An analysis device for carrying out an analysis method includes multiple defined positions at which process fluids can be received. A defined position is selected. The selected position is mechanically provided to a user and/or a sample handling device. The selection is based on the content of the process fluids and/or a user behavior with respect to the process fluids. The analysis device may be configured for performing sample separation, such as by chromatography or electrophoresis.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
G01N 30/88 - Systèmes intégrés d'analyse, spécialement adaptés à cet effet, non couverts par un seul des groupes
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, eliminate the use of, or reduce the dependence on formamide in hybridization. Compositions for use in the invention include an aqueous composition comprising at least one nucleic acid sequence and at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
Produits et services
Reagents for scientific and research use; Diagnostic reagents and preparations, except for medical or veterinary use; Nucleic acid sequences and chemical reagents, other than for medical or veterinary purposes Biological reagents for medical purposes; Medical diagnostic reagents; Nucleic acid sequences and chemical reagents for medical purposes; Reagents for medical purposes
In order to provide an analysis method and device using laser ablation and a plasma ion source, without requiring a solid standard, embodiments include a plasma element analysis method and device, wherein a solution standard is atomized by a nebulizer and introduced into a plasma, calibration curve data for an element contained in the solution standard is created, a solid sample to be measured is atomized by laser ablation, the solid sample is introduced into the plasma together with an auxiliary liquid from the nebulizer and ionized, element analysis data is created, a concentration of a measured element in the solid sample is acquired from the element analysis data and the calibration curve data, and the concentration of the measured element is corrected and quantified.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/00 - Spectromètres pour particules ou tubes séparateurs de particules
In some examples, an ion guide may include at least four rods spanning a portion of a circumference of the ion guide, and extending along a central axis of the ion guide. The ion guide may further include a plurality of electrodes. Each electrode of the plurality of electrodes may span a remaining portion of the circumference of the ion guide. Further, the plurality of electrodes may extend, in a side-by-side arrangement of electrodes of the plurality of electrodes, along the central axis of the ion guide.
Methods and systems for carrier gas identification in gas chromatography are described herein. In one aspect, a gas chromatography system can include a pneumatic system including an input flowpath in fluidic communication with a first output flowpath and a second output flowpath; a first flow sensor configured to generate a flow measurement signal corresponding to a first property of a gas; a second flow sensor configured to generate a second flow measurement signal corresponding to a second property of the gas different than the first gas property; and a controller programmed to: determine the first flow measurement signal for the flow of gas through the pneumatic system; determine the second flow measurement signal for the flow of gas through the pneumatic system; and identify a type of gas for the flow of gas through the pneumatic system from the first and second flow measurement signals.
B01D 53/02 - Séparation de gaz ou de vapeursRécupération de vapeurs de solvants volatils dans les gazÉpuration chimique ou biologique des gaz résiduaires, p. ex. gaz d'échappement des moteurs à combustion, fumées, vapeurs, gaz de combustion ou aérosols par adsorption, p. ex. chromatographie préparatoire en phase gazeuse
The present disclosure relates to a method of assembling long nucleic acids by enzymatically ligating oligonucleotide molecules hybridized to an indexed splint oligonucleotide molecules. Also disclosed are oligonucleotide structures comprising an indexed splint oligonucleotide useful in performing the disclosed method.
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
9.
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY (HPLCZMS)-BASED APPARATUS AND METHOD FOR MONITORING OF LIPID STABILITY IN MESSENGER RIBONUCLEIC ACID (MRNA) ENCAPSULATED LIPID NANOPARTICLES (LNPS) UNDER DIFFERENT STORAGE CONDITIONS
According to examples disclosed herein, a method for monitoring of lipid stability in a messenger ribonucleic acid (mRNA) encapsulated lipid nanoparticles (LNPs) composition in a test sample that has been exposed to a different condition than a control sample, where the mRNA encapsulated LNPs composition comprises a plurality of LNP lipids, may include performing high-performance liquid chromatography/mass spectrometry (HPLC/MS) analysis on the control sample and the test sample to measure an amount of each LNP lipid of the mRNA encapsulated LNPs composition. The method may further include comparing the amount of each LNP lipid in the control sample to the amount of each LNP lipid in the test sample. A change in an amount of one of the plurality of LNP lipids determined based on the comparison may indicate an instability of the mRNA encapsulated LNPs composition in the test sample.
G01N 23/2258 - Recherche ou analyse des matériaux par l'utilisation de rayonnement [ondes ou particules], p. ex. rayons X ou neutrons, non couvertes par les groupes , ou en mesurant l'émission secondaire de matériaux en utilisant des microsondes électroniques ou ioniques en utilisant des faisceaux d’ions incidents, p. ex. des faisceaux de protons en mesurant l’émission d’ions secondaires, p. ex. spectrométrie de masse à ionisation secondaire [SIMS]
G01N 27/626 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant l'ionisation des gaz, p. ex. des aérosolsRecherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant les décharges électriques, p. ex. l'émission cathodique utilisant la chaleur pour ioniser un gaz
G01N 30/88 - Systèmes intégrés d'analyse, spécialement adaptés à cet effet, non couverts par un seul des groupes
Systems and methods for heater assemblies are described herein. A gas chromatography device can include a heater assembly. The heater assembly can include a plate. The plate can have an inner diameter, an outer diameter, a planar surface, and a curved surface. The heater assembly can include a wire. The wire can form a plurality of coils around the inner diameter and the outer diameter of the plate. Each of the plurality of coils can have a diameter greater than half of a difference between the outer diameter of the plate and the inner diameter of the plate. Each of the plurality of coils can be configured to intersect the inner diameter of the plate and the outer diameter of the plate. An aspect ratio of half of the difference between the outer diameter of the plate and the inner diameter of the plate to a thickness of the plate is greater than 1. The device can include a mounting piece configured to couple with the heater assembly.
Systems and methods for heater assemblies are described herein. A gas chromatography device can include a heater assembly. The heater assembly can include a plate. The plate can have an inner diameter, an outer diameter, a planar surface, and a curved surface. The heater assembly can include a wire. The wire can form a plurality of coils around the inner diameter and the outer diameter of the plate. Each of the plurality of coils can have a diameter greater than half of a difference between the outer diameter of the plate and the inner diameter of the plate. Each of the plurality of coils can be configured to intersect the inner diameter of the plate and the outer diameter of the plate. A radial width can be defined by half of the difference between the outer diameter of the plate and the inner diameter of the plate. The radial width can be greater than a thickness of the plate.
H05B 3/16 - Éléments chauffants caractérisés par la composition ou la nature des matériaux ou par la disposition du conducteur le conducteur étant monté sur une base isolante
In some examples, an ion trap may include an ion trap controller to apply a trapping potential and scanning voltage waveforms to at least one electrode to sequentially release higher mass-to-charge (m/z) ions before lower m/z ions. The ion trap may further include a linear configuration. Ion ejection from the ion trap may be perpendicular to an axis of the ion trap.
