In various aspects and embodiments, the present disclosure provides methods for making glycosylated products, as well as bacterial cells and uridine diphosphate (UDP)-dependent glycosyltransferase (UGT) enzymes useful for the same. In other aspects and embodiments, the disclosure provides methods for high yield and/or high purity recovery of such glycoside products from microbial cultures or cell free reactions. In various aspects and embodiments, the disclosure provides for whole cell bioconversion processes involving the glycosylation of a desired substrate, and/or the recovery of the glycosylated product at high yield and/or high purity.
C12P 19/60 - Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
The present invention provides host cells and methods for making mogrol glycosides, including Mogroside V (Mog. V), Mogroside VI (Mog. VI), Iso-Mogroside V (Isomog. V), siamenoside, and glycosylation products that are minor products in Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog. V, Mog. VI, and Isomog. V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog. V, Mog. VI, and Isomog. V, including food products, beverages, oral care products, sweeteners, and flavoring products.
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
4.
MICROBIAL PRODUCTION OF TRITERPENOIDS INCLUDING MOGROSIDES
The present invention provides host cells and methods for making mogrol glycosides, including Mogroside V (Mog. V), Mogroside VI (Mog. VI), Iso-Mogroside V (Isomog. V), and glycosylation products that are minor products in Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog. V, Mog. VI, and Isomog. V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog. V, Mog. VI, and Isomog. V, including food products, beverages, oral care products, sweeteners, and flavoring products.
C12P 19/60 - Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
The present disclosure relates to methods for producing oxygenated terpenoids, and preparation of compositions and formulations thereof. Polynucleotides, derivative enzymes, and host cells for use in such methods are also provided.
The present disclosure is based on development of engineered microbial cells that improve the microbial production of terpenoids compared to known processes by optimizing utilization of biosynthetic intermediates of the target terpenoids and/or promoting the cellular secreted production of target terpenoids.
The present disclosure relates, in part, to microbial hosts capable of synthesizing cis-3-hexenol, cis-3-hexenal, trans-3-hexenol, trans-3-hexenal, trans-2-hexenal, cis-2-hexenal and related compounds from hexanoic acid and methods for the preparation of cis-3-hexenol, cis-3-hexenal, trans-3-hexenol, trans-3-hexenal, trans-2-hexenal, cis-2-hexenal and related compounds.
The present disclosure in various aspects provides host cells and methods for making sesquiterpene components of cypriol oil or oud oil. In some embodiments, the host cells express one or more terpene synthase (TPS) enzymes. In some embodiments, the invention provides host cells producing oxidized sesquiterpene components of cypriol oil or oud oil. In these embodiments, the host cells further expresses one or more cytochrome P450 (CYP) enzymes.
In various aspects and embodiments, this disclosure provides methods and systems for making a chemical product by microbial fermentation. In embodiments, the method comprises providing a cell culture producing the chemical product from carbon substrate or by enzymatic bioconversion of a fed substrate. In some aspects and embodiments, the cells are cultured in a fermentation media in a bioreactor under conditions suitable for producing the chemical product, while continuously harvesting filtered fermentation media from the bioreactor and feeding the culture with fresh media while retaining cultured biomass. The chemical product is recovered from the filtered fermentation media and/or the retentate.
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
The present disclosure relates to microbial cells, engineered enzymes, and methods for producing mogrol or mogroside. The method comprises providing a microbial cell producing squalene and expressing a biosynthetic pathway that converts squalene to the mogrol or mogroside and culturing the microbial cell under conditions suitable for producing the mogrol or mogroside. In various embodiments, the methods disclosed herein produce squalene derivatives with less than about 40% by weight of off-pathway products cucurbitadienol, hydrolyzed 2,3;22,23-dioxidosqualene, and glycosylated 24,25-dihydroxycucurbitadienol.
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
The disclosure provides compositions and methods related to engineered microbial cells, enzymes, and methods for producing dammarenediol, as well as compounds derived from dammarenediol. Microbial host cells are engineered to express a heterologous biosynthetic pathway that produces dammarenediol, or a derivative thereof. The host cell can optionally express a heterologous uridine diphosphate-dependent glycosyltransferase (UGT) enzyme producing natural or non-natural glycosylated forms of dammarenediol, protopanaxadiol or protopanaxatriol.
The present disclosure relates, in part, to microbial hosts capable of synthesizing galactose and tagatose, and to methods for the preparation of galactose to tagatose using the microbial hosts.
The present disclosure in various aspects provides engineered enzymes and encoding polynucleotides, as well as host cells and methods for making rotundone and other terpenoids. For example, in various aspects, the invention provides engineered α-Guaiene Synthase (αGS) and Guaiene Oxidase (GO) enzymes that increase biosynthesis of rotundone from farnesyl diphosphate, and in certain embodiments substantially reduce biosynthesis of side products such as α-Bulnesene or oxygenated side products. In still other aspects, the invention provides engineered terpene synthase enzymes for directing biosynthesis toward a desired product, to thereby improve product profiles and/or product titers from terpene synthase reactions.
40 - Treatment of materials; recycling, air and water treatment,
41 - Education, entertainment, sporting and cultural services
42 - Scientific, technological and industrial services, research and design
Goods & Services
Business consulting in the field of synthetic biology,
biomanufacturing and biochemical engineering; business
consultancy and management services regarding marketing
activities and launching of new products for
commercialization purposes; business consulting regarding
scale-up of new product commercialization. Biomanufacturing for others, namely, manufacturing of
chemicals and natural products using biological organisms in
the manufacturing process; biomanufacturing for others,
namely, precision fermentation manufacturing of chemicals
using biological organisms in the manufacturing process;
biomanufacturing for others, namely, manufacturing of
chemicals using a manufacturing platform consisting of an
integrated production line that uses biological organisms in
the nature of engineered microbes and enzymes in the
manufacturing process to produce a wide variety of
chemicals; consulting in the field of manufacturing
utilizing synthetic biology; consulting in the field of
manufacturing utilizing synthetic biology for the purposes
of assisting in manufacturing process scale-up; providing
facilities for biomanufacturing and manufacturing utilizing
synthetic biology. Training in the field of synthetic biology, biomanufacturing
and biochemical engineering; business training regarding the
development, growth and scale-up of synthetic biology
manufacturing, engineering, design and development. Biochemical engineering services; product development;
product development in the fields of pharmaceuticals,
therapeutics, insect repellant, natural sweeteners, sugar
substitutes, dietetic sugar substitutes, food ingredients,
biomaterials, agricultural chemicals, bio-agrochemicals,
biomaterials, ingredients for beauty and wellness products,
consumer health and wellness products; development of new
technology for others in the field of synthetic biology;
research and development of technology in the field of
synthetic biology; technical consulting regarding the
technology of synthetic biology; technical consulting
regarding the technology of synthetic biology for the
purposes of assisting in engineering, product design and
product development scale-up; providing a website featuring
technology enabling users to design and develop new products
using synthetic biology; platform as a service services
(PaaS) featuring software for designing and developing new
products using synthetic biology.
