01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Competent cells for gene transfer used in non-clinical
molecular biology and microbiology research and accompanying
multi-well array plates sold as a unit (term considered too
vague by the International Bureau pursuant to Rule 13 (2)
(b) of the Regulations).
Methods for determining an arm aneuploidy score in a tumor sample genome include selectively amplifying nucleic acid sequences at specific locations in the tumor genome using a targeted panel to generate sequence reads. Next, divide the genome locations into segments with homogeneous copy numbers based on log odds of heterozygous SNPs and CNV log ratios of the sequence reads. Identify gain and loss segments relative to a reference copy number and intersecting respective chromosome arms. Compare the cellularities of these segments to a minimum threshold. Retain the longest segment for the arm that meets the minimum cellularity and sum its total bases. Divide this total by the number of bases in the arm to yield a fraction. If this fraction meets a minimum threshold, filter the segment based on fold changes and determine gains or losses. Count the arms with called gains or losses to generate the arm aneuploidy score.
G16B 20/20 - Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
Described herein are various methods and embodiments of a fluid processing system and foam breaker devices for at least reducing foam formation in a container of the fluid processing system. In some embodiments, the foam breaker device includes a mounting hub extending along a longitudinal axis of the foam breaker device. The mounting hub can include a coupling feature for coupling the foam breaker device to a drive shaft of the fluid processing system. The foam breaker device can also include a wall structure extending circumferentially from the mounting hub at an angle relative to the longitudinal axis of the foam breaker device. The wall structure can cause a reduction in the foam volume as a result of at least a part of the wall structure contacting the foam volume.
The present disclosure provides freeze-dried eukaryotic cell culture media, feeds, and supplement formulations that have enhanced stability when stored refrigerated or at room temperature. The present disclosure contemplates freeze-dried eukaryotic cell culture media and supplement compositions having reduced moisture content and enhanced stability when compared to non-freeze-dried eukaryotic cell culture media, feed, and supplement compositions. Additional aspects include methods of freeze-drying eukaryotic cell culture media and supplement compositions to enhance stability. Further encompassed are methods for stabilizing eukaryotic cell culture media, feed, or supplement compositions by freeze-drying. Still further aspects include freeze-dried eukaryotic cell culture media, feed, and supplement compositions that are agglomerated or powered media compositions.
A chromatography system includes a first chromatography column and a panel having a top face and an opposing bottom face, a first cavity being formed on the panel so as to pass through the top face and be encircled by an inner surface. The panel bounds an inlet fluid channel having an end terminating at an inlet opening formed on the inner surface encircling the first cavity so that the inlet fluid channel communicates with the first cavity. The panel also bounds a plurality of first outlet fluid channels each having an end terminating at an outlet opening formed on the inner surface encircling the first cavity so that each of the plurality of first outlet fluid channels communicate with the first cavity, a first one of the plurality of first outlet fluid channels being in fluid communication with the first chromatography column. A first valve is rotatably disposed within the first cavity.
B01D 15/18 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
B01D 15/14 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus
F16K 11/085 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with all movable sealing faces moving as one unit comprising only taps or cocks with cylindrical plug
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Non-ionic surfactants for use in scientific research, laboratory processes, industrial processes, and manufacturing processes; detergents for use in scientific research, laboratory processes, industrial processes, and manufacturing processes; chemical reagents for use in scientific research, product development, and manufacturing processes, other than for medical or veterinary purposes
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Kits consisting primarily of chemical reagents and also including laboratory equipment, namely, microarrays, sold as a unit for scientific research use
17.
DETECTION AND QUANTIFICATION OF PROTEINS AND NUCLEIC ACIDS USING PROXIMITY EXTENSION AND POLYMERASE CHAIN REACTION WORKFLOWS
A workflow for detecting and/or quantifying a target protein and target nucleic acid(s) in a biological sample, includes binding of the target protein using a pair of proximity probes; synthesizing a nucleic acid extended product including the target protein using a polymerase; and thermocycling a reaction mixture including nucleic acid extended product and the target nucleic acid(s) to form amplicons of the nucleic acid extended product and the target nucleic acid(s); and detecting an emission signal from the amplicons of the nucleic acid extended product and of the target nucleic acid(s) using polymerase chain reaction (PCR). A first emission signal being used to determine a particle titer, and a second emission signal, being used to determine a genome titer. The method may be used for determining the ratios of capsids with full genomes, partially filled capsids and empty capsids, e.g. AAV capsids.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
A device, apparatus, and method for performing an auto-focusing search by generating, while a well is positioned at a first position, a first calibration image showing a fiducial of the well and generating, while the well is positioned at a second position, a second calibration image showing the fiducial, selecting, for a second auto-focusing search, the first position instead of the second position based at least in part on the first calibration image showing a better image focus for the fiducial relative to the second calibration image and performing the second auto-focusing search in a positional region defined based on the first position. The second auto-focusing search performed by generating, while the well is positioned at a third position within the positional region, a third calibration image showing the fiducial, and determining a target position for the well and determining a genetic property of a sample contained in the well.
Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are SIR dyes comprising at least one vinyl group attached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of SiR compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples.
C07F 7/08 - Compounds having one or more C—Si linkages
C07F 9/6561 - Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing systems of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring or ring system, with or without other non-condensed hetero rings
C12Q 1/6816 - Hybridisation assays characterised by the detection means
Compositions for detecting the presence of monkeypox virus in a biological sample includes a monkeypox nucleic acid forward primer; a monkeypox nucleic acid reverse primer; and a monkeypox nucleic acid probe. Methods include a multiplex assay including a three-channel panel with a single assay per channel, that includes a monkeypox assay with a reporter and a quencher, a non-variola orthopox assay with a reporter and a quencher, and a RNase P assay with a reporter and a quencher. A kit for detecting monkeypox virus nucleic acid includes the assay components including the forward and reverser primers and probes for each of the three assays in the multiplex assay.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
A method for compressing molecular tagged sequence data includes: grouping sequence reads associated with a molecular tag sequence to form a family of sequence reads, corresponding vectors of flow space signal measurements and corresponding sequence alignments, calculating an arithmetic mean of the corresponding vectors of flow space signal measurements to form a vector of consensus flow space signal measurements, calculating a standard deviation of the corresponding vectors of flow space signal measurements to form a vector of standard deviations, determining a consensus base sequence based on the vector of consensus flow space signal measurements, determining a consensus sequence alignment and generating a compressed data structure comprising consensus compressed data, the consensus compressed data including for each family, the consensus base sequence, the consensus sequence alignment, the vector of consensus flow space signal measurements, the vector of standard deviations and the number of members.
G16B 40/00 - ICT specially adapted for biostatisticsICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
G16B 40/10 - Signal processing, e.g. from mass spectrometry [MS] or from PCR
G16B 50/00 - ICT programming tools or database systems specially adapted for bioinformatics
H03M 7/30 - CompressionExpansionSuppression of unnecessary data, e.g. redundancy reduction
Cell culture media, concentrated media and feeds, methods of manufacturing cell culture media and feeds, and methods of culturing cells are provided. One or more small peptides, including dipeptides are added to the cell culture media to provide improved stability and improved conditions for culturing cells.
A fluidic device for performing isotachophoresis comprise a sub-strate defining a fluidic network comprising a plurality of inlet channels, the outlets of the inlet channels meeting at a juncture, an intermediate channel extending from the juncture and fluidically coupled to the outlets of the plurality of inlet channels, a first reservoir at the juncture and fluidically coupled to the inlet channels and the intermediate channel, a second reservoir fluidically coupled to the intermediate channel at a location downstream of the juncture, wherein the inlet channels, the collection channel, and the intermediate channel are co-planar, and wherein the first reservoir and second reservoir are configured to produce substantially equal pressure heads. Methods of loading the fluidic device for isotachophoresis can be sequentially through the various inlet channels.
Disclosed herein are compounds that can be used as surfactants for various applications. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are methods of making and using the disclosed compounds.
C07C 43/205 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring the aromatic ring being a non-condensed ring
C07C 47/575 - Compounds having —CHO groups bound to carbon atoms of six-membered aromatic rings containing ether groups, groups, groups, or groups
C07C 323/11 - Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and singly-bound oxygen atoms bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
C08G 65/00 - Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
C07C 43/23 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing hydroxy or O-metal groups
29.
NON-IONIC SURFACTANTS AND METHODS OF MAKING AND USING THE SAME
Disclosed here are compounds that can be used as surfactants for various applications. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are methods of making and using the disclosed compounds.
C07C 43/205 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring the aromatic ring being a non-condensed ring
C07C 43/23 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing hydroxy or O-metal groups
C07C 47/575 - Compounds having —CHO groups bound to carbon atoms of six-membered aromatic rings containing ether groups, groups, groups, or groups
C07C 323/11 - Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and singly-bound oxygen atoms bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
C08G 65/00 - Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
30.
NON-IONIC SURFACTANTS AND METHODS OF USING THE SAME
Disclosed herein are aspects of a compound according to Formula I. The compounds are useful as surfactants and can be used in applications for which a surfactant is indicated. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are aspects of a method for making the compounds and methods for using the compounds in cell assays.
Disclosed herein are compounds that can be used as surfactants for various applications. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are methods of making and using the disclosed compounds.
C07C 43/23 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing hydroxy or O-metal groups
C07C 43/205 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring the aromatic ring being a non-condensed ring
C07C 47/575 - Compounds having —CHO groups bound to carbon atoms of six-membered aromatic rings containing ether groups, groups, groups, or groups
C07C 323/11 - Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and singly-bound oxygen atoms bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
C08G 65/00 - Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
32.
