Abstract

A Pichia pastoris strain with high-temperature resistance and a high methanol conversion rate. By mean of using a protoplast fusion method, the strain with a high methanol utilization rate, Pichia pastoris HTX-33 (CGMCC NO. 25207) is fused with a high-temperature-resistant Pichia manshurica strain screened from rotten wood from the vineyards of Yuanshi Vineyard in Yinchuan, Ningxia Province to obtain a hybrid strain that is resistant to high temperatures and efficiently utilizes methanol. The hybrid strain is identified as a Pichia pastoris strain and can achieve high biomass growth and high methanol utilization at 37°C by means of using methanol as the sole carbon source. The strain has the advantages of being green, environmentally-friendly, pollution-free, high in expression rate, low in cost, etc.

IPC Classes  ?

• C12N 1/16 - YeastsCulture media therefor
• A23K 10/16 - Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions

Abstract

Provided a genetically modified yeast strain for producing succinic acid, which strain has the activity or an enhanced activity of an NADPH-dependent malate dehydrogenase (EC 1.1.1.82), and optionally also has the activity or an enhanced activity of at least one of the following: (i) soluble fumarate reductase (EC 4.2.1.2), (ii) a pyruvate carboxylase (EC 6.4.1.1), (iii) a fumarase (EC 4.2.1.2), and (iv) succinate transport protein; and a preparation method therefor, a method for producing succinic acid using same, and the use thereof.

IPC Classes  ?

• C12P 7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
• C07K 14/39 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from fungi from yeasts
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12N 9/88 - Lyases (4.)

Abstract

The present invention relates to a fusion protein comprising a nucleic acid binding protein and a method for capturing a specific nucleic acid using same. The fusion protein is formed by fusing a cytosine base editor and a uracil binding protein. The method is a method for capturing, from a sample, DNA (containing a PAM and containing cytosine C at a specific position) containing a specific target sequence, and comprises: contacting a sample containing target DNA with the fusion protein to capture the target DNA, and detecting the target DNA. Under the mediation of a sgRNA library, provided is a use of the fusion protein in capturing, from a sample, DNA containing a specific target sequence and/or detecting the DNA. Target DNA can be captured from a complex nucleic acid sample with high throughput and high sensitivity and specificity, and practical application value is provided.

IPC Classes  ?

• C07K 19/00 - Hybrid peptides
• C12N 9/24 - Hydrolases (3.) acting on glycosyl compounds (3.2)
• C12N 9/78 - Hydrolases (3.) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/62 - DNA sequences coding for fusion proteins
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

The present invention provides a genetically modified malic acid producing yeast strain, wherein the strain has or has enhanced malate transport protein activity and has or has enhanced NADPH-dependent malate dehydrogenase (EC 1.1.1.82) activity, optionally also has or has enhanced at least one of the following activities: (i) pyruvate carboxylase (EC 6.4.1.1) activity, (ii) phosphoenolpyruvate carboxykinase (EC 4.1.1.49) activity, (iii) phosphoenolpyruvate carboxylase activity, and (iv) biotin transport protein activity; and a preparation method thereof, a method for producing L-malic acid using the same, and use thereof.

IPC Classes  ?

• C12P 7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
• C07K 14/39 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from fungi from yeasts
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12N 9/88 - Lyases (4.)

Abstract

A new light-controlled repressor protein OptoLacl and a light-controlled gene expression system constructed on the basis of OptoLacl.

IPC Classes  ?

• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/75 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
• C12R 1/19 - Escherichia coli
• C12R 1/125 - Bacillus subtilis
• C12R 1/07 - Bacillus

Abstract

Provided are a gRNA mutant and the use thereof, and further provided are a method for constructing same and a base editor containing same. The gRNA mutant is used in the base editor, and can universally reduce a base editing window, thereby improving the specificity of gene editing, and achieving specific editing on one base.

IPC Classes  ?

• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseasesGene therapy
• C12N 9/22 - Ribonucleases
• C12N 9/78 - Hydrolases (3.) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

Abstract

Provided are a method for preparing starch using carbon dioxide, a recombinant microorganism, a method for constructing the recombinant microorganism, and a reagent. The method for preparing starch using carbon dioxide comprises: (1) providing energy and carbon sources for microbial cells on the basis of carbon dioxide and extracellular non-optical energy; and (2) generating starch within the microbial cells on the basis of at least one of up-regulated glucose-1-phosphate adenylyltransferase and starch synthase in the microbial cells. In this way, by utilizing non-optical energy, such as electric energy or hydrogen energy, starch can be effectively prepared inside the microbial cells by fixing carbon dioxide.

IPC Classes  ?

• C12P 19/04 - Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• C12N 9/10 - Transferases (2.)
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)

Abstract

Provided in the present invention are a genetically modified pantoic-acid-producing strain having, or having enhanced, NADH-dependent acetohydroxy acid isomeroreductase, a method for producing same, a method for producing D-pantoic acid by using same, and the use thereof in the production of D-pantoic acid.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12P 7/42 - Hydroxy carboxylic acids
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12R 1/19 - Escherichia coli

Abstract

The present invention provides a bispecific antibody for a broad-spectrum novel coronavirus. The bispecific antibody of the present invention comprises a first targeting domain D1, which targets a first epitope of a SARS-CoV-2RBD domain; and a second targeting domain D2, which targets a second epitope of the SARS-CoV-2RBD domain. The bispecific antibody of the present invention has broad-spectrum neutralizing activity, can effectively neutralize SARS‐CoV‐2 and a variety of SARS‐CoV‐2 novel coronavirus variants having strong infectivity and high harmfulness, and therefore has great application value in prevention, treatment and/or detection of novel coronavirus infection.

IPC Classes  ?

• C07K 16/46 - Hybrid immunoglobulins
• C07K 16/10 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
• C12N 15/13 - Immunoglobulins
• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
• A61K 39/42 - AntibodiesImmunoglobulinsImmune serum, e.g. antilymphocytic serum viral
• A61P 31/14 - Antivirals for RNA viruses
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

The present application provides a monoclonal antibody or an antigen-binding fragment thereof that specifically binds to the RBD domain of SARS-CoV or SARS-CoV-2, a preparation method therefor, and the use thereof. The monoclonal antibody or the antigen-binding fragment thereof can bind to the RBD antigen of SARS-CoV or SARS-CoV-2 with a high affinity, and can neutralize SARS-CoV and SARS-CoV-2 prototype strains and a series of SARS-CoV and SARS-CoV-2 mutant strains with a relatively high neutralization activity, thereby inhibiting the infections caused by the above strains. Therefore, the monoclonal antibody or the antigen-binding fragment thereof has great potential application value in clinical treatment, prevention and/or detection of infections caused by SARS-CoV and SARS-CoV-2 prototype strains and SARS-CoV and SARS-CoV-2 mutant strains.

IPC Classes  ?

• C07K 16/10 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
• C12N 15/13 - Immunoglobulins
• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
• A61K 39/42 - AntibodiesImmunoglobulinsImmune serum, e.g. antilymphocytic serum viral
• A61P 31/14 - Antivirals for RNA viruses
• G01N 33/577 - ImmunoassayBiospecific binding assayMaterials therefor involving monoclonal antibodies
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

A polynucleotide having promoter activity of a malate dehydrogenase gene (mdh gene), a transcription expression cassette, recombinant expression vector and recombinant host cell containing the polynucleotide having the promoter activity, a method for constructing a promoter mutant, a method for regulating the transcription of a target gene, a method for preparing a protein, and method for producing a target compound. The polynucleotide having the promoter activity is a mutant of an mdh gene promoter, and compared with a promoter of a wild-type mdh gene, the promoter activity of the mutant is significantly improved. After operably linking the mutant to a target gene, the expression efficiency of the target gene can be significantly improved, thereby effectively improving the yield and transformation rate of a target compound.

IPC Classes  ?

• C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12N 15/67 - General methods for enhancing the expression
• C12P 13/04 - Alpha- or beta-amino acids

Abstract

A protein having an L-proline efflux function and the use thereof are provided. A method for producing L-proline or hydroxyproline by means of using a protein ThrE is used for producing L-proline by means of enhancing the activity of a polypeptide, having an L-proline efflux function, in an L-proline-producing strain. Alternatively, the method is used for producing hydroxyproline by means of weakening the activity of a polypeptide, having an L-proline efflux function, in L-proline-producing host cells and enhancing the activity of a proline hydroxylase.

IPC Classes  ?

• C12P 13/24 - ProlineHydroxyprolineHistidine
• C07K 14/34 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria from Corynebacterium (G)
• C12N 1/20 - BacteriaCulture media therefor
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase

Abstract

Provided are a microorganism for producing a pantoic acid, and a construction method therefor and an application thereof. The microorganism for producing the pantoic acid is obtained by knocking out a gene in Escherichia coli and introducing an exogenous gene. The obtained microorganism is Escherichia coli that is registered in the China General Microbiological Culture Collection Center with an accession number of CGMCC No. 21699. A pantoic acid synthesis pathway has been opened up, and accumulation of the pantoic acid can be achieved in a fermentation process.

IPC Classes  ?

