Abrégé

The present disclosure relates to a genetically modified host cell having improved production of thiamine, wherein the host cell expresses one or more heterologous ThiO enzymes converting glycine into dehydroglycine (DHG) in the host cell and/or one or more heterologous ThiI enzymes catalyzing the transfer of sulfur from IscS to the sulfur carrier protein ThiS in the host cell, whereby the production of the thiamine in the genetically modified host cell is improved compared to an unmodified parent host cell.

Classes IPC  ?

• C12P 17/16 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle contenant plusieurs hétérocycles
• A61K 31/51 - Thiamines, p. ex. vitamine B1
• A61K 35/741 - Probiotiques
• A61K 35/742 - Bactéries sporulées, p. ex. Bacillus coagulans, Bacillus subtilis, clostridium ou Lactobacillus sporogenes
• C07K 14/195 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de bactéries
• C12N 9/06 - Oxydoréductases (1.), p. ex. luciférase agissant sur des composés contenant de l'azote comme donneurs (1.4, 1.5, 1.7)
• C12N 9/10 - Transférases (2.)
• C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
• C12N 9/88 - Lyases (4.)

Abrégé

A liposomal preparation of biotin comprising a lipid bilayer membrane encapsulating an aqueous solution of biotin, thereby constituting a biotin containing liposome. Compositions comprising these liposomes and their cosmetic and medical uses in hair or skin diseases.

Classes IPC  ?

• A61K 8/14 - Liposomes
• A61K 8/19 - Cosmétiques ou préparations similaires pour la toilette caractérisés par la composition contenant des composés inorganiques
• A61K 8/34 - Alcools
• A61K 8/55 - Cosmétiques ou préparations similaires pour la toilette caractérisés par la composition contenant des composés organiques contenant du phosphore
• A61K 8/67 - Vitamines
• A61Q 5/00 - Préparations pour les soins des cheveux
• A61Q 5/12 - Préparations contenant des agents de conditionnement des cheveux
• A61Q 19/00 - Préparations pour les soins de la peau
• A61Q 19/02 - Préparations pour les soins de la peau pour décolorer ou blanchir la peau chimiquement
• A61Q 19/08 - Préparations contre le vieillissement

Abrégé

The present disclosure relates to a genetically modified host cell having improved production of sulfurol and/or sulfurol precursors, wherein the host cell expresses one or more heterologous thiaminase I, thiaminase II and/or phosphatase enzymes and/or one or more heterologous thiamine pathway enzymes converting thiamine, 5-(2-hydroxyethyl)-4-methyl-1,3-thiazole-2-carboxylic phosphate acid (cTHZ-P), 5-(2-hydroxyethyl)-4-methyl-1,3-thiazole-2-carboxylic acid (cTHZ) and/or THZ-P to sulfurol and/or 5-(2-hydroxyethyl)-4-methyl-1,3-thiazole-2-carboxylic acid (cTHZ), whereby the production of the sulfurol in the genetically modified host cell is improved compared to an unmodified parent host cell. Chemical methods for production of sulfurol are also provided.

Classes IPC  ?

• C12N 9/10 - Transférases (2.)
• C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
• C12N 9/16 - Hydrolases (3.) agissant sur les liaisons esters (3.1)
• C12N 9/78 - Hydrolases (3.) agissant sur les liaisons carbone-azote autres que les liaisons peptidiques (3.5)
• C12N 9/88 - Lyases (4.)
• C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
• C12P 17/16 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle contenant plusieurs hétérocycles

