Abrégé

Disclosed in the present invention is a method for obtaining tetraploid Blumea balsamifera (L.) DC. adventitious buds, comprising: selecting a root cutting section of diploid Blumea balsamifera (L.) DC. as an explant; culturing by using a doubling culture medium containing 0.025-0.1 mg/L of NAA, 1.0-2.0 mg/L of 6-BA, and 90-150mg/L of colchicine; and inducing explant cells for chromosome doubling and adventitious bud differentiation simultaneously. According to the present invention, the blank of the Blumea balsamifera (L.) DC. root as an explant is filled, and the effective explant source of the Blumea balsamifera (L.) DC. in the propagation multiplication and biotechnological breeding process is increased. More importantly, according to the present invention, the root cells of Blumea balsamifera (L.) DC. are directly differentiated at the same time of chromosome doubling to form adventitious buds, and the process of forming the callus is not required, such that the technical link is simplified, and the variation of regeneration buds and the generation of chimera are reduced. The present invention is applied to polyploid breeding of the Blumea balsamifera (L.) DC., time and labor cost can be remarkably saved, and the working efficiency is improved.

Classes IPC  ?

• A01H 1/08 - Méthodes ou appareils pour modifier le nombre des chromosomes
• A01H 4/00 - Reproduction de plantes par des techniques de culture de tissus

Abrégé

The present invention provides a culture method for generating an adventitious bud by inducing blumea balsamifera root cell differentiation in one step. A root segment of blumea balsamifera is selected as an explant for culture, an adventitious bud induction culture medium is designed, and the explant can be induced to directly differentiate into an adventitious bud by using the culture medium. The present invention fills the blank of a blumea balsamifera root serving as the explant, and increases the effective explant source of blumea balsamifera in a propagation and proliferation process. In addition, according to the present invention, the root cell of blumea balsamifera is directly differentiated into the adventitious bud, and there is no process of forming a callus, and thus, the technical link is simplified, the variation of a regenerated bud and the generation of chimera are reduced, the accuracy of the directional mutagenesis or genetic transformation of blumea balsamifera is improved, and the present invention can be applied to blumea balsamifera breeding, and can remarkably reduce the time and the labor cost, and improve the working efficiency.

Classes IPC  ?

• A01H 4/00 - Reproduction de plantes par des techniques de culture de tissus

Abrégé

B. balsamifera are directly differentiated into adventitious buds while chromosomes are doubled, and a callus formation process is not needed, so that the technical links are simplified and the variation of regeneration buds and the generation of chimeras are reduced.

Classes IPC  ?

• A01H 1/08 - Méthodes ou appareils pour modifier le nombre des chromosomes
• A01H 4/00 - Reproduction de plantes par des techniques de culture de tissus

Abrégé

An herbal tea containing fresh rhizoma phragmitis and Lonicera caerulea vinegar, and a preparation method therefor. The herbal tea comprises the following raw materials in parts by weight: 9-200 parts fresh citron, 2-50 parts fresh rhizoma phragmitis, 2-50 parts Lonicera caerulea fruit, 2-50 parts mulberry leaf, 2-40 parts black sesame, 1-25 parts chenpi, 1-20 parts Stevia rebaudiana, 0.5-10 parts spiny jujube kernel, 0.5-10 parts mint, 8-160 parts crystal sugar, 4-85 parts maltose, 0.3-10 parts salt, 500-10000 parts purified water, 1-25 parts 50º-65º pure grain alcohol, 1-20 parts brown sugar, 0.01-0.15 parts dry yeast, and 10-250 parts cooled boiled water. Using microbial fermentation and salt pickling methods, the raw materials are synergistically applied so as to promote metabolism, effectively regulate the gastrointestinal tracts, regulate acid-base balance, eliminate fatigue, enhance the effects of promoting qi circulation to relieve pain, and promote stomach digestion.

Classes IPC  ?

