The present disclosure provides systems, methods, and apparatuses for improved magnetic separation of a biological population. A system for magnetic separation and collection of a target biological population from a biological sample comprises a cell engineering cassette, and a fluidic pathway disposed within the cell engineering cassette. The fluidic pathway has entrapment features disposed along a flow path of the fluidic pathway. An array of magnets is disposed adjacent to the fluidic pathway and the cell engineering cassette such that the array of magnets can be translatable toward and away from the fluidic pathway to apply a magnetic field to the biological population for the separation of a first subpopulation from a second subpopulation.
C12N 15/85 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules animales
C12N 15/90 - Introduction stable d'ADN étranger dans le chromosome
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
2.
APPARATUS, SYSTEM, AND METHOD FOR HIGH YIELD MAGNETIC SEPARATION OF A BIOLOGICAL POPULATION
The present disclosure provides systems, methods, and apparatuses for improved magnetic separation of a biological population. A system for magnetic separation and collection of a target biological population from a biological sample comprises a cell engineering cassette, and a fluidic pathway disposed within the cell engineering cassette. The fluidic pathway has entrapment features disposed along a flow path of the fluidic pathway. An array of magnets is disposed adjacent to the fluidic pathway and the cell engineering cassette such that the array of magnets can be translatable toward and away from the fluidic pathway to apply a magnetic field to the biological population for the separation of a first subpopulation from a second subpopulation.
Systems, devices and methods for automatic magnetic separation of magnetized targets in a biological sample are herein disclosed, where they comprise a magnetic field shield/barrier controllably operable to control the magnetic field in terms of reaching and attracting the magnetized targets within the biological sample.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
B03C 1/02 - Séparation magnétique agissant directement sur la substance à séparer
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12N 5/0783 - Cellules TCellules NKProgéniteurs de cellules T ou NK
4.
ENHANCED SEDIMENTATION MODULES AND SYSTEM FOR IMPROVED CELL CONCENTRATION
Systems, methods, and devices for particle separation are provided. A cell sedimentation module may include a housing, which may include a cell sedimentation vessel disposed within the housing, the cell sedimentation vessel having sidewalls, a bottom surface, and a cell collection well formed into the bottom surface. The sedimentation module may include a first flow line configured for input of a cell suspension and output of a supernatant. The sedimentation module may include a second flow line configured for output of a target cell suspension, wherein the cell sedimentation vessel is configured to non-mechanically generate a centripetal flow therein to facilitate separation of the supernatant and the target cell suspension from the cell suspension. In embodiments, the cell sedimentation module may incorporate multiple stacked vessels. In alternative embodiments, the cell sedimentation module may include at least one insert disposed therein to facilitate separation of supernatant and target cell suspension.
G01N 15/01 - Recherche de caractéristiques de particulesRecherche de la perméabilité, du volume des pores ou de l'aire superficielle effective de matériaux poreux spécialement adaptée aux cellules biologiques, p. ex. aux cellules sanguines
5.
ENHANCED SEDIMENTATION MODULES AND SYSTEM FOR IMPROVED CELL CONCENTRATION
Systems, methods, and devices for particle separation are provided. A cell sedimentation module may include a housing, which may include a cell sedimentation vessel disposed within the housing, the cell sedimentation vessel having sidewalls, a bottom surface, and a cell collection well formed into the bottom surface. The sedimentation module may include a first flow line configured for input of a cell suspension and output of a supernatant. The sedimentation module may include a second flow line configured for output of a target cell suspension, wherein the cell sedimentation vessel is configured to non-mechanically generate a centripetal flow therein to facilitate separation of the supernatant and the target cell suspension from the cell suspension. In embodiments, the cell sedimentation module may incorporate multiple stacked vessels. In alternative embodiments, the cell sedimentation module may include at least one insert disposed therein to facilitate separation of supernatant and target cell suspension.