Produits et services

Antioxidants for use in the manufacture of food products; Antioxidants for use in the manufacture of beverages; Antioxidants for use in the manufacture of food and beverages; Antioxidants for use in the manufacture of cosmetics; Chemical additives for use in the manufacture of cosmetics; Chemical compositions and materials for use in the manufacture of cosmetics; Chemical preparations for use in the manufacture of cosmetics; Chemical products for the manufacture of cosmetics; Chemical substances for use in the manufacture of scented cosmetics; Chemical substances for use in the manufacture of scented toiletries; Chemicals for use in the manufacture of cosmetics; Antimicrobial agents for use in manufacture; Antimicrobial preservatives for cosmetics; Antimicrobial preservatives for pharmaceuticals; Acids; Organic acids for industrial use; Organic acid salts; Flavour enhancers for foodstuffs; Flavour enhancers for food; Food preservatives; Preservatives for foodstuffs; Chemical additives for food; Additives (Chemical -) for food; Chemical additives for use in the manufacture of food; Chemical additives; Chemical additives for foodstuffs; Active chemical ingredients. Anti-aging cream; Anti-aging creams; Anti-ageing creams; Anti-aging creams [for cosmetic use]; Anti-aging moisturizers; Anti-aging moisturizers used as cosmetics; Anti-aging serum for cosmetic use; Anti-aging skincare preparations; Anti-ageing moisturiser; Anti-ageing serum; Anti-wrinkle cream; Anti-wrinkle creams; Anti-wrinkle cream [for cosmetic use]; Anti-wrinkle creams [for cosmetic use]; Wrinkle resistant creams; Wrinkle resistant cream; Wrinkle resistant creams [for cosmetic use]; Skin lighteners; Skin moisturiser; Skin cream; Skin moisturisers; Skin creams; Skin moisturizer; Creams for the skin; Skin moisturizers; Cosmetics for use on the skin; Skin conditioners; Skin fresheners; Dandruff shampoo; Dandruff shampoos, not for medical purposes; Fragrances; Scents; Fragrance preparations; Household fragrances; Body fragrances; Fragrance for household purposes. Anti-oxidant food supplements; Anti-oxidants for dietary use; Antioxidants; Acne cleansers [pharmaceutical preparations]; Acne creams [pharmaceutical preparations]; Acne medications; Acne treatment preparations; Acne medication; Antibacterial acne preparations; Anti-inflammatories; Preparations for the treatment of acne; Antibacterial preparations; Antibiotics; Anti-microbial preparations; Antifungal antibiotics; Anti-infective antibiotics; Antimicrobial mouthwashes; Anti-infectives; Bactericides; Antibacterial pharmaceuticals; Fungicides; Anti-fungal dermatological preparations for use on the nails; Vaginal antifungals; Antifungal medication; Antifungal creams for medical use; Antifungal preparations; Anti-cancer drugs; Anti-cancer preparations; Anticancer preparations; Pharmaceutical preparations for treating dandruff; Dandruff (Pharmaceutical preparations for treating -); Acids for pharmaceutical purposes. Food flavourings; Flavourings for foods.

Abrégé

The present disclosure relates to a genetically modified host cell having improved production of thiamine, wherein the host cell expresses one or more heterologous ThiO enzymes converting glycine into dehydroglycine (DHG) in the host cell and/or one or more heterologous Thil enzymes catalyzing the transfer of sulfur from IscS to the sulfur carrier protein This in the host cell, whereby the production of the thiamine in the genetically modified host cell is improved compared to an unmodified parent host cell.

Classes IPC  ?

• C12N 9/06 - Oxydoréductases (1.), p. ex. luciférase agissant sur des composés contenant de l'azote comme donneurs (1.4, 1.5, 1.7)
• C12N 9/10 - Transférases (2.)
• C12P 17/16 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle contenant plusieurs hétérocycles

Abrégé

The invention provides a genetically modified bacterial cell capable of improved iron-sulfur cluster delivery, characterized by a modified gene encoding a mutant Iron Sulfur Cluster Regulator (IscR) as well as one or more transgenes encoding polypeptides that enhance the biosynthesis of either biotin, lipoic acid or thiamine. The invention provides a method for producing either biotin, lipoic acid or thiamine using the genetically modified bacterium of the invention; as well as for the use of the genetically modified bacterial cell for either biotin, lipoic acid or thiamine production.

Classes IPC  ?

• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C07K 14/245 - Escherichia (G)
• C12N 9/10 - Transférases (2.)
• C12N 9/88 - Lyases (4.)
• C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
• C12N 15/77 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour CorynebacteriumVecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora pour Brevibacterium

Abrégé

The present disclosure relates to a genetically modified host cell having increased production of one or more vitamin B compounds, wherein the host cell is genetically modified by mutating one or more native polynucleotide constructs for reducing formation of a CRP-cAMP complex in the host cell and/or introducing one or more genetic alterations increasing the degradation and/or non-CRP binding of cAMP in the host cell; whereby the production of the vitamin B compound in the genetically modified host cell is increased compared to a parent host cell.