• C12P 7/42 - Hydroxy carboxylic acids
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12N 9/10 - Transferases (2.)
• C12N 9/88 - Lyases (4.)
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/52 - Genes encoding for enzymes or proenzymes

Abstract

Provided is a recombinant yeast expressing germacrene A synthetase or a fusion protein thereof, wherein the fusion protein is germacrene A synthetase and farnesyl pyrophosphate synthase. The recombinant yeast improves the yield of germacrene A, and is suitable for the industrialized production of β-elemene and/or germacrene A.

IPC Classes  ?

• C12N 1/18 - Baker's yeastBrewer's yeast
• C12N 9/88 - Lyases (4.)
• C12N 9/10 - Transferases (2.)
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12P 5/00 - Preparation of hydrocarbons

Abstract

Provided is a method for biosynthesis of a 1,4-dihydroxy-2-butanone compound, using benzaldehyde lyase as a catalyst to catalyze a hydroxymethylation reaction of 3-hydroxypropanal and formaldehyde to generate 1,4-dihydroxy-2-butanone. Cheap and easily available aldehydes are used as a substrate to synthesize 1,4-dihydroxy-2-butanone, providing a green and sustainable method which has application value.

IPC Classes  ?

• C12P 7/26 - Ketones

Abstract

Provided are polypeptides with tagatose 6-phosphate epimerase activity, polypeptides with tagatose 6-phosphate phosphatase activity, a composition comprising the polypeptides with tagatose 6-phosphate epimerase activity and the polypeptides with tagatose 6-phosphate phosphatase activity, a method for preparing tagatose from the composition, and use of the composition in the preparation of tagatose.

IPC Classes  ?

• C12N 9/90 - Isomerases (5.)
• C12P 19/24 - Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose

Abstract

The present invention provides an mreC mutant and use thereof in L-valine fermentative production. The mreC mutant is a protein obtained by mutating proline at position 150 in the wild-type mreC amino acid sequence into leucine. The introduction of an mreC point mutation into the microbial genome, i.e., a C-to-T mutation at position 449 of the coding region in the wild-type mreC gene nucleotide sequence, can significantly improve the production capacity and biomass of an engineering strain of a high valine production. The knock-out and introduction of related enzyme genes of the L-valine anaerobic fermentation pathway further provide proper carbon metabolic flows and reducing capacity balance control in the L-valine production process. By the coordinative regulation of multiple genes, a metabolic pathway suitable for L-valine fermentative production is formed, thus achieving a high-level synthesis of L-valine in microorganisms.

IPC Classes  ?

• C07K 14/245 - Escherichia (G)
• C12N 15/31 - Genes encoding microbial proteins, e.g. enterotoxins
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12P 13/08 - LysineDiaminopimelic acidThreonineValine

Abstract

Provided are a polynucleotide having promoter activity and an application of the polynucleotide in producing an amino acid. Also disclosed are a transcription expression cassette, a recombinant expression vector, and a recombinant host cell which contain the polynucleotide, and a method for enhancing expression of a target gene, a method for preparing a protein, and a method for producing an amino acid. The polynucleotide having the promoter activity is a mutant of a polynucleotide having the sequence as shown in SEQ ID NO: 9. Compared with the polynucleotide having the sequence as shown in SEQ ID NO: 9, the promoter activity of the mutant is significantly enhanced, thereby promoting the stable and efficient expression of the target gene.

IPC Classes  ?

• C12P 13/08 - LysineDiaminopimelic acidThreonineValine
• C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
• C12N 9/06 - Oxidoreductases (1.), e.g. luciferase acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)

Abstract

Provided are a polypeptide with aspartate kinase activity and the use thereof in the production of an amino acid. Specifically, provided are a novel polypeptide with aspartate kinase activity, a recombinant polypeptide, a polynucleotide, a nucleic acid construct, a recombinant expression vector, a recombinant host cell, and a method for producing an amino acid. The polypeptide with aspartate kinase activity is a mutant mutated at one or more positions corresponding to positions 293, 294 and 307 of the amino acid sequence as shown in SEQ ID NO: 1. Compared with a polypeptide having the sequence as shown in SEQ ID NO: 1, the mutant polypeptide removes the feedback inhibition of lysine on aspartate kinase, has high aspartate kinase activity, and can be used for the stable and efficient production of lysine and derivatives thereof.

IPC Classes  ?

• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12P 13/08 - LysineDiaminopimelic acidThreonineValine

Abstract

A starch biosynthesis method may implement total artificial biosynthesis from simple compounds such as dihydroxyacetone, formaldehyde, formic acid and methanol to starch. By coupling with methods such as chemical reduction of carbon dioxide, even total artificial biosynthesis of starch taking carbon dioxide as a starting raw material can be implemented. The method can utilize carbon dioxide of high concentration and high density and electric energy and hydrogen energy of high energy density, is more suitable for an industrial production mode, and the production cycle is shortened from several months in farming to several days.

IPC Classes  ?

• C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)

Abstract

The present invention belongs to the technical field of crystal products, and relates to high-purity crystalline D-tagatose, a composition comprising same, a method for preparing same, and use thereof. Specifically, the crystalline D-tagatose of the present invention has a purity of 98% or greater, a particle size of 150 μm or greater, an aspect ratio of 1.0-4.0, an angle of repose of 40° or less, and a bulk density of 0.7 g/mL or greater. Compared with commercially available products, the crystalline D-tagatose has more excellent product morphology, better fluidity, and insusceptibility to agglomeration, which is conducive to reducing storage and transportation costs. In addition, the method for preparing the crystalline D-tagatose of the present invention can freely regulate the particle size of the product, eliminating the need for costly devices and facilitating the industrial production of the crystalline D-tagatose.

IPC Classes  ?

• C07H 3/02 - Monosaccharides
• C07H 1/06 - SeparationPurification

Abstract

Provided are a mutant of a Corynebacterium glutamicum glutamate dehydrogenase gene promoter and applications thereof. The mutant has improved promoter activity compared to a wild-type promoter. Hence, it can be used to enhance the expression of a target gene, for example, operably ligating the mutant with a glutamate dehydrogenase gene, and the expression intensity of the glutamate dehydrogenase can be enhanced, thereby improving the amino acid production efficiency of a recombinant strain.

IPC Classes  ?

• C12N 9/06 - Oxidoreductases (1.), e.g. luciferase acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
• C12P 13/24 - ProlineHydroxyprolineHistidine
• C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium

Abstract

Disclosed are a mutant of a pyruvate carboxylase gene promoter of Corynebacterium glutamicum and applications thereof. The mutant has improved promoter activity compared with a wild-type promoter, and can be used for enhancing expression of a target gene, for example, operably ligating the mutant to a pyruvate carboxylase gene enhances the expression intensity of the pyruvate carboxylase, thereby improving the production efficiency of amino acids of the strain.

IPC Classes  ?

• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12P 13/08 - LysineDiaminopimelic acidThreonineValine
• C12P 13/24 - ProlineHydroxyprolineHistidine
• C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium

Abstract

Disclosed are a variety of terminal deoxynucleotidyl transferases (TdTs) and variants thereof for de novo synthesis of a polynucleotide having a controlled sequence and for efficient and controllable synthesis of a nucleic acid molecule without dependence on a template. It is found that some amino acid residues of the TdT catalytic domain can be specifically modified so as to improve the ability of such modified TdT to synthesize a polynucleotide.

IPC Classes  ?

• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Abstract

Disclosed is a genetically modified yeast strain for producing malic acid. The strain has the activity or an enhanced activity of a malic acid transporter and has the activity or an enhanced activity of an NADPH-dependent malate dehydrogenase (EC 1.1.1.82); and also has the activity or an enhanced activity of at least one of the following: (i) a pyruvate carboxylase (EC 6.4.11); (ii) a phosphoenolpyruvate carboxykinase (EC 41.1.49); (iii) a phosphoenolpyruvate carboxylase; and (iv) a biotin transporter. Disclosed are a preparation method for the strain, a method for producing L-malic acid by means of using the strain, and the use thereof.

IPC Classes  ?

• C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
• C12N 1/16 - YeastsCulture media therefor
• C12P 7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid

Abstract

Provided are a genetically modified yeast strain for producing succinic acid, which strain has the activity or an enhanced activity of an NADPH-dependent malate dehydrogenase (EC1.1.1.82), and optionally also has the activity or an enhanced activity of at least one of the following: (i) a soluble fumarate reductase (EC 4.2.1.2), (ii) a pyruvate carboxylase (EC 6.4.1.1), (iii) a fumarase (EC 4.2.1.2), and (iv) a succinic acid transporter; and a preparation method therefor, a method for producing succinic acid by using same, and the use thereof.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12P 7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
• C12R 1/85 - Saccharomyces

Abstract

Provided are a recombinant microorganism constructed based on a lysine efflux protein, a method for constructing the recombinant microorganism, a lysine producing strain, and a method for producing lysine. The polypeptide having the sequence as shown in SEQ ID NO: 4 or SEQ ID NO: 6 can promote the extracellular discharge of lysine, and two novel lysine efflux proteins are provided for the transformation of a lysine high-producing strain. A recombinant microorganism is constructed based on the polypeptide having the sequence as shown in SEQ ID NO: 4 or SEQ ID NO: 6. The recombinant microorganism has significantly increased lysine yield and sugar-acid conversion rate, and is suitable for large-scale industrial production of lysine.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12P 13/08 - LysineDiaminopimelic acidThreonineValine
• C07K 14/195 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria
• C07K 14/21 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria from Pseudomonadaceae (F)
• C12R 1/15 - Corynebacterium
• C12R 1/19 - Escherichia coli

Abstract

Provided are a method for preparing starch using carbon dioxide, a recombinant microorganism, a method for constructing the recombinant microorganism, and a reagent. The method for preparing starch using carbon dioxide comprises: (1) providing energy and carbon sources for microbial cells on the basis of carbon dioxide and extracellular non-optical energy; and (2) generating starch within the microbial cells on the basis of at least one of up-regulated glucose-1-phosphate adenylyltransferase and starch synthase in the microbial cells. In this way, by utilizing non-optical energy, such as electric energy or hydrogen energy, starch can be effectively prepared inside the microbial cells by fixing carbon dioxide.

