The disclosure provides binding agents (e.g., antibodies) against isoform 1 of human ADAM9, as well as kits and methods for using the same (e.g., immunoassays) as part of a companion diagnostic and for other applications. In some aspects, the binding agents described herein may be used in assays for detecting non-small cell lung cancer (NSCLC), pancreatic cancer, triple-negative breast cancer (TNBC), gastroesophageal cancer (GC), prostate cancer, and renal cancer.
C07K 16/38 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against protease inhibitors of peptide structure
C12Q 1/37 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase involving peptidase or proteinase
G01N 33/573 - ImmunoassayBiospecific binding assayMaterials therefor for enzymes or isoenzymes
2.
SYSTEMS AND METHODS FOR A VENTLESS GAS CHROMATOGRAPHY MASS SPECTROMETRY INTERFACE
Systems and methods for a ventless GC-MS interface are described herein. The system can include a gas chromatograph. The system can include a mass spectrometer connected to the gas chromatograph by a mass spectrometer flow path. The system can include a fitting. The fitting can include a sealing surface and one or more gas purge flow paths. The fitting can be fluidically connected to the mass spectrometer. The system can include a column disposed in a ferrule.
H01J 49/04 - Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locksArrangements for external adjustment of electron- or ion-optical components
The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
Systems and methods for a ventless GC-MS interface are described herein. The system can include a gas chromatograph. The system can include a mass spectrometer connected to the gas chromatograph by a mass spectrometer flow path. The system can include a fitting. The fitting can include a sealing surface and one or more gas purge flow paths. The fitting can be fluidically connected to the mass spectrometer. The system can include a column disposed in a ferrule.
G01N 30/32 - Control of physical parameters of the fluid carrier of pressure or speed
H01J 49/04 - Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locksArrangements for external adjustment of electron- or ion-optical components
5.
CAPILLARY CONDITIONING FOR PROTEIN ELECTROPHORESIS
A capillary conditioning solution is formulated for coating an inside surface of a capillary utilized for capillary electrophoresis (CE). The solution may be utilized to condition the capillary before a CE run, and may be applied to the capillary between one or more different CE runs to maintain the effectiveness of the conditioning.
In some examples, an apparatus may include an ion source including at least one lens that is partitioned by at least one partition into at least two lens partitions. The at least two lens partitions may be connectable to a direct current (DC) bias power supply to bias the at least two lens partitions and a radio frequency alternating current (RF AC) voltage power supply to produce a dipolar RF field within the at least one lens.
In some examples, an apparatus may include an ion source including at least one lens that is partitioned by at least one partition into at least two lens partitions. The at least two lens partitions may be connectable to a direct current (DC) bias power supply to bias the at least two lens partitions and a radio frequency alternating current (RF AC) voltage power supply to produce a dipolar RF field within the at least one lens.
Apparatus and methods for automated transfer of slides into and/or out of a slide basket. One of the slides is raised above other slides in the slide basket, and a suction member on a slide gripper contacts the raised slide. A vacuum is drawn in the suction member sufficient for the suction member to grip the raised slide, and the slide gripper moves to withdraw the raised slide from the slot of the slide basket. The slide gripper moves the gripped slide to a slide processing module or other desired location.
09 - Scientific and electric apparatus and instruments
Goods & Services
Mass detection systems; instruments for mass spectrometry
measurements; liquid chromatography mass spectrometry
systems; liquid chromatography mass detection systems;
scientific instruments, namely, liquid chromatography mass
spectrometers, including liquid delivery system (pump),
injection system, autosampler, column compartment, valving,
ion source generation, and calibrant delivery systems;
software for instrument control, instrument tuning, signal
collection, data analysis, and reporting of the output of
liquid chromatography mass spectrometers; liquid
chromatography mass spectrometry detection systems for
biological and chemical separations, measurements,
screening, targeted analysis, and identification.
09 - Scientific and electric apparatus and instruments
Goods & Services
Mass detection systems; instruments for mass spectrometry
measurements; liquid chromatography mass spectrometry
systems; liquid chromatography mass detection systems;
scientific instruments, namely, liquid chromatography mass
spectrometers, including liquid delivery system (pump),
injection system, autosampler, column compartment, valving,
ion source generation, and calibrant delivery systems;
software for instrument control, instrument tuning, signal
collection, data analysis, and reporting of the output of
liquid chromatography mass spectrometers; liquid
chromatography mass spectrometry detection systems for
biological and chemical separations, measurements,
screening, targeted analysis, and identification.
13.
DECONVOLUTION BY VISUAL ACUITY-BASED INTERPRETATION OF MASS SPECTROMETRY (MS) AND TANDEM MASS SPECTROMETRY (MS/MS) SPECTRA
In some examples, deconvolution by visual acuity-based interpretation of mass spectrometry (MS) and tandem mass spectrometry (MS/MS) spectra may include receiving, for an ion that is be identified, a plurality of expected ion spectra, where each expected ion spectrum of the plurality of expected ion spectra may include at least one expected peak profile, receiving an observed ion spectrum including at least one observed peak profile, and identifying characteristics of the expected ion spectra and the observed ion spectrum. The characteristics may be analyzed to determine ion scores, where a highest ion score may be used to identify a corresponding expected ion spectrum. An indication and/or a graphical user interface display of the expected ion spectrum corresponding to the highest ion score may be generated.
A configurable injector for injecting a fluidic sample in a separation path of a sample separation apparatus includes a sample accommodation volume for accommodating the fluidic sample to be injected into the separation path, a valve arrangement fluidically couplable with the separation path, fluidically coupled with the sample accommodation volume, and being controllable for injecting the fluidic sample into the separation path, an input interface configured for receiving input data indicative of an injection profile of an injector to be emulated by the configurable injector, and a control unit configured for controlling the configurable injector, in particular the valve arrangement, so that the configurable injector is operated in accordance with the injection profile to thereby emulate the injector to be emulated.