stevia leaves, and provides enzymes, encoding polynucleotides, and host cells for use in these methods. The invention provides engineered enzymes and engineered host cells for producing steviol glycosylation products, such as RebM, at high purity and/or yield. The invention further provides methods of making products containing steviol glycosides, such as RebM, including food products, beverages, oral care products, sweeteners, and flavoring products.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
40 - Treatment of materials; recycling, air and water treatment,
41 - Education, entertainment, sporting and cultural services
42 - Scientific, technological and industrial services, research and design
Goods & Services
Business consulting in the field of synthetic biology,
biomanufacturing and biochemical engineering; new product
commercialization services; business consulting regarding
scale-up of new product commercialization. Biomanufacturing for others, namely, custom manufacturing of
chemicals and natural products using biological organisms in
the manufacturing process; biomanufacturing for others,
namely, precision fermentation custom manufacturing of
chemicals using biological organisms in the manufacturing
process; biomanufacturing for others, namely, custom
manufacturing of chemicals using a manufacturing platform
consisting of an integrated production line that uses
biological organisms in the nature of engineered microbes
and enzymes in the manufacturing process to produce a wide
variety of chemicals; consulting in the field of custom
manufacturing utilizing synthetic biology; consulting in the
field of custom manufacturing utilizing synthetic biology
for the purposes of assisting in manufacturing process
scale-up; providing facilities for rental of machines for
use in biomanufacturing and manufacturing utilizing
synthetic biology for the purpose of manufacturing
chemicals. Training in the field of synthetic biology, biomanufacturing
and biochemical engineering; business training regarding the
development, growth and scale-up of synthetic biology
manufacturing, engineering, design and development. Biochemical engineering services; product development;
product development in the fields of pharmaceuticals,
therapeutics, insect repellant, natural sweeteners, sugar
substitutes, dietetic sugar substitutes, food ingredients,
biomaterials, agricultural chemicals, bio-agrochemicals,
biomaterials, ingredients for beauty and wellness products,
consumer health and wellness products; development of new
technology for others in the field of synthetic biology;
research and development of technology in the field of
synthetic biology; technical consulting regarding the
technology of synthetic biology; technical consulting
regarding the technology of synthetic biology for the
purposes of assisting in engineering, product design and
product development scale-up; providing via website
technology that enables users to design and develop new
products using synthetic biology; platform as a service
services (PaaS) featuring software for designing and
developing new products using synthetic biology.
40 - Treatment of materials; recycling, air and water treatment,
41 - Education, entertainment, sporting and cultural services
42 - Scientific, technological and industrial services, research and design
Goods & Services
Business consulting in the field of synthetic biology,
biomanufacturing and biochemical engineering; new product
commercialization services; business consulting regarding
scale-up of new product commercialization. Biomanufacturing for others, namely, manufacturing of
chemicals and natural products using biological organisms in
the manufacturing process; biomanufacturing for others,
namely, precision fermentation manufacturing of chemicals
using biological organisms in the manufacturing process;
biomanufacturing for others, namely, manufacturing of
chemicals using a manufacturing platform consisting of an
integrated production line that uses biological organisms in
the nature of engineered microbes and enzymes in the
manufacturing process to produce a wide variety of
chemicals; consulting in the field of manufacturing
utilizing synthetic biology; consulting in the field of
manufacturing utilizing synthetic biology for the purposes
of assisting in manufacturing process scale-up; providing
facilities for biomanufacturing and manufacturing utilizing
synthetic biology. Training in the field of synthetic biology, biomanufacturing
and biochemical engineering; business training regarding the
development, growth and scale-up of synthetic biology
manufacturing, engineering, design and development. Biochemical engineering services; product development;
product development in the fields of pharmaceuticals,
therapeutics, insect repellant, natural sweeteners, sugar
substitutes, dietetic sugar substitutes, food ingredients,
biomaterials, agricultural chemicals, bio-agrochemicals,
biomaterials, ingredients for beauty and wellness products,
consumer health and wellness products; development of new
technology for others in the field of synthetic biology;
research and development of technology in the field of
synthetic biology; technical consulting regarding the
technology of synthetic biology; technical consulting
regarding the technology of synthetic biology for the
purposes of assisting in engineering, product design and
product development scale-up; providing via website
technology that enables users to design and develop new
products using synthetic biology; platform as a service
services (PaaS) featuring software for designing and
developing new products using synthetic biology.
The present disclosure relates to methods for producing oxygenated terpenoids, and preparation of compositions and formulations thereof. Polynucleotides, derivative enzymes, and host cells for use in such methods are also provided.
In various aspects and embodiments, the present disclosure provides methods for making glycosylated products, as well as bacterial cells and uridine diphosphate (UDP)-dependent glycosyltransferase (UGT) enzymes useful for the same. In other aspects and embodiments, the disclosure provides methods for high yield and/or high purity recovery of such glycoside products from microbial cultures or cell free reactions. In various aspects and embodiments, the disclosure provides for whole cell bioconversion processes involving the glycosylation of a desired substrate, and/or the recovery of the glycosylated product at high yield and/or high purity.
