The invention relates to a microplate (10) for microscopy of an organoid (30), comprising: a body (11) having at least one recess (20), the recess (20) being adapted to contain and restrict movement of the organoid (30), and a reflective surface (40), the reflective surface (40) being inclined in relation to the recess (20), such that an incoming beam of light towards the microplate (10) is directed onto the organoid (30) in a substantially horizontal direction.
09 - Appareils et instruments scientifiques et électriques
Produits et services
Microscopes; cytometers; microscope video cameras; microscope video processors; incubators for laboratory use; downloadable software for cell imaging for use in microscopes; cytometers, microscope video cameras, microscope video processors and incubators for laboratory use
09 - Appareils et instruments scientifiques et électriques
Produits et services
Microscopes; cytometers; microscope video cameras; microscope video processors; incubators for laboratoty use; software for use in microscopes, cytometers; microscope video cameras, microscope video processors and incubators for laboratoty use; fluorescence analyzers; holographic apparatus and imaging; measuring instruments.
The invention relates to a microplate (10) for microscopy of an organoid (30), comprising: a body (11) having at least one recess (20), the recess (20) being adapted to contain and restrict movement of the organoid (30), and a reflective surface (40), the reflective surface (40) being inclined in relation to the recess (20), such that an incoming beam of light towards the microplate (10) is directed onto the organoid (30) in a substantially horizontal direction.
C12M 1/32 - Inoculateur ou échantillonneur du type à champs multiples ou en continu
G03H 1/02 - Procédés ou appareils holographiques utilisant la lumière, les infrarouges ou les ultraviolets pour obtenir des hologrammes ou pour en obtenir une imageLeurs détails spécifiques Détails
Bundesrepublik Deutschland, vertreten durch den Bundesminister für Wirtschaft und Energie (Allemagne)
Inventeur(s)
Shinde, Sudhirkumar
Wingren, Anette Gjörloff
Rurack, Knut
Sellergren, Börje
Wan, Wei
Abrégé
The present invention relates to a molecularly imprinted polymer for binding glycans, wherein the molecularly imprinted polymer is obtainable by providing a saccharide template such as a glycan; providing at least two functional monomers capable of cooperatively; interacting with the template; providing a crosslinking monomer; polymerizing the monomers optionally dissolved in a solvent, in presence of the saccharide template; and removing the template from the formed polymer. The invention is also related to a method for their production and use of the molecularly imprinted polymer.
B01D 15/38 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p. ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
B01J 20/285 - Absorbants ou adsorbants poreux à base de polymères
C08F 292/00 - Composés macromoléculaires obtenus par polymérisation de monomères sur des substances inorganiques
B01J 20/30 - Procédés de préparation, de régénération ou de réactivation
C08F 220/00 - Copolymères de composés contenant un ou plusieurs radicaux aliphatiques non saturés, chaque radical ne contenant qu'une seule liaison double carbone-carbone et un seul étant terminé par un seul radical carboxyle ou un sel, anhydride, ester, amide, imide ou nitrile
BUNDESREPUBLIK DEUTSCHLAND, VERTRETEN DURCH DEN BUNDESMINISTER FÜR WIRTSCHAFT UND ENERGIE, DIESER VERTRETEN DURCH DEN PRÄSIDENTEN DER BUNDESANSTALT FÜR MATERIALFORSCHUNG - UND PRÜFUNG (Allemagne)
PHASE HOLOGRAPHIC IMAGING PHI AB (Suède)
Inventeur(s)
Sellergren, Börje
Shinde, Sudhirkumar
Gjörloff Wingren, Anette
Rurack, Knut
Wan, Wei
Abrégé
The present invention relates to a molecularly imprinted polymer for binding glycans, wherein the molecularly imprinted polymer is obtainable by providing a saccharide template such as a glycan; providing at least two functional monomers capable of cooperatively; interacting with the template; providing a crosslinking monomer; polymerizing the monomers optionally dissolved in a solvent, in presence of the saccharide template; and removing the template from the formed polymer. The invention is also related to a method for their production and use of the molecularly imprinted polymer.