F16K 11/074 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with all movable sealing faces moving as one unit comprising only sliding valves with pivoted closure members with flat sealing faces
Methods and systems for estimating gas supply pressure are described herein. The method can include measuring a runtime valve duty cycle for a valve of a gas chromatography system. The method can include estimating a runtime supply pressure from the runtime valve duty cycle and at least one of a runtime flow rate or downstream pressure based on one or more calibration valve duty cycles corresponding to one or more calibration supply pressures and one or more calibration flow rates. The method can include, responsive to determining the runtime supply pressure is greater than a threshold supply pressure value, generating a notification that the runtime supply pressure is greater than the threshold supply pressure value.
G01N 30/32 - Control of physical parameters of the fluid carrier of pressure or speed
B01D 15/16 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
Methods and systems for estimating gas supply pressure are described herein. The method can include measuring a runtime valve duty cycle for a valve of a gas chromatography system. The method can include estimating a runtime supply pressure from the runtime valve duty cycle and at least one of a runtime flow rate or downstream pressure based on one or more calibration valve duty cycles corresponding to one or more calibration supply pressures and one or more calibration flow rates. The method can include, responsive to determining the runtime supply pressure is greater than a threshold supply pressure value, generating a notification that the runtime supply pressure is greater than the threshold supply pressure value.
A column including particles having an average particle size ranging from about 1 μm to about 5 μm; the particles have an average pore size ranging from about 450 Å to about 3000 Å; and the particles have an average pore volume ranging from about 0.1 cm3/g to about 5 cm3/g is disclosed. The column can be a size exclusion chromatography column. The column can be used in a method to separate monomers from viral analytes, which is also disclosed.
A column including particles having an average particle size ranging from about 1 μm to about 5 μm; the particles have an average pore size ranging from about 450 Å to about 3000 Å; and the particles have an average pore volume ranging from about 0.1 cm3/g to about 5 cm3/g is disclosed. The column can be a size exclusion chromatography column. The column can be used in a method to separate monomers from viral analytes, which is also disclosed.
B01D 15/20 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
B01D 15/34 - Size-selective separation, e.g. size-exclusion chromatographyGel filtrationPermeation
B01J 20/28 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof characterised by their form or physical properties
09 - Scientific and electric apparatus and instruments
Goods & Services
Chromatography apparatus for laboratory use; Measuring apparatus; Measuring instruments; Testing apparatus not for medical purposes; Material testing instruments and machines; Liquid chromatography equipment comprising pumps, injectors, autosamplers, column-compartments and columns, detectors, valves and valve-actuators; Laboratory operating systems comprising computer hardware and computer software for use in connection with liquid chromatography systems; Liquid chromatographs for laboratory use; Detectors.
A composition, including an alkaloid, and a diaryl ketone is disclosed. Also, disclosed is a kit including one or more containers in which each of the one or more containers includes the composition. A method of using a standard composition is also disclosed.
A system for electronically and optically monitoring biological samples, the system including: a multi-well plate having a plurality of wells configured to receive a plurality of biological samples, each of the wells having a set of electrodes and a transparent window on a bottom surface of the well that is free of electrodes; an illumination module configured to illuminate the wells; a cradle configured to receive the multi-well plate, the cradle having an opening on the bottom that exposes the transparent windows of the wells; and an optical imaging module movable across different wells of a same multi-well plate to capture images through the windows.
A composition, including an alkaloid, and a diaryl ketone is disclosed. Also, disclosed is a kit including one or more containers in which each of the one or more containers includes the composition. A method of using a standard composition is also disclosed.
In some examples, a system may include at least two conveyors disposed at a specified distance apart and movable toward each other to reduce the specified distance or away from each other to increase the specified distance. The at least two conveyors may be operable to move a container that is contiguously disposable between the at least two conveyors along a first direction when the at least two conveyors are operated in the first direction and along a second direction that is opposite to the first direction when the at least two conveyors are operated in the second direction.
B65G 15/14 - Conveyors having endless load-conveying surfaces, i.e. belts and like continuous members, to which tractive effort is transmitted by means other than endless driving elements of similar configuration comprising two or more co-operating endless surfaces with parallel longitudinal axes, or a multiplicity of parallel elements, e.g. ropes defining an endless surface with two or more endless belts the load being conveyed between the belts
25.
SYSTEM AND METHOD FOR VARIABLE SIZE CONTAINER HANDLING
In some examples, a system may include at least two conveyors disposed at a specified distance apart and movable toward each other to reduce the specified distance or away from each other to increase the specified distance. The at least two conveyors may be operable to move a container that is contiguously disposable between the at least two conveyors along a first direction when the at least two conveyors are operated in the first direction and along a second direction that is opposite to the first direction when the at least two conveyors are operated in the second direction.
B65C 9/04 - Devices for moving articles, e.g. containers, past labelling station having means for rotating the articles
B65C 3/08 - Affixing labels to short rigid containers to container bodies
B65G 15/14 - Conveyors having endless load-conveying surfaces, i.e. belts and like continuous members, to which tractive effort is transmitted by means other than endless driving elements of similar configuration comprising two or more co-operating endless surfaces with parallel longitudinal axes, or a multiplicity of parallel elements, e.g. ropes defining an endless surface with two or more endless belts the load being conveyed between the belts
B65G 21/14 - Supporting or protective framework or housings for endless load-carriers or traction elements of belt or chain conveyors movable, or having interchangeable or relatively- movable partsDevices for moving framework or parts thereof to allow adjustment of length or configuration of load-carrier or traction element
Methods and compositions are used for evaluating the performance of a mass spectrometer instrument. An oligonucleotide is introduced to the mass spectrometry instrument, the sample is analyzed in negative ionization mode, and the resulting mass spectrometry peaks are compared to known standards of the nucleotide-based sample for purposes of performing a qualification of the mass spectroscopy instrument in negative ionization mode. Compositions are provided for the methods and systems.
In some examples, an apparatus may include an ion injector including an ion injector entrance and an ion injector exit, and a capillary cap disposed at the ion injector exit. The capillary cap may include a capillary cap exit opening that is shaped and sized to reduce gas turbulence after the ion injector exit and improve ion signal stability.
