In various aspects and embodiments, the present disclosure provides methods for making glycosylated products, as well as bacterial cells and uridine diphosphate (UDP)-dependent glycosyltransferase (UGT) enzymes useful for the same. In other aspects and embodiments, the disclosure provides methods for high yield and/or high purity recovery of such glycoside products from microbial cultures or cell free reactions. In various aspects and embodiments, the disclosure provides for whole cell bioconversion processes involving the glycosylation of a desired substrate, and/or the recovery of the glycosylated product at high yield and/or high purity.
C12P 19/60 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un hétérocycle autre que saccharide ou à un système cyclique condensé contenant un hétérocycle autre que saccharide, p. ex. coumermycine, novobiocine
C12P 17/06 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle contenant un hétérocycle à six chaînons, p. ex. fluorescéine
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 15/81 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour champignons pour levures
The present invention provides host cells and methods for making mogrol glycosides, including Mogroside V (Mog. V), Mogroside VI (Mog. VI), Iso-Mogroside V (Isomog. V), siamenoside, and glycosylation products that are minor products in Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog. V, Mog. VI, and Isomog. V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog. V, Mog. VI, and Isomog. V, including food products, beverages, oral care products, sweeteners, and flavoring products.
C12P 17/02 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12P 19/18 - Préparation de composés contenant des radicaux saccharide préparés par action d'une transférase glycosylique, p. ex. alpha-, bêta- ou gamma-cyclodextrines
C12P 19/56 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un système cyclique condensé d'au moins trois carbocycles, p. ex. daunomycine, adriamycine
4.
MICROBIAL PRODUCTION OF TRITERPENOIDS INCLUDING MOGROSIDES
The present invention provides host cells and methods for making mogrol glycosides, including Mogroside V (Mog. V), Mogroside VI (Mog. VI), Iso-Mogroside V (Isomog. V), and glycosylation products that are minor products in Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog. V, Mog. VI, and Isomog. V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog. V, Mog. VI, and Isomog. V, including food products, beverages, oral care products, sweeteners, and flavoring products.
C12P 19/60 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un hétérocycle autre que saccharide ou à un système cyclique condensé contenant un hétérocycle autre que saccharide, p. ex. coumermycine, novobiocine
The present disclosure relates to methods for producing oxygenated terpenoids, and preparation of compositions and formulations thereof. Polynucleotides, derivative enzymes, and host cells for use in such methods are also provided.
The present disclosure is based on development of engineered microbial cells that improve the microbial production of terpenoids compared to known processes by optimizing utilization of biosynthetic intermediates of the target terpenoids and/or promoting the cellular secreted production of target terpenoids.
The present disclosure relates, in part, to microbial hosts capable of synthesizing cis-3-hexenol, cis-3-hexenal, trans-3-hexenol, trans-3-hexenal, trans-2-hexenal, cis-2-hexenal and related compounds from hexanoic acid and methods for the preparation of cis-3-hexenol, cis-3-hexenal, trans-3-hexenol, trans-3-hexenal, trans-2-hexenal, cis-2-hexenal and related compounds.
C12P 7/04 - Préparation de composés organiques contenant de l'oxygène contenant un groupe hydroxyle acycliques
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 9/16 - Hydrolases (3.) agissant sur les liaisons esters (3.1)
The present disclosure in various aspects provides host cells and methods for making sesquiterpene components of cypriol oil or oud oil. In some embodiments, the host cells express one or more terpene synthase (TPS) enzymes. In some embodiments, the invention provides host cells producing oxidized sesquiterpene components of cypriol oil or oud oil. In these embodiments, the host cells further expresses one or more cytochrome P450 (CYP) enzymes.
In various aspects and embodiments, this disclosure provides methods and systems for making a chemical product by microbial fermentation. In embodiments, the method comprises providing a cell culture producing the chemical product from carbon substrate or by enzymatic bioconversion of a fed substrate. In some aspects and embodiments, the cells are cultured in a fermentation media in a bioreactor under conditions suitable for producing the chemical product, while continuously harvesting filtered fermentation media from the bioreactor and feeding the culture with fresh media while retaining cultured biomass. The chemical product is recovered from the filtered fermentation media and/or the retentate.
