Provided herein are methods and systems of determining an optimal amplification cycle number during an amplification reaction of a template with unknown concentrations, thereby generating an amplification output within a narrow concentration range and desired quantities regardless of its initial template input. The optimal amplification cycle number can be determined based on a transition point corresponding to a change in signals of the amplification reaction. The transition point can be determined based on applying a derivative to the signals of the amplification, determining a baseline of the signals, or a combination thereof.
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
2.
THERMO-RESPONSIVE POLYMER FOR USE IN BIOLOGICAL APPLICATIONS AND METHOD OF MAKING AND USING THE SAME
Thermo-responsive polymers that can be used to provide extracellular matrices for cell/organoid growth and/or for using in isolating/purifying biomolecules. Also disclosed are methods for making and using the polymers. The thermo-responsive polymers exhibit the ability to transition between different phases with changes in temperature and thus provide the ability to grow cells/organoids and then be easily separated from the cells/organoids without destroying them. The thermo-responsive polymer facilitates isolating biomolecules from contaminants that might be produced in biological assays.
A61K 47/42 - ProteinsPolypeptidesDegradation products thereofDerivatives thereof, e.g. albumin, gelatin or zein
A61K 47/58 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. poly[meth]acrylate, polyacrylamide, polystyrene, polyvinylpyrrolidone, polyvinylalcohol or polystyrene sulfonic acid resin
C08F 2/38 - Polymerisation using regulators, e.g. chain terminating agents
C08F 293/00 - Macromolecular compounds obtained by polymerisation on to a macromolecule having groups capable of inducing the formation of new polymer chains bound exclusively at one or both ends of the starting macromolecule
G01N 33/00 - Investigating or analysing materials by specific methods not covered by groups
3.
TEMPERATURE-RESPONSIVE MICROCARRIERS FOR ADHERENT CELL CULTURE
A method of culturing cells for vaccine production comprises culturing cells in the presence of microcarriers with cell culture media and infecting the cells with a virus. The microcarrier comprises a bead and a coating. The coating comprises a thermo-responsive polymer having a lower critical solution temperature (LCST) of between about 20 °C and about 34 °C. The cells adhere to the coating of the microcarrier at a temperature above the LCST. The microcarrier comprises a polymeric bead, and a hydrophobic polymer. The hydrophobic polymer is a block copolymer which is connected to the bead by a covalent bond or by physical adsorption. The block copolymer comprises at least one hydrophobic block and at least one thermo-responsive block.
4.
COMPRESSION COLLAR EXPANDER ASSEMBLY AND METHOD OF USE
Various embodiments of a compression collar expander assembly for expanding a tubular compression collar are described. For example, the compression collar expander assembly can include a guide plate, a spindle plate, and a plurality of carriages movably disposed on a top surface of the guide plate. Each of the plurality of carriages can include a body, a guide projecting from the body, and a die set comprising a plurality of dies. Each die can include a base coupled to a corresponding one of the plurality of carriages and a prong upstanding from the base. In some embodiments, a tapered expander is configured to assist with expanding the plurality of dies.
B21D 39/20 - Tube expanders with mandrels, e.g. expandable
B21D 39/04 - Application of procedures in order to connect objects or parts, e.g. coating with sheet metal otherwise than by platingTube expanders of tubes with tubesApplication of procedures in order to connect objects or parts, e.g. coating with sheet metal otherwise than by platingTube expanders of tubes with rods
5.
OVEN AND GRIPPER DESIGN FOR BIOLOGICAL ANALYSIS INSTRUMENT
A biological analysis system for analyzing a biological sample is provided. The system includes a sample holder including a position identifier set, including a first, second, and third position identifier. The system further includes an oven, and a gripper arm system configured to move a sample holder between functional areas of the biological analysis system. The oven includes an outer housing, an inner housing, and an air gap between the outer housing and the inner housing. The oven further includes a heater configured to supply heat to the biological sample and a heat circulating assembly configured to circulate heated air within the housing around the biological samples. The gripper arm system includes an optical sensor configured to measure the position of each position identifier and a processor for determining an x, y, and z position of each position identifier to generate an adjusted coordinate system for controlling the gripper arm.
A method of installing a flexible bioprocess container in a bioprocessing system having a rigid housing with an interior compartment and extending between a top and an opposing base. The method includes coupling a first surface of the flexible bioprocess container to a moveable platform positioned within the interior compartment of the rigid housing; coupling a second surface of the flexible bioprocess container to the base of the rigid housing; and moving the movable platform relative to the rigid housing and toward the top of the rigid housing so as to at least partially expand the flexible bioprocess container within the interior compartment of the rigid housing.
