Abstract

The present invention is in the field of enzyme and microorganism comprising such a modified enzyme to produce flavors, in particular raspberry ketone or zingerone. The enzymes of the invention are used for producing p-coumaric acid (PCA) from a tyrosine substrate, in particular from L-tyrosine. The invention also relates to microorganisms expressing such enzymes with Tyrosine Ammonia Lyase for producing flavors.

IPC Classes  ?

• C12N 15/78 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
• C07K 14/195 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria
• C12N 1/20 - BacteriaCulture media therefor
• C12N 9/88 - Lyases (4.)
• C12P 7/22 - Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic

Abstract

The present invention relates to the field involved in the production of phenylbutanone or phenylbutanone derivative compounds, such as frambinone or zingerone, and in particular strains genetically modified to express a benzalacetone reductase.

IPC Classes  ?

• C12P 7/26 - Ketones
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/88 - Lyases (4.)
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12N 15/78 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas

Abstract

The present invention relates to the field of the production of phenylpropanoid compounds, especially that of genetically modified strains for the production of phenylpropanoid compounds. In particular, the invention relates to a genetically modified strain of Pseudomonas putida comprising a mutated AroF-I gene encoding 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP), and to the use thereof for the synthesis of phenylpropanoid compounds, in particular coumaric acid or frambinone.

IPC Classes  ?

• C12N 1/20 - BacteriaCulture media therefor
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/10 - Transferases (2.)
• C12N 9/88 - Lyases (4.)
• C12N 15/78 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
• C12P 7/26 - Ketones
• C12P 7/42 - Hydroxy carboxylic acids
• C12P 17/06 - Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
• C12P 19/32 - Nucleotides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same-ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
• C12R 1/40 - Pseudomonas putida

Abstract

The present invention is in the field of genetically modified enzyme and microorganism comprising such a modified enzyme for the production of raspberry ketone or zingerone. The modified enzyme is a polyketide synthase (PKS) issued or derived from a wild type PKS. The modified PKS has the capability to produce vanillylidene acetone from feruloyl-CoA and/or 4-hydroxybenzalacetone from 4-coumaroyl-CoA in a more effective way as compared to wild type BAS or wild type PmPKS, in particular in recombinant bacteria strains.

IPC Classes  ?

• C12N 9/10 - Transferases (2.)
• C12N 1/20 - BacteriaCulture media therefor
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/88 - Lyases (4.)
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12P 7/28 - Acetone-containing products
• C12R 1/40 - Pseudomonas putida

Abstract

The present invention relates to the field involved in the production of phenylbutanone or phenylbutanone derivative compounds, such as frambinone or zingerone, and in particular strains genetically modified to express a benzalacetone reductase.

IPC Classes  ?

• C12N 9/88 - Lyases (4.)
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/10 - Transferases (2.)
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 15/78 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas

Abstract

The present invention relates to the field of the production of phenylpropanoid compounds, especially that of genetically modified strains for the production of phenylpropanoid compounds. In particular, the invention relates to a genetically modified strain of Pseudomonas putida comprising a mutated AroF-I gene encoding 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP), and to the use thereof for the synthesis of phenylpropanoid compounds, in particular coumaric acid or frambinone.

IPC Classes  ?

• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/10 - Transferases (2.)
• C12N 9/88 - Lyases (4.)
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12P 7/22 - Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
• C12P 7/42 - Hydroxy carboxylic acids
• C12P 7/26 - Ketones
• C12R 1/40 - Pseudomonas putida

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Chemicals used in industry, science and photography, as well as in agriculture, horticulture and forestry; Chemical reagents, other than for medical or veterinary purposes. Dietary supplements for humans and animals; Chemical preparations for medical or pharmaceutical purposes; Veterinary preparations; Pharmaceutical preparations. Engineering evaluations and appraisals in the fields of science and technology; Scientific and technical research; Design and development of computer hardware and software; Research and development for others; Technical project studies.

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Chemicals used in industry, science and photography, as well as in agriculture, horticulture and forestry; Chemical reagents, other than for medical or veterinary purposes. Pharmaceutical preparations; Veterinary preparations; Dietary supplements for humans and animals; Chemical preparations for medical or pharmaceutical purposes. Engineering evaluations and appraisals in the fields of science and technology; Scientific and technical research; Design and development of computer hardware and software; Research and development for others; Technical project studies.