IPC Classes  ?

• C12P 19/04 - Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 1/32 - Processes using, or culture media containing, lower alkanols, i.e. C1 to C6

Abstract

Related are a recombinant microorganism for producing L-valine, a construction method and an application thereof. Through enhancing amino acid dehydrogenase activity of L-valine fermentation strain, and/or activating an Entner-Doudoroff (ED) metabolic pathway, a problem in L-valine fermentation process that reducing power is unbalanced is solved, thereby the titer and yield of L-valine produced by Escherichia coli are improved, and L-valine was produced by one-step anaerobic fermentation.

IPC Classes  ?

• C12P 13/08 - LysineDiaminopimelic acidThreonineValine
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12N 1/20 - BacteriaCulture media therefor
• C12N 9/06 - Oxidoreductases (1.), e.g. luciferase acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)

Abstract

Related are a recombinant microorganism for producing L-valine, a construction method and an application thereof. Through transferring an amino acid dehydrogenase gene and/or activating activity of a transhydrogenase and/or a NAD kinase, reducing power of NADPH in cell is increased, the titer and yield of L-valine generated by Escherichia coli are improved, and the production of L-valine by one-step anaerobic fermentation is achieved.

IPC Classes  ?

• C12P 13/08 - LysineDiaminopimelic acidThreonineValine
• C12N 9/06 - Oxidoreductases (1.), e.g. luciferase acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12N 15/90 - Stable introduction of foreign DNA into chromosome
• C12N 1/20 - BacteriaCulture media therefor
• C12N 9/88 - Lyases (4.)
• C12N 9/10 - Transferases (2.)

Abstract

Related are a recombinant microorganism for producing L-valine, a construction method and an application thereof. Through transferring an acetohydroxy acid reductoisomerase gene and/or an amino acid dehydrogenase gene into a microorganism, and enhancing activity of an acetohydroxy acid reductoisomerase and/or an amino acid dehydrogenase, the titer and yield of L-valine generated by Escherichia coli may be improved, and L-valine was produced by one-step anaerobic fermentation.

IPC Classes  ?

• C12P 13/08 - LysineDiaminopimelic acidThreonineValine
• C12N 9/06 - Oxidoreductases (1.), e.g. luciferase acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12N 15/90 - Stable introduction of foreign DNA into chromosome

Abstract

The invention discloses a construction method of genetic engineering fungi of filamentous fungi. Through the genetic engineering method, the filamentous fungi overexpress the positive regulation genes of ethanol synthesis, and/or down regulate the negative regulation genes of endogenous ethanol synthesis to obtain genetic engineering strains. Or overexpression of acetaldehyde dehydrogenase and ethanol dehydrogenase containing mitochondrial localization signal sequence, or overexpression of pyruvate decarboxylase and ethanol dehydrogenase containing mitochondrial localization signal sequence, or overexpression of acetaldehyde dehydrogenase, ethanol dehydrogenase and pyruvate decarboxylase containing mitochondrial localization signal sequence in filamentous fungal cells. Compared with the original strain, the ethanol synthesis ability of the obtained genetically engineered strains are improved.

IPC Classes  ?

• C12N 15/80 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 1/14 - Fungi Culture media therefor

Abstract

Provided are a gRNA mutant and the use thereof, and further provided are a method for constructing same and a base editor containing same. The gRNA mutant is used in the base editor, and can universally reduce a base editing window, thereby improving the specificity of gene editing, and achieving specific editing on one base.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides

Abstract

According to the copolymer film and the enzyme catalysis self-assembly synthesis method therefor provided by the present invention, the copolymer film is prepared at a two-liquid-phase interface by means of the enzyme catalysis of a film forming monomer comprising a phenolic hydroxyl group, a film forming monomer comprising at least two amino groups, and a catalyst. In the present invention, operation steps are simple, reaction conditions are mild, the selection range of the film forming monomer is wide, and a film forming monomer can be selected according to application requirements to prepare a copolymer film having a corresponding function. The preparation method of the present invention is easy to upgrade from a laboratory to industrial mass production, and has a good application prospect.

IPC Classes  ?

• C08G 83/00 - Macromolecular compounds not provided for in groups
• C08J 5/18 - Manufacture of films or sheets
• C12P 1/00 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes

Abstract

A pyruvate dehydrogenase mutant. On the basis of an amino acid sequence as shown in SEQ ID NO: 1, the 217th site of the sequence is mutated into any one of alanine, aspartic acid, glutamate, leucine and proline. The mutant can improve the yield and conversion rate of L-amino acids in a strain, growth of the strain is not inhibited while the yield is improved, and a new way is provided for large-scale production of the L-amino acids.

IPC Classes  ?

• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 15/53 - Oxidoreductases (1)
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12P 13/04 - Alpha- or beta-amino acids
• C12P 13/08 - LysineDiaminopimelic acidThreonineValine
• C12P 13/12 - MethionineCysteineCystine
• C12P 13/06 - AlanineLeucineIsoleucineSerineHomoserine
• C12R 1/19 - Escherichia coli
• C12R 1/15 - Corynebacterium

Abstract

Provided are a preparation method for an immobilized cell for tagatose production and a method for producing tagatose by using the immobilized cell. The preparation method for the immobilized cell comprises: mixing a fermentation broth of Escherichia coli or Bacillus subtilis that expresses α-glucan phosphorylase, phosphoglucomutase, glucose phosphate isomerase, tagatose 6-phosphate epimerase, and tagatose 6-phosphate phosphatase to obtain a fermentation mixture, adding inorganic soil and then performing uniform stirring, then adding a flocculant to flocculate bacteria, subsequently adding a cross-linking agent to cross-link, performing vacuum filtration to obtain a filter cake, using a rotary granulator to extrude the filter cake to granulate into a long strip, then cutting by means of a spherical shot blasting machine into particles having equal lengths, and performing boiling drying to obtain the immobilized cell for tagatose production. According to the present invention, separation and purification steps of an enzyme required in tagatose production are simplified, the recycling rate of the enzyme is improved, and the recycling of the enzyme is achieved.

IPC Classes  ?

• C12N 11/14 - Enzymes or microbial cells immobilised on or in an inorganic carrier
• C12N 11/10 - Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
• C12N 11/089 - Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• C12N 11/087 - Acrylic polymers
• C12N 11/082 - Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
• C12P 19/02 - Monosaccharides
• C12R 1/19 - Escherichia coli
• C12R 1/125 - Bacillus subtilis

Abstract

A method for preparing immobilized cells for producing mannose, and a method using same for producing mannose, comprising: fermenting to separately obtain fermentation broths of Escherichia coli or Bacillus subtilis expressing α-glucan phosphorylase, phosphoglucomutase, glucose phosphoisomerase, mannose-6-phosphate isomerase and mannose-6-phosphate phosphatase, and mixing the fermentation broths to obtain a mixed fermentation broth.

IPC Classes  ?

• C12N 11/14 - Enzymes or microbial cells immobilised on or in an inorganic carrier
• C12N 11/10 - Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
• C12N 11/089 - Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• C12N 11/087 - Acrylic polymers
• C12N 11/082 - Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
• C12P 19/02 - Monosaccharides
• C12R 1/19 - Escherichia coli
• C12R 1/125 - Bacillus subtilis

Abstract

Disclosed is a mutant of a corynebacterium glutamicum dihydrodipicolinate reductase (dapB) gene promoter. Compared with the promoter of a wild-type dapB gene, the promoter activity of the mutant is significantly improved. Also disclosed are a transcription expression cassette comprising the promoter mutant, a recombinant expression vector, a recombinant host cell, and a method for constructing a promoter mutant, a method for regulating and controlling transcription of a target gene, a method for preparing a protein, and a method for producing a target compound.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12P 13/08 - LysineDiaminopimelic acidThreonineValine
• C12R 1/01 - Bacteria or actinomycetales

Abstract

A polynucleotide having promoter activity of a malate dehydrogenase gene (mdh gene), a transcription expression cassette, recombinant expression vector and recombinant host cell containing the polynucleotide having the promoter activity, a method for constructing a promoter mutant, a method for regulating the transcription of a target gene, a method for preparing a protein, and method for producing a target compound. The polynucleotide having the promoter activity is a mutant of an mdh gene promoter, and compared with a promoter of a wild-type mdh gene, the promoter activity of the mutant is significantly improved. After operably linking the mutant to a target gene, the expression efficiency of the target gene can be significantly improved, thereby effectively improving the yield and transformation rate of a target compound.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/09 - Recombinant DNA-technology
• C12N 1/20 - BacteriaCulture media therefor
• C07K 14/34 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria from Corynebacterium (G)
• C12N 15/67 - General methods for enhancing the expression
• C12R 1/15 - Corynebacterium
• C12R 1/185 - Escherichia
• C12R 1/13 - Brevibacterium
• C12R 1/06 - Arthrobacter

Abstract

The present invention discloses base editing systems for mutating a base C to A and a base C to G and applications thereof. The base editing system for mutating C to A disclosed in the present invention includes cytosine deaminase AID and nCas9 nuclease or includes cytosine deaminase AID, nCas9 nuclease and uracil DNA glycosidase; the base editing system for mutating C to G of the present invention includes cytosine deaminase APOBEC, nCas9 nuclease and uracil DNA glycosidase. The experiments show that a combination of the three base editing systems for mutating C to A, C to T and A to G can realize a mutation of A, T, C or G to any base in both prokaryotes and eukaryotes.