C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
B01F 23/233 - Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements
B01F 27/054 - Deformable stirrers, e.g. deformed by a centrifugal force applied during operation
B01F 27/114 - Helically shaped stirrers, i.e. stirrers comprising a helically shaped band or helically shaped band sections
B01F 27/213 - Mixers with rotary stirring devices in fixed receptaclesKneaders characterised by their rotating shafts characterised by the connection with the drive
B01F 27/90 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with paddles or arms
B01F 27/92 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with helices or screws
A method for aligning an optical imaging sensor for an epifluorescence microscope is provided. The method includes receiving an image of a target pattern of an alignment target positioned near a microscope objective lens. The method further includes determining an image quality for each element of the target pattern and generating a heatmap for the image based on the determined image quality for each element of the target pattern. The heatmap indicates focusing quality and variation over a field-of-view of the objective lens. The method further includes determining focusing metrics based on fitting the heatmap to a distribution profile, and providing the focusing metrics to a user, where the focusing metrics are used to adjust alignment of the optical imaging sensor to improve image quality.
Methods and system are described for normalizing and creating correction factors for measurements in a fluorometer. To ensure that measurements across multiple channels can be properly compared, some kind of calibration must be done. The same calibrant can be run across all the channels and fluorescence measured. The fluorescence of one channel can be chosen as a reference. A correction factor can be calculated for each channel and used for an extended period of time, possibly even the lifetime of the instrument.
G01N 33/96 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving blood or serum control standard
9.
SYSTEMS, METHODS, AND ASSEMBLIES FOR CELL PROCESSING
Functionally closed integrated system for performing a cell processing assay, a consumable assembly for use therewith, and methods of processing cells to perform an assay, such as a cell isolation, cell de-beading, or cell electroporation assay. The integrated system includes a counterflow centrifugation subsystem fluidically coupled to, and integrated with a bead processing subsystem, and optionally an electroporation subsystem. The counterflow centrifugation subsystem and the bead processing subsystem are contained in a unitary housing having a mounting surface with a valve assembly for receiving a consumable assembly, a rotating motor head operable to apply a rotational force to a separation chamber of the consumable assembly, a pump, an electromagnet, a rocker assembly, a central processor operable to control automation of the system, and optionally one or more of, a temperature control unit, and/or sensors for detecting a parameter of fluid within a fluid path of the consumable assembly.
Provided herein are, inter alia, methods for preparing a liquid cell culture media that has lesser lot-to-lot analytical variation, increased performance, and has lesser metal ion concentrations compared to a liquid media prepared by traditional methods. Such liquid media may be used for culturing cells, including but not limited to, recombinant cells.
Optical systems and methods for collecting light use a collection lens system. An objective lens component of the collection lens system is configured to collect emitted light from a particle in an interrogation region, after irradiation with light from an illumination source. The objective lens component is configured to collect the light in differing spectral ranges and transmit the collected light in a direction of a z-axis of the lens system. An imaging lens component of the collection lens system is configured to receive the light from the objective lens component and transmit the received light in multiple paths corresponding to the differing spectral ranges to a plane at which a detector array is located. The collection lens system is configured to provide substantially constant magnification over a path of light transmitted through the collection lens system to the plane of the detector array.
A transient protein expression system and kit, a composition for producing a recombinant protein in cultured cells, and a method for producing a recombinant protein in cultured cells. The method includes: transfecting 293 cells in a suspension culture having a high density culture medium with a nucleic acid capable of expressing a recombinant protein; contacting the transfected 293 cells with at least one expression enhancer composition; and culturing the transfected 293 cells in the presence of the at least one expression enhancer composition for a period of time such that the recombinant protein is expressed.
An assembly for gel electrophoresis includes a gel cassette and a comb. The gel cassette includes a retainer plate and a divider plate coupled to form a cavity therebetween. The comb includes an elongated body having a first end and a second end, an intermediate portion connected to the elongated body and extending between the first and second ends, the intermediate portion having a third end adjacent the first end and a fourth end adjacent the second end, and a plurality of teeth extending from the intermediate portion. The plurality of teeth is spaced apart from at least one of the third end and the fourth end. In response to the comb being received in the gel cassette, the intermediate portion is received in the cavity such that the third and the fourth ends are configured to engage an internal edge of the cavity.
An assembly for gel electrophoresis includes a gel cassette and a comb. The gel cassette includes a retainer plate and a divider plate coupled to form a cavity therebetween. The comb includes an elongated body having a first end and a second end, an intermediate portion connected to the elongated body and extending between the first and second ends, the intermediate portion having a third end adjacent the first end and a fourth end adjacent the second end, and a plurality of teeth extending from the intermediate portion. The plurality of teeth is spaced apart from at least one of the third end and the fourth end. In response to the comb being received in the gel cassette, the intermediate portion is received in the cavity such that the third and the fourth ends are configured to engage an internal edge of the cavity.
Water-soluble, fluorescent particles and compositions, kits, and methods of making and using such particles are disclosed. Processes for preparing fluorescent particles and for controlling the size, polydispersity and optical properties of such particles also are provided.
