An EP6 promoter, a related biomaterial, and use thereof are provided. The sequence of the EP6 promoter is set forth in SEQ ID NO: 3 in the sequence listing. The EP6 promoter is used as an exogenous strong promoter to be integrated into bacteria with high-yield amino acids to replace an original promoter of key genes for amino acid synthesis, so that the yield of a corresponding amino acid is improved.
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
An NCg12747 gene mutant and the use thereof in the preparation of L-lysine are provided. The NCg12747 gene mutant is a DNA molecule shown in SEQ ID NO: 3, which encodes the NCg12747 mutant protein shown in SEQ ID NO: 4. Mutating the NCg12747 gene into the NCg12747 gene mutant shown in SEQ ID NO: 3 or overexpressing NCg12747 gene mutant contributes to increase in the yield and the growth rate of L-lysine. However, when the gene is weakened or knocked out, accumulation of the L-lysine is not facilitated, and the growth rate of a strain can be reduced. The NCg12747 gene mutant and the NCg12747 mutant protein encoded thereby can be used for preparing L-lysine.
C07K 14/34 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Corynebacterium (G)
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
30 - Aliments de base, thé, café, pâtisseries et confiseries
Produits et services
Fruit flavourings for food and beverages; Flavored sugar; Food flavorings; almond extracts for use as a food flavoring; Food flavorings, namely, infused oils; Seasonings; Seasoning mixes; Seasoning mixes for soups; Food seasonings; Condiment, namely, pepper sauce; Condiment, namely, oyster sauce; Seasoned salt; Seasoning pastes; Monosodium glutamate used as a flavor enhancer for food; Yeast; Yeast extracts for food; Yeast extracts; Soy sauce; Savory sauces used as condiments; Food flavourings and seasonings
30 - Aliments de base, thé, café, pâtisseries et confiseries
Produits et services
Fruit flavourings for food and beverages; Flavored sugar; Food flavorings; almond extracts for use as a food flavoring; Food flavorings, namely, infused oils; Seasonings; Seasoning mixes; Seasoning mixes for soups; Food seasonings; Condiment, namely, pepper sauce; Condiment, namely, oyster sauce; Seasoned salt; Seasoning pastes; Monosodium glutamate used as a flavor enhancer for food; Yeast; Yeast extracts for food; Yeast extracts; Soy sauce; Savory sauces used as condiments; Food flavourings and seasonings
Provided are a galactose-1-phosphate uridyltransferase mutant and the use thereof in the preparation of L-lysine. The galactose-1-phosphate uridyltransferase is A1) or A2): A1) a protein having an amino acid sequence as shown in SEQ ID No. 2; and A2) a fusion protein obtained by linking a tag to the N-terminal or/and the C-terminal of A1). Terminating or knocking out coding genes of the galactose-1-phosphate uridyltransferase in advance can improve the yield of L-lysine in cells.
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
The present invention relates to the technical field of biology, and disclosed is a use of NCgl0762 protein and a mutant thereof in the production of L-isoleucine. The technical problem solved by the present invention is how to increase the yield of L-isoleucine. The sequence of a protein disclosed in the present invention is SEQ ID No. 4 or SEQ ID No. 2. Replacing a coding gene of the protein shown in SEQ ID No. 2 in a starting biological cell with a coding gene of the protein shown in SEQ ID No. 4, or increasing the content or activity of the protein shown in SEQ ID No. 2 or SEQ ID No. 4 in the starting biological cell, can both increase the yield of L-isoleucine.
C07K 14/34 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Corynebacterium (G)
C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
The present invention belongs to the field of biotechnology. Provided are promoter EP11, and a related biological material thereof and the use thereof. The technical problem to be solved is how to increase the content of L-lysine. The provided promoter EP11 has a sequence of SEQ ID No. 1 in the sequence listing. The EP11 promoter is introduced into Corynebacterium glutamicum capable of synthesizing L-lysine, such that the EP11 promoter drives the expression of genes in the L-lysine synthesis pathway, thereby obtaining recombinant Corynebacterium glutamicum. The recombinant Corynebacterium glutamicum is cultured to obtain L-lysine, and the yield of the L-lysine is increased. It is indicated that the provided EP11 promoter not only has a promoter activity, but can also be used for producing L-lysine.