NON-IONIC SURFACTANTS AND METHODS OF USING THE SAME
The present disclosure provides surfactant-based compositions, methods, kits, and systems for use in cell permeabilization, analytical and diagnostic assays, polypeptide stabilization, viral inactivation, and bioprocess workflows. In certain aspects, permeabilization buffers comprising defined concentrations of nonionic surfactants in polar protic solvents permeabilize diverse cell types while preserving intracellular targets and compatibility with downstream detection chemistry and nucleic acid amplification. Related compositions include loading, blocking, running, and mountant buffers, as well as polypeptide stabilization and viral inactivation solutions formulated with the surfactants. In additional aspects, the surfactants are used in lateral flow devices, bead-based assays, and lipid nanoparticle workflows, including determination of encapsulation efficiency. The disclosure further provides methods and systems in which data generated from surfactant-containing workflows are analyzed by machine-learning processes to determine attributes of buffer performance and recommend protocol parameters.
Disclosed herein are aspects of a compound according to Formula (I). tHe compounds are useful as surfactants and can be used in applications for which a surfactant is indicated. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are aspects of a method for making the compounds and methods for using the compounds in cell assays.
Disclosed herein are compounds that can be used as surfactants for various applications. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are methods of making and using the disclosed compounds.
C07C 43/205 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring the aromatic ring being a non-condensed ring
C07C 47/575 - Compounds having —CHO groups bound to carbon atoms of six-membered aromatic rings containing ether groups, groups, groups, or groups
C07C 43/23 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing hydroxy or O-metal groups
C07C 235/44 - Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring
35.
NON-IONIC SURFACTANTS AND METHODS OF USING THE SAME
Disclosed herein are compounds that can be used as surfactants for various applications. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are methods of making and using the disclosed compounds.
C07C 43/205 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring the aromatic ring being a non-condensed ring
C07C 47/575 - Compounds having —CHO groups bound to carbon atoms of six-membered aromatic rings containing ether groups, groups, groups, or groups
C07C 43/23 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing hydroxy or O-metal groups
C07C 235/44 - Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring
36.
NON-IONIC SURFACTANTS AND METHODS OF USING THE SAME
Disclosed herein are compounds that can be used as surfactants for various applications. The disclosed compounds are biodegradable and do not form toxic by-products when degraded and can be used as replacements for current surfactants, such as octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Surfactant-based compositions, methods, kits, and systems can be used in cell permeabilization, analytical/diagnostic assays, polypeptide stabilization, viral inactivation, and bioprocess workflows. Permeabilization buffers comprising the compounds, permeabilize diverse cell types while preserving intracellular targets and are compatible with a variety of downstream detection and analysis methods. Compositions include loading, blocking, running, mountant buffers, polypeptide stabilization and viral inactivation solutions. The surfactants are used in lateral flow devices, bead-based assays, and lipid nanoparticle workflows. The disclosure further provides methods and systems in which data generated from surfactant-containing workflows are analyzed by machine-learning processes to determine attributes performance and recommend protocol parameters.
C07C 43/205 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring the aromatic ring being a non-condensed ring
C07C 47/575 - Compounds having —CHO groups bound to carbon atoms of six-membered aromatic rings containing ether groups, groups, groups, or groups
C07C 323/11 - Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and singly-bound oxygen atoms bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
C08G 65/00 - Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
C07C 43/23 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing hydroxy or O-metal groups
37.
NON-IONIC SURFACTANTS AND METHODS OF USING THE SAME
The present disclosure provides surfactant-based compositions, methods, kits, and systems for use in cell permeabilization, analytical and diagnostic assays, polypeptide stabilization, viral inactivation, and bioprocess workflows. In certain aspects, permeabilization buffers comprising defined concentrations of nonionic surfactants in polar protic solvents permeabilize diverse cell types while preserving intracellular targets and compatibility with downstream detection chemistry and nucleic acid amplification. Related compositions include loading, blocking, running, and mountant buffers, as well as polypeptide stabilization and viral inactivation solutions formulated with the surfactants. In additional aspects, the surfactants are used in lateral flow devices, bead-based assays, and lipid nanoparticle workflows, including determination of encapsulation efficiency. The disclosure further provides methods and systems in which data generated from surfactant-containing workflows are analyzed by machine-learning processes to determine attributes of buffer performance and recommend protocol parameters.
C07C 43/205 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring the aromatic ring being a non-condensed ring
C07C 47/575 - Compounds having —CHO groups bound to carbon atoms of six-membered aromatic rings containing ether groups, groups, groups, or groups
C07C 43/23 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing hydroxy or O-metal groups
C07C 235/44 - Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring
38.
COMPOSITION, SYSTEM, AND METHOD FOR ADENO-ASSOCIATED VIRUS (AAV) PRODUCTION
Compositions, systems, kits and methods for producing AAV vectors in a suspension at high viral titer and purity. Certain aspects include one or more of media, cells, transfection reagent, viral production enhancer, host cell DNA encapsidation reducer, and lysis buffer, each of which is designed to provide maximal viral particle production with high purity from culture of eukaryotic cells, such as 293 cells and insect cells.