• A23F 3/34 - Succédanés du thé, p. ex. matéExtraits ou infusions de ceux-ci

Abrégé

Provided are a compound cold process soap containing L-borneol, and a preparation method therefor. The compound cold process soap comprises a compound medicine and a base oil; the compound medicine comprises the following raw materials in parts by weight: 0.1-0.3 part of L-borneol, 3-7 parts of Blumea balsamifera pure dew, 0.1-0.2 part of Callicarpa nudiflora extract powder, 0.1-0.5 part of camellia seed oil, and 1-3 parts of seven white powder; the base oil comprises the following raw materials in percentage by weight: 20%-30% of coconut oil, 30%-50% of olive oil, 10%-30% of shea butter, 5%-15% of evening primrose oil, and 3%-7% of macadamia nut oil; alkaline water is added into the compound medicine and the base oil, and saponification is carried out in a low-temperature environment. The prepared cold process soap has high antibacterial performance and oxidation resistance.

Classes IPC  ?

• C11D 9/02 - Savons aux alcalis ou à l'ammoniaque
• C11D 9/38 - Produits de composition non exactement définie
• C11D 9/26 - Composés organiques contenant de l'oxygène
• C11D 9/60 - Mélanges de composés
• C11D 3/48 - Agents médicinaux ou de désinfection
• C11D 3/00 - Autres composés entrant dans les compositions détergentes couvertes par le groupe

Abrégé

millettia root, comprising the following steps: S1, material treatment, S2, cold soaking, S3 enzymatic hydrolysis, S4 extract extraction, and S5 ultrasonic-microwave synergistic extraction. The extraction method of the present disclosure extracts relatively high content of total saponins, and has relatively high yield of saponins and low content of impurities, and each step acts synergistically to solve the problems of relatively low total saponin content, more impumayrities and bubbling in the extraction process.

Classes IPC  ?

• C07H 1/08 - SéparationPurification à partir de produits naturels
• B01D 11/02 - Extraction par solvants de solides
• B01D 11/04 - Extraction par solvants de solutions

Abrégé

The present invention provides binaphtho r-pyrone compounds and an application thereof against plant anthracnose. Compounds having structures represented by formulas (1)-(4) or pharmaceutically acceptable salts thereof provided in the present invention have activity inhibiting plant anthracnose, and can be used as active ingredients for preventing and controlling plant anthracnose diseases. Exemplary embodiments prove that the compounds provided in the present invention have a strong inhibitory effect on various plant anthracnose bacteria. The effective amount of the dinaphtho r-pyrone compounds of the present invention is 4-32 μg/mL, and preferably 4-16 μg/mL.

Classes IPC  ?

• C07D 311/92 - NaphtopyrannesNaphtopyrannes hydrogénés
• A01N 43/16 - Biocides, produits repoussant ou attirant les animaux nuisibles, ou régulateurs de croissance des végétaux, contenant des composés hétérocycliques comportant des cycles avec un ou plusieurs atomes d'oxygène ou de soufre comme uniques hétéro-atomes du cycle avec un hétéro-atome des cycles à six chaînons avec l'oxygène comme hétéro-atome du cycle
• A01P 3/00 - Fongicides
• C12P 17/06 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle contenant un hétérocycle à six chaînons, p. ex. fluorescéine

Abrégé

Provided is a method for extracting a flavonoid component in millettia speciosa champ, comprising the following steps: performing quick-freezing treatment on millettia speciosa champ; preparing a eutectic solvent-ethanol aqueous two-phase extraction solvent; performing ultrasonic assisted treatment, concentration, filtration and washing to obtain a flavonoid component; and preparing a aqueous two-phase extraction solvent. Two ultrasonic assistance extractions further improves the purity and extraction rate of flavone, and relatively high antioxidant activity is achieved.

Classes IPC  ?

• A61K 36/486 - Millettia
• A61P 39/06 - Antiradicaux libres ou antioxydants
• A61K 125/00 - Parties de plantes contenant des racines, des bulbes, des tubercules ou des rhizomes, ou obtenues à partir de ces éléments

Abrégé

The present invention provides an azaphilone polymer compound, and a preparation method therefor and the use thereof. According to the present invention, muyocopronlaterale is fermented and cultured, and a fermentation product is separated to obtain a high-purity compound monomer. The compound has a novel structural skeleton, and the structure of the compound is represented by formula (I). Experiments show that the compound has a strong effect of inhibiting plant root elongation, and can be used as a new-generation herbicide active ingredient.