C09K 11/02 - Use of particular materials as binders, particle coatings or suspension media therefor
C09B 67/00 - Influencing the physical, e.g. the dyeing or printing, properties of dyestuffs without chemical reaction, e.g. by treating with solventsProcess features in the making of dyestuff preparationsDyestuff preparations of a special physical nature, e.g. tablets, films
The method includes compressing numbers of reads data for targeted genes of a gene expression assay performed on a test sample. The targeted genes are organized into categories. Each category represents a functional context associated with the targeted genes in that category. The numbers of reads corresponding to targeted genes each category is compressed to form a compressed value for the category. The compressed value is compared to a baseline value for the category to determine an enrichment or a loss of a signature corresponding to the functional context of the category. The method may include analyzing information from multiple assays performed on the test sample, assigning a score value to each assay result and predicting a response to immune-oncology treatment based on the assigned scores.
The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.
A computer-implemented method for monitoring a biological analysis is provided. The method includes receiving image data of a first portion of a set of reaction sites and determining fluorescence from the image data. The method further includes displaying, on a user interface, a first graphical visualization of determined fluorescence in each reaction site of the first portion and receiving a selection of a subset of reaction sites from the set of reaction sites from a user. The method also includes displaying, on the user interface, in response to the selection, a graphical visualization of the subset of the set of reaction sites, where the graphical visualization of the subset includes an indication of progress of receiving image data in each reaction site of the subset and an indication of determined fluorescence of each reaction site in the subset.
Recombinant nucleic acids, compositions and methods for producing polynucleotides, such as donor sequences, as well as their use in a variety of applications including genome engineering.
Systems and methods that enable analyte detection in a multiplexed amplification process can include obtaining, at multiple time points during the amplification process, composite emission signal data associated with a composite emission signal from at least a first probe type comprising a first label configured to generate a first emission signal and a second probe type comprising a second label configured to generate a second emission signal which has spectrally similar characteristics as said first emission signal. the first probe type and the second probe type differing in thermal and/or temporal properties; and determining, based at least partially on the composite emission signal data, emission signal data associated with a emission signal from a given probe type of the first probe type or the second probe type during the amplification process.
An in vitro method, composition and kit for determining the presence or absence of adenovirus, metapneumovirus, rhinovirus/enterovirus, and parainfluenza in a sample, including providing a reaction mixture containing the sample and at least one primer pair set. The primer pair set includes at least one primer pair A that specifically amplifies a portion of adenovirus genome; at least one primer pair B that specifically amplifies a portion of metapneumovirus genome; at least one primer pair C that specifically amplifies a portion of rhinovirus/enterovirus genome; and at least one primer pair D that specifically amplifies a portion of parainfluenza genome. The reaction mixture is subjected to reaction conditions suitable to amplify targeted nucleic acids, thereby generating at least one amplicon; wherein the presence or absence of at least one amplicon in the sample indicates the presence or absence of adenovirus, metapneumovirus, rhinovirus/enterovirus, and/or parainfluenza in the sample.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
C12Q 1/48 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving transferase
Disclosed are compositions, kits, and methods that enable intra-channel multiplexing by enabling determination of separate detectable signals, each associated with a different assay target, within the same detection channel. The multiple detectable signals can be separately resolved and independently analyzed to enable detection and/or quantification of each respective target. Enabling multiple targets to be assayed within the same detection channel increases the plexy of multiplex assays without relying on additional dyes and concomitant issues of increased spectral overlap.
A system for robotic laboratory operations includes a stationary surface (2) adjacent laboratory equipment and at least one mover (4) configured to perform an action upon a payload atop the stationary surface (2). The action includes but is not limited to translation across at least a portion of the stationary surface. The at least one mover (4) has a drive member (12) configured to drive the translation a carrier that is mounted to the drive member and has a top surface configured to carry a pay load (8). The drive member (12) is configured to drive the at least one mover (4) across the at least the portion of the stationary surface (2) for moving the payload relative to the laboratory equipment.
Purifying target biomolecules, such as nucleic acids or proteins, from a biological source is a time intensive process and is typically performed by a skilled technician or scientist owing to the highly technical nature of the work. Systems, devices, and methods disclosed herein enable the automated bioprocessing and purification of target biomolecules from a biological source. For example, an instrument and disposable cartridge are provided for automatedly isolating and purifying nucleic acids (such as plasmid DNA from a bacterial culture) or for isolating protein from any biological sample. Such an exemplary instrument and cartridge can work in concert to timely release, mix, and move the target biomolecule and various reagents and buffers through a target biomolecule purification process, resulting in a purified target biomolecule with less manual oversight than traditional approaches.