C12P 19/18 - Préparation de composés contenant des radicaux saccharide préparés par action d'une transférase glycosylique, p. ex. alpha-, bêta- ou gamma-cyclodextrines
C12P 19/56 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un système cyclique condensé d'au moins trois carbocycles, p. ex. daunomycine, adriamycine
The present disclosure relates to microbial cells, engineered enzymes, and methods for producing mogrol or mogroside. The method comprises providing a microbial cell producing squalene and expressing a biosynthetic pathway that converts squalene to the mogrol or mogroside and culturing the microbial cell under conditions suitable for producing the mogrol or mogroside. In various embodiments, the methods disclosed herein produce squalene derivatives with less than about 40% by weight of off-pathway products cucurbitadienol, hydrolyzed 2,3;22,23-dioxidosqualene, and glycosylated 24,25-dihydroxycucurbitadienol.
C12P 19/18 - Préparation de composés contenant des radicaux saccharide préparés par action d'une transférase glycosylique, p. ex. alpha-, bêta- ou gamma-cyclodextrines
C12P 19/56 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un système cyclique condensé d'au moins trois carbocycles, p. ex. daunomycine, adriamycine
The disclosure provides compositions and methods related to engineered microbial cells, enzymes, and methods for producing dammarenediol, as well as compounds derived from dammarenediol. Microbial host cells are engineered to express a heterologous biosynthetic pathway that produces dammarenediol, or a derivative thereof. The host cell can optionally express a heterologous uridine diphosphate-dependent glycosyltransferase (UGT) enzyme producing natural or non-natural glycosylated forms of dammarenediol, protopanaxadiol or protopanaxatriol.
The present disclosure relates, in part, to microbial hosts capable of synthesizing galactose and tagatose, and to methods for the preparation of galactose to tagatose using the microbial hosts.
The present disclosure in various aspects provides engineered enzymes and encoding polynucleotides, as well as host cells and methods for making rotundone and other terpenoids. For example, in various aspects, the invention provides engineered α-Guaiene Synthase (αGS) and Guaiene Oxidase (GO) enzymes that increase biosynthesis of rotundone from farnesyl diphosphate, and in certain embodiments substantially reduce biosynthesis of side products such as α-Bulnesene or oxygenated side products. In still other aspects, the invention provides engineered terpene synthase enzymes for directing biosynthesis toward a desired product, to thereby improve product profiles and/or product titers from terpene synthase reactions.
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
41 - Éducation, divertissements, activités sportives et culturelles
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Business consulting in the field of synthetic biology,
biomanufacturing and biochemical engineering; business
consultancy and management services regarding marketing
activities and launching of new products for
commercialization purposes; business consulting regarding
scale-up of new product commercialization. Biomanufacturing for others, namely, manufacturing of
chemicals and natural products using biological organisms in
the manufacturing process; biomanufacturing for others,
namely, precision fermentation manufacturing of chemicals
using biological organisms in the manufacturing process;
biomanufacturing for others, namely, manufacturing of
chemicals using a manufacturing platform consisting of an
integrated production line that uses biological organisms in
the nature of engineered microbes and enzymes in the
manufacturing process to produce a wide variety of
chemicals; consulting in the field of manufacturing
utilizing synthetic biology; consulting in the field of
manufacturing utilizing synthetic biology for the purposes
of assisting in manufacturing process scale-up; providing
facilities for biomanufacturing and manufacturing utilizing
synthetic biology. Training in the field of synthetic biology, biomanufacturing
and biochemical engineering; business training regarding the
development, growth and scale-up of synthetic biology
manufacturing, engineering, design and development. Biochemical engineering services; product development;
product development in the fields of pharmaceuticals,
therapeutics, insect repellant, natural sweeteners, sugar
substitutes, dietetic sugar substitutes, food ingredients,
biomaterials, agricultural chemicals, bio-agrochemicals,
biomaterials, ingredients for beauty and wellness products,
consumer health and wellness products; development of new
technology for others in the field of synthetic biology;
research and development of technology in the field of
synthetic biology; technical consulting regarding the
technology of synthetic biology; technical consulting
regarding the technology of synthetic biology for the
purposes of assisting in engineering, product design and
product development scale-up; providing a website featuring
technology enabling users to design and develop new products
using synthetic biology; platform as a service services
(PaaS) featuring software for designing and developing new
products using synthetic biology.
