Abrégé

A Pichia pastoris strain with high-temperature resistance and a high methanol conversion rate. By mean of using a protoplast fusion method, the strain with a high methanol utilization rate, Pichia pastoris HTX-33 (CGMCC NO. 25207) is fused with a high-temperature-resistant Pichia manshurica strain screened from rotten wood from the vineyards of Yuanshi Vineyard in Yinchuan, Ningxia Province to obtain a hybrid strain that is resistant to high temperatures and efficiently utilizes methanol. The hybrid strain is identified as a Pichia pastoris strain and can achieve high biomass growth and high methanol utilization at 37°C by means of using methanol as the sole carbon source. The strain has the advantages of being green, environmentally-friendly, pollution-free, high in expression rate, low in cost, etc.

Classes IPC  ?

• C12N 1/16 - LevuresLeurs milieux de culture
• A23K 10/16 - Ajout de micro-organismes ou de leurs produits d’extraction, p. ex. de protéines provenant d’organismes unicellulaires, à des compositions de produits alimentaires

Abrégé

Provided a genetically modified yeast strain for producing succinic acid, which strain has the activity or an enhanced activity of an NADPH-dependent malate dehydrogenase (EC 1.1.1.82), and optionally also has the activity or an enhanced activity of at least one of the following: (i) soluble fumarate reductase (EC 4.2.1.2), (ii) a pyruvate carboxylase (EC 6.4.1.1), (iii) a fumarase (EC 4.2.1.2), and (iv) succinate transport protein; and a preparation method therefor, a method for producing succinic acid using same, and the use thereof.

Classes IPC  ?

• C12P 7/46 - Acides dicarboxyliques ayant au plus quatre atomes de carbone, p. ex. acide fumarique, acide maléique
• C07K 14/39 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de champignons provenant de levures
• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
• C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
• C12N 9/88 - Lyases (4.)

Abrégé

The present invention relates to a fusion protein comprising a nucleic acid binding protein and a method for capturing a specific nucleic acid using same. The fusion protein is formed by fusing a cytosine base editor and a uracil binding protein. The method is a method for capturing, from a sample, DNA (containing a PAM and containing cytosine C at a specific position) containing a specific target sequence, and comprises: contacting a sample containing target DNA with the fusion protein to capture the target DNA, and detecting the target DNA. Under the mediation of a sgRNA library, provided is a use of the fusion protein in capturing, from a sample, DNA containing a specific target sequence and/or detecting the DNA. Target DNA can be captured from a complex nucleic acid sample with high throughput and high sensitivity and specificity, and practical application value is provided.

Classes IPC  ?

• C07K 19/00 - Peptides hybrides
• C12N 9/24 - Hydrolases (3.) agissant sur les composés glycosyliques (3.2)
• C12N 9/78 - Hydrolases (3.) agissant sur les liaisons carbone-azote autres que les liaisons peptidiques (3.5)
• C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
• C12N 15/62 - Séquences d'ADN codant pour des protéines de fusion
• C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]

Abrégé

The present invention provides a genetically modified malic acid producing yeast strain, wherein the strain has or has enhanced malate transport protein activity and has or has enhanced NADPH-dependent malate dehydrogenase (EC 1.1.1.82) activity, optionally also has or has enhanced at least one of the following activities: (i) pyruvate carboxylase (EC 6.4.1.1) activity, (ii) phosphoenolpyruvate carboxykinase (EC 4.1.1.49) activity, (iii) phosphoenolpyruvate carboxylase activity, and (iv) biotin transport protein activity; and a preparation method thereof, a method for producing L-malic acid using the same, and use thereof.

Classes IPC  ?

• C12P 7/46 - Acides dicarboxyliques ayant au plus quatre atomes de carbone, p. ex. acide fumarique, acide maléique
• C07K 14/39 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de champignons provenant de levures
• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
• C12N 9/88 - Lyases (4.)

Abrégé

A new light-controlled repressor protein OptoLacl and a light-controlled gene expression system constructed on the basis of OptoLacl.

Classes IPC  ?

• C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
• C12N 15/75 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Bacillus
• C12R 1/19 - Escherichia coli
• C12R 1/125 - Bacillus subtilis
• C12R 1/07 - Bacillus

Abrégé

Provided are a gRNA mutant and the use thereof, and further provided are a method for constructing same and a base editor containing same. The gRNA mutant is used in the base editor, and can universally reduce a base editing window, thereby improving the specificity of gene editing, and achieving specific editing on one base.

Classes IPC  ?

• A61K 48/00 - Préparations médicinales contenant du matériel génétique qui est introduit dans des cellules du corps vivant pour traiter des maladies génétiquesThérapie génique
• C12N 9/22 - Ribonucléases
• C12N 9/78 - Hydrolases (3.) agissant sur les liaisons carbone-azote autres que les liaisons peptidiques (3.5)
• C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
• C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
• C12N 15/85 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules animales

Abrégé

Provided are a method for preparing starch using carbon dioxide, a recombinant microorganism, a method for constructing the recombinant microorganism, and a reagent. The method for preparing starch using carbon dioxide comprises: (1) providing energy and carbon sources for microbial cells on the basis of carbon dioxide and extracellular non-optical energy; and (2) generating starch within the microbial cells on the basis of at least one of up-regulated glucose-1-phosphate adenylyltransferase and starch synthase in the microbial cells. In this way, by utilizing non-optical energy, such as electric energy or hydrogen energy, starch can be effectively prepared inside the microbial cells by fixing carbon dioxide.

Classes IPC  ?

• C12P 19/04 - Polysaccharides, c.-à-d. composés contenant plus de cinq radicaux saccharide reliés entre eux par des liaisons glucosidiques
• C12N 9/10 - Transférases (2.)
• C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)

Abrégé

Provided in the present invention are a genetically modified pantoic-acid-producing strain having, or having enhanced, NADH-dependent acetohydroxy acid isomeroreductase, a method for producing same, a method for producing D-pantoic acid by using same, and the use thereof in the production of D-pantoic acid.

Classes IPC  ?

• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
• C12P 7/42 - Acides hydroxycarboxyliques
• C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
• C12R 1/19 - Escherichia coli

Abrégé

The present invention provides a bispecific antibody for a broad-spectrum novel coronavirus. The bispecific antibody of the present invention comprises a first targeting domain D1, which targets a first epitope of a SARS-CoV-2RBD domain; and a second targeting domain D2, which targets a second epitope of the SARS-CoV-2RBD domain. The bispecific antibody of the present invention has broad-spectrum neutralizing activity, can effectively neutralize SARS‐CoV‐2 and a variety of SARS‐CoV‐2 novel coronavirus variants having strong infectivity and high harmfulness, and therefore has great application value in prevention, treatment and/or detection of novel coronavirus infection.

Classes IPC  ?

• C07K 16/46 - Immunoglobulines hybrides
• C07K 16/10 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant de virus de virus à ARN
• C12N 15/13 - Immunoglobulines
• C12N 15/85 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules animales
• A61K 39/42 - AnticorpsImmunoglobulinesImmunsérum, p. ex. sérum antilymphocitaire viraux
• A61P 31/14 - Antiviraux pour le traitement des virus ARN
• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus

Abrégé

The present application provides a monoclonal antibody or an antigen-binding fragment thereof that specifically binds to the RBD domain of SARS-CoV or SARS-CoV-2, a preparation method therefor, and the use thereof. The monoclonal antibody or the antigen-binding fragment thereof can bind to the RBD antigen of SARS-CoV or SARS-CoV-2 with a high affinity, and can neutralize SARS-CoV and SARS-CoV-2 prototype strains and a series of SARS-CoV and SARS-CoV-2 mutant strains with a relatively high neutralization activity, thereby inhibiting the infections caused by the above strains. Therefore, the monoclonal antibody or the antigen-binding fragment thereof has great potential application value in clinical treatment, prevention and/or detection of infections caused by SARS-CoV and SARS-CoV-2 prototype strains and SARS-CoV and SARS-CoV-2 mutant strains.

Classes IPC  ?

• C07K 16/10 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant de virus de virus à ARN
• C12N 15/13 - Immunoglobulines
• C12N 15/85 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules animales
• C12N 5/10 - Cellules modifiées par l'introduction de matériel génétique étranger, p. ex. cellules transformées par des virus
• C12P 21/02 - Préparation de peptides ou de protéines comportant une séquence connue de plusieurs amino-acides, p. ex. glutathion
• A61K 39/42 - AnticorpsImmunoglobulinesImmunsérum, p. ex. sérum antilymphocitaire viraux
• A61P 31/14 - Antiviraux pour le traitement des virus ARN
• G01N 33/577 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet faisant intervenir des anticorps monoclonaux
• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus

Abrégé

A polynucleotide having promoter activity of a malate dehydrogenase gene (mdh gene), a transcription expression cassette, recombinant expression vector and recombinant host cell containing the polynucleotide having the promoter activity, a method for constructing a promoter mutant, a method for regulating the transcription of a target gene, a method for preparing a protein, and method for producing a target compound. The polynucleotide having the promoter activity is a mutant of an mdh gene promoter, and compared with a promoter of a wild-type mdh gene, the promoter activity of the mutant is significantly improved. After operably linking the mutant to a target gene, the expression efficiency of the target gene can be significantly improved, thereby effectively improving the yield and transformation rate of a target compound.

Classes IPC  ?

• C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
• C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
• C12N 15/67 - Méthodes générales pour favoriser l'expression
• C12P 13/04 - Alpha- ou bêta-amino-acides

Abrégé

A protein having an L-proline efflux function and the use thereof are provided. A method for producing L-proline or hydroxyproline by means of using a protein ThrE is used for producing L-proline by means of enhancing the activity of a polypeptide, having an L-proline efflux function, in an L-proline-producing strain. Alternatively, the method is used for producing hydroxyproline by means of weakening the activity of a polypeptide, having an L-proline efflux function, in L-proline-producing host cells and enhancing the activity of a proline hydroxylase.

Classes IPC  ?

• C12P 13/24 - ProlineHydroxyprolineHistidine
• C07K 14/34 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Corynebacterium (G)
• C12N 1/20 - BactériesLeurs milieux de culture
• C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
• C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase

Abrégé

Provided are a microorganism for producing a pantoic acid, and a construction method therefor and an application thereof. The microorganism for producing the pantoic acid is obtained by knocking out a gene in Escherichia coli and introducing an exogenous gene. The obtained microorganism is Escherichia coli that is registered in the China General Microbiological Culture Collection Center with an accession number of CGMCC No. 21699. A pantoic acid synthesis pathway has been opened up, and accumulation of the pantoic acid can be achieved in a fermentation process.

