G01N 15/1404 - Manipulation du flux, p. ex. focalisation hydrodynamique
A61M 1/36 - Autre traitement du sang dans une dérivation du système circulatoire naturel, p. ex. adaptation de la température, irradiation
G01N 15/12 - Recherche de particules individuelles en mesurant des effets électriques ou magnétiques en observant des changements de résistance ou d’impédance à travers des fentes traversées par des particules individuelles, p. ex. en utilisant le principe de Coulter
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
G01N 15/1409 - Manipulation des échantillons, p. ex. injection des échantillons
G01N 21/17 - Systèmes dans lesquels la lumière incidente est modifiée suivant les propriétés du matériau examiné
G01N 29/22 - Recherche ou analyse des matériaux par l'emploi d'ondes ultrasonores, sonores ou infrasonoresVisualisation de l'intérieur d'objets par transmission d'ondes ultrasonores ou sonores à travers l'objet Détails
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
Methods and systems that use cloud-based resources and assay definition files for a local server system to control a sequencing device and process sequencing data resulting from a sequencing run for an assay are described. A method may include receiving, at a local server system, an assay definition file from a server of a cloud computing and storage system. The assay definition file may include code modules for configuring an assay. The code modules may be stored in a memory of the local server system. The server system may receive sequencing data from a sequencing device. The sequencing device may produce the sequencing data during a sequencing run performed for the assay. The server system may apply an analysis pipeline for the assay to the sequencing data. The analysis pipeline includes analysis steps executed in accordance with the code modules from the assay definition file to produce assay analysis results.
G16H 40/63 - TIC spécialement adaptées à la gestion ou à l’administration de ressources ou d’établissements de santéTIC spécialement adaptées à la gestion ou au fonctionnement d’équipement ou de dispositifs médicaux pour le fonctionnement d’équipement ou de dispositifs médicaux pour le fonctionnement local
G06F 21/62 - Protection de l’accès à des données via une plate-forme, p. ex. par clés ou règles de contrôle de l’accès
G16B 30/10 - Alignement de séquenceRecherche d’homologie
G16B 50/00 - TIC pour la programmation d’outils ou de systèmes de bases de données spécialement adaptées à la bio-informatique
G16H 40/40 - TIC spécialement adaptées à la gestion ou à l’administration de ressources ou d’établissements de santéTIC spécialement adaptées à la gestion ou au fonctionnement d’équipement ou de dispositifs médicaux pour la gestion d’équipement ou de dispositifs médicaux, p. ex. pour planifier la maintenance ou les mises à jour
G16H 40/67 - TIC spécialement adaptées à la gestion ou à l’administration de ressources ou d’établissements de santéTIC spécialement adaptées à la gestion ou au fonctionnement d’équipement ou de dispositifs médicaux pour le fonctionnement d’équipement ou de dispositifs médicaux pour le fonctionnement à distance
3.
COMPOSITION AND METHOD FOR DETECTION OF BIOMOLECULES VIA AROMATIC LABELING USING HETEROCYCLIC COMPOUNDS
Disclosed herein is a composition comprising a medium and a heterocyclic compound, for detecting a biomolecule in a sample. More specifically, the composition disclosed herein can be used to detect one or more biomolecules comprising one or more aromatic amino acid residues by producing an amino acid conjugate upon associating with the aromatic amino acid residue of the biomolecule. Also disclosed herein are methods of making and using the composition disclosed herein.
G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
Disclosed herein is a composition comprising a medium and a heterocyclic compound, for detecting a biomolecule in a sample. More specifically, the composition disclosed herein can be used to detect one or more biomolecules comprising one or more aromatic amino acid residues by producing an amino acid conjugate upon associating with the aromatic amino acid residue of the biomolecule. Also disclosed herein are methods of making and using the composition disclosed herein.
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
5.
COMPOSITIONS AND METHODS FOR IMMUNE REPERTOIRE SEQUENCING
The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire using genomic DNA from a biological sample. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Nucleic acid sequences of variable regions associated with the immune cell receptor are determined to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.
A method of identifying a copy number variations reads includes mapping reads to a reference genome, computing coverage for a plurality of tiles, and normalizing the coverage for a tile based on a coverage mode across the plurality of tiles. The method further includes determining a score for the plurality of tiles being in a plurality of ploidy states, determining a maximum score path across the tiles and through the ploidy states, and providing a copy number determination based on the maximum score path.
A method for nucleic acid sequencing may include disposing a plurality of template nucleic acid molecules in a plurality of defined spaces disposed on a sensor array, at least some of the plurality of template nucleic acid molecules having a sequencing primer and a polymerase operably bound therewith; advancing one or more nucleotide species over the plurality of template nucleic acid molecules with the sequencing primer and the polymerase operably bound therewith; measuring a signal generated by nucleotide incorporations resulting from advancing the one or more nucleotide species; and exposing the plurality of template nucleic acid molecules to a cleaving reagent subsequent to the advancing and measuring. The cleaving reagent can remove labeling reagents attached to the one or more nucleotide species. The advancing and measuring steps can be performed for different orders of the one or more nucleotide species prior to a subsequent exposing of the plurality of template nucleic acid molecules to the cleaving reagent.
Methods, systems, and apparatuses for navigating data visualizations within a user interface. One method includes providing particle data, which may be captured by an imaging device, to the user interface and receiving, from the user interface, a plurality of coordinates for a gate related to the particle data. The method also includes receiving, from the user interface, a configuration for a current view of the particle data, and, in response to determining the gate is off-plot for the current view based on the plurality of coordinates for the gate and the configuration for the current view, adding a selectable indicator to the current view of the particle data representing the gate. The method further includes, in response to receiving a selection of the indicator, modifying the current view of the particle data to display the gate.
Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C.
Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23° C.
Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.
Methods, systems, and apparatuses for navigating data visualizations within a user interface. One method includes providing particle data, which may be captured by an imagin device, to the user interface and receiving, from the user interface, a plurality of coordinates for a gate related to the particle data. The method also includes receiving, from the user interface, a configuration for a current view of the particle data, and, in response to determining the gate is off-plot for the current view based on the plurality of coordinates for the gate and the configuration for the current view, adding a selectable indicator to the current view of the particle data representing the gate. The method further includes, in response to receiving a selection of the indicator, modifying the current view of the particle data to display the gate.
Disclosed herein are machine learning-based particle classification systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a particle classification system may include: an electronic processing device configured to: receive, from an imaging flow cytometer instrument, a test set comprising unlabeled data to classify; pool the test set and a training set into a concatenated dataset comprising a plurality of parameters, wherein the training set comprises labeled data; normalize the concatenated dataset by bringing the variance to one for each of the plurality of parameters; non-linearly reduce a dimensionality of the normalized concatenated dataset to a reduced dimension space; compute classification parameters by classifying the unlabeled data from the reduced dimension space using the labeled data from the reduced dimension space; and provide the classification parameters for further processing.