stevia leaves, and provides enzymes, encoding polynucleotides, and host cells for use in these methods. The invention provides engineered enzymes and engineered host cells for producing steviol glycosylation products, such as RebM, at high purity and/or yield. The invention further provides methods of making products containing steviol glycosides, such as RebM, including food products, beverages, oral care products, sweeteners, and flavoring products.
C12P 19/56 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un système cyclique condensé d'au moins trois carbocycles, p. ex. daunomycine, adriamycine
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
41 - Éducation, divertissements, activités sportives et culturelles
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Business consulting in the field of synthetic biology,
biomanufacturing and biochemical engineering; new product
commercialization services; business consulting regarding
scale-up of new product commercialization. Biomanufacturing for others, namely, custom manufacturing of
chemicals and natural products using biological organisms in
the manufacturing process; biomanufacturing for others,
namely, precision fermentation custom manufacturing of
chemicals using biological organisms in the manufacturing
process; biomanufacturing for others, namely, custom
manufacturing of chemicals using a manufacturing platform
consisting of an integrated production line that uses
biological organisms in the nature of engineered microbes
and enzymes in the manufacturing process to produce a wide
variety of chemicals; consulting in the field of custom
manufacturing utilizing synthetic biology; consulting in the
field of custom manufacturing utilizing synthetic biology
for the purposes of assisting in manufacturing process
scale-up; providing facilities for rental of machines for
use in biomanufacturing and manufacturing utilizing
synthetic biology for the purpose of manufacturing
chemicals. Training in the field of synthetic biology, biomanufacturing
and biochemical engineering; business training regarding the
development, growth and scale-up of synthetic biology
manufacturing, engineering, design and development. Biochemical engineering services; product development;
product development in the fields of pharmaceuticals,
therapeutics, insect repellant, natural sweeteners, sugar
substitutes, dietetic sugar substitutes, food ingredients,
biomaterials, agricultural chemicals, bio-agrochemicals,
biomaterials, ingredients for beauty and wellness products,
consumer health and wellness products; development of new
technology for others in the field of synthetic biology;
research and development of technology in the field of
synthetic biology; technical consulting regarding the
technology of synthetic biology; technical consulting
regarding the technology of synthetic biology for the
purposes of assisting in engineering, product design and
product development scale-up; providing via website
technology that enables users to design and develop new
products using synthetic biology; platform as a service
services (PaaS) featuring software for designing and
developing new products using synthetic biology.
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
41 - Éducation, divertissements, activités sportives et culturelles
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Business consulting in the field of synthetic biology,
biomanufacturing and biochemical engineering; new product
commercialization services; business consulting regarding
scale-up of new product commercialization. Biomanufacturing for others, namely, manufacturing of
chemicals and natural products using biological organisms in
the manufacturing process; biomanufacturing for others,
namely, precision fermentation manufacturing of chemicals
using biological organisms in the manufacturing process;
biomanufacturing for others, namely, manufacturing of
chemicals using a manufacturing platform consisting of an
integrated production line that uses biological organisms in
the nature of engineered microbes and enzymes in the
manufacturing process to produce a wide variety of
chemicals; consulting in the field of manufacturing
utilizing synthetic biology; consulting in the field of
manufacturing utilizing synthetic biology for the purposes
of assisting in manufacturing process scale-up; providing
facilities for biomanufacturing and manufacturing utilizing
synthetic biology. Training in the field of synthetic biology, biomanufacturing
and biochemical engineering; business training regarding the
development, growth and scale-up of synthetic biology
manufacturing, engineering, design and development. Biochemical engineering services; product development;
product development in the fields of pharmaceuticals,
therapeutics, insect repellant, natural sweeteners, sugar
substitutes, dietetic sugar substitutes, food ingredients,
biomaterials, agricultural chemicals, bio-agrochemicals,
biomaterials, ingredients for beauty and wellness products,
consumer health and wellness products; development of new
technology for others in the field of synthetic biology;
research and development of technology in the field of
synthetic biology; technical consulting regarding the
technology of synthetic biology; technical consulting
regarding the technology of synthetic biology for the
purposes of assisting in engineering, product design and
product development scale-up; providing via website
technology that enables users to design and develop new
products using synthetic biology; platform as a service
services (PaaS) featuring software for designing and
developing new products using synthetic biology.