Disclosed herein are aspects of a compound according to Formula I
Disclosed herein are aspects of a compound according to Formula I
Disclosed herein are aspects of a compound according to Formula I
The compounds are useful as surfactants and can be used in applications for which a surfactant is indicated. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are aspects of a method for making the compounds and methods for using the compounds in cell assays.
Disclosed herein are compounds that can be used as surfactants for various applications. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are methods of making and using the disclosed compounds.
C09K 23/42 - Ethers, e.g. polyglycol ethers of alcohols or phenols
C07C 43/205 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring the aromatic ring being a non-condensed ring
C07C 43/23 - Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing hydroxy or O-metal groups
C07C 235/48 - Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring having the nitrogen atom of at least one of the carboxamide groups bound to an acyclic carbon atom of a hydrocarbon radical substituted by singly-bound oxygen atoms
C08G 65/334 - Polymers modified by chemical after-treatment with organic compounds containing sulfur
Disclosed herein are compounds that can be used as surfactants for various applications. The disclosed compounds are biodegradable and do not form toxic by-products when degraded. As such, the disclosed compounds can be used as replacements for current surfactants, such as current octylphenol ethoxylates (OPEs) and nonylphenol ethoxylates (NPEs) that have toxic effects when degraded. Also disclosed are methods of making and using the disclosed compounds.
Systems and methods for autofocus using artificial intelligence include (i) capturing a plurality of monochrome images over a nominal focus range, (ii) identifying one or more connected components within each monochrome image, (iii) sorting the identified connected components based on a number of pixels associated with each connected component, (iv) generating a focus quality estimate of at least a portion of the sorted connected components using a machine learning module, and (iv) calculating a target focus position based on the focus quality estimate of the evaluated connected components. The calculated target focus position can be used to perform cell counting using artificial intelligence, such as by (i) generating a seed likelihood image and a whole cell likelihood image based on output—a convolutional neural network and (ii) generating a mask indicative quantity and/or pixel locations of objects based on the seed likelihood image.
A method for biological sample analysis includes receiving reference nucleic acid sequence information and biological sample nucleic acid sequence data, comparing the biological sample nucleic acid sequence data to the reference nucleic acid sequence information, outputting overall consensus metrics of the biological sample nucleic acid sequence data and the reference nucleic acid sequence information based on the comparison and outputting one or more region level consensus metrics of one or more regions of each of the biological sample nucleic acid sequence data and the reference nucleic acid sequence information.
THERMO FISHER ELECTRON SCIENTIFIC INSTRUMENTS LLC (a Delaware corporation) (USA)
LIFE TECHNOLOGIES CORPORATION (a Delaware corporation) (USA)
Inventor
Irwin, Julian James
Smith, Wesley Glen
Abstract
Methods and apparatuses dynamic range enhancement of fluorescence detectors are described herein. In one aspect, a method can include collecting, from a sample during a sample event, an emission spectrum that comprises a plurality of emission signals; based on reception times associated with emission signals of the plurality of emission signals, determining a lifetime estimation for the sample; determining, based at least on the lifetime estimation, a pileup factor affecting receiving emissions from the sample; and based on the determined pileup factor, adjusting a number of emission signals measured during the sample event to generate an estimated number of emissions for the sample event.
Described herein are methods for separating analytes by applying an electric field to one or more samples in a liquid or semi-liquid separation matrix shared by the one or more samples.
The present invention relates to a system for detecting a defect in a fluid-filled specimen. The system includes a fluid conveying device in fluid communication with the specimen to fill it with a fluid. A first electrode is disposed outside the specimen, and a portion of a second electrode is disposed inside the specimen. Further, a detector assembly is in electrical communication with the first and second electrodes, and the detector assembly is configured to detect the defect in the specimen.
The present invention relates to a system for testing integrity of a test specimen, comprising: a housing having a compartment; and a test assembly disposed within the compartment of the housing, the test assembly including, a test platform including a first electrode and an electrode pair, wherein the electrode pair includes a second electrode and a third electrode, the second electrode being coupled to a top end of the test platform; a guard lid configured to cover the top end of the test platform, and the third electrode being coupled to a bottom end of the guard lid; and a detector assembly in electrical communication with the first electrode and the electrode pair, the detector assembly being configured to check the integrity of the test specimen by monitoring an electrical circuit between the first electrode and the electrode pair.
Methods for evaluating microsatellite instability (MSI) analyze nucleic acid sequence reads corresponding to a plurality of marker regions for MSI. The marker regions may include long homopolymers and/or short tandem repeats (STRs). For a target homopolymer, a histogram of homopolymer signal values is calculated based on flow space signal measurements for the homopolymer region in the sequence reads. A score per marker based on features of the histogram of homopolymer signal values is determined for each marker region corresponding to the target homopolymers. For a target STR, the method includes calculating a histogram of repeat lengths for sequence reads corresponding to the marker region of the target STR. A score per STR marker is calculated based on features of the histogram of repeat lengths. A plurality of per marker scores may be combined to form a total MSI score for the sample.