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
A promoter EP10 containing a sequence as set forth in SEQ ID NO. 1. The promoter EP10 is introduced into a biological cell capable of synthesizing L-lysine, such that the promoter EP10 drives the expression of genes in the L-lysine synthesis pathway in the biological cell, thereby obtaining a recombinant biological cell. The recombinant biological cell is cultured to obtain L-lysine, and the yield of the L-lysine is increased.
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
C12N 15/67 - Méthodes générales pour favoriser l'expression
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
A modified Escherichia coli and the use thereof in the production of L-amino acid by means of fermentation. Specifically, disclosed is the use of pyruvate formate lyase, alcohol dehydrogenase, branched-chain amino acid transaminase derived from Escherichia coli, branched-chain amino acid transaminase derived from Bacillus subtilis, thiamine phosphate synthase and malate dehydrogenase in the construction of a genetically engineered bacterium for producing L-amino acid. Experiments prove that the recombinant bacterium, which is conducive to the accumulation of L-amino acid, is obtained by means of knocking out the encoding genes of the pyruvate formate lyase, the alcohol dehydrogenase, the branched-chain amino acid transaminase derived from Escherichia coli, the thiamine phosphate synthase and the malate dehydrogenase of Escherichia coli capable of producing L-amino acid, and introducing the encoding gene of the branched-chain amino acid transaminase derived from Bacillus subtilis. The recombinant bacteria can greatly increase the yield of L-amino acid and reduce the costs, and is of great significance to accelerate the industrial progress of L-amino acid.
Disclosed in the present invention are a promoter, and a related biological material thereof and a use thereof. The present invention provides a DNA molecule, which can be used as a promoter. Also provided are an expression cassette, a recombinant vector, a recombinant host cell, or a recombinant microorganism, each containing the DNA molecule. Also provided is a method for using the DNA molecule to produce an L-amino acid or increase the content of an L-amino acid in bacteria.
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C07K 14/34 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Corynebacterium (G)
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
Disclosed is the use of a PNcgl1855 (A244C) promoter and a biomaterial related thereto in preparation of L-lysine. The promoter is a DNA molecule obtained by mutating adenine at position 244 of a PNcgl1855 promoter into cytosine. L-lysine fermentation experiments performed on constructed engineered strains, i.e. L-PNcgl1855 and Y-PNcgl1855, containing the PNcgl1855 (A244C) promoter show that introducing a point mutation into the promoter region of the Ncgl1855 gene in Corynebacterium glutamicum contributes to an increase in the yield of L-lysine. The present invention is of great significance for increasing the yield of L-lysine in bacterial fermentation.
C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
Disclosed is the use of a PNcgl1969(C200T) promoter and a biomaterial related thereto in the construction of a high-yield L-lysine engineered strain. The promoter is a DNA molecule obtained by means of mutating cytosine at position 200 in the PNcgl1969 promoter to thymine. By means of performing L-lysine fermentation experiments on the constructed engineered strains L-PNcgl1969 and Y-PNcgl1969 containing the PNcgl1969(C200T) promoter, it is found that a point mutation of the PNcgl1969 promoter region in Corynebacterium glutamicum is conducive to improving the yield of L-lysine. The present invention is of great significance for increasing the yield of L-lysine produced by means of bacterial fermentation.
C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
Disclosed is a use of α-glucosidase gene and α-glucosidase or a substance for regulating the activity and/or content of α-glucosidase in the preparation of L-amino acids.
C12N 9/26 - Hydrolases (3.) agissant sur les composés glycosyliques (3.2) agissant sur les liaisons alpha-glucosidiques-1, 4, p. ex. hyaluronidase, invertase, amylase
C12N 15/56 - Hydrolases (3) agissant sur les composés glycosyliques (3.2), p. ex. amylase, galactosidase, lysozyme
C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
Provided is the use of a cell death-related protein in preparing L-lysine. Further provided is a bacterial strain capable of reducing toxic substances or eliminating foams during a fermentation process, so as to produce L-lysine with a high yield. In order to solve the technical problem, the described use is the use of a protein, or a substance for regulating the expression of a coding gene of the protein, or a substance for regulating the activity or content of the protein, in preparing L-lysine or improving the yield of L-lysine, the protein being specifically one or more of SEQ ID No. 2, SEQ ID No. 4, SEQ ID No. 7, SEQ ID No. 10 and SEQ ID No. 12. Experiments prove that knocking out the coding gene of the described protein helps to improve the yield of L-lysine in the bacterial strain.
C07K 14/34 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Corynebacterium (G)
C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
21.