IPC Classes  ?

• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12N 15/90 - Stable introduction of foreign DNA into chromosome
• C12N 9/22 - Ribonucleases
• C12N 9/78 - Hydrolases (3.) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
• C12N 9/24 - Hydrolases (3.) acting on glycosyl compounds (3.2)

Abstract

Provided are a biomimetic silicon mineralized microcapsule immobilized multi-enzyme, a preparation method therefor, and a method for producing tagatose by using same. The preparation method comprises the following steps: (1) pre-mixing glucan phosphorylase, phosphoglucomutase, phosphoglucoisomerase, 6-phosphate tagatose 4-position epimerase and 6-phosphate tagatose phosphatase solutions, then adding the mixture to a calcium chloride solution, and then pouring same into a sodium carbonate solution, stirring and separating same to obtain calcium carbonate microspheres containing a multi-enzyme; (2) mixing the calcium carbonate microspheres with a polyethyleneimine solution to obtain polyethyleneimine-calcium carbonate microspheres after separation; (3) mixing the polyethyleneimine-calcium carbonate microspheres with a silicate solution to obtain biomimetic silicon mineralized-calcium carbonate microspheres after separation; and (4) mixing the biomimetic silicon mineralized-calcium carbonate microspheres with ethylenediamine tetraacetic acid for reaction to remove calcium carbonate, and separating same to obtain a biomimetic silicon mineralized microcapsule immobilized multi-enzyme.

IPC Classes  ?

• C12N 11/18 - Multi-enzyme systems
• C12N 11/14 - Enzymes or microbial cells immobilised on or in an inorganic carrier
• C12N 11/089 - Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• C12N 11/04 - Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
• C12P 19/24 - Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
• C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
• C12P 19/16 - Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
• C12P 19/02 - Monosaccharides

Abstract

Provided are the immobilization of multiple enzymes on the basis of an artificial oil body and an application thereof in the preparation of tagatose. Specifically, an artificial oil body is used to mix an expressed fusion protein of target protease-oil body protein with an oil body, which then undergoes an ultrasonic treatment; the fusion protein is anchored to the surface of the oil body by means of the specific hydrophobicity of a human protein to form an artificial oil body containing the target protease, so that the purification and immobilization of enzymes can be completed simultaneously. The immobilized multiple enzymes that can be used for tagatose production utilize an artificial oil body as an immobilized enzyme substrate, which significantly improves the stability of the immobilized enzymes, reduces the production cost of the current enzymatic preparation of tagatose, and has a simple preparation process.

IPC Classes  ?

• C12N 11/18 - Multi-enzyme systems
• C12N 11/06 - Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
• C12P 19/24 - Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
• C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
• C12P 19/16 - Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
• C12P 19/02 - Monosaccharides

Abstract

Provided are a method for preparing an immobilized multi-enzyme system, and a method for producing tagatose by the immobilized multi-enzyme system. The immobilized multi-enzyme system is formed by uniformly mixing a porous dopamine microsphere with a multi-enzyme mixture which is used for producing tagatose. Five enzymes in an enzymatic catalysis path for converting starch to tagatose are co-immobilized by means of a porous microsphere to obtain an immobilized multi-enzyme system, the immobilized multi-enzyme system is used to catalyze conversion of starch into tagatose, and thus, enzymes can be recycled, thereby greatly reducing the amount of enzymes required for preparation of tagatose, and reducing the production cost.

IPC Classes  ?

• C12N 11/18 - Multi-enzyme systems
• C12N 11/04 - Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
• C12P 19/24 - Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
• C12P 19/02 - Monosaccharides

Abstract

Disclosed are an aspartate kinase gene expression regulatory sequence and the use thereof. By modifying positions 366-373 of a sequence represented by SEQ ID NO: 1, a polynucleotide formed by linking the sequence with a start codon GTG or TTG has transcriptional expression regulation activity, and these polynucleotides can improve expression of the aspartate kinase encoding gene lysC, such that a large amount of L-lysine is accumulated.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 15/67 - General methods for enhancing the expression
• C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
• C12P 7/44 - Polycarboxylic acids
• C12P 13/00 - Preparation of nitrogen-containing organic compounds
• C12P 13/06 - AlanineLeucineIsoleucineSerineHomoserine
• C12P 13/08 - LysineDiaminopimelic acidThreonineValine
• C12P 13/12 - MethionineCysteineCystine

Abstract

Provided are a microorganism for producing a pantoic acid, and a construction method therefor and an application thereof. The microorganism for producing the pantoic acid is obtained by knocking out a gene in Escherichia coli and introducing an exogenous gene. The obtained microorganism is Escherichia coli that is registered in the China General Microbiological Culture Collection Center with an accession number of CGMCC No. 21699. A pantoic acid synthesis pathway has been opened up, and accumulation of the pantoic acid can be achieved in a fermentation process.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/31 - Genes encoding microbial proteins, e.g. enterotoxins
• C12P 13/00 - Preparation of nitrogen-containing organic compounds
• C12R 1/19 - Escherichia coli

Abstract

Disclosed are an aspartate kinase gene expression regulatory sequence and the use thereof. By modifying positions 366-373 of a sequence represented by SEQ ID NO: 1, a polynucleotide formed by linking the sequence with a start codon GTG or TTG has transcriptional expression regulation activity, and these polynucleotides can improve expression of the aspartate kinase encoding gene lysC, such that a large amount of L-lysine is accumulated.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
• C12N 15/67 - General methods for enhancing the expression
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12P 13/08 - LysineDiaminopimelic acidThreonineValine
• C12P 13/06 - AlanineLeucineIsoleucineSerineHomoserine
• C12P 13/12 - MethionineCysteineCystine
• C12P 13/00 - Preparation of nitrogen-containing organic compounds
• C12P 7/44 - Polycarboxylic acids
• C12R 1/15 - Corynebacterium

Abstract

The present invention relates to the field of bioengineering, and specifically to genetically engineered bacillus subtilis for efficient exogenous protein expression and high-density culture. Genetic modification is performed on histidine-deficient bacillus subtilis, and the function of hisC gene is recovered by means of genetic manipulation, to obtain a non-auxotrophic strain. Further, a strain obtained by knocking out genes spoIIAC and srfAC is more suitable for expression of a variety of exogenous proteins. The non-auxotrophic bacillus subtilis for expressing exogenous proteins obtained in the present invention can provide a low-cost high-density fermentation technology, so as to greatly lower the fermentation cost and achieve high-level expression of exogenous proteins.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/54 - Transferases (2)
• C12N 9/10 - Transferases (2.)
• C12N 15/75 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
• C12R 1/125 - Bacillus subtilis

Abstract

Provided are a polynucleotide having promoter activity and use thereof in the production of target compounds. Specifically, provided are a polynucleotide having promoter activity, a transcriptional expression cassette, recombinant expression vector, recombinant host cell comprising the polynucleotide having promoter activity, a method for regulating the transcription of a target gene, a method for preparing a protein, and a method for producing target compounds. The provided polynucleotide having promoter activity is a high salt and high osmolality-inducible promoter, and has enhanced promoter activity in an environment of increased salt concentration and osmolality. The polynucleotide is operatively connected to the target gene, can significantly improve the expression intensity of the target gene in the stress environment of high salt and high osmolality, thereby stably and efficiently producing a downstream product, and effectively solving the current problems of adding expensive inducers such as IPTG and causing toxicity to a strain.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12P 13/04 - Alpha- or beta-amino acids
• C12P 7/44 - Polycarboxylic acids
• C12R 1/15 - Corynebacterium

Abstract

Disclosed in the present invention are a protein having an L-proline efflux function, and the use thereof. A method for producing L-proline or hydroxyproline by means of using a protein ThrE is used for producing L-proline by means of enhancing the activity of a polypeptide, having an L-proline efflux function, in an L-proline-producing strain. Alternatively, the method is used for producing hydroxyproline by means of weakening the activity of a polypeptide, having an L-proline efflux function, in L-proline-producing host cells and enhancing the activity of a proline hydroxylase.

IPC Classes  ?

