learning on the protein data, or on the protein data and the amino acid knowledge graph to obtain a second augmented protein representation; combining the first augmented protein representation and the second augmented protein representation to obtain an augmented protein representation; taking the augmented protein representation as samples, screening representative samples from the samples by using active learning, carrying out manual labeling of protein properties, and training a protein property prediction model by using manually labeled representative samples; and, by using the protein property prediction model, performing protein modification, so that rapid and accurate protein modification can be realized.

Classes IPC  ?

• G16B 5/00 - TIC spécialement adaptées à la modélisation ou aux simulations dans la biologie des systèmes, p. ex. réseaux de régulation génétique, réseaux d’interaction entre protéines ou réseaux métaboliques
• G06F 16/36 - Création d’outils sémantiques, p. ex. ontologie ou thésaurus

Abrégé

Provided is an application of an E41 gene and an E41 gene coding protein in regulation and control of plant embryonic development. A nucleotide sequence of the E41 gene is shown as any one of SEQ ID NOs:1-5, and an amino acid sequence of the E41 gene coding protein is shown as any one of SEQ ID NOs: 6-9. A plant peroxisomal protein encoded by the E41 gene can influence embryonic development, and the mutation of the E41 gene causes the lethal character of a homozygous mutant.

Classes IPC  ?

• C12N 15/29 - Gènes codant pour des protéines végétales, p. ex. thaumatine
• C07K 14/415 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de végétaux
• C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
• A01H 5/10 - Graines
• A01H 5/08 - Fruits
• A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
• A01H 6/82 - Solanaceae, p. ex. poivron, tabac, pomme de terre, tomate ou aubergine
• A01H 6/20 - Brassicaceae, p. ex. colza, brocoli ou roquette

Abrégé

Provided is a synthesis method for Euphorbia diterpenoid Pepluanol A. By using a cycloheptenone derivative as a reaction raw material, a key tricyclic skeleton is constructed by means of key steps such as a Negishi coupling reaction, a Diels-Alder reaction and a titanium free radical-catalyzed tandem cyclization reaction; and finally, a target product Euphorbia diterpenoid Pepluanol A is prepared by means of functional group conversion. The synthesis method has simple and convenient operations, can synthesize the product in 17 steps, has mild conditions, and can reach a total yield of 2.5% by controlling reaction conditions. The synthesis route is novel in design thought, raw materials are cheap and accessible, and the compatibility of all important functional groups is high; the synthesis of various derivatives of a Euphorbia diterpenoid structure containing the same 5/6/7-fused ring skeletons is facilitated, thus laying a foundation for the research on the structure-efficiency relationship of the compounds.

Classes IPC  ?