A sampling device for an analytical device includes an object handling device, in particular a robotic arm, configured for handling an object such as an analytical sample. A needle is fixedly coupled to the object handling device. A driving device, coupled to the object handling device, is configured for providing a driving force to the object handling device to drive the object handling device, such as in the vertical direction. A movable element is movably coupled to the object handling device, such that the movable element is at least partially movable with respect to the fixedly coupled needle. A holding element is configured for providing a holding force to the movable element, such that the holding force holds the movable element against the driving force. A control device is configured to increase the driving force until the driving force overcomes the holding force at a break-off region.
G01N 1/14 - Dispositifs d'aspiration, p. ex. pompesDispositifs d'éjection
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
14.
CHARACTERIZING A PROPERTY OF A FLOW PATH OF AN ANALYSIS DEVICE
In a method for characterizing a property of a flow path, in particular of an element in the flow path, in an analysis device, the volume of a fluid in the flow path, in particular in the element of the flow path, is changed in order to change the pressure in the flow path. The pressure change in the flow path as a consequence of the volume change is determined. The property of the flow path is characterized based on the determined pressure change and/or the volume change.
The present disclosure provides new reagents for labeling and detecting O-glycans released from a glycoconjugate, such as a glycoprotein, glycopeptide, peptidoglycan, or proteoglycan of interest. O-glycans labeled with the labels can be detected by fluorescence, MS, and UV. The invention further provides methods for making the reagents, methods for using them, and kits comprising them. O-glycans labeled with the reagents can be analyzed by high-throughput analysis.
G01N 30/88 - Systèmes intégrés d'analyse, spécialement adaptés à cet effet, non couverts par un seul des groupes
G01N 33/533 - Production de composés immunochimiques marqués avec un marqueur fluorescent
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
G01N 33/74 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des hormones
A microtiter plate assembly is disclosed that includes a base having a plurality of wells and a lid having a plurality of projections corresponding to the plurality of wells. Each projection contains a radial notch and a canted distal tip, providing bubble free sealing, reduced oxygen back-diffusion, and increased sensitivity even at small sample volumes.
A positioning device includes a first positioning member and a second positioning member, at least one of which is a movable member. The first positioning member includes ramps and first, second, and third interface elements. The movable member is linearly translatable in parallel with the other member such that fourth, fifth and sixth interface elements of the second positioning member slide along the ramps and into contact with the first, second and third interface elements, respectively, at a coupled position. At the coupled position, the members form a kinematic system that constrains six degrees of freedom of movement of the movable member to thereby position, in a precise and repeatable manner, an object mounted to the movable member. The positioning device may be utilized, for example, in a quick connect mechanism for an optics-based apparatus.
G01B 11/26 - Dispositions pour la mesure caractérisées par l'utilisation de techniques optiques pour mesurer des angles ou des cônesDispositions pour la mesure caractérisées par l'utilisation de techniques optiques pour tester l'alignement des axes
19.
CONTROLLING ANTIBODY SPECIFICITIES IN A POLYCLONAL HUMORAL RESPONSE BY EPITOPE TUNING
In alternative embodiments, provided are chimeric immunogens, and methods for making and using them, including methods for making and obtaining polyclonal antibodies specific for selected epitopes. In alternative embodiments, provided are methods for generating a balanced immune response against a plurality of epitopes present in an antigen comprising immunizing a host with a plurality of polypeptides, wherein each polypeptide comprises one or a subset of said plurality of epitopes. In alternative embodiments, provided are methods for generating a balanced, epitope-specific antibody response in a non-human mammalian host, wherein the immune response comprises generation of host antibodies specifically against (or that specifically bind to) at least one human epitope, and the method comprises administering to the host a sufficient amount of a chimeric or recombinant polypeptide to generate the epitope-specific antibody response.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
Integrated cell analysis on biological cells sampled from a bioreactor biological cells from a bioreactor for integrated cell analysis may be provided by: a bioreactor; a sensor; a processor; a memory including instructions that when executed by the memory perform operations that comprise, automatically: extracting a sample from cells being cultured in the bioreactor; preparing the sample to produce a prepared sample; analyzing the prepared sample to identify a value for an associated cell analysis parameter; and in response to determining that the value is outside a window for the associated cell analysis parameter, adjusting a setting of the bioreactor based on a difference between the value and the window.
09 - Appareils et instruments scientifiques et électriques
Produits et services
(1) Mass detection systems; instruments for mass spectrometry measurements; liquid chromatography mass spectrometry systems; liquid chromatography mass detection systems; scientific instruments, namely, liquid chromatography mass spectrometers, including liquid delivery system (pump), injection system, autosampler, column compartment, valving, ion source generation, and calibrant delivery systems; software for instrument control, instrument tuning, signal collection, data analysis, and reporting of the output of liquid chromatography mass spectrometers; liquid chromatography mass spectrometry detection systems for biological and chemical separations, measurements, screening, targeted analysis, and identification.
09 - Appareils et instruments scientifiques et électriques
Produits et services
(1) Mass detection systems; instruments for mass spectrometry measurements; liquid chromatography mass spectrometry systems; liquid chromatography mass detection systems; scientific instruments, namely, liquid chromatography mass spectrometers, including liquid delivery system (pump), injection system, autosampler, column compartment, valving, ion source generation, and calibrant delivery systems; software for instrument control, instrument tuning, signal collection, data analysis, and reporting of the output of liquid chromatography mass spectrometers; liquid chromatography mass spectrometry detection systems for biological and chemical separations, measurements, screening, targeted analysis, and identification.
In a pump head for a scroll pump, an orbiting scroll is coupled to a crank of a crankshaft. As the crankshaft rotates, the crank drives the orbiting scroll to orbit relative to one or more fixed scrolls to pump fluid from an inlet to an outlet. A stub shaft is attached to the crank and may be removed as part of an at least partial disassembly of the pump head. An adjusting nut may adjust an axial position of the orbiting scroll relative to the fixed scroll(s). The adjusting nut may be removed, and thereafter reinstalled, together with the stub shaft without altering an axial position of the adjusting nut relative to the stub shaft.
F04C 29/00 - Parties constitutives, détails ou accessoires de pompes ou d'installations de pompage spécialement adaptées pour les fluides compressibles non couverts dans les groupes
F04C 18/02 - Pompes à piston rotatif spécialement adaptées pour les fluides compressibles du type à engrènement, c.-à-d. avec un mouvement de translation circulaire des organes coopérants, chaque organe possédant le même nombre de dents ou de parties équivalentes de prise
A sample handling device for handling a fluid sample in an analyzer for analyzing the fluid sample includes a sample needle having a lumen (197) through which the fluid sample can be passed, and a movement apparatus for moving the sample needle. The sample needle is rotatably mounted with respect to the movement apparatus.
The present disclosure provides methods and apparatuses for generating training data, training a machine model, and analyzing a biologic sample. The generating of the training data includes obtaining a first image of a biological sample; generating a first set of annotations of the first image based on a second image of said biological sample stained by a quantitative approach converting antibody/antigen complexes into dots; and outputting the first image and a ground truth including said first set of annotations as data for training a first machine learning model ("ML") for analyzing a biological sample. Moreover, the generated (output) training data may then be used for training an ML model and the train ML model may be used for analyzing the biologic sample.