G06V 20/69 - Objets microscopiques, p. ex. cellules biologiques ou pièces cellulaires
G06F 18/2413 - Techniques de classification relatives au modèle de classification, p. ex. approches paramétriques ou non paramétriques basées sur les distances des motifs d'entraînement ou de référence
G06V 10/762 - Dispositions pour la reconnaissance ou la compréhension d’images ou de vidéos utilisant la reconnaissance de formes ou l’apprentissage automatique utilisant le regroupement, p. ex. de visages similaires sur les réseaux sociaux
G06V 10/774 - Génération d'ensembles de motifs de formationTraitement des caractéristiques d’images ou de vidéos dans les espaces de caractéristiquesDispositions pour la reconnaissance ou la compréhension d’images ou de vidéos utilisant la reconnaissance de formes ou l’apprentissage automatique utilisant l’intégration et la réduction de données, p. ex. analyse en composantes principales [PCA] ou analyse en composantes indépendantes [ ICA] ou cartes auto-organisatrices [SOM]Séparation aveugle de source méthodes de Bootstrap, p. ex. "bagging” ou “boosting”
13.
METHODS AND SYSTEMS FOR VISUALIZING AND EVALUATING DATA
A computer-implemented method of generating a digital polymerase chain reaction (dPCR) result is provided. The method includes detecting a first set of emission data from a plurality of samples, each included in a sample region of a plurality of sample regions, at a first time during an amplification period. The method further includes determining a positive or negative amplification determination for each sample of the plurality of samples based in part on the first set of emission data. A dPCR result is generated based on the positive or negative amplification determinations for the plurality of samples.
G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
14.
SYSTEMS AND METHODS FOR PARTICLE CLASSIFICATION USING MACHINE LEARNING
Disclosed herein are machine learning-based particle classification systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, a particle classification system may include: an electronic processing device configured to: receive, from an imaging flow cytometer instrument, a test set comprising unlabeled data to classify; pool the test set and a training set into a concatenated dataset comprising a plurality of parameters, wherein the training set comprises labeled data; normalize the concatenated dataset by bringing the variance to one for each of the plurality of parameters; non-linearly reduce a dimensionality of the normalized concatenated dataset to a reduced dimension space; compute classification parameters by classifying the unlabeled data from the reduced dimension space using the labeled data from the reduced dimension space; and provide the classification parameters for further processing.
A method of loading beads on a sensor substrate includes applying a suspension including beads to a flow cell defined over a sensor substrate. The sensor substrate includes a plurality of wells. The beads at least partially deposit into the plurality of wells. The method also includes removing liquid from the flow cell, evaporating liquid from the flow cell, for example, by drawing air through the flow cell; and applying a hydrating solution to the flow cell.
C12Q 1/37 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une hydrolase faisant intervenir une peptidase ou une protéinase
G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
C07K 5/02 - Peptides ayant jusqu'à quatre amino-acides dans une séquence entièrement déterminéeLeurs dérivés contenant au moins une liaison peptidique anormale
17.
NUCLEOTIDE TRANSIENT BINDING FOR SEQUENCING METHODS
Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
C12Q 1/6804 - Analyse d’acides nucléiques utilisant des immunogènes
C12Q 1/6818 - Tests d’hybridation caractérisés par les moyens de détection impliquant l’interaction de plusieurs marqueurs, p. ex. transfert d’énergie de résonance
C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
A high data rate integrated circuit, such as an integrated circuit including a large sensor array, may be implemented using clock multipliers in individual power domains, coupled to sets of transmitters, including a transmitter pair configuration. Reference clock distribution circuitry on the integrated circuit distributes a relatively low speed reference clock. In a transmitter pair configuration, each pair comprises a first transmitter and a second transmitter in a transmitter power domain. Also, each pair of transmitters includes a clock multiplier connected to the reference clock distribution circuitry, and disposed between the first and second transmitters, which produces a local transmit clock.
Disclosed are compositions, kits, and methods for use in applications involving electrophoretic separation of nucleic acids, including capillary electrophoresis applications in which nucleic acid samples are labelled with dyes, size-separated, and subjected to short tandem repeat (STR) analysis. A sample containing DNA is mixed with a composition that includes at least one set of primers that target an STR region of the sample nucleic acid, a set of short quantification primers (QS primers) that target a first multi-copy sequence (QS sequence) of the sample nucleic acid, and a set of long quantification primers (QL primers) that target a second multi-copy sequence (QL sequence) of the sample nucleic acid, wherein the QS sequence is shorter than the QL sequence.
C12Q 1/6888 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes
20.
Electrophoresis & Electrotransfer Devices, Systems & Methods
Life Technologies Holdings PTE Limited (Singapour)
Inventeur(s)
Murakami, Marie
Bulloch, Kyle
Olson, Neil
Winnick, Ross
Teo, Wei Fuh
Thacker, Michael
Abrégé
The present disclosure provides systems for gel electrophoresis and electrotransfer comprising one or more chambers that can removably and interchangeably receive either an electrophoresis cassette, or an electrotransfer cassette, and provides an electrical interface for both electrophoresis and electrotransfer of biomolecules. The present disclosure also provides electrophoresis devices including clamps and electrotransfer cassettes and related devices. Methods for electrophoresis and electrotransfer using the systems and devices of the disclosure are also provided.
The present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. In particular, the disclosure relates to methods for introducing a nucleic acid cutting entity for DNA editing into cells that are difficult to transfect. Generally, the nucleic acid cutting entity is introduced into the cell without the use of viral vectors. The disclosure also relates to cloning DNA molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and/or binding one or more DNA binding agents (TAL effector domain or truncated guide RNA bound by Cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and/or it increasing the accessibility of the target locus to further enzymatic modifications. The methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.
Methods, systems and non-transitory machine-readable storage medium are provided to mitigate insertion errors and deletion errors in STR sequences and improve accuracy in determination of the number of repeats. A method includes determining one or more optimum clusters for a set of flow space signal measurements, wherein at least one of the optimum clusters is associated with a homopolymer length, modifying a base call at the position in the repeat region sequence to the homopolymer length associated with the optimum cluster to produce a corrected repeat region sequence, thereby correcting an insertion error or a deletion error. The method may further include detecting variations in the flanks associating those variations with the length of the STR.