• C07C 45/42 - Préparation de composés comportant des groupes C=O liés uniquement à des atomes de carbone ou d'hydrogènePréparation des chélates de ces composés par hydrolyse
• C07C 49/727 - Composés non saturés comportant un groupe cétone faisant partie d'un cycle contenant des groupes hydroxyle polycycliques un groupe cétone faisant partie d'un système cyclique condensé
• C07F 7/18 - Composés comportant une ou plusieurs liaisons C—Si ainsi qu'une ou plusieurs liaisons C—O—Si
• C07C 45/29 - Préparation de composés comportant des groupes C=O liés uniquement à des atomes de carbone ou d'hydrogènePréparation des chélates de ces composés par oxydation de groupes hydroxyle
• C07C 45/64 - Préparation de composés comportant des groupes C=O liés uniquement à des atomes de carbone ou d'hydrogènePréparation des chélates de ces composés par des réactions ne créant pas de groupe C=O par introduction de groupes fonctionnels contenant de l'oxygène lié uniquement par liaison simple
• C07C 49/737 - Composés non saturés comportant un groupe cétone faisant partie d'un cycle contenant des groupes hydroxyle polycycliques un groupe cétone faisant partie d'un système cyclique condensé comportant trois cycles
• C07D 307/00 - Composés hétérocycliques contenant des cycles à cinq chaînons comportant un atome d'oxygène comme unique hétéro-atome du cycle
• C07D 493/08 - Systèmes pontés
• C07D 303/48 - Composés contenant des cycles oxirane avec des hétéro-atomes ou avec des atomes de carbone comportant trois liaisons à des hétéro-atomes avec au plus une liaison à un halogène, lié directement aux atomes de carbone du cycle, p. ex. radicaux ester ou nitrile
• C07C 303/26 - Préparation d'esters ou d'amides d'acides sulfuriquesPréparation d'acides sulfoniques ou de leurs esters, halogénures, anhydrides ou amides d'esters d'acides sulfoniques
• C07C 309/66 - Méthanesulfonates
• C07C 45/63 - Préparation de composés comportant des groupes C=O liés uniquement à des atomes de carbone ou d'hydrogènePréparation des chélates de ces composés par des réactions ne créant pas de groupe C=O par introduction d'atomes d'halogènePréparation de composés comportant des groupes C=O liés uniquement à des atomes de carbone ou d'hydrogènePréparation des chélates de ces composés par des réactions ne créant pas de groupe C=O par substitution d'atomes d'halogène par des atomes d'autres halogènes
• C07C 45/68 - Préparation de composés comportant des groupes C=O liés uniquement à des atomes de carbone ou d'hydrogènePréparation des chélates de ces composés par des réactions ne créant pas de groupe C=O par isomérisationPréparation de composés comportant des groupes C=O liés uniquement à des atomes de carbone ou d'hydrogènePréparation des chélates de ces composés par des réactions ne créant pas de groupe C=O par modification de la taille du squelette carboné par augmentation du nombre d'atomes de carbone
• C07C 45/69 - Préparation de composés comportant des groupes C=O liés uniquement à des atomes de carbone ou d'hydrogènePréparation des chélates de ces composés par des réactions ne créant pas de groupe C=O par isomérisationPréparation de composés comportant des groupes C=O liés uniquement à des atomes de carbone ou d'hydrogènePréparation des chélates de ces composés par des réactions ne créant pas de groupe C=O par modification de la taille du squelette carboné par augmentation du nombre d'atomes de carbone par addition à des liaisons doubles ou triples carbone-carbone
• C07C 49/753 - Composés non saturés comportant un groupe cétone faisant partie d'un cycle contenant des groupes éther, des groupes , des groupes ou des groupes

Abrégé

36nn, where n is an integer larger than 1, and L represents HCOO-; the metal ion M is at least one of Co2+, Ni2+and Mg2+; and the carboxylic acid organic ligand is HCOOH.

Classes IPC  ?

• C07C 15/073 - Éthylbenzène
• C07C 7/12 - Purification, séparation ou stabilisation d'hydrocarburesEmploi d'additifs par adsorption, c.-à-d. purification ou séparation d'hydrocarbures à l'aide de solides, p. ex. à l'aide d'échangeurs d'ions
• B01D 53/04 - Séparation de gaz ou de vapeursRécupération de vapeurs de solvants volatils dans les gazÉpuration chimique ou biologique des gaz résiduaires, p. ex. gaz d'échappement des moteurs à combustion, fumées, vapeurs, gaz de combustion ou aérosols par adsorption, p. ex. chromatographie préparatoire en phase gazeuse avec adsorbants fixes
• B01D 53/047 - Adsorption à pression alternée
• B01J 20/22 - Compositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation contenant une substance organique

Abrégé

Disclosed are a yeast engineering bacterium for fermentative production of α-santalene and use thereof. Yeast is used as an original strain, and by means of the introduction of a gene construct, a yeast engineering bacterium that can efficiently produce α-santalene is obtained, with a fermentation yield of nearly 3.0 g/L in a shake flask and a fermentation yield of up to 15 g/L in a 1 L fermentor. By means of a high expression of a key rate-limiting enzyme in a metabolic pathway, an enhanced precursor supply, and a multi-copy strategy, metabolic fluxes in a biosynthetic pathway of α-sandalene are improved. Pichia pastoris is used in the efficient production of α-santalene, and the production is characterized by short period, environmental friendliness, and resource conservation.

Classes IPC  ?