H01J 37/317 - Electron-beam or ion-beam tubes for localised treatment of objects for changing properties of the objects or for applying thin layers thereon, e.g. ion implantation
The disclosure provides methods for aligning a portion of a first image of a tissue with a portion of a second image of the tissue by obtaining one or more local transformations based on the portion of the first image and/or the portion of the second image, wherein each of the one or more local transformations is: (i) for aligning a patch of the first image to a patch of the second image matching the patch of the first image, and (ii) associated with the patch of the first image and/or with the patch of a second image; and determining a transformation for aligning the portion of the first image to the portion of the second image according to the one or more local transformations. Moreover, a corresponding device and software implementing functionality of the methods is provided.
In alternative embodiments, provided are non-natural synthetic antibodies capable of specifically binding a human CD10 polypeptide, or neprilysin, polypeptide. In alternative embodiments, also provided are products of manufacture and kits comprising antibodies as provided herein, and methods for making and using antibodies as provided herein, where the antibodies can be used for in vitro diagnostics by immunohistochemistry (IHC). In alternative embodiments, antibodies as provided herein are used in IHC protocols to diagnose a cancer, for example, leukemic cell cancer of pre-B phenotype, acute lymphoblastic leukemia (ALL), angioimmunoblastic T cell lymphoma, Burkitt lymphoma, chronic myelogenous leukemia in blast crisis, diffuse large B-cell lymphoma, hairy cell leukemia, myeloma, precursor B lymphoblastic leukemia or lymphoma, follicular lymphoma, mantle cell lymphoma, precursor T lymphoblastic leukemia or lymphoma, non-hematolymphoid sarcoma, or carcinoma such as renal cell carcinoma or metaplastic breast carcinoma.
G01N 33/574 - ImmunoassayBiospecific binding assayMaterials therefor for cancer
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
09 - Scientific and electric apparatus and instruments
Goods & Services
Mass detection systems; instruments for mass spectrometry measurements; liquid chromatography mass spectrometry systems; liquid chromatography mass detection systems; scientific instruments, namely, liquid chromatography mass spectrometers, including liquid delivery system (pump), injection system, autosampler, column compartment, valving, ion source generation, and calibrant delivery systems; software for instrument control, instrument tuning, signal collection, data analysis, and reporting of the output of liquid chromatography mass spectrometers; liquid chromatography mass spectrometry detection systems for biological and chemical separations, measurements, screening, targeted analysis, and identification
09 - Scientific and electric apparatus and instruments
Goods & Services
Mass detection systems; instruments for mass spectrometry measurements; liquid chromatography mass spectrometry systems; liquid chromatography mass detection systems; scientific instruments, namely, liquid chromatography mass spectrometers, including liquid delivery system (pump), injection system, autosampler, column compartment, valving, ion source generation, and calibrant delivery systems; software for instrument control, instrument tuning, signal collection, data analysis, and reporting of the output of liquid chromatography mass spectrometers; liquid chromatography mass spectrometry detection systems for biological and chemical separations, measurements, screening, targeted analysis, and identification
The invention relates to a fluid valve (100) for an analyzer (10), comprising: i) a stator component (110) comprising: a first port (150) in a first radius (111) and a plurality of second ports (114) along a second radius (112); and ii) a rotor component (120) comprising: a slot (121) which is coupled to the first port (150), wherein the slot (121) comprises: a tangential part (122) which extends along a segment of the second radius (112). a) the slot (121) also comprises a radial part (125) which extends from the first port (150) to the second radius (112), and/or b) the fluid valve (100) is designed to rotate the rotor component (120) in relation to the stator component (110) in such a way that mixing and/or proportioning fluid, in particular solvent (113, 115), is enabled.
G01N 30/34 - Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
F16K 11/074 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with all movable sealing faces moving as one unit comprising only sliding valves with pivoted closure members with flat sealing faces
33.
SAMPLE INJECTOR WITH FLOATING NEEDLE SEAT FOR AN ANALYTICAL DEVICE
A sample injector for an analytical device includes a needle seat configured to receive a needle for injecting a sample into an injector path. The needle seat is mounted in a floating manner so that the orientation of the needle seat is aligned automatically with respect to the orientation of the approaching needle. The sample injector may be provided in an analytical device such as a chromatographic device.
An apparatus for containing a three-dimensional cellular material surrounded by a medium that facilitates and maintains the centering of the three-dimensional cellular material throughout an assay is provided. The apparatus includes a well having an open proximal end and a closed distal end. Further, the well defines a compartment having an interior surface and a sample nesting site for containing the three-dimensional cellular material surrounded by the medium. A central indentation is located at the closed distal end of the well, a first concentric lip is located above the central indentation in a y-direction towards the open proximal end of the well, and a second concentric lip is located above the first concentric lip in the y-direction towards the open proximal end of the well. Additionally, the first concentric lip and the second concentric lip define a groove therebetween. A method of forming a three-dimensional cellular material is also provided.
A magnetic coupling assembly includes a thermal isolation system that isolates the assembly from a heat source such as a pump coupled to a driven side of the assembly, thereby enabling the assembly to operate at a reduced temperature. The system may include a labyrinth seal that reduces convective heat transfer to the assembly. The seal may include a sealing gap defined between a rotating component and a stationary component. The gap is sized to limit gas conductance therethrough. The system may also include one or more spacers positioned to reduce or eliminate conductive heat transfer from one component to another. The assembly may be utilized in a pump for contactless coupling of a motor shaft to a pump shaft.
H02K 49/10 - Dynamo-electric clutchesDynamo-electric brakes of the permanent-magnet type
F04C 2/02 - Rotary-piston machines or pumps of arcuate-engagement type, i.e. with circular translatory movement of co-operating members, each member having the same number of teeth or tooth-equivalents
F04C 18/02 - Rotary-piston pumps specially adapted for elastic fluids of arcuate-engagement type, i.e. with circular translatory movement of co-operating members, each member having the same number of teeth or tooth-equivalents
F04C 29/00 - Component parts, details, or accessories, of pumps or pumping installations specially adapted for elastic fluids, not provided for in groups
H02K 5/18 - Casings or enclosures characterised by the shape, form or construction thereof with ribs or fins for improving heat transfer
36.