C12P 19/60 - Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
The present disclosure provides insect and/or arachnid repellent compositions, and methods of use thereof. The compositions disclosed herein comprise nootkatone and one or more fragrance agents and/or non-fragrance agents effective to for repelling the insects or arachnids. The fragrance or non-fragrance agents in various embodiments alter or mask human sweat odor, are effective to repel insects or arachnids, and/or alter or inhibit insect or arachnid olfactory systems.
A01N 35/06 - Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinoneDerivatives thereof, e.g. ketals
A01N 27/00 - Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
A01N 31/06 - Oxygen or sulfur directly attached to a cycloaliphatic ring system
A01N 43/24 - Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atom with two or more hetero atoms
A01N 37/18 - Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N, e.g. carboxylic acid amides or imidesThio-analogues thereof
A01N 37/42 - Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio-analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
A01N 65/28 - Myrtaceae [Myrtle family], e.g. teatree or clove
40 - Treatment of materials; recycling, air and water treatment,
41 - Education, entertainment, sporting and cultural services
42 - Scientific, technological and industrial services, research and design
Goods & Services
(1) Business consulting in the field of synthetic biology, biomanufacturing and biochemical engineering; business consultancy and management services regarding marketing activities and launching of new products for commercialization purposes; business consulting regarding scale-up of new product commercialization.
(2) Biomanufacturing for others, namely, manufacturing of chemicals and natural products using biological organisms in the manufacturing process; biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; biomanufacturing for others, namely, manufacturing of chemicals using a manufacturing platform consisting of an integrated production line that uses biological organisms in the nature of engineered microbes and enzymes in the manufacturing process to produce a wide variety of chemicals; consulting in the field of manufacturing utilizing synthetic biology; consulting in the field of manufacturing utilizing synthetic biology for the purposes of assisting in manufacturing process scale-up; providing facilities for biomanufacturing and manufacturing utilizing synthetic biology.
(3) Training in the field of synthetic biology, biomanufacturing and biochemical engineering; business training regarding the development, growth and scale-up of synthetic biology manufacturing, engineering, design and development.
(4) Biochemical engineering services; product development; product development in the fields of pharmaceuticals, therapeutics, insect repellant, natural sweeteners, sugar substitutes, dietetic sugar substitutes, food ingredients, biomaterials, agricultural chemicals, bio-agrochemicals, biomaterials, ingredients for beauty and wellness products, consumer health and wellness products; development of new technology for others in the field of synthetic biology; research and development of technology in the field of synthetic biology; technical consulting regarding the technology of synthetic biology; technical consulting regarding the technology of synthetic biology for the purposes of assisting in engineering, product design and product development scale-up; providing a website featuring technology enabling users to design and develop new products using synthetic biology; platform as a service services (PaaS) featuring software for designing and developing new products using synthetic biology.
27.
GENOME-MODIFIED BACTERIAL STRAINS FOR LARGE SCALE BIOPROCESSES
The present invention in various aspects and embodiments provides engineered bacterial strains having a modified genome that provides advantages in maintenance and biosynthetic processes when cultured at large scale. The present invention further provides methods for large-scale fermentation using the bacterial strains. In various embodiments, the strain engineering yields a reduction in cellular responses to micro-environmental stimuli imposed in large scale bioreactors.
40 - Treatment of materials; recycling, air and water treatment,
41 - Education, entertainment, sporting and cultural services
42 - Scientific, technological and industrial services, research and design
Goods & Services
(1) Business consulting in the field of synthetic biology, biomanufacturing and biochemical engineering; new product commercialization services; business consulting regarding scale-up of new product commercialization.
(2) Biomanufacturing for others, namely, manufacturing of chemicals and natural products using biological organisms in the manufacturing process; biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; biomanufacturing for others, namely, manufacturing of chemicals using a manufacturing platform consisting of an integrated production line that uses biological organisms in the nature of engineered microbes and enzymes in the manufacturing process to produce a wide variety of chemicals; consulting in the field of manufacturing utilizing synthetic biology; consulting in the field of manufacturing utilizing synthetic biology for the purposes of assisting in manufacturing process scale-up; providing facilities for biomanufacturing and manufacturing utilizing synthetic biology.
(3) Training in the field of synthetic biology, biomanufacturing and biochemical engineering; business training regarding the development, growth and scale-up of synthetic biology manufacturing, engineering, design and development.
(4) Biochemical engineering services; product development; product development in the fields of pharmaceuticals, therapeutics, insect repellant, natural sweeteners, sugar substitutes, dietetic sugar substitutes, food ingredients, biomaterials, agricultural chemicals, bio-agrochemicals, biomaterials, ingredients for beauty and wellness products, consumer health and wellness products; development of new technology for others in the field of synthetic biology; research and development of technology in the field of synthetic biology; technical consulting regarding the technology of synthetic biology; technical consulting regarding the technology of synthetic biology for the purposes of assisting in engineering, product design and product development scale-up; providing via website technology that enables users to design and develop new products using synthetic biology; platform as a service services (PaaS) featuring software for designing and developing new products using synthetic biology.
In various aspects and embodiments, this disclosure provides methods and systems for making a chemical product by microbial fermentation. In embodiments, the method comprises providing a cell culture producing the chemical product from carbon substrate or by enzymatic bioconversion of a fed substrate. In some aspects and embodiments, the cells are cultured in a fermentation media in a bioreactor under conditions suitable for producing the chemical product, while continuously harvesting filtered fermentation media from the bioreactor and feeding the culture with fresh media while retaining cultured biomass. The chemical product is recovered from the filtered fermentation media and/or the retentate.
C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
The present invention relates to the production of chemical species in bacterial host cells. Particularly, the present invention provides for the production of chemical species in Escherichia coli (E. coli) host cells that functionally express engineered P450 enzymes.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
The present disclosure provides methods and compositions for producing artemisinic acid, dihydroartemisinic acid or artemisinin. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of artemisinic acid, dihydroartemisinic acid or artemisinin. The present disclosure further provides methods of making pharmaceutical products containing artemisinic acid, dihydroartemisinic acid or artemisinin.