• C12N 1/19 - LevuresLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
• C12N 15/53 - Oxydoréductases (1)
• C12N 15/54 - Transférases (2)
• C12N 15/60 - Lyases (4)
• C12N 15/61 - Isomérases (5)
• C12N 15/81 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons pour levures
• C12P 15/00 - Préparation de composés contenant au moins trois carbocycles condensés
• C12R 1/84 - Pichia
• C12R 1/85 - Saccharomyces

Abrégé

Provided are an accurate PAM-limitation-free adenine base editor and a use thereof. The adenine base editor comprises a coding gene of an adenine deaminase mutant TadA8eF148A having a nucleotide sequence as shown in SEQ ID NO. 2 and a coding gene of a SpRY nuclease mutant having a nucleotide sequence as shown in SEQ ID NO. 3. The adenine base editor can be used for gene editing reagent preparation or gene editing for non-disease-treatment purposes, is free of PAM limitation in editing range, has high editing accuracy and efficiency, and can be used for G>A single base repair.

Classes IPC  ?

• C12N 15/62 - Séquences d'ADN codant pour des protéines de fusion
• C12N 9/78 - Hydrolases (3.) agissant sur les liaisons carbone-azote autres que les liaisons peptidiques (3.5)
• C12N 9/22 - Ribonucléases
• C12N 15/55 - Hydrolases (3)
• C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
• C12N 15/85 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules animales

Abrégé

A privacy protection biometric authentication method and apparatus, and an electronic device. The method comprises: constructing a corresponding biometric data template according to a biometric information data set input when a user is registered (S11); generating a pair of public and private keys by using an asymmetric cryptography technique (S12); generating encrypted biometric data by using a secret sharing scheme and an OKVS technique and according to the biometric data template and the private key (S13); sending the public key and the encrypted biometric data to a server (S14); recovering the private key by using the OKVS technique and according to biometric data input during user verification and the encrypted biometric data (S15); constructing a signature according to the recovered private key and the corresponding public key (S16); and sending the signature to the server, so that the server verifies the user according to the public key and the signature (S17). The method solves the technical problems in the relevant art whereby the amount of stored data is large, and two systems in which biometric information of the same person is encrypted by using a fuzzy vault technique can be mutually unlocked.

Classes IPC  ?

• G06F 21/32 - Authentification de l’utilisateur par données biométriques, p. ex. empreintes digitales, balayages de l’iris ou empreintes vocales

Abrégé

Provided is a method for improving the efficiency of integrated bioprocessing by means of using artificial microorganisms, which relates to the technical field of microorganisms. The method comprises the following steps: mixing a plurality of artificial microorganisms to obtain a mixed micropopulation; and mixing the mixed flora with raw materials, and performing fermentation to complete the processing. The technical problems of low output, low production and low yield of integrated bioprocessing at present can be solved.

Classes IPC  ?

• C12P 39/00 - Procédés faisant intervenir simultanément des micro-organismes de différents genres dans le même procédé
• C12P 7/58 - Acides aldoniques, céto-aldoniques ou sacchariques
• C12R 1/865 - Saccharomyces cerevisiae
• C12R 1/885 - Trichoderma

Abrégé

The present disclosure discloses an amino acid helical array film and a preparation method thereof. The amino acid helical array film comprises a substrate and an amino acid helical array uniformly deposited on the substrate. Each amino acid helix is obtained by self-assembling an amino acid with a modifying group. The amino acid is selected from one or more of twenty common natural amino acids or adjacent isomers thereof. The modifying group comprises an N-terminal protecting group and a C-terminal protecting group, wherein the N-terminal protecting group is selected from one or more of carbobenzoxy, a lipid group, t-butoxycarbonyl, and 9-fluorenylmethoxycarbonyl, and the C-terminal protecting group is selected from one or more of nitrophenyl ester, a lipoxy group, and an acylamino group.

Classes IPC  ?