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
Methods and systems for characterization and optimization of flow cytometry voltages are described herein. According to one aspect of the present disclosure, a method can include applying a plurality of voltages to a detector of a flow cytometry system, the detector optionally comprising a photomultiplier tube (PMT); with the detector, collecting emissions of at least one standard caused by exciting the at least one standard, the at least one standard optionally comprising a bead; measuring a robust coefficient of variance (rCV) for intensity levels of the collected emissions across the plurality of applied voltages; identifying, as an operating voltage setting, a voltage setting where the rCV is essentially asymptotic; and setting the applied voltage of the detector to the identified operating voltage setting.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
Goods & Services
Chemicals for use in industry and science; diagnostic
preparations for scientific or research use; DNA polymerase,
reagents and reagent kits comprising generic DNA circle, DNA
polymerase and buffers for scientific, medical or veterinary
research use; DNA polymerase, reagents and reagent kits
comprising generic DNA circle, DNA primers, DNA polymerase
and buffers for use in the biotechnology field. Diagnostic preparations for clinical or medical laboratory
use.
Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. A scientific instrument support apparatus is disclosed comprising generating logic to generate mass spectrum data during a tandem mass tag labeling experiment including a plurality of channels, determining logic to determine, in the generated mass spectrum data, a correction ratio between a first reporter ion peak intensity corresponding to a first non-deuterated tag and a second reporter ion peak intensity corresponding to a first deuterated tag, and normalizing logic to normalize reporter ion intensities corresponding to a second non-deuterated tag and a second deuterated tag based on the determined correction ratio.
A targeted panel with low sample input requirements from a tumor only sample may be processed to estimate mutation load in a tumor sample. The method may include: detecting variants in nucleic acid sequence reads corresponding to targeted locations in the tumor sample genome; annotating detected variants with an annotation information from a population database; filtering the detected variants, wherein the filtering retains the somatic variants and removes germline variants; calculating an initial TMB; and applying a calibration to the initial TMB level to produce a final TMB level for the mutation load of the tumor sample genome. The filtering may also include retaining nonsynonymous SNVs and indels for the analysis.
A targeted panel with low sample input requirements from a tumor sample may be processed to identify the presence of a mutational signature. The method may include the steps of: amplifying nucleic acid sequences at targeted locations in the tumor sample genome by a targeted panel to generate nucleic acid sequence reads, detecting variants in the nucleic acid sequence reads, generating a set of trinucleotides by appending flanking 5′ and 3′ bases to each variant, determining a frequency of each trinucleotide to form a mutation matrix, determining a cosine similarity value of the mutation matrix and each mutational signature in a matrix of mutational signatures to form a matrix of similarity values, and selecting mutational signatures from the matrix of mutational signatures when a corresponding cosine similarity value is greater than or equal to a threshold to indicate presence of the selected mutational signatures in the tumor sample genome.
C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
49.
METHODS FOR ELECTROSCOPIC IMAGING FOR ANALYSIS OF CELLS
Analyzing cells disposed on a sensor array surface of a ChemFET sensor array, may include flowing a solution having a step change in pH across the sensor array surface, wherein ChemFET sensors of the sensor array generate signals in response to the step change in pH to produce electroscopic image data. Multiple frames of the electroscopic image data are acquired during an acquisition time interval. Each frame corresponds to signal samples generated by the sensor array measured at a sampling time during the acquisition time interval. Each frame comprises pixels, wherein a given pixel in the frame corresponds to a signal sample from a given sensor in the sensor array. The electroscopic image data is segmented, based on characteristics of the signal samples, into cell regions corresponding to locations of the cells on the sensor array surface and background regions corresponding to areas on the sensor array having no cells.
Methods and systems for detecting gene level copy numbers for BRCA1 and BRCA2 genes include amplifying a nucleic acid sample in a presence of a primer pool to produce a plurality of amplicons. The primer pool may include target-specific primers targeting regions of exons of the BRCA1 and BRCA2 genes and sample ID regions. Overlapping amplicons cover the exons of the BRCA1 and BRCA2 genes. Sample ID amplicons are generated for targeted sample ID regions. The amplicons are sequenced to produce sequence reads. The sequence reads are mapped to a reference genome. Determining whole gene copy numbers for the BRCA1 and BRCA2 genes is based on the number of reads per amplicon for the amplicons associated with the exons of the BRCA1 and BRCA2 genes, respectively, and the number of reads per amplicon for the sample ID amplicons associated with the sample ID regions.
C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
52.
FLUID RESERVOIR AND CAP ASSEMBLY FOR BIOLOGICAL ANALYSIS INSTRUMENTS
A system for fluid handling is provided. The system includes a housing, and a cap assembly connected to a fluid reservoir, a sliding secure mechanism connected to the housing, and an optical sensor configured to detect if the cap assembly is fully inserted. The cap assembly is configured to fluidically seal the fluid reservoir, and to be removeable from the fluid reservoir. The sliding secure mechanism is configured to receive the cap assembly. The sliding secure mechanism includes a tactile detent to provide mechanical feedback in response to the cap assembly being fully inserted.
Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of SARS-CoV-2, including SARS-CoV-2 variants, or other coronaviruses from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
A method for compressing molecular tagged sequence data includes: grouping sequence reads associated with a molecular tag sequence to form a family of sequence reads, corresponding vectors of flow space signal measurements and corresponding sequence alignments, calculating an arithmetic mean of the corresponding vectors of flow space signal measurements to form a vector of consensus flow space signal measurements, calculating a standard deviation of the corresponding vectors of flow space signal measurements to form a vector of standard deviations, determining a consensus base sequence based on the vector of consensus flow space signal measurements, determining a consensus sequence alignment and generating a compressed data structure comprising consensus compressed data, the consensus compressed data including for each family, the consensus base sequence, the consensus sequence alignment, the vector of consensus flow space signal measurements, the vector of standard deviations and the number of members.
G16B 40/00 - ICT specially adapted for biostatisticsICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
G16B 40/10 - Signal processing, e.g. from mass spectrometry [MS] or from PCR
G16B 50/00 - ICT programming tools or database systems specially adapted for bioinformatics
H03M 7/30 - CompressionExpansionSuppression of unnecessary data, e.g. redundancy reduction
Provided are laser sources, the laser sources comprising at least one diode; and an optic fiber of a predefined length disposed between the laser source and a position for a target such that the optic fiber communicates light pulses from the laser source as a source light to the position for the target, wherein the position is illuminated by the source light so as to reduce speckles in a captured image of the target. Also provided are methods for providing source light for generating an image, comprising: generating illumination with one or more laser diodes; and passing the illumination through an optic fiber having a plurality of bends therein such that source light is emitted from the optic fiber so as to illuminate a target with the source light, the source light reducing speckles in an image of the target.
Various methods are disclosed for amplifying nucleic acid sequences in a nucleic acid sample. The methods involve forming at least five amplification reaction mixes each including an aliquot from a sample source that includes nucleic acid sequences, using at least five different assays each including a pair of amplification primers, the assays selected from the group of assays in Table 1 and/or targeting the sequences specified in Table 1.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/6837 - Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
This specification generally relates to trinucleotide RNA cap analogs, methods of use thereof, and kits comprising same. In particular, the trinucleotide cap analogs provided herein permit ready detection and/or isolation of capped RNA transcripts in vitro and translation of capped mRNAs in vivo.
A61K 47/54 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
A pipette tip holder includes a tray including an array of openings to receive pipette tips. A plurality of the openings provide access to interiors of a plurality of enclosures. At least one opening of the array of openings of the array is free of an enclosure. The pipette tip holder further includes a container. The tray is secured over a mouth of the container. The at least one opening of the array of openings provides access through the tray to the interior of the container.
Systems or techniques are provided for facilitating retrieval augmented generative question and answer boosting. In various embodiments, a system can access a plain text question regarding a scientific instrument. In various aspects, the system can generate, via a large language model that references a document-graph repository, a structured or unstructured answer for the plain text question. In various instances, the document-graph repository can comprise a plurality of document-graphs that respectively correspond to a plurality of technical documents. In various cases, for a first document-graph that corresponds to a first technical document, leaf nodes of the first document-graph can represent respective text blocks written in the first technical document, and non-leaf nodes of the first document-graph can respectively represent a document title, one or more section headings, and one or more scientific instrument identifiers written in the first technical document and beneath which the respective text blocks are nested.
Apparatus, methods, and systems including a syringe for delivering a fluid. The syringe includes a syringe body, a piston, an insert, and a removable plunger. The piston is located in a lumen of the syringe body such that a bottom of the piston together with interior walls of the syringe define a working volume. The piston comprising a cavity. The insert is located at a desired position in the lumen. The insert narrowing the lumen and being configured to prevent retraction of the piston beyond the desired position. The removable plunger is configured to removably couple to the piston. The removable plunger includes a tip configured to couple with the cavity of the piston and move the piston when a force is applied at the plunger, and to decouple from the piston when the piston engages the insert at the desired position.
The present disclosure relates to N-protected NH-rhodanine dyes and their use in nucleic acid detection. In particular, the disclosure relates to methods of making N-protected NH-rhodamine dyes, and methods of use of N-protected NH-rhodamine dyes (e.g., human identification). Certain dyes provided herein have unique spectral properties that complement those in existing dye sets and can be used to expand the number of reporter dyes that can be included for HID applications and other biological assays.
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
Sample preparation compositions and methods for purifying plasmid DNA from biological samples is provided, are provided. The compositions and methods provided herein allow pDNA analysis to be carried out without centrifugation. The preparation process is amenable to high throughput processing using manual or robotic platforms.