C12Q 1/6809 - Methods for determination or identification of nucleic acids involving differential detection
C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
G16B 20/00 - ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
Described herein are automated capping and de-capping systems and methods of use thereof. Automated capping and de-capping systems include a rack-support unit operable to support at least two types of a rack, a head unit comprising a plurality of cap-drivers, and a drive system. Each rack is operable to support a plurality of tubes having caps, the caps being a first cap type or a second cap type. The second cap type is different than the first cap type. The cap-drivers include a first interface portion operable to engage with the first cap type and a second interface portion operable to engage with the second cap type. The drive system is for moving the rack-support unit and the head unit relative to each other to facilitate engagement between the cap-drivers and the caps of the tubes to cap or de-cap the tubes.
B65B 7/28 - Closing semi-rigid or rigid containers or receptacles not deformed by, or not taking-up shape of, contents, e.g. boxes or cartons by applying separate preformed closures, e.g. lids, covers
55.
QUANTIFICATION OF TARGETS USING CYCLE-BY-CYCLE DATA COLLECTED ON A DIGITAL POLYMERASE CHAIN REACTION PLATFORM
A method for quantification of a target molecule of a biological sample distributed across a plurality of partitions is provided. The method includes receiving fluorescent emission data generated by a polymerase chain reaction (PCR) for each partition of a set of partitions and identifying a partition quantity metric for each partition in the set of partitions to generate a set of partition quality metrics. Each partition quantity metric correlates to a number of target molecules within its respective partition based on the fluorescent emission data. The set of partition quantity metrics is used to determine a state of target molecule amplification within each partition of the set of partitions. The method further includes determining a quantity of the target molecule in the biological sample based on the set of partition quantity metrics.
A bioproduction mixing system includes a flexible compartment having a first end, a second end, and a sidewall extending therebetween, a center axis passing through the flexible compartment between the first end and the second end. A helical assembly is disposed within the flexible compartment and is suspended between the first end and the second end, the helical assembly being offset from the center axis of the flexible compartment.
C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
B01F 23/233 - Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements
B01F 27/054 - Deformable stirrers, e.g. deformed by a centrifugal force applied during operation
B01F 27/114 - Helically shaped stirrers, i.e. stirrers comprising a helically shaped band or helically shaped band sections
B01F 27/213 - Mixers with rotary stirring devices in fixed receptaclesKneaders characterised by their rotating shafts characterised by the connection with the drive
B01F 27/90 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with paddles or arms
B01F 27/92 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with helices or screws
Described herein are automated capping and de-capping systems and methods of use thereof. Automated capping and de-capping systems include a rack-support unit operable to support at least two types of a rack, a head unit comprising a plurality of cap-drivers, and a drive system. Each rack is operable to support a plurality of tubes having caps, the caps being a first cap type or a second cap type. The second cap type is different than the first cap type. The cap-drivers include a first interface portion operable to engage with the first cap type and a second interface portion operable to engage with the second cap type. The drive system is for moving the rack-support unit and the head unit relative to each other to facilitate engagement between the cap-drivers and the caps of the tubes to cap or de-cap the tubes.
Methods and systems for data distribution are described herein. In one aspect, a method can include receiving, by at least one worker node (WN) 110 of a plurality of WNs of a data distribution system, a job definition from a job manager, wherein the job definition comprises a set of processes to be performed on a set of data request; sending, by the WN 110 and to an extended binary access manager (BAMEx) 140, a request for the data; receiving, by the WN 110 and from the BAMEx 140, the data based on the request for the data; performing, by the WN 110, one or more processes according to the job definition; generating, by the WN 110, a set of results files comprising results of at least one of the performed one or more processes; and sending, by the WN 110, the set of results files to the BAMEx 140.
A system includes a central administration module, a biological sample assay instrument, and a biological sample analysis module communicatively coupled to each other, where the central administration module may include: at least one processor; and at least one memory, where the at least one memory contains instructions configuring the at least one processor to: store an instrument user profile and an analysis user profile in a data store; verify a user of the biological sample assay instrument based on the stored instrument user profile; and verify a user of the biological sample analysis module based on the stored analysis user profile. Systems and methods for biological sample analysis include elements for enabling multi-platform authentication and sample analysis auditing.
G16H 40/63 - ICT specially adapted for the management or administration of healthcare resources or facilitiesICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for local operation
G16H 40/67 - ICT specially adapted for the management or administration of healthcare resources or facilitiesICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for remote operation
64.
SYSTEMS AND METHODS FOR MULTIPLEXED POLYMERASE CHAIN REACTION PROCESSES AND DATA ANALYSIS
Systems and methods that enable analyte detection in a multiplexed amplification process can include obtaining, at multiple time points during the amplification process, composite fluorescence signal data associated with a composite fluorescence signal from at least a first probe type comprising a first fluorophore and a second probe type comprising a second fluorophore which has substantially overlapping spectral characteristics as said first fluorophore, the first probe type and the second probe type differing in thermal and/or temporal properties; and determining, based at least partially on the composite fluorescence signal data, fluorescence signal data associated with a fluorescence signal from a given probe type of the first probe type or the second probe type during the amplification process.
Provided herein are, inter alia, compositions and methods useful for the in vivo delivery of bioactive agents (e.g., therapeutic or diagnostic agents). The compositions provided herein include cationic lipids, helper lipids and a biostability enhancing agent, which together form a lipid aggregate with the bioactive agent and allow for the systemic delivery of the bioactive agent to, for example, spleen tissue without the requirement for biomolecular targeting.
A61K 47/69 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
A61K 9/00 - Medicinal preparations characterised by special physical form
A61K 9/1272 - Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers comprising non-phosphatidyl surfactants as bilayer-forming substances, e.g. cationic lipids or non-phosphatidyl liposomes coated or grafted with polymers
A61K 31/7105 - Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
A61K 31/713 - Double-stranded nucleic acids or oligonucleotides
A61K 47/54 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
A61P 11/00 - Drugs for disorders of the respiratory system
C12N 15/88 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using liposome vesicle
A system includes a fluidic system configured to flow a plurality of reagent solutions; an imaging apparatus in communication with the fluidic system, the imaging apparatus comprising an array of image sensors, an array of wells disposed over the array of image sensors, and a flow cell structure defining a flow space over the array of wells; and a data collection circuitry to receive light signal data from the imaging apparatus.
Methods and compositions for purifying a nucleic acid preparation. The methods include contacting a nucleic acid preparation including single-stranded ribonucleic acid (ssRNA) with a double-stranded ribonucleic acid binding protein (dsRBP) that specifically binds to double-stranded ribonucleic acid (dsRNA) and is optionally immobilized on a solid support before or after the dsRBP-dsRNA complex is formed to remove dsRNA from a mixed nucleic acid preparation.
The application describes bioreactors comprising gas permeable membranes disposed on the housing covering at least partially a transfer opening. The bioreactor is also provided with a mixing element and ports adapted to receive sensors measuring values of parameters related to the process implemented in the bioreactor. The bioreactor comprises at least one opening in one of the walls. The at least one opening is covered with a gas permeable membrane.
Provided herein are methods and systems of determining an optimal amplification cycle number during an amplification reaction of a template with unknown concentrations, thereby generating an amplification output within a narrow concentration range and desired quantities regardless of its initial template input. The optimal amplification cycle number can be determined based on a transition point corresponding to a change in signals of the amplification reaction. The transition point can be determined based on applying a derivative to the signals of the amplification, determining a baseline of the signals, or a combination thereof.
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Provided herein are methods and systems of determining an optimal amplification cycle number during an amplification reaction of a template with unknown concentrations, thereby generating an amplification output within a narrow concentration range and desired quantities regardless of its initial template input. The optimal amplification cycle number can be determined based on a transition point corresponding to a change in signals of the amplification reaction. The transition point can be determined based on applying a derivative to the signals of the amplification, determining a baseline of the signals, or a combination thereof.
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
75.
THERMO-RESPONSIVE POLYMER FOR USE IN BIOLOGICAL APPLICATIONS AND METHOD OF MAKING AND USING THE SAME
Thermo-responsive polymers that can be used to provide extracellular matrices for cell/organoid growth and/or for using in isolating/purifying biomolecules. Also disclosed are methods for making and using the polymers. The thermo-responsive polymers exhibit the ability to transition between different phases with changes in temperature and thus provide the ability to grow cells/organoids and then be easily separated from the cells/organoids without destroying them. The thermo-responsive polymer facilitates isolating biomolecules from contaminants that might be produced in biological assays.
A61K 47/42 - ProteinsPolypeptidesDegradation products thereofDerivatives thereof, e.g. albumin, gelatin or zein
A61K 47/58 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. poly[meth]acrylate, polyacrylamide, polystyrene, polyvinylpyrrolidone, polyvinylalcohol or polystyrene sulfonic acid resin
C08F 2/38 - Polymerisation using regulators, e.g. chain terminating agents
C08F 293/00 - Macromolecular compounds obtained by polymerisation on to a macromolecule having groups capable of inducing the formation of new polymer chains bound exclusively at one or both ends of the starting macromolecule
G01N 33/00 - Investigating or analysing materials by specific methods not covered by groups
76.
SAMPLE PREPARATION COMPOSITIONS, DEVICES, SYSTEMS AND METHODS
The present specification relates to compositions, devices, apparatus, methods, kits and systems for sample preparation (e.g., separation, reduction or removal of small molecules from biomolecules in a sample). Exemplary small molecules that can be separated, reduced or removed have a molecular weight range of <2000 Da. and may include, but are not limited to, dyes, biotin, affinity tags, crosslinkers, reducing agents, labels, nanoparticles, radioactive ligands, mass tags, unreacted molecules and combinations, intermediates and derivatives of the foregoing. Exemplary biomolecules present in a sample, include but are not limited to, proteins, glycoproteins, antibodies, peptides, nucleic acids, polysaccharides, carbohydrates and lipids. Methods, compositions, kits, devices, apparatus and systems of the disclosure may advantageously provide superior separation of small molecule contaminants and additionally reduce time and expenses related to separation of small molecules from larger biomolecules in samples. Biomolecules separated as set forth herein are amenable to better downstream processing.
A method of culturing cells for vaccine production comprises culturing cells in the presence of microcarriers with cell culture media and infecting the cells with a virus. The microcarrier comprises a bead and a coating. The coating comprises a thermo-responsive polymer having a lower critical solution temperature (LCST) of between about 20 °C and about 34 °C. The cells adhere to the coating of the microcarrier at a temperature above the LCST. The microcarrier comprises a polymeric bead, and a hydrophobic polymer. The hydrophobic polymer is a block copolymer which is connected to the bead by a covalent bond or by physical adsorption. The block copolymer comprises at least one hydrophobic block and at least one thermo-responsive block.
A61K 47/42 - ProteinsPolypeptidesDegradation products thereofDerivatives thereof, e.g. albumin, gelatin or zein
A61K 47/58 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. poly[meth]acrylate, polyacrylamide, polystyrene, polyvinylpyrrolidone, polyvinylalcohol or polystyrene sulfonic acid resin
C08F 2/38 - Polymerisation using regulators, e.g. chain terminating agents
C08F 293/00 - Macromolecular compounds obtained by polymerisation on to a macromolecule having groups capable of inducing the formation of new polymer chains bound exclusively at one or both ends of the starting macromolecule
G01N 33/00 - Investigating or analysing materials by specific methods not covered by groups
78.
COMPRESSION COLLAR EXPANDER ASSEMBLY AND METHOD OF USE
Various embodiments of a compression collar expander assembly for expanding a tubular compression collar are described. For example, the compression collar expander assembly can include a guide plate, a spindle plate, and a plurality of carriages movably disposed on a top surface of the guide plate. Each of the plurality of carriages can include a body, a guide projecting from the body, and a die set comprising a plurality of dies. Each die can include a base coupled to a corresponding one of the plurality of carriages and a prong upstanding from the base. In some embodiments, a tapered expander is configured to assist with expanding the plurality of dies.
B21D 39/20 - Tube expanders with mandrels, e.g. expandable
B21D 39/04 - Application of procedures in order to connect objects or parts, e.g. coating with sheet metal otherwise than by platingTube expanders of tubes with tubesApplication of procedures in order to connect objects or parts, e.g. coating with sheet metal otherwise than by platingTube expanders of tubes with rods
79.
OVEN AND GRIPPER DESIGN FOR BIOLOGICAL ANALYSIS INSTRUMENT
A biological analysis system for analyzing a biological sample is provided. The system includes a sample holder including a position identifier set, including a first, second, and third position identifier. The system further includes an oven, and a gripper arm system configured to move a sample holder between functional areas of the biological analysis system. The oven includes an outer housing, an inner housing, and an air gap between the outer housing and the inner housing. The oven further includes a heater configured to supply heat to the biological sample and a heat circulating assembly configured to circulate heated air within the housing around the biological samples. The gripper arm system includes an optical sensor configured to measure the position of each position identifier and a processor for determining an x, y, and z position of each position identifier to generate an adjusted coordinate system for controlling the gripper arm.
A method of installing a flexible bioprocess container in a bioprocessing system having a rigid housing with an interior compartment and extending between a top and an opposing base. The method includes coupling a first surface of the flexible bioprocess container to a moveable platform positioned within the interior compartment of the rigid housing; coupling a second surface of the flexible bioprocess container to the base of the rigid housing; and moving the movable platform relative to the rigid housing and toward the top of the rigid housing so as to at least partially expand the flexible bioprocess container within the interior compartment of the rigid housing.
C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
B01F 23/233 - Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements
B01F 27/054 - Deformable stirrers, e.g. deformed by a centrifugal force applied during operation
B01F 27/114 - Helically shaped stirrers, i.e. stirrers comprising a helically shaped band or helically shaped band sections
B01F 27/213 - Mixers with rotary stirring devices in fixed receptaclesKneaders characterised by their rotating shafts characterised by the connection with the drive
B01F 27/90 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with paddles or arms
B01F 27/92 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with helices or screws
A method for aligning an optical imaging sensor for an epifluorescence microscope is provided. The method includes receiving an image of a target pattern of an alignment target positioned near a microscope objective lens. The method further includes determining an image quality for each element of the target pattern and generating a heatmap for the image based on the determined image quality for each element of the target pattern. The heatmap indicates focusing quality and variation over a field-of-view of the objective lens. The method further includes determining focusing metrics based on fitting the heatmap to a distribution profile, and providing the focusing metrics to a user, where the focusing metrics are used to adjust alignment of the optical imaging sensor to improve image quality.