Classes IPC  ?

• C12P 7/42 - Acides hydroxycarboxyliques
• C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
• C12N 9/10 - Transférases (2.)
• C12N 9/88 - Lyases (4.)
• C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
• C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes

Abrégé

Provided is a recombinant yeast expressing germacrene A synthetase or a fusion protein thereof, wherein the fusion protein is germacrene A synthetase and farnesyl pyrophosphate synthase. The recombinant yeast improves the yield of germacrene A, and is suitable for the industrialized production of β-elemene and/or germacrene A.

Classes IPC  ?

• C12N 1/18 - Levure de boulangerieLevure de bière
• C12N 9/88 - Lyases (4.)
• C12N 9/10 - Transférases (2.)
• C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
• C12P 5/00 - Préparation des hydrocarbures

Abrégé

Provided is a method for biosynthesis of a 1,4-dihydroxy-2-butanone compound, using benzaldehyde lyase as a catalyst to catalyze a hydroxymethylation reaction of 3-hydroxypropanal and formaldehyde to generate 1,4-dihydroxy-2-butanone. Cheap and easily available aldehydes are used as a substrate to synthesize 1,4-dihydroxy-2-butanone, providing a green and sustainable method which has application value.

Classes IPC  ?

• C12P 7/26 - Cétones

Abrégé

Provided are polypeptides with tagatose 6-phosphate epimerase activity, polypeptides with tagatose 6-phosphate phosphatase activity, a composition comprising the polypeptides with tagatose 6-phosphate epimerase activity and the polypeptides with tagatose 6-phosphate phosphatase activity, a method for preparing tagatose from the composition, and use of the composition in the preparation of tagatose.

Classes IPC  ?

• C12N 9/90 - Isomérases (5.)
• C12P 19/24 - Préparation de composés contenant des radicaux saccharide préparés par action d'une isomérase, p. ex. fructose

Abrégé

The present invention provides an mreC mutant and use thereof in L-valine fermentative production. The mreC mutant is a protein obtained by mutating proline at position 150 in the wild-type mreC amino acid sequence into leucine. The introduction of an mreC point mutation into the microbial genome, i.e., a C-to-T mutation at position 449 of the coding region in the wild-type mreC gene nucleotide sequence, can significantly improve the production capacity and biomass of an engineering strain of a high valine production. The knock-out and introduction of related enzyme genes of the L-valine anaerobic fermentation pathway further provide proper carbon metabolic flows and reducing capacity balance control in the L-valine production process. By the coordinative regulation of multiple genes, a metabolic pathway suitable for L-valine fermentative production is formed, thus achieving a high-level synthesis of L-valine in microorganisms.

Classes IPC  ?

• C07K 14/245 - Escherichia (G)
• C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine

Abrégé

Provided are a polynucleotide having promoter activity and an application of the polynucleotide in producing an amino acid. Also disclosed are a transcription expression cassette, a recombinant expression vector, and a recombinant host cell which contain the polynucleotide, and a method for enhancing expression of a target gene, a method for preparing a protein, and a method for producing an amino acid. The polynucleotide having the promoter activity is a mutant of a polynucleotide having the sequence as shown in SEQ ID NO: 9. Compared with the polynucleotide having the sequence as shown in SEQ ID NO: 9, the promoter activity of the mutant is significantly enhanced, thereby promoting the stable and efficient expression of the target gene.

Classes IPC  ?

• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine
• C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
• C12N 9/06 - Oxydoréductases (1.), p. ex. luciférase agissant sur des composés contenant de l'azote comme donneurs (1.4, 1.5, 1.7)

Abrégé

Provided are a polypeptide with aspartate kinase activity and the use thereof in the production of an amino acid. Specifically, provided are a novel polypeptide with aspartate kinase activity, a recombinant polypeptide, a polynucleotide, a nucleic acid construct, a recombinant expression vector, a recombinant host cell, and a method for producing an amino acid. The polypeptide with aspartate kinase activity is a mutant mutated at one or more positions corresponding to positions 293, 294 and 307 of the amino acid sequence as shown in SEQ ID NO: 1. Compared with a polypeptide having the sequence as shown in SEQ ID NO: 1, the mutant polypeptide removes the feedback inhibition of lysine on aspartate kinase, has high aspartate kinase activity, and can be used for the stable and efficient production of lysine and derivatives thereof.

Classes IPC  ?

• C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine

Abrégé

A starch biosynthesis method may implement total artificial biosynthesis from simple compounds such as dihydroxyacetone, formaldehyde, formic acid and methanol to starch. By coupling with methods such as chemical reduction of carbon dioxide, even total artificial biosynthesis of starch taking carbon dioxide as a starting raw material can be implemented. The method can utilize carbon dioxide of high concentration and high density and electric energy and hydrogen energy of high energy density, is more suitable for an industrial production mode, and the production cycle is shortened from several months in farming to several days.

Classes IPC  ?

• C12P 19/18 - Préparation de composés contenant des radicaux saccharide préparés par action d'une transférase glycosylique, p. ex. alpha-, bêta- ou gamma-cyclodextrines
• C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)

Abrégé

The present invention belongs to the technical field of crystal products, and relates to high-purity crystalline D-tagatose, a composition comprising same, a method for preparing same, and use thereof. Specifically, the crystalline D-tagatose of the present invention has a purity of 98% or greater, a particle size of 150 μm or greater, an aspect ratio of 1.0-4.0, an angle of repose of 40° or less, and a bulk density of 0.7 g/mL or greater. Compared with commercially available products, the crystalline D-tagatose has more excellent product morphology, better fluidity, and insusceptibility to agglomeration, which is conducive to reducing storage and transportation costs. In addition, the method for preparing the crystalline D-tagatose of the present invention can freely regulate the particle size of the product, eliminating the need for costly devices and facilitating the industrial production of the crystalline D-tagatose.

Classes IPC  ?

• C07H 3/02 - Monosaccharides
• C07H 1/06 - SéparationPurification

Abrégé

Provided are a mutant of a Corynebacterium glutamicum glutamate dehydrogenase gene promoter and applications thereof. The mutant has improved promoter activity compared to a wild-type promoter. Hence, it can be used to enhance the expression of a target gene, for example, operably ligating the mutant with a glutamate dehydrogenase gene, and the expression intensity of the glutamate dehydrogenase can be enhanced, thereby improving the amino acid production efficiency of a recombinant strain.

Classes IPC  ?

• C12N 9/06 - Oxydoréductases (1.), p. ex. luciférase agissant sur des composés contenant de l'azote comme donneurs (1.4, 1.5, 1.7)
• C12P 13/24 - ProlineHydroxyprolineHistidine
• C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium

Abrégé

Disclosed are a mutant of a pyruvate carboxylase gene promoter of Corynebacterium glutamicum and applications thereof. The mutant has improved promoter activity compared with a wild-type promoter, and can be used for enhancing expression of a target gene, for example, operably ligating the mutant to a pyruvate carboxylase gene enhances the expression intensity of the pyruvate carboxylase, thereby improving the production efficiency of amino acids of the strain.

Classes IPC  ?

• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine
• C12P 13/24 - ProlineHydroxyprolineHistidine
• C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium

Abrégé

Disclosed are a variety of terminal deoxynucleotidyl transferases (TdTs) and variants thereof for de novo synthesis of a polynucleotide having a controlled sequence and for efficient and controllable synthesis of a nucleic acid molecule without dependence on a template. It is found that some amino acid residues of the TdT catalytic domain can be specifically modified so as to improve the ability of such modified TdT to synthesize a polynucleotide.

Classes IPC  ?

• C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
• C12P 19/34 - Polynucléotides, p. ex. acides nucléiques, oligoribonucléotides

Abrégé

Disclosed is a genetically modified yeast strain for producing malic acid. The strain has the activity or an enhanced activity of a malic acid transporter and has the activity or an enhanced activity of an NADPH-dependent malate dehydrogenase (EC 1.1.1.82); and also has the activity or an enhanced activity of at least one of the following: (i) a pyruvate carboxylase (EC 6.4.11); (ii) a phosphoenolpyruvate carboxykinase (EC 41.1.49); (iii) a phosphoenolpyruvate carboxylase; and (iv) a biotin transporter. Disclosed are a preparation method for the strain, a method for producing L-malic acid by means of using the strain, and the use thereof.

Classes IPC  ?

• C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
• C12N 1/16 - LevuresLeurs milieux de culture
• C12P 7/46 - Acides dicarboxyliques ayant au plus quatre atomes de carbone, p. ex. acide fumarique, acide maléique

Abrégé

Provided are a genetically modified yeast strain for producing succinic acid, which strain has the activity or an enhanced activity of an NADPH-dependent malate dehydrogenase (EC1.1.1.82), and optionally also has the activity or an enhanced activity of at least one of the following: (i) a soluble fumarate reductase (EC 4.2.1.2), (ii) a pyruvate carboxylase (EC 6.4.1.1), (iii) a fumarase (EC 4.2.1.2), and (iv) a succinic acid transporter; and a preparation method therefor, a method for producing succinic acid by using same, and the use thereof.

Classes IPC  ?

• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12P 7/46 - Acides dicarboxyliques ayant au plus quatre atomes de carbone, p. ex. acide fumarique, acide maléique
• C12R 1/85 - Saccharomyces

Abrégé

Provided are a recombinant microorganism constructed based on a lysine efflux protein, a method for constructing the recombinant microorganism, a lysine producing strain, and a method for producing lysine. The polypeptide having the sequence as shown in SEQ ID NO: 4 or SEQ ID NO: 6 can promote the extracellular discharge of lysine, and two novel lysine efflux proteins are provided for the transformation of a lysine high-producing strain. A recombinant microorganism is constructed based on the polypeptide having the sequence as shown in SEQ ID NO: 4 or SEQ ID NO: 6. The recombinant microorganism has significantly increased lysine yield and sugar-acid conversion rate, and is suitable for large-scale industrial production of lysine.

Classes IPC  ?

• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine
• C07K 14/195 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries
• C07K 14/21 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Pseudomonadaceae (F)
• C12R 1/15 - Corynebacterium
• C12R 1/19 - Escherichia coli

Abrégé

Provided are a method for preparing starch using carbon dioxide, a recombinant microorganism, a method for constructing the recombinant microorganism, and a reagent. The method for preparing starch using carbon dioxide comprises: (1) providing energy and carbon sources for microbial cells on the basis of carbon dioxide and extracellular non-optical energy; and (2) generating starch within the microbial cells on the basis of at least one of up-regulated glucose-1-phosphate adenylyltransferase and starch synthase in the microbial cells. In this way, by utilizing non-optical energy, such as electric energy or hydrogen energy, starch can be effectively prepared inside the microbial cells by fixing carbon dioxide.

Classes IPC  ?

• C12P 19/04 - Polysaccharides, c.-à-d. composés contenant plus de cinq radicaux saccharide reliés entre eux par des liaisons glucosidiques
• C12P 19/18 - Préparation de composés contenant des radicaux saccharide préparés par action d'une transférase glycosylique, p. ex. alpha-, bêta- ou gamma-cyclodextrines
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 1/32 - Procédés utilisant des alcools saturés inférieurs, c.-à-d. de C1 à C6, ou milieux de culture en contenant

Abrégé

Related are a recombinant microorganism for producing L-valine, a construction method and an application thereof. Through enhancing amino acid dehydrogenase activity of L-valine fermentation strain, and/or activating an Entner-Doudoroff (ED) metabolic pathway, a problem in L-valine fermentation process that reducing power is unbalanced is solved, thereby the titer and yield of L-valine produced by Escherichia coli are improved, and L-valine was produced by one-step anaerobic fermentation.

Classes IPC  ?

• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine
• C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
• C12N 1/20 - BactériesLeurs milieux de culture
• C12N 9/06 - Oxydoréductases (1.), p. ex. luciférase agissant sur des composés contenant de l'azote comme donneurs (1.4, 1.5, 1.7)

Abrégé

Related are a recombinant microorganism for producing L-valine, a construction method and an application thereof. Through transferring an amino acid dehydrogenase gene and/or activating activity of a transhydrogenase and/or a NAD kinase, reducing power of NADPH in cell is increased, the titer and yield of L-valine generated by Escherichia coli are improved, and the production of L-valine by one-step anaerobic fermentation is achieved.

Classes IPC  ?

• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine
• C12N 9/06 - Oxydoréductases (1.), p. ex. luciférase agissant sur des composés contenant de l'azote comme donneurs (1.4, 1.5, 1.7)
• C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
• C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
• C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
• C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
• C12N 15/90 - Introduction stable d'ADN étranger dans le chromosome
• C12N 1/20 - BactériesLeurs milieux de culture
• C12N 9/88 - Lyases (4.)
• C12N 9/10 - Transférases (2.)

Abrégé

Related are a recombinant microorganism for producing L-valine, a construction method and an application thereof. Through transferring an acetohydroxy acid reductoisomerase gene and/or an amino acid dehydrogenase gene into a microorganism, and enhancing activity of an acetohydroxy acid reductoisomerase and/or an amino acid dehydrogenase, the titer and yield of L-valine generated by Escherichia coli may be improved, and L-valine was produced by one-step anaerobic fermentation.

Classes IPC  ?

• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine
• C12N 9/06 - Oxydoréductases (1.), p. ex. luciférase agissant sur des composés contenant de l'azote comme donneurs (1.4, 1.5, 1.7)
• C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
• C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
• C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
• C12N 15/90 - Introduction stable d'ADN étranger dans le chromosome

Abrégé

The invention discloses a construction method of genetic engineering fungi of filamentous fungi. Through the genetic engineering method, the filamentous fungi overexpress the positive regulation genes of ethanol synthesis, and/or down regulate the negative regulation genes of endogenous ethanol synthesis to obtain genetic engineering strains. Or overexpression of acetaldehyde dehydrogenase and ethanol dehydrogenase containing mitochondrial localization signal sequence, or overexpression of pyruvate decarboxylase and ethanol dehydrogenase containing mitochondrial localization signal sequence, or overexpression of acetaldehyde dehydrogenase, ethanol dehydrogenase and pyruvate decarboxylase containing mitochondrial localization signal sequence in filamentous fungal cells. Compared with the original strain, the ethanol synthesis ability of the obtained genetically engineered strains are improved.

Classes IPC  ?

• C12N 15/80 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons
• C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
• C12N 1/14 - ChampignonsLeurs milieux de culture

Abrégé

Provided are a gRNA mutant and the use thereof, and further provided are a method for constructing same and a base editor containing same. The gRNA mutant is used in the base editor, and can universally reduce a base editing window, thereby improving the specificity of gene editing, and achieving specific editing on one base.

Classes IPC  ?

• C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens

Abrégé

According to the copolymer film and the enzyme catalysis self-assembly synthesis method therefor provided by the present invention, the copolymer film is prepared at a two-liquid-phase interface by means of the enzyme catalysis of a film forming monomer comprising a phenolic hydroxyl group, a film forming monomer comprising at least two amino groups, and a catalyst. In the present invention, operation steps are simple, reaction conditions are mild, the selection range of the film forming monomer is wide, and a film forming monomer can be selected according to application requirements to prepare a copolymer film having a corresponding function. The preparation method of the present invention is easy to upgrade from a laboratory to industrial mass production, and has a good application prospect.

Classes IPC  ?

• C08G 83/00 - Composés macromoléculaires non prévus dans les groupes
• C08J 5/18 - Fabrication de bandes ou de feuilles
• C12P 1/00 - Préparation de composés ou de compositions, non prévue dans les groupes , utilisant des micro-organismes ou des enzymesProcédés généraux de préparation de composés ou de compositions utilisant des micro-organismes ou des enzymes

Abrégé

A pyruvate dehydrogenase mutant. On the basis of an amino acid sequence as shown in SEQ ID NO: 1, the 217th site of the sequence is mutated into any one of alanine, aspartic acid, glutamate, leucine and proline. The mutant can improve the yield and conversion rate of L-amino acids in a strain, growth of the strain is not inhibited while the yield is improved, and a new way is provided for large-scale production of the L-amino acids.

Classes IPC  ?

• C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
• C12N 15/53 - Oxydoréductases (1)
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12P 13/04 - Alpha- ou bêta-amino-acides
• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine
• C12P 13/12 - MéthionineCystéineCystine
• C12P 13/06 - AlanineLeucineIsoleucineSérineHomosérine
• C12R 1/19 - Escherichia coli
• C12R 1/15 - Corynebacterium

Abrégé

Provided are a preparation method for an immobilized cell for tagatose production and a method for producing tagatose by using the immobilized cell. The preparation method for the immobilized cell comprises: mixing a fermentation broth of Escherichia coli or Bacillus subtilis that expresses α-glucan phosphorylase, phosphoglucomutase, glucose phosphate isomerase, tagatose 6-phosphate epimerase, and tagatose 6-phosphate phosphatase to obtain a fermentation mixture, adding inorganic soil and then performing uniform stirring, then adding a flocculant to flocculate bacteria, subsequently adding a cross-linking agent to cross-link, performing vacuum filtration to obtain a filter cake, using a rotary granulator to extrude the filter cake to granulate into a long strip, then cutting by means of a spherical shot blasting machine into particles having equal lengths, and performing boiling drying to obtain the immobilized cell for tagatose production. According to the present invention, separation and purification steps of an enzyme required in tagatose production are simplified, the recycling rate of the enzyme is improved, and the recycling of the enzyme is achieved.

Classes IPC  ?

• C12N 11/14 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support inorganique
• C12N 11/10 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique le support étant un hydrate de carbone
• C12N 11/089 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique le support étant un polymère synthétique obtenu autrement que par des réactions faisant intervenir uniquement des liaisons non saturées carbone-carbone
• C12N 11/087 - Polymères acryliques
• C12N 11/082 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique le support étant un polymère synthétique obtenu par des réactions faisant intervenir uniquement des liaisons non saturées carbone-carbone
• C12P 19/02 - Monosaccharides
• C12R 1/19 - Escherichia coli
• C12R 1/125 - Bacillus subtilis

Abrégé

A method for preparing immobilized cells for producing mannose, and a method using same for producing mannose, comprising: fermenting to separately obtain fermentation broths of Escherichia coli or Bacillus subtilis expressing α-glucan phosphorylase, phosphoglucomutase, glucose phosphoisomerase, mannose-6-phosphate isomerase and mannose-6-phosphate phosphatase, and mixing the fermentation broths to obtain a mixed fermentation broth.

Classes IPC  ?

• C12N 11/14 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support inorganique
• C12N 11/10 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique le support étant un hydrate de carbone
• C12N 11/089 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique le support étant un polymère synthétique obtenu autrement que par des réactions faisant intervenir uniquement des liaisons non saturées carbone-carbone
• C12N 11/087 - Polymères acryliques
• C12N 11/082 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique le support étant un polymère synthétique obtenu par des réactions faisant intervenir uniquement des liaisons non saturées carbone-carbone
• C12P 19/02 - Monosaccharides
• C12R 1/19 - Escherichia coli
• C12R 1/125 - Bacillus subtilis

Abrégé

Disclosed is a mutant of a corynebacterium glutamicum dihydrodipicolinate reductase (dapB) gene promoter. Compared with the promoter of a wild-type dapB gene, the promoter activity of the mutant is significantly improved. Also disclosed are a transcription expression cassette comprising the promoter mutant, a recombinant expression vector, a recombinant host cell, and a method for constructing a promoter mutant, a method for regulating and controlling transcription of a target gene, a method for preparing a protein, and a method for producing a target compound.

Classes IPC  ?

• C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
• C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine
• C12R 1/01 - Bactéries ou actinomycètes

Abrégé

A polynucleotide having promoter activity of a malate dehydrogenase gene (mdh gene), a transcription expression cassette, recombinant expression vector and recombinant host cell containing the polynucleotide having the promoter activity, a method for constructing a promoter mutant, a method for regulating the transcription of a target gene, a method for preparing a protein, and method for producing a target compound. The polynucleotide having the promoter activity is a mutant of an mdh gene promoter, and compared with a promoter of a wild-type mdh gene, the promoter activity of the mutant is significantly improved. After operably linking the mutant to a target gene, the expression efficiency of the target gene can be significantly improved, thereby effectively improving the yield and transformation rate of a target compound.

Classes IPC  ?