Described herein are compositions, methods, and kits for detecting antibiotic resistance genes in a sample, such as a wound swab. One embodiment described herein is primer pairs and probes for individual or multiplex polymerase chain reaction (PCR) based assays for the detection of one or more antibiotic resistance gene targets comprising resistance to molecularly characterized extended-spectrum β-lactamases (MESBLs); extended-spectrum β-lactamases (ESBLs); carbapenemase; AmpC β-lactamase; β-lactamase; lincosamide, macrolide, streptogramin; trimethoprim; macrolides; methicillin; colistin; sulfonamide; tetracycline; vancomycin; nitroimidazole; quinolone; or aminoglycoside.
C12Q 1/6809 - Methods for determination or identification of nucleic acids involving differential detection
G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/6837 - Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
01 - Chemical and biological materials for industrial, scientific and agricultural use
09 - Scientific and electric apparatus and instruments
Goods & Services
Kits consisting primarily of reagents and laboratory
equipment, namely, microarrays sold as a unit for scientific
research use. Laboratory equipment, namely, microarrays.
Provided herein are compositions, methods and uses that relate to or result from providing separation media having at least one flocculant ligand covalently attached to a base surface or support, and the separation and/or purification of biological molecules using the separation media of the present disclosure. Certain embodiments provide separation media which under certain modes of operation and enhance the separation of the molecule of interest from impurities. Embodiments are described, for example, for the separation of plasma protein(s) from impurities from plasma-derived samples using separation media disclosed herein.
B01J 39/05 - Processes using organic exchangers in the strongly acidic form
B01D 15/36 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
B01J 39/20 - Macromolecular compounds obtained by reactions only involving unsaturated carbon-to-carbon bonds
B01J 47/014 - Ion-exchange processes in generalApparatus therefor in which the adsorbent properties of the ion-exchanger are involved, e.g. recovery of proteins or other high-molecular compounds
A method can comprise receiving, at a user interface, at least one user selection associated with cell staining. The method can further comprise determining a first product associated with staining a first portion of a cell. The method can further comprise determining, a first simulated view of a stained cell stained with the first product, wherein the first simulated view of the stained cell comprises a portion of the cell highlighted in a particular color. The method can further comprise determining a first emission spectrum associated with the first product and the particular color. The method can further comprise displaying, at the user interface, at least one of the first simulated view of the stained cell and the first emission spectrum associated with the first simulated view of the stained cell.
Systems and methods for calibrating an imager for unmixing of images with multiple fluorophores is described. One example method performed by a computing device includes receiving one or more single-color control images, a foreground channel, and a background channel. The single-color control images are images of the sample with a single fluorophore applied to the sample, and the foreground channel and the background channel are associated with the fluorophore. The method includes determining a difference between the foreground channel and the background channel, acquiring a foreground pixel mask from the difference, averaging a set of foreground pixels in the foreground pixel mask to generate a first spectrum, and subtracting an unstained spectrum from the first spectrum to generate a second spectrum. The second spectrum defines a spectral profile of the sample. A calibration slide and a method of preparing a calibration slide are also described.
G01N 21/27 - ColourSpectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection
This disclosure generally relates to spatial imaging methods and systems, as well as compositions and kits for use in such methods. Reagents and methods are provided herein that can be used for successful detection using fluorescent and bright-field microscopes and spectral imaging systems of a wide range of various protein markers across numerous types of biological samples.
Disclosed herein is a focusing stage. The focusing stage comprises a unit configured to perform real-time focus correction during imaging of a sample. The sample is contained by a substrate positioned on a sample stage. The unit comprises a multi-spot light module comprising a plurality of stationary light sources, at least one optical light splitter configured to reflect a portion of light projected by at least one first stationary light source of the plurality of stationary light sources, at least one detector configured to detect spots of light reflected from interfaces of the substrate, a detector processor configured to calculate, based on locations of the spots on the at least one detector, a correction of a distance between a first objective lens of a plurality of objective lenses and the substrate, and an actuator configured to adjust the distance between the first objective lens and the substrate by the correction.
An instrument for biological analysis includes a base, an excitation source, an optical sensor, an excitation optical system, and an emission optical system. The base is configured to receive a sample holder comprising a plurality of biological samples. The optical sensor is configured to receive emissions from the biological samples in response to the excitation source. The instrument may additionally include a sensor lens enclosed by a lens case and a focusing mechanism including a gear that engages the lens case, the focusing mechanism being accessible outside the enclosure for adjusting a focus. The instrument may further include a sensor aperture dispose along an emission optical path and a blocking structure disposed to cooperate with the sensor aperture such that none of the reflected radiation from an illuminated surface near the sample holder is received by the optical sensor that does not also reflect off another surface of the instrument.