Chemically Modified Guide RNAs for CRISPR/CAS-Mediated Gene Correction
The Board of Trustees of the Leland Stanford Junior University (USA)
Agilent Technologies, Inc. (USA)
Inventor
Porteus, Matthew H.
Hendel, Ayal
Clark, Joe
Bak, Rasmus O.
Ryan, Daniel E.
Dellinger, Douglas J.
Kaiser, Robert
Myerson, Joel
Abstract
Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a modified sgRNA to correct a mutation in a target gene associated with the genetic disease.
A capillary array assembly is fabricated by inserting capillaries into respective grooves of a substrate and fixing the capillaries to the substrate. The capillaries are fixed by applying heat and force to the substrate, with a defined displacement of substrate material and for a defined period of time, thereby spreading heated portions of the substrate over the capillaries. After applying the heat and force, capillaries are at least partially embedded by the substrate and may be at least partially exposed to a top outside surface of the substrate by respective slots. The capillary array assembly may be utilized, for example, in a sample analysis instrument such as a capillary electrophoresis (CE) instrument.
The invention relates to a temperature control apparatus (100) for an analysis device (10), having: i) a receiving volume (110) for receiving at least one sample container (130); and ii) a temperature control element (120) which is designed to at least partially control the temperature of the at least one sample container (130) in the receiving volume (110); wherein the temperature control apparatus (100) is designed to switch between: a) a first operating mode for controlling the temperature of the at least one sample container (130) by means of the temperature control element (120), wherein the temperature control element (120) is designed to directly contact the at least one sample container (130) in the first operating mode, wherein the direct contact comprises: forming an integral connection and/or controlling the temperature by means of a temperature control medium (125) which flows through the temperature control element (120); b) a second operating mode for not controlling the temperature of the at least one sample container (130) by means of the temperature control element (120).
In some examples, a three-dimensional printed nanospray interface for mass spectrometry may include a body including a sheath inlet connected to a sheath outlet for passage of sheath liquid to an emitter. The emitter may include a sidewall including a stepped texture on an inner surface of the sidewall.
In some examples, a three-dimensional printed nanospray interface for mass spectrometry may include a body including a sheath inlet connected to a sheath outlet for passage of sheath liquid to an emitter. The emitter may include a sidewall including a stepped texture on an inner surface of the sidewall.
H01J 49/04 - Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locksArrangements for external adjustment of electron- or ion-optical components
B33Y 80/00 - Products made by additive manufacturing
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
Goods & Services
Custom manufacturing services for others in the field of
drugs for therapeutic use in humans, nucleic acids, and
nucleic acid materials; consulting for custom manufacturing
process; technical support services, namely, providing
technical advice related to the custom manufacture of drugs
for therapeutic use in humans, nucleic acids, and nucleic
acid materials. Pharmaceutical drug development services; biochemical
research and analysis, namely, analysis of nucleic acids;
scientific study and research in the field of nucleic acids
and nucleic acid materials; quality management services,
namely, quality evaluation and analysis, quality assurance,
and quality control, in the field of pharmaceutical drugs
for therapeutic use in humans.
42.
IMMUNOHISTOCHEMISTRY (IHC) PROTOCOLS AND METHODS FOR DIAGNOSING AND TREATING CANCER - CLAUDIN 18.2
In alternative embodiments, provided are immunohistochemistry (IHC) methods for determining and scoring reproducibly the extent of expression of the protein Claudin 18.2, in a tissue sample. In alternative embodiments, provided are methods for diagnosing, treating or ameliorating or assessing the risk of recurrence for a cancer or a tumor using an IHC method as provided herein. In alternative embodiments, provided are kits comprising components and instructions for practicing methods as provided herein. The present application describes methods for scoring Claudin 18.2 expression and utilizing the score as a companion or complementary diagnostic or to treat or ameliorate cancer or a tumor.
G01N 33/574 - ImmunoassayBiospecific binding assayMaterials therefor for cancer
C07K 16/18 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans
A61K 39/395 - AntibodiesImmunoglobulinsImmune serum, e.g. antilymphocytic serum
C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
Goods & Services
Custom manufacturing services for others in the field of
drugs for therapeutic use in humans, nucleic acids, and
nucleic acid materials; consulting for custom manufacturing
process; technical support services, namely, providing
technical advice related to the custom manufacture of drugs
for therapeutic use in humans, nucleic acids, and nucleic
acid materials. Pharmaceutical drug development services; biochemical
research and analysis, namely, analysis of nucleic acids;
scientific study and research in the field of nucleic acids
and nucleic acid materials; quality management services,
namely, quality evaluation and analysis, quality assurance,
and quality control, in the field of pharmaceutical drugs
for therapeutic use in humans.
In some examples, an apparatus may include an advection flow structure including a passage to transport, via advection by a gas flow, an ablated sample from an ablation region to an ionization region.
H01J 49/04 - Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locksArrangements for external adjustment of electron- or ion-optical components
H01J 49/26 - Mass spectrometers or separator tubes
H01J 49/24 - Vacuum systems, e.g. maintaining desired pressures
The present invention relates to methods for construction of pharmamers i.e. vaccine components characterized by their multimerization domain and the attached biologically active molecules, and their use in preparation of vaccines that contains the pharmamers alone or in combination with other molecules. The individual molecules of the construct can be bound to each other or the multimerization domain(s) by covalent or non-covalent bonds, directly or via linkers. The invention further relates to the use of such preparations in vaccine settings aimed to function as preventive/prophylactic or therapeutic vaccines in humans and animals.
A61K 39/21 - Retroviridae, e.g. equine infectious anemia virus
A61K 47/61 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule the organic macromolecular compound being a polysaccharide or a derivative thereof
A61K 47/64 - Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
C07K 14/74 - Major histocompatibility complex [MHC]
In some examples, an apparatus may include an advection flow structure including a passage to transport, via advection by a gas flow, an ablated sample from an ablation region to an ionization region.
