Abstract

The invention relates to a microplate (10) for microscopy of an organoid (30), comprising: a body (11) having at least one recess (20), the recess (20) being adapted to contain and restrict movement of the organoid (30), and a reflective surface (40), the reflective surface (40) being inclined in relation to the recess (20), such that an incoming beam of light towards the microplate (10) is directed onto the organoid (30) in a substantially horizontal direction.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 21/64 - FluorescencePhosphorescence
• G01N 21/03 - Cuvette constructions
• G06T 7/00 - Image analysis

Goods & Services

Microscopes; cytometers; microscope video cameras; microscope video processors; incubators for laboratory use; downloadable software for cell imaging for use in microscopes; cytometers, microscope video cameras, microscope video processors and incubators for laboratory use

Goods & Services

Microscopes; cytometers; microscope video cameras; microscope video processors; incubators for laboratoty use; software for use in microscopes, cytometers; microscope video cameras, microscope video processors and incubators for laboratoty use; fluorescence analyzers; holographic apparatus and imaging; measuring instruments.

Abstract

The invention relates to a microplate (10) for microscopy of an organoid (30), comprising: a body (11) having at least one recess (20), the recess (20) being adapted to contain and restrict movement of the organoid (30), and a reflective surface (40), the reflective surface (40) being inclined in relation to the recess (20), such that an incoming beam of light towards the microplate (10) is directed onto the organoid (30) in a substantially horizontal direction.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 21/64 - FluorescencePhosphorescence
• C12M 1/32 - Inoculator or sampler multiple field or continuous type
• G03H 1/02 - Holographic processes or apparatus using light, infrared, or ultraviolet waves for obtaining holograms or for obtaining an image from themDetails peculiar thereto Details

Abstract

The present invention relates to a molecularly imprinted polymer for binding glycans, wherein the molecularly imprinted polymer is obtainable by providing a saccharide template such as a glycan; providing at least two functional monomers capable of cooperatively; interacting with the template; providing a crosslinking monomer; polymerizing the monomers optionally dissolved in a solvent, in presence of the saccharide template; and removing the template from the formed polymer. The invention is also related to a method for their production and use of the molecularly imprinted polymer.

IPC Classes  ?

• C08F 251/00 - Macromolecular compounds obtained by polymerising monomers on to polysaccharides or derivatives thereof
• A61K 47/36 - PolysaccharidesDerivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
• A61K 31/726 - Glycosaminoglycans, i.e. mucopolysaccharides
• B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
• B01J 20/26 - Synthetic macromolecular compounds
• B01J 20/285 - Porous sorbents based on polymers
• C08F 292/00 - Macromolecular compounds obtained by polymerising monomers on to inorganic materials
• B01J 20/30 - Processes for preparing, regenerating or reactivating
• C08F 220/00 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride, ester, amide, imide, or nitrile thereof

Abstract

The present invention relates to a molecularly imprinted polymer for binding glycans, wherein the molecularly imprinted polymer is obtainable by providing a saccharide template such as a glycan; providing at least two functional monomers capable of cooperatively; interacting with the template; providing a crosslinking monomer; polymerizing the monomers optionally dissolved in a solvent, in presence of the saccharide template; and removing the template from the formed polymer. The invention is also related to a method for their production and use of the molecularly imprinted polymer.

IPC Classes  ?

• B01J 20/26 - Synthetic macromolecular compounds
• A61K 31/00 - Medicinal preparations containing organic active ingredients
• B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
• B01J 20/285 - Porous sorbents based on polymers
• C08F 220/00 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride, ester, amide, imide, or nitrile thereof
• C08F 292/00 - Macromolecular compounds obtained by polymerising monomers on to inorganic materials
• C08F 293/00 - Macromolecular compounds obtained by polymerisation on to a macromolecule having groups capable of inducing the formation of new polymer chains bound exclusively at one or both ends of the starting macromolecule

Goods & Services

Recorded data files containing test results from microscopes and cytometers; software for calculation and transformation of data from microscopes and cytometers into images, numeric test results, calculating tables and diagrams; holographic images. Diagrams and calculating tables. Evaluation of test results from microscopes and cytometers.