• C12P 13/24 - ProlineHydroxyprolineHistidine
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material

Abstract

Provided are a bacillus subtilis genetically engineered bacterium for producing tagatose and a method for preparing tagatose. The genetically engineered bacterium comprises constructing thermostable α-glucan phosphorylases, thermostable glucose phosphomutases, thermostable glucose phosphate isomerases, thermostable 6-tagatose phosphate epimerases, and thermostable 6-tagatose phosphate phosphatases which are independently expressed or co-expressed. The usage of the genetically engineered bacterium can effectively convert starch into tagatose. Compared with existing methods for producing tagatose, the method has advantages such as suitability for whole-cell recycling, high safety, high yield, simple production process, low cost, and easiness in large-scale preparation.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/75 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
• C12N 11/14 - Enzymes or microbial cells immobilised on or in an inorganic carrier
• C12P 19/02 - Monosaccharides
• C12R 1/125 - Bacillus subtilis

Abstract

Disclosed is a method for producing at least one target product from glycolic acid under the action of an enzyme. In order to solve the key problem of losing 25% fixed organic carbon during the recycling process of natural glycolic acid in C3 plants, the present application designs a glycolic acid metabolic pathway comprising an acetate kinase, a phosphoacetyl transferase, a glycolyl coenzyme A reductase and an acetyl phosphate synthase; or the pathway comprises a glycolyl coenzyme A synthase, a glycolyl coenzyme A reductase, an acetyl phosphate synthase and a phosphoacetyl transferase. A new glycolic acid metabolic pathway reduces the loss of organic carbon caused by the recycling process of glycolic acid, and converts 100% of the byproduct glycolic acid generated by photosynthesis into acetyl coenzyme A, thereby providing a new idea for improving the photosynthesis of plants. The pathway also provides a method for preparing glycolaldehyde or acetyl coenzyme A by using glycolic acid as a raw material.

IPC Classes  ?

• C12P 9/00 - Preparation of organic compounds containing a metal or atom other than H, N, C, O, S, or halogen
• C12P 17/16 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing two or more hetero rings
• C12P 7/24 - Preparation of oxygen-containing organic compounds containing a carbonyl group
• C12N 9/10 - Transferases (2.)
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• A01H 6/46 - Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
• A01H 6/54 - Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
• A01H 6/82 - Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
• A01H 6/38 - Euphorbiaceae, e.g. Poinsettia

Abstract

Provided are a recombinant microorganism modified by means of genetic engineering and an application of the recombinant microorganism in production of tagatose, a preparation method for the recombinant microorganism, a tagatose production strain and a tagatose production method. According to the recombinant microorganism, the tagatose is produced by taking glucose as a substrate or taking glycerol and glucose as substrates; the efficiency of conversion and production of tagatose by means of the recombinant microorganism is high, and a multi-enzyme purification process step is not needed.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12P 19/02 - Monosaccharides
• C12R 1/19 - Escherichia coli
• C12R 1/15 - Corynebacterium
• C12R 1/125 - Bacillus subtilis
• C12R 1/225 - Lactobacillus
• C12R 1/865 - Saccharomyces cerevisiae

Abstract

Provided are a polypeptide with aspartate kinase activity and the use thereof in the production of an amino acid. Specifically, provided are a novel polypeptide with aspartate kinase activity, a recombinant polypeptide, a polynucleotide, a nucleic acid construct, a recombinant expression vector, a recombinant host cell, and a method for producing an amino acid. The polypeptide with aspartate kinase activity is a mutant mutated at one or more positions corresponding to positions 293, 294 and 307 of the amino acid sequence as shown in SEQ ID NO: 1. Compared with a polypeptide having the sequence as shown in SEQ ID NO: 1, the mutant polypeptide removes the feedback inhibition of lysine on aspartate kinase, has high aspartate kinase activity, and can be used for the stable and efficient production of lysine and derivatives thereof.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 1/20 - BacteriaCulture media therefor
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 15/54 - Transferases (2)
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12P 13/08 - LysineDiaminopimelic acidThreonineValine

Abstract

The present invention relates to a use of a degradable composite material in oil absorption. The degradable composite material comprises a three-dimensional net-shaped structure formed by fungal hyphae and at least one lignocellulose fragment fixed by the three-dimensional net-shaped structure. The degradable composite material has good oil absorption performance and has good application potential in the aspects of petroleum leakage, removal of oil stains on water, treatment of oily wastewater, and the like. Furthermore, the problem of treatment of agricultural and forestry residues such as straw and wood chips can be solved, the additional value of the residues is increased, environmental pollution is reduced, and coordinated development of economy, ecological environment, and society is facilitated.

IPC Classes  ?

• C02F 1/40 - Devices for separating or removing fatty or oily substances or similar floating material
• C02F 1/28 - Treatment of water, waste water, or sewage by sorption
• B01J 20/24 - Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
• B01J 20/30 - Processes for preparing, regenerating or reactivating
• C08L 97/02 - Lignocellulosic material, e.g. wood, straw or bagasse
• C09K 3/32 - Materials not provided for elsewhere for treating liquid pollutants, e.g. oil, gasoline or fat
• D03D 15/00 - Woven fabrics characterised by the material, structure or properties of the fibres, filaments, yarns, threads or other warp or weft elements used
• C12N 11/08 - Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer

Abstract

A use of a degradable composite material for humidity control. The degradable composite material comprises a three-dimensional network structure formed by fungal hyphae, and at least one type of lignocellulose debris fixed by the three-dimensional network structure. A composite structure of the hyphae and the lignocellulose debris is formed as the hyphae grow and entangle the lignocellulose debris. The material has good moisture absorption and moisture liberation capabilities and can be used as a humidity control material. In addition, the composite material is a natural degradable organic humidity control material, which is green and safe.

IPC Classes  ?

• C08L 97/02 - Lignocellulosic material, e.g. wood, straw or bagasse
• C08L 101/16 - Compositions of unspecified macromolecular compounds the macromolecular compounds being biodegradable
• C12N 1/14 - Fungi Culture media therefor
• C09K 3/00 - Materials not provided for elsewhere
• C12R 1/645 - Fungi

Abstract

Use of a mycelium material in oil absorption. The mycelium material is composed of hyphae of fungi. The mycelium material has relatively good oil absorption performance, and thus has good application potential in oil leakages, removal of oil pollutant on water, oily wastewater treatment, adsorption of oil mist in gas, and so on.

IPC Classes  ?

• C02F 3/34 - Biological treatment of water, waste water, or sewage characterised by the microorganisms used

Abstract

Provided is a starch biosynthesis method. The method may implement total artificial biosynthesis from simple compounds such as dihydroxyacetone, formaldehyde, formic acid and methanol to starch, and by coupling with methods such as chemical reduction of carbon dioxide, even total artificial biosynthesis of starch taking carbon dioxide as a starting raw material can be implemented. Natural starch synthesis is required to be subjected to the Calvin cycle, needing 21-22 reaction steps in total, and the present method merely needs 9-12 reaction steps. Nearly a half of the reaction steps is reduced, and a production cycle is greatly shortened. In addition, the present method can utilize carbon dioxide having high concentration and high density and electric energy and hydrogen energy having high energy density, is more suitable for an industrial production mode, and the production cycle is shortened from several months in farming to several days.

IPC Classes  ?

• C12P 19/04 - Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• C12P 19/14 - Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase, e.g. by alpha-amylase
• C12N 15/54 - Transferases (2)

Abstract

Provided are a polynucleotide having promoter activity and an application of the polynucleotide in producing an amino acid. Also disclosed are a transcription expression cassette, a recombinant expression vector, and a recombinant host cell which contain the polynucleotide, and a method for enhancing expression of a target gene, a method for preparing a protein, and a method for producing an amino acid. The polynucleotide having the promoter activity is a mutant of a polynucleotide having the sequence as shown in SEQ ID NO: 9. Compared with the polynucleotide having the sequence as shown in SEQ ID NO: 9, the promoter activity of the mutant is significantly enhanced, thereby promoting the stable and efficient expression of the target gene.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
• C12N 15/53 - Oxidoreductases (1)
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12P 13/08 - LysineDiaminopimelic acidThreonineValine
• C12R 1/15 - Corynebacterium

Abstract

Provided are a polynucleotide having promoter activity and an application of the polynucleotide in producing an amino acid. Also disclosed are a transcription expression cassette, a recombinant expression vector, and a recombinant host cell which contain the polynucleotide, and a method for enhancing expression of a target gene, a method for preparing a protein, and a method for producing an amino acid. The polynucleotide having the promoter activity is a mutant of a polynucleotide having the sequence as shown in SEQ ID NO: 9. Compared with the polynucleotide having the sequence as shown in SEQ ID NO: 9, the promoter activity of the mutant is significantly enhanced, thereby promoting the stable and efficient expression of the target gene.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/53 - Oxidoreductases (1)
• C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
• C12P 13/08 - LysineDiaminopimelic acidThreonineValine

Abstract

Provided are a mutant of a Corynebacterium glutamicum glutamate dehydrogenase gene promoter and an application thereof. The mutant has improved promoter activity compared to a wild-type promoter. Hence, same can be used to enhance the expression of a target gene, for example, operably linking same with a glutamate dehydrogenase gene, and the expression intensity of the glutamate dehydrogenase can be enhanced, thereby improving the amino acid production efficiency of a recombinant strain.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/67 - General methods for enhancing the expression
• C12P 13/04 - Alpha- or beta-amino acids
• C12P 13/14 - Glutamic acidGlutamine