G16B 5/00 - TIC spécialement adaptées à la modélisation ou aux simulations dans la biologie des systèmes, p. ex. réseaux de régulation génétique, réseaux d’interaction entre protéines ou réseaux métaboliques
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
G16B 40/00 - TIC spécialement adaptées aux biostatistiquesTIC spécialement adaptées à l’apprentissage automatique ou à l’exploration de données liées à la bio-informatique, p. ex. extraction de connaissances ou détection de motifs
The present set of embodiments relate to a bioproduction system, method, and apparatus for creating a scalable bioreactor system. Specifically, the present set of embodiments enable the determination of bioreaction performance characteristics of a commercial scale by matching operational parameters between a small test scale bioreaction to that of a commercial scale bioreaction. The system and methods do not rely on simply making bioreactor apparatuses across scales the same dimensionally which would not account for differences in fluid dynamic properties between very small to very large volumes, but requires tuning of a variety of systems (mixing assembly, sparger system, and headspace airflow system) in conjunction with one another to achieve predictive outcomes.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
B01F 23/233 - Mélange de gaz avec des liquides en introduisant des gaz dans des milieux liquides, p. ex. pour produire des liquides aérés en utilisant des agitateurs entraînés munis d’éléments d'agitation complètement immergés
B01F 27/054 - Agitateurs déformables, p. ex. déformés par une force centrifuge appliquée en cours de fonctionnement
B01F 27/114 - Agitateurs de forme hélicoïdale, c.-à-d. agitateurs comprenant une bande de forme hélicoïdale ou des sections de bande de forme hélicoïdale
B01F 27/213 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins caractérisés par leurs arbres de rotation caractérisés par la liaison avec l'entraînement
B01F 27/90 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins avec des agitateurs tournant autour d'un axe sensiblement vertical avec des palettes ou des bras
B01F 27/92 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins avec des agitateurs tournant autour d'un axe sensiblement vertical avec des hélices ou des vis
B01F 35/41 - Montage ou support des arbres d'agitation ou des unités d'agitation sur les récipients
B01F 35/43 - Récipients de soutien sur des cadres ou des supports
B01F 35/513 - Récipients souples, p. ex. sacs supportés par des conteneurs rigides
B01F 101/44 - Mélange d'ingrédients pour la microbiologie, l'enzymologie, la culture in vitro ou la manipulation génétique
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/06 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz avec agitateur, p. ex. avec agitateur à turbine
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
C12N 5/071 - Cellules ou tissus de vertébrés, p. ex. cellules humaines ou tissus humains
24.
METHODS FOR DETECTING MUTATION LOAD FROM A TUMOR SAMPLE
A targeted panel with low sample input requirements from a tumor only sample may be processed to estimate mutation load in a tumor sample. The method may include detecting variants in nucleic acid sequence reads corresponding to targeted locations in the tumor sample genome; annotating detected variants with an annotation information from a population database; filtering the detected variants, wherein the filtering rule set retains the somatic variants and removes germ-line variants; counting the identified somatic variants to give a number of somatic variants; determining a number of bases in covered regions of the targeted locations in the tumor sample genome; and calculating a number of somatic variants per megabase, provides an estimate of the mutation load per megabase in the tumor sample genome.
A lentiviral vector production system comprises (a) a lentiviral culture supplement to control cell growth, (b) a transfection reagent comprising DHDMS, DOPE, and cholesterol to increase transfection efficiency, (c) a lentiviral production enhancer comprising sodium propionate, sodium butyrate, and caffeine to boost lentiviral production, wherein the lentiviral vector production system is serum-free. A method of lentiviral vector production comprises using the lentiviral production system. Another method for lentiviral vector production comprises (a) culturing eukaryotic cells in a serum-free medium, (b) providing a lentiviral culture supplement to control cell growth, (c) transfecting the cells with a lentiviral vector using a transfection reagent comprising DHDMS, DOPE, and cholesterol to increase transfection efficiency, and (d) providing a lentiviral production using a lentiviral production enhancer comprising sodium propionate, sodium butyrate capable of boosting lentiviral production.
THERMO SCIENTIFIC PORTABLE ANALYTICAL INSTRUMENTS INC. (USA)
LIFE TECHNOLOGIES CORPORATION (USA)
Inventeur(s)
Doherty, Walter
Lee, Lisa
Fisher, Brandon
Benson, Katelyn
Abrégé
A modular accessory for orienting a light path at different angles to an optical axis of a spectrometer is described. The modular accessory includes an attachment module configured to couple to a Raman spectrometer. The attachment module attaches to a base module which includes a visible light imager. An objective module couples to the base module and includes an objective configured to provide an optical path for the sample light beam travelling from a sample along a light path to an objective lens of the objective, through the objective, and to the input for the sample light beam of the base module. The modules can provide tilt and swivel angles to orient the light path at different angles. Portable Raman systems including the modular accessory and a Raman spectrometer are also described as well as methods for using the systems.
Systems and methods for a tubing disconnect assembly are disclosed. In an embodiment the system includes a first bioprocessing unit, a second bioprocessing unit, a flexible tubing in fluid communication with the first and second bioprocessing unit, and a tubular collar having a middle portion sandwiched between a first and second swaged end portions, and secured around at least a portion of the tubing, the collar configured to be disconnected by a disconnecting device in the middle region extending between the first and second swaged end portions.
A method includes exposing template polynucleotide strands, sequencing primers, and polymerase to flows of nucleotide species; obtaining a series of measured intensity values and randomly selecting a training subset therefrom; generating series of base calls using a base caller and aligning the series of base calls to a reference genome using an aligner; determining intensity value thresholds and parameters of a linear transformation corresponding to different homopolymer lengths and nucleotide species; generating series of base calls corresponding to the series of measured intensity values using at least some of the parameters of a linear transformation; and recalibrating the series of base calls corresponding to the plurality of series of measured intensity values using at least some of the intensity value thresholds.
A method of mixing a fluid includes at least partially unfolding a collapsible bag bounding a compartment, the collapsible bag containing in the compartment at least a portion of an elongated drive line or drive shaft and an impeller secured to the drive line or drive shaft, the impeller including a plurality of impeller blades that are pivotable relative to the drive line or drive shaft, at least one of the plurality of impeller blades being in a collapsed position. A fluid is delivered into the compartment of the collapsible bag. The drive line or drive shaft is then rotated so as to rotate the impeller within the compartment and mix the fluid therein, the at least one of the plurality of impeller blades pivoting from the collapsed position to an expanded position as the impeller is rotated within the compartment.