H01J 49/04 - Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locksArrangements for external adjustment of electron- or ion-optical components
H01J 49/16 - Ion sourcesIon guns using surface ionisation, e.g. field-, thermionic- or photo-emission
H01J 49/24 - Vacuum systems, e.g. maintaining desired pressures
The present invention is directed to a sample holder for use in molecular absorption spectroscopy. The sample holder comprises a first surface having a first predetermined geometry. and a second surface having a second predetermined geometry. The first surface is opposite the second surface. The sample holder is configured to hold a measurement sample between the first surface and the second surface such that a distance between the first surface and the second surface defines an optical pathlength of the sample holder. The predetermined geometries of the first surface and the second surface provides a continuously variable cross-section across the sample holder so as to provide a continuous range of optical pathlengths.
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
48.
Smartphone display screen or portion thereof with transitional graphical user interface
09 - Scientific and electric apparatus and instruments
Goods & Services
Computer software, namely, downloadable and recorded
software for collecting, processing, integrating,
organizing, analyzing, visualizing, and communicating
scientific data in the field of mass spectrometry.
Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as a rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.
A cell filtration device and method use a negative dielectrophoretic force (nDEP) to repulse cells from pores of an insulating membrane. A cell suspension is introduced to a first fluidic region, and a fluid and other components pass through pores of the insulating membrane to a second fluidic region. Cells are retained in the first fluidic region and can be recovered continuously or at intervals. The cell filtration device and method minimize contact of the insulating membrane by the cells and avoids clogging of pores.
In alternative embodiments, provided are immunohistochemistry (IHC) methods for determining and scoring reproducibly the extent of expression of the protein Melanoma Associated Antigen Gene-A4 (MAGE-A4), in a tissue sample. In alternative embodiments, provided are methods for diagnosing, treating or ameliorating or assessing the risk of recurrence for a cancer or a tumor using an IHC method as provided herein. In alternative embodiments, provided are kits comprising components and instructions for practicing methods as provided herein. The present application describes methods for scoring MAGE-A4 expression and utilizing the score as a companion or complementary diagnostic or to treat or ameliorate cancer or a tumor.
G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
C07K 16/30 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
A capillary array assembly (100) includes a capillary array window holder (104) that provides multiple capillary channels. A section of each capillary channel is an open channel (678) that is exposed to excitation light on at least one side of the capillary array assembly (100). Adjacent open channels (678) are separated from each other by window bars (156). Multiple capillaries (108) are disposed in respective capillary channels, such that windows (148) of the capillaries (108) are located in the open channels (678). The window bars (156) block line of sight between adjacent capillary windows (148) to reduce or eliminate cross-talk between adjacent capillaries (108) when making optical-based measurements of the samples. The capillary array assembly (100) may be mounted in a sample analysis system (1800), which may for example be configured to perform capillary electrophoresis on the samples.
A solvent supply system supplies solvent for one or more chromatographic separation systems each including one or more input channels. Each input channel is configured for fluidically coupling with a respective solvent container for supplying the respective input channel with a respective solvent from the respective solvent container. The solvent supply system includes a solvent identification unit configured for providing a channel identification for identifying a specific solvent container to supply a specific input channel of a specific chromatographic separation system, and for assigning the channel identification to the specific solvent container.
B01D 15/20 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
B01D 15/18 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
55.
DEPARAFFINIZATION OF TISSUE UTILIZING ELECTRIC FIELD
Paraffin-embedded tissue is prepared removing paraffin from the tissue. The paraffin is removed by generating an electric field effective to produce plasma and direct charged species of the plasma to the paraffin, thereby rendering the paraffin responsive to the electric field. The electric field may move the paraffin out from the tissue due to electrostatic force. Movement of the paraffin may be assisted by moving an electrode utilized to generate the electric field relative to the paraffin. Movement of the paraffin also may be assisted by applying a solvent and/or heat energy to the tissue.
Provided herein is an ion source containing a plurality of components, at least one of which is partially coated with a layer of silicon. The ion source reduces reactivity between the sample and the carrier gas, reduces or eliminates tailing in ion chromatograms, and/or improves mass spectral fidelity. Also provided are methods of using the ion source in a mass spectrometer or gas chromatograph-mass spectrometer.
A flame-based detector (102) comprises a housing (104), a burner (106), a fuel flow path (108), an air flow path (110), a collector (114), an ignitor (116), an ignition promoter port (122), and an exhaust vent (128). The ignition promoter port (122) introduces a gas close to the ignitor (116), thereby facilitating combustion.
In alternative embodiments, provided are immunohistochemistry (IHC) methods and kits for determining and scoring reproducibly the extent of expression of the protein tyrosine-protein kinase-like 7 (PTK7), also known as: colon carcinoma kinase 4 (CCK4); HER2 or receptor tyrosine-protein kinase erbB-2, or cluster of differentiation 340 (CD340); programmed death-ligand 1 (PD-L1), or cluster of differentiation 274 (CD274); B7 homolog 1 (B7-H1); and, Ki-67 or MKI67 (Marker of Proliferation Ki-67), in a tissue sample. In alternative embodiments, provided are methods and kits for diagnosing or selecting an individual eligible for treatment with a cancer or tumor therapeutic, or assessing the risk of recurrence for a cancer or a tumor using an IHC method as provided herein. In alternative embodiments, provided are kits comprising components and instructions for practicing methods as provided herein. The present application describes methods for scoring PTK7 expression and utilizing the score as a companion or complementary diagnostic, or to aid the treatment or amelioration of a cancer or a tumor.
G01N 33/574 - ImmunoassayBiospecific binding assayMaterials therefor for cancer
C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
Flame-based detectors having an ignitor are protected from corrosion by flowing a protective gas over the ignitor. The protective gas layer protects the ignitor from corrosive components in exhaust gas.