The present disclosure provides methods for assessing and optimizing cellular quality of a cell-based therapy that is being produced in an automated cell engineering system. The methods suitably include monitoring molecular characteristics of the cells before, during, and after the automated process to provide feedback to the process parameters. In embodiments, the cells being produced are Chimeric Antigen Receptor (CAR) T-cells.
An automated cell culture and tissue engineering system comprising defined and separate environmental zones provide for increased control and maintenance of the internal environment of the system such that the temperature, air flow and gases surrounding the bioreactor module form one zone that is maintained separately to a second zone formed surrounding the reagent fluid reservoir. The system further comprises means for elimination and/or management of condensation within the second zone of the system.
C12M 1/02 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'agitationAppareillage pour l'enzymologie ou la microbiologie avec des moyens d'échange de chaleur
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Computer software for the management, monitoring, data
storage, and support of equipment for manufacturing
autologous cell therapies. Software as a service for the management, monitoring, data
storage, and support of equipment for manufacturing
autologous cell therapies.
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
(1) Computer software for the management, monitoring, data storage, and support of equipment for manufacturing autologous cell therapies. (1) Software as a service for the management, monitoring, data storage, and support of equipment for manufacturing autologous cell therapies.
The present disclosure provides combinatorial fluid switches that allow for the selection of a single fluid flow-path, while controlling multiple flow-paths. The combinatorial fluid switches can be used in various biological systems and processes, included automated cell engineering systems.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.
C07K 14/705 - RécepteursAntigènes de surface cellulaireDéterminants de surface cellulaire
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
The present disclosure provides cell culture chambers for use in automated cell engineering systems, and in particular, cell culture chambers that provide increased cell yield. A cell culture chamber may include a first body forming a first portion of the cell culture chamber. A cell culture chamber may include a second body forming a second portion of the cell culture chamber, the first body and the second body configured to couple together, forming an enclosed volume, wherein a first non-porous, gas-permeable material is disposed on the first body; and wherein the first non-porous, gas-permeable material and the first body are formed together. A second non-porous, gas-permeable material may be disposed on the second body, and the second non-porous, gas-permeable material and the second body may be formed together. The cell culture chamber may further include at least one bubble trap integrally formed with the first body and/or the second body.
The present disclosure provides cell culture chambers for use in automated cell engineering systems, and in particular, cell culture chambers that provide increased cell yield. A cell culture chamber may include a first body forming a first portion of the cell culture chamber. A cell culture chamber may include a second body forming a second portion of the cell culture chamber, the first body and the second body configured to couple together, forming an enclosed volume, wherein a first non-porous, gas-permeable material is disposed on the first body; and wherein the first non-porous, gas-permeable material and the first body are formed together. A second non-porous, gas-permeable material may be disposed on the second body, and the second non-porous, gas-permeable material and the second body may be formed together. The cell culture chamber may further include at least one bubble trap integrally formed with the first body and/or the second body.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/04 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
The present disclosure provides cell culture chambers for use in automated cell engineering systems, and in particular, cell culture chambers that provide increased cell yield. A cell culture chamber may include a first body forming a first portion of the cell culture chamber. A cell culture chamber may include a second body forming a second portion of the cell culture chamber, the first body and the second body configured to couple together, forming an enclosed volume, wherein a first non-porous, gas-permeable material is disposed on the first body; and wherein the first non-porous, gas-permeable material and the first body are formed together. A second non-porous, gas-permeable material may be disposed on the second body, and the second non-porous, gas-permeable material and the second body may be formed together. The cell culture chamber may further include at least one bubble trap integrally formed with the first body and/or the second body.
The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12N 5/0783 - Cellules TCellules NKProgéniteurs de cellules T ou NK
18.
STERILE SAMPLING METHODS AND DEVICES FOR AUTOMATED CELL ENGINEERING SYSTEMS
Devices and methods for sterile sampling from automated cell engineering systems are provided. Sterile sampling devices are configured to maintain sterility of a sample reservoir during intake and expulsion of fluids or other material to the sterile sampling devices. Methods provided herein employ sterile sampling devices to achieve the sterile withdrawal and sterile injection of materials and fluids to and from an automated cell engineering system.