A61K 31/00 - Préparations médicinales contenant des ingrédients actifs organiques
B01D 15/38 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction spécifique non couverte par un ou plusieurs des groupes , p. ex. chromatographie d'affinité, chromatographie d'échange par ligand ou chromatographie chirale
B01J 20/285 - Absorbants ou adsorbants poreux à base de polymères
C08F 220/00 - Copolymères de composés contenant un ou plusieurs radicaux aliphatiques non saturés, chaque radical ne contenant qu'une seule liaison double carbone-carbone et un seul étant terminé par un seul radical carboxyle ou un sel, anhydride, ester, amide, imide ou nitrile
C08F 292/00 - Composés macromoléculaires obtenus par polymérisation de monomères sur des substances inorganiques
C08F 293/00 - Composés macromoléculaires obtenus par polymérisation sur une macromolécule contenant des groupes capables d'amorcer la formation de nouvelles chaînes polymères rattachées exclusivement à une ou aux deux extrémités de la macromolécule de départ
09 - Appareils et instruments scientifiques et électriques
16 - Papier, carton et produits en ces matières
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Recorded data files containing test results from microscopes
and cytometers; software for calculation and transformation
of data from microscopes and cytometers into images, numeric
test results, calculating tables and diagrams; holographic
images. Diagrams and calculating tables. Evaluation of test results from microscopes and cytometers.
09 - Appareils et instruments scientifiques et électriques
16 - Papier, carton et produits en ces matières
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Recorded data files containing test results from microscopes and cytometers; software for calculation and transformation of data from microscopes and cytometers into images, numeric test results, calculating tables and diagrams; holographic images. Diagrams and calculating tables. Evaluation of test results from microscopes and cytometers.
09 - Appareils et instruments scientifiques et électriques
Produits et services
Microscopes; cytometers; microscope video cameras;
microscope video processors; incubators for laboratory use;
software for use in microscopes; cytometers, microscope
video cameras, microscope video processors and incubators
for laboratory use.
10.
Determination of physical parameters associated with erythrocytes
A method based on digital holography for determining parameters of at least one erythrocyte is provided, said method comprising the steps of: constructing an interference pattern comprising a wave front representative of said at least one erythrocyte arised from the interaction of said at least one erythrocyte and electromagnetic radiation, reconstructing amplitude-and phase information representative of said at least one erythrocyte wave front from said interference pattern and determining the mean corpuscular volume and/or mean corpuscular hemoglobin concentration and/or oxygen saturation and/or mean corpuscular hemoglobin of at least one erythrocyte by combining phase information and amplitude information representative of said at least one erythrocyte.
G01B 9/021 - Interféromètres utilisant des techniques holographiques
G01N 21/41 - RéfringencePropriétés liées à la phase, p. ex. longueur du chemin optique
G03H 1/08 - Procédés ou appareils pour produire des hologrammes pour faire des hologrammes synthétiques
G01N 21/45 - RéfringencePropriétés liées à la phase, p. ex. longueur du chemin optique en utilisant des méthodes interférométriquesRéfringencePropriétés liées à la phase, p. ex. longueur du chemin optique en utilisant les méthodes de Schlieren
G03H 1/04 - Procédés ou appareils pour produire des hologrammes
09 - Appareils et instruments scientifiques et électriques
Produits et services
Microscopes; cytometers; microscope video cameras; microscope video processors; incubators for laboratoty use; software for use in microscopes; cytometers, microscope video cameras, microscope video processors and incubators for laboratoty use.
12.
Method for and use of digital holographic microscopy and imaging on labelled cell samples
The present invention relates to use of a digital holographic microscopy and imaging setup and a method of digital holographic microscopy and imaging for detecting molecules or structures stained or labelled to at least one cell or conjugated to antibodies which are bound either directly to said at least one cell or indirectly via another or several antibodies in a chain bound to said at least one cell.
G03H 1/00 - Procédés ou appareils holographiques utilisant la lumière, les infrarouges ou les ultraviolets pour obtenir des hologrammes ou pour en obtenir une imageLeurs détails spécifiques
G03H 1/04 - Procédés ou appareils pour produire des hologrammes
C12Q 1/02 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des micro-organismes viables
G03H 1/08 - Procédés ou appareils pour produire des hologrammes pour faire des hologrammes synthétiques
13.
DETERMINATION OF PHYSICAL PARAMETERS ASSOCIATED WITH ERYTHROCYTES
A method based on digital holography for determining parameters of at least one erythrocyte is provided, said method comprising the steps of: constructing an interference pattern comprising a wave front representative of said at least one erythrocyte arised from the interaction of said at least one erythrocyte and electromagnetic radiation, reconstructing amplitude-and phase information representative of said at least one erythrocyte wave front from said interference pattern and determining the mean corpuscular volume and/or mean corpuscular hemoglobin concentration and/or oxygen saturation and/or mean corpuscular hemoglobin of at least one erythrocyte by combining phase information and amplitude information representative of said at least one erythrocyte.
G03H 1/08 - Procédés ou appareils pour produire des hologrammes pour faire des hologrammes synthétiques
G01B 9/021 - Interféromètres utilisant des techniques holographiques
G01N 21/45 - RéfringencePropriétés liées à la phase, p. ex. longueur du chemin optique en utilisant des méthodes interférométriquesRéfringencePropriétés liées à la phase, p. ex. longueur du chemin optique en utilisant les méthodes de Schlieren
14.