• C09D 177/04 - Polyamides dérivés d'acides alpha-aminocarboxyliques
• C23C 14/24 - Évaporation sous vide
• C23C 14/12 - Composé organique

Abrégé

A method and apparatus for remotely acquiring a correlated randomness on the basis of semi-trusted hardware. The method, when applied to semi-trusted hardware, comprises: acquiring a random seed of a sender and a choice number of a receiver (S11); generating several first correlated randomnesses and first commitment seeds on the basis of the random seed and a predetermined correlated randomness type (S12); on the basis of the several first correlated randomnesses and first commitment seeds, generating a first commitment value and a first open value using a commitment mechanism (S13); generating a Merkle proof on the basis of the first commitment value and the choice number (S14); and sending to the receiver the first correlated randomness corresponding to the choice number, and the first commitment value, the first open value and the Merkle proof, such that the receiver verifies the first commitment value on the basis of a Merkle root hash sent by the sender and the Merkle proof, and verifies, on the basis of the first commitment value that has passed verification and the first open value, the correlated randomness corresponding to the choice number (S15).

Classes IPC  ?

• H04L 9/32 - Dispositions pour les communications secrètes ou protégéesProtocoles réseaux de sécurité comprenant des moyens pour vérifier l'identité ou l'autorisation d'un utilisateur du système

Abrégé

A positioning platform based on an electromagnetic actuator, comprising an actuator stator assembly (30) on which a sliding guide groove (21) is formed; an actuator mover assembly (40), which is arranged in the actuator stator assembly (30) and can slide along the sliding guide groove (21) under an electromagnetic force; a moving platform assembly (50), which is driven by the actuator mover assembly (40) to slide along the sliding guide groove (21); and a deceleration driving assembly (60), having one end connected to the actuator mover assembly (40) and the other end connected to the moving platform assembly (50), wherein the actuator mover assembly (40) can selectively drive, by means of the deceleration driving assembly (60), the moving platform assembly (50) to move at different moving speeds.

Classes IPC  ?

• H02K 41/02 - Moteurs linéairesMoteurs sectionnels
• H02K 7/116 - Association structurelle avec des embrayages, des freins, des engrenages, des poulies ou des démarreurs mécaniques avec des engrenages

Abrégé

A two-stage multi-degree-of-freedom space position precision stabilization system (100). The two-stage multi-degree-of-freedom space position precision stabilization system (100) comprises: a motion platform (1); a secondary platform (2), which is connected to the motion platform (1) and is located below the motion platform (1), wherein the secondary platform (2) is provided with a second electromagnetic driving assembly (26) for adjusting the position of the motion platform (1); a primary platform (3), which is connected to the secondary platform (2) and is located below the secondary platform (2), wherein the primary platform (3) is provided with a first electromagnetic driving assembly (37) for adjusting the secondary platform (2), and thus adjusting the position of the motion platform (1); and a controller (4), which is used for controlling the first electromagnetic driving assembly (37) and the second electromagnetic driving assembly (26).

Classes IPC  ?

• H02K 41/02 - Moteurs linéairesMoteurs sectionnels
• H02K 11/22 - Dispositifs optiques

Abrégé

A positioning platform based on an electromagnetic actuator is provided. The positioning platform includes: an actuator stator component, an actuator mover component, a moving platform component, and a deceleration driving component. The actuator stator component is provided with a sliding guidance groove. The actuator mover component is disposed in the actuator stator component and capable of sliding by electromagnetic force along the sliding guidance groove. The actuator mover component is capable of driving the moving platform component to slide along the sliding guidance groove. An end of the deceleration driving component is connected to the actuator mover component, the other end is connected to the moving platform component. The actuator mover component is capable of selectively driving the moving platform component to move at different speeds by the deceleration driving component.

Classes IPC  ?

• H02K 41/03 - Moteurs synchronesMoteurs pas à pasMoteurs à réluctance

Abrégé

The present disclosure provides a silicon carbide trench gate metal oxide semiconductor field effect transistor (MOSFET) and a method for manufacturing thereof. The silicon carbide trench gate MOSFET includes: a substrate having a first doping type, an epitaxial layer formed on the substrate and having the first doping type, an epitaxial well region formed above the epitaxial layer and having a second doping type, a first source contact region formed in the epitaxial well region and having the first doping type, a second source contact region formed in the epitaxial well region and having the second doping type, a trench gate, a source electrode and a drain electrode, wherein the trench gate includes a gate dielectric and a gate electrode, the silicon carbide trench gate MOSFET further includes a injection-type current diffusion region, which is wrapped around the bottom of the trench gate and has the first doping type.

Classes IPC  ?