The present disclosure provides devices and associated methods for temperature monitoring and control in automated biological material engineering systems, including cell engineering systems. The devices and methods utilize measurement of internal temperatures in an automated system to map temperatures during the various processes carried out in the systems.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
20.
Cell concentration methods and devices for use in automated bioreactors
The present disclosure provides cassettes for use in automated cell engineering systems that include cell concentration filters for reducing fluid volume of a cell sample during or following automated processing. The disclosure also provides methods of concentrating a cell population, as well as automated cell engineering systems that can utilize the cassettes and carry out the methods.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
An automated carousel and system configured for translationally moving a plurality of biological production units in unison along a vertical frame while maintaining a precise alignment of each of the biological production units relative to gravity, and simultaneously providing independent dynamic adjustment of the axial orientation of each of the plurality of biological production units relative to gravity. The automated carousel may be adapted for use with a variety of biological production units supporting cell culture and/or tissue engineering systems in various clinical and laboratory settings and provides for ergonomic use thereof.
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
The present technology is generally related to storage structures for cell engineering systems. The storage structures allow for multiple automated cell engineering systems to be held via a single structure, and presented to a user when desired or needed for direct access to the cell engineering system, and then returned to a storage or working position. Many storage structures can be arranged together in a clinical or hospital setting, or other cell therapy engineering manufacturing environment.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
23.
SUPPORT STRUCTURES FOR AUTOMATED CELL ENGINEERING SYSTEMS
The present technology is generally related to storage structures for cell engineering systems. The storage structures allow for multiple automated cell engineering systems to be held via a single structure, and presented to a user when desired or needed for direct access to the cell engineering system, and then returned to a storage or working position. Many storage structures can be arranged together in a clinical or hospital setting, or other cell therapy engineering manufacturing environment.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
The present technology is generally related to storage structures for cell engineering systems. The storage structures allow for multiple automated cell engineering systems to be held via a single structure, and presented to a user when desired or needed for direct access to the cell engineering system, and then returned to a storage or working position. Many storage structures can be arranged together in a clinical or hospital setting, or other cell therapy engineering manufacturing environment.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
25.
Cell culture and tissue engineering systems with controlled environmental zones
An automated cell culture and tissue engineering system comprising defined and separate environmental zones provide for increased control and maintenance of the internal environment of the system such that the temperature, air flow and gases surrounding the bioreactor module form one zone that is maintained separately to a second zone formed surrounding the reagent fluid reservoir. The system further comprises means for elimination and/or management of condensation within the second zone of the system.
C12M 1/02 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'agitationAppareillage pour l'enzymologie ou la microbiologie avec des moyens d'échange de chaleur
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
The present disclosure provides an automated method of producing viral vectors, utilizing engineered viral vector-producing cell lines, or packaging cells, within a fully-enclosed cell engineering system. Exemplary viral vectors that can be produced include lentivirus vectors, adeno-associated virus vectors, baculovirus vectors and retrovirus vectors.
The present disclosure provides combinatorial fluid switches that allow for the selection of a single fluid flow-path, while controlling multiple flow-paths. The combinatorial fluid switches can be used in various biological systems and processes, included automated cell engineering systems.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 3/06 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus avec des moyens de filtration, d'ultrafiltration, d'osmose inverse ou de dialyse
F16K 11/20 - Soupapes ou clapets à voies multiples, p. ex. clapets mélangeursRaccords de tuyauteries comportant de tels clapets ou soupapesAménagement d'obturateurs et de voies d'écoulement spécialement conçu pour mélanger les fluides dont plusieurs éléments de fermeture ne se déplacent pas comme un tout actionnés par des organes de commande distincts
F16K 99/00 - Matière non prévue dans les autres groupes de la présente sous-classe
28.
