Abrégé

The present disclosure relates to an exosome isolation method, more specifically, a method of isolating exosomes with high efficiency and high purity, including deproteinization, exosome aggregation, exosome binding, and exosome isolation. The method of isolating exosomes according to the present disclosure may be applied to all samples, such as body fluids and cell culture fluids, and thus, is characterized as a technique applicable to samples universally, and since total time required for exosome isolation is within 40 minutes, the method is time efficient, and since the method is capable of isolating 25 times more exosomes or greater than ultracentrifugation, the method may isolate exosomes with high efficiency and high purity. Therefore, the isolation method of the present disclosure and the exosomes isolated by the method are expected to be widely applicable in research on diagnosis or treatment methods requiring high-purity exosomes.

Classes IPC  ?

• C12N 5/0775 - Cellules souches mésenchymateusesCellules souches dérivées du tissu adipeux
• B01D 21/26 - Séparation du sédiment avec emploi de la force centrifuge
• B01D 71/10 - CelluloseCellulose modifiée
• B01D 71/14 - Esters d'acides organiques
• B01D 71/34 - Fluorure de polyvinylidène
• C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains

Abrégé

Provided are a method of preparing atelocollagen and use thereof, more specifically, a method of removing immunogens from pig skin-derived collagen and preparing high-purity atelocollagen with high yield, and use thereof. Atelocollagen prepared with high yield, according to a preparation method of the present disclosure, has high purity, a low immune response, no cytotoxicity, excellent in vivo biodegradability, and high safety, and therefore, may be used in a form of a bio-ink capable of forming high-resolution structures with excellent morphological characteristics and division ability, and therefore, is expected to be used for the development of a product for tissue and regeneration engineering, including a cell scaffold capable of easily forming blood vessels and controlling drug delivery.

Classes IPC  ?

• C07K 1/12 - Procédés généraux de préparation de peptides par hydrolyse
• C07K 14/78 - Peptides du tissu connectif, p. ex. collagène, élastine, laminine, fibronectine, vitronectine ou globuline insoluble à froid [CIG]
• C07K 1/36 - ExtractionSéparationPurification par une combinaison de plusieurs procédés de types différents
• C09D 11/04 - Encres d’imprimerie à base de protéines
• A61L 27/24 - Collagène

Abrégé

The present invention relates to a composition which is for promoting angiogenesis and comprises thymosin β-4 fusion proteins. The thymosin β-4 fusion proteins according to an aspect of the present invention can very effectively pass through cell membranes to induce angiogenesis as well as inhibit apoptosis, and can induce wound healing, and thus can be effectively used as a therapeutic agent for the regeneration of blood vessels or other damaged tissues or the treatment of ischemic diseases or diabetic foot ulcers.

Classes IPC  ?

• A61K 38/00 - Préparations médicinales contenant des peptides
• A61K 38/22 - Hormones
• A61K 47/64 - Conjugués médicament-peptide, médicament-protéine ou médicament-acide polyaminé, c.-à-d. l’agent de modification étant un peptide, une protéine ou un acide polyaminé lié par covalence ou complexé à un agent thérapeutiquement actif
• A61P 17/00 - Médicaments pour le traitement des troubles dermatologiques
• A61P 41/00 - Médicaments utilisés en chirurgie, p. ex. adjuvants chirurgicaux pour la prévention des adhérences ou pour le remplacement de l'humeur vitrée
• A61P 43/00 - Médicaments pour des utilisations spécifiques, non prévus dans les groupes

Abrégé

The present invention relates to an atelocollagen-based bioink composition, and a method for manufacturing a three-dimensional structure by using same. The bioink composition according to one aspect has excellent cell viability and cell adhesion/proliferation and biodegradability, and has excellent printability, and thus can be effectively used for manufacturing a tissue-like structure by using a 3D bioprinter.

Classes IPC  ?

• C09D 11/04 - Encres d’imprimerie à base de protéines
• A61L 27/52 - Hydrogels ou hydrocolloïdes
• A61L 27/38 - Cellules animales

Abrégé

The present invention relates to an exosome isolation method and, more particularly, to a method for isolating exosomes with high efficiency and high purity, comprising: a deproteinization step; an exosome aggregation step; an exosome binding step; and an exosome isolation step. The exosome isolation method according to the present invention is a versatile technique for samples since the method can be applied to all samples such as body fluids and cell cultures, has a total exosome isolation time of within 40 minutes, which is effective in saving time, and can isolate 25 times or more exosomes as compared to ultracentrifugation, thus enabling the isolation of exosomes with high efficiency and high purity. Thus, the isolation method of the present invention and exosomes isolated by the method are expected to be widely used in research on diagnosis or treatment methods that require high-purity exosomes.

Classes IPC  ?

• C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet

Abrégé

The present invention relates to a method for preparing atelocollagen and a use thereof and, more particularly, to a method for removing an immunogen from pig skin-derived collagen and preparing atelocollagen in high purity and high yield, and a use thereof. Atelocollagen prepared in high yield according to the preparation method of the present invention has high purity, low immune response, no cytotoxicity, excellent in vivo biodegradability, high safety, and excellent morphological characteristics and cleavage ability, and thus can also be used in the form of a bio-ink capable of forming a high-resolution structure. Therefore, the atelocollagen is expected to be used as a product for tissue and regenerative engineering, including the development of a cell scaffold that can easily form blood vessels that can control drug delivery.

Classes IPC  ?

• C07K 1/36 - ExtractionSéparationPurification par une combinaison de plusieurs procédés de types différents
• C07K 1/12 - Procédés généraux de préparation de peptides par hydrolyse
• C07K 1/30 - ExtractionSéparationPurification par précipitation
• C07K 1/14 - ExtractionSéparationPurification
• C07K 14/78 - Peptides du tissu connectif, p. ex. collagène, élastine, laminine, fibronectine, vitronectine ou globuline insoluble à froid [CIG]
• C09D 11/04 - Encres d’imprimerie à base de protéines
• A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
• A61L 27/56 - Matériaux poreux ou cellulaires