The present disclosure relates to methods for producing oxygenated terpenoids, and preparation of compositions and formulations thereof. Polynucleotides, derivative enzymes, and host cells for use in such methods are also provided.
In various aspects and embodiments, the present disclosure provides methods for making glycosylated products, as well as bacterial cells and uridine diphosphate (UDP)-dependent glycosyltransferase (UGT) enzymes useful for the same. In other aspects and embodiments, the disclosure provides methods for high yield and/or high purity recovery of such glycoside products from microbial cultures or cell free reactions. In various aspects and embodiments, the disclosure provides for whole cell bioconversion processes involving the glycosylation of a desired substrate, and/or the recovery of the glycosylated product at high yield and/or high purity.
C12P 19/60 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un hétérocycle autre que saccharide ou à un système cyclique condensé contenant un hétérocycle autre que saccharide, p. ex. coumermycine, novobiocine
The present disclosure provides insect and/or arachnid repellent compositions, and methods of use thereof. The compositions disclosed herein comprise nootkatone and one or more fragrance agents and/or non-fragrance agents effective to for repelling the insects or arachnids. The fragrance or non-fragrance agents in various embodiments alter or mask human sweat odor, are effective to repel insects or arachnids, and/or alter or inhibit insect or arachnid olfactory systems.
A01N 35/06 - Biocides, produits repoussant ou attirant les animaux nuisibles, ou régulateurs de croissance des végétaux, contenant des composés organiques comportant un atome de carbone possédant deux liaisons à des hétéro-atomes, avec au plus une liaison à un halogène, p. ex. un radical aldéhyde contenant des groupes cétone ou thiocétone faisant partie d'un cycle, p. ex. cyclohexanone, quinoneLeurs dérivés, p. ex. cétals
A01N 27/00 - Biocides, produits repoussant ou attirant les animaux nuisibles, ou régulateurs de croissance des végétaux, contenant des hydrocarbures
A01N 31/06 - Biocides, produits repoussant ou attirant les animaux nuisibles, ou régulateurs de croissance des végétaux, contenant des composés organiques de l'oxygène ou du soufre l'atome d'oxygène ou de soufre étant lié directement à un système cyclique cycloaliphatique
A01N 43/24 - Biocides, produits repoussant ou attirant les animaux nuisibles, ou régulateurs de croissance des végétaux, contenant des composés hétérocycliques comportant des cycles avec un ou plusieurs atomes d'oxygène ou de soufre comme uniques hétéro-atomes du cycle avec plusieurs hétéro-atomes
A01N 37/18 - Biocides, produits repoussant ou attirant les animaux nuisibles, ou régulateurs de croissance des végétaux, contenant des composés organiques comportant un atome de carbone possédant trois liaisons à des hétéro-atomes, avec au plus deux liaisons à un halogène, p. ex. acides carboxyliques contenant le groupe —CO—N, p. ex. amides ou imides d'acide carboxyliqueLeurs thio-analogues
A01N 37/42 - Biocides, produits repoussant ou attirant les animaux nuisibles, ou régulateurs de croissance des végétaux, contenant des composés organiques comportant un atome de carbone possédant trois liaisons à des hétéro-atomes, avec au plus deux liaisons à un halogène, p. ex. acides carboxyliques contenant dans le même squelette carboné un groupe carboxylique ou un thio-analogue, ou un de leurs dérivés, et un atome de carbone ne comportant que deux liaisons à des hétéro-atomes, avec au plus une liaison à un halogène, p. ex. acides cétocarboxyliques
A01N 65/28 - Myrtaceae [famille du myrte], p. ex. cajeputier ou clou de girofle
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
41 - Éducation, divertissements, activités sportives et culturelles
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
(1) Business consulting in the field of synthetic biology, biomanufacturing and biochemical engineering; business consultancy and management services regarding marketing activities and launching of new products for commercialization purposes; business consulting regarding scale-up of new product commercialization.