Classes IPC  ?

• C07D 311/76 - Benzo [c] pyrannes
• C12P 17/06 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle contenant un hétérocycle à six chaînons, p. ex. fluorescéine
• C12N 1/14 - ChampignonsLeurs milieux de culture
• A01N 43/16 - Biocides, produits repoussant ou attirant les animaux nuisibles, ou régulateurs de croissance des végétaux, contenant des composés hétérocycliques comportant des cycles avec un ou plusieurs atomes d'oxygène ou de soufre comme uniques hétéro-atomes du cycle avec un hétéro-atome des cycles à six chaînons avec l'oxygène comme hétéro-atome du cycle
• A01P 13/00 - HerbicidesAlgicides
• C12R 1/645 - Champignons

Abrégé

A method for extracting Tsaoko fructus essential oil for inhibiting candida tropicalis and the Tsaoko fructus essential oil. The extraction method comprises the following steps: weighing the ripe fruit of Tsaoko fructus, crushing and sieving same with a 30-50 mesh sieve to obtain a powder; mixing the powder with water, leaving the mixture to stand for 2-5 h, and then distilling the mixture with steam at 180°C-260°C for 5-8 h. Efficient extraction of the Tsaoko fructus essential oil is achieved by means of a steam distillation method, and the extraction rate reaches not less than 1.7%. The obtained Tsaoko fructus essential oil shows extremely strong inhibitory activity on candida tropicalis. The diameter of the bacteriostatic circle is not less than 15 mm, the MIC reaches not more than 0.195 μL/mL, and the MBC reaches not more than 0.781 μL/mL.

Classes IPC  ?

• C11B 9/02 - Obtention ou raffinage des huiles essentielles à partir des matières premières
• A01N 65/48 - Zingiberaceae [famille du gingembre], p. ex. gingembre ou grand galanga
• A01P 3/00 - Fongicides

Abrégé

A method for extracting Alpinia oxyphylla Miq essential oil for inhibiting Listeria monocytogenes, and Alpinia oxyphylla Miq essential oil. The method comprises the following steps: taking Alpinia oxyphylla Miq, drying mature fruits, crushing same, and sieving to obtain a powder; and mixing the powder with water according to a material-liquid mass ratio of 1:10-12, standing still and soaking for 2-3 h, and then performing steam distillation at 220-300°C for 5-7 h. The high-efficiency extraction of the essential oil is achieved by a steam distillation method, and an extraction rate reaches 1.5% or more. The obtained Alpinia oxyphylla Miq essential oil shows extremely strong inhibitory activity on Listeria monocytogenes. The inhibition zone diameter reaches 8.6 mm or more, and the MIC reaches 15 μL/mL or less.

Classes IPC  ?

• C11B 9/02 - Obtention ou raffinage des huiles essentielles à partir des matières premières
• A01N 65/00 - Biocides, produits repoussant ou attirant les animaux nuisibles, ou régulateurs de croissance des végétaux contenant du matériel provenant d'algues, de lichens, de bryophytes, de champignons multicellulaires ou de plantes, ou leurs extraits
• A01N 65/48 - Zingiberaceae [famille du gingembre], p. ex. gingembre ou grand galanga

Abrégé

A method for extracting galangal essential oil inhibiting Listeria monocytogenes. The method comprises the following steps: step 1, crushing: taking galangal rhizomes for crushing and sieving with a 30-50-mesh sieve to obtain galangal powder; step 2, primary extraction: adding distilled water of which the mass is 9-10 times that of the galangal powder into the galangal powder, soaking for 1-1.2 hours, and obtaining a galangal powder extraction mixture; and step 3, steam distillation: performing steam distillation on the galangal powder extraction mixture obtained in the step 2 at 220-228°C for 3-5 hours, and collecting an oil component to obtain the galangal essential oil. The extraction rate of the galangal rhizome essential oil under the extraction condition can be 1.006% or above, the galangal rhizome essential oil has a certain bacteriostatic effect on Listeria monocytogenes, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) thereof can both be below 25 μL/mL.