B01F 27/21 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins caractérisés par leurs arbres de rotation
B01F 27/054 - Agitateurs déformables, p. ex. déformés par une force centrifuge appliquée en cours de fonctionnement
B01F 27/07 - Agitateurs caractérisés par leur montage sur l’arbre
B01F 27/072 - Agitateurs caractérisés par leur montage sur l’arbre caractérisés par la disposition des agitateurs par rapport à l'axe de rotation
B01F 27/1125 - Agitateurs caractérisés par la configuration des agitateurs avec des bras, des pales ou des lames avec des pales ou des lames s'étendant parallèlement ou obliquement par rapport à l'axe de l'agitateur
B01F 27/191 - Agitateurs avec plusieurs éléments de mélange montés en séquence sur un même axe avec des éléments similaires
B01F 27/88 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins avec des agitateurs tournant autour d'un axe sensiblement vertical avec une unité récipient-agitateur séparée qui est adaptée pour être couplée à un mécanisme d'entraînement
B01F 27/906 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins avec des agitateurs tournant autour d'un axe sensiblement vertical avec des palettes ou des bras avec un axe fixe
B01F 35/41 - Montage ou support des arbres d'agitation ou des unités d'agitation sur les récipients
B01F 35/513 - Récipients souples, p. ex. sacs supportés par des conteneurs rigides
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/06 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz avec agitateur, p. ex. avec agitateur à turbine
30.
Magnetic Particle Separation System with Flexible Bioprocessing Container
A magnetic particle separation system includes a magnetic field generating device having an upper surface with a receiving area formed thereon; and a magnetic field generating element disposed beneath the upper surface, the magnetic field generating element being configured to produce a magnetic field above the upper surface. A container assembly is disposed on the upper surface and includes: a flexible container having an outer wall with an interior surface that at least partially bounds an internal compartment, the outer wall having a front side and an opposing back side with the internal compartment disposed therebetween; a fluid inlet extending through the outer wall at the front side; a fluid outlet extending through the outer wall at the front side; and a first partition projecting into the internal compartment from the front side between the fluid inlet and the fluid outlet.
B03C 1/30 - Combinaisons avec d'autres dispositifs, non prévues ailleurs
C07K 16/28 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains contre des récepteurs, des antigènes de surface cellulaire ou des déterminants de surface cellulaire
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
The disclosure generally relates to systems, methods, and apparatuses for magnetic bead loading. An example embodiment of the disclosure relates to an apparatus including a vertically oriented plate having a first major surface and a second major surface opposite the first major surface; a magnet holder securing a magnet in proximity to the first major surface of the vertically oriented plate; a drive mechanism coupled to the magnet holder and operable to move the magnet holder and magnet in parallel to the first major surface of the vertically oriented plate; and a substrate holder to receive a substrate and hold the substrate in a vertical orientation against the surface of the vertically oriented plate.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
The present disclosure provides freeze-dried eukaryotic cell culture media, feeds, and supplement formulations that have enhanced stability when stored refrigerated or at room temperature. The present disclosure contemplates freeze-dried eukaryotic cell culture media and supplement compositions having reduced moisture content and enhanced stability when compared to non-freeze-dried eukaryotic cell culture media, feed, and supplement compositions. Additional aspects include methods of freeze-drying eukaryotic cell culture media and supplement compositions to enhance stability. Further encompassed are methods for stabilizing eukaryotic cell culture media, feed, or supplement compositions by freeze-drying. Still further aspects include freeze-dried eukaryotic cell culture media, feed, and supplement compositions that are agglomerated or powered media compositions.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
A01N 1/162 - Procédés liés à la température, p. ex. suivant une courbe de température prédéfinie dans le temps
33.
METHODS, SYSTEMS, COMPUTER READABLE MEDIA, AND KITS FOR SAMPLE IDENTIFICATION
A method for sequencing a polynucleotide sample having a barcode sequence includes: introducing a series of nucleotides to the polynucleotide sample according to a predetermined order of nucleotide flows; obtaining a series of signals resulting from the introducing of nucleotides to the polynucleotide sample; and resolving the series of signals over the barcode sequence to render a flowspace string, wherein the flowspace string is a codeword of an error-correcting code that is (i) designed based on and adapted for use with the predetermined order of nucleotide flows, and (ii) capable of distinguishing any codeword in the error-correcting code from the other codewords in the error-correcting code in the presence of zero, one, and two errors.
C40B 20/04 - Identification des éléments d'une bibliothèque au moyen d'une étiquette, d'un marqueur ou d'un autre identificateur lisible ou détectable, p. ex. procédés de décodage
C40B 50/16 - Synthèse en phase solide, c.-à-d. dans laquelle au moins un bloc servant à créer la bibliothèque est lié à un support solide au cours de la création de la bibliothèqueProcédés particuliers de clivage à partir du support solide comprenant des étapes de codage
C40B 70/00 - Étiquettes ["tags"] ou marqueurs ["labels"] spécialement adaptés à la chimie combinatoire ou aux chimiothèques, p. ex. "tags" fluorescents ou codes-barres
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
Methods for determining an arm aneuploidy score in a tumor sample genome include selectively amplifying nucleic acid sequences at specific locations in the tumor genome using a targeted panel to generate sequence reads. Next, divide the genome locations into segments with homogeneous copy numbers based on log odds of heterozygous SNPs and CNV log ratios of the sequence reads. Identify gain and loss segments relative to a reference copy number and intersecting respective chromosome arms. Compare the cellularities of these segments to a minimum threshold. Retain the longest segment for the arm that meets the minimum cellularity and sum its total bases. Divide this total by the number of bases in the arm to yield a fraction. If this fraction meets a minimum threshold, filter the segment based on fold changes and determine gains or losses. Count the arms with called gains or losses to generate the arm aneuploidy score.
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
G16B 20/10 - Ploïdie ou détection du nombre de copies
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12Q 1/6888 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes
G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
An improved amebocyte lysate-based method for the detection of endotoxin is described. The method requires only a single incubation step and permits the use of various sample input volumes, allowing for greater flexibility while maintaining sensitive detection of endotoxin at concentrations ranging from 0.01 to 10 endotoxin units (EU)/mL. Kits for performing the endotoxin detection method are also described.
37.