Goods & Services

Recorded data files containing test results from microscopes and cytometers; software for calculation and transformation of data from microscopes and cytometers into images, numeric test results, calculating tables and diagrams; holographic images. Diagrams and calculating tables. Evaluation of test results from microscopes and cytometers.

Goods & Services

Microscopes; cytometers; microscope video cameras; microscope video processors; incubators for laboratory use; software for use in microscopes; cytometers, microscope video cameras, microscope video processors and incubators for laboratory use.

Abstract

A method based on digital holography for determining parameters of at least one erythrocyte is provided, said method comprising the steps of: constructing an interference pattern comprising a wave front representative of said at least one erythrocyte arised from the interaction of said at least one erythrocyte and electromagnetic radiation, reconstructing amplitude-and phase information representative of said at least one erythrocyte wave front from said interference pattern and determining the mean corpuscular volume and/or mean corpuscular hemoglobin concentration and/or oxygen saturation and/or mean corpuscular hemoglobin of at least one erythrocyte by combining phase information and amplitude information representative of said at least one erythrocyte.

IPC Classes  ?

• G01B 9/021 - Interferometers using holographic techniques
• G01N 21/41 - RefractivityPhase-affecting properties, e.g. optical path length
• G03H 1/08 - Synthesising holograms
• G01N 21/45 - RefractivityPhase-affecting properties, e.g. optical path length using interferometric methodsRefractivityPhase-affecting properties, e.g. optical path length using Schlieren methods
• G03H 1/04 - Processes or apparatus for producing holograms

Goods & Services

Microscopes; cytometers; microscope video cameras; microscope video processors; incubators for laboratoty use; software for use in microscopes; cytometers, microscope video cameras, microscope video processors and incubators for laboratoty use.

Abstract

The present invention relates to use of a digital holographic microscopy and imaging setup and a method of digital holographic microscopy and imaging for detecting molecules or structures stained or labelled to at least one cell or conjugated to antibodies which are bound either directly to said at least one cell or indirectly via another or several antibodies in a chain bound to said at least one cell.

IPC Classes  ?

• G03H 1/00 - Holographic processes or apparatus using light, infrared, or ultraviolet waves for obtaining holograms or for obtaining an image from themDetails peculiar thereto
• G03H 1/04 - Processes or apparatus for producing holograms
• G02B 21/00 - Microscopes
• C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving viable microorganisms
• G03H 1/08 - Synthesising holograms

Abstract

A method based on digital holography for determining parameters of at least one erythrocyte is provided, said method comprising the steps of: constructing an interference pattern comprising a wave front representative of said at least one erythrocyte arised from the interaction of said at least one erythrocyte and electromagnetic radiation, reconstructing amplitude-and phase information representative of said at least one erythrocyte wave front from said interference pattern and determining the mean corpuscular volume and/or mean corpuscular hemoglobin concentration and/or oxygen saturation and/or mean corpuscular hemoglobin of at least one erythrocyte by combining phase information and amplitude information representative of said at least one erythrocyte.

IPC Classes  ?

• G03H 1/08 - Synthesising holograms
• G01B 9/021 - Interferometers using holographic techniques
• G01N 21/45 - RefractivityPhase-affecting properties, e.g. optical path length using interferometric methodsRefractivityPhase-affecting properties, e.g. optical path length using Schlieren methods

Abstract

This invention relates to digital holographic microscopy for analyzing a sample comprising at least one translucent biological object. A disclosed apparatus comprises at least one light source arranged to create at least one beam of coherent light, the at least one beam comprising at least one object beam, a sensor, a beam directing means arranged to direct said at least one beam, and a sample holder adapted to hold the sample. The sample holder comprises a first reflecting surface which in use is arranged below the sample. The first reflecting surface is essentially flat and is arranged to reflect the at least one object beam. The light source, the beam directing means and the sample holder are arranged relatively each other such that the at least one object beam is directed towards the first reflecting surface at an angle which is essentially perpendicular to the first reflecting surface, and such that the at least one object beam that has passed through the sample is directed towards the sensor. The disclosed invention may be used to achieve a better signal-to-noise ratio when analyzing translucent biological objects by means of digital holography.