• H01L 29/06 - Corps semi-conducteurs caractérisés par les formes, les dimensions relatives, ou les dispositions des régions semi-conductrices
• H01L 29/16 - Corps semi-conducteurs caractérisés par les matériaux dont ils sont constitués comprenant, mis à part les matériaux de dopage ou autres impuretés, seulement des éléments du groupe IV de la classification périodique, sous forme non combinée
• H01L 29/10 - Corps semi-conducteurs caractérisés par les formes, les dimensions relatives, ou les dispositions des régions semi-conductrices avec des régions semi-conductrices connectées à une électrode ne transportant pas le courant à redresser, amplifier ou commuter, cette électrode faisant partie d'un dispositif à semi-conducteur qui comporte trois électrodes ou plus
• H01L 29/78 - Transistors à effet de champ l'effet de champ étant produit par une porte isolée
• H01L 21/04 - Fabrication ou traitement des dispositifs à semi-conducteurs ou de leurs parties constitutives les dispositifs ayant des barrières de potentiel, p. ex. une jonction PN, une région d'appauvrissement ou une région de concentration de porteurs de charges
• H01L 29/66 - Types de dispositifs semi-conducteurs

Abrégé

The present disclosure relates to the technical field of silicon carbide processing, and discloses a method and device for preferential etching of dislocation of a silicon carbide wafer. According to the method and device of the present disclosure, a concentration of the etchant is effectively reduced while the high-temperature etching activity is guaranteed, the dislocations on the carbon surface and the silicon surface of the silicon carbide wafer are exposed, and dislocation etching pits with high distinguishing degree are obtained on the carbon surface and the silicon surface of the silicon carbide wafer and thus identified clearly.

Classes IPC  ?

• H01L 21/02 - Fabrication ou traitement des dispositifs à semi-conducteurs ou de leurs parties constitutives

Abrégé

A trench-gate power MOSFET with optimized layout, comprising: a substrate; a first semiconductor region formed on the substrate, having a first doping type; mutually separated trench isolation gate structure, formed on the first semiconductor region, the trench isolation gate structure includes an gate oxide layer and a gate electrode; a second semiconductor region and a third semiconductor region formed between any two adjacent structures of mutually separated trench isolation gate structures; and a first shielding region, formed under each of the third semiconductor regions, connecting simultaneously with multiple mutually separated trench isolation structures.

Classes IPC  ?

• H01L 29/78 - Transistors à effet de champ l'effet de champ étant produit par une porte isolée
• H01L 29/06 - Corps semi-conducteurs caractérisés par les formes, les dimensions relatives, ou les dispositions des régions semi-conductrices
• H01L 29/423 - Electrodes caractérisées par leur forme, leurs dimensions relatives ou leur disposition relative ne transportant pas le courant à redresser, à amplifier ou à commuter

Abrégé

The present disclosure relates to the field of manufacturing of silicon carbide (SiC) single crystal wafers, and discloses a stripping method and a stripping device for SiC single crystal wafers. The single crystal wafers obtained by the present disclosure have no damage layer or stress residue on surfaces or sub-surfaces, and are simple in operation and low in cost.

Classes IPC  ?

• C25F 3/12 - Attaque de surface des matériaux semi-conducteurs
• C30B 33/10 - Gravure dans des solutions ou des bains fondus
• C30B 29/36 - Carbures

Abrégé

A trench gate silicon carbide MOSFET with high reliability, including: An N+ type substrate, an N− type drift region, a first P type region, a P+ type contact region, an N+ type contact region, an N type equivalent resistance region between the first P type region and the N+ type contact region, a gate dielectric layer, a trench gate, an isolation dielectric layer, a source electrode and a drain electrode.

Classes IPC  ?