Abstract

Disclosed are a mutant of a pyruvate carboxylase gene promoter of Corynebacterium glutamicum and use thereof. The mutant has improved promoter activity compared with a wild-type promoter, and can be used for enhancing expression of a target gene, for example, operably connecting the mutant to a pyruvate carboxylase gene enhances the expression intensity of the pyruvate carboxylase, thereby improving the production efficiency of amino acids of the strain.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
• C12N 15/67 - General methods for enhancing the expression
• C12P 13/04 - Alpha- or beta-amino acids
• C12R 1/15 - Corynebacterium

Abstract

Disclosed are a mutant of a pyruvate carboxylase gene promoter of Corynebacterium glutamicum and use thereof. The mutant has improved promoter activity compared with a wild-type promoter, and can be used for enhancing expression of a target gene, for example, operably connecting the mutant to a pyruvate carboxylase gene enhances the expression intensity of the pyruvate carboxylase, thereby improving the production efficiency of amino acids of the strain.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
• C12P 13/04 - Alpha- or beta-amino acids

Abstract

Provided are an allulose 3-epimerase mutant and a genetically engineered bacterium expressing the mutant. Further provided are an immobilized allulose 3-epimerase enzyme and an immobilization method thereof. A high-throughput screening method is used to obtain an allulose 3-epimerase mutant efficiently expressed in a fermentation process, which can catalyze efficient conversion of fructose to D-allulose, providing an efficient production path for key enzymes required in a D-allulose production process. Additionally, the allulose 3-epimerase is bonded to an immobilizing resin to prepare an immobilized allulose 3-epimerase enzyme. The immobilized enzyme can be applied to batch or continuous reactions to catalyze efficient conversion of fructose to D-allulose, improves the reuse rate and useful life of the enzyme, can significantly reduce the production costs of D-allulose, and can be widely applied.

IPC Classes  ?

• C12N 9/90 - Isomerases (5.)
• A23L 29/30 - Foods or foodstuffs containing additivesPreparation or treatment thereof containing carbohydrate syrupsFoods or foodstuffs containing additivesPreparation or treatment thereof containing sugarsFoods or foodstuffs containing additivesPreparation or treatment thereof containing sugar alcohols, e.g. xylitolFoods or foodstuffs containing additivesPreparation or treatment thereof containing starch hydrolysates, e.g. dextrin
• C12P 19/24 - Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose

Abstract

The present invention provides a class of new mutant proteins for increasing malic acid yield. Specifically, the present invention provides a class of new pyruvate carboxylase mutant protein and malic acid transporter mutant proteins or combinations thereof, a preparation method therefor and use thereof in improving malic acid yield.

IPC Classes  ?

• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C07K 14/37 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from fungi
• C12P 7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid

Abstract

Provided are a recombinant microorganism for producing L-valine, a construction method therefor, and an application thereof. By introducing an amino acid dehydrogenase gene and/or activating the activity of transhydrogenase and/or NAD kinase, cell NADPH reducing power is increased, the yield and conversion rate of L-valine produced by Escherichia coli are improved, and one-step anaerobic fermentation of L-valine is achieved.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 9/06 - Oxidoreductases (1.), e.g. luciferase acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/90 - Isomerases (5.)
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 15/53 - Oxidoreductases (1)
• C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
• C12P 13/08 - LysineDiaminopimelic acidThreonineValine

Abstract

Provided are a recombinant microorganism for producing L-valine, a construction method therefor and the use thereof. By means of enhancing the activity of amino acid dehydrogenase in L-valine fermentation strains, and/or activating the Entner-Doudoroff (ED) pathway, the problem of the reducing power in the fermentation process of L-valine being unbalanced is solved, thereby improving the yield and conversion rate of L-valine produced by using Escherichia coli and realizing the one-step anaerobic fermentation of L-valine.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12P 13/08 - LysineDiaminopimelic acidThreonineValine
• C12N 15/53 - Oxidoreductases (1)
• C12R 1/19 - Escherichia coli

Abstract

A recombinant microorganism for producing L-valine, a construction method therefor, and an application thereof. By introducing an acetohydroxy acid reductoisomerase gene and/or an amino acid dehydrogenase gene into a microorganism, and improving the activity of acetohydroxy acid reductoisomerase and the activity of amino acid dehydrogenase, the yield and conversion rate of L-valine produced by Escherichia coli can be increased, and one-step anaerobic fermentation of L-valine can be achieved.

IPC Classes  ?

• C12N 15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12P 13/08 - LysineDiaminopimelic acidThreonineValine
• C12R 1/13 - Brevibacterium

Abstract

A method for preparing glucosamine includes the steps of converting fructose-6-phosphate (F6P) and an ammonium salt to glucosamine-6-phosphate (GlcN6P) under the catalysis of glucosamine-6-phosphate deaminase (EC 3.5.99.6, GlmD); and producing glucosamine (GlcN) by the dephosphorylation of GlcN6P under the catalysis of an enzyme capable of catalyzing the dephosphorylation. Such a method can be used to prepare glucosamine by in vitro enzymatic biosystem.

IPC Classes  ?

• C12P 19/26 - Preparation of nitrogen-containing carbohydrates
• C12P 19/28 - N-glycosides

Abstract

Disclosed in the present invention are a formaldehyde conversion mutant protein and an application thereof. The mutant protein can catalyze preparation of 1,3-dihydroxyacetone from formaldehyde, and further synthesis of lactic acid and glycolic acid by the 1,3-dihydroxyacetone. Also disclosed is a method for synthesis of lactic acid and glycolic acid. The mutant protein in the present invention can improve the catalytic efficiency from formaldehyde to 1,3-dihydroxyacetone, and conditions are mild.

IPC Classes  ?

• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12P 7/24 - Preparation of oxygen-containing organic compounds containing a carbonyl group
• C12P 7/26 - Ketones

Abstract

Recombinant strains are obtained for the production of allulose, allose, and allitol by regulating intracellular glucose metabolism, reducing the enzyme activity of fructose 6-phosphate kinase, and enhancing the enzyme activities of glucokinase and glucose-6-phosphate isomerase, allulose 6-phosphate 3-epimerase, allulose 6-phosphate phosphatase, fructose permease and fructokinase, and optionally enhancing the enzyme activities of ribose 5-phosphate isomerase, allose 6-phosphate phosphatase, ribitol dehydrogenase, glycerol permease, glycerol dehydrogenase, and dihydroxyacetone kinase. A method for producing allulose and allose is an extracellular multienzyme cascade method. Multienzyme cascade catalysis and fermentation are coupled to improve the conversion rate of starch sugar or sucrose to the synthesized allulose.

IPC Classes  ?

• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12N 9/16 - Hydrolases (3.) acting on ester bonds (3.1)
• C12N 9/90 - Isomerases (5.)
• C12N 9/92 - Glucose isomerase
• C12P 19/02 - Monosaccharides
• C12P 19/24 - Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose

Abstract

Provided are a Corynebacterium glutamicum that produces L-lysine, a method for constructing the L-lysine-producing bacterium, and a method for preparing L-lysine using the bacterium. The lysine yield and glucose conversion rate are improved by using the L-lysine-producing bacterium, thereby reducing production costs.

IPC Classes  ?

• C07K 14/34 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria from Corynebacterium (G)
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
• C12R 1/15 - Corynebacterium
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression

Abstract

The present disclosure relates to D-xylulose 4-epimerase, a mutant thereof and use thereof. Specifically, the present disclosure relates to a polypeptide having D-xylulose 4-epimerase activity, a method for preparing the polypeptide, and use of the polypeptide in the preparation of L-pentose using D-xylose or D-xylulose as a raw material. Compared with the conventional production method in the prior art, the new method for preparing L-pentose discovered in the present disclosure has a simpler production process and reduces the costs of producing L-pentose.

IPC Classes  ?

• C12N 9/90 - Isomerases (5.)
• C12N 15/61 - Isomerases (5)
• C12P 19/24 - Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
• C12P 19/02 - Monosaccharides

Abstract

A construction method for genetically engineered bacteria of a filamentous fungus. The method comprises: enabling, by a genetic engineering method, a filamentous fungus to overexpress an ethanol synthesis positive regulatory gene, and/or down-regulate an endogenous ethanol synthesis negative regulatory gene; or in a cell of the filamentous fungus, overexpressing acetaldehyde dehydrogenase and alcohol dehydrogenase containing a mitochondrial localization signal sequence, or overexpressing pyruvate decarboxylase and alcohol dehydrogenase containing a mitochondrial localization signal sequence, or overexpressing acetaldehyde dehydrogenase, alcohol dehydrogenase, and pyruvate decarboxylase containing a mitochondrial localization signal sequence. Compared with the original strain, the obtained genetically engineered strain has improved ethanol synthesis capability.

IPC Classes  ?

• C12N 1/15 - Fungi Culture media therefor modified by introduction of foreign genetic material
• C12N 15/80 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi

Abstract

Synthesis and use of a protocatechuic acid-based epoxy resin. Specifically, a protocatechuic acid epoxidized monomer has a structure as shown in the following formula (I). The monomer can be cured to form a protocatechuic acid epoxy resin, thereby being used for preparing aerospace special materials.