METHOD FOR PRODUCING L-ARGININE USING RECOMBINANT ESCHERICHIA COLI, AND RECOMBINANT BACTERIUM USED THEREIN
A method for producing L-arginine using a recombinant Escherichia coli, and a recombinant bacterium used therein. Specifically, the present invention relates to a method for producing L-arginine using a recombinant Escherichia coli, and a recombinant bacterium used therein. The recombinant Escherichia coli is a recombinant bacterium obtained by means of over-expressing a gene encoding a cyanate transporter in a receptor Escherichia coli, or a recombinant bacterium obtained by means of over-expressing a gene encoding a carbonic anhydrase in a receptor Escherichia coli. The cyanate transporter is a protein having an amino acid sequence of SEQ ID NO: 4, and the carbonic anhydrase is a protein having an amino acid sequence of SEQ ID NO: 5. By means of regulating the expression of the cyanate transporter or carbonic anhydrase, the yield of arginine is significantly improved. The method provides a new line of thought for the synthesis of bio-based L-arginine, and has wide application space and market prospects.
A modified Escherichia coli and the use thereof in the production of L-amino acid by means of fermentation. Specifically, disclosed is the use of pyruvate formate lyase, alcohol dehydrogenase, branched-chain amino acid transaminase derived from Escherichia coli, branched-chain amino acid transaminase derived from Bacillus subtilis, thiamine phosphate synthase and malate dehydrogenase in the construction of a genetically engineered bacterium for producing L-amino acid. Experiments prove that the recombinant bacterium, which is conducive to the accumulation of L-amino acid, is obtained by means of knocking out the encoding genes of the pyruvate formate lyase, the alcohol dehydrogenase, the branched-chain amino acid transaminase derived from Escherichia coli, the thiamine phosphate synthase and the malate dehydrogenase of Escherichia coli capable of producing L-amino acid, and introducing the encoding gene of the branched-chain amino acid transaminase derived from Bacillus subtilis. The recombinant bacteria can greatly increase the yield of L-amino acid and reduce the costs, and is of great significance to accelerate the industrial progress of L-amino acid.
Provided are isocitrate lyase and a use thereof in preparation of L-amino acid. Specifically, provided is a use of a protein having an amino acid sequence of SEQ ID No. 2 or a substance for regulating the activity and/or content of the protein in constructing a genetically engineered bacterium for producing L-amino acid. An aceA gene mutant engineered strain obtained by introducing a point mutation (C-T) to position 1111 of an aceA gene coding region of an L-amino acid high-yield strain and wild-type Escherichia coli W3110, and an engineered strain obtained by knocking out an aceA gene are constructed. Experiments show that the aceA gene and a variant thereof such as an aceAQ371* gene participate in biosynthesis of L-amino acid, and knocking out or weakening the aceA gene facilitates accumulation of L-amino acid. The aceA gene and the variant thereof can be used to construct a genetically engineered strain for producing L-amino acid so as to promote the increase of the yield of L-amino acid.
Provided are a galactose-1-phosphate uridyltransferase mutant and the use thereof in the preparation of L-lysine. The galactose-1-phosphate uridyltransferase is A1) or A2): A1) a protein having an amino acid sequence as shown in SEQ ID No. 2; and A2) a fusion protein obtained by linking a tag to the N-terminal or/and the C-terminal of A1). Terminating or knocking out encoding genes of the galactose-1-phosphate uridyltransferase in advance can improve the yield of L-lysine in cells.
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
Provided are a recombinant strain with modified gene BBD29_14900, and a method for constructing the same and use thereof, with the production of L-glutamic acid as a specific application. Further provided is a method for introducing a point mutation into the BBD29_14900 gene coding sequence in Corynebacterium or improving the expression thereof. The method can cause a bacterial strain with the mutation to increase the fermentation yield of glutamic acid. The point mutation involves a mutation of the base at position 1114 in the sequence of the BBD29_14900 gene from guanine (G) to adenine (A), and thus a substitution of aspartic acid at position 372 in the coded corresponding amino acid sequence with asparagine.
C12P 13/18 - Acide glutamiqueGlutamine utilisant la biotine ou ses dérivés
C07K 14/34 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Corynebacterium (G)
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
26.