The current technology is related to methods for rapidly determining the metabolic baseline and potential of living cells. Embodiments relate to measuring the activity of each of the two major energy-generating pathways within the cell: mitochondrial respiration and glycolysis, first under baseline conditions, and again after applying a stress to the cells to demand increased energy supply. In some embodiments the stress may be applied by exposing the cells to a combination of two chemical compounds: a mitochondrial uncoupler and an ATP synthase inhibitor. In one embodiment, the metabolic energy generating activity of the mitochondrial respiration pathway is determined by measuring the rate of oxygen consumption by the living cells, and the metabolic energy generating activity of the glycolysis pathway is determined from a measurement of extracellular acidification caused by secretion of protons from the cell. Other embodiments are related to an apparatus for determining a metabolic potential of a cell sample in a well of a multiwell plate.
Specimens in an embedding medium are attached to slides by heating (e.g., baking). Slides with the tissue sections or other embedded specimens are inserted in a slide basket which holds them vertically. The slide basket is placed into a heating chamber which has a sorbent support.
In alternative embodiments, provided are immunohistochemistry (IHC) methods for determining and scoring reproducibly the extent of nuclear expression of protein Ki-67 (also known as MK167) in a tissue sample. In alternative embodiments, provided are methods for diagnosing, treating or ameliorating or assessing the risk of recurrence for a cancer or a tumor using an IHC method as provided herein. In alternative embodiments, provided are kits comprising components and instructions for practicing methods as provided herein.
Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3′—OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.
A device for determining a target separation method for separating a fluidic sample by a target sample separation apparatus modifies an initial separation method for an initial sample separation apparatus. The device includes a data provision unit configured for providing a first initial data set characterizing the initial separation method, a second initial data set characterizing properties of the target sample separation apparatus, a third initial data set characterizing properties of the fluidic sample, a fourth initial data set characterizing properties of the initial sample separation apparatus, and a determining unit configured for determining a target data set characterizing the target separation method by carrying out a numerical analysis based on the first initial data set, the second initial data set, the third initial data set, and the fourth initial data set.
The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.
B01D 15/22 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
C23C 16/04 - Coating on selected surface areas, e.g. using masks
C23C 16/44 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating
C23C 16/455 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating characterised by the method used for introducing gases into the reaction chamber or for modifying gas flows in the reaction chamber
67.
SYSTEMS AND METHODS FOR TARGETED NUCLEIC ACID CAPTURE
The present disclosure provides systems and methods for targeted indirect, synergistic hybridization capture of a template for amplification and analysis of target sequences. The captured templates can be further treated with bisulfite or other methylation reagents to study the methylation pattern of the nucleic acid molecules of the template.
C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6818 - Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
In some examples, an electron capture dissociation (ECD) cell may include a plurality of lenses. At least one lens of the plurality of lenses may be asymmetrically arranged about an axis that is orthogonal to a central axis of the ECD cell and intersects a center-point along a length of the ECD cell defined along the central axis.
The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.
B01D 15/22 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
C23C 16/04 - Coating on selected surface areas, e.g. using masks
C23C 16/44 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating
C23C 16/455 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating characterised by the method used for introducing gases into the reaction chamber or for modifying gas flows in the reaction chamber
70.
METAL COMPONENTS WITH INERT VAPOR PHASE COATING ON INTERNAL SURFACES
The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.
B01D 15/22 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
C23C 16/04 - Coating on selected surface areas, e.g. using masks
C23C 16/44 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating
C23C 16/455 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating characterised by the method used for introducing gases into the reaction chamber or for modifying gas flows in the reaction chamber
71.
METAL COMPONENTS WITH INERT VAPOR PHASE COATING ON INTERNAL SURFACES
The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.
B01D 15/22 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
C23C 16/04 - Coating on selected surface areas, e.g. using masks
C23C 16/44 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating
C23C 16/455 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating characterised by the method used for introducing gases into the reaction chamber or for modifying gas flows in the reaction chamber
72.
METAL COMPONENTS WITH INERT VAPOR PHASE COATING ON INTERNAL SURFACES
The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.
B01D 15/22 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
C23C 16/04 - Coating on selected surface areas, e.g. using masks
C23C 16/44 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating
C23C 16/455 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating characterised by the method used for introducing gases into the reaction chamber or for modifying gas flows in the reaction chamber
73.
FLUID SUPPLY DEVICES FOR FORMING A FILTERED MOBILE PHASE FOR A SAMPLE SEPARATING DEVICE
A fluid supply device for providing a mobile phase for a sample separating device includes a supply path configured to provide a fluid for forming at least a part of the mobile phase, a fluid conveying unit configured to receive and pressurize the fluid from the supply path and to convey the fluid to the sample separating device, and a sterile filter disposed in the supply path upstream of the fluid conveying unit and configured to filter the fluid. The fluid supply device may include a pre-filter pump configured to push the fluid through the sterile filter and/or a buffer unit configured to buffer the fluid.
The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.
B01D 15/22 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
C23C 16/04 - Coating on selected surface areas, e.g. using masks
C23C 16/44 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating
C23C 16/455 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating characterised by the method used for introducing gases into the reaction chamber or for modifying gas flows in the reaction chamber
75.
METAL COMPONENTS WITH INERT VAPOR PHASE COATING ON INTERNAL SURFACES
The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.
B01D 15/22 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
C23C 16/04 - Coating on selected surface areas, e.g. using masks
C23C 16/44 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating
C23C 16/455 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating characterised by the method used for introducing gases into the reaction chamber or for modifying gas flows in the reaction chamber
76.
METAL COMPONENTS WITH INERT VAPOR PHASE COATING ON INTERNAL SURFACES
The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.
B01D 15/22 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
C23C 16/04 - Coating on selected surface areas, e.g. using masks
C23C 16/44 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating
C23C 16/455 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating characterised by the method used for introducing gases into the reaction chamber or for modifying gas flows in the reaction chamber
77.
DEPARAFFINIZATION OF TISSUE BY ELECTRIC FIELD GENERATION AND IONIZATION
Paraffin-embedded tissue, which may be disposed on a solid substrate, is prepared by a dry technique that removes paraffin from tissue without adding any liquid to the tissue, thereby rendering the tissue substantially free of paraffin. The dry technique may entail applying heat energy to the tissue effective to melt the paraffin and thereby render it flowable, and applying an electric field. The electric field is effective to impart electrical charge to the paraffin and to move the paraffin out from the tissue due to electrical charge repulsion or attraction, which may be assisted by moving an electrode utilized to generate the electric field relative to the paraffin. The electric field, or both the electric field and the heat energy, may be applied until the tissue is substantially free of paraffin.