DIGITAL HOLOGRAPHIC MICROSCOPY OF TRANSLUCENT BIOLOGICAL OBJECTS
This invention relates to digital holographic microscopy for analyzing a sample comprising at least one translucent biological object. A disclosed apparatus comprises at least one light source arranged to create at least one beam of coherent light, the at least one beam comprising at least one object beam, a sensor, a beam directing means arranged to direct said at least one beam, and a sample holder adapted to hold the sample. The sample holder comprises a first reflecting surface which in use is arranged below the sample. The first reflecting surface is essentially flat and is arranged to reflect the at least one object beam. The light source, the beam directing means and the sample holder are arranged relatively each other such that the at least one object beam is directed towards the first reflecting surface at an angle which is essentially perpendicular to the first reflecting surface, and such that the at least one object beam that has passed through the sample is directed towards the sensor. The disclosed invention may be used to achieve a better signal-to-noise ratio when analyzing translucent biological objects by means of digital holography.
G03H 1/00 - Procédés ou appareils holographiques utilisant la lumière, les infrarouges ou les ultraviolets pour obtenir des hologrammes ou pour en obtenir une imageLeurs détails spécifiques
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
The present invention relates to use of a digital holographic microscopy and imaging setup and a method of digital holographic microscopy and imaging for detecting molecules or structures stained or labelled to at least one cell or conjugated to antibodies which are bound either directly to said at least one cell or indirectly via another or several antibodies in a chain bound to said at least one cell.
G01N 21/31 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique
Method for analyzing a sample comprising at least one ovum or embryo, the method being based on digital holographic imaging, the method comprising the following steps: creating at least one object beam and at least one reference beam of light, where said at least one object beam and said at least one reference beam are mutually coherent; exposing said sample to said at least one object beam; superimposing said at least one object beam that has passed through said sample with said at least one reference beam and thereby creating an interference pattern; detecting said interference pattern, called hologram; reconstructing phase and/or amplitude information of object wavefront from said interference pattern; and constructing at least one ovum or embryo analysis image and determining at least one ovum or embryo quality showing parameter from said phase and/or amplitude information.
G01N 21/45 - RéfringencePropriétés liées à la phase, p. ex. longueur du chemin optique en utilisant des méthodes interférométriquesRéfringencePropriétés liées à la phase, p. ex. longueur du chemin optique en utilisant les méthodes de Schlieren
G01B 9/021 - Interféromètres utilisant des techniques holographiques
G03H 1/08 - Procédés ou appareils pour produire des hologrammes pour faire des hologrammes synthétiques
17.
Method and apparatus for analysis of a sample of cells
A non-destructive method and device for analyzing a sample comprising transparent living and/or dead cells, by 5 means of a digital holographic microscope, where the sample (8) is exposed to light from a laser (2). The light that travels through the cells in the sample will experience a difference in the optical path length compared to the surrounding media and the wave front that emerges from the 10 cells will thus be phase shifted. This distortion can be detected in the digital hologram, which is reconstructed from the interference pattern detected by a digital sensor (17), such as a CCD or a CMOS, as phase differences or phase shifts and thereby creating a digital hologram. The 15 phase shift of each element of the hologram is then used for analyzing the characteristics of the cells in the sample.
The present invention relates to an observation vessel for digital holo- graphic microscopy of at least one transparent biological object, an observation vessel lid for digital holographic microscopy of at least one transparent biological object as well as a method for analyzing a sample comprising at least one transparent biological object and at least one medium by means of digital holographic microscopy.
G03H 1/02 - Procédés ou appareils holographiques utilisant la lumière, les infrarouges ou les ultraviolets pour obtenir des hologrammes ou pour en obtenir une imageLeurs détails spécifiques Détails
G01L 3/00 - Mesure du couple, du travail, de la puissance ou du rendement mécanique en général
G01B 9/021 - Interféromètres utilisant des techniques holographiques
19.
METHOD AND APPARATUS FOR ANALYSIS OF A SAMPLE OF CELLS
A non-destructive method and device for analyzing a sample comprising transparent living and/or dead cells, by 5 means of a digital holographic microscope, where the sample (8) is exposed to light from a laser (2). The light that travels through the cells in the sample will experience a difference in the optical path length compared to the surrounding media and the wave front that emerges from the 10 cells will thus be phase shifted. This distortion can be detected in the digital hologram, which is reconstructed from the interference pattern detected by a digital sensor (17), such as a CCD or a CMOS, as phase differences or phase shifts and thereby creating a digital hologram. The 15 phase shift of each element of the hologram is then used for analyzing the characteristics of the cells in the sample.