G06V 10/774 - Génération d'ensembles de motifs de formationTraitement des caractéristiques d’images ou de vidéos dans les espaces de caractéristiquesDispositions pour la reconnaissance ou la compréhension d’images ou de vidéos utilisant la reconnaissance de formes ou l’apprentissage automatique utilisant l’intégration et la réduction de données, p. ex. analyse en composantes principales [PCA] ou analyse en composantes indépendantes [ ICA] ou cartes auto-organisatrices [SOM]Séparation aveugle de source méthodes de Bootstrap, p. ex. "bagging” ou “boosting”
G06V 10/82 - Dispositions pour la reconnaissance ou la compréhension d’images ou de vidéos utilisant la reconnaissance de formes ou l’apprentissage automatique utilisant les réseaux neuronaux
G06V 20/69 - Objets microscopiques, p. ex. cellules biologiques ou pièces cellulaires
27.
AUTOMATED DETECTION OF DOTS IN IMAGES OF STAINED BIOLOGICAL SAMPLES
The present disclosure provides methods and apparatuses for detecting objects in an image of a biological sample. In particular, an image is obtained of a biological sample stained with a quantitative approach converting antibody/antigen complexes into dots. The dots are detected in said image using a trained artificial intelligence ("AI") model that has been pre-trained to detect dots. Moreover, methods and apparatuses for training such AI model are provided.
G06V 10/82 - Dispositions pour la reconnaissance ou la compréhension d’images ou de vidéos utilisant la reconnaissance de formes ou l’apprentissage automatique utilisant les réseaux neuronaux
G06V 20/69 - Objets microscopiques, p. ex. cellules biologiques ou pièces cellulaires
28.
SYSTEMS AND METHODS FOR TARGETED NUCLEIC ACID CAPTURE AND BARCODING
The present disclosure provides systems and methods for adding barcode sequences by primer extension to target nucleic acid molecules, as part of the targeted indirect, synergistic hybridization capture of the target for amplification and analysis of target sequences. Barcode sequences such sample index sequences and/or unique molecular identifier sequences are provided on an extension template which hybridizes with an adaptor attached to the target molecule.
Systems and methods for efficiently detecting whether an air bubble is present in an immersion liquid between a liquid immersion objective and a body are provided. For example, an automated imaging system including a liquid immersion objective, a light source configured to emit a first light to a body via the liquid immersion objective in a state in which an immersion liquid is on the liquid immersion objective between the liquid immersion objective and the body, wherein the body is configured to hold a sample, a first sensor configured to receive a first signal based on the first light, after the first light arrives at the body via the liquid immersion objective, and a controller configured to detect whether an air bubble is present in the immersion liquid between the liquid immersion objective and the body based on the first signal received by the first sensor.
Data-driven bioreactor cultivation of immune cells may be provided by dividing a first sample of immune cells into first and second populations; cultivating the first and second populations in respective first and second bioreactor to produce respective first and second cultivated populations; measuring a respective values for a potency parameter, a metabolic parameter, or both, at a harvest time for each cultivated population of immune cells; and after the harvest time: collecting a second sample of the immune cells; selecting one of the first bioreactor or the second bioreactor based on which of the respective cultivated populations was measured with a higher value for the potency parameter, a higher value for the metabolic parameter, or both; and cultivating the second sample of the immune cells in the one of the first bioreactor and the second bioreactor selected to produce a cultivated second sample.
An injector, for injecting a fluidic sample into a flow path between a fluid drive and a sample separation unit, includes a sample accommodation volume, a sample drive, and a fluidic valve switchable to selectively couple the volume with the flow path or decouple the volume from the flow path. In an injection switching state, the fluid drive, the separation unit and the sample drive are coupled by the valve so that fluid driven by the sample drive and flowing from the volume to the separation unit and further fluid driven by the fluid drive and flowing from the fluid drive to the separation unit are combined at a fluidic connection upstream of the separation unit. In that same injection switching state, the sample drive can branch off at least a portion of an amount of mobile phase into the sampling path from such fluidic connection to flush at least a portion of the sampling path with mobile phase. A control unit controls the operation of the injector in the switching states.
Provided herein are workflows to isolate both metabolites and lipids ("2in1"), or metabolites, lipids and proteins ("3in1"). The 2in1 methods comprise treating a sample containing metabolites and lipids with a mixture comprising methanol and ethanol, which precipitates proteins, adding water to increase metabolite recoveries, and passing the treated sample through a solid phase extraction (SPE) matrix which has an affinity for lipids. The flow-through from the SPE matrix contains metabolites, and lipids can be eluted from the SPE matrix with a lipid elution solution. The 3in1 methods are similar to the 2in1, but proteins are pelleted prior to SPE extraction, and it is the pellet that is then analyzed for proteins.
C11B 1/10 - Production des graisses ou huiles à partir de matières premières par extraction
G01N 19/00 - Recherche sur les matériaux par des procédés mécaniques
G01N 30/00 - Recherche ou analyse de matériaux par séparation en constituants utilisant l'adsorption, l'absorption ou des phénomènes similaires ou utilisant l'échange d'ions, p. ex. la chromatographie
In some examples, a method comprises specifying a sorbent formulation for a targeted class of food contaminant analysis, and utilizing enhanced matrix removal (EMR) mixed-mode passthrough cleanup with the specified sorbent formulation. In other examples, an EMR cartridge may include a specified sorbent formulation for a targeted class of food contaminant analysis. The EMR cartridge may utilize EMR mixed-mode passthrough cleanup with the specified sorbent formulation.
G01N 30/50 - Conditionnement de l'absorbant ou de l'adsorbant ou de la phase liquide stationnaire
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
B01J 20/20 - Compositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation contenant une substance inorganique contenant du carbone libreCompositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation contenant une substance inorganique contenant du carbone obtenu par des procédés de carbonisation
B01J 20/22 - Compositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation contenant une substance organique
In some examples, an apparatus may include analyzing a sample, and analyzing at least one reference sample. A determination may be made as to whether the sample includes a library match score. Based on a determination that the sample includes the library match score, the library match score and a retention time may be analyzed to determine similarity between the sample and the at least one reference sample.
Purification methods are provided for oligonucleotides yielding purified 5'-phosphate terminated oligonucleotide. Also disclosed are methods of purification of oligonucleotides that are suited for synthetic RNA, particularly RNA made using the "TC-RNA" chemistry. The method for preparing a purified 5'-phosphate oligonucleotide comprises: obtaining a support-bound oligonucleotide, wherein the oligonucleotide has a free 5'-hydroxyl group and is attached through a linker via its 3'-hydroxyl or 3'-phosphate to a solid support; reacting the free 5'-hydroxyl group of the support-bound oligonucleotide with a phosphoramidite comprising a substituent containing a cleavable linker and a protected hydroxyl group, wherein reacting forms an extended 5'-phosphoester support-bound oligonucleotide; deprotecting the protected hydroxyl group of the 5'-phosphoester support-bound oligonucleotide and then reacting it with a reagent comprising an affinity tag to form an affinity-tagged 5'-phosphoester support-bound oligonucleotide, wherein the affinity tag is linked to the extended 5'-phosphoester oligonucleotide through said cleavable linker.