A sample handling device for handling a sample container, in particular for an analysis device for analyzing a fluidic sample in the sample container, includes: i) a push-push mechanism for fixing and releasing the sample container; and ii) a coupling region for releasably coupling to a sample moving device. The sample handling device is a passive device which is adapted to perform the fixing and the releasing free of a drive, in particular a motor. Furthermore, a related sample handling arrangement, an analysis device, and a method are described.
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
79.
Visual Interface for Generation of Groups and Experiment Building
A user interface that includes a plate map that automatically updates to provide indicia descriptive of parameter assignments and parameter groups can provide an intuitive system for building experiments. For example, a user may provide one or more inputs to generate and assign a parameter setting to a plurality of cells that represent a plurality of wells. The user interface may update the plate map to depict one or more indicia to indicate which wells have been assigned the new parameter setting.
G16H 10/40 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
Disclosed is an analytical device (100), comprising: i) a fluid compartment (120), configured to accommodate a solvent; ii) a blocking device (125), configured to close an input (121) and/or an output (122) of the fluid compartment (120); iii) a flow sensor (110) and/or a pressure sensor, coupled to the fluid compartment (120), and configured to perform a measurement with respect to the solvent; iv) a temperature change device (130), coupled to the fluid channel (120), and configured to perform a temperature change with respect to the fluid compartment (120); and v) a determination device, configured to determine a thermal property of the solvent based on the measurement and the temperature change.
A substrate orientation device includes a frame that supports a substrate on which liquid is dispensed, for example as may relate to (bio)chemical microarray fabrication. The frame is movable to different positions, such as for mounting the substrate to the frame, depositing droplets on the substrate, and applying a bulk liquid to the substrate. The substrate orientation device may be movable to different locations, such as different liquid dispensing devices, for example a printer and a flow cell.
In alternative embodiments, provided are immunohistochemistry (IHC) methods for determining and scoring reproducibly the extent of expression of the protein Melanoma Associated Antigen Gene-A4 (MAGE-A4), in a tissue sample. In alternative embodiments, provided are methods for diagnosing, treating or ameliorating or assessing the risk of recurrence for a cancer or a tumor using an IHC method as provided herein. In alternative embodiments, provided are kits comprising components and instructions for practicing methods as provided herein. The present application describes methods for scoring MAGE-A4 expression and utilizing the score as a companion or complementary diagnostic or to treat or ameliorate cancer or a tumor.
Extended duration cell analysis is provided via a device, comprising: a chamber configured to accept: a sample carrier, that includes a plurality of wells having electrodes in electrical communication with an impedance meter; and a cartridge, including a compound and at least one delivery port for the compound; a camera configured to capture images of a cell culture in each well via associated windows in the wells when the sample carrier is positioned at a first location; a fluid handler, in communication with the sample carrier and the cartridge when the sample carrier is positioned at a second location, configured to deliver the compound from the cartridge to a given well based on an impedance measurement of a sample in the given well; and a motion stage configured to move the sample carrier between the first location and the second location.
Systems and methods are disclosed for capturing and interpreting data streams between an instrument and a controlling device. A processor is configured to receive a data stream sent by the instrument to the controlling device and identify data frames in the stream. The processor is configured to search for a bit pattern in the stream, identify bits corresponding to a message length of a first presumed data frame based on a location relative to the bit pattern, and identify a second presumed data frame in the data stream based on the message length of the first presumed data frame. The processor is further configured to identify a second instance of the bit pattern, increase a count, continue scanning, extract and store instrument measurement data or operational metadata from the identified data frames, and analyze or interpret the captured data and metadata for visualization or alerts.
H04Q 9/00 - Arrangements in telecontrol or telemetry systems for selectively calling a substation from a main station, in which substation desired apparatus is selected for applying a control signal thereto or for obtaining measured values therefrom
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor
85.
Substrate orientation device for liquid dispensing operations
A substrate orientation device includes a frame that supports a substrate on which liquid is dispensed, for example as may relate to (bio)chemical microarray fabrication. The frame is movable to different positions, such as for mounting the substrate to the frame, depositing droplets on the substrate, and applying a bulk liquid to the substrate. The substrate orientation device may be movable to different locations, such as different liquid dispensing devices, for example a printer and a flow cell.
Extended duration cell analysis is provided via a device, comprising: a chamber configured to accept: a sample carrier, that includes a plurality of wells having electrodes in electrical communication with an impedance meter; and a cartridge, including a compound and at least one delivery port for the compound; a camera configured to capture images of a cell culture in each well via associated windows in the wells when the sample carrier is positioned at a first location; a fluid handler, in communication with the sample carrier and the cartridge when the sample carrier is positioned at a second location, configured to deliver the compound from the cartridge to a given well based on an impedance measurement of a sample in the given well; and a motion stage configured to move the sample carrier between the first location and the second location.
A liquid dispensing device provides an input flow of liquid from a single inlet into an internal manifold, and distributes or divides the input flow from the manifold into a group of individual liquid circuits that direct respective output flows to outlets, from which controlled volumes of liquid can be dispensed into respective containers such as the wells of a multi-well plate. The volume of liquid dispensed into one container may be different from that dispensed into another container. Each liquid circuit may include a valve that regulates the liquid flow in that liquid circuit, which may be based on feedback from flow rate sensors. Two or more liquid dispensing devices may be stacked horizontally to provide additional unique liquid circuits and different liquids if desired.