Described herein are compositions, methods, and systems for the detection and quantification of 5-plex and 6-plex multiplex (5 or 6 amplified nucleic acid targets) with QSY2™ probes. Some embodiments relate to detection and quantification of 5-plex and 6-plex multiplex (5 or 6 amplified nucleic acid targets) with a combination of QSY2™ probes and QSY™ probes, or a combination of QSY2™ probes with MGB probes, in a single reaction. Other embodiments relate to detection and quantification of 5-plex and 6-plex multiplex (5 or 6 amplified nucleic acid targets) with a combination of QSY2™ probes with QSY™ probes, or a combination of QSY2™ probes and MGB probes in a single reaction.
The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods, and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.
The present disclosure is related generally to systems and methods for high level expression of recombinant proteins from baculovirus in insect cells. In particular, the methods and systems described herein allow for high levels of baculovirus production in insect cells and/or high levels of protein production in insect cells using a chemically defined, yeast lysate-free insect cell medium. The disclosure also relates to compositions and kits for culturing, transfecting, and/or producing recombinant protein in insect cells.
Systems and methods for enriching or isolating one or more biomolecules are disclosed herein. Existing biomolecule collection and isolation systems and methods require multiple, sequential processes for pre-processing biomolecules after in vitro production and before final isolation (e.g., using affinity chromatography). Failure to sufficiently pre-process (e.g., purify) biomolecules prior to isolation using affinity chromatography frequently results in failure of chromatography equipment, unacceptable decreases in contaminant breakthrough, process throughput, and reagent usage, and increased likelihood of sample contamination or degradation. Systems and methods are disclosed herein that eliminate one or more steps of existing biomolecule isolation systems and protocols, which can decrease time and cost of purification or isolation of biomolecules of interest while maintaining acceptable yield and purity parameters.
A system includes a pipetting system including a 3-axis gantry; a sled mechanism to select a magnetic comb from a set of magnetic combs; a fluorometer; and a set of receptacles to receive welled plates. A method for purifying nucleic acids includes applying a sample to a well of a multi-well plate, selecting a magnetic comb from a set of magnetic combs disposed on a gantry system, collecting magnetic beads using the magnetic comb, collecting nucleic acid using the magnetic beads, and eluting the nucleic acid from the beads.
Various embodiments of the present invention comprise a supply chain platform configured in connection with an inventory hub, a supplier system, an enterprise data platform, and a customer system to respond to disturbance events within a supply chain. Such a supply chain platform may collect a variety of supply chain data to determine a risk score relating to such disturbance event(s) and one or more mitigation opportunities in response thereto via a mitigation module configured to generate one or more scenarios and assess the same for efficacy and readiness. Such mitigation opportunities may include the use of one or more supplier sites within the supply chain. Such a supply chain platform may be configured to generate a plurality of reports having interactive data visualizations configurable according to key element data and/or parameter input(s) supplied by a user through a user device and may differentiate between important and inconsequential disturbance events.
Described herein is a tissue sample compartmentalization apparatus and methods for labeling and identifying one or more analytes in a tissue sample. In one embodiment the apparatus comprises a tissue sample compartmentalization module having a first member defining a first plurality of apertures; and a second member defining a second plurality of apertures, the second member pivotably connected to the first member, wherein the second member is configured to engage with the first member to form a closed configuration or pivot relative to the first member between an open configuration and a closed configuration, wherein in the closed configuration the first plurality of apertures and the second plurality of apertures are aligned and form a plurality of sample compartments. The tissue sample compartmentalization module permits individual sample environments for sectioned tissue microscope slides.
Disclosed herein are compositions comprising a solid substrate that is bound to a multivalent cation-binding ligand. The composition can be used to associate positively-charged ion species with the solid substrate so as to facilitate separation methods in the solid phase. Also disclosed are methods of making and using the composition.
Described herein are compositions, methods, and kits for detecting diarrhea causing pathogens from porcine samples. One embodiment described herein is primer pairs and probes for multiplex polymerase chain reaction (PCR) based assays for the detection of diarrhea causing pathogens, such as Rotavirus A, Rotavirus B, and Rotavirus C. Other embodiments include methods and kits for detecting diarrhea causing pathogens.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
The present invention relates to a mixing apparatus for use in a fluid mixing equipment. In an embodiment, the mixing apparatus includes a hub having a top portion and a bottom portion and a central opening extending between the top and bottom portions along a centerline. A base flange extends outward from the bottom portion of the hub and one or more blades are coupled to the hub and extending to a shroud wall surrounding at least a portion of the top portion of the hub.
B01F 27/113 - Propeller-shaped stirrers for producing an axial flow, e.g. shaped like a ship or aircraft propeller
B01F 27/213 - Mixers with rotary stirring devices in fixed receptaclesKneaders characterised by their rotating shafts characterised by the connection with the drive
B01F 101/23 - Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
92.