IPC Classes  ?

• G03H 1/00 - Holographic processes or apparatus using light, infrared, or ultraviolet waves for obtaining holograms or for obtaining an image from themDetails peculiar thereto
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G02B 21/00 - Microscopes

Abstract

The present invention relates to use of a digital holographic microscopy and imaging setup and a method of digital holographic microscopy and imaging for detecting molecules or structures stained or labelled to at least one cell or conjugated to antibodies which are bound either directly to said at least one cell or indirectly via another or several antibodies in a chain bound to said at least one cell.

IPC Classes  ?

• G03H 1/04 - Processes or apparatus for producing holograms
• G01N 21/21 - Polarisation-affecting properties
• G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
• G01N 21/64 - FluorescencePhosphorescence
• G02B 21/00 - Microscopes
• G01B 9/04 - Measuring microscopes

Abstract

Method for analyzing a sample comprising at least one ovum or embryo, the method being based on digital holographic imaging, the method comprising the following steps: creating at least one object beam and at least one reference beam of light, where said at least one object beam and said at least one reference beam are mutually coherent; exposing said sample to said at least one object beam; superimposing said at least one object beam that has passed through said sample with said at least one reference beam and thereby creating an interference pattern; detecting said interference pattern, called hologram; reconstructing phase and/or amplitude information of object wavefront from said interference pattern; and constructing at least one ovum or embryo analysis image and determining at least one ovum or embryo quality showing parameter from said phase and/or amplitude information.

IPC Classes  ?

• G01N 21/45 - RefractivityPhase-affecting properties, e.g. optical path length using interferometric methodsRefractivityPhase-affecting properties, e.g. optical path length using Schlieren methods
• G01B 9/021 - Interferometers using holographic techniques
• G03H 1/08 - Synthesising holograms

Abstract

A non-destructive method and device for analyzing a sample comprising transparent living and/or dead cells, by 5 means of a digital holographic microscope, where the sample (8) is exposed to light from a laser (2). The light that travels through the cells in the sample will experience a difference in the optical path length compared to the surrounding media and the wave front that emerges from the 10 cells will thus be phase shifted. This distortion can be detected in the digital hologram, which is reconstructed from the interference pattern detected by a digital sensor (17), such as a CCD or a CMOS, as phase differences or phase shifts and thereby creating a digital hologram. The 15 phase shift of each element of the hologram is then used for analyzing the characteristics of the cells in the sample.

IPC Classes  ?

• G01B 9/021 - Interferometers using holographic techniques

Abstract

The present invention relates to an observation vessel for digital holo- graphic microscopy of at least one transparent biological object, an observation vessel lid for digital holographic microscopy of at least one transparent biological object as well as a method for analyzing a sample comprising at least one transparent biological object and at least one medium by means of digital holographic microscopy.

IPC Classes  ?

• G03H 1/02 - Holographic processes or apparatus using light, infrared, or ultraviolet waves for obtaining holograms or for obtaining an image from themDetails peculiar thereto Details
• G01L 3/00 - Measuring torque, work, mechanical power, or mechanical efficiency, in general
• G01B 9/021 - Interferometers using holographic techniques

Abstract

A non-destructive method and device for analyzing a sample comprising transparent living and/or dead cells, by 5 means of a digital holographic microscope, where the sample (8) is exposed to light from a laser (2). The light that travels through the cells in the sample will experience a difference in the optical path length compared to the surrounding media and the wave front that emerges from the 10 cells will thus be phase shifted. This distortion can be detected in the digital hologram, which is reconstructed from the interference pattern detected by a digital sensor (17), such as a CCD or a CMOS, as phase differences or phase shifts and thereby creating a digital hologram. The 15 phase shift of each element of the hologram is then used for analyzing the characteristics of the cells in the sample.

IPC Classes  ?

• G03H 1/08 - Synthesising holograms
• G01B 9/021 - Interferometers using holographic techniques
• G02B 21/18 - Arrangements with more than one light-path, e.g. for comparing two specimens