SCALABLE SYSTEMS AND METHODS FOR CONTINUOUS TRANSFECTION OF CELLS
Methods and systems for improving the scalability and associated efficacy of continuous transfection processes, such as continuous transient transfection processes. The continuous transfection system includes at least a first input line and a second input line, wherein the first input line and the second input line are configured for sterile, fluid connection to a first reagent housing and a second reagent housing, respectively, a mixing chamber that is fluidly connected to the at least first and second input lines, a delivery line fluidly connected to the mixing chamber, wherein the delivery line is configured for sterile, fluid connection to a cell culture vessel, and a flow rate control component, such as a pump operable to control flow rate of liquid into the at least first and second input lines, throughout the mixing chamber, and into the cell culture vessel.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/06 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz avec agitateur, p. ex. avec agitateur à turbine
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
A method of biological sample analysis includes subjecting a biological sample comprising nucleic acid to an amplification reaction conducted in the presence of a detector probe and a calibrator probe, wherein the detector probe is configured to specifically interact with a first region of a wild type target nucleic acid sequence and emit a first emission signal in response to the interaction, and wherein the calibrator probe is configured to specifically interact with a second region of the wild type target nucleic acid sequence and emit a second emission signal in response to the interaction, the first region and the second region differing from each other. The method further includes calculating a ratio of the change in the first emission signal to the change of the second emission signal at a point in time of the amplification reaction.
Provided herein is a method of sequentially isolating DNA and RNA from a sample, the method comprising: contacting the sample with a solid support comprising surface silanol groups in the presence of an aqueous nucleic acid purification buffer to provide a DNA-bound solid support; removing the DNA-bound solid support from the sample; contacting the sample with a solid support comprising surface carboxyl groups in the presence of the aqueous nucleic acid purification buffer to provide an RNA-bound solid support; and removing the RNA-bound solid support from the fluid sample, wherein the aqueous nucleic acid purification buffer comprises a polar aprotic solvent. Also provided is a kit comprising said purification buffer and solid supports, the use of said kit in an automated nucleic acid analysis platform, and a nucleic acid analysis apparatus, comprising an automated nucleic acid analysis platform; and the aforementioned kit.
Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
G01N 27/30 - Électrodes, p. ex. électrodes pour testsDemi-cellules
Provided are methods and compositions for preparing a library of target nucleic acid sequences that are useful for assessing gene mutations for oncology biomarker profiling of samples. In particular, a target-specific primer panel is provided that allows for selective amplification of oncology biomarker target sequences in a sample. In one aspect, the invention relates to target-specific primers useful for selective amplification of one or more target sequences associated with oncology biomarkers from two or more sample types. In some aspects, amplified target sequences obtained using the disclosed methods, and compositions can be used in various processes including nucleic acid sequencing and used to detect the presence of genetic variants of one or more targeted sequences associated with oncology.
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
45.
MEMBRANE-PENETRATING PEPTIDES TO ENHANCED TRANSFECTION AND COMPOSITIONS AND METHODS FOR USING SAME
The present invention is directed to non-naturally occurring peptides containing a membrane-penetrating amino acid sequence and further at least one polycationic moiety or peptide sequence. The peptides are suitable for use in delivery a cargo to the interior of a cell. Suitable cargo includes nucleic acid molecules (including DNA, RNA or PNA), polypeptides, or other biologically active molecules. The present invention is further directed to transfection complexes containing the non-naturally occurring peptides of the present invention in non-covalent association with at least one cationic lipid and a cargo to be delivered to the interior of a cell. The invention further relates to methods for the preparation and use of the non-naturally occurring peptides for the formation of transfection complexes and the delivery of a cargo to the interior of a cell in culture, an animal or a human. The invention also relates to compositions and kits useful for transfecting cells.
C12N 15/88 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p. ex. co-transformation utilisant la micro-encapsulation, p. ex. utilisant des vésicules liposomiques
A61K 47/54 - Préparations médicinales caractérisées par les ingrédients non actifs utilisés, p. ex. les supports ou les additifs inertesAgents de ciblage ou de modification chimiquement liés à l’ingrédient actif l’ingrédient non actif étant chimiquement lié à l’ingrédient actif, p. ex. conjugués polymère-médicament l’ingrédient non actif étant un agent de modification l’agent de modification étant un composé organique
A61K 47/64 - Conjugués médicament-peptide, médicament-protéine ou médicament-acide polyaminé, c.-à-d. l’agent de modification étant un peptide, une protéine ou un acide polyaminé lié par covalence ou complexé à un agent thérapeutiquement actif
C07K 14/00 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés
46.
Dibenzoxanthene Quenchers, Uses, and Methods of Preparation
The present disclosure relates to dibenzoxanthene compounds that are efficient quenchers of fluorescence, for example in the far red and near infrared spectrum. Applications using the dibenzoxanthene quenching compounds and methods of making same are also described. Also disclosed is a method of detecting or quantifying a target nucleic acid molecule in a sample by polymerase chain reaction (PCR). The compounds have the following formula (I).
The present disclosure relates to dibenzoxanthene compounds that are efficient quenchers of fluorescence, for example in the far red and near infrared spectrum. Applications using the dibenzoxanthene quenching compounds and methods of making same are also described. Also disclosed is a method of detecting or quantifying a target nucleic acid molecule in a sample by polymerase chain reaction (PCR). The compounds have the following formula (I).
09 - Appareils et instruments scientifiques et électriques
Produits et services
Downloadable computer software used for analysis of scientific data and information for use in analyzing cells and particles using reflective and fluorescent properties produced by a flow cytometer apparatus or device
Devices, systems, and methods for measuring an analyte are disclosed. A device, system, or method can include a radiofrequency sensor comprising a radiofrequency transmitter and a radiofrequency receiver. A device, system, or method can be configured to transmit a radiofrequency signal from the transmitter to the receiver through a culture medium comprising the analyte. In some embodiments, a device, system, or method can include a controller configured to determine a concentration of the analyte based on a resultant signal produced at the receiver. A device, system, or method can comprise a radiofrequency sensor introducer. An introducer of a device, system, or method can comprise a first sensor sheath configured to receive a radiofrequency transmitter and a second sensor sheath configured to receive a radiofrequency receiver. In some implementations, an introducer of a device, system, or method can be configured to maintain a separation between the transmitter and receiver.
G01N 22/00 - Recherche ou analyse des matériaux par l'utilisation de micro-ondes ou d'ondes radio, c.-à-d. d'ondes électromagnétiques d'une longueur d'onde d'un millimètre ou plus
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
50.
METHODS FOR PARTNER AGNOSTIC GENE FUSION DETECTION
A method for detecting a gene fusion includes amplifying a nucleic acid sample in the presence of primer pool to produce a plurality of amplicons. The primer pool includes primers targeting a plurality of exon-exon junctions of a driver gene. The amplicons correspond to the exon-exon junctions. The amplicons are sequenced and aligned to a reference sequence. The number of reads corresponding to each amplicon is normalized to give a normalized read count. A baseline correction is applied to the normalized read counts for the amplicons to form corrected read counts. A binary segmentation score is calculated for each corrected read count. A predicted breakpoint for the gene fusion is determined based on the amplicon index corresponding to the maximum absolute binary segmentation score. Gene fusion events may be detected in a partner agnostic manner, i.e. without prior knowledge of the specific fusion partner genes or specific breakpoint information.