RECOMBINANT STRAIN WITH MODIFIED GENE BBD29_11265 FOR PRODUCING L-GLUTAMIC ACID, AND METHOD FOR CONSTRUCTING THE SAME AND USE THEREOF
A recombinant strain with modified gene BBD29_11265 and a method for constructing the same are provided. The recombinant strain is a bacterium that generates L-glutamic acid, and has an improved expression of a polynucleotide encoding an amino acid sequence of SEQ ID NO: 3 or a homologous sequence thereof; the improved expression can be having a point mutation in, and an enhanced expression of the polynucleotide encoding an amino acid sequence of SEQ ID NO: 3 or a homologous sequence thereof. A genetically engineered bacterium in which the base at position 70 in the BBD29_112665 gene sequence is mutated to adenine from guanine, causing alanine at position 24 in the coded corresponding amino acid sequence to be substituted with threonine, and an engineered bacterium overexpressing the BBD29_112665 gene or BBD29_11265G70A gene are constructed in the present invention, facilitating an increase in the production and conversion rate of L-glutamic acid.
C12P 13/18 - Acide glutamiqueGlutamine utilisant la biotine ou ses dérivés
C07K 14/34 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Corynebacterium (G)
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
27.
STRAIN HAVING ENHANCED L-GLUTAMIC ACID PRODUCTIVITY, CONSTRUCTION METHOD THEREFOR AND APPLICATION THEREOF
Disclosed are strain having enhanced L-glutamic acid production capacity, and method for constructing the same and use thereof. A nucleotide sequence is provided by introducing a point mutation to a wild-type BBD29-00405 gene in Corynebacterium glutamicum so that the base at position 597 of SEQ ID NO: 1 is mutated from guanine (G) into adenine (A). Also provided is a recombinant strain obtained by introducing the polynucleotide sequence into L-glutamic acid-producing Corynebacterium glutamicum, the recombinant strain comprising a BBD29-00405 gene containing a point mutation. Compared with an unmodified strain, the resulting strain facilitates production of L-glutamic acid at a higher concentration.
C07K 14/34 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Corynebacterium (G)
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
28.
EP6 PROMOTER, RELATED BIOMATERIAL, AND USE THEREOF
Provided are an EP6 promoter, a related biomaterial, and use thereof. The sequence of the EP6 promoter is set forth in SEQ ID No. 3 in the sequence listing. The EP6 promoter is used as an exogenous strong promoter to be integrated into bacteria with high-yield amino acids to replace an original promoter of key genes for amino acid synthesis, so that the yield of a corresponding amino acid is improved.
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
Provided are an NCgl2747 gene mutant and the use thereof in the preparation of L-lysine. The NCgl2747 gene mutant is a DNA molecule shown in SEQ ID No.3, which encodes the NCgl2747 mutant protein shown in SEQ ID No.4. Mutating the NCgl2747 gene into the NCgl2747 gene mutant shown in SEQ ID No.3 or overexpressing NCgl2747 gene mutant contributes to increase in the yield and the growth rate of L-lysine. However, when the gene is weakened or knocked out, accumulation of the L-lysine is not facilitated, and the growth rate of a strain can be reduced. The NCgl2747 gene mutant and the NCgl2747 mutant protein encoded thereby can be used for preparing L-lysine.
C07K 14/34 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Corynebacterium (G)
C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
Taking Corynebacterium glutamicum YP97158 as the starting bacterium, introducing site-directed mutation and/or expression enhancement in the coding region of its NCgl1089 gene, the coding region of NCgl0761 gene, and/or the coding region of ptsS gene, the obtained mutant gene and the recombination comprising said gene has high-efficiency L-amino acids production capacity, which greatly increases the output of L-amino acids, and the strain has good stability, which reduces the production cost as an L-amino acids production strain.
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
C07K 14/34 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Corynebacterium (G)
31.
Genetically engineered strain with high yield of L-valine and method for producing L-valine by fermentation
Escherichia coli. Furthermore, the L-valine fermentation method is improved by using a two-stage dissolved oxygen control. The L-valine titer and the sugar-acid conversion rate are increased.
E. coli and finally obtained a genetically engineered bacterial strain which has a clear genetic background, carries no plasmids, undergoes no mutagenesis and is capable of stably and efficiently producing L-arginine.
Tianjin University of Science & Technology (Chine)
Inventeur(s)
Xie, Xixian
Xiong, Bo
Zhao, Chunguang
Guo, Xiaowei
Men, Jiaxuan
Wei, Aiying
Abrégé
A transport protein coding gene, and a method for efficient production of L-tryptophan by a strain containing the gene. Specifically, by heterologous expression of ywkB gene from Bacillus subtilis on the genome of Escherichia coli, L-tryptophan production efficiency of the strain can be improved. Performing shake flask fermentation with the strain can accumulate 15.2 g/L of L-tryptophan within 24 h, which is 35% higher than a control strain.