C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques
C07H 21/02 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le ribosyle comme radical saccharide
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
37.
SAMPLE SEPARATION DEVICE WITH FLUID DRIVE UNIT DECOUPLING
A sample separation apparatus includes a fluid drive arrangement having a first fluid drive unit and a second fluid drive unit for driving a mobile phase along a flow path to a sample separation unit, a sample accommodation volume configured to accommodate the fluidic sample and to be selectively fluidically coupleable with the flow path or fluidically decoupleable from the flow path, and a control unit configured to control respective operations of the fluid drive units, and to control a fluidically decoupling of the second fluid drive unit from the flow path in a decoupled operation mode of the sample separation apparatus. The control unit is further configured to control, in the decoupled operation mode, the decoupled second fluid drive unit for fluidically coupling to the sample accommodation volume, and intaking sample into the sample accommodation volume.
In some examples, a system may include a housing attachable to a column and including a turn fitting and a receiver. The turn fitting may be movable relative to the receiver and along an axis of the housing between a neutral state and an attached state. In the neutral state, the turn fitting may be disposed at a first axial location along the axis of the housing. In the attached state, the turn fitting may be disposed at a second axial location along the axis of the housing.
There is described a device (100), preferably a sampling device (100) for an analytic device (10), the device (100) comprising: i) an object handling device (190), in particular a robotic arm, configured for handling an object to be handled, in particular a sample such as an analytical sample; ii) a calibration feature (120) configured for at least partially accommodating a protruding part (110, 115) of the object handling device (190); and iii) a control device (70), configured to: move the protruding part (110, 115) of the object handling device (190) into the calibration feature (120) in an approaching direction (Z), so that the calibration feature (120) guides the protruding part (110, 115), in a plane (XY) perpendicular to the approaching direction (Z), to a calibration position during movement in the approaching direction (Z), so that at least one property of the object handling device (190) in the plane (XY) perpendicular to the approaching direction (Z) is defined by or relative to the calibration position.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
41.
SAMPLE TRANSPORT DEVICE WITH PUMPING ELEMENT FOR AN ANALYZER
A sample transport device for transporting a fluidic sample, in particular for an analyzer for analyzing the fluidic sample, includes a sample receptacle for receiving and/or dispensing the fluidic sample; a moving device, in particular a robot arm, for moving the sample receptacle, and a pumping element arranged to be moved by the moving device to perform and/or trigger a pumping operation.
Methods of quantifying IgG antibodies containing high mannose N-glycans. Methods include immobilizing a protein capable of binding to IgG antibodies containing high mannose N-glycans to a substrate. Methods include adding a sample comprising IgG antibodies containing high mannose N-glycans to the immobilized protein to form a binding mixture. Methods further include adding a high mannose binder protein (HMB)-dye conjugate to the binding mixture such that the HMB-dye conjugate binds to high mannose N-glycans present in the sample. Methods include detecting the IgG antibodies containing high mannose N-glycans bound to the HMB-dye conjugate. Kits for the quantifying IgG antibodies containing high mannose N-glycans are also disclosed.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
In some examples, an apparatus may include a retention time (RT) receiver that is executed by at least one hardware processor, to receive glucose ladder retention times (RTs), and receive neutral and charged glycan RTs. An RT analyzer that is executed by the at least one hardware processor may determine, based on application of the glucose ladder RTs, and the neutral and charged glycan RTs to expected glucose unit (GU) values, adjusted GU values. Further, an expected RT generator that is executed by the at least one hardware processor may determine, based on the adjusted GU values, expected RTs for specified glycans.
In some examples, an apparatus may include a retention time (RT) receiver that is executed by at least one hardware processor, to receive glucose ladder retention times (RTs), and receive neutral and charged glycan RTs. An RT analyzer that is executed by the at least one hardware processor may determine, based on application of the glucose ladder RTs, and the neutral and charged glycan RTs to expected glucose unit (GU) values, adjusted GU values. Further, an expected RT generator that is executed by the at least one hardware processor may determine, based on the adjusted GU values, expected RTs for specified glycans.
The disclosure provides binding agents (e.g., antibodies) against isoform 1 of human ADAM9, as well as kits and methods for using the same (e.g., immunoassays) as part of a companion diagnostic and for other applications. In some aspects, the binding agents described herein may be used in assays for detecting non-small cell lung cancer (NSCLC), pancreatic cancer, triple-negative breast cancer (TNBC), gastroesophageal cancer (GC), prostate cancer, and renal cancer.
C07K 16/38 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre des inhibiteurs de protéase de structure peptidique
C12Q 1/37 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase faisant intervenir une peptidase ou une protéinase
G01N 33/573 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour enzymes ou isoenzymes
47.
SYSTEMS AND METHODS FOR A VENTLESS GAS CHROMATOGRAPHY MASS SPECTROMETRY INTERFACE
Systems and methods for a ventless GC-MS interface are described herein. The system can include a gas chromatograph. The system can include a mass spectrometer connected to the gas chromatograph by a mass spectrometer flow path. The system can include a fitting. The fitting can include a sealing surface and one or more gas purge flow paths. The fitting can be fluidically connected to the mass spectrometer. The system can include a column disposed in a ferrule.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.
C12N 15/11 - Fragments d'ADN ou d'ARNLeurs formes modifiées
C07H 21/02 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le ribosyle comme radical saccharide
Systems and methods for a ventless GC-MS interface are described herein. The system can include a gas chromatograph. The system can include a mass spectrometer connected to the gas chromatograph by a mass spectrometer flow path. The system can include a fitting. The fitting can include a sealing surface and one or more gas purge flow paths. The fitting can be fluidically connected to the mass spectrometer. The system can include a column disposed in a ferrule.
G01N 30/32 - Contrôle des paramètres physiques du fluide vecteur de la pression ou de la vitesse
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
50.
CAPILLARY CONDITIONING FOR PROTEIN ELECTROPHORESIS
A capillary conditioning solution is formulated for coating an inside surface of a capillary utilized for capillary electrophoresis (CE). The solution may be utilized to condition the capillary before a CE run, and may be applied to the capillary between one or more different CE runs to maintain the effectiveness of the conditioning.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
In some examples, an apparatus may include an ion source including at least one lens that is partitioned by at least one partition into at least two lens partitions. The at least two lens partitions may be connectable to a direct current (DC) bias power supply to bias the at least two lens partitions and a radio frequency alternating current (RF AC) voltage power supply to produce a dipolar RF field within the at least one lens.
In some examples, an apparatus may include an ion source including at least one lens that is partitioned by at least one partition into at least two lens partitions. The at least two lens partitions may be connectable to a direct current (DC) bias power supply to bias the at least two lens partitions and a radio frequency alternating current (RF AC) voltage power supply to produce a dipolar RF field within the at least one lens.
Apparatus and methods for automated transfer of slides into and/or out of a slide basket. One of the slides is raised above other slides in the slide basket, and a suction member on a slide gripper contacts the raised slide. A vacuum is drawn in the suction member sufficient for the suction member to grip the raised slide, and the slide gripper moves to withdraw the raised slide from the slot of the slide basket. The slide gripper moves the gripped slide to a slide processing module or other desired location.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
B01L 9/00 - Dispositifs de supportDispositifs de serrage
09 - Appareils et instruments scientifiques et électriques
Produits et services
Mass detection systems; instruments for mass spectrometry
measurements; liquid chromatography mass spectrometry
systems; liquid chromatography mass detection systems;
scientific instruments, namely, liquid chromatography mass
spectrometers, including liquid delivery system (pump),
injection system, autosampler, column compartment, valving,
ion source generation, and calibrant delivery systems;
software for instrument control, instrument tuning, signal
collection, data analysis, and reporting of the output of
liquid chromatography mass spectrometers; liquid
chromatography mass spectrometry detection systems for
biological and chemical separations, measurements,
screening, targeted analysis, and identification.
09 - Appareils et instruments scientifiques et électriques
Produits et services
Mass detection systems; instruments for mass spectrometry
measurements; liquid chromatography mass spectrometry
systems; liquid chromatography mass detection systems;
scientific instruments, namely, liquid chromatography mass
spectrometers, including liquid delivery system (pump),
injection system, autosampler, column compartment, valving,
ion source generation, and calibrant delivery systems;
software for instrument control, instrument tuning, signal
collection, data analysis, and reporting of the output of
liquid chromatography mass spectrometers; liquid
chromatography mass spectrometry detection systems for
biological and chemical separations, measurements,
screening, targeted analysis, and identification.
58.
DECONVOLUTION BY VISUAL ACUITY-BASED INTERPRETATION OF MASS SPECTROMETRY (MS) AND TANDEM MASS SPECTROMETRY (MS/MS) SPECTRA
In some examples, deconvolution by visual acuity-based interpretation of mass spectrometry (MS) and tandem mass spectrometry (MS/MS) spectra may include receiving, for an ion that is be identified, a plurality of expected ion spectra, where each expected ion spectrum of the plurality of expected ion spectra may include at least one expected peak profile, receiving an observed ion spectrum including at least one observed peak profile, and identifying characteristics of the expected ion spectra and the observed ion spectrum. The characteristics may be analyzed to determine ion scores, where a highest ion score may be used to identify a corresponding expected ion spectrum. An indication and/or a graphical user interface display of the expected ion spectrum corresponding to the highest ion score may be generated.
A configurable injector for injecting a fluidic sample in a separation path of a sample separation apparatus includes a sample accommodation volume for accommodating the fluidic sample to be injected into the separation path, a valve arrangement fluidically couplable with the separation path, fluidically coupled with the sample accommodation volume, and being controllable for injecting the fluidic sample into the separation path, an input interface configured for receiving input data indicative of an injection profile of an injector to be emulated by the configurable injector, and a control unit configured for controlling the configurable injector, in particular the valve arrangement, so that the configurable injector is operated in accordance with the injection profile to thereby emulate the injector to be emulated.
F16K 11/074 - Soupapes ou clapets à voies multiples, p. ex. clapets mélangeursRaccords de tuyauteries comportant de tels clapets ou soupapesAménagement d'obturateurs et de voies d'écoulement spécialement conçu pour mélanger les fluides dont toutes les faces d'obturation se déplacent comme un tout comportant uniquement des tiroirs à éléments de fermeture articulés à pivot à faces d'obturation planes
Methods and systems for estimating gas supply pressure are described herein. The method can include measuring a runtime valve duty cycle for a valve of a gas chromatography system. The method can include estimating a runtime supply pressure from the runtime valve duty cycle and at least one of a runtime flow rate or downstream pressure based on one or more calibration valve duty cycles corresponding to one or more calibration supply pressures and one or more calibration flow rates. The method can include, responsive to determining the runtime supply pressure is greater than a threshold supply pressure value, generating a notification that the runtime supply pressure is greater than the threshold supply pressure value.
G01N 30/32 - Contrôle des paramètres physiques du fluide vecteur de la pression ou de la vitesse
B01D 15/16 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives au conditionnement du fluide vecteur
Methods and systems for estimating gas supply pressure are described herein. The method can include measuring a runtime valve duty cycle for a valve of a gas chromatography system. The method can include estimating a runtime supply pressure from the runtime valve duty cycle and at least one of a runtime flow rate or downstream pressure based on one or more calibration valve duty cycles corresponding to one or more calibration supply pressures and one or more calibration flow rates. The method can include, responsive to determining the runtime supply pressure is greater than a threshold supply pressure value, generating a notification that the runtime supply pressure is greater than the threshold supply pressure value.
A column including particles having an average particle size ranging from about 1 μm to about 5 μm; the particles have an average pore size ranging from about 450 Å to about 3000 Å; and the particles have an average pore volume ranging from about 0.1 cm3/g to about 5 cm3/g is disclosed. The column can be a size exclusion chromatography column. The column can be used in a method to separate monomers from viral analytes, which is also disclosed.
A column including particles having an average particle size ranging from about 1 μm to about 5 μm; the particles have an average pore size ranging from about 450 Å to about 3000 Å; and the particles have an average pore volume ranging from about 0.1 cm3/g to about 5 cm3/g is disclosed. The column can be a size exclusion chromatography column. The column can be used in a method to separate monomers from viral analytes, which is also disclosed.
B01D 15/20 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives au conditionnement de la matière adsorbante ou absorbante
B01D 15/34 - Séparation par sélection en fonction de la taille, p. ex. chromatographie d'exclusion de tailleFiltration sur gelPerméation
B01J 20/28 - Compositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation caractérisées par leur forme ou leurs propriétés physiques
B01J 20/283 - Absorbants ou adsorbants poreux à base de silice
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chromatography apparatus for laboratory use; Measuring apparatus; Measuring instruments; Testing apparatus not for medical purposes; Material testing instruments and machines; Liquid chromatography equipment comprising pumps, injectors, autosamplers, column-compartments and columns, detectors, valves and valve-actuators; Laboratory operating systems comprising computer hardware and computer software for use in connection with liquid chromatography systems; Liquid chromatographs for laboratory use; Detectors.
A composition, including an alkaloid, and a diaryl ketone is disclosed. Also, disclosed is a kit including one or more containers in which each of the one or more containers includes the composition. A method of using a standard composition is also disclosed.
A composition, including an alkaloid, and a diaryl ketone is disclosed. Also, disclosed is a kit including one or more containers in which each of the one or more containers includes the composition. A method of using a standard composition is also disclosed.
A system for electronically and optically monitoring biological samples, the system including: a multi-well plate having a plurality of wells configured to receive a plurality of biological samples, each of the wells having a set of electrodes and a transparent window on a bottom surface of the well that is free of electrodes; an illumination module configured to illuminate the wells; a cradle configured to receive the multi-well plate, the cradle having an opening on the bottom that exposes the transparent windows of the wells; and an optical imaging module movable across different wells of a same multi-well plate to capture images through the windows.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/32 - Inoculateur ou échantillonneur du type à champs multiples ou en continu
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
69.
SYSTEM AND METHOD FOR VARIABLE SIZE CONTAINER HANDLING
In some examples, a system may include at least two conveyors disposed at a specified distance apart and movable toward each other to reduce the specified distance or away from each other to increase the specified distance. The at least two conveyors may be operable to move a container that is contiguously disposable between the at least two conveyors along a first direction when the at least two conveyors are operated in the first direction and along a second direction that is opposite to the first direction when the at least two conveyors are operated in the second direction.
B65G 15/14 - Transporteurs comportant des surfaces porteuses de charges sans fin, c.-à-d. des tapis roulants ou autres systèmes équivalents, auxquelles l'effort de traction est transmis par des moyens différents des éléments d'entraînement sans fin de même configuration comprenant plusieurs surfaces sans fin travaillant ensemble et à axes parallèles longitudinaux, ou une multiplicité d'éléments parallèles, p. ex. des cordes formant une surface sans fin avec plusieurs courroies sans fin la charge étant transportée entre les courroies
70.
SYSTEM AND METHOD FOR VARIABLE SIZE CONTAINER HANDLING
In some examples, a system may include at least two conveyors disposed at a specified distance apart and movable toward each other to reduce the specified distance or away from each other to increase the specified distance. The at least two conveyors may be operable to move a container that is contiguously disposable between the at least two conveyors along a first direction when the at least two conveyors are operated in the first direction and along a second direction that is opposite to the first direction when the at least two conveyors are operated in the second direction.
B65C 9/04 - Dispositifs pour déplacer les objets, p. ex. des réceptacles, devant le poste d'étiquetage dotés de moyens pour faire tourner les objets
B65C 3/08 - Pose d'étiquettes sur des réceptacles courts et rigides sur le corps du réceptacle
B65G 15/14 - Transporteurs comportant des surfaces porteuses de charges sans fin, c.-à-d. des tapis roulants ou autres systèmes équivalents, auxquelles l'effort de traction est transmis par des moyens différents des éléments d'entraînement sans fin de même configuration comprenant plusieurs surfaces sans fin travaillant ensemble et à axes parallèles longitudinaux, ou une multiplicité d'éléments parallèles, p. ex. des cordes formant une surface sans fin avec plusieurs courroies sans fin la charge étant transportée entre les courroies
B65G 21/14 - Châssis ou carters de support ou de protection pour transporteurs sans fin ou éléments de traction des transporteurs à courroies ou à chaînes mobiles ou à pièces interchangeables ou relativement mobilesDispositifs pour déplacer le châssis ou des parties de celui-ci pour permettre le réglage de la longueur ou du profil des porte-charges ou de l'élément de traction
Methods and compositions are used for evaluating the performance of a mass spectrometer instrument. An oligonucleotide is introduced to the mass spectrometry instrument, the sample is analyzed in negative ionization mode, and the resulting mass spectrometry peaks are compared to known standards of the nucleotide-based sample for purposes of performing a qualification of the mass spectroscopy instrument in negative ionization mode. Compositions are provided for the methods and systems.
In some examples, an apparatus may include an ion injector including an ion injector entrance and an ion injector exit, and a capillary cap disposed at the ion injector exit. The capillary cap may include a capillary cap exit opening that is shaped and sized to reduce gas turbulence after the ion injector exit and improve ion signal stability.
H01J 37/317 - Tubes à faisceau électronique ou ionique destinés aux traitements localisés d'objets pour modifier les propriétés des objets ou pour leur appliquer des revêtements en couche mince, p. ex. implantation d'ions
The disclosure provides methods for aligning a portion of a first image of a tissue with a portion of a second image of the tissue by obtaining one or more local transformations based on the portion of the first image and/or the portion of the second image, wherein each of the one or more local transformations is: (i) for aligning a patch of the first image to a patch of the second image matching the patch of the first image, and (ii) associated with the patch of the first image and/or with the patch of a second image; and determining a transformation for aligning the portion of the first image to the portion of the second image according to the one or more local transformations. Moreover, a corresponding device and software implementing functionality of the methods is provided.
In alternative embodiments, provided are non-natural synthetic antibodies capable of specifically binding a human CD10 polypeptide, or neprilysin, polypeptide. In alternative embodiments, also provided are products of manufacture and kits comprising antibodies as provided herein, and methods for making and using antibodies as provided herein, where the antibodies can be used for in vitro diagnostics by immunohistochemistry (IHC). In alternative embodiments, antibodies as provided herein are used in IHC protocols to diagnose a cancer, for example, leukemic cell cancer of pre-B phenotype, acute lymphoblastic leukemia (ALL), angioimmunoblastic T cell lymphoma, Burkitt lymphoma, chronic myelogenous leukemia in blast crisis, diffuse large B-cell lymphoma, hairy cell leukemia, myeloma, precursor B lymphoblastic leukemia or lymphoma, follicular lymphoma, mantle cell lymphoma, precursor T lymphoblastic leukemia or lymphoma, non-hematolymphoid sarcoma, or carcinoma such as renal cell carcinoma or metaplastic breast carcinoma.
G01N 33/574 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour le cancer
C07K 16/28 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains contre des récepteurs, des antigènes de surface cellulaire ou des déterminants de surface cellulaire
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Mass detection systems; instruments for mass spectrometry measurements; liquid chromatography mass spectrometry systems; liquid chromatography mass detection systems; scientific instruments, namely, liquid chromatography mass spectrometers, including liquid delivery system (pump), injection system, autosampler, column compartment, valving, ion source generation, and calibrant delivery systems; downloadable and recorded software for instrument control, instrument tuning, signal collection, data analysis, and reporting of the output of liquid chromatography mass spectrometers; liquid chromatography mass spectrometry detection systems for biological and chemical separations, measurements, screening, targeted analysis, and identification Software as a service (SaaS) services featuring software for instrument control, instrument tuning, signal collection, data analysis, and reporting of the output of liquid chromatography mass spectrometers
09 - Appareils et instruments scientifiques et électriques
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Mass detection systems; instruments for mass spectrometry measurements; liquid chromatography mass spectrometry systems; liquid chromatography mass detection systems; scientific instruments, namely, liquid chromatography mass spectrometers, including liquid delivery system (pump), injection system, autosampler, column compartment, valving, ion source generation, and calibrant delivery systems; downloadable and recorded software for instrument control, instrument tuning, signal collection, data analysis, and reporting of the output of liquid chromatography mass spectrometers; liquid chromatography mass spectrometry detection systems for biological and chemical separations, measurements, screening, targeted analysis, and identification Software as a service (SaaS) services featuring software for instrument control, instrument tuning, signal collection, data analysis, and reporting of the output of liquid chromatography mass spectrometers
The invention relates to a fluid valve (100) for an analyzer (10), comprising: i) a stator component (110) comprising: a first port (150) in a first radius (111) and a plurality of second ports (114) along a second radius (112); and ii) a rotor component (120) comprising: a slot (121) which is coupled to the first port (150), wherein the slot (121) comprises: a tangential part (122) which extends along a segment of the second radius (112). a) the slot (121) also comprises a radial part (125) which extends from the first port (150) to the second radius (112), and/or b) the fluid valve (100) is designed to rotate the rotor component (120) in relation to the stator component (110) in such a way that mixing and/or proportioning fluid, in particular solvent (113, 115), is enabled.
G01N 30/20 - Injection utilisant une valve d'échantillonnage
G01N 30/34 - Contrôle des paramètres physiques du fluide vecteur de la composition du fluide, p. ex. du gradient
F16K 11/074 - Soupapes ou clapets à voies multiples, p. ex. clapets mélangeursRaccords de tuyauteries comportant de tels clapets ou soupapesAménagement d'obturateurs et de voies d'écoulement spécialement conçu pour mélanger les fluides dont toutes les faces d'obturation se déplacent comme un tout comportant uniquement des tiroirs à éléments de fermeture articulés à pivot à faces d'obturation planes
78.
SAMPLE INJECTOR WITH FLOATING NEEDLE SEAT FOR AN ANALYTICAL DEVICE
A sample injector for an analytical device includes a needle seat configured to receive a needle for injecting a sample into an injector path. The needle seat is mounted in a floating manner so that the orientation of the needle seat is aligned automatically with respect to the orientation of the approaching needle. The sample injector may be provided in an analytical device such as a chromatographic device.
An apparatus for containing a three-dimensional cellular material surrounded by a medium that facilitates and maintains the centering of the three-dimensional cellular material throughout an assay is provided. The apparatus includes a well having an open proximal end and a closed distal end. Further, the well defines a compartment having an interior surface and a sample nesting site for containing the three-dimensional cellular material surrounded by the medium. A central indentation is located at the closed distal end of the well, a first concentric lip is located above the central indentation in a y-direction towards the open proximal end of the well, and a second concentric lip is located above the first concentric lip in the y-direction towards the open proximal end of the well. Additionally, the first concentric lip and the second concentric lip define a groove therebetween. A method of forming a three-dimensional cellular material is also provided.
The Board of Trustees of the Leland Stanford Junior University (USA)
Agilent Technologies, Inc. (USA)
Inventeur(s)
Porteus, Matthew H.
Hendel, Ayal
Clark, Joe
Bak, Rasmus O.
Ryan, Daniel E.
Dellinger, Douglas J.
Kaiser, Robert
Myerson, Joel
Abrégé
Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a modified sgRNA to correct a mutation in a target gene associated with the genetic disease.
A magnetic coupling assembly includes a thermal isolation system that isolates the assembly from a heat source such as a pump coupled to a driven side of the assembly, thereby enabling the assembly to operate at a reduced temperature. The system may include a labyrinth seal that reduces convective heat transfer to the assembly. The seal may include a sealing gap defined between a rotating component and a stationary component. The gap is sized to limit gas conductance therethrough. The system may also include one or more spacers positioned to reduce or eliminate conductive heat transfer from one component to another. The assembly may be utilized in a pump for contactless coupling of a motor shaft to a pump shaft.
H02K 49/10 - Embrayages dynamo-électriquesFreins dynamo-électriques du type à aimant permanent
F04C 2/02 - Machines ou pompes à piston rotatif du type à engrènement, c.-à-d. avec un mouvement de translation circulaire des organes coopérants, chaque organe possédant le même nombre de dents ou de parties équivalentes de prise
F04C 18/02 - Pompes à piston rotatif spécialement adaptées pour les fluides compressibles du type à engrènement, c.-à-d. avec un mouvement de translation circulaire des organes coopérants, chaque organe possédant le même nombre de dents ou de parties équivalentes de prise
F04C 29/00 - Parties constitutives, détails ou accessoires de pompes ou d'installations de pompage spécialement adaptées pour les fluides compressibles non couverts dans les groupes
H02K 5/18 - Enveloppes ou enceintes caractérisées par leur configuration, leur forme ou leur construction avec des nervures ou des ailettes pour améliorer la transmission de la chaleur
A capillary array assembly is fabricated by inserting capillaries into respective grooves of a substrate and fixing the capillaries to the substrate. The capillaries are fixed by applying heat and force to the substrate, with a defined displacement of substrate material and for a defined period of time, thereby spreading heated portions of the substrate over the capillaries. After applying the heat and force, capillaries are at least partially embedded by the substrate and may be at least partially exposed to a top outside surface of the substrate by respective slots. The capillary array assembly may be utilized, for example, in a sample analysis instrument such as a capillary electrophoresis (CE) instrument.
The invention relates to a temperature control apparatus (100) for an analysis device (10), having: i) a receiving volume (110) for receiving at least one sample container (130); and ii) a temperature control element (120) which is designed to at least partially control the temperature of the at least one sample container (130) in the receiving volume (110); wherein the temperature control apparatus (100) is designed to switch between: a) a first operating mode for controlling the temperature of the at least one sample container (130) by means of the temperature control element (120), wherein the temperature control element (120) is designed to directly contact the at least one sample container (130) in the first operating mode, wherein the direct contact comprises: forming an integral connection and/or controlling the temperature by means of a temperature control medium (125) which flows through the temperature control element (120); b) a second operating mode for not controlling the temperature of the at least one sample container (130) by means of the temperature control element (120).
In some examples, a three-dimensional printed nanospray interface for mass spectrometry may include a body including a sheath inlet connected to a sheath outlet for passage of sheath liquid to an emitter. The emitter may include a sidewall including a stepped texture on an inner surface of the sidewall.
In some examples, a three-dimensional printed nanospray interface for mass spectrometry may include a body including a sheath inlet connected to a sheath outlet for passage of sheath liquid to an emitter. The emitter may include a sidewall including a stepped texture on an inner surface of the sidewall.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
B33Y 80/00 - Produits obtenus par fabrication additive
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Custom manufacturing services for others in the field of
drugs for therapeutic use in humans, nucleic acids, and
nucleic acid materials; consulting for custom manufacturing
process; technical support services, namely, providing
technical advice related to the custom manufacture of drugs
for therapeutic use in humans, nucleic acids, and nucleic
acid materials. Pharmaceutical drug development services; biochemical
research and analysis, namely, analysis of nucleic acids;
scientific study and research in the field of nucleic acids
and nucleic acid materials; quality management services,
namely, quality evaluation and analysis, quality assurance,
and quality control, in the field of pharmaceutical drugs
for therapeutic use in humans.
87.
IMMUNOHISTOCHEMISTRY (IHC) PROTOCOLS AND METHODS FOR DIAGNOSING AND TREATING CANCER - CLAUDIN 18.2
In alternative embodiments, provided are immunohistochemistry (IHC) methods for determining and scoring reproducibly the extent of expression of the protein Claudin 18.2, in a tissue sample. In alternative embodiments, provided are methods for diagnosing, treating or ameliorating or assessing the risk of recurrence for a cancer or a tumor using an IHC method as provided herein. In alternative embodiments, provided are kits comprising components and instructions for practicing methods as provided herein. The present application describes methods for scoring Claudin 18.2 expression and utilizing the score as a companion or complementary diagnostic or to treat or ameliorate cancer or a tumor.