The present disclosure relates to new and useful azaindole cyanine (pyrrolo pyridine cyanine) compounds. Use of the compounds as dyes that operate in the far-red and near-IR spectral region in biological imaging, and methods of making the compounds are also disclosed herein. The azaindole cyanine (pyrrolo pyridine cyanine) compounds have formulas (I) and (II).
The present disclosure relates to new and useful azaindole cyanine (pyrrolo pyridine cyanine) compounds. Use of the compounds as dyes that operate in the far-red and near-IR spectral region in biological imaging, and methods of making the compounds are also disclosed herein. The azaindole cyanine (pyrrolo pyridine cyanine) compounds have formulas (I) and (II).
C09B 23/08 - Colorants méthiniques ou polyméthiniques, p. ex. du type cyanine caractérisés par la chaîne méthinique contenant un nombre impair de groupes CH plus de trois groupes CH, p. ex. polycarbocyanines
G01N 33/533 - Production de composés immunochimiques marqués avec un marqueur fluorescent
The present set of embodiments relate to a system, method, and apparatus for forming a sanitary connection between two pipes or tubes. The apparatus may include two clamp members capable of closing around the ends of two adjoining pipes. Attachment regions may serve as a connection or pivot point and closure regions may lock the two clamp members in place. Systems and methods to prevent damage to the pipes or tubes are disclosed as well as systems and methods to bring the clamp members from an open configuration to a closed and secure configuration.
F16L 23/08 - Raccords à brides les brides étant raccordées par des organes tendus dans le plan radial raccordées par broche et écrou fileté disposés tangentiellement
F16L 23/04 - Raccords à brides les brides étant raccordées par des organes tendus dans le plan radial
F16L 23/06 - Raccords à brides les brides étant raccordées par des organes tendus dans le plan radial raccordées par leviers à mouvement de rotule
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
Produits et services
Kits consisting primarily of antibody derivatives and
reagents relating to the use and preparation of labeled
antibody conjugates for use in life sciences and biomedical
research, namely, for use in scientific or research use, but
excluding water quality, water filtration, and water
treatment.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
Produits et services
Biochemical reagents for use in scientific, environmental,
food, and forensic research; reagent kits comprised of
biochemical reagents for use in scientific, environmental,
food and forensic research; biochemical reagents for use in
a scientific apparatus for chemical or biological analysis;
reagent kits comprised of biochemical reagents for use in a
scientific apparatus for chemical or biological analysis.
Disclosed herein are aspects of a composition, typically comprising a functionalized magnetic particle with an outer surface comprising a ligand on the outer surface, wherein the ligand is bound to a permeabilized cell comprising a nucleic acid for generating a nucleic acid library. Also disclosed are a method of tagging a nucleic acid within at least one cell, a method of generating a nucleic acid library, systems configured for processing disclosed compositions and implementing disclosed methods, and kits comprising a composition or compositions for use with disclosed method aspects.
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
Produits et services
Kits consisting primarily of reagents and laboratory equipment, namely, microarrays sold as a unit for scientific research use Laboratory equipment, namely, microarrays
66.
SYSTEMS AND METHODS FOR DETECTING HOMOPOLYMER INSERTIONS/DELETIONS
Systems and method for determining variants can receive mapped reads and determine a distribution of matched-filter residuals distribution from a plurality of reads at a homopolymer region. The distribution of matched-filter residuals can be fit to uni-modal and bi-modal models. Based on the model that best fits the distribution of matched-filter residuals, the heterozygosity of the sample and the absence or presence of an insertion/deletion in the homopolymer can be determined.
Cell analysis systems and instruments provide an end user with time-course graphic and imaging data of individual cellular responses as elicited by perturbations in the cellular microenvironment and monitored by ChemFET sensors. Imaging data of cellular responses can be presented as an electroscopic image, which is an image taken at a defined sampling interval of cellular response from sensors covering a cell footprint or an image of cellular response for sensors detecting cellular effluent. Electroscopic imaging can be compared to optical imaging to provide an additional dimension of information regarding cell analysis. For example, comparing optical imaging of immunocytochemical studies using known marker panels provides a functional and phenotypic signature of data obtained for each cell analyzed using a ChemFET-based cell analysis system.
An artificial neural network is applied to a plurality of flow predictor features to generate a flow space probability of error for a base call. A base quality value for the base call is determined based on the flow space probability of error. The base call and flow predictor features are based on the flow space signal measurements generated in response to the nucleotide flow to the reaction confinement region. For an array of reaction confinement regions, a plurality of parallel neural networks is applied to produce a probability of error for each reaction confinement region. A given neural network of the parallel neural networks is applied to the plurality of flow predictor features corresponding to a given reaction confinement region in the array to provide the flow space probability of error for the given reaction confinement region.
A method for nucleic acid sequencing includes: receiving a signal comprising measurements of a parameter measured in response to a plurality of nucleotide flows flowed in a space comprising a sample nucleic acid; normalizing the signal to obtain a normalized signal; adaptively normalizing the normalized signal to obtain an adaptively normalized signal; and predicting a sequence of base calls corresponding to the sample nucleic acid using the adaptively normalized signal.
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
G01N 27/27 - Association de plusieurs systèmes ou cellules de mesure, chacun mesurant un paramètre différent, dans laquelle les résultats des mesures peuvent être, soit utilisès indépendamment, les systèmes ou les cellules étant physiquement associés, soit combinés pour produire une valeur représentative d'un autre paramètre
G01N 27/414 - Transistors à effet de champ sensibles aux ions ou chimiques, c.-à-d. ISFETS ou CHEMFETS
G16B 25/00 - TIC spécialement adaptées à l’hybridationTIC spécialement adaptées à l’expression de gènes ou de protéines
G16B 30/10 - Alignement de séquenceRecherche d’homologie
Sample preparation methods for in situ RNA or DNA analysis, methods and compositions used in such methods are provided. Methods provided herein allow DNA or RNA preparation and downstream analysis to be carried out in the same tube or on an aliquot of the prepared sample without centrifugation or further purification. The compositions and methods provided herein can advantageously be used on a variety of samples, including cultures of cell lines and/or primary cells. The preparation process is amenable to high throughput processing using manual or robotic platforms.
A method for user guided initiating of an instrument includes receiving a run plan via a user interface of the instrument; indicating on the user interface, based on the run plan, a consumable to be provided to the instrument; detecting the presence of the consumable using a vision system; and indicating the presence of the consumable via the user interface.
G06T 1/00 - Traitement de données d'image, d'application générale
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
73.