(2) Biomanufacturing for others, namely, manufacturing of chemicals and natural products using biological organisms in the manufacturing process; biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; biomanufacturing for others, namely, manufacturing of chemicals using a manufacturing platform consisting of an integrated production line that uses biological organisms in the nature of engineered microbes and enzymes in the manufacturing process to produce a wide variety of chemicals; consulting in the field of manufacturing utilizing synthetic biology; consulting in the field of manufacturing utilizing synthetic biology for the purposes of assisting in manufacturing process scale-up; providing facilities for biomanufacturing and manufacturing utilizing synthetic biology.
(3) Training in the field of synthetic biology, biomanufacturing and biochemical engineering; business training regarding the development, growth and scale-up of synthetic biology manufacturing, engineering, design and development.
(4) Biochemical engineering services; product development; product development in the fields of pharmaceuticals, therapeutics, insect repellant, natural sweeteners, sugar substitutes, dietetic sugar substitutes, food ingredients, biomaterials, agricultural chemicals, bio-agrochemicals, biomaterials, ingredients for beauty and wellness products, consumer health and wellness products; development of new technology for others in the field of synthetic biology; research and development of technology in the field of synthetic biology; technical consulting regarding the technology of synthetic biology; technical consulting regarding the technology of synthetic biology for the purposes of assisting in engineering, product design and product development scale-up; providing a website featuring technology enabling users to design and develop new products using synthetic biology; platform as a service services (PaaS) featuring software for designing and developing new products using synthetic biology.
27.
GENOME-MODIFIED BACTERIAL STRAINS FOR LARGE SCALE BIOPROCESSES
The present invention in various aspects and embodiments provides engineered bacterial strains having a modified genome that provides advantages in maintenance and biosynthetic processes when cultured at large scale. The present invention further provides methods for large-scale fermentation using the bacterial strains. In various embodiments, the strain engineering yields a reduction in cellular responses to micro-environmental stimuli imposed in large scale bioreactors.
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
41 - Éducation, divertissements, activités sportives et culturelles
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
(1) Business consulting in the field of synthetic biology, biomanufacturing and biochemical engineering; new product commercialization services; business consulting regarding scale-up of new product commercialization.
(2) Biomanufacturing for others, namely, manufacturing of chemicals and natural products using biological organisms in the manufacturing process; biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; biomanufacturing for others, namely, manufacturing of chemicals using a manufacturing platform consisting of an integrated production line that uses biological organisms in the nature of engineered microbes and enzymes in the manufacturing process to produce a wide variety of chemicals; consulting in the field of manufacturing utilizing synthetic biology; consulting in the field of manufacturing utilizing synthetic biology for the purposes of assisting in manufacturing process scale-up; providing facilities for biomanufacturing and manufacturing utilizing synthetic biology.
(3) Training in the field of synthetic biology, biomanufacturing and biochemical engineering; business training regarding the development, growth and scale-up of synthetic biology manufacturing, engineering, design and development.
