Disclosed herein are methods for making, culturing, and maintaining engineered podocyte-like cells. The methods comprise growing glomerular cells in in vitro conditions to differentiate the glomerular cells into podocyte-like cells. Also disclosed herein are isolated and purified podocyte-like cells and kits comprising the engineered podocyte-like cells and/or the media used to differentiate the podocyte-like cells. The engineered podocyte-like cells can be used in recellularization of a decellularized extracellular matrix. In some instances, disclosed herein are recellularized isolated organs that can comprise the engineered podocyte-like cells.
Mayo Foundation for Medical Education and Research (USA)
Inventeur(s)
Ross, Jeffrey
Young, Anne
Haarstad, Allie
Nyberg, Scott
Abrégé
The disclosure provides a method of using blood or fractions thereof, e.g., serum, obtained from a mammal subjected to liver surgery, for example, obtained following a partial hepatectomy, to increase the engraftment, proliferation and/or functionality of cells on a biocompatible scaffold.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
Provided herein are methods and compositions relating to at least partially recellularized human organs. Various methods of decellularizing non-human animal organs and recellularizing a non-human animal extracellular matrix with cell compositions. Further provided are compositions and methods for treating a liver or other disease (such as acute liver failure) using an extracorporeal bioengineered liver or other organ.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
A system (200) for growing or supporting an organ (50), in particular a biologically engineered organ, comprises: an enclosure (202); a perfusate circuit: a perfusate pump (206); a gas transfer unit (208) and a optionally a gas mixture unit connected to the gas transfer unit; one or more sensors (212a, 212b); an injection system (216) configured to inject nutrients; and a controller (214). The controller can be configured to operate the pump, the gas transfer unit, and the injection system based on sensor signals from the sensor(s) to grow or support the organ in an automated or semi-automated fashion.
Disclosed herein are methods to maintain or inhibit an alteration in volume or shape of hydrated decellularized extracellular matrix from a mammalian organ or tissue or a portion thereof.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
in vitroin vitro conditions to differentiate the glomerular cells into podocyte-like cells. Also disclosed herein are isolated and purified podocyte-like cells and kits comprising the engineered podocyte-like cells and/or the media used to differentiate the podocyte-like cells. The engineered podocyte-like cells can be used in recellularization of a decellularized extracellular matrix. In some instances, disclosed herein are recellularized isolated organs that can comprise the engineered podocyte-like cells.
G01N 33/554 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques le support étant une cellule ou un fragment de cellule biologique, p. ex. cellules de bactéries, de levure
The invention provides a method to prepare a graft comprising a recellularized extracellular matrix of a mammalian liver, liver lobe or portion thereof, and a method of using the recellularized extracellular matrix of a mammalian liver, liver lobe or portion thereof.
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
A61L 27/40 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent
A61L 27/54 - Matériaux biologiquement actifs, p. ex. substances thérapeutiques
A61L 27/58 - Matériaux au moins partiellement résorbables par le corps
05 - Produits pharmaceutiques, vétérinaires et hygièniques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Biological implants, namely, a vital processed human or animal connective tissue, namely, products derived from organ tissue, namely, porcine, bovine and human organ tissue, for use in surgical and non-surgical repair, enhancement and replacement of human tissue, namely, abdominal wall and hernia repair, breast reconstruction, cardiac repair, wound repair, liver, kidney, pancreas, heart, lung and bone repair Medical and scientific research; product development services in the fields of biotechnology, pharmaceuticals, and medical devices
A system (200) for supporting a patient organ can include a primary circuit (212b), a secondary circuit (212a), and a controller (214). The primary circuit can include an inlet configured to connect to the patient (54), an outlet configured to connect to the patient, and a primary pump (206a). The secondary circuit can be connected to the primary blood circuit and can include an enclosure (202) configured to support an organ (50) therein in a blood flow, a secondary pump, a gas transfer unit (208), and a secondary sensor (238a). The controller can be configured to operate the gas transfer unit based on the secondary sensor signal. The organ may be a bio-engineered organ. In an example, the organ is a liver and operates to perform functions of a liver in place of or in support of the liver of the patient.