C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
C12N 15/52 - Genes encoding for enzymes or proenzymes
The present disclosure relates to microbial hosts and methods for biosynthesis of compositions comprising milk fat triglycerides. In one aspect, the present disclosure provides engineered host cells (e.g., microbial host cells) for producing triglycerides characteristic of dairy milk by microbial fermentation or bioconversion; the microbial cell expresses a biosynthetic pathway comprising at least one heterologous enzyme, where the biosynthetic pathway produces triglycerides having short chain fatty acids esterified at sn-3. The triglycerides comprises C4, C6, C12 and C18 fatty acids esterified at sn-3.
The present disclosure relates to microbial hosts and methods for biosynthesis of compositions comprising milk fat triglycerides. In one aspect, the present disclosure provides engineered host cells (e.g., microbial host cells) for producing triglycerides characteristic of dairy milk by microbial fermentation or bioconversion; the microbial cell expresses a biosynthetic pathway comprising at least one heterologous enzyme, where the biosynthetic pathway produces triglycerides having short chain fatty acids esterified at sn-3. The triglycerides comprises C4, C6, C12 and C18 fatty acids esterified at sn-3.
C12N 15/52 - Genes encoding for enzymes or proenzymes
A23C 9/12 - Fermented milk preparationsTreatment using microorganisms or enzymes
A23C 11/04 - Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing non-milk fats but no non-milk proteins
A23L 33/115 - Fatty acids or derivatives thereofFats or oils
C12P 7/6463 - Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
Goods & Services
Business consulting in the field of synthetic biology, biomanufacturing and biochemical engineering Biomanufacturing for others, namely, manufacturing of chemicals and natural products being taste and texture ingredients, flavors, industrial chemicals, pharmaceuticals and agricultural products being herbicides, pesticides and biostimulants using biological organisms in the manufacturing process; Biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; Biomanufacturing for others, namely, manufacturing of chemicals using a manufacturing platform consisting of an integrated production line that uses biological organisms in the nature of engineered microbes and enzymes in the manufacturing process to produce a wide variety of chemicals; manufacturing process consulting in the field of manufacturing utilizing synthetic biology; manufacturing process consulting in the field of manufacturing utilizing synthetic biology for the purposes of assisting in manufacturing process scale-up; providing specialized facilities for biomanufacturing and manufacturing utilizing synthetic biology Biochemical engineering services; product development in the fields of pharmaceuticals, therapeutics, insect repellant, natural sweeteners, sugar substitutes, dietetic sugar substitutes, food ingredients, biomaterials, agricultural chemicals, bio-agrochemicals, biomaterials, ingredients for beauty and wellness products, consumer health and wellness products; development of new technology for others in the field of synthetic biology; research and development of technology in the field of synthetic biology; technical technology consulting regarding the technology of synthetic biology; technical technology consulting regarding the technology of synthetic biology for the purposes of assisting in engineering, product design and product development scale-up; providing a website featuring technology enabling users to design and develop new products using synthetic biology; platform as a service services (PaaS) featuring computer software platforms for designing and developing new products using synthetic biology
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
Goods & Services
Business consulting in the field of synthetic biology, biomanufacturing and biochemical engineering Biomanufacturing for others, namely, manufacturing of chemicals and natural products being taste and texture ingredients, flavors, industrial chemicals, pharmaceuticals and agricultural products being herbicides, pesticides and biostimulants using biological organisms in the manufacturing process; Biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; Biomanufacturing for others, namely, manufacturing of chemicals using a manufacturing platform consisting of an integrated production line that uses biological organisms in the nature of engineered microbes and enzymes in the manufacturing process to produce a wide variety of chemicals; manufacturing process consulting in the field of manufacturing utilizing synthetic biology; manufacturing process consulting in the field of manufacturing utilizing synthetic biology for the purposes of assisting in manufacturing process scale-up; providing specialized facilities for biomanufacturing and manufacturing utilizing synthetic biology Biochemical engineering services; product development in the fields of pharmaceuticals, therapeutics, insect repellant, natural sweeteners, sugar substitutes, dietetic sugar substitutes, food ingredients, biomaterials, agricultural chemicals, bio-agrochemicals, biomaterials, ingredients for beauty and wellness products, consumer health and wellness products; development of new technology for others in the field of synthetic biology; research and development of technology in the field of synthetic biology; technical technology consulting regarding the technology of synthetic biology; technical technology consulting regarding the technology of synthetic biology for the purposes of assisting in engineering, product design and product development scale-up; providing a website featuring technology enabling users to design and develop new products using synthetic biology; platform as a service services (PaaS) featuring computer software platforms for designing and developing new products using synthetic biology
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
Goods & Services
Biomanufacturing for others, namely, manufacturing of chemicals and natural products using biological organisms in the manufacturing process; Biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; Biomanufacturing for others, namely, manufacturing of chemicals using a manufacturing platform consisting of an integrated production line that uses biological organisms in the nature of engineered microbes and enzymes in the manufacturing process to produce a wide variety of chemicals; manufacturing process consulting in the field of manufacturing utilizing synthetic biology; manufacturing process consulting in the field of manufacturing utilizing synthetic biology for the purposes of assisting in manufacturing process scale-up; providing specialized facilities for biomanufacturing and manufacturing utilizing synthetic biology Biochemical engineering services; product development; product development in the fields of pharmaceuticals, therapeutics, insect repellant, natural sweeteners, sugar substitutes, dietetic sugar substitutes, food ingredients, biomaterials, agricultural chemicals, bio-agrochemicals, biomaterials, ingredients for beauty and wellness products, consumer health and wellness products; development of new technology for others in the field of synthetic biology; research and development of technology in the field of synthetic biology; technical technology consulting regarding the technology of synthetic biology; technical technology consulting regarding the technology of synthetic biology for the purposes of assisting in engineering, product design and product development scale-up
38.
ENZYMES, CELLS, AND METHODS FOR PRODUCING LACTONES
The present disclosure relates to microbial hosts, enzymes, and methods for biosynthesis of lactones. The gamma- and delta-lactones are intramolecular esters of the corresponding hydroxy fatty acids, and they contribute to the aroma of fruits, butter, cheese, and other foods. Sustainable and scalable processes for synthesizing delta-dodecalactone, as well as other valuable lactones, are needed.