(4) Biochemical engineering services; product development; product development in the fields of pharmaceuticals, therapeutics, insect repellant, natural sweeteners, sugar substitutes, dietetic sugar substitutes, food ingredients, biomaterials, agricultural chemicals, bio-agrochemicals, biomaterials, ingredients for beauty and wellness products, consumer health and wellness products; development of new technology for others in the field of synthetic biology; research and development of technology in the field of synthetic biology; technical consulting regarding the technology of synthetic biology; technical consulting regarding the technology of synthetic biology for the purposes of assisting in engineering, product design and product development scale-up; providing via website technology that enables users to design and develop new products using synthetic biology; platform as a service services (PaaS) featuring software for designing and developing new products using synthetic biology.
In various aspects and embodiments, this disclosure provides methods and systems for making a chemical product by microbial fermentation. In embodiments, the method comprises providing a cell culture producing the chemical product from carbon substrate or by enzymatic bioconversion of a fed substrate. In some aspects and embodiments, the cells are cultured in a fermentation media in a bioreactor under conditions suitable for producing the chemical product, while continuously harvesting filtered fermentation media from the bioreactor and feeding the culture with fresh media while retaining cultured biomass. The chemical product is recovered from the filtered fermentation media and/or the retentate.
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
B01D 69/02 - Membranes semi-perméables destinées aux procédés ou aux appareils de séparation, caractérisées par leur forme, leur structure ou leurs propriétésProcédés spécialement adaptés à leur fabrication caractérisées par leurs propriétés
The present invention relates to the production of chemical species in bacterial host cells. Particularly, the present invention provides for the production of chemical species in Escherichia coli (E. coli) host cells that functionally express engineered P450 enzymes.
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12P 19/56 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un système cyclique condensé d'au moins trois carbocycles, p. ex. daunomycine, adriamycine
The present disclosure provides methods and compositions for producing artemisinic acid, dihydroartemisinic acid or artemisinin. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of artemisinic acid, dihydroartemisinic acid or artemisinin. The present disclosure further provides methods of making pharmaceutical products containing artemisinic acid, dihydroartemisinic acid or artemisinin.
C12P 17/18 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle contenant plusieurs hétérocycles condensés entre eux ou condensés avec un système carbocyclique commun, p. ex. rifamycine
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
The present disclosure relates to microbial hosts and methods for biosynthesis of compositions comprising milk fat triglycerides. In one aspect, the present disclosure provides engineered host cells (e.g., microbial host cells) for producing triglycerides characteristic of dairy milk by microbial fermentation or bioconversion; the microbial cell expresses a biosynthetic pathway comprising at least one heterologous enzyme, where the biosynthetic pathway produces triglycerides having short chain fatty acids esterified at sn-3. The triglycerides comprises C4, C6, C12 and C18 fatty acids esterified at sn-3.
The present disclosure relates to microbial hosts and methods for biosynthesis of compositions comprising milk fat triglycerides. In one aspect, the present disclosure provides engineered host cells (e.g., microbial host cells) for producing triglycerides characteristic of dairy milk by microbial fermentation or bioconversion; the microbial cell expresses a biosynthetic pathway comprising at least one heterologous enzyme, where the biosynthetic pathway produces triglycerides having short chain fatty acids esterified at sn-3. The triglycerides comprises C4, C6, C12 and C18 fatty acids esterified at sn-3.