A system (200) for growing or supporting an organ (50), in particular a biologically engineered organ, comprises: an enclosure (202); a perfusate circuit; a perfusate pump (206); a gas transfer unit (208) and a optionally a gas mixture unit connected to the gas transfer unit; one or more sensors (212a, 212b); an injection system (216) configured to inject nutrients; and a controller (214). The controller can be configured to operate the pump, the gas transfer unit, and the injection system based on sensor signals from the sensor(s) to grow or support the organ in an automated or semi-automated fashion.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
A system (200) for supporting a patient organ can include a primary circuit (212b), a secondary circuit (212a), and a controller (214). The primary circuit can include an inlet configured to connect to the patient (54), an outlet configured to connect to the patient, and a primary pump (206a). The secondary circuit can be connected to the primary blood circuit and can include an enclosure (202) configured to support an organ (50) therein in a blood flow, a secondary pump, a gas transfer unit (208), and a secondary sensor (238a). The controller can be configured to operate the gas transfer unit based on the secondary sensor signal. The organ may be a bio-engineered organ. In an example, the organ is a liver and operates to perform functions of a liver in place of or in support of the liver of the patient.
A system (200) for growing or supporting an organ (50), in particular a biologically engineered organ, comprises: an enclosure (202); a perfusate circuit; a perfusate pump (206); a gas transfer unit (208) and a optionally a gas mixture unit connected to the gas transfer unit; one or more sensors (212a, 212b); an injection system (216) configured to inject nutrients; and a controller (214). The controller can be configured to operate the pump, the gas transfer unit, and the injection system based on sensor signals from the sensor(s) to grow or support the organ in an automated or semi-automated fashion.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
14.
Methods of Decellularization and Recellularization of Organs and Portions of Organs
Disclosed herein are compositions and methods to decellularize an isolated organ or portion thereof. Also disclosed herein are compositions and methods for treatment of disease utilizing a decellularized or recellularized organ. Also disclosed herein are methods of improving decellularization and/or recellularization of an isolated organ or portion thereof.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
15.
Use of resected liver serum for whole liver-engineering
Mayo Foundation for Medical Education & Research, Mayo Medical Ventures (USA)
Inventeur(s)
Ross, Jeffrey
Young, Anne
Haarstad, Allie
Nyberg, Scott
Abrégé
The disclosure provides a method of using blood or fractions thereof, e.g., serum, obtained from a mammal subjected to liver surgery, for example, obtained following a partial hepatectomy, to increase the engraftment, proliferation and/or functionality of cells on a biocompatible scaffold.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
The disclosure provides for methods of sealing of decellularized and recellularized engineered organ grafts, thereby providing a reinforced (fortified) decellularized and recellularized engineered organ graft.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
Disclosed herein are compositions and methods to decellularize an isolated organ or portion thereof. Also disclosed herein are compositions and methods for treatment of disease utilizing a decellularized or recellularized organ. Also disclosed herein are methods of improving decellularization and/or recellularization of an isolated organ or portion thereof.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
The invention provides for methods and materials to decellularize an organ or portion thereof and to recellularize such a decellularized organ or portion thereof to thereby generate an organ or portion thereof.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
A61L 27/54 - Matériaux biologiquement actifs, p. ex. substances thérapeutiques
Disclosed herein are recellularized livers prepared from decellularized liver extracellular matrices. Also disclosed herein are kits and systems comprising a recellularized liver as described herein. Also disclosed herein are methods of recellularizing livers from decellularized liver extracellular matrices.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
A system for testing and transporting a biologically engineered organ can include a housing, a transportable enclosure, a perfusate circuit, and a pump. The housing can be configured to receive and support a biologically engineered organ therein in a perfusate flow. The housing can include a perfusate inlet and a perfusate outlet to receive the perfusate flow through the housing. The transportable enclosure can surround at least a portion of the housing. The perfusate circuit can be connected to the perfusate inlet and the perfusate outlet and can be configured to transmit perfusate through the system. The pump can be connected to the circuit and can be configured to circulate perfusate through the perfusate circuit.