41 - Education, entertainment, sporting and cultural services
42 - Scientific, technological and industrial services, research and design
Goods & Services
Data analysis; Data processing; Data collection, compilation, and systemization; Data management; Data research; Updating and maintenance of data; Business information, advice, and consultation; Business management services; Consumer loyalty services Providing online newsletters; On-line electronic newsletters delivered by email; Providing publications, videos, data, news and information, images, software, applications, links, online courses, tutorials, webinars, event information, special offers, and tools and other resources; Providing a website featuring publications, videos, data, news and information, images, software, applications, links, online courses, tutorials, webinars, event information, and tools and other resources Providing information about medical and scientific research; Providing scientific information, advice and consultancy; Scientific research, development, and consultancy services; Design and development services
An autoclaving microplate washing system for cells and non-adhering three-dimensional (3D) cell cultures includes one or more pumps for controlling the dispensing of washing fluid and the evacuation of fluid from microwells to gently wash the cells. A method of controlling the autoclaving microplate washing system includes controlling the one or more pumps for dispensing and evacuation.
B05B 1/14 - Nozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means with multiple outlet openingsNozzles, spray heads or other outlets, with or without auxiliary devices such as valves, heating means with strainers in or outside the outlet opening
B08B 3/02 - Cleaning by the force of jets or sprays
B08B 11/02 - Devices for holding articles during cleaning
F04B 13/00 - Pumps specially modified to deliver fixed or variable measured quantities
F04B 23/06 - Combinations of two or more pumps the pumps being all of reciprocating positive-displacement type
A liquid dispensing device provides an input flow of liquid from a single inlet into an internal manifold, and distributes or divides the input flow from the manifold into a group of individual liquid circuits that direct respective output flows to outlets, from which controlled volumes of liquid can be dispensed into respective containers such as the wells of a multi-well plate. The volume of liquid dispensed into one container may be different from that dispensed into another container. Each liquid circuit may include a valve that regulates the liquid flow in that liquid circuit, which may be based on feedback from flow rate sensors. Two or more liquid dispensing devices may be stacked horizontally to provide additional unique liquid circuits and different liquids if desired.
In some examples, linear and non-linear range-based plot pane selection may include receiving data that is to be displayed. Based on an increase or a decrease in a size of a range selector, a selection of a range of plot panes may be received from a plurality of available plot panes to display the data. Based on the received selection of the range of plot panes, a display of the data may be generated in plot panes included in the range of plot panes. The size of the range selector may be non-linearly proportional to the available plot panes to display the data.
G06F 3/0482 - Interaction with lists of selectable items, e.g. menus
G06F 3/04845 - Interaction techniques based on graphical user interfaces [GUI] for the control of specific functions or operations, e.g. selecting or manipulating an object, an image or a displayed text element, setting a parameter value or selecting a range for image manipulation, e.g. dragging, rotation, expansion or change of colour
95.
CHROMATOGRAPHY COLUMN FOR THE SEPARATION OF SURFACTANTS
A chromatographic separation column for characterizing surfactant purity is disclosed. The column includes a separation medium having a particulate porous substrate with monofunctional silane with diisopropyl side chain groups and a pendant cyano functional group held within a column. The porous substrate has a pore size of about 50 Å to about 500 Å and an average particle size of about 1.0 μm to about 100 μm. The column has an inner diameter of about 1.0 to 100 mm, e.g., 4.6 mm and a length from about 10 mm to about 250 mm. Methods of facilitating characterization of polysorbate 80 by providing a sample containing polysorbate 80 and one or more reaction products of polysorbate 80 and a chromatographic separation column are disclosed. Methods of characterizing polysorbate 80 by separating polysorbate 80 and one or more reaction products of polysorbate 80 are also disclosed.
G01N 30/88 - Integrated analysis systems specially adapted therefor, not covered by a single one of groups
B01D 15/10 - Selective adsorption, e.g. chromatography characterised by constructional or operational features
B01J 20/28 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof characterised by their form or physical properties
B01J 20/286 - Phases chemically bonded to a substrate, e.g. to silica or to polymers
B01J 20/30 - Processes for preparing, regenerating or reactivating
In some examples, a multi-device removal and installation tool may include a device removal tool including a first side having a first receiver with a first dimension that removably receives a first device, and a second side having a second receiver with a second dimension that removably receives a second device. The first dimension may be different from the second dimension. Further, the first receiver may include a plurality of separators to separate a surface of the first receiver from a surface of the first device.
B25B 27/14 - Hand tools or bench devices, specially adapted for fitting together or separating parts or objects whether or not involving some deformation, not otherwise provided for for assembling objects other than by press fit or detaching same
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
C07K 16/18 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans
C07K 16/00 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies
The present invention discloses a gas chromatography (GC) system for separating analytes from a matrix, comprising: a GC system entrance; a first column fluidically connected to the GC system entrance through a first valve; a second column fluidically connected to the first column through a second valve; a third column fluidically connected to the first column through the second valve; a fourth column fluidically connected to a third column through a third valve; and a GC system exit fluidically connected to both the fourth column and the second column through a fourth valve. And the present invention also discloses a method of separating various analytes, including analyte portions comprising one or more of Ar, O2, N2, CH4 and CO2, from a gas matrix and from CO2 by using the GC system.
G01N 30/46 - Flow patterns using more than one column
B01D 53/02 - Separation of gases or vapoursRecovering vapours of volatile solvents from gasesChemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases or aerosols by adsorption, e.g. preparative gas chromatography
99.
FLUIDICALLY COUPLING OF SAMPLING AND SEPARATION PATHS
A switching unit is configured for selectively fluidically coupling a sampling volume, a sampling drive, a mobile phase drive, and a separating device. In a sample load configuration, the switching unit is configured for fluidically coupling the sampling volume and the sampling drive, for moving the fluidic sample into the sampling volume. In a decouple configuration, the switching unit is configured for fluidically coupling the sampling volume between the sampling drive and the separating device, while the mobile phase drive is fluidically decoupled from the separating device. In a sample introduction configuration, the switching unit is configured for fluidically coupling the mobile phase drive, the sampling volume, and the separating device for introducing at least an amount of the fluidic sample stored in the sampling volume into the mobile phase for fluid separation by the separating device.
A process of operating an analytical device (100) for analyzing a sample, the process comprising monitoring a present user behavior when operating the analytical device (100), comparing the monitored present user behavior with a prior user behavior, and when determining a discrepancy between the monitored present user behavior and the prior user behavior, triggering an action.