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
Goods & Services
Biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; Biomanufacturing for others, namely, manufacturing of natural products and chemicals using a technology platform for cell factory engineering that uses biological organisms in the manufacturing process Biochemical research and development; custom engineering of biological cells
The present invention relates to systems and methods for screening natural products such as secondary metabolites produced by engineered microbial strains.
The present disclosure provides methods and compositions for producing rotundone. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of rotundone. In some embodiments, the present disclosure provides microbial host cells for producing rotundone at high purity and/or yield, from either enzymatic transformation of α-guaiene, or from sugar or other carbon source. The present disclosure further provides methods of making products containing rotundone, including flavor or fragrance products, among others.
The present invention provides engineered cells and methods for making high purity steviol glycosides, including RebM. In some aspects, the present invention provides host cells, such as bacterial cells (including but not limited to E. coli), that are engineered to overexpress and/or delete or inactivate one or more steviol glycoside transport proteins. The bacterial cells selectively export RebM, or other specific combination of steviol glycosides, out of the cell to increase productivity and reduce production costs associated with downstream purification. Non-target steviol glycosides are not transported to the extracellular medium in significant amounts.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
40 - Treatment of materials; recycling, air and water treatment,
41 - Education, entertainment, sporting and cultural services
42 - Scientific, technological and industrial services, research and design
Goods & Services
Business consulting in the field of synthetic biology, biomanufacturing and biochemical engineering Biomanufacturing for others, namely, manufacturing of chemicals and natural products being taste and texture ingredients, flavors, industrial chemicals, pharmaceuticals and agricultural products being herbicides, pesticides and biostimulants using biological organisms in the manufacturing process; Biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; Biomanufacturing for others, namely, manufacturing of chemicals using a manufacturing platform consisting of an integrated production line that uses biological organisms in the nature of engineered microbes and enzymes in the manufacturing process to produce a wide variety of chemicals; manufacturing process consulting in the field of manufacturing utilizing synthetic biology; manufacturing process consulting in the field of manufacturing utilizing synthetic biology for the purposes of assisting in manufacturing process scale-up; providing specialized facilities for biomanufacturing and manufacturing utilizing synthetic biology Business training in the field of synthetic biology, biomanufacturing and biochemical engineering for emerging businesses in the biomanufacturing sector; business training regarding the development, growth and scale-up of synthetic biology manufacturing, engineering, design and development for emerging businesses in the biomanufacturing sector Biochemical engineering services; product development in the fields of pharmaceuticals, therapeutics, insect repellant, natural sweeteners, sugar substitutes, dietetic sugar substitutes, food ingredients, biomaterials, agricultural chemicals, bio-agrochemicals, biomaterials, ingredients for beauty and wellness products, consumer health and wellness products; development of new technology for others in the field of synthetic biology; research and development of technology in the field of synthetic biology; technical technology consulting regarding the technology of synthetic biology; technical technology consulting regarding the technology of synthetic biology for the purposes of assisting in engineering, product design and product development scale-up; providing a website featuring technology enabling users to design and develop new products using synthetic biology; platform as a service services (PaaS) featuring computer software platforms for designing and developing new products using synthetic biology
40 - Treatment of materials; recycling, air and water treatment,
41 - Education, entertainment, sporting and cultural services
42 - Scientific, technological and industrial services, research and design
Goods & Services
Business consulting in the field of synthetic biology, biomanufacturing and biochemical engineering Biomanufacturing for others, namely, manufacturing of chemicals and natural products being taste and texture ingredients, flavors, industrial chemicals, pharmaceuticals and agricultural products being herbicides, pesticides and biostimulants using biological organisms in the manufacturing process; Biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; Biomanufacturing for others, namely, manufacturing of chemicals using a manufacturing platform consisting of an integrated production line that uses biological organisms in the nature of engineered microbes and enzymes in the manufacturing process to produce a wide variety of chemicals; manufacturing process consulting in the field of manufacturing utilizing synthetic biology; manufacturing process consulting in the field of manufacturing utilizing synthetic biology for the purposes of assisting in manufacturing process scale-up; providing specialized facilities for biomanufacturing and manufacturing utilizing synthetic biology Business training in the field of synthetic biology, biomanufacturing and biochemical engineering for emerging businesses in the biomanufacturing sector; business training regarding the development, growth and scale-up of synthetic biology manufacturing, engineering, design and development for emerging businesses in the biomanufacturing sector Biochemical engineering services; product development in the fields of pharmaceuticals, therapeutics, insect repellant, natural sweeteners, sugar substitutes, dietetic sugar substitutes, food ingredients, biomaterials, agricultural chemicals, bio-agrochemicals, biomaterials, ingredients for beauty and wellness products, consumer health and wellness products; development of new technology for others in the field of synthetic biology; research and development of technology in the field of synthetic biology; technical technology consulting regarding the technology of synthetic biology; technical technology consulting regarding the technology of synthetic biology for the purposes of assisting in engineering, product design and product development scale-up; providing a website featuring technology enabling users to design and develop new products using synthetic biology; platform as a service services (PaaS) featuring computer software platforms for designing and developing new products using synthetic biology
45.
ENZYMES, HOST CELLS, AND METHODS FOR BIOSYNTHESIS OF DAMMARENEDIOL AND DERIVATIVES
The disclosure provides compositions and methods related to engineered microbial cells, enzymes, and methods for producing dammarenediol, as well as compounds derived from dammarenediol. Microbial host cells are engineered to express a heterologous biosynthetic pathway that produces dammarenediol, or a derivative thereof. The host cell can optionally express a heterologous uridine diphosphate-dependent glycosyltransferase (UGT) enzyme producing natural or non-natural glycosylated forms of dammarenediol, protopanaxadiol or protopanaxatriol.
Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog.V, Mog.VI, and Isomog.V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog.V, Mog.VI, and Isomog.V, including food products, beverages, oral care products, sweeteners, and flavoring products.
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
47.
Metabolic engineering for microbial production of terpenoid products
In various aspects and embodiments, the invention relates to bacterial strains and methods for making terpene and terpenoid products. The invention provides bacterial strains with improved carbon flux through the MEP pathway, to thereby increase terpene and/or terpenoid product yield by fermentation with carbon sources such as glucose.