Disclosed herein are methods for retention of shape of decellularized tissue or of a portion of an organ can be accomplished through the irradiation of the decellularized tissue or the portion of the organ inside a shaping mold. The enclosure of decellularized tissue or a portion of an organ inside of a mold or other constraining material, such as stainless steel or platinum or polymers such as polytetrafluoroethylene (PTFE) or polycaprolactone (PCL), allows the tissue to take on the shape of the mold or constraint and subsequently retain that shape after it is irradiated. This can result in decellularized extracellular matrix having defined (pre-determined) shapes. The system can include a hollow device which contains the filler or plug. The system may be inserted into the fistula tract and the filler or plug may be deployed by pulling, pushing or otherwise expelling the filler or plug into the tract.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
Disclosed herein are compositions and methods to decellularize an isolated organ or portion thereof. Also disclosed herein are compositions and methods for treatment of disease utilizing a decellularized or recellularized organ. Also disclosed herein are methods of improving decellularization and/or recellularization of an isolated organ or portion thereof.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
25.
SEALING OF DECELLULARIZED AND RECELLULARIZED ENGINEERED ORGAN GRAFTS
The disclosure provides for methods of sealing of decellularized and recellularized engineered organ grafts, thereby providing a reinforced (fortified) decellularized and recellularized engineered organ graft.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
Disclosed herein are compositions and methods to decellularize an isolated organ or portion thereof. Also disclosed herein are compositions and methods for treatment of disease utilizing a decellularized or recellularized organ. Also disclosed herein are methods of improving decellularization and/or recellularization of an isolated organ or portion thereof.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
31.
METHOD OF DEHYDRATION OF EXTRACELLULAR MATRIX AND PARTICLES FORMED THEREFROM
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
Disclosed herein are methods for retention of shape of decellularized tissue or of a portion of an organ can be accomplished through the irradiation of the decellularized tissue or the portion of the organ inside a shaping mold. The enclosure of decellularized tissue or a portion of an organ inside of a mold or other constraining material, such as stainless steel or platinum or polymers such as polytetrafluoroethylene (PTFE) or polycaprolactone (PCL), allows the tissue to take on the shape of the mold or constraint and subsequently retain that shape after it is irradiated. This can result in decellularized extracellular matrix having defined (pre-determined) shapes. The system can include a hollow device which contains the filler or plug. The system may be inserted into the fistula tract and the filler or plug may be deployed by pulling, pushing or otherwise expelling the filler or plug into the tract.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
A61L 2/08 - Procédés ou appareils de désinfection ou de stérilisation de matériaux ou d'objets autres que les denrées alimentaires ou les lentilles de contactAccessoires à cet effet utilisant des phénomènes physiques des radiations
A61L 27/40 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent
A61L 27/50 - Matériaux caractérisés par leur fonction ou leurs propriétés physiques
33.
Use of perfusion decellularized liver for islet cell recellularization
The invention provides a method to prepare a graft comprising a recellularized extracellular matrix of a mammalian liver, liver lobe or portion thereof, and a method of using the recellularized extracellular matrix of a mammalian liver, liver lobe or portion thereof.
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
A61L 27/54 - Matériaux biologiquement actifs, p. ex. substances thérapeutiques
A61L 27/58 - Matériaux au moins partiellement résorbables par le corps
A61L 27/40 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent
Disclosed herein are recellularized livers prepared from decellularized liver extracellular matrices. Also disclosed herein are kits and systems comprising a recellularized liver as described herein. Also disclosed herein are methods of recellularizing livers from decellularized liver extracellular matrices.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH (USA)
Inventeur(s)
Ross, Jeffrey
Young, Anne
Haarstad, Allie
Nyberg, Scott
Abrégé
The disclosure provides a method of using blood or fractions thereof, e.g., serum, obtained from a mammal subjected to liver surgery, for example, obtained following a partial hepatectomy, to increase the engraftment, proliferation and/or functionality of cells on a biocompatible scaffold.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
Mayo Foundation for Medical Education and Research (USA)
Miromatrix Medical Inc. (USA)
Inventeur(s)
Ross, Jeffrey
Young, Anne
Haarstad, Allie
Nyberg, Scott
Abrégé
The disclosure provides a method of using blood or fractions thereof, e.g., serum, obtained from a mammal subjected to liver surgery, for example, obtained following a partial hepatectomy, to increase the engraftment, proliferation and/or functionality of cells on a biocompatible scaffold.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
A method to provide an inflated decellularized extracellular matrix of a mammalian organ or a vascularized portion thereof, or a mammalian vascularized tissue or a vascularized portion thereof, an inflated decellularized extracellular matrix of a mammalian organ or a vascularized portion thereof, or a mammalian vascularized tissue or a vascularized portion thereof, and uses thereof, are provided.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
A method to provide an inflated decellularized extracellular matrix of a mammalian organ or a vascularized portion thereof, or a mammalian vascularized tissue or a vascularized portion thereof, an inflated decellularized extracellular matrix of a mammalian organ or a vascularized portion thereof, or a mammalian vascularized tissue or a vascularized portion thereof, and uses thereof, are provided.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
The invention provides a method for preparing a perfusion based islet cell containing extracellular matrix of liver, an islet cell recellularized extracellular matrix, and methods of using the recellularized matrix.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
A61L 27/40 - Matériaux composites, c.-à-d. en couches ou contenant un matériau dispersé dans une matrice constituée d'un matériau analogue ou différent
A61L 27/54 - Matériaux biologiquement actifs, p. ex. substances thérapeutiques
A61L 27/58 - Matériaux au moins partiellement résorbables par le corps
42.
USE OF MICROPARTICLES AND ENDOTHELIAL CELLS WITH DECELLULARIZED ORGANS AND TISSUES
The invention provides a method for maintaining capillary lumen diameter, reducing a decrease in capillary vessel lumen diameter or expanding capillary vessel lumen diameter in a re-endothelialized decellularized organ or tissue graft with an intact extracellular matrix vascular network. The method is based on administration of endothelial cells and microparticles to the decellularized ECM.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
The invention provides a method for maintaining capillary lumen diameter, reducing a decrease in capillary vessel lumen diameter or expanding capillary vessel lumen diameter in a re-endothelialised but otherwise decellularised organ or tissue graft with an intact extracellular matrix (ECM) vascular network. The method is based on administration of endothelial cells and microparticles to the decellularised ECM.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
44.
USE OF PERFUSION DECELLULARIZED LIVER FOR ISLET CELL RECELLULARIZATION
The invention provides a method for preparing a perfusion based islet cell containing extracellular matrix of liver, an islet cell recellularized extracellular matrix, and methods of using the recellularized matrix.
The invention provides a method for preparing a perfusion based islet cell containing extracellular matrix of liver, an islet cell recellularized extracellular matrix, and methods of using the recellularized matrix.
The invention provides for methods and materials to decellularize a solid organ and to recellularize such a decellularized organ to thereby generate a solid organ.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
A61K 35/12 - Substances provenant de mammifèresCompositions comprenant des tissus ou des cellules non spécifiésCompositions comprenant des cellules souches non embryonnairesCellules génétiquement modifiées
A61K 9/00 - Préparations médicinales caractérisées par un aspect particulier
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
The invention provides for methods and materials to decellularize a solid organ and to recellularize such a decellularized organ to thereby generate a solid organ.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
A61K 35/12 - Substances provenant de mammifèresCompositions comprenant des tissus ou des cellules non spécifiésCompositions comprenant des cellules souches non embryonnairesCellules génétiquement modifiées
A61K 9/00 - Préparations médicinales caractérisées par un aspect particulier
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
The invention provides a method for preparing a perfusion based 3D cell culture system, a recellularized matrix culture system, and methods of using the culture system.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
52.
DECELLULARIZATION AND RECELLULARIZATION OF ORGANS AND TISSUES
The invention provides for methods and materials to decellularize an organ or portion thereof and to recellularize such a decellularized organ or portion thereof to thereby generate an organ or portion thereof.
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
The invention provides for methods and materials to decellularize a solid organ and to recellularize such a decellularized organ to thereby generate a solid organ.
The invention provides for methods and materials to decellularize a solid organ and to recellularize such a decellularized organ to thereby generate a solid organ.
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
A61L 27/36 - Matériaux pour prothèses ou pour revêtement de prothèses contenant des constituants de constitution indéterminée ou leurs produits réactionnels
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