G01N 33/574 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour le cancer
C07K 16/18 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains
A61K 39/395 - AnticorpsImmunoglobulinesImmunsérum, p. ex. sérum antilymphocitaire
C07K 14/47 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'animauxPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'humains provenant de vertébrés provenant de mammifères
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Custom manufacturing services for others in the field of
drugs for therapeutic use in humans, nucleic acids, and
nucleic acid materials; consulting for custom manufacturing
process; technical support services, namely, providing
technical advice related to the custom manufacture of drugs
for therapeutic use in humans, nucleic acids, and nucleic
acid materials. Pharmaceutical drug development services; biochemical
research and analysis, namely, analysis of nucleic acids;
scientific study and research in the field of nucleic acids
and nucleic acid materials; quality management services,
namely, quality evaluation and analysis, quality assurance,
and quality control, in the field of pharmaceutical drugs
for therapeutic use in humans.
In some examples, an apparatus may include an advection flow structure including a passage to transport, via advection by a gas flow, an ablated sample from an ablation region to an ionization region.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/26 - Spectromètres de masse ou tubes séparateurs de masse
H01J 49/24 - Systèmes à vide, p. ex. maintenant des pressions voulues
The present invention is directed to a sample holder for use in molecular absorption spectroscopy. The sample holder comprises a first surface having a first predetermined geometry. and a second surface having a second predetermined geometry. The first surface is opposite the second surface. The sample holder is configured to hold a measurement sample between the first surface and the second surface such that a distance between the first surface and the second surface defines an optical pathlength of the sample holder. The predetermined geometries of the first surface and the second surface provides a continuously variable cross-section across the sample holder so as to provide a continuous range of optical pathlengths.
G01N 21/31 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique
The present invention relates to methods for construction of pharmamers i.e. vaccine components characterized by their multimerization domain and the attached biologically active molecules, and their use in preparation of vaccines that contains the pharmamers alone or in combination with other molecules. The individual molecules of the construct can be bound to each other or the multimerization domain(s) by covalent or non-covalent bonds, directly or via linkers. The invention further relates to the use of such preparations in vaccine settings aimed to function as preventive/prophylactic or therapeutic vaccines in humans and animals.
A61K 39/21 - Retroviridae, p. ex. virus de l'anémie infectieuse équine
A61K 47/61 - Préparations médicinales caractérisées par les ingrédients non actifs utilisés, p. ex. les supports ou les additifs inertesAgents de ciblage ou de modification chimiquement liés à l’ingrédient actif l’ingrédient non actif étant chimiquement lié à l’ingrédient actif, p. ex. conjugués polymère-médicament l’ingrédient non actif étant un agent de modification l’agent de modification étant un composé organique macromoléculaire, p. ex. une molécule oligomérique, polymérique ou dendrimérique le composé organique macromoléculaire étant un polysaccharide ou l’un de ses dérivés
A61K 47/64 - Conjugués médicament-peptide, médicament-protéine ou médicament-acide polyaminé, c.-à-d. l’agent de modification étant un peptide, une protéine ou un acide polyaminé lié par covalence ou complexé à un agent thérapeutiquement actif
In some examples, an apparatus may include an advection flow structure including a passage to transport, via advection by a gas flow, an ablated sample from an ablation region to an ionization region.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
H01J 49/24 - Systèmes à vide, p. ex. maintenant des pressions voulues
93.
Smartphone display screen or portion thereof with transitional graphical user interface
09 - Appareils et instruments scientifiques et électriques
Produits et services
Computer software, namely, downloadable and recorded
software for collecting, processing, integrating,
organizing, analyzing, visualizing, and communicating
scientific data in the field of mass spectrometry.
Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as a rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12N 9/08 - Oxydoréductases (1.), p. ex. luciférase agissant sur le peroxyde d'hydrogène comme accepteur (1.11)
C12N 9/96 - Stabilisation d'une enzyme par formation d'un adduct ou d'une compositionFormation de conjugaisons d'enzymes
C12Q 1/28 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase une peroxydase
G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
A cell filtration device and method use a negative dielectrophoretic force (nDEP) to repulse cells from pores of an insulating membrane. A cell suspension is introduced to a first fluidic region, and a fluid and other components pass through pores of the insulating membrane to a second fluidic region. Cells are retained in the first fluidic region and can be recovered continuously or at intervals. The cell filtration device and method minimize contact of the insulating membrane by the cells and avoids clogging of pores.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
In alternative embodiments, provided are immunohistochemistry (IHC) methods for determining and scoring reproducibly the extent of expression of the protein Melanoma Associated Antigen Gene-A4 (MAGE-A4), in a tissue sample. In alternative embodiments, provided are methods for diagnosing, treating or ameliorating or assessing the risk of recurrence for a cancer or a tumor using an IHC method as provided herein. In alternative embodiments, provided are kits comprising components and instructions for practicing methods as provided herein. The present application describes methods for scoring MAGE-A4 expression and utilizing the score as a companion or complementary diagnostic or to treat or ameliorate cancer or a tumor.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
C07K 16/30 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains contre des récepteurs, des antigènes de surface cellulaire ou des déterminants de surface cellulaire provenant de cellules de tumeurs
G01N 1/28 - Préparation d'échantillons pour l'analyse
A capillary array assembly (100) includes a capillary array window holder (104) that provides multiple capillary channels. A section of each capillary channel is an open channel (678) that is exposed to excitation light on at least one side of the capillary array assembly (100). Adjacent open channels (678) are separated from each other by window bars (156). Multiple capillaries (108) are disposed in respective capillary channels, such that windows (148) of the capillaries (108) are located in the open channels (678). The window bars (156) block line of sight between adjacent capillary windows (148) to reduce or eliminate cross-talk between adjacent capillaries (108) when making optical-based measurements of the samples. The capillary array assembly (100) may be mounted in a sample analysis system (1800), which may for example be configured to perform capillary electrophoresis on the samples.
A solvent supply system supplies solvent for one or more chromatographic separation systems each including one or more input channels. Each input channel is configured for fluidically coupling with a respective solvent container for supplying the respective input channel with a respective solvent from the respective solvent container. The solvent supply system includes a solvent identification unit configured for providing a channel identification for identifying a specific solvent container to supply a specific input channel of a specific chromatographic separation system, and for assigning the channel identification to the specific solvent container.
B01D 15/20 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives au conditionnement de la matière adsorbante ou absorbante
B01D 15/18 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives aux différents types d'écoulement
100.
DEPARAFFINIZATION OF TISSUE UTILIZING ELECTRIC FIELD
Paraffin-embedded tissue is prepared removing paraffin from the tissue. The paraffin is removed by generating an electric field effective to produce plasma and direct charged species of the plasma to the paraffin, thereby rendering the paraffin responsive to the electric field. The electric field may move the paraffin out from the tissue due to electrostatic force. Movement of the paraffin may be assisted by moving an electrode utilized to generate the electric field relative to the paraffin. Movement of the paraffin also may be assisted by applying a solvent and/or heat energy to the tissue.