The present disclosure in various aspects provides engineered enzymes and encoding polynucleotides, as well as host cells and methods for making rotundone and other terpenoids. For example, in various aspects, the invention provides engineered a-Guaiene Synthase (αGS) and Guaiene Oxidase (GO) enzymes that increase biosynthesis of rotundone from famesyl diphosphate, and in certain embodiments substantially reduce biosynthesis of side products such as α-Bulnesene or oxygenated side products. In still other aspects, the invention provides engineered terpene synthase enzymes for directing biosynthesis toward a desired product, to thereby improve product profiles and/or product titers from terpene synthase reactions.
The present disclosure provides insect and/or arachnid repellent compositions, and methods of use thereof. The compositions disclosed herein comprise nootkatone and one or more fragrance agents and/or non-fragrance agents effective to for repelling the insects or arachnids. The fragrance or non-fragrance agents in various embodiments alter or mask human sweat odor, are effective to repel insects or arachnids, and/or alter or inhibit insect or arachnid olfactory systems.
A01N 35/06 - Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinoneDerivatives thereof, e.g. ketals
A01N 43/20 - Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atom with one hetero atom three- or four-membered rings
A61K 31/122 - Ketones having the oxygen atom directly attached to a ring, e.g. quinones, vitamin K1, anthralin
In various aspects and embodiments, the present disclosure provides methods for making glycosylated products, as well as bacterial cells and uridine diphosphate (UDP)- dependent glycosyltransferase (UGT) enzymes useful for the same. In other aspects and embodiments, the disclosure provides methods for high yield and/or high purity recovery of such glycoside products from microbial cultures or cell free reactions. In various aspects and embodiments, the disclosure provides for whole cell bioconversion processes involving the glycosylation of a desired substrate, and/or the recovery of the glycosylated product at high yield and/or high purity.
In various aspects and embodiments, the present disclosure provides methods for making glycosylated products, as well as bacterial cells and uridine diphosphate (UDP)- dependent glycosyltransferase (UGT) enzymes useful for the same. In other aspects and embodiments, the disclosure provides methods for high yield and/or high purity recovery of such glycoside products from microbial cultures or cell free reactions. In various aspects and embodiments, the disclosure provides for whole cell bioconversion processes involving the glycosylation of a desired substrate, and/or the recovery of the glycosylated product at high yield and/or high purity.
In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced glycosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl transferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells.
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
53.
GENOME-MODIFIED BACTERIAL STRAINS FOR LARGE SCALE BIOPROCESSES
The present invention in various aspects and embodiments provides engineered bacterial strains having a modified genome that provides advantages in maintenance and biosynthetic processes when cultured at large scale. The present invention further provides methods for large-scale fermentation using the bacterial strains. In various embodiments, the strain engineering yields a reduction in cellular responses to micro-environmental stimuli imposed in large scale bioreactors.
In various aspects, the present invention provides uridine diphosphate-dependent glycosyltransferase (UGT) enzymes capable of catalyzing the transfer of a monosaccharide moiety from a NDP-sugar to the 3′ carbon of a sugar moiety of a substrate, such as a terpenoid glycan, thereby functioning as a “1-3 UGT.” In other aspects, the invention provides polynucleotides encoding the 1-3 UGT, and host cells comprising the same. In still other aspects, the invention provides methods for preparing glycosylated substrates, including steviol glycosides, using the enzyme and host cells of this disclosure.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
C12N 15/52 - Genes encoding for enzymes or proenzymes
The present disclosure relates to methods for producing oxygenated terpenoids, and preparation of compositions and formulations thereof. Polynucleotides, derivative enzymes, and host cells for use in such methods are also provided.
The present disclosure provides methods and compositions for producing artemisinic acid, dihydroartemisinic acid or artemisinin. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of artemisinic acid, dihydroartemisinic acid or artemisinin. The present disclosure further provides methods of making pharmaceutical products containing artemisinic acid, dihydroartemisinic acid or artemisinin.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
Chemical compound used as a sweetening ingredient in the
manufacture of food products and beverages. Dietary aid, namely, a low calorie-sweetening ingredient in
the nature of dietetic sugar substitutes for medical use
used in the manufacture of food products and beverages. Sweetening ingredient in the nature of sweeteners used in
the manufacture of food products and beverages; natural
sweetener; sugar substitutes; sweeteners principally
comprised of natural sweetener.
The present invention relates to methods for producing oxygenated terpenoids. Polynucleotides, derivative enzymes, and host cells for use in such methods are also provided.
40 - Treatment of materials; recycling, air and water treatment,
Goods & Services
Biomanufacturing for others, namely, manufacturing of natural products and chemicals using a technology platform for cell factory engineering that uses biological organisms in the manufacturing process; custom engineering of biological cells
40 - Treatment of materials; recycling, air and water treatment,
Goods & Services
Biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; biomanufacturing for others, namely, manufacturing of chemicals using a manufacturing platform consisting of an integrated production line that uses biological organisms in the nature of engineered microbes and enzymes in the manufacturing process to produce a wide variety of chemicals
The present disclosure provides methods and compositions for producing rotundone. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of rotundone. In some embodiments, the present disclosure provides microbial host cells for producing rotundone at high purity and/or yield, from either enzymatic transformation of α-guaiene, or from sugar or other carbon source. The present disclosure further provides methods of making products containing rotundone, including flavor or fragrance products, among others.
Aspects of the invention provide methods for producing one or more secondary metabolites from microbial culture. In various embodiments, the method comprises culturing a microbial cell producing a secondary metabolite for recovery from a bioreactor medium, the medium comprising an aqueous phase and an extraction phase. The composition of the extraction phase, and the relevant amount with respect to the aqueous phase, enhances production of the secondary metabolite from microbial cells and/or enhances extracellular transfer of the metabolite.
Siraitiagrosvenoriigrosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosyiation products, such as Mog.V, Mog.VI, and Isomog. V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog.V, Mog.VI, and Isomog. V, including food products, beverages, oral care products, sweeteners, and flavoring products.
The present invention provides host cells and methods for making mogrol glycosides, including Mogroside V (Mog.V), Mogroside VI (Mog.VI), Iso-Mogroside V (Isomog. V), siamenoside, and glycosyiation products that are minor products in Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosyiation products, such as Mog.V, Mog.VI, and Isomog. V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog.V, Mog.VI, and Isomog. V, including food products, beverages, oral care products, sweeteners, and flavoring products.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
Chemical compound used as a sweetening ingredient in the
manufacture of food products and beverages. Dietary aid, namely, a low calorie-sweetening ingredient
used in the manufacture of food products and beverages. Sweetening ingredient used in the manufacture of food
products and beverages.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
Chemical compound used as a sweetening ingredient in the
manufacture of food products and beverages. Dietary aid, namely, a low calorie-sweetening ingredient
used in the manufacture of food products and beverages. Sweetening ingredient used in the manufacture of food
products and beverages.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
(1) Chemical compound used as a sweetening ingredient in the manufacture of food products and beverages
(2) Dietary aid, namely, a low calorie-sweetening ingredient in the nature of dietetic sugar substitutes for medical use used in the manufacture of food products and beverages, none of the foregoing comprising or containing artificial sweeteners
(3) Sweetening ingredient in the nature of sweeteners used in the manufacture of food products and beverages, none of the foregoing comprising or containing artificial sweeteners
68.
Microbial production of triterpenoids including mogrosides
Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog. V, Mog. VI, and Isomog. V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog. V, Mog. VI, and Isomog. V, including food products, beverages, oral care products, sweeteners, and flavoring products.
C12P 19/60 - Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
stevia leaves, and provides enzymes, encoding polynucleotides, and host cells for use in these methods. The invention provides engineered enzymes and engineered host cells for producing steviol glycosylation products, such as RebM, at high purity and/or yield. The invention further provides methods of making products containing steviol glycosides, such as RebM, including food products, beverages, oral care products, sweeteners, and flavoring products.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
In various aspects, the present invention provides uridine diphosphate-dependent glycosyltransferase (UGT) enzymes capable of catalyzing the transfer of a monosaccharide moiety from a NDP-sugar to the 3′ carbon of a sugar moiety of a substrate, such as a terpenoid glycan, thereby functioning as a “1-3 UGT.” In other aspects, the invention provides polynucleotides encoding the 1-3 UGT, and host cells comprising the same. In still other aspects, the invention provides methods for preparing glycosylated substrates, including steviol glycosides, using the enzyme and host cells of this disclosure.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
C12N 15/52 - Genes encoding for enzymes or proenzymes
71.
Metabolic engineering for microbial production of terpenoid products
The invention relates to methods and bacterial strains for making terpene and terpenoid products, the bacterial strains having improved carbon pull through the MEP pathway and to a downstream recombinant synthesis pathway.
In various aspects, the present invention provides uridine diphosphate-dependent glycosyltransferase (UGT) enzymes capable of catalyzing the transfer of a monosaccharide moiety from a NDP-sugar to the 3' carbon of a sugar moiety of a substrate, such as a terpenoid glycan, thereby functioning as a "1-3 UGT." In other aspects, the invention provides polynucleotides encoding the 1-3 UGT, and host cells comprising the same. In still other aspects, the invention provides methods for preparing glycosylated substrates, including steviol glycosides, using the enzyme and host cells of this disclosure.
In various aspects, the present invention provides uridine diphosphate-dependent glycosyltransferase (UGT) enzymes capable of catalyzing the transfer of a monosaccharide moiety from a NDP-sugar to the 3' carbon of a sugar moiety of a substrate, such as a terpenoid glycan, thereby functioning as a "1-3 UGT." In other aspects, the invention provides polynucleotides encoding the 1-3 UGT, and host cells comprising the same. In still other aspects, the invention provides methods for preparing glycosylated substrates, including steviol glycosides, using the enzyme and host cells of this disclosure.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
Chemical compound used as a sweetening ingredient in the
manufacture of food products and beverages. Dietary aid, namely, a low calorie-sweetening ingredient
used in the manufacture of food products and beverages. Sweetening ingredient used in the manufacture of food
products and beverages.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
Chemical compound used as a sweetening ingredient in the
manufacture of food products and beverages. Dietary aid, namely, a low calorie-sweetening ingredient
used in the manufacture of food products and beverages. Sweetening ingredient used in the manufacture of food
products and beverages; natural sweetener; sugar
substitutes; sweeteners principally comprised of natural
sweetener.
76.
Metabolic engineering for microbial production of terpenoid products
In various aspects and embodiments, the invention relates to bacterial strains and methods for making terpene and terpenoid products. The invention provides bacterial strains with improved carbon flux through the MEP pathway, to thereby increase terpene and/or terpenoid product yield by fermentation with carbon sources such as glucose.
05 - Pharmaceutical, veterinary and sanitary products
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
Dietary aid, namely, a low calorie-sweetening ingredient in the nature of dietetic sugar substitutes for medical use used in the manufacture of food products and beverages Sweetening ingredient in the nature of natural sweeteners and sugar substitutes used in the manufacture of food products and beverages; natural sweetener; sugar substitutes; sweeteners principally comprised of natural sweetener
01 - Chemical and biological materials for industrial, scientific and agricultural use
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
Chemical compound, derived from plants, used as a sweetening ingredient in the manufacture of food products and beverages Sweetening ingredient in the nature of sweeteners used in the manufacture of food products and beverages, none of the foregoing comprising or containing artificial sweeteners
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Enzymes for scientific and research purposes; enzymes, namely, nucleases, for scientific or research use in connection with cell manipulation, cell transformation, genomic editing and genomic editing analysis; kits for gene editing comprising enzymes for scientific and research purposes
42 - Scientific, technological and industrial services, research and design
Goods & Services
Providing temporary use of non-downloadable software for designing and editing libraries of genetic cassettes; providing temporary use of non-downloadable software for designing and editing cells, genomes, and genetic cassettes
Escherichia coliYarrowialipolyticalipolytica). The enzymes include a heterologous cannabigerolic acid synthase as well as additional enzymes involved in the biosynthesis of cannabinoid precursors such as geranyl diphosphate, olivetol, olivetolic acid, divarin and/or divarinic acid. Methods are provided for producing C5-cannabinoids and/or C3-cannabinoids by fermentation of the recombinant host cell. Alternatively, cannabinoids can be produced by biotransformation of cannabinoid precursors in recombinant cells or by disrupted recombinant cells.
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
C12N 15/52 - Genes encoding for enzymes or proenzymes
30 - Basic staples, tea, coffee, baked goods and confectionery
Goods & Services
Sweetening ingredient in the nature of a sugar substitute used in the manufacture of food products and beverages; natural sweetener; sugar substitutes; sweeteners principally comprised of natural sweetener; none of the foregoing comprising or containing chemically synthesized sweeteners
84.
BIOSYNTHESIS AND RECOVERY OF SECONDARY METABOLITES
Aspects of the invention provide methods for producing one or more secondary metabolites from microbial culture. In various embodiments, the method comprises culturing a microbial cell producing a secondary metabolite for recovery from a bioreactor medium, the medium comprising an aqueous phase and an extraction phase. The composition of the extraction phase, and the relevant amount with respect to the aqueous phase, enhances production of the secondary metabolite from microbial cells and/or enhances extracellular transfer of the metabolite.
C12N 1/26 - Processes using, or culture media containing, hydrocarbons
C12P 1/04 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes by using bacteria
C12P 1/06 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes by using actinomycetales
C12P 7/00 - Preparation of oxygen-containing organic compounds
C12P 19/00 - Preparation of compounds containing saccharide radicals
C12P 23/00 - Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced glycosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl transferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells.
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
The present disclosure provides methods and compositions for producing rotundone. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of rotundone. In some embodiments, the present disclosure provides microbial host cells for producing rotundone at high purity and/or yield, from either enzymatic transformation of a- guaiene, or from sugar or other carbon source. The present disclosure further provides methods of making products containing rotundone, including flavor or fragrance products, among others.
In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced gly- cosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl trans- ferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells.
C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
C12P 19/00 - Preparation of compounds containing saccharide radicals
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
C12P 19/44 - Preparation of O-glycosides, e.g. glucosides
88.
PRODUCTION OF STEVIOL GLYCOSIDES THROUGH WHOLE CELL BIOTRANSFORMATION
In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced glycosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl transferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
C12P 19/44 - Preparation of O-glycosides, e.g. glucosides
C12P 19/00 - Preparation of compounds containing saccharide radicals
C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
89.
Metabolic engineering for microbial production of terpenoid products
The invention relates to methods and bacterial strains for making terpene and terpenoid products, the bacterial strains having improved carbon pull through the MEP pathway and to a downstream recombinant synthesis pathway.
The present invention is related to enzymatic pathways for production of carminic acid, host cells capable of production of carminic acid, and methods for the production of carminic acid and related compounds.
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
C07K 1/00 - General processes for the preparation of peptides
C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
C12N 1/15 - Fungi Culture media therefor modified by introduction of foreign genetic material
01 - Chemical and biological materials for industrial, scientific and agricultural use
09 - Scientific and electric apparatus and instruments
Goods & Services
Reagents and kits comprising reagents for scientific or research use in connection with cell manipulation, cell transformation, genomic editing and genomic editing analysis Scientific and laboratory apparatus and instruments for manipulating cells and editing genomes
01 - Chemical and biological materials for industrial, scientific and agricultural use
09 - Scientific and electric apparatus and instruments
Goods & Services
Reagents and kits comprising reagents for scientific or research use in connection with cell manipulation, cell transformation, genomic editing and genomic editing analysis Scientific and laboratory apparatus and instruments for manipulating cells and editing genomes
93.
ENZYMES, CELLS, AND METHODS FOR PRODUCTION OF 3-(4-FARNESYLOXYPHENYL)PROPIONIC ACID
The present disclosure provides microbial cells and methods of producing FOPPA resulting from unique biosynthetic pathways, including biosynthetic pathways based on the phenylalanine/tyrosine biosynthetic branch and biosynthetic pathways based on bacteria metabolism. In particular, the present invention provides methods of producing FOPPA in microbial cells. These methods provide a low-cost, sustainable, and environmentally friendly source for FOPPA.
C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
A61K 8/97 - Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof, of undetermined constitution from algae, fungi, lichens or plantsCosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof, of undetermined constitution from derivatives thereof
Siraitiagrosvenoriigrosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog. V, Mog. VI, and Isomog. V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog. V, Mog. VI, and Isomog. V, including food products, beverages, oral care products, sweeteners, and flavoring products.
The invention provides methods for making steviol glycosides, including RebM and glycosylation products that are minor products in stevia leaves, and provides enzymes, encoding polynucleotides, and host cells for use in these methods. The invention provides engineered enzymes and engineered host cells for producing steviol glycosylation products, such as RebM, at high purity and/or yield. The invention further provides methods of making products containing steviol glycosides, such as RebM, including food products, beverages, oral care products, sweeteners, and flavoring products.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
E. coliE. coli), that are engineered to overexpress and/or delete or inactivate one or more steviol glycoside transport proteins. The bacterial cells selectively export RebM, or other specific combination of steviol glycosides, out of the cell to increase productivity and reduce production costs associated with downstream purification. Non-target steviol glycosides are not transported to the extracellular medium in significant amounts.
C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
C12N 15/70 - Vectors or expression systems specially adapted for E. coli
99.
Metabolic engineering for microbial production of terpenoid products
In various aspects and embodiments, the invention relates to bacterial strains and methods for making terpene and terpenoid products. The invention provides bacterial strains with improved carbon flux through the MEP pathway, to thereby increase terpene and/or terpenoid product yield by fermentation with carbon sources such as glucose.
In various aspects and embodiments, the invention relates to bacterial strains and methods for making terpene and terpenoid products. The invention provides bacterial strains with improved carbon flux through the MEP pathway, to thereby increase terpene and/or terpenoid product yield by fermentation with carbon sources such as glucose.
C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
C12N 15/52 - Genes encoding for enzymes or proenzymes