IPC Classes  ?

• C08G 59/32 - Epoxy compounds containing three or more epoxy groups
• C07D 301/28 - Condensation of epihalohydrins or halohydrins with compounds containing active hydrogen atoms by reaction with hydroxyl radicals
• C07D 301/30 - Condensation of epihalohydrins or halohydrins with compounds containing active hydrogen atoms by reaction with carboxyl radicals
• C07D 303/30 - Ethers of oxirane-containing polyhydroxy compounds in which all hydroxyl radicals are etherified with oxirane-containing hydroxy compounds

Abstract

Provided is an MviN protein mutant, said mutant improving corynebacterium glutamic acid and lysine production capability by means of introducing an amino acid mutation at a set position in an MviN protein amino acid sequence.

IPC Classes  ?

• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12P 13/14 - Glutamic acidGlutamine
• C12P 13/08 - LysineDiaminopimelic acidThreonineValine
• C12R 1/15 - Corynebacterium

Abstract

Provided are base editing systems for achieving C to A and C to G base mutation and an application thereof. One of the base editing systems, in which C is mutated to A, comprises cytosine deaminase AID and nCas9 nuclease or comprises cytosine deaminase AID, nCas9 nuclease, and uracil DNA glycosidase. The other base editing system, in which C is mutated to G, comprises cytosine deaminase APOBEC, nCas9 nuclease, and uracil DNA glycosidase. Experiments have shown that a combination of three base editing systems, i.e., C to A, C to T, and A to G, can achieve a mutation of A, T, C, or G to any base in prokaryotes. A combination of three base editing systems, i.e., C to G, C to T, and A to G, can achieve a mutation of A, T, C, or G to any base in eukaryotes.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/90 - Stable introduction of foreign DNA into chromosome
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression

Abstract

Provided is a recombinant yeast expressing germacrene A synthetase or a fusion protein thereof, wherein the fusion protein is germacrene A synthetase and farnesyl pyrophosphate synthase. The recombinant yeast improves the yield of germacrene A, and is suitable for the industrialized production of β-elemene and/or germacrene A.

IPC Classes  ?

• C12N 1/18 - Baker's yeastBrewer's yeast
• C12N 9/10 - Transferases (2.)
• C12N 9/88 - Lyases (4.)
• C12R 1/865 - Saccharomyces cerevisiae

Abstract

Disclosed are a phosphoketolase with enhanced activity and a use thereof in metabolite production. A protein is provided, being a mutant protein obtained by subjecting a phosphoketolase to any one or more of the following mutations: T at position 2 is mutated to A; I at position 6 is mutated to T; N at position 14 is mutated to D; E at position 20 is mutated to D; T at position 120 is mutated to A, E at position 231 is mutated to K; H at position 260 is mutated to Y; E at position 342 is mutated to K; K at position 397 is mutated to R, D at position 676 is mutated to G, F at position 785 is mutated to L; and W at position 801 is mutated to R. Also disclosed is a use of the mutant protein in metabolite production. Compared to existing phosphoketolases, the mutant protein phosphoketolase provided has markedly enhanced enzyme activity, which can significantly increase the yield of a target metabolite.

IPC Classes  ?

• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12P 9/00 - Preparation of organic compounds containing a metal or atom other than H, N, C, O, S, or halogen
• C12P 7/24 - Preparation of oxygen-containing organic compounds containing a carbonyl group
• C12P 7/26 - Ketones
• C12P 19/32 - Nucleotides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same-ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide

Abstract

Rhizopus.

IPC Classes  ?

• C12P 7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
• C07K 14/37 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from fungi
• C12N 15/80 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/10 - Transferases (2.)
• C12P 7/44 - Polycarboxylic acids
• C12P 7/50 - Polycarboxylic acids having keto groups, e.g. 2-ketoglutaric acid
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12N 1/14 - Fungi Culture media therefor
• C12R 1/645 - Fungi

Abstract

A glutamic acid fermentation process, comprising the following steps: introducing Corynebacterium glutamicum into a fermentation tank filled with a clean fermentation medium for fermentation culture, performing ultrasonic treatment, and adjusting the pH value of the fermentation broth.

IPC Classes  ?

• C12P 13/18 - Glutamic acidGlutamine using biotin or its derivatives
• C12R 1/15 - Corynebacterium

Abstract

The present invention provides a class of new mutant proteins for increasing malic acid yield. Specifically, the present invention provides a class of new pyruvate carboxylase mutant protein and malic acid transporter mutant proteins or combinations thereof, a preparation method therefor and use thereof in improving malic acid yield.

IPC Classes  ?

• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12P 7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid

Abstract

An enzyme synthesizes hydroxyl acetaldehyde and/or 1,3-dihydroxyacetone by catalyzing formaldehyde. Site-directed mutation of benzoylformate decarboxylase (BFD) creates a mutant of the enzyme, which can polymerize the formaldehyde, A phosphoketalose (F/XPK) generates acetyl phosphoric acid from the hydroxyl acetaldehyde or 1,3-dihydroxyacetone (DHA). Combination with phosphotransacetylase (Pta) provides a route from the formaldehyde to acetyl coenzyme A in three steps.

IPC Classes  ?

• C12N 9/88 - Lyases (4.)
• C12P 7/24 - Preparation of oxygen-containing organic compounds containing a carbonyl group
• C12P 7/28 - Acetone-containing products
• C12P 9/00 - Preparation of organic compounds containing a metal or atom other than H, N, C, O, S, or halogen
• C12P 19/32 - Nucleotides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same-ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide

Abstract

The present invention belongs to the field of enzyme-catalyzed preparation of glucosamine. Provided is a method for preparing glucosamine, especially a method for preparing glucosamine by in vitro enzyme catalysis. The method comprises: catalyzing the conversion of fructose-6-phosphate (F6P) and an ammonium salt into glucosamine-6-phosphate (GlcN6P) using glucosamine-6-phosphate deaminase (EC 3.5.99.6, GlmD); and catalyzing the dephosphorylation of GlcN6P using an enzyme capable of catalyzing the dephosphorylation to produce glucosamine (GlcN).

IPC Classes  ?

• C12P 19/26 - Preparation of nitrogen-containing carbohydrates
• C12N 9/90 - Isomerases (5.)
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes

Abstract

Provided are a carbonyl reductase mutant protein and the use thereof in reduction of a cyclopentanedione compound. The mutant protein is a non-native protein, and can be used to catalyze a cyclopentanedione compound to generate a cyclopentanone alcohol. The mutant protein is mutated in four or more core amino acids, related to the enzyme catalytic activity, of a wild type carbonyl reductase.

IPC Classes  ?

• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12N 15/53 - Oxidoreductases (1)
• C12P 7/26 - Ketones

Abstract

A construction method and application of a recombinant strain for producing psicose and derivatives thereof. A recombinant strain and synthesized psicose, allose, and allitol are obtained by regulating the intracellular metabolism of glucose, decreasing the enzyme activity of fructose 6-phosphate kinase, and improving the enzyme activity of glucokinase and glucose-6-phosphate isomerase, the enzyme activity of 6-phosphate psicose 3-epimerase, 6-phosphate psicose phosphatase, fructose permease, and fructokinase, and any one of ribose-5-phosphate isomerase, 6-phosphate allose phosphatase, and ribitol dehydrogenase, and the enzyme activity of glycerol permease, glycerol dehydrogenase, and dihydroxyacetone kinase. A method for synthesizing psicose and allose by an extracellular multienzyme cascade method further couples a multienzyme cascade method to a fermentation method to improve the conversion rate that the starch sugar and sucrose are converted into the synthesized psicose. The method has a high conversion rate, is low in cost, and is suitable for producing synthesized psicose and derivates thereof in scale.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• C12N 1/20 - BacteriaCulture media therefor
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12P 19/24 - Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose

Abstract

Provided is a new use of an influenza virus antibody, in particular the use of an influenza virus antibody for preparing a drug against an H7 subtype influenza virus, wherein the light chain variable region of the influenza virus antibody has the amino acid sequence as set forth in SEQ ID NO.1; and the heavy chain variable region of the influenza virus antibody has the amino acid sequence as set forth in SEQ ID NO.2. The influenza virus antibody can bind well to a HA protein of the H7 subtype of the influenza virus, and especially has a good neutralization effect on the H7N9 influenza virus in vivo, and can prevent the infection with the H7N9 subtype influenza virus that leads to the death of mice.

IPC Classes  ?

• C07K 16/10 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
• C12N 15/13 - Immunoglobulins
• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
• A61K 39/42 - AntibodiesImmunoglobulinsImmune serum, e.g. antilymphocytic serum viral
• A61P 31/16 - Antivirals for RNA viruses for influenza or rhinoviruses

Abstract

An inositol preparation method by enzymatic catalysis uses starch and cellulose or substrates thereof as substrates. Raw materials are converted to inositol by in vitro multi-enzyme reaction system in one pot. The yield from the substrate to inositol is significantly improved by process optimization and adding new enzymes. The new enzymes can promote the phosphorolysis of starch or cellulose and utilization of glucose, which is the final production after the phosphorolysis of starch and cellulose. The inositol preparation method described herein has great potentials in industrial production of inositol because of high inositol yield, easy scale-up, low production cost, and lower impact to environment.

IPC Classes  ?

• C12P 7/02 - Preparation of oxygen-containing organic compounds containing a hydroxy group
• C12N 9/42 - Hydrolases (3.) acting on glycosyl compounds (3.2) acting on beta-1, 4-glucosidic bonds, e.g. cellulase

Abstract

121212 by using compounds as raw materials.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12P 19/42 - Cobalamins, i.e. vitamin B12, LLD factor
• C12R 1/19 - Escherichia coli

Abstract

Disclosed is a new tagatose-6-phosphate 4-epimerase, which is capable of converting fructose-6-phosphate into tagatose-6-phosphate and vice versa. Also disclosed is an application of the enzyme in tagatose production.

IPC Classes  ?

• C12N 9/90 - Isomerases (5.)
• C12N 15/61 - Isomerases (5)
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12P 19/24 - Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose

Abstract

The present invention is in the field of bioelectricity. The present invention provides energy generating systems, methods, and devices that are capable of converting chemical energy stored in sugars into useful electricity.

IPC Classes  ?

• H01M 8/16 - Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts

Abstract

Disclosed in the present invention are an enzyme for synthesizing hydroxyl acetaldehyde and/or 1,3-dihydroxyacetone by catalyzing formaldehyde, and applications thereof. In the present invention, by means of site-directed mutation of BFD, a mutant of the enzyme is found; and by means of the mutant of the enzyme, polymerization of the formaldehyde is implemented; in addition, by means of F/XPK, generation of acetyl phosphoric acid from the hydroxyl acetaldehyde or 1,3-dihydroxyacetone is implemented; with combination of phosphotransacetylase (Pta), a route from the formaldehyde to acetyl coenzyme A is achieved in three steps with the enzyme.

IPC Classes  ?

• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12P 9/00 - Preparation of organic compounds containing a metal or atom other than H, N, C, O, S, or halogen
• C12P 7/24 - Preparation of oxygen-containing organic compounds containing a carbonyl group
• C12P 7/26 - Ketones
• C12P 19/32 - Nucleotides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same-ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide

Abstract

Disclosed is a solid state biological reaction device, comprising a main tank (1), wherein the device further comprises a support (2) supporting the main tank (1), and the support (2) makes the main tank (1) be rotational in the horizontal position, and be statically cultured in the vertical position. The device is relatively simple, in particular, the mixing of materials uses the method of a vehicle-tank in combination with rotation, achieving the tank free conversion between the two different poses of vertical and the horizontal; and the device conducts the work of loading, inoculation, cultivation and transplantation and so on in the upright pose, and completes the work of sterilization and mixing of materials and so on in the horizontal pose. The device is not only quick to use and easy to move, but also omits the stirring system which occupies a lot of manufacturing costs, and is easy to use in large-scale production.

IPC Classes  ?

• C12M 1/16 - Apparatus for enzymology or microbiology containing, or adapted to contain, solid media
• C12M 1/04 - Apparatus for enzymology or microbiology with gas introduction means
• C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12N 1/14 - Fungi Culture media therefor
• C12N 1/18 - Baker's yeastBrewer's yeast

Abstract

rhizopus.

IPC Classes  ?

• C12P 7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
• C07K 14/37 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from fungi
• C12N 15/80 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/10 - Transferases (2.)
• C12P 7/44 - Polycarboxylic acids
• C12P 7/50 - Polycarboxylic acids having keto groups, e.g. 2-ketoglutaric acid
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12R 1/645 - Fungi

Abstract

Disclosed is a new approach for synthesizing an acetyl coenzyme A and a derivative product thereof (5-phosphoarabinose, acetyl phosphoric acid, acetyl phosphate, and an acetyl coenzyme A derivative compound) using glycolaldehyde. The approach comprises a reaction of glycolaldehyde and 3-phosphoglyceraldehyde under the catalysis of an enzyme to generate 5-phosphoarabinose, wherein the enzyme is selected from an aldolase, a transaldolase, an isoenzyme and a mutant enzyme thereof.

IPC Classes  ?

• C12P 19/02 - Monosaccharides
• C12P 9/00 - Preparation of organic compounds containing a metal or atom other than H, N, C, O, S, or halogen
• C12P 19/32 - Nucleotides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same-ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
• C12P 7/04 - Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic

Abstract

Provided is a recombinant yeast expressing germacrene A synthetase or a fusion protein thereof, wherein the fusion protein is germacrene A synthetase and farnesyl pyrophosphate synthase. The recombinant yeast improves the yield of germacrene A, and is suitable for the industrialized production of β-elemene and/or germacrene A.

IPC Classes  ?

• C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
• C12N 1/16 - YeastsCulture media therefor
• C12R 1/85 - Saccharomyces

Abstract

The present invention provides a polypeptide complex on the basis of Titin-Telethonin beta-pleated sheet structure as a polypeptide or protein drug carrier, a method of using the polypeptide complex, and a fusion protein complex thereof. The polypeptide complex is capable of maintaining the activity of polypeptide or protein drugs and prolonging the half-life period simultaneously.

IPC Classes  ?

• C07K 19/00 - Hybrid peptides
• C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
• C07K 14/605 - Glucagons
• C07K 14/655 - Somatostatins
• A61K 47/62 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
• A61K 38/00 - Medicinal preparations containing peptides
• A61P 3/10 - Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
• A61K 47/42 - ProteinsPolypeptidesDegradation products thereofDerivatives thereof, e.g. albumin, gelatin or zein
• A61K 9/00 - Medicinal preparations characterised by special physical form
• A61K 38/26 - Glucagons
• A61K 38/22 - Hormones
• A61K 38/31 - Somatostatins
• C07K 14/78 - Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]

Abstract

Disclosed is a solid state biological reaction device, comprising a main tank (1), wherein the device further comprises a support (2) supporting the main tank (1), and the support (2) makes the main tank (1) be rotational in the horizontal position, and be statically cultured in the vertical position. The device is relatively simple, in particular, the mixing of materials uses the method of a vehicle-tank in combination with rotation, achieving the tank free conversion between the two different poses of vertical and the horizontal; and the device conducts the work of loading, inoculation, cultivation and transplantation and so on in the upright pose, and completes the work of sterilization and mixing of materials and so on in the horizontal pose. The device is not only quick to use and easy to move, but also omits the stirring system which occupies a lot of manufacturing costs, and is easy to use in large-scale production.

IPC Classes  ?

• C12M 1/16 - Apparatus for enzymology or microbiology containing, or adapted to contain, solid media
• C12M 1/04 - Apparatus for enzymology or microbiology with gas introduction means
• C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor

Abstract

Disclosed is a preparation containing ergothioneine. The ergothioneine is provided by an extracellular ferment liquor and/or the concentration of the extracellular ferment liquor which is obtained by removing the mycelia from the ferment liquor produced through the liquid fementation of the mushroom, and the preparation comprises food, cosmetics and animal feed. The method for preparing the preparation comprises liquid-fermentation of the mushroom, removing the mycelia from the fermentation product, and selectively concentrating the extracellular ferment liquor obtained after removing the mycelia. The extracellular ferment liquor and/or the concentration of the extracellular ferment liquor obtained through the liquid fermentation of the mushroom are used in preparing the preparation containing ergothioneine.

IPC Classes  ?

• A61K 8/44 - Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfurSalts, esters or N-acylated derivatives thereof
• C12P 13/04 - Alpha- or beta-amino acids

Abstract

Provided is an inositol preparation method. By using starch and cellulose or substrates thereof as substrates, adding an enzyme capable of promoting hydrolysis of the starch or the cellulose and by using an enzyme of a by-product glucose, a multienzyme reaction system is established, and the substrates are converted into inositol. The conversion rates of raw materials and the yield of the inositol are improved.

IPC Classes  ?

• C12P 7/02 - Preparation of oxygen-containing organic compounds containing a hydroxy group

Abstract

Provided are an engineering strain for synthesizing a dibasic organic acid and preparation and application of same. The engineering strain introduces or up-regulates expression of a positive regulator gene for synthesis of a dibasic organic acid, and/or down-regulates expression of a negative regulator gene for synthesis of a dibasic organic acid; as compared with the origin strain of the engineering strain, the producing capability for producing the dibasic organic acid is improved. The dibasic organic acid comprises malic acid, succinic acid, fumaric acid, oxaloacetic acid, glutaric acid, and adipic acid; the expression product of the positive regulator gene comprises aspartate transaminase, glutamic-aspartate transport protein, C4-dicarboxylic transport protein, pyruvate carboxylase, malate dehydrogenase, and glucose transport protein; the expression product of the negative regulator gene comprises succinyl coenzyme A synthase and malic-α-ketoglutaric transport protein; and the origin strain comprises myceliophthora thermophila, thielavia terrestris, aspergillus, and rhizopus.

IPC Classes  ?

• C12N 1/15 - Fungi Culture media therefor modified by introduction of foreign genetic material
• C12N 15/80 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi
• C12P 7/50 - Polycarboxylic acids having keto groups, e.g. 2-ketoglutaric acid
• C12P 7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
• C12P 7/44 - Polycarboxylic acids
• C12N 9/10 - Transferases (2.)
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C07K 14/37 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from fungi
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12R 1/645 - Fungi