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
A23C 9/12 - Préparations à base de lait fermentéTraitements utilisant des micro-organismes ou des enzymes
A23C 11/04 - Succédanés du lait, p. ex. compositions pour remplacer le lait dans le café au lait contenant au moins un composé d'origine non laitière comme source de matières grasses ou de protéines contenant des matières grasses d'origine non laitière mais pas de protéines d'origine non laitière
A23L 33/115 - Acides gras ou leurs dérivésGraisses ou huiles
C12P 7/6463 - Glycérides obtenus à partir de micro-organismes, p. ex. huile d’organismes unicellulaires
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Business consulting in the field of synthetic biology, biomanufacturing and biochemical engineering Biomanufacturing for others, namely, manufacturing of chemicals and natural products being taste and texture ingredients, flavors, industrial chemicals, pharmaceuticals and agricultural products being herbicides, pesticides and biostimulants using biological organisms in the manufacturing process; Biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; Biomanufacturing for others, namely, manufacturing of chemicals using a manufacturing platform consisting of an integrated production line that uses biological organisms in the nature of engineered microbes and enzymes in the manufacturing process to produce a wide variety of chemicals; manufacturing process consulting in the field of manufacturing utilizing synthetic biology; manufacturing process consulting in the field of manufacturing utilizing synthetic biology for the purposes of assisting in manufacturing process scale-up; providing specialized facilities for biomanufacturing and manufacturing utilizing synthetic biology Biochemical engineering services; product development in the fields of pharmaceuticals, therapeutics, insect repellant, natural sweeteners, sugar substitutes, dietetic sugar substitutes, food ingredients, biomaterials, agricultural chemicals, bio-agrochemicals, biomaterials, ingredients for beauty and wellness products, consumer health and wellness products; development of new technology for others in the field of synthetic biology; research and development of technology in the field of synthetic biology; technical technology consulting regarding the technology of synthetic biology; technical technology consulting regarding the technology of synthetic biology for the purposes of assisting in engineering, product design and product development scale-up; providing a website featuring technology enabling users to design and develop new products using synthetic biology; platform as a service services (PaaS) featuring computer software platforms for designing and developing new products using synthetic biology
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Business consulting in the field of synthetic biology, biomanufacturing and biochemical engineering Biomanufacturing for others, namely, manufacturing of chemicals and natural products being taste and texture ingredients, flavors, industrial chemicals, pharmaceuticals and agricultural products being herbicides, pesticides and biostimulants using biological organisms in the manufacturing process; Biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; Biomanufacturing for others, namely, manufacturing of chemicals using a manufacturing platform consisting of an integrated production line that uses biological organisms in the nature of engineered microbes and enzymes in the manufacturing process to produce a wide variety of chemicals; manufacturing process consulting in the field of manufacturing utilizing synthetic biology; manufacturing process consulting in the field of manufacturing utilizing synthetic biology for the purposes of assisting in manufacturing process scale-up; providing specialized facilities for biomanufacturing and manufacturing utilizing synthetic biology Biochemical engineering services; product development in the fields of pharmaceuticals, therapeutics, insect repellant, natural sweeteners, sugar substitutes, dietetic sugar substitutes, food ingredients, biomaterials, agricultural chemicals, bio-agrochemicals, biomaterials, ingredients for beauty and wellness products, consumer health and wellness products; development of new technology for others in the field of synthetic biology; research and development of technology in the field of synthetic biology; technical technology consulting regarding the technology of synthetic biology; technical technology consulting regarding the technology of synthetic biology for the purposes of assisting in engineering, product design and product development scale-up; providing a website featuring technology enabling users to design and develop new products using synthetic biology; platform as a service services (PaaS) featuring computer software platforms for designing and developing new products using synthetic biology
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Biomanufacturing for others, namely, manufacturing of chemicals and natural products using biological organisms in the manufacturing process; Biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; Biomanufacturing for others, namely, manufacturing of chemicals using a manufacturing platform consisting of an integrated production line that uses biological organisms in the nature of engineered microbes and enzymes in the manufacturing process to produce a wide variety of chemicals; manufacturing process consulting in the field of manufacturing utilizing synthetic biology; manufacturing process consulting in the field of manufacturing utilizing synthetic biology for the purposes of assisting in manufacturing process scale-up; providing specialized facilities for biomanufacturing and manufacturing utilizing synthetic biology Biochemical engineering services; product development; product development in the fields of pharmaceuticals, therapeutics, insect repellant, natural sweeteners, sugar substitutes, dietetic sugar substitutes, food ingredients, biomaterials, agricultural chemicals, bio-agrochemicals, biomaterials, ingredients for beauty and wellness products, consumer health and wellness products; development of new technology for others in the field of synthetic biology; research and development of technology in the field of synthetic biology; technical technology consulting regarding the technology of synthetic biology; technical technology consulting regarding the technology of synthetic biology for the purposes of assisting in engineering, product design and product development scale-up
38.