• C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
• C12N 15/09 - Technologie d'ADN recombinant
• C12N 1/20 - BactériesLeurs milieux de culture
• C07K 14/34 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Corynebacterium (G)
• C12N 15/67 - Méthodes générales pour favoriser l'expression
• C12R 1/15 - Corynebacterium
• C12R 1/185 - Escherichia
• C12R 1/13 - Brevibacterium
• C12R 1/06 - Arthrobacter

Abrégé

The present invention discloses base editing systems for mutating a base C to A and a base C to G and applications thereof. The base editing system for mutating C to A disclosed in the present invention includes cytosine deaminase AID and nCas9 nuclease or includes cytosine deaminase AID, nCas9 nuclease and uracil DNA glycosidase; the base editing system for mutating C to G of the present invention includes cytosine deaminase APOBEC, nCas9 nuclease and uracil DNA glycosidase. The experiments show that a combination of the three base editing systems for mutating C to A, C to T and A to G can realize a mutation of A, T, C or G to any base in both prokaryotes and eukaryotes.

Classes IPC  ?

• C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
• C12N 15/90 - Introduction stable d'ADN étranger dans le chromosome
• C12N 9/22 - Ribonucléases
• C12N 9/78 - Hydrolases (3.) agissant sur les liaisons carbone-azote autres que les liaisons peptidiques (3.5)
• C12N 9/24 - Hydrolases (3.) agissant sur les composés glycosyliques (3.2)

Abrégé

Provided are a biomimetic silicon mineralized microcapsule immobilized multi-enzyme, a preparation method therefor, and a method for producing tagatose by using same. The preparation method comprises the following steps: (1) pre-mixing glucan phosphorylase, phosphoglucomutase, phosphoglucoisomerase, 6-phosphate tagatose 4-position epimerase and 6-phosphate tagatose phosphatase solutions, then adding the mixture to a calcium chloride solution, and then pouring same into a sodium carbonate solution, stirring and separating same to obtain calcium carbonate microspheres containing a multi-enzyme; (2) mixing the calcium carbonate microspheres with a polyethyleneimine solution to obtain polyethyleneimine-calcium carbonate microspheres after separation; (3) mixing the polyethyleneimine-calcium carbonate microspheres with a silicate solution to obtain biomimetic silicon mineralized-calcium carbonate microspheres after separation; and (4) mixing the biomimetic silicon mineralized-calcium carbonate microspheres with ethylenediamine tetraacetic acid for reaction to remove calcium carbonate, and separating same to obtain a biomimetic silicon mineralized microcapsule immobilized multi-enzyme.

Classes IPC  ?

• C12N 11/18 - Systèmes multi-enzymatiques
• C12N 11/14 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support inorganique
• C12N 11/089 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique le support étant un polymère synthétique obtenu autrement que par des réactions faisant intervenir uniquement des liaisons non saturées carbone-carbone
• C12N 11/04 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique piégées à l’intérieur du support, p. ex. dans un gel ou dans des fibres creuses
• C12P 19/24 - Préparation de composés contenant des radicaux saccharide préparés par action d'une isomérase, p. ex. fructose
• C12P 19/18 - Préparation de composés contenant des radicaux saccharide préparés par action d'une transférase glycosylique, p. ex. alpha-, bêta- ou gamma-cyclodextrines
• C12P 19/16 - Préparation de composés contenant des radicaux saccharide préparés par action d'une alpha-1, 6 glucosidase, p. ex. amylose, amylopectine déramifiée
• C12P 19/02 - Monosaccharides

Abrégé

Provided are the immobilization of multiple enzymes on the basis of an artificial oil body and an application thereof in the preparation of tagatose. Specifically, an artificial oil body is used to mix an expressed fusion protein of target protease-oil body protein with an oil body, which then undergoes an ultrasonic treatment; the fusion protein is anchored to the surface of the oil body by means of the specific hydrophobicity of a human protein to form an artificial oil body containing the target protease, so that the purification and immobilization of enzymes can be completed simultaneously. The immobilized multiple enzymes that can be used for tagatose production utilize an artificial oil body as an immobilized enzyme substrate, which significantly improves the stability of the immobilized enzymes, reduces the production cost of the current enzymatic preparation of tagatose, and has a simple preparation process.

Classes IPC  ?

• C12N 11/18 - Systèmes multi-enzymatiques
• C12N 11/06 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique attachées au support au moyen d'un agent de pontage
• C12P 19/24 - Préparation de composés contenant des radicaux saccharide préparés par action d'une isomérase, p. ex. fructose
• C12P 19/18 - Préparation de composés contenant des radicaux saccharide préparés par action d'une transférase glycosylique, p. ex. alpha-, bêta- ou gamma-cyclodextrines
• C12P 19/16 - Préparation de composés contenant des radicaux saccharide préparés par action d'une alpha-1, 6 glucosidase, p. ex. amylose, amylopectine déramifiée
• C12P 19/02 - Monosaccharides

Abrégé

Provided are a method for preparing an immobilized multi-enzyme system, and a method for producing tagatose by the immobilized multi-enzyme system. The immobilized multi-enzyme system is formed by uniformly mixing a porous dopamine microsphere with a multi-enzyme mixture which is used for producing tagatose. Five enzymes in an enzymatic catalysis path for converting starch to tagatose are co-immobilized by means of a porous microsphere to obtain an immobilized multi-enzyme system, the immobilized multi-enzyme system is used to catalyze conversion of starch into tagatose, and thus, enzymes can be recycled, thereby greatly reducing the amount of enzymes required for preparation of tagatose, and reducing the production cost.

Classes IPC  ?

• C12N 11/18 - Systèmes multi-enzymatiques
• C12N 11/04 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique piégées à l’intérieur du support, p. ex. dans un gel ou dans des fibres creuses
• C12P 19/24 - Préparation de composés contenant des radicaux saccharide préparés par action d'une isomérase, p. ex. fructose
• C12P 19/02 - Monosaccharides

Abrégé

Disclosed are an aspartate kinase gene expression regulatory sequence and the use thereof. By modifying positions 366-373 of a sequence represented by SEQ ID NO: 1, a polynucleotide formed by linking the sequence with a start codon GTG or TTG has transcriptional expression regulation activity, and these polynucleotides can improve expression of the aspartate kinase encoding gene lysC, such that a large amount of L-lysine is accumulated.

Classes IPC  ?

• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
• C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
• C12N 15/67 - Méthodes générales pour favoriser l'expression
• C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
• C12P 7/44 - Acides polycarboxyliques
• C12P 13/00 - Préparation de composés organiques contenant de l'azote
• C12P 13/06 - AlanineLeucineIsoleucineSérineHomosérine
• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine
• C12P 13/12 - MéthionineCystéineCystine

Abrégé

Provided are a microorganism for producing a pantoic acid, and a construction method therefor and an application thereof. The microorganism for producing the pantoic acid is obtained by knocking out a gene in Escherichia coli and introducing an exogenous gene. The obtained microorganism is Escherichia coli that is registered in the China General Microbiological Culture Collection Center with an accession number of CGMCC No. 21699. A pantoic acid synthesis pathway has been opened up, and accumulation of the pantoic acid can be achieved in a fermentation process.

Classes IPC  ?

• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 15/31 - Gènes codant pour des protéines microbiennes, p. ex. entérotoxines
• C12P 13/00 - Préparation de composés organiques contenant de l'azote
• C12R 1/19 - Escherichia coli

Abrégé

Disclosed are an aspartate kinase gene expression regulatory sequence and the use thereof. By modifying positions 366-373 of a sequence represented by SEQ ID NO: 1, a polynucleotide formed by linking the sequence with a start codon GTG or TTG has transcriptional expression regulation activity, and these polynucleotides can improve expression of the aspartate kinase encoding gene lysC, such that a large amount of L-lysine is accumulated.

Classes IPC  ?

• C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
• C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
• C12N 15/67 - Méthodes générales pour favoriser l'expression
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine
• C12P 13/06 - AlanineLeucineIsoleucineSérineHomosérine
• C12P 13/12 - MéthionineCystéineCystine
• C12P 13/00 - Préparation de composés organiques contenant de l'azote
• C12P 7/44 - Acides polycarboxyliques
• C12R 1/15 - Corynebacterium

Abrégé

The present invention relates to the field of bioengineering, and specifically to genetically engineered bacillus subtilis for efficient exogenous protein expression and high-density culture. Genetic modification is performed on histidine-deficient bacillus subtilis, and the function of hisC gene is recovered by means of genetic manipulation, to obtain a non-auxotrophic strain. Further, a strain obtained by knocking out genes spoIIAC and srfAC is more suitable for expression of a variety of exogenous proteins. The non-auxotrophic bacillus subtilis for expressing exogenous proteins obtained in the present invention can provide a low-cost high-density fermentation technology, so as to greatly lower the fermentation cost and achieve high-level expression of exogenous proteins.

Classes IPC  ?

• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 15/54 - Transférases (2)
• C12N 9/10 - Transférases (2.)
• C12N 15/75 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Bacillus
• C12R 1/125 - Bacillus subtilis

Abrégé

Provided are a polynucleotide having promoter activity and use thereof in the production of target compounds. Specifically, provided are a polynucleotide having promoter activity, a transcriptional expression cassette, recombinant expression vector, recombinant host cell comprising the polynucleotide having promoter activity, a method for regulating the transcription of a target gene, a method for preparing a protein, and a method for producing target compounds. The provided polynucleotide having promoter activity is a high salt and high osmolality-inducible promoter, and has enhanced promoter activity in an environment of increased salt concentration and osmolality. The polynucleotide is operatively connected to the target gene, can significantly improve the expression intensity of the target gene in the stress environment of high salt and high osmolality, thereby stably and efficiently producing a downstream product, and effectively solving the current problems of adding expensive inducers such as IPTG and causing toxicity to a strain.

Classes IPC  ?

• C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
• C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12P 13/04 - Alpha- ou bêta-amino-acides
• C12P 7/44 - Acides polycarboxyliques
• C12R 1/15 - Corynebacterium

Abrégé

Disclosed in the present invention are a protein having an L-proline efflux function, and the use thereof. A method for producing L-proline or hydroxyproline by means of using a protein ThrE is used for producing L-proline by means of enhancing the activity of a polypeptide, having an L-proline efflux function, in an L-proline-producing strain. Alternatively, the method is used for producing hydroxyproline by means of weakening the activity of a polypeptide, having an L-proline efflux function, in L-proline-producing host cells and enhancing the activity of a proline hydroxylase.

Classes IPC  ?

• C12P 13/24 - ProlineHydroxyprolineHistidine
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger

Abrégé

Provided are a bacillus subtilis genetically engineered bacterium for producing tagatose and a method for preparing tagatose. The genetically engineered bacterium comprises constructing thermostable α-glucan phosphorylases, thermostable glucose phosphomutases, thermostable glucose phosphate isomerases, thermostable 6-tagatose phosphate epimerases, and thermostable 6-tagatose phosphate phosphatases which are independently expressed or co-expressed. The usage of the genetically engineered bacterium can effectively convert starch into tagatose. Compared with existing methods for producing tagatose, the method has advantages such as suitability for whole-cell recycling, high safety, high yield, simple production process, low cost, and easiness in large-scale preparation.

Classes IPC  ?

• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 15/75 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Bacillus
• C12N 11/14 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support inorganique
• C12P 19/02 - Monosaccharides
• C12R 1/125 - Bacillus subtilis

Abrégé

Disclosed is a method for producing at least one target product from glycolic acid under the action of an enzyme. In order to solve the key problem of losing 25% fixed organic carbon during the recycling process of natural glycolic acid in C3 plants, the present application designs a glycolic acid metabolic pathway comprising an acetate kinase, a phosphoacetyl transferase, a glycolyl coenzyme A reductase and an acetyl phosphate synthase; or the pathway comprises a glycolyl coenzyme A synthase, a glycolyl coenzyme A reductase, an acetyl phosphate synthase and a phosphoacetyl transferase. A new glycolic acid metabolic pathway reduces the loss of organic carbon caused by the recycling process of glycolic acid, and converts 100% of the byproduct glycolic acid generated by photosynthesis into acetyl coenzyme A, thereby providing a new idea for improving the photosynthesis of plants. The pathway also provides a method for preparing glycolaldehyde or acetyl coenzyme A by using glycolic acid as a raw material.

Classes IPC  ?

• C12P 9/00 - Préparation de composés organiques contenant un métal ou un atome autre que H, N, C, O, S ou un halogène
• C12P 17/16 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle contenant plusieurs hétérocycles
• C12P 7/24 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carbonyle
• C12N 9/10 - Transférases (2.)
• C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
• C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
• A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
• A01H 6/46 - Gramineae ou Poaceae, p. ex. ivraie, riz, blé ou maïs
• A01H 6/54 - Leguminosae ou Fabaceae, p. ex. soja, luzerne ou arachide
• A01H 6/82 - Solanaceae, p. ex. poivron, tabac, pomme de terre, tomate ou aubergine
• A01H 6/38 - Euphorbiaceae, p. ex. Poinsettia

Abrégé

Provided are a recombinant microorganism modified by means of genetic engineering and an application of the recombinant microorganism in production of tagatose, a preparation method for the recombinant microorganism, a tagatose production strain and a tagatose production method. According to the recombinant microorganism, the tagatose is produced by taking glucose as a substrate or taking glycerol and glucose as substrates; the efficiency of conversion and production of tagatose by means of the recombinant microorganism is high, and a multi-enzyme purification process step is not needed.

Classes IPC  ?

• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 1/19 - LevuresLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12P 19/02 - Monosaccharides
• C12R 1/19 - Escherichia coli
• C12R 1/15 - Corynebacterium
• C12R 1/125 - Bacillus subtilis
• C12R 1/225 - Lactobacillus
• C12R 1/865 - Saccharomyces cerevisiae

Abrégé

Provided are a polypeptide with aspartate kinase activity and the use thereof in the production of an amino acid. Specifically, provided are a novel polypeptide with aspartate kinase activity, a recombinant polypeptide, a polynucleotide, a nucleic acid construct, a recombinant expression vector, a recombinant host cell, and a method for producing an amino acid. The polypeptide with aspartate kinase activity is a mutant mutated at one or more positions corresponding to positions 293, 294 and 307 of the amino acid sequence as shown in SEQ ID NO: 1. Compared with a polypeptide having the sequence as shown in SEQ ID NO: 1, the mutant polypeptide removes the feedback inhibition of lysine on aspartate kinase, has high aspartate kinase activity, and can be used for the stable and efficient production of lysine and derivatives thereof.

Classes IPC  ?

• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 1/20 - BactériesLeurs milieux de culture
• C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
• C12N 15/54 - Transférases (2)
• C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine

Abrégé

The present invention relates to a use of a degradable composite material in oil absorption. The degradable composite material comprises a three-dimensional net-shaped structure formed by fungal hyphae and at least one lignocellulose fragment fixed by the three-dimensional net-shaped structure. The degradable composite material has good oil absorption performance and has good application potential in the aspects of petroleum leakage, removal of oil stains on water, treatment of oily wastewater, and the like. Furthermore, the problem of treatment of agricultural and forestry residues such as straw and wood chips can be solved, the additional value of the residues is increased, environmental pollution is reduced, and coordinated development of economy, ecological environment, and society is facilitated.

Classes IPC  ?

• C02F 1/40 - Dispositifs pour séparer ou enlever les substances grasses ou huileuses, ou les matières flottantes similaires
• C02F 1/28 - Traitement de l'eau, des eaux résiduaires ou des eaux d'égout par absorption ou adsorption
• B01J 20/24 - Composés macromoléculaires d'origine naturelle, p. ex. acides humiques ou leurs dérivés
• B01J 20/30 - Procédés de préparation, de régénération ou de réactivation
• C08L 97/02 - Matériau lignocellulosique, p. ex. bois, paille ou bagasse
• C09K 3/32 - Substances non couvertes ailleurs pour traiter les polluants liquides, p. ex. le pétrole, l'essence ou les corps gras
• D03D 15/00 - Tissus caractérisés par la matière, la structure ou les propriétés des fibres, des filaments, des filés, des fils ou des autres éléments utilisés en chaîne ou en trame
• C12N 11/08 - Enzymes ou cellules microbiennes immobilisées sur ou dans un support organique le support étant un polymère synthétique

Abrégé

A use of a degradable composite material for humidity control. The degradable composite material comprises a three-dimensional network structure formed by fungal hyphae, and at least one type of lignocellulose debris fixed by the three-dimensional network structure. A composite structure of the hyphae and the lignocellulose debris is formed as the hyphae grow and entangle the lignocellulose debris. The material has good moisture absorption and moisture liberation capabilities and can be used as a humidity control material. In addition, the composite material is a natural degradable organic humidity control material, which is green and safe.

Classes IPC  ?

• C08L 97/02 - Matériau lignocellulosique, p. ex. bois, paille ou bagasse
• C08L 101/16 - Compositions contenant des composés macromoléculaires non spécifiés les composés macromoléculaires étant biodégradables
• C12N 1/14 - ChampignonsLeurs milieux de culture
• C09K 3/00 - Substances non couvertes ailleurs
• C12R 1/645 - Champignons

Abrégé

Use of a mycelium material in oil absorption. The mycelium material is composed of hyphae of fungi. The mycelium material has relatively good oil absorption performance, and thus has good application potential in oil leakages, removal of oil pollutant on water, oily wastewater treatment, adsorption of oil mist in gas, and so on.

Classes IPC  ?

• C02F 3/34 - Traitement biologique de l'eau, des eaux résiduaires ou des eaux d'égout caractérisé par les micro-organismes utilisés

Abrégé

Provided is a starch biosynthesis method. The method may implement total artificial biosynthesis from simple compounds such as dihydroxyacetone, formaldehyde, formic acid and methanol to starch, and by coupling with methods such as chemical reduction of carbon dioxide, even total artificial biosynthesis of starch taking carbon dioxide as a starting raw material can be implemented. Natural starch synthesis is required to be subjected to the Calvin cycle, needing 21-22 reaction steps in total, and the present method merely needs 9-12 reaction steps. Nearly a half of the reaction steps is reduced, and a production cycle is greatly shortened. In addition, the present method can utilize carbon dioxide having high concentration and high density and electric energy and hydrogen energy having high energy density, is more suitable for an industrial production mode, and the production cycle is shortened from several months in farming to several days.

Classes IPC  ?

• C12P 19/04 - Polysaccharides, c.-à-d. composés contenant plus de cinq radicaux saccharide reliés entre eux par des liaisons glucosidiques
• C12P 19/14 - Préparation de composés contenant des radicaux saccharide préparés par action d'une carbohydrase, p. ex. par action de l'alpha-amylase
• C12N 15/54 - Transférases (2)

Abrégé

Provided are a polynucleotide having promoter activity and an application of the polynucleotide in producing an amino acid. Also disclosed are a transcription expression cassette, a recombinant expression vector, and a recombinant host cell which contain the polynucleotide, and a method for enhancing expression of a target gene, a method for preparing a protein, and a method for producing an amino acid. The polynucleotide having the promoter activity is a mutant of a polynucleotide having the sequence as shown in SEQ ID NO: 9. Compared with the polynucleotide having the sequence as shown in SEQ ID NO: 9, the promoter activity of the mutant is significantly enhanced, thereby promoting the stable and efficient expression of the target gene.

Classes IPC  ?

• C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
• C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
• C12N 15/53 - Oxydoréductases (1)
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine
• C12R 1/15 - Corynebacterium

Abrégé

Provided are a polynucleotide having promoter activity and an application of the polynucleotide in producing an amino acid. Also disclosed are a transcription expression cassette, a recombinant expression vector, and a recombinant host cell which contain the polynucleotide, and a method for enhancing expression of a target gene, a method for preparing a protein, and a method for producing an amino acid. The polynucleotide having the promoter activity is a mutant of a polynucleotide having the sequence as shown in SEQ ID NO: 9. Compared with the polynucleotide having the sequence as shown in SEQ ID NO: 9, the promoter activity of the mutant is significantly enhanced, thereby promoting the stable and efficient expression of the target gene.

Classes IPC  ?

• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
• C12N 15/53 - Oxydoréductases (1)
• C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine

Abrégé

Provided are a mutant of a Corynebacterium glutamicum glutamate dehydrogenase gene promoter and an application thereof. The mutant has improved promoter activity compared to a wild-type promoter. Hence, same can be used to enhance the expression of a target gene, for example, operably linking same with a glutamate dehydrogenase gene, and the expression intensity of the glutamate dehydrogenase can be enhanced, thereby improving the amino acid production efficiency of a recombinant strain.

Classes IPC  ?

• C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
• C12N 15/67 - Méthodes générales pour favoriser l'expression
• C12P 13/04 - Alpha- ou bêta-amino-acides
• C12P 13/14 - Acide glutamiqueGlutamine

Abrégé

Disclosed are a mutant of a pyruvate carboxylase gene promoter of Corynebacterium glutamicum and use thereof. The mutant has improved promoter activity compared with a wild-type promoter, and can be used for enhancing expression of a target gene, for example, operably connecting the mutant to a pyruvate carboxylase gene enhances the expression intensity of the pyruvate carboxylase, thereby improving the production efficiency of amino acids of the strain.

Classes IPC  ?

• C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
• C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
• C12N 15/67 - Méthodes générales pour favoriser l'expression
• C12P 13/04 - Alpha- ou bêta-amino-acides
• C12R 1/15 - Corynebacterium

Abrégé

Disclosed are a mutant of a pyruvate carboxylase gene promoter of Corynebacterium glutamicum and use thereof. The mutant has improved promoter activity compared with a wild-type promoter, and can be used for enhancing expression of a target gene, for example, operably connecting the mutant to a pyruvate carboxylase gene enhances the expression intensity of the pyruvate carboxylase, thereby improving the production efficiency of amino acids of the strain.

Classes IPC  ?

• C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
• C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
• C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
• C12P 13/04 - Alpha- ou bêta-amino-acides

Abrégé

Provided are an allulose 3-epimerase mutant and a genetically engineered bacterium expressing the mutant. Further provided are an immobilized allulose 3-epimerase enzyme and an immobilization method thereof. A high-throughput screening method is used to obtain an allulose 3-epimerase mutant efficiently expressed in a fermentation process, which can catalyze efficient conversion of fructose to D-allulose, providing an efficient production path for key enzymes required in a D-allulose production process. Additionally, the allulose 3-epimerase is bonded to an immobilizing resin to prepare an immobilized allulose 3-epimerase enzyme. The immobilized enzyme can be applied to batch or continuous reactions to catalyze efficient conversion of fructose to D-allulose, improves the reuse rate and useful life of the enzyme, can significantly reduce the production costs of D-allulose, and can be widely applied.

Classes IPC  ?

• C12N 9/90 - Isomérases (5.)
• A23L 29/30 - Aliments ou produits alimentaires contenant des additifsLeur préparation ou leur traitement contenant des sirops d'hydrate de carboneAliments ou produits alimentaires contenant des additifsLeur préparation ou leur traitement contenant des sucresAliments ou produits alimentaires contenant des additifsLeur préparation ou leur traitement contenant des alcools de sucre, p. ex. du xylitolAliments ou produits alimentaires contenant des additifsLeur préparation ou leur traitement contenant des hydrolysats d'amidon, p. ex. de la dextrine
• C12P 19/24 - Préparation de composés contenant des radicaux saccharide préparés par action d'une isomérase, p. ex. fructose

Abrégé

The present invention provides a class of new mutant proteins for increasing malic acid yield. Specifically, the present invention provides a class of new pyruvate carboxylase mutant protein and malic acid transporter mutant proteins or combinations thereof, a preparation method therefor and use thereof in improving malic acid yield.

Classes IPC  ?

• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C07K 14/37 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de champignons
• C12P 7/46 - Acides dicarboxyliques ayant au plus quatre atomes de carbone, p. ex. acide fumarique, acide maléique

Abrégé

Provided are a recombinant microorganism for producing L-valine, a construction method therefor, and an application thereof. By introducing an amino acid dehydrogenase gene and/or activating the activity of transhydrogenase and/or NAD kinase, cell NADPH reducing power is increased, the yield and conversion rate of L-valine produced by Escherichia coli are improved, and one-step anaerobic fermentation of L-valine is achieved.

Classes IPC  ?

• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 9/06 - Oxydoréductases (1.), p. ex. luciférase agissant sur des composés contenant de l'azote comme donneurs (1.4, 1.5, 1.7)
• C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
• C12N 9/90 - Isomérases (5.)
• C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
• C12N 15/53 - Oxydoréductases (1)
• C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine

Abrégé

Provided are a recombinant microorganism for producing L-valine, a construction method therefor and the use thereof. By means of enhancing the activity of amino acid dehydrogenase in L-valine fermentation strains, and/or activating the Entner-Doudoroff (ED) pathway, the problem of the reducing power in the fermentation process of L-valine being unbalanced is solved, thereby improving the yield and conversion rate of L-valine produced by using Escherichia coli and realizing the one-step anaerobic fermentation of L-valine.

Classes IPC  ?

• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine
• C12N 15/53 - Oxydoréductases (1)
• C12R 1/19 - Escherichia coli

Abrégé

A recombinant microorganism for producing L-valine, a construction method therefor, and an application thereof. By introducing an acetohydroxy acid reductoisomerase gene and/or an amino acid dehydrogenase gene into a microorganism, and improving the activity of acetohydroxy acid reductoisomerase and the activity of amino acid dehydrogenase, the yield and conversion rate of L-valine produced by Escherichia coli can be increased, and one-step anaerobic fermentation of L-valine can be achieved.

Classes IPC  ?

• C12N 15/74 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine
• C12R 1/13 - Brevibacterium

Abrégé

A method for preparing glucosamine includes the steps of converting fructose-6-phosphate (F6P) and an ammonium salt to glucosamine-6-phosphate (GlcN6P) under the catalysis of glucosamine-6-phosphate deaminase (EC 3.5.99.6, GlmD); and producing glucosamine (GlcN) by the dephosphorylation of GlcN6P under the catalysis of an enzyme capable of catalyzing the dephosphorylation. Such a method can be used to prepare glucosamine by in vitro enzymatic biosystem.

Classes IPC  ?

• C12P 19/26 - Préparation d'hydrates de carbone contenant de l'azote
• C12P 19/28 - N-glucosides

Abrégé

Disclosed in the present invention are a formaldehyde conversion mutant protein and an application thereof. The mutant protein can catalyze preparation of 1,3-dihydroxyacetone from formaldehyde, and further synthesis of lactic acid and glycolic acid by the 1,3-dihydroxyacetone. Also disclosed is a method for synthesis of lactic acid and glycolic acid. The mutant protein in the present invention can improve the catalytic efficiency from formaldehyde to 1,3-dihydroxyacetone, and conditions are mild.

Classes IPC  ?

• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
• C12P 7/24 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carbonyle
• C12P 7/26 - Cétones

Abrégé

Recombinant strains are obtained for the production of allulose, allose, and allitol by regulating intracellular glucose metabolism, reducing the enzyme activity of fructose 6-phosphate kinase, and enhancing the enzyme activities of glucokinase and glucose-6-phosphate isomerase, allulose 6-phosphate 3-epimerase, allulose 6-phosphate phosphatase, fructose permease and fructokinase, and optionally enhancing the enzyme activities of ribose 5-phosphate isomerase, allose 6-phosphate phosphatase, ribitol dehydrogenase, glycerol permease, glycerol dehydrogenase, and dihydroxyacetone kinase. A method for producing allulose and allose is an extracellular multienzyme cascade method. Multienzyme cascade catalysis and fermentation are coupled to improve the conversion rate of starch sugar or sucrose to the synthesized allulose.

Classes IPC  ?

• C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
• C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
• C12N 9/16 - Hydrolases (3.) agissant sur les liaisons esters (3.1)
• C12N 9/90 - Isomérases (5.)
• C12N 9/92 - Isomérase de glucose
• C12P 19/02 - Monosaccharides
• C12P 19/24 - Préparation de composés contenant des radicaux saccharide préparés par action d'une isomérase, p. ex. fructose

Abrégé

Provided are a Corynebacterium glutamicum that produces L-lysine, a method for constructing the L-lysine-producing bacterium, and a method for preparing L-lysine using the bacterium. The lysine yield and glucose conversion rate are improved by using the L-lysine-producing bacterium, thereby reducing production costs.

Classes IPC  ?

• C07K 14/34 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries provenant de Corynebacterium (G)
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium
• C12R 1/15 - Corynebacterium
• C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression

Abrégé

The present disclosure relates to D-xylulose 4-epimerase, a mutant thereof and use thereof. Specifically, the present disclosure relates to a polypeptide having D-xylulose 4-epimerase activity, a method for preparing the polypeptide, and use of the polypeptide in the preparation of L-pentose using D-xylose or D-xylulose as a raw material. Compared with the conventional production method in the prior art, the new method for preparing L-pentose discovered in the present disclosure has a simpler production process and reduces the costs of producing L-pentose.

Classes IPC  ?

• C12N 9/90 - Isomérases (5.)
• C12N 15/61 - Isomérases (5)
• C12P 19/24 - Préparation de composés contenant des radicaux saccharide préparés par action d'une isomérase, p. ex. fructose
• C12P 19/02 - Monosaccharides

Abrégé

A construction method for genetically engineered bacteria of a filamentous fungus. The method comprises: enabling, by a genetic engineering method, a filamentous fungus to overexpress an ethanol synthesis positive regulatory gene, and/or down-regulate an endogenous ethanol synthesis negative regulatory gene; or in a cell of the filamentous fungus, overexpressing acetaldehyde dehydrogenase and alcohol dehydrogenase containing a mitochondrial localization signal sequence, or overexpressing pyruvate decarboxylase and alcohol dehydrogenase containing a mitochondrial localization signal sequence, or overexpressing acetaldehyde dehydrogenase, alcohol dehydrogenase, and pyruvate decarboxylase containing a mitochondrial localization signal sequence. Compared with the original strain, the obtained genetically engineered strain has improved ethanol synthesis capability.

Classes IPC  ?

• C12N 1/15 - ChampignonsLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 15/80 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons

Abrégé

Synthesis and use of a protocatechuic acid-based epoxy resin. Specifically, a protocatechuic acid epoxidized monomer has a structure as shown in the following formula (I). The monomer can be cured to form a protocatechuic acid epoxy resin, thereby being used for preparing aerospace special materials.

Classes IPC  ?

• C08G 59/32 - Composés époxydés contenant au moins trois groupes époxyde
• C07D 301/28 - Condensation d'épihalohydrines ou d'halohydrines avec des composés contenant des atomes d'hydrogène actif par réaction avec des radicaux hydroxyle
• C07D 301/30 - Condensation d'épihalohydrines ou d'halohydrines avec des composés contenant des atomes d'hydrogène actif par réaction avec des radicaux carboxyle
• C07D 303/30 - Éthers de composés polyhydroxylés contenant des cycles oxirane dans lesquels tous les radicaux hydroxyle sont éthérifiés par des composés hydroxylés contenant des cycles oxirane

Abrégé

Provided is an MviN protein mutant, said mutant improving corynebacterium glutamic acid and lysine production capability by means of introducing an amino acid mutation at a set position in an MviN protein amino acid sequence.

Classes IPC  ?

• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12P 13/14 - Acide glutamiqueGlutamine
• C12P 13/08 - LysineAcide diaminopiméliqueThréonineValine
• C12R 1/15 - Corynebacterium

Abrégé

Provided are base editing systems for achieving C to A and C to G base mutation and an application thereof. One of the base editing systems, in which C is mutated to A, comprises cytosine deaminase AID and nCas9 nuclease or comprises cytosine deaminase AID, nCas9 nuclease, and uracil DNA glycosidase. The other base editing system, in which C is mutated to G, comprises cytosine deaminase APOBEC, nCas9 nuclease, and uracil DNA glycosidase. Experiments have shown that a combination of three base editing systems, i.e., C to A, C to T, and A to G, can achieve a mutation of A, T, C, or G to any base in prokaryotes. A combination of three base editing systems, i.e., C to G, C to T, and A to G, can achieve a mutation of A, T, C, or G to any base in eukaryotes.

Classes IPC  ?

• C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
• C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
• C12N 15/90 - Introduction stable d'ADN étranger dans le chromosome
• C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression

Abrégé

Provided is a recombinant yeast expressing germacrene A synthetase or a fusion protein thereof, wherein the fusion protein is germacrene A synthetase and farnesyl pyrophosphate synthase. The recombinant yeast improves the yield of germacrene A, and is suitable for the industrialized production of β-elemene and/or germacrene A.

Classes IPC  ?

• C12N 1/18 - Levure de boulangerieLevure de bière
• C12N 9/10 - Transférases (2.)
• C12N 9/88 - Lyases (4.)
• C12R 1/865 - Saccharomyces cerevisiae

Abrégé

Disclosed are a phosphoketolase with enhanced activity and a use thereof in metabolite production. A protein is provided, being a mutant protein obtained by subjecting a phosphoketolase to any one or more of the following mutations: T at position 2 is mutated to A; I at position 6 is mutated to T; N at position 14 is mutated to D; E at position 20 is mutated to D; T at position 120 is mutated to A, E at position 231 is mutated to K; H at position 260 is mutated to Y; E at position 342 is mutated to K; K at position 397 is mutated to R, D at position 676 is mutated to G, F at position 785 is mutated to L; and W at position 801 is mutated to R. Also disclosed is a use of the mutant protein in metabolite production. Compared to existing phosphoketolases, the mutant protein phosphoketolase provided has markedly enhanced enzyme activity, which can significantly increase the yield of a target metabolite.

Classes IPC  ?

• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
• C12P 9/00 - Préparation de composés organiques contenant un métal ou un atome autre que H, N, C, O, S ou un halogène
• C12P 7/24 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carbonyle
• C12P 7/26 - Cétones
• C12P 19/32 - Nucléotides avec un système cyclique condensé, contenant un cycle à six chaînons, comportant deux atomes d'azote dans le même cycle, p. ex. nucléotides puriques, dinucléotide de la nicotinamide-adénine

Abrégé

Rhizopus.

Classes IPC  ?

• C12P 7/46 - Acides dicarboxyliques ayant au plus quatre atomes de carbone, p. ex. acide fumarique, acide maléique
• C07K 14/37 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de champignons
• C12N 15/80 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons
• C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C12N 9/10 - Transférases (2.)
• C12P 7/44 - Acides polycarboxyliques
• C12P 7/50 - Acides polycarboxyliques avec des groupes cétone, p. ex. acide céto-2 glutarique
• C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
• C12N 1/14 - ChampignonsLeurs milieux de culture
• C12R 1/645 - Champignons

Abrégé

A glutamic acid fermentation process, comprising the following steps: introducing Corynebacterium glutamicum into a fermentation tank filled with a clean fermentation medium for fermentation culture, performing ultrasonic treatment, and adjusting the pH value of the fermentation broth.

Classes IPC  ?

• C12P 13/18 - Acide glutamiqueGlutamine utilisant la biotine ou ses dérivés
• C12R 1/15 - Corynebacterium

Abrégé

The present invention provides a class of new mutant proteins for increasing malic acid yield. Specifically, the present invention provides a class of new pyruvate carboxylase mutant protein and malic acid transporter mutant proteins or combinations thereof, a preparation method therefor and use thereof in improving malic acid yield.

Classes IPC  ?

• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C12P 7/46 - Acides dicarboxyliques ayant au plus quatre atomes de carbone, p. ex. acide fumarique, acide maléique

Abrégé

An enzyme synthesizes hydroxyl acetaldehyde and/or 1,3-dihydroxyacetone by catalyzing formaldehyde. Site-directed mutation of benzoylformate decarboxylase (BFD) creates a mutant of the enzyme, which can polymerize the formaldehyde, A phosphoketalose (F/XPK) generates acetyl phosphoric acid from the hydroxyl acetaldehyde or 1,3-dihydroxyacetone (DHA). Combination with phosphotransacetylase (Pta) provides a route from the formaldehyde to acetyl coenzyme A in three steps.

Classes IPC  ?

• C12N 9/88 - Lyases (4.)
• C12P 7/24 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carbonyle
• C12P 7/28 - Produits contenant de l'acétone
• C12P 9/00 - Préparation de composés organiques contenant un métal ou un atome autre que H, N, C, O, S ou un halogène
• C12P 19/32 - Nucléotides avec un système cyclique condensé, contenant un cycle à six chaînons, comportant deux atomes d'azote dans le même cycle, p. ex. nucléotides puriques, dinucléotide de la nicotinamide-adénine

Abrégé

The present invention belongs to the field of enzyme-catalyzed preparation of glucosamine. Provided is a method for preparing glucosamine, especially a method for preparing glucosamine by in vitro enzyme catalysis. The method comprises: catalyzing the conversion of fructose-6-phosphate (F6P) and an ammonium salt into glucosamine-6-phosphate (GlcN6P) using glucosamine-6-phosphate deaminase (EC 3.5.99.6, GlmD); and catalyzing the dephosphorylation of GlcN6P using an enzyme capable of catalyzing the dephosphorylation to produce glucosamine (GlcN).

Classes IPC  ?

• C12P 19/26 - Préparation d'hydrates de carbone contenant de l'azote
• C12N 9/90 - Isomérases (5.)
• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes

Abrégé

Provided are a carbonyl reductase mutant protein and the use thereof in reduction of a cyclopentanedione compound. The mutant protein is a non-native protein, and can be used to catalyze a cyclopentanedione compound to generate a cyclopentanone alcohol. The mutant protein is mutated in four or more core amino acids, related to the enzyme catalytic activity, of a wild type carbonyl reductase.

Classes IPC  ?

• C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
• C12N 15/53 - Oxydoréductases (1)
• C12P 7/26 - Cétones

Abrégé

A construction method and application of a recombinant strain for producing psicose and derivatives thereof. A recombinant strain and synthesized psicose, allose, and allitol are obtained by regulating the intracellular metabolism of glucose, decreasing the enzyme activity of fructose 6-phosphate kinase, and improving the enzyme activity of glucokinase and glucose-6-phosphate isomerase, the enzyme activity of 6-phosphate psicose 3-epimerase, 6-phosphate psicose phosphatase, fructose permease, and fructokinase, and any one of ribose-5-phosphate isomerase, 6-phosphate allose phosphatase, and ribitol dehydrogenase, and the enzyme activity of glycerol permease, glycerol dehydrogenase, and dihydroxyacetone kinase. A method for synthesizing psicose and allose by an extracellular multienzyme cascade method further couples a multienzyme cascade method to a fermentation method to improve the conversion rate that the starch sugar and sucrose are converted into the synthesized psicose. The method has a high conversion rate, is low in cost, and is suitable for producing synthesized psicose and derivates thereof in scale.

Classes IPC  ?

• C12N 15/09 - Technologie d'ADN recombinant
• C12N 1/20 - BactériesLeurs milieux de culture
• C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
• C12P 19/24 - Préparation de composés contenant des radicaux saccharide préparés par action d'une isomérase, p. ex. fructose

Abrégé

Provided is a new use of an influenza virus antibody, in particular the use of an influenza virus antibody for preparing a drug against an H7 subtype influenza virus, wherein the light chain variable region of the influenza virus antibody has the amino acid sequence as set forth in SEQ ID NO.1; and the heavy chain variable region of the influenza virus antibody has the amino acid sequence as set forth in SEQ ID NO.2. The influenza virus antibody can bind well to a HA protein of the H7 subtype of the influenza virus, and especially has a good neutralization effect on the H7N9 influenza virus in vivo, and can prevent the infection with the H7N9 subtype influenza virus that leads to the death of mice.

Classes IPC  ?

• C07K 16/10 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant de virus de virus à ARN
• C12N 15/13 - Immunoglobulines
• C12N 15/85 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules animales
• A61K 39/42 - AnticorpsImmunoglobulinesImmunsérum, p. ex. sérum antilymphocitaire viraux
• A61P 31/16 - Antiviraux pour le traitement des virus ARN de la grippe ou des rhinovirus

Abrégé

An inositol preparation method by enzymatic catalysis uses starch and cellulose or substrates thereof as substrates. Raw materials are converted to inositol by in vitro multi-enzyme reaction system in one pot. The yield from the substrate to inositol is significantly improved by process optimization and adding new enzymes. The new enzymes can promote the phosphorolysis of starch or cellulose and utilization of glucose, which is the final production after the phosphorolysis of starch and cellulose. The inositol preparation method described herein has great potentials in industrial production of inositol because of high inositol yield, easy scale-up, low production cost, and lower impact to environment.

Classes IPC  ?

• C12P 7/02 - Préparation de composés organiques contenant de l'oxygène contenant un groupe hydroxyle
• C12N 9/42 - Hydrolases (3.) agissant sur les composés glycosyliques (3.2) agissant sur les liaisons bêta-glucosidiques-1, 4, p. ex. cellulase

Abrégé

121212 by using compounds as raw materials.

Classes IPC  ?

• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
• C12P 19/42 - Cobalamines, c.-à-d. vitamines B12, facteur LLD
• C12R 1/19 - Escherichia coli

Abrégé

Disclosed is a new tagatose-6-phosphate 4-epimerase, which is capable of converting fructose-6-phosphate into tagatose-6-phosphate and vice versa. Also disclosed is an application of the enzyme in tagatose production.