The invention concerns a genetically modified microorganism expressing a functional type I or II RuBisCO enzyme and a functional phosphoribulokinase (PRK), and in which the glycolysis pathway is at least partially inhibited, said microorganism being genetically modified so as to produce an exogenous molecule and/or to overproduce an endogenous molecule. According to the invention, the oxidative branch of the pentose phosphate pathway may also be at least partially inhibited. The invention also concerns the use of such a genetically modified microorganism for the production or overproduction of a molecule of interest and processes for the synthesis or bioconversion of molecules of interest.
Disclosed in the present invention are a recombinant strain for producing L-glutamic acid by means of modifying gene BBD29_11265, and a construction method and the use thereof. The recombinant strain is a bacterium that generates L-glutamic acid, and has an improved expression of a polynucleotide encoding an amino acid sequence of SEQ ID NO: 3 or a homologous sequence thereof. The improved expression may be that the polynucleotide encoding an amino acid sequence of SEQ ID NO: 3 or a homologous sequence thereof has a point mutation and the expression thereof is enhanced. The genetically engineered bacterium in which the base at position 70 of the BBD29_112665 gene sequence is mutated into adenine from guanine and the alanine at position 24 of the encoded corresponding amino acid sequence is substituted with threonine, and the engineering bacterium overexpressing the BBD29_112665 gene or BBD29_11265 G70A gene are constructed in the present invention. The bacteria contribute to improving the yield and conversion rate of L-glutamic acid.
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
C07K 14/34 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Corynebacterium (G)
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
C12P 13/18 - Acide glutamiqueGlutamine utilisant la biotine ou ses dérivés
Provided are a recombinant strain of modifying gene BBD29_14900, and a construction method and the use thereof. The specific use thereof is for producing L-glutamic acid. Further provided is a method for introducing point mutation into a BBD29_14900 gene coding sequence in Corynebacterium or improving the expression thereof. The method can be used to increase the fermentation yield of glutamic acid by means of a bacterial strain with the mutation. According to the point mutation, the base at position 1114 of the BBD29_14900 gene sequence is mutated into adenine (A) from guanine (G), and the aspartic acid at position 372 of the coded corresponding amino acid sequence is substituted with asparagine.
C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
C07K 14/34 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Corynebacterium (G)
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
C12P 13/18 - Acide glutamiqueGlutamine utilisant la biotine ou ses dérivés
Disclosed are a strain having enhanced L-glutamic acid productivity, a construction method therefor, and an application thereof. Point mutation is introduced into a wild type BBD29-00405 gene in corynebacterium glutamicum, so that the 597th site base group of the nucleotide sequence of SEQ ID NO: 1 is obtained by mutating guanine (G) into adenine (A). Also provided is a recombinant strain obtained by introducing a polynucleotide sequence into the corynebacterium glutamicum to produce L-glutamic acid, the recombinant strain comprising a BBD29-00405 gene containing a point mutation. Compared with unmodified strains, the obtained strain is beneficial to producing high-concentration L-glutamic acid.
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
C07K 14/34 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Corynebacterium (G)
Corynebacterium bacterial chromosome with a EP5 promoter, and fermenting bacteria obtained by modification to produce L-lysine. Also provided are methods and applications derived from the method, and bacteria and promoter that can used in the methods and the applications.
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
Corynebacterium glutamicum YP97158 is used as a starting bacterium, and a site-specific mutagenesis and/or enhanced expression are introduced into the NCgl1089 gene coding region, the NCgl0761 gene coding region, and/or the ptsS gene coding region thereof. An obtained mutated gene and a recombinant strain including the gene have an highly efficient production capacity of L-amino acid, the yield of L-amino acid is greatly improved, the strain stability is good, and the production cost of same as an L-amino acid production strain is reduced.
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
C07K 14/34 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Corynebacterium (G)
Disclosed are gene engineering bacteria for producing L-arginine and a construction method and an application of the gene engineering bacteria. According to the method, a gene for encoding carbamyl phosphate synthetase and a gene for encoding an L-arginine biosynthetic pathway enzyme are integrated in Escherichia coli; a synthetic pathway of arginine in Escherichia coli and metabolic flux related to arginine in a whole amino acid metabolic network are analyzed and reconstructed to obtain gene engineering bacteria which are clear in genetic background, do not carry plasmids, are not mutated and can stably and efficiently produce L-arginine.