LIPIDS FOR DELIVERY OF NUCLEIC ACIDS TO EUKARYOTIC CELLS
Ionizable lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
C07C 215/28 - Composés contenant des groupes amino et hydroxy liés au même squelette carboné ayant des groupes hydroxy et des groupes amino liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant non saturé et contenant des cycles aromatiques à six chaînons
C07C 217/42 - Composés contenant des groupes amino et hydroxy éthérifiés liés au même squelette carboné ayant des groupes hydroxy éthérifiés et des groupes amino liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant acyclique et saturé ayant des groupes hydroxy éthérifiés et au moins deux groupes amino liés au squelette carboné
C07C 219/06 - Composés contenant des groupes amino et hydroxy estérifiés liés au même squelette carboné ayant des groupes hydroxy estérifiés et des groupes amino liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant acyclique et saturé les groupes hydroxy étant estérifiés par des acides carboxyliques ayant les groupes carboxyle estérifiants liés à des atomes d'hydrogène ou à des atomes de carbone acycliques d'un squelette carboné acyclique saturé
C07C 219/08 - Composés contenant des groupes amino et hydroxy estérifiés liés au même squelette carboné ayant des groupes hydroxy estérifiés et des groupes amino liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant acyclique et saturé au moins un des groupes hydroxy étant estérifié par un acide carboxylique ayant le groupe carboxyle estérifiant lié à un atome de carbone acyclique d'un squelette carboné acyclique non saturé
C07C 229/12 - Composés contenant des groupes amino et carboxyle liés au même squelette carboné ayant des groupes amino et carboxyle liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant acyclique et saturé ayant un seul groupe amino et un seul groupe carboxyle liés au squelette carboné l'atome d'azote du groupe amino étant lié de plus à des atomes de carbone acycliques ou à des atomes de carbone de cycles autres que des cycles aromatiques à six chaînons à des atomes de carbone de squelettes carbonés acycliques
C07C 229/22 - Composés contenant des groupes amino et carboxyle liés au même squelette carboné ayant des groupes amino et carboxyle liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant acyclique et saturé le squelette carboné étant substitué de plus par des atomes d'oxygène
C07C 229/26 - Composés contenant des groupes amino et carboxyle liés au même squelette carboné ayant des groupes amino et carboxyle liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant acyclique et saturé ayant plus d'un groupe amino lié au squelette carboné, p. ex. lysine
C07C 229/36 - Composés contenant des groupes amino et carboxyle liés au même squelette carboné ayant des groupes amino et carboxyle liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné contenant des cycles aromatiques à six chaînons avec au moins un groupe amino et un groupe carboxyle liés au même atome de carbone du squelette carboné
C07C 323/25 - Thiols, sulfures, hydropolysulfures ou polysulfures substitués par des halogènes, des atomes d'oxygène ou d'azote ou par des atomes de soufre ne faisant pas partie de groupes thio contenant des groupes thio et des atomes d'azote, ne faisant pas partie de groupes nitro ou nitroso, liés au même squelette carboné ayant les atomes de soufre des groupes thio liés à des atomes de carbone acycliques du squelette carboné le squelette carboné étant acyclique et saturé
C07D 209/20 - Radicaux substitués par des atomes de carbone comportant trois liaisons à des hétéro-atomes, avec au plus une liaison à un halogène, p. ex. radicaux ester ou nitrile substitués en outre par des atomes d'azote, p. ex. tryptophane
C07D 233/61 - Composés hétérocycliques contenant des cycles diazole-1, 3 ou diazole-1, 3 hydrogéné, non condensés avec d'autres cycles comportant deux liaisons doubles entre chaînons cycliques ou entre chaînons cycliques et chaînons non cycliques avec uniquement des atomes d'hydrogène ou des radicaux ne contenant que des atomes d'hydrogène et de carbone, liés aux atomes de carbone du cycle avec des radicaux hydrocarbonés, substitués par des atomes d'azote ne faisant pas partie d'un radical nitro, liés aux atomes d'azote du cycle
C07C 215/14 - Composés contenant des groupes amino et hydroxy liés au même squelette carboné ayant des groupes hydroxy et des groupes amino liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant saturé et acyclique l'atome d'azote du groupe amino étant de plus lié à des groupes hydrocarbonés substitués par des groupes amino
A61K 9/127 - Vecteurs à bicouches synthétiques, p. ex. liposomes ou liposomes comportant du cholestérol en tant qu’unique agent tensioactif non phosphatidylique
C07C 279/14 - Dérivés de la guanidine, c.-à-d. composés contenant le groupe les atomes d'azote liés par des liaisons simples ne faisant pas partie de groupes nitro ou nitroso ayant des atomes d'azote de groupes guanidine liés à des atomes de carbone acycliques d'un squelette carboné étant substitué de plus par des groupes carboxyle
Ionizable lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
A61K 31/164 - Amides, p. ex. acides hydroxamiques d'un acide carboxylique avec un aminoalcool, p. ex. céramides
A61K 48/00 - Préparations médicinales contenant du matériel génétique qui est introduit dans des cellules du corps vivant pour traiter des maladies génétiquesThérapie génique
C07C 217/42 - Composés contenant des groupes amino et hydroxy éthérifiés liés au même squelette carboné ayant des groupes hydroxy éthérifiés et des groupes amino liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant acyclique et saturé ayant des groupes hydroxy éthérifiés et au moins deux groupes amino liés au squelette carboné
C07C 229/04 - Composés contenant des groupes amino et carboxyle liés au même squelette carboné ayant des groupes amino et carboxyle liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant acyclique et saturé
C07C 229/28 - Composés contenant des groupes amino et carboxyle liés au même squelette carboné ayant des groupes amino et carboxyle liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant saturé et contenant des cycles
A61P 37/00 - Médicaments pour le traitement des troubles immunologiques ou allergiques
The present invention provides mutant DNA polymerases, polynucleotides encoding the polymerases, cassettes and vectors including such polynucleotides, and cells containing the polymerases, polynucleotides, cassettes, and/or vectors of the invention. The present invention also provides methods for synthesizing polynucleotides and kits including a DNA polymerase of the invention.
A detection system that operates with reduced sample waste and dead volume, the system including: a module configured to introduce a sample spacer into a sample; at least one light source, wherein the light source illuminates the sample spacer and the sample, wherein illumination of the sample spacer produces scattered light; and a detection device configured to initiate acquisition of data related to the sample in response to scattered light detected by the detection device.
Examples described herein provide systems and methods for quantifying cells. An example method includes receiving at least one image, improving a contrast of the at least one image to generate a contrast image, and performing a fit operation on the contrast image to generate a processed image. The method includes applying a filter to the processed image to generate a filtered image, identifying cells within the filtered image, and providing an output image including an indication of the cells.
A detection system that operates with reduced sample waste and dead volume, the system including: a module configured to introduce a sample spacer into a sample; at least one light source, wherein the light source illuminates the sample spacer and the sample, wherein illumination of the sample spacer produces scattered light; and a detection device configured to initiate acquisition of data related to the sample in response to scattered light detected by the detection device.
A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.
Examples described herein provide systems and methods for quantifying cells. An example method includes receiving at least one image, improving a contrast of the at least one image to generate a contrast image, and performing a fit operation on the contrast image to generate a processed image. The method includes applying a filter to the processed image to generate a filtered image, identifying cells within the filtered image, and providing an output image including an indication of the cells.
G06T 7/136 - DécoupageDétection de bords impliquant un seuillage
G06V 10/50 - Extraction de caractéristiques d’images ou de vidéos en effectuant des opérations dans des blocs d’imagesExtraction de caractéristiques d’images ou de vidéos en utilisant des histogrammes, p. ex. l’histogramme de gradient orienté [HoG]Extraction de caractéristiques d’images ou de vidéos en utilisant l’addition des valeurs d’intensité d’imageAnalyse de projection
G06V 20/69 - Objets microscopiques, p. ex. cellules biologiques ou pièces cellulaires
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
Produits et services
(1) Biochemical reagents for use in scientific, environmental, food, and forensic research; reagent kits comprised of biochemical reagents for use in scientific, environmental, food and forensic research; biochemical reagents for use in a scientific apparatus for chemical or biological analysis; reagent kits comprised of biochemical reagents for use in a scientific apparatus for chemical or biological analysis.
Systems and method for identifying gene fusions can obtain sequencing information for a plurality of amplicons from a nucleic acid sample. The sequencing information can include a plurality of reads that are initially partially mapped to a reference sequence. Fragments may be generated by splitting the partially mapped reads into mapped and unmapped fragments, and the fragments may be remapped to the reference sequence. Gene fusions can be identified based on reads where the first fragment maps to a first gene and the second fragment maps to a second gene.
Campylobacter spSalmonella spShigellasp./Enteroinvasive Escherichia coli (EIEC)Escherichia coli Escherichia coliEscherichia coli stx2/Shiga toxin B. Other embodiments include methods and kits for detecting diarrhea causing pathogens.
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
A61K 39/40 - AnticorpsImmunoglobulinesImmunsérum, p. ex. sérum antilymphocitaire bactériens
90.
METHODS FOR GAS FILTRATION IN FLUID PROCESSING SYSTEMS
A method for filtering a gas comprises passing a gas through a compartment of a filter assembly, the filter assembly comprising: an inlet opening; a first outlet opening; a casing comprising polymeric film and bounding the compartment, the compartment communicating with the inlet opening and the first outlet opening; and a first filter at least partially disposed within the compartment. The method further comprising forming a first seal across a first section of the casing at a location between the inlet opening and the first filter to form a first sub-compartment within the casing and severing the casing at a first location.
B01D 46/58 - Filtres ou procédés spécialement modifiés pour la séparation de particules dispersées dans des gaz ou des vapeurs avec plusieurs éléments filtrants, caractérisés par leur disposition relative montés en parallèle
B01D 46/00 - Filtres ou procédés spécialement modifiés pour la séparation de particules dispersées dans des gaz ou des vapeurs
B01F 23/231 - Mélange de gaz avec des liquides en introduisant des gaz dans des milieux liquides, p. ex. pour produire des liquides aérés par barbotage
B01F 27/2121 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins caractérisés par leurs arbres de rotation composés de parties interconnectées
B01F 27/88 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins avec des agitateurs tournant autour d'un axe sensiblement vertical avec une unité récipient-agitateur séparée qui est adaptée pour être couplée à un mécanisme d'entraînement
B01F 27/90 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins avec des agitateurs tournant autour d'un axe sensiblement vertical avec des palettes ou des bras
An instrument for processing and/or measuring a biological process comprises a sample processing system and an excitation source exhibiting a spectral function of output power or intensity verses wavelength of output power or intensity. The spectral function has a minima wavelength corresponding to a local minima value of the output power or intensity; a first maxima wavelength corresponding to a first local maxima of output power or intensity, the output power or intensity at the first local maxima being greater than the output power or intensity at any wavelength less than the minima wavelength; a second maxima wavelength corresponding to a second local maxima of output power or intensity, the output power or intensity at the second local maxima being greater than the output at any wavelength greater than the minima wavelength; the minima wavelength is between the first maxima wavelength and the second maxima wavelength.
G01N 21/27 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en utilisant la détection photo-électrique
92.
PURIFICATION CHEMISTRIES AND FORMATS FOR SANGER DNA SEQUENCING REACTIONS ON A MICRO-FLUIDICS DEVICE
According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.
This disclosure describes master mix compositions, nucleic acid amplification kits, methods of manufacturing master mix compositions, and methods of using master mix compositions that minimize or eliminate the negative effects of contaminant nucleic acids. Conventional master mix compositions often include contaminant nucleic acids.
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
94.
COMPOSITIONS AND METHODS FOR IMMUNE REPERTOIRE SEQUENCING
The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
C12Q 1/6881 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour le typage de tissu ou de cellule, p. ex. sondes d’antigène leucocytaire humain [HLA]
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
The charging stand kit for assembling a charging stand (1) for electrical pipettes (10) comprises a charging module (2) configured to hold an electrical pipette (10) to be charged, the charging module (2) comprising first and second sets of electrical contacts (7, 8) on first and second sides for connecting the charging module (2) electrically to adjacent parts (2, 3) of the charging stand (1), a first leg (3) having an upper end con- figured to be attached to the first side of the charging module (2) and comprising a set of electrical contacts (9) configured to contact the first set of electrical contacts (7) of the charging module (2), and a second leg (4) having an upper end config- ured to be attached to the second side of the charging module (2).
A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
A method for sequencing a nucleic acid template includes: (a) performing a first sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a first predetermined ordering of nucleotides and/or reagents to obtain a first sequencing result; (b) after the first sequencing process, performing a second sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a second predetermined ordering of nucleotides and/or reagents to obtain a second sequencing result, the second predetermined ordering of nucleotides and/or reagents being different from the first predetermined ordering of nucleotides and/or reagents and at least one of the first and second predetermined orderings of nucleotides and/or reagents being designed for repeat sequencing; and (c) determining a sequence of bases corresponding to at least a portion of the nucleic acid template using both the first sequencing result and the second sequencing result.