Classes IPC  ?

• C12N 1/20 - BactériesLeurs milieux de culture
• C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes

Abrégé

The invention relates to a genetically modified prokaryotic cell capable of improved iron-sulfur cluster delivery, characterized by a modified gene encoding a mutant Iron Sulfur Cluster Regulator (IscR) and one or more transgenes or upregulated endogenous genes encoding iron-sulfur (Fe—S) cluster polypeptides or proteins that catalyze complex radical-mediated molecular rearrangements, electron transfer, radical or non-redox reactions, sulfur donation or perform regulatory functions. The prokaryotic cells are characterized by enhanced activity of these iron-sulfur (Fe—S) cluster polypeptides, enhancing their respective functional capacity, and facilitating enhanced yields of compounds in free and protein-bound forms, including heme, hemoproteins, tetrapyrroles, B vitamins, amino acids, δ-aminolevulinic acid, biofuels, isoprenoids, pyrroloquinoline quinone, ammonia, indigo, or their precursors, whose biosynthesis depends on their activity. The invention further relates to a method for producing said compounds or their precursors using the genetically modified prokaryotic cell of the invention, and the use of the genetically modified prokaryotic cell.

Classes IPC  ?

• C07K 14/245 - Escherichia (G)
• C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
• C12N 9/10 - Transférases (2.)
• C12N 9/88 - Lyases (4.)
• C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
• C12N 9/90 - Isomérases (5.)
• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C12N 9/16 - Hydrolases (3.) agissant sur les liaisons esters (3.1)
• C12N 9/24 - Hydrolases (3.) agissant sur les composés glycosyliques (3.2)

Abrégé

The present inventio provides methods for producing biotin comprising cultivation of a genetically modified host cells comprising an operative metabolic pathway producing biotin comprising a transgene encoding a Type II biotin synthase (T2BioB), wherein a holo-protein of the T2BioB comprises per polypeptide chain a first [4Fe-4S] cluster (radical SAM (RS) cluster) coordinated to a CxxxCxxC motif in the polypeptide chain and a second [4Fe-4S] cluster; wherein the cultivation comprises at least one step of microaerobic cultivation provided for by limiting oxygen supply to the growth medium; and optionally recovering and/or isolating the biotin.

Classes IPC  ?

• C12P 17/18 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle contenant plusieurs hétérocycles condensés entre eux ou condensés avec un système carbocyclique commun, p. ex. rifamycine
• C12N 9/10 - Transférases (2.)
• C12N 1/38 - Stimulation chimique de la croissance ou de l'activité par addition de composés chimiques qui ne sont pas des facteurs essentiels de croissanceStimulation de la croissance par élimination d'un composé chimique
• C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
• C12N 15/74 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes procaryotes autres que E. coli, p. ex. Lactobacillus, Micromonospora
• C12N 15/79 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes

Abrégé

The invention relates to a genetically modified prokaryotic cell capable of improved iron-sulfur cluster delivery, characterized by a modified gene encoding a mutant Iron Sulfur Cluster Regulator (IscR) as well as one or more transgenes or upregulated endogenous genes encoding iron-sulfur (Fe-S) cluster polypeptides or proteins that catalyze complex radical-mediated molecular rearrangements, electron transfer, radical or non-redox reactions, sulfur donation or perform regulatory functions. Prokaryotic cells of the invention are characterized by enhanced activity of these iron-sulfur (Fe-S) cluster polypeptides, thereby enhancing their respective functional capacity, as well as facilitating enhanced yields of diverse compounds in free and protein-bound forms, including heme, hemoproteins, tetrapyrroles, B vitamins, amino acids, δ-aminolevulinic acid, biofuels, isoprenoids, pyrroloquinoline quinone, ammonia, indigo or their precursors, whose biosynthesis depends on their activity. The invention further relates to a method for producing each of said compounds, or their precursors using the genetically modified prokaryotic cell of the invention; as well as the use of the genetically modified prokaryotic cell.

Classes IPC  ?

• C07K 14/245 - Escherichia (G)
• C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger

Abrégé

The invention provides a genetically modified bacterial cell capable of improved iron-sulfur cluster delivery, characterized by a modified gene encoding a mutant Iron Sulfur Cluster Regulator (IscR) as well as one or more transgenes encoding polypeptides that enhance the biosynthesis of either biotin, lipoic acid or thiamine. The invention provides a method for producing either biotin, lipoic acid or thiamine using the genetically modified bacterium of the invention; as well as for the use of the genetically modified bacterial cell for either biotin, lipoic acid or thiamine production.

Classes IPC  ?

• C07K 14/245 - Escherichia (G)
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 9/10 - Transférases (2.)
• C12N 9/88 - Lyases (4.)
• C12P 7/40 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carboxyle
• C12P 17/16 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle contenant plusieurs hétérocycles
• C12P 17/18 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle contenant plusieurs hétérocycles condensés entre eux ou condensés avec un système carbocyclique commun, p. ex. rifamycine

Abrégé

The invention provides a genetically modified bacterial cell capable of improved iron-sulfur cluster delivery, characterized by a modified gene encoding a mutant Iron Sulfur Cluster Regulator (IscR) as well as one or more transgenes encoding polypeptides that enhance the biosynthesis of either biotin, lipoic acid or thiamine. The invention provides a method for producing either biotin, lipoic acid or thiamine using the genetically modified bacterium of the invention; as well as for the use of the genetically modified bacterial cell for either biotin, lipoic acid or thiamine production.

Classes IPC  ?

• C07K 14/245 - Escherichia (G)
• C12P 17/18 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle contenant plusieurs hétérocycles condensés entre eux ou condensés avec un système carbocyclique commun, p. ex. rifamycine
• C12N 9/10 - Transférases (2.)
• C12N 9/88 - Lyases (4.)
• C12P 7/40 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carboxyle
• C12P 17/16 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle contenant plusieurs hétérocycles
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger

Abrégé

The invention provides a genetically modified bacterium for production of thiamine; where the bacterium is characterized by a transgene encoding a thiamine monophosphate phosphatase (TMP phosphatase having EC 3.1.3.-) as well as transgenes encoding polypeptides that catalyze steps in the thiamine pathway. The genetically modified bacterium is characterized by enhanced synthesis and release of thiamine into the extracellular environment. The invention further provides a method for producing thiamine using the genetically modified bacterium of the invention; as well as the use of the genetically modified bacterium for extracellular thiamine production.

Classes IPC  ?

• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• C12N 9/06 - Oxydoréductases (1.), p. ex. luciférase agissant sur des composés contenant de l'azote comme donneurs (1.4, 1.5, 1.7)
• C12N 9/10 - Transférases (2.)
• C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
• C12N 9/16 - Hydrolases (3.) agissant sur les liaisons esters (3.1)
• C12N 9/88 - Lyases (4.)
• C12N 15/55 - Hydrolases (3)
• C12P 17/16 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle contenant plusieurs hétérocycles

Abrégé

The present invention relates to a regulatable gene expression construct comprising a nucleic acid molecule comprising two or more regulation sequences encoding respective RNA molecules comprising a riboswitch responsive to an effector compound, said riboswitch being operably linked to a respective coding region which encodes a respective modulator compound for modulating the action of a respective growth regulator compound and each said riboswitch in each regulation sequence being selected be responsive to the same effector compound to trigger expression of its respective modulator compound. The invention also relates to a method of using the regulatable gene expression construct for selecting from a metagenomic library a primary modulator compound which effects a chemical transformation of a substrate into said effector compound or transports said effector compound into a micro-organism comprising the regulatable gene expression construct.

Classes IPC  ?

• C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
• C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
• C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
• C12N 15/67 - Méthodes générales pour favoriser l'expression
• C40B 40/02 - Bibliothèques contenues ou présentées dans des micro-organismes, p. ex. des bactéries ou des cellules animalesBibliothèques contenues ou présentées dans des vecteurs, p. ex. des plasmidesBibliothèques contenant uniquement des micro-organismes ou des vecteurs
• C40B 40/06 - Bibliothèques comprenant des nucléotides ou des polynucléotides ou leurs dérivés