COMBINATORIAL FLUID SWITCH FOR USE IN AUTOMATED CELL ENGINEERING SYSTEMS
The present disclosure provides combinatorial fluid switches that allow for the selection of a single fluid flow-path, while controlling multiple flow-paths. The combinatorial fluid switches can be used in various biological systems and processes, included automated cell engineering systems.
C12M 3/06 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus avec des moyens de filtration, d'ultrafiltration, d'osmose inverse ou de dialyse
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
F16K 11/20 - Soupapes ou clapets à voies multiples, p. ex. clapets mélangeursRaccords de tuyauteries comportant de tels clapets ou soupapesAménagement d'obturateurs et de voies d'écoulement spécialement conçu pour mélanger les fluides dont plusieurs éléments de fermeture ne se déplacent pas comme un tout actionnés par des organes de commande distincts
F16K 99/00 - Matière non prévue dans les autres groupes de la présente sous-classe
29.
PROCESS CONTROL SYSTEMS FOR AUTOMATED CELL ENGINEERING SYSTEMS
Systems and methods for process control of automated cell engineering systems are provided. Automated cell engineering systems provide automated cell processing functionality. Automated process control systems provide control, interconnectivity, monitoring, data archival, software updating, and other oversight functions for automated cell engineering systems. Further, central control process systems provide control, monitoring, data archival, software updating, and other oversight functions for automated process control systems.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
G16H 10/40 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données relatives aux analyses de laboratoire, p. ex. pour des analyses d’échantillon de patient
The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.
C07K 14/705 - RécepteursAntigènes de surface cellulaireDéterminants de surface cellulaire
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.
C07K 14/705 - RécepteursAntigènes de surface cellulaireDéterminants de surface cellulaire
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.
C07K 14/705 - RécepteursAntigènes de surface cellulaireDéterminants de surface cellulaire
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
Devices and methods for sterile sampling from automated cell engineering systems are provided. Sterile sampling devices are configured to maintain sterility of a sample reservoir during intake and expulsion of fluids or other material to the sterile sampling devices. Methods provided herein employ sterile sampling devices to achieve the sterile withdrawal and sterile injection of materials and fluids to and from an automated cell engineering system.
A61M 1/00 - Dispositifs de succion ou de pompage à usage médicalDispositifs pour retirer, traiter ou transporter les liquides du corpsSystèmes de drainage
A61M 3/00 - Seringues médicales, p. ex. clystèresIrrigateurs
A61M 5/00 - Dispositifs pour faire pénétrer des agents dans le corps par introduction sous-cutanée, intravasculaire ou intramusculaireAccessoires à cet effet, p. ex. dispositifs de remplissage ou de nettoyage, appuis-bras
34.
TEMPERATURE CALIBRATION METHODS AND DEVICES FOR USE IN AUTOMATED BIOREACTORS
The present disclosure provides devices and associated methods for temperature monitoring and control in automated biological material engineering systems, including cell engineering systems. The devices and methods utilize measurement of internal temperatures in an automated system to map temperatures during the various processes carried out in the systems.
The present disclosure provides devices and associated methods for temperature monitoring and control in automated biological material engineering systems, including cell engineering systems. The devices and methods utilize measurement of internal temperatures in an automated system to map temperatures during the various processes carried out in the systems.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
Devices and methods for sterile sampling from automated cell engineering systems are provided. Sterile sampling devices are configured to maintain sterility of a sample reservoir during intake and expulsion of fluids or other material to the sterile sampling devices. Methods provided herein employ sterile sampling devices to achieve the sterile withdrawal and sterile injection of materials and fluids to and from an automated cell engineering system.