• H01L 29/78 - Transistors à effet de champ l'effet de champ étant produit par une porte isolée
• H01L 29/16 - Corps semi-conducteurs caractérisés par les matériaux dont ils sont constitués comprenant, mis à part les matériaux de dopage ou autres impuretés, seulement des éléments du groupe IV de la classification périodique, sous forme non combinée
• H01L 29/06 - Corps semi-conducteurs caractérisés par les formes, les dimensions relatives, ou les dispositions des régions semi-conductrices
• H01L 29/10 - Corps semi-conducteurs caractérisés par les formes, les dimensions relatives, ou les dispositions des régions semi-conductrices avec des régions semi-conductrices connectées à une électrode ne transportant pas le courant à redresser, amplifier ou commuter, cette électrode faisant partie d'un dispositif à semi-conducteur qui comporte trois électrodes ou plus

Abrégé

A trench-gate MOSFET with electric field shielding region, has a substrate; a source electrode; a drain electrode; a semiconductor region with a first doping type formed on the substrate; a trench-gate, a plurality of electric field shielding regions with a second doping type formed under a surface of the semiconductor region, wherein the electric field shielding region intersects the trench-gate at an angle; a source electrode region formed on both sides of the trench-gate is divided into a plurality of source electrode sub-regions by the plurality of electric field shielding regions.

Classes IPC  ?

• H01L 29/78 - Transistors à effet de champ l'effet de champ étant produit par une porte isolée
• H01L 29/06 - Corps semi-conducteurs caractérisés par les formes, les dimensions relatives, ou les dispositions des régions semi-conductrices
• H01L 29/417 - Electrodes caractérisées par leur forme, leurs dimensions relatives ou leur disposition relative transportant le courant à redresser, à amplifier ou à commuter

Abrégé

The present disclosure provides a method to fabricate three-dimensional transparent glass utilizing polymer plasticity, including the following steps. In step 1, synthesize polymer-glass powder composite containing dynamic chemical bonds, the bond exchange catalyst is added during the synthesis process, and then cure to obtain a two-dimensional sheet shape I, the bond exchange catalyst is used to activate a dynamic chemical bond in step 2. In step 2, shape the two-dimensional sheet shape I obtained in step 1 into a complex three-dimensional shape II under the conditions of the effect of an external force and the activable dynamic chemical bond. In step 3, pyrolyze the composite precursor at high temperature to obtain transparent glass with complex three-dimensional shape II. The present disclosure provides a method in shaping the transparent glass with complex geometries by unique polymer plasticity in lower temperature.

Classes IPC  ?

• C03B 19/00 - Autres méthodes de façonnage du verre
• C03B 19/01 - Autres méthodes de façonnage du verre par fusion progressive d'une poudre de verre sur un substrat de formage, c.-à-d. accrétion
• C03B 20/00 - Procédés spécialement adaptés à la fabrication d'articles en quartz ou en silice fondue
• C03C 3/04 - Compositions pour la fabrication du verre contenant de la silice
• C03B 19/06 - Autres méthodes de façonnage du verre par frittage

Abrégé

The present invention provides a method to prepare polymer materials with interlocked porous structures by freezing and demulsification, which includes: (1) Preparing an emulsion containing uncrosslinked polymers and crosslinking agents. The uncrosslinked polymers are presented in the organic phase, and the crosslinking agents are presented in the organic phase or water phase. Under freezing, the demulsification is occurred which leads to the interaction between polymers and crosslinking agents, and the crosslinked materials are obtained. (2) After removing the ice crystals, polymer materials with interlocked porous structures are synthesized. The method provided by the present invention is simple to operate, and can well adjust the porous structures of obtained porous polymer materials. In addition, it is suitable for large scale manufacturing. At the same time, this process can form different functional porous polymer materials by simply changing the used monomers. Particularly, it can prepare melt-blown fabrics with antibacterial property, high-throughput vertical porous structures and high-temperature sterilizable feature, therefore, it can be used to manufacture medical products such as masks.

Classes IPC  ?

• C08J 9/28 - Mise en œuvre de substances macromoléculaires pour produire des matériaux ou objets poreux ou alvéolairesLeur post-traitement par élimination d'une phase liquide d'un objet ou d'une composition macromoléculaire, p. ex. par séchage du coagulum
• B01D 39/16 - Autres substances filtrantes autoportantes en substance organique, p. ex. fibres synthétiques
• C08F 20/14 - Esters méthyliques
• C08F 20/18 - Esters des alcools ou des phénols monohydriques des phénols ou des alcools contenant plusieurs atomes de carbone avec l'acide acrylique ou l'acide méthacrylique