Classes IPC  ?

• C11B 9/02 - Obtention ou raffinage des huiles essentielles à partir des matières premières
• A61K 36/9062 - Alpinia, p. ex. Alipinia purpurata ou grand galanga
• A61P 31/04 - Agents antibactériens

Abrégé

An application of Alpinia officinarum Hance essential oil in preparation of bacteriostatic drugs against Klebsiella pneumoniae. Extracted Alpinia officinarum Hance essential oil has remarkable bacteriostatic activity on Klebsiella pneumoniae, and the minimum inhibitory concentration thereof can reach 0.391 μL/mL. The Alpinia officinarum Hance essential oil can be effectively applied to bacteriostatic drugs against Klebsiella pneumoniae.

Classes IPC  ?

• A61K 36/9062 - Alpinia, p. ex. Alipinia purpurata ou grand galanga
• A61P 31/04 - Agents antibactériens
• A61K 125/00 - Parties de plantes contenant des racines, des bulbes, des tubercules ou des rhizomes, ou obtenues à partir de ces éléments

Abrégé

A southern medicine compound toothpaste specifically for middle-aged and elderly people, and a preparation method therefor, which belong to the technical field of toothpaste processing. In accordance with the characteristics of the oral cavity of middle-aged and elderly people, and by means of multiple experimental screening, it is found that volatile oils of four southern medicines, which include Alpiniae officinarum rhizoma, Amomum longiligulare, Galangae fructus and Blumea balsamifera, are added to a toothpaste, so that the toothpaste has good antibacterial and anti-inflammatory effects. Moreover, the problem of the stability of the toothpaste being poor due to a high dosage being required when using a single Chinese herbal medicine, or the usability of the toothpaste being affected due to a pungent smell or a strong medicine flavor, is solved; and glycyrrhizic acid, citric acid and zinc citrate are compounded as a compound functional substance, so that the bacteriostasis performance and the desensitization effect of the toothpaste are enhanced. The southern medicine compound toothpaste specifically for middle-aged and elderly people does not damage dentin, has good cleanliness, is antibacterial, anti-inflammatory, anti-allergy, and is refreshing and comfortable. In addition, the toothpaste is suitable regarding the characteristics of susceptibility to allergies, a high incidence of bacterial caries, and common periodontitis and odontitis of the teeth of middle-aged and elderly people, has a clean, fine and smooth paste, moderate foam, is easy to extrude, has a good stability, and is easy to popularize and apply.

Classes IPC  ?

• A61K 8/92 - Huiles, graisses ou ciresLeurs dérivés, p. ex. produits d'hydrogénation
• A61K 8/63 - StéroïdesLeurs dérivés
• A61K 8/365 - Acides hydroxycarboxyliquesAcides cétocarboxyliques
• A61K 8/27 - ZincSes composés
• A61K 8/26 - AluminiumSes composés
• A61K 8/25 - SiliciumSes composés
• A61K 8/34 - Alcools
• A61K 8/24 - PhosphoreSes composés
• A61K 8/46 - Cosmétiques ou préparations similaires pour la toilette caractérisés par la composition contenant des composés organiques contenant du soufre
• A61K 8/44 - Acides aminocarboxyliques ou leurs dérivés, p. ex. acides aminocarboxyliques contenant du soufreLeurs sels, esters ou dérivés N-acylés
• A61Q 11/00 - Préparations pour le nettoyage des dents, de la bouche ou des prothèses dentaires, p. ex. dentifricesBains de bouche
• A61Q 17/00 - Préparations protectricesPréparations employées en contact direct avec la peau pour protéger des influences extérieures, p. ex. des rayons du soleil, des rayons X ou d'autres rayons nuisibles, des matériaux corrosifs, des bactéries ou des piqûres d'insectes
• A61P 1/02 - Préparations stomatologiques, p. ex. médicaments pour le traitement des caries, des aphtes, des périodontites
• A61P 37/08 - Agents antiallergiques