A61M 5/00 - Dispositifs pour faire pénétrer des agents dans le corps par introduction sous-cutanée, intravasculaire ou intramusculaireAccessoires à cet effet, p. ex. dispositifs de remplissage ou de nettoyage, appuis-bras
A61M 3/00 - Seringues médicales, p. ex. clystèresIrrigateurs
A61M 1/00 - Dispositifs de succion ou de pompage à usage médicalDispositifs pour retirer, traiter ou transporter les liquides du corpsSystèmes de drainage
Systems, devices and methods for automatic magnetic separation of magnetized targets in a biological sample are herein disclosed, where they comprise a magnetic field shield/barrier controllably operable to control the magnetic field in terms of reaching and attracting the magnetized targets within the biological sample.
B03C 1/02 - Séparation magnétique agissant directement sur la substance à séparer
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12N 5/0783 - Cellules TCellules NKProgéniteurs de cellules T ou NK
38.
Quality control methods for automated cell processing
The present disclosure provides methods for assessing and optimizing cellular quality of a cell-based therapy that is being produced in an automated cell engineering system. The methods suitably include monitoring molecular characteristics of the cells before, during, and after the automated process to provide feedback to the process parameters. In embodiments, the cells being produced are Chimeric Antigen Receptor (CAR) T-cells.
The present disclosure provides cell culture chambers for use in automated cell engineering systems, and in particular, cell culture chambers that include improved cell-contacting surfaces. Improved cell-contacting surfaces can include a surface coating that promotes cell growth, adherence, differentiation, maintenance of phenotype, and/or improves transduction; a cell-contacting surface comprising a non-porous, gas-permeable material; as well as other modifications to the cell-contacting surfaces. Cassettes comprising the cell culture chambers are also provided.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/02 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'agitationAppareillage pour l'enzymologie ou la microbiologie avec des moyens d'échange de chaleur
C12M 1/04 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
40.
CELL CULTURE CHAMBER WITH IMPROVED CELL-CONTACTING SURFACES
The present disclosure provides cell culture chambers for use in automated cell engineering systems, and in particular, cell culture chambers that include improved cell-contacting surfaces. Improved cell-contacting surfaces can include a surface coating that promotes cell growth, adherence, differentiation, maintenance of phenotype, and/or improves transduction; a cell-contacting surface comprising a non-porous, gas-permeable material; as well as other modifications to the cell-contacting surfaces. Cassettes comprising the cell culture chambers are also provided.
The present disclosure provides cell culture chambers for use in automated cell engineering systems, and in particular, cell culture chambers that include improved cell-contacting surfaces. Improved cell-contacting surfaces can include a surface coating that promotes cell growth, adherence, differentiation, maintenance of phenotype, and/or improves transduction; a cell-contacting surface comprising a non-porous, gas-permeable material; as well as other modifications to the cell-contacting surfaces. Cassettes comprising the cell culture chambers are also provided.
Systems and methods for process control of automated cell engineering systems are provided. Automated cell engineering systems provide automated cell processing functionality. Automated process control systems provide control, interconnectivity, monitoring, data archival, software updating, and other oversight functions for automated cell engineering systems. Further, central control process systems provide control, monitoring, data archival, software updating, and other oversight functions for automated process control systems.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G16H 40/63 - TIC spécialement adaptées à la gestion ou à l’administration de ressources ou d’établissements de santéTIC spécialement adaptées à la gestion ou au fonctionnement d’équipement ou de dispositifs médicaux pour le fonctionnement d’équipement ou de dispositifs médicaux pour le fonctionnement local
G16H 40/67 - TIC spécialement adaptées à la gestion ou à l’administration de ressources ou d’établissements de santéTIC spécialement adaptées à la gestion ou au fonctionnement d’équipement ou de dispositifs médicaux pour le fonctionnement d’équipement ou de dispositifs médicaux pour le fonctionnement à distance
09 - Appareils et instruments scientifiques et électriques
Produits et services
Powered mounting racks specially adapted for holding and
housing pharmaceutical manufacturing machines that are used
for autologous cell therapies comprising a non-disposable
enclosed bioreactor for cell culturing with disposable
units. Mounting racks specially adapted for housing laboratory use
multiple cell therapy micro-bioreactors and also featuring
power supplies and power connectors for use in connection
therewith.