Classes IPC  ?

• C12N 9/90 - Isomérases (5.)
• C12N 15/61 - Isomérases (5)
• C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12P 19/24 - Préparation de composés contenant des radicaux saccharide préparés par action d'une isomérase, p. ex. fructose

Abrégé

The present invention is in the field of bioelectricity. The present invention provides energy generating systems, methods, and devices that are capable of converting chemical energy stored in sugars into useful electricity.

Classes IPC  ?

• H01M 8/16 - Éléments à combustible biochimique, c.-à-d. éléments dans lesquels des micro-organismes agissent comme catalyseurs

Abrégé

Disclosed in the present invention are an enzyme for synthesizing hydroxyl acetaldehyde and/or 1,3-dihydroxyacetone by catalyzing formaldehyde, and applications thereof. In the present invention, by means of site-directed mutation of BFD, a mutant of the enzyme is found; and by means of the mutant of the enzyme, polymerization of the formaldehyde is implemented; in addition, by means of F/XPK, generation of acetyl phosphoric acid from the hydroxyl acetaldehyde or 1,3-dihydroxyacetone is implemented; with combination of phosphotransacetylase (Pta), a route from the formaldehyde to acetyl coenzyme A is achieved in three steps with the enzyme.

Classes IPC  ?

• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
• C12P 9/00 - Préparation de composés organiques contenant un métal ou un atome autre que H, N, C, O, S ou un halogène
• C12P 7/24 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carbonyle
• C12P 7/26 - Cétones
• C12P 19/32 - Nucléotides avec un système cyclique condensé, contenant un cycle à six chaînons, comportant deux atomes d'azote dans le même cycle, p. ex. nucléotides puriques, dinucléotide de la nicotinamide-adénine

Abrégé

Disclosed is a solid state biological reaction device, comprising a main tank (1), wherein the device further comprises a support (2) supporting the main tank (1), and the support (2) makes the main tank (1) be rotational in the horizontal position, and be statically cultured in the vertical position. The device is relatively simple, in particular, the mixing of materials uses the method of a vehicle-tank in combination with rotation, achieving the tank free conversion between the two different poses of vertical and the horizontal; and the device conducts the work of loading, inoculation, cultivation and transplantation and so on in the upright pose, and completes the work of sterilization and mixing of materials and so on in the horizontal pose. The device is not only quick to use and easy to move, but also omits the stirring system which occupies a lot of manufacturing costs, and is easy to use in large-scale production.

Classes IPC  ?

• C12M 1/16 - Appareillage pour l'enzymologie ou la microbiologie contenant ou adaptés pour contenir des milieux solides
• C12M 1/04 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz
• C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
• C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
• C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
• C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
• C12N 1/14 - ChampignonsLeurs milieux de culture
• C12N 1/18 - Levure de boulangerieLevure de bière

Abrégé

rhizopus.

Classes IPC  ?

• C12P 7/46 - Acides dicarboxyliques ayant au plus quatre atomes de carbone, p. ex. acide fumarique, acide maléique
• C07K 14/37 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de champignons
• C12N 15/80 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons
• C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C12N 9/10 - Transférases (2.)
• C12P 7/44 - Acides polycarboxyliques
• C12P 7/50 - Acides polycarboxyliques avec des groupes cétone, p. ex. acide céto-2 glutarique
• C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
• C12R 1/645 - Champignons

Abrégé

Disclosed is a new approach for synthesizing an acetyl coenzyme A and a derivative product thereof (5-phosphoarabinose, acetyl phosphoric acid, acetyl phosphate, and an acetyl coenzyme A derivative compound) using glycolaldehyde. The approach comprises a reaction of glycolaldehyde and 3-phosphoglyceraldehyde under the catalysis of an enzyme to generate 5-phosphoarabinose, wherein the enzyme is selected from an aldolase, a transaldolase, an isoenzyme and a mutant enzyme thereof.

Classes IPC  ?

• C12P 19/02 - Monosaccharides
• C12P 9/00 - Préparation de composés organiques contenant un métal ou un atome autre que H, N, C, O, S ou un halogène
• C12P 19/32 - Nucléotides avec un système cyclique condensé, contenant un cycle à six chaînons, comportant deux atomes d'azote dans le même cycle, p. ex. nucléotides puriques, dinucléotide de la nicotinamide-adénine
• C12P 7/04 - Préparation de composés organiques contenant de l'oxygène contenant un groupe hydroxyle acycliques

Abrégé

Provided is a recombinant yeast expressing germacrene A synthetase or a fusion protein thereof, wherein the fusion protein is germacrene A synthetase and farnesyl pyrophosphate synthase. The recombinant yeast improves the yield of germacrene A, and is suitable for the industrialized production of β-elemene and/or germacrene A.

Classes IPC  ?

• C12N 15/81 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons pour levures
• C12N 1/16 - LevuresLeurs milieux de culture
• C12R 1/85 - Saccharomyces

Abrégé

The present invention provides a polypeptide complex on the basis of Titin-Telethonin beta-pleated sheet structure as a polypeptide or protein drug carrier, a method of using the polypeptide complex, and a fusion protein complex thereof. The polypeptide complex is capable of maintaining the activity of polypeptide or protein drugs and prolonging the half-life period simultaneously.

Classes IPC  ?

• C07K 19/00 - Peptides hybrides
• C07K 14/47 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'animauxPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'humains provenant de vertébrés provenant de mammifères
• C07K 14/605 - Glucagons
• C07K 14/655 - Somatostatines
• A61K 47/62 - Préparations médicinales caractérisées par les ingrédients non actifs utilisés, p. ex. les supports ou les additifs inertesAgents de ciblage ou de modification chimiquement liés à l’ingrédient actif l’ingrédient non actif étant chimiquement lié à l’ingrédient actif, p. ex. conjugués polymère-médicament l’ingrédient non actif étant un agent de modification l’agent de modification étant une protéine, un peptide ou un acide polyaminé
• A61K 38/00 - Préparations médicinales contenant des peptides
• A61P 3/10 - Médicaments pour le traitement des troubles du métabolisme de l'homéostase du glucose de l'hyperglycémie, p. ex. antidiabétiques
• A61K 47/42 - ProtéinesPolypeptidesLeurs produits de dégradationLeurs dérivés p. ex. albumine, gélatine ou zéine
• A61K 9/00 - Préparations médicinales caractérisées par un aspect particulier
• A61K 38/26 - Glucagons
• A61K 38/22 - Hormones
• A61K 38/31 - Somatostatines
• C07K 14/78 - Peptides du tissu connectif, p. ex. collagène, élastine, laminine, fibronectine, vitronectine ou globuline insoluble à froid [CIG]

Abrégé

Disclosed is a solid state biological reaction device, comprising a main tank (1), wherein the device further comprises a support (2) supporting the main tank (1), and the support (2) makes the main tank (1) be rotational in the horizontal position, and be statically cultured in the vertical position. The device is relatively simple, in particular, the mixing of materials uses the method of a vehicle-tank in combination with rotation, achieving the tank free conversion between the two different poses of vertical and the horizontal; and the device conducts the work of loading, inoculation, cultivation and transplantation and so on in the upright pose, and completes the work of sterilization and mixing of materials and so on in the horizontal pose. The device is not only quick to use and easy to move, but also omits the stirring system which occupies a lot of manufacturing costs, and is easy to use in large-scale production.

Classes IPC  ?

• C12M 1/16 - Appareillage pour l'enzymologie ou la microbiologie contenant ou adaptés pour contenir des milieux solides
• C12M 1/04 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz
• C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture

Abrégé

Disclosed is a preparation containing ergothioneine. The ergothioneine is provided by an extracellular ferment liquor and/or the concentration of the extracellular ferment liquor which is obtained by removing the mycelia from the ferment liquor produced through the liquid fementation of the mushroom, and the preparation comprises food, cosmetics and animal feed. The method for preparing the preparation comprises liquid-fermentation of the mushroom, removing the mycelia from the fermentation product, and selectively concentrating the extracellular ferment liquor obtained after removing the mycelia. The extracellular ferment liquor and/or the concentration of the extracellular ferment liquor obtained through the liquid fermentation of the mushroom are used in preparing the preparation containing ergothioneine.

Classes IPC  ?

• A61K 8/44 - Acides aminocarboxyliques ou leurs dérivés, p. ex. acides aminocarboxyliques contenant du soufreLeurs sels, esters ou dérivés N-acylés
• C12P 13/04 - Alpha- ou bêta-amino-acides

Abrégé

Provided is an inositol preparation method. By using starch and cellulose or substrates thereof as substrates, adding an enzyme capable of promoting hydrolysis of the starch or the cellulose and by using an enzyme of a by-product glucose, a multienzyme reaction system is established, and the substrates are converted into inositol. The conversion rates of raw materials and the yield of the inositol are improved.

Classes IPC  ?

• C12P 7/02 - Préparation de composés organiques contenant de l'oxygène contenant un groupe hydroxyle

Abrégé

Provided are an engineering strain for synthesizing a dibasic organic acid and preparation and application of same. The engineering strain introduces or up-regulates expression of a positive regulator gene for synthesis of a dibasic organic acid, and/or down-regulates expression of a negative regulator gene for synthesis of a dibasic organic acid; as compared with the origin strain of the engineering strain, the producing capability for producing the dibasic organic acid is improved. The dibasic organic acid comprises malic acid, succinic acid, fumaric acid, oxaloacetic acid, glutaric acid, and adipic acid; the expression product of the positive regulator gene comprises aspartate transaminase, glutamic-aspartate transport protein, C4-dicarboxylic transport protein, pyruvate carboxylase, malate dehydrogenase, and glucose transport protein; the expression product of the negative regulator gene comprises succinyl coenzyme A synthase and malic-α-ketoglutaric transport protein; and the origin strain comprises myceliophthora thermophila, thielavia terrestris, aspergillus, and rhizopus.

Classes IPC  ?

• C12N 1/15 - ChampignonsLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 15/80 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons
• C12P 7/50 - Acides polycarboxyliques avec des groupes cétone, p. ex. acide céto-2 glutarique
• C12P 7/46 - Acides dicarboxyliques ayant au plus quatre atomes de carbone, p. ex. acide fumarique, acide maléique
• C12P 7/44 - Acides polycarboxyliques
• C12N 9/10 - Transférases (2.)
• C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C07K 14/37 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de champignons
• C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
• C12R 1/645 - Champignons