Methods and system are described for normalizing and creating correction factors for measurements in a fluorometer. To ensure that measurements across multiple channels can be properly compared, some kind of calibration must be done. The same calibrant can be run across all the channels and fluorescence measured. The fluorescence of one channel can be chosen as a reference. A correction factor can be calculated for each channel and used for an extended period of time, possibly even the lifetime of the instrument.
G01N 33/96 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving blood or serum control standard
83.
SYSTEMS, METHODS, AND ASSEMBLIES FOR CELL PROCESSING
Functionally closed integrated system for performing a cell processing assay, a consumable assembly for use therewith, and methods of processing cells to perform an assay, such as a cell isolation, cell de-beading, or cell electroporation assay. The integrated system includes a counterflow centrifugation subsystem fluidically coupled to, and integrated with a bead processing subsystem, and optionally an electroporation subsystem. The counterflow centrifugation subsystem and the bead processing subsystem are contained in a unitary housing having a mounting surface with a valve assembly for receiving a consumable assembly, a rotating motor head operable to apply a rotational force to a separation chamber of the consumable assembly, a pump, an electromagnet, a rocker assembly, a central processor operable to control automation of the system, and optionally one or more of, a temperature control unit, and/or sensors for detecting a parameter of fluid within a fluid path of the consumable assembly.
Disclosed herein are scientific instrument support systems, related methods, computing devices and computer-readable media. A method of mitigating distortion of an optical emission spectrum obtained from an optical emission spectrometer is provided. The method may comprise a step of obtaining a spectrum recorded with the spectrometer and a respective one or more condition parameters indicative of an operating condition at a time of recording the spectrum. The method may further comprise a step of providing a model configured to output, in response to the one or more condition parameters, one or more transform parameters of a transformation to be applied to the obtained spectrum. A transformation may be applied in accordance with the obtained one or more transform parameters to the obtained spectrum to mitigate distortion of the spectrum due to a discrepancy between the operating condition and a baseline operating condition.
Provided herein are, inter alia, methods for preparing a liquid cell culture media that has lesser lot-to-lot analytical variation, increased performance, and has lesser metal ion concentrations compared to a liquid media prepared by traditional methods. Such liquid media may be used for culturing cells, including but not limited to, recombinant cells.
Optical systems and methods for collecting light use a collection lens system. An objective lens component of the collection lens system is configured to collect emitted light from a particle in an interrogation region, after irradiation with light from an illumination source. The objective lens component is configured to collect the light in differing spectral ranges and transmit the collected light in a direction of a z-axis of the lens system. An imaging lens component of the collection lens system is configured to receive the light from the objective lens component and transmit the received light in multiple paths corresponding to the differing spectral ranges to a plane at which a detector array is located. The collection lens system is configured to provide substantially constant magnification over a path of light transmitted through the collection lens system to the plane of the detector array.
A transient protein expression system and kit, a composition for producing a recombinant protein in cultured cells, and a method for producing a recombinant protein in cultured cells. The method includes: transfecting 293 cells in a suspension culture having a high density culture medium with a nucleic acid capable of expressing a recombinant protein; contacting the transfected 293 cells with at least one expression enhancer composition; and culturing the transfected 293 cells in the presence of the at least one expression enhancer composition for a period of time such that the recombinant protein is expressed.
An assembly for gel electrophoresis includes a gel cassette and a comb. The gel cassette includes a retainer plate and a divider plate coupled to form a cavity therebetween. The comb includes an elongated body having a first end and a second end, an intermediate portion connected to the elongated body and extending between the first and second ends, the intermediate portion having a third end adjacent the first end and a fourth end adjacent the second end, and a plurality of teeth extending from the intermediate portion. The plurality of teeth is spaced apart from at least one of the third end and the fourth end. In response to the comb being received in the gel cassette, the intermediate portion is received in the cavity such that the third and the fourth ends are configured to engage an internal edge of the cavity.
An assembly for gel electrophoresis includes a gel cassette and a comb. The gel cassette includes a retainer plate and a divider plate coupled to form a cavity therebetween. The comb includes an elongated body having a first end and a second end, an intermediate portion connected to the elongated body and extending between the first and second ends, the intermediate portion having a third end adjacent the first end and a fourth end adjacent the second end, and a plurality of teeth extending from the intermediate portion. The plurality of teeth is spaced apart from at least one of the third end and the fourth end. In response to the comb being received in the gel cassette, the intermediate portion is received in the cavity such that the third and the fourth ends are configured to engage an internal edge of the cavity.
Water-soluble, fluorescent particles and compositions, kits, and methods of making and using such particles are disclosed. Processes for preparing fluorescent particles and for controlling the size, polydispersity and optical properties of such particles also are provided.
C09K 11/02 - Use of particular materials as binders, particle coatings or suspension media therefor
C09B 67/00 - Influencing the physical, e.g. the dyeing or printing, properties of dyestuffs without chemical reaction, e.g. by treating with solventsProcess features in the making of dyestuff preparationsDyestuff preparations of a special physical nature, e.g. tablets, films
The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.