The invention provides systems, modules, bioreactor and methods for the automated culture, proliferation, differentiation, production and maintenance of tissue engineered products. In one aspect is an automated tissue engineering system comprising a housing, at least one bioreactor supported by the housing, the bioreactor facilitating physiological cellular functions and/or the generation of one or more tissue constructs from cell and/or tissue sources. A fluid containment system is supported by the housing and is in fluid communication with the bioreactor. One or more sensors are associated with one or more of the housing, bioreactor or fluid containment system for monitoring parameters related to the physiological cellular functions and/or generation of tissue constructs; and a microprocessor linked to one or more of the sensors. The systems, methods and products of the invention find use in various clinical and laboratory settings.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
09 - Appareils et instruments scientifiques et électriques
Produits et services
(1) Mounting racks specially adapted for housing laboratory and therapeutic use multiple cell therapy micro-bioreactors, and also featuring power supplies and power connectors for use in connection therewith; Electrical or battery-powered mounting racks specially adapted for holding and housing pharmaceutical manufacturing machines that are used for autologous cell therapies comprising a non-disposable enclosed bioreactor for cell culturing with disposable units
46.
Cell concentration methods and devices for use in automated bioreactors
The present disclosure provides cassettes for use in automated cell engineering systems that include cell concentration filters for reducing fluid volume of a cell sample during or following automated processing. The disclosure also provides methods of concentrating a cell population, as well as automated cell engineering systems that can utilize the cassettes and carry out the methods.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
An automated cell culture and tissue engineering system comprising defined and separate environmental zones provide for increased control and maintenance of the internal environment of the system such that the temperature, air flow and gases surrounding the bioreactor module form one zone that is maintained separately to a second zone formed surrounding the reagent fluid reservoir. The system further comprises means for elimination and/or management of condensation within the second zone of the system.
C12M 1/02 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'agitationAppareillage pour l'enzymologie ou la microbiologie avec des moyens d'échange de chaleur
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
48.
CELL CULTURE AND TISSUE ENGINEERING SYSTEMS WITH CONTROLLED ENVIRONMENTAL ZONES
An automated cell culture and tissue engineering system comprising defined and separate environmental zones provide for increased control and maintenance of the internal environment of the system such that the temperature, air flow and gases surrounding the bioreactor module form one zone that is maintained separately to a second zone formed surrounding the reagent fluid reservoir. The system further comprises means for elimination and/or management of condensation within the second zone of the system.
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
49.
CELL CULTURE AND TISSUE ENGINEERING SYSTEMS WITH CONTROLLED ENVIRONMENTAL ZONES
An automated cell culture and tissue engineering system comprising defined and separate environmental zones provide for increased control and maintenance of the internal environment of the system such that the temperature, air flow and gases surrounding the bioreactor module form one zone that is maintained separately to a second zone formed surrounding the reagent fluid reservoir. The system further comprises means for elimination and/or management of condensation within the second zone of the system.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
09 - Appareils et instruments scientifiques et électriques
Produits et services
(1) Powered mounting racks specially adapted for holding and housing pharmaceutical manufacturing machines that are used for autologous cell therapies comprising a non-disposable enclosed bioreactor for cell culturing with disposable units.
(2) Mounting racks specially adapted for housing laboratory use multiple cell therapy micro-bioreactors and also featuring power supplies and power connectors for use in connection therewith.
09 - Appareils et instruments scientifiques et électriques
Produits et services
Powered mounting racks specially adapted for holding and housing pharmaceutical manufacturing machines that are used for autologous cell therapies comprising a non-disposable enclosed bioreactor for cell culturing with disposable units Mounting racks specially adapted for housing laboratory use multiple cell therapy micro-bioreactors, and also featuring power supplies and power connectors for use in connection therewith
An automated carousel and system configured for translationally moving a plurality of biological production units in unison along a vertical frame while maintaining a precise alignment of each of the biological production units relative to gravity, and simultaneously providing independent dynamic adjustment of the axial orientation of each of the plurality of biological production units relative to gravity. The automated carousel may be adapted for use with a variety of biological production units supporting cell culture and/or tissue engineering systems in various clinical and laboratory settings and provides for ergonomic use thereof.
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
An automated carousel and system configured for translationally moving a plurality of biological production units in unison along a vertical frame while maintaining a precise alignment of each of the biological production units relative to gravity, and simultaneously providing independent dynamic adjustment of the axial orientation of each of the plurality of biological production units relative to gravity. The automated carousel may be adapted for use with a variety of biological production units supporting cell culture and/or tissue engineering systems in various clinical and laboratory settings and provides for ergonomic use thereof.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12Q 3/00 - Procédés de commande sensible aux conditions du milieu
An automated carousel and system configured for translationally moving a plurality of biological production units in unison along a vertical frame while maintaining a precise alignment of each of the biological production units relative to gravity, and simultaneously providing independent dynamic adjustment of the axial orientation of each of the plurality of biological production units relative to gravity. The automated carousel may be adapted for use with a variety of biological production units supporting cell culture and/or tissue engineering systems in various clinical and laboratory settings and provides for ergonomic use thereof.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12Q 3/00 - Procédés de commande sensible aux conditions du milieu
55.
BEDSIDE AUTOMATED CELL ENGINEERING SYSTEM AND METHODS
The present disclosure provides cell therapy production systems that can suitably be used in a patient bedside setting. Such systems allow for direct removal of a patient's blood, automated processing to produce a cell therapy, and then infusion back into the patient, without the need to remove the system from the patient's bedside. Also provided herein are systems for production of cell therapies in a bedside setting.
Systems, devices and methods for automatic magnetic separation of magnetized targets in a biological sample are herein disclosed, where they comprise a magnetic field shield/barrier controllably operable to control the magnetic field in terms of reaching and attracting the magnetized targets within the biological sample.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
C12N 5/0783 - Cellules TCellules NKProgéniteurs de cellules T ou NK
C12Q 1/00 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions
C12Q 1/24 - Méthodes d'échantillonnage, d'inoculation ou de développement d'un échantillonMéthodes pour isoler physiquement un micro-organisme intact
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
Systems, devices and methods for automatic magnetic separation of magnetized targets in a biological sample are herein disclosed, where they comprise a magnetic field shield/barrier controllably operable to control the magnetic field in terms of reaching and attracting the magnetized targets within the biological sample.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
C12N 5/0783 - Cellules TCellules NKProgéniteurs de cellules T ou NK
C12Q 1/00 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.
C07K 14/705 - RécepteursAntigènes de surface cellulaireDéterminants de surface cellulaire
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.