TIANJIN UNIVERSITY OF SCIENCE & TECHNOLOGY (Chine)
Inventeur(s)
Xie, Xixian
Xiong, Bo
Zhao, Chunguang
Guo, Xiaowei
Men, Jiaxuan
Wei, Aiying
Abrégé
A transport protein coding gene, and a method for using a strain containing said gene to increase L-tryptophan production. Specifically, strain L-tryptophan production efficiency can be improved by means of heterologous expression of the ywkB gene from bacillus subtilis on the escherichia coli genome. Performing shake flask fermentation with the strain can accumulate 15.2g/L of L-tryptophan within 24 hours, which is 35% higher than a control strain.
Corynebacterium bacterial chromosome with a EP5 promoter, and fermenting bacteria obtained by modification to produce L-lysine. Also provided are methods and applications derived from the method, and bacteria and promoter that can used in the methods and the applications.
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
INSTITUTE OF MICROBIOLOGY, CHINESE ACADEMY OF SCIENCES (Chine)
Inventeur(s)
Wen, Tingyi
Zhang, Chen
Shang, Xiuling
Chai, Xin
Zhang, Yun
Liu, Shuwen
Wang, Guoqiang
Li, Zhongcai
Abrégé
A recombinant bacterium for producing L-lysine, a construction method thereof, and a method for producing L-lysine by using the recombinant bacterium. The recombinant bacterium has increased expression and/or activity of asparaginase compared to a starting bacterium.
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 15/64 - Méthodes générales pour la préparation du vecteur, pour son introduction dans la cellule ou pour la sélection de l'hôte contenant le vecteur
C12N 15/69 - Augmentation du nombre de copies du vecteur
A method for producing L-lysine by fermentation, comprising modifying a gene for coding an NCBI reference sequence NP_601029.1 and/or NP_599350.1 on a Corynebacterium bacterial chromosome to enable the activity and/or expression quantity of NP_601029.1 and/or NP_599350.1 to be reduced; replacing a promoter of one or more genes on the Corynebacterium bacterial chromosome with a EP5 promoter; and fermenting bacteria obtained by modification to produce L-lysine. Also provided are methods and applications derived from the method, and bacteria and promoter that can be used in the methods and the applications.
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
A method for producing L-lysine by fermentation, comprising modifying a gene for coding an NCBI reference sequence NP_601029.1 and/or NP_599350.1 on a Corynebacterium bacterial chromosome to enable the activity and/or expression quantity of NP_601029.1 and/or NP_599350.1 to be reduced; replacing a promoter of one or more genes on the Corynebacterium bacterial chromosome with a EP5 promoter; and fermenting bacteria obtained by modification to produce L-lysine. Also provided are methods and applications derived from the method, and bacteria and promoter that can be used in the methods and the applications.
C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
A method for fermentation-production of a pentanediamine, comprising: culturing a cell expressing a lysine decarboxylase to obtain a whole cell fermentation broth comprising a pentanediamine; and extracting the pentanediamine from the whole cell fermentation broth, and stripping the whole cell fermentation broth of carbon dioxide contained therein before adding a strong base. The method greatly increases a production volume of the pentanediamine.
C12P 13/00 - Préparation de composés organiques contenant de l'azote
C12P 1/00 - Préparation de composés ou de compositions, non prévue dans les groupes , utilisant des micro-organismes ou des enzymesProcédés généraux de préparation de composés ou de compositions utilisant des micro-organismes ou des enzymes
Provided is an L-lysine generation method by fermentation, comprising modifying the aconitase gene and/or regulatory element thereof on a bacteria chromosome, such that the enzymatic activity and/or expression quantity of the aconitase are reduced but do not disappear; and generating L-lysine by fermenting the modified bacteria. Also disclosed are methods and uses derived from the present method, and the bacteria that can be used in these methods and uses.
Provided is an L-lysine generation method by fermentation, comprising modifying the aconitase gene and/or regulatory element thereof on a bacteria chromosome, such that the enzymatic activity and/or expression quantity of the aconitase are reduced but do not disappear; and generating L-lysine by fermenting the modified bacteria. Also disclosed are methods and uses derived from the present method, and the bacteria that can be used in these methods and uses.
