The present disclosure relates to a genetically modified host cell having improved production of sulfurol and/or sulfurol precursors, wherein the host cell expresses one or more heterologous thiaminase I, thiaminase II and/or phosphatase enzymes and/or one or more heterologous thiamine pathway enzymes converting thiamine, 5-(2-hydroxyethyl)-4-methyl-1,3-thiazole-2-carboxylic phosphate acid (cTHZ-P), 5-(2-hydroxyethyl)-4-methyl-1,3-thiazole-2-carboxylic acid (cTHZ) and/or THZ-P to sulfurol and/or 5-(2-hydroxyethyl)-4-methyl-1,3-thiazole-2-carboxylic acid (cTHZ), whereby the production of the sulfurol in the genetically modified host cell is improved compared to an unmodified parent host cell. Chemical methods for production of sulfurol are also provided.
01 - Chemical and biological materials for industrial, scientific and agricultural use
03 - Cosmetics and toiletries; cleaning, bleaching, polishing and abrasive preparations
05 - Pharmaceutical, veterinary and sanitary products
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
Antioxidants for use in the manufacture of food products; Antioxidants for use in the manufacture of beverages; Antioxidants for use in the manufacture of food and beverages; Antioxidants for use in the manufacture of cosmetics; Chemical additives for use in the manufacture of cosmetics; Chemical compositions and materials for use in the manufacture of cosmetics; Chemical preparations for use in the manufacture of cosmetics; Chemical products for the manufacture of cosmetics; Chemical substances for use in the manufacture of scented cosmetics; Chemical substances for use in the manufacture of scented toiletries; Chemicals for use in the manufacture of cosmetics; Antimicrobial agents for use in manufacture; Antimicrobial preservatives for cosmetics; Antimicrobial preservatives for pharmaceuticals; Acids; Organic acids for industrial use; Organic acid salts; Flavour enhancers for foodstuffs; Flavour enhancers for food; Food preservatives; Preservatives for foodstuffs; Chemical additives for food; Additives (Chemical -) for food; Chemical additives for use in the manufacture of food; Chemical additives; Chemical additives for foodstuffs; Active chemical ingredients. Anti-aging cream; Anti-aging creams; Anti-ageing creams; Anti-aging creams [for cosmetic use]; Anti-aging moisturizers; Anti-aging moisturizers used as cosmetics; Anti-aging serum for cosmetic use; Anti-aging skincare preparations; Anti-ageing moisturiser; Anti-ageing serum; Anti-wrinkle cream; Anti-wrinkle creams; Anti-wrinkle cream [for cosmetic use]; Anti-wrinkle creams [for cosmetic use]; Wrinkle resistant creams; Wrinkle resistant cream; Wrinkle resistant creams [for cosmetic use]; Skin lighteners; Skin moisturiser; Skin cream; Skin moisturisers; Skin creams; Skin moisturizer; Creams for the skin; Skin moisturizers; Cosmetics for use on the skin; Skin conditioners; Skin fresheners; Dandruff shampoo; Dandruff shampoos, not for medical purposes; Fragrances; Scents; Fragrance preparations; Household fragrances; Body fragrances; Fragrance for household purposes. Anti-oxidant food supplements; Anti-oxidants for dietary use; Antioxidants; Acne cleansers [pharmaceutical preparations]; Acne creams [pharmaceutical preparations]; Acne medications; Acne treatment preparations; Acne medication; Antibacterial acne preparations; Anti-inflammatories; Preparations for the treatment of acne; Antibacterial preparations; Antibiotics; Anti-microbial preparations; Antifungal antibiotics; Anti-infective antibiotics; Antimicrobial mouthwashes; Anti-infectives; Bactericides; Antibacterial pharmaceuticals; Fungicides; Anti-fungal dermatological preparations for use on the nails; Vaginal antifungals; Antifungal medication; Antifungal creams for medical use; Antifungal preparations; Anti-cancer drugs; Anti-cancer preparations; Anticancer preparations; Pharmaceutical preparations for treating dandruff; Dandruff (Pharmaceutical preparations for treating -); Acids for pharmaceutical purposes. Food flavourings; Flavourings for foods.
The present disclosure relates to a genetically modified host cell having improved production of thiamine, wherein the host cell expresses one or more heterologous ThiO enzymes converting glycine into dehydroglycine (DHG) in the host cell and/or one or more heterologous Thil enzymes catalyzing the transfer of sulfur from IscS to the sulfur carrier protein This in the host cell, whereby the production of the thiamine in the genetically modified host cell is improved compared to an unmodified parent host cell.
The invention provides a genetically modified bacterial cell capable of improved iron-sulfur cluster delivery, characterized by a modified gene encoding a mutant Iron Sulfur Cluster Regulator (IscR) as well as one or more transgenes encoding polypeptides that enhance the biosynthesis of either biotin, lipoic acid or thiamine. The invention provides a method for producing either biotin, lipoic acid or thiamine using the genetically modified bacterium of the invention; as well as for the use of the genetically modified bacterial cell for either biotin, lipoic acid or thiamine production.
C12N 15/70 - Vectors or expression systems specially adapted for E. coli
C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
5.
MICROBIAL CELL FACTORIES PRODUCING VITAMIN B COMPOUNDS
The present disclosure relates to a genetically modified host cell having increased production of one or more vitamin B compounds, wherein the host cell is genetically modified by mutating one or more native polynucleotide constructs for reducing formation of a CRP-cAMP complex in the host cell and/or introducing one or more genetic alterations increasing the degradation and/or non-CRP binding of cAMP in the host cell; whereby the production of the vitamin B compound in the genetically modified host cell is increased compared to a parent host cell.
The invention relates to a genetically modified prokaryotic cell capable of improved iron-sulfur cluster delivery, characterized by a modified gene encoding a mutant Iron Sulfur Cluster Regulator (IscR) and one or more transgenes or upregulated endogenous genes encoding iron-sulfur (Fe—S) cluster polypeptides or proteins that catalyze complex radical-mediated molecular rearrangements, electron transfer, radical or non-redox reactions, sulfur donation or perform regulatory functions. The prokaryotic cells are characterized by enhanced activity of these iron-sulfur (Fe—S) cluster polypeptides, enhancing their respective functional capacity, and facilitating enhanced yields of compounds in free and protein-bound forms, including heme, hemoproteins, tetrapyrroles, B vitamins, amino acids, δ-aminolevulinic acid, biofuels, isoprenoids, pyrroloquinoline quinone, ammonia, indigo, or their precursors, whose biosynthesis depends on their activity. The invention further relates to a method for producing said compounds or their precursors using the genetically modified prokaryotic cell of the invention, and the use of the genetically modified prokaryotic cell.
The present inventio provides methods for producing biotin comprising cultivation of a genetically modified host cells comprising an operative metabolic pathway producing biotin comprising a transgene encoding a Type II biotin synthase (T2BioB), wherein a holo-protein of the T2BioB comprises per polypeptide chain a first [4Fe-4S] cluster (radical SAM (RS) cluster) coordinated to a CxxxCxxC motif in the polypeptide chain and a second [4Fe-4S] cluster; wherein the cultivation comprises at least one step of microaerobic cultivation provided for by limiting oxygen supply to the growth medium; and optionally recovering and/or isolating the biotin.
C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
C12N 1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factorsStimulation of growth by removal of a chemical compound
C12N 15/70 - Vectors or expression systems specially adapted for E. coli
C12N 15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
C12N 15/79 - Vectors or expression systems specially adapted for eukaryotic hosts
8.
CELL FACTORIES FOR IMPROVED PRODUCTION OF COMPOUNDS AND PROTEINS DEPENDENT ON IRON SULFUR CLUSTERS
The invention relates to a genetically modified prokaryotic cell capable of improved iron-sulfur cluster delivery, characterized by a modified gene encoding a mutant Iron Sulfur Cluster Regulator (IscR) as well as one or more transgenes or upregulated endogenous genes encoding iron-sulfur (Fe-S) cluster polypeptides or proteins that catalyze complex radical-mediated molecular rearrangements, electron transfer, radical or non-redox reactions, sulfur donation or perform regulatory functions. Prokaryotic cells of the invention are characterized by enhanced activity of these iron-sulfur (Fe-S) cluster polypeptides, thereby enhancing their respective functional capacity, as well as facilitating enhanced yields of diverse compounds in free and protein-bound forms, including heme, hemoproteins, tetrapyrroles, B vitamins, amino acids, δ-aminolevulinic acid, biofuels, isoprenoids, pyrroloquinoline quinone, ammonia, indigo or their precursors, whose biosynthesis depends on their activity. The invention further relates to a method for producing each of said compounds, or their precursors using the genetically modified prokaryotic cell of the invention; as well as the use of the genetically modified prokaryotic cell.
The invention provides a genetically modified bacterial cell capable of improved iron-sulfur cluster delivery, characterized by a modified gene encoding a mutant Iron Sulfur Cluster Regulator (IscR) as well as one or more transgenes encoding polypeptides that enhance the biosynthesis of either biotin, lipoic acid or thiamine. The invention provides a method for producing either biotin, lipoic acid or thiamine using the genetically modified bacterium of the invention; as well as for the use of the genetically modified bacterial cell for either biotin, lipoic acid or thiamine production.
C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
The invention provides a genetically modified bacterium for production of thiamine; where the bacterium is characterized by a transgene encoding a thiamine monophosphate phosphatase (TMP phosphatase having EC 3.1.3.-) as well as transgenes encoding polypeptides that catalyze steps in the thiamine pathway. The genetically modified bacterium is characterized by enhanced synthesis and release of thiamine into the extracellular environment. The invention further provides a method for producing thiamine using the genetically modified bacterium of the invention; as well as the use of the genetically modified bacterium for extracellular thiamine production.
The present invention relates to a regulatable gene expression construct comprising a nucleic acid molecule comprising two or more regulation sequences encoding respective RNA molecules comprising a riboswitch responsive to an effector compound, said riboswitch being operably linked to a respective coding region which encodes a respective modulator compound for modulating the action of a respective growth regulator compound and each said riboswitch in each regulation sequence being selected be responsive to the same effector compound to trigger expression of its respective modulator compound. The invention also relates to a method of using the regulatable gene expression construct for selecting from a metagenomic library a primary modulator compound which effects a chemical transformation of a substrate into said effector compound or transports said effector compound into a micro-organism comprising the regulatable gene expression construct.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
C12N 15/67 - General methods for enhancing the expression
C40B 40/02 - Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cellsLibraries contained in or displayed by vectors, e.g. plasmidsLibraries containing only microorganisms or vectors
C40B 40/06 - Libraries containing nucleotides or polynucleotides, or derivatives thereof