COMPOSITIONS, KITS AND METHODS FOR DIRECT AMPLIFICATION FROM CRUDE BIOLOGICAL SAMPLES
Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the detection of target nucleic acids, including those from microbes and/or from infectious agents such as SARS-CoV-2 and other viruses. Embodiments described herein are designed to enable processing and analysis of the sample to detect target nucleic acids within the sample without requiring extraction and/or isolation of nucleic acid from the sample prior to subsequent processing steps. Samples analyzed can thus be “crude” biological samples that do not require pre-processing prior to placement in the workflow.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
93.
MEMBRANE-PENETRATING PEPTIDES TO ENHANCED TRANSFECTION AND COMPOSITIONS AND METHODS FOR USING SAME
The present invention is directed to non-naturally occurring peptides containing a membrane-penetrating amino acid sequence and further at least one polycationic moiety or peptide sequence. The peptides are suitable for use in delivery a cargo to the interior of a cell. Suitable cargo includes nucleic acid molecules (including DNA, RNA or PNA), polypeptides, or other biologically active molecules. The present invention is further directed to transfection complexes containing the non-naturally occurring peptides of the present invention in non-covalent association with at least one cationic lipid and a cargo to be delivered to the interior of a cell. The invention further relates to methods for the preparation and use of the non-naturally occurring peptides for the formation of transfection complexes and the delivery of a cargo to the interior of a cell in culture, an animal or a human. The invention also relates to compositions and kits useful for transfecting cells.
C12N 15/88 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using liposome vesicle
A61K 47/54 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
A61K 47/64 - Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
C07K 14/00 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof
Disclosed herein are methods of genotyping a crude nucleic acid sample, the methods including: subjecting the crude nucleic acid sample to a polymerase chain reaction (PCR) mixture directly after obtaining the crude nucleic acid sample from a source; incubating the crude nucleic acid sample in the PCR mixture for a set period of time; performing rapid PCR on the incubated crude nucleic acid sample; and analyzing results from the rapid PCR to determine the nucleic acid sample's genotype. Methods of detecting a disease in a subject are also disclosed.
The present invention is directed generally to systems and methods suitable for high level expression of recombinant proteins in suspension CHO cells. In particular, the invention allows introduction of the invention obviates the need to replace, replenish or supplement the growth medium during the procedure. The invention also relates to compositions and kits useful for culturing and transforming/transfecting suspension CHO cells.
Disclosed herein are methods of genotyping a crude nucleic acid sample, the methods including: subjecting the crude nucleic acid sample to a polymerase chain reaction (PCR) mixture directly after obtaining the crude nucleic acid sample from a source; performing rapid PCR on the crude nucleic acid sample by subjecting the crude nucleic acid sample to a first plurality of amplification cycles and followed by at least one second amplification cycle; and analyzing results from the rapid PCR to determine the nucleic acid sample's genotype. Each cycle of the first plurality of amplification cycles includes performing denaturation followed by annealing and extension without collecting amplification data. The at least second amplification cycle includes performing denaturation followed by annealing and extension while simultaneously collecting amplification data. Methods of detecting a disease in a subject are also disclosed.
INVITROGEN BIOSERVICES INDIA PRIVATE LIMITED (India)
Inventor
Rystrom, Larry
Ippolito, Kim
Rognin, Nicolas
K Y, Pradeep
Katiki, Naga Harshini
Hamilton, Mary Kristina
Kearns, Austin
Abstract
Systems and methods for identifying clumps (or groupings) of cells in cell counting systems. One example method executed by an electronic processing device for an image processing system includes receiving an image, captured by an imaging device, of a sample having a plurality of cells. The image includes labeling data for each of the plurality of cells. The method includes applying filters, determined based on the labeling data, to the image, processing the image to identify a plurality of groupings of the plurality of cells, and fitting an ellipse around each of the groupings by determining ellipse data for each of the ellipses. Each respective grouping of the plurality of groupings includes at least two cells having a same viability whose cell membranes are touching at least one other cell in the respective grouping.
INVITROGEN BIOSERVICES INDIA PRIVATE LIMITED (India)
Inventor
Rystrom, Larry
Ippolito, Kim
Rognin, Nicolas
K Y, Pradeep
Katiki, Naga Harshini
Hamilton, Mary Kristina
Kearns, Austin
Abstract
Systems and methods for identifying clumps (or groupings) of cells in cell counting systems. One example method executed by an electronic processing device for an image processing system includes receiving an image, captured by an imaging device, of a sample having a plurality of cells. The image includes labeling data for each of the plurality of cells. The method includes applying filters, determined based on the labeling data, to the image, processing the image to identify a plurality of groupings of the plurality of cells, and fitting an ellipse around each of the groupings by determining ellipse data for each of the ellipses. Each respective grouping of the plurality of groupings includes at least two cells having a same viability whose cell membranes are touching at least one other cell in the respective grouping.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Competent cells for gene transfer used in non-clinical molecular biology and microbiology research and accompanying multi-well array plates sold as a unit