The invention provides systems, modules, bioreactor and methods for the automated culture, proliferation, differentiation, production and maintenance of tissue engineered products. In one aspect is an automated tissue engineering system comprising a housing, at least one bioreactor supported by the housing, the bioreactor facilitating physiological cellular functions and/or the generation of one or more tissue constructs from cell and/or tissue sources. A fluid containment system is supported by the housing and is in fluid communication with the bioreactor. One or more sensors are associated with one or more of the housing, bioreactor or fluid containment system for monitoring parameters related to the physiological cellular functions and/or generation of tissue constructs; and a microprocessor linked to one or more of the sensors. The systems, methods and products of the invention find use in various clinical and laboratory settings.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
The invention provides systems, modules, bioreactor and methods for the automated culture, proliferation, differentiation, production and maintenance of tissue engineered products. In one aspect is an automated tissue engineering system comprising a housing, at least one bioreactor supported by the housing, the bioreactor facilitating physiological cellular functions and/or the generation of one or more tissue constructs from cell and/or tissue sources. A fluid containment system is supported by the housing and is in fluid communication with the bioreactor. One or more sensors are associated with one or more of the housing, bioreactor or fluid containment system for monitoring parameters related to the physiological cellular functions and/or generation of tissue constructs; and a microprocessor linked to one or more of the sensors. The systems, methods and products of the invention find use in various clinical and laboratory settings.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
The invention provides systems, modules, bioreactor and methods for the automated culture, proliferation, differentiation, production and maintenance of tissue engineered products. In one aspect is an automated tissue engineering system comprising a housing, at least one bioreactor supported by the housing, the bioreactor facilitating physiological cellular functions and/or the generation of one or more tissue constructs from cell and/or tissue sources. A fluid containment system is supported by the housing and is in fluid communication with the bioreactor. One or more sensors a associated with one or more of the housing, bioreactor or fluid containment system for monitoring parameters related to the physiological cellular functions and/or generation of tissue constructs; and a microprocessor linked to one or more of the sensors. The systems, methods and products of the invention find use in various clinical and laboratory settings.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
The automated cell culture arrangement according to the invention comprises at least one closed cell culture module with at least one bioreactor. The closed cell culture module is a closed system, which means that within the closed cell culture module a closed sterile environment can be maintained. The automated cell culture arrangement according to the invention, further comprises at least one pump for pumping liquids within the closed cell culture module and at least one additional tool module, which is configured or configurable to act upon or to monitor the contents of a bioreactor and is movable relative to the at least one closed cell culture module or it is movable relative to one or several components of the at least one closed cell culture module.
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
The automated cell culture arrangement according to the invention comprises at least one closed cell culture module with at least one bioreactor. The closed cell culture module is a closed system, which means that within the closed cell culture module a closed sterile environment can be maintained. The automated cell culture arrangement according to the invention, further comprises at least one pump for pumping liquids within the closed cell culture module and at least one additional tool module which is configured or configurable to act upon or to monitor the contents of a bioreactor and is movable relative to the at
The invention is a reverse-flow method and system for the loading, proliferation and differentiation of cells into and throughout an implantable biocompatible three-dimensional scaffold.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
The invention is an automated advanced tissue engineering system that comprises a housing in which one or more tissue engineering modules are accommodated together with a central microprocessor that controls functioning of the tissue engineering modules. In one embodiment, the tissue engineering module comprises a housing supporting one or more bioreactor chamber assemblies and a fluid reservoir operationally engageable with the housing. The bioreactor chamber assemblies may be selected depending on the end product option desired and may include, for example, a cell therapy bioreactor chamber, a single implant bioreactor chamber and a multiple (mosaic) implant bioreactor chamber.
The invention provides systems, modules, bioreactor and methods for the automated culture, proliferation, differentiation, production and maintenance of tissue engineered products. In one aspect is an automated tissue engineering system comprising a housing, at least one bioreactor supported by the housing, the bioreactor facilitating physiological cellular functions and/or the generation of one or more tissue constructs from cell and/or tissue sources. A fluid containment system is supported by the housing and is in fluid communication with the bioreactor. One or more sensors are associated with one or more of the housing, bioreactor or fluid containment system for monitoring parameters related to the physiological cellular functions and/or generation of tissue constructs; and a microprocessor linked to one or more of the sensors. The systems, methods and products of the invention find use in various clinical and laboratory settings.
The invention is an automated advanced tissue engineering system that comprises a housing in which one or more tissue engineering modules are accomodated together with a central microprocessor that controls functioning of the tissue engineering modules. In one embodiment, the tissue engineering module comprises a housing supporting one or more bioreactor chamber assemblies and a fluid reservoir operationally engageable with the housing. The bioreactor chamber assemblies may be selected depending on the end product option desired and may include, for example, a cell therapy bioreactor chamber, a single implant bioreactor chamber and a multiple (mosaic) implant bioreactor chamber.