ENZYMES, CELLS, AND METHODS FOR PRODUCING LACTONES
The present disclosure relates to microbial hosts, enzymes, and methods for biosynthesis of lactones. The gamma- and delta-lactones are intramolecular esters of the corresponding hydroxy fatty acids, and they contribute to the aroma of fruits, butter, cheese, and other foods. Sustainable and scalable processes for synthesizing delta-dodecalactone, as well as other valuable lactones, are needed.
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; Biomanufacturing for others, namely, manufacturing of natural products and chemicals using a technology platform for cell factory engineering that uses biological organisms in the manufacturing process Biochemical research and development; custom engineering of biological cells
The present invention relates to systems and methods for screening natural products such as secondary metabolites produced by engineered microbial strains.
A01H 3/04 - Procédés de modification des phénotypes par traitement au moyen de produits chimiques
C12Q 1/02 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des micro-organismes viables
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
C12N 1/12 - Algues unicellulairesLeurs milieux de culture
The present disclosure provides methods and compositions for producing rotundone. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of rotundone. In some embodiments, the present disclosure provides microbial host cells for producing rotundone at high purity and/or yield, from either enzymatic transformation of α-guaiene, or from sugar or other carbon source. The present disclosure further provides methods of making products containing rotundone, including flavor or fragrance products, among others.
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
The present invention provides engineered cells and methods for making high purity steviol glycosides, including RebM. In some aspects, the present invention provides host cells, such as bacterial cells (including but not limited to E. coli), that are engineered to overexpress and/or delete or inactivate one or more steviol glycoside transport proteins. The bacterial cells selectively export RebM, or other specific combination of steviol glycosides, out of the cell to increase productivity and reduce production costs associated with downstream purification. Non-target steviol glycosides are not transported to the extracellular medium in significant amounts.
C12P 19/56 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un système cyclique condensé d'au moins trois carbocycles, p. ex. daunomycine, adriamycine
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
41 - Éducation, divertissements, activités sportives et culturelles
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Business consulting in the field of synthetic biology, biomanufacturing and biochemical engineering Biomanufacturing for others, namely, manufacturing of chemicals and natural products being taste and texture ingredients, flavors, industrial chemicals, pharmaceuticals and agricultural products being herbicides, pesticides and biostimulants using biological organisms in the manufacturing process; Biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; Biomanufacturing for others, namely, manufacturing of chemicals using a manufacturing platform consisting of an integrated production line that uses biological organisms in the nature of engineered microbes and enzymes in the manufacturing process to produce a wide variety of chemicals; manufacturing process consulting in the field of manufacturing utilizing synthetic biology; manufacturing process consulting in the field of manufacturing utilizing synthetic biology for the purposes of assisting in manufacturing process scale-up; providing specialized facilities for biomanufacturing and manufacturing utilizing synthetic biology Business training in the field of synthetic biology, biomanufacturing and biochemical engineering for emerging businesses in the biomanufacturing sector; business training regarding the development, growth and scale-up of synthetic biology manufacturing, engineering, design and development for emerging businesses in the biomanufacturing sector Biochemical engineering services; product development in the fields of pharmaceuticals, therapeutics, insect repellant, natural sweeteners, sugar substitutes, dietetic sugar substitutes, food ingredients, biomaterials, agricultural chemicals, bio-agrochemicals, biomaterials, ingredients for beauty and wellness products, consumer health and wellness products; development of new technology for others in the field of synthetic biology; research and development of technology in the field of synthetic biology; technical technology consulting regarding the technology of synthetic biology; technical technology consulting regarding the technology of synthetic biology for the purposes of assisting in engineering, product design and product development scale-up; providing a website featuring technology enabling users to design and develop new products using synthetic biology; platform as a service services (PaaS) featuring computer software platforms for designing and developing new products using synthetic biology
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
41 - Éducation, divertissements, activités sportives et culturelles
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Business consulting in the field of synthetic biology, biomanufacturing and biochemical engineering Biomanufacturing for others, namely, manufacturing of chemicals and natural products being taste and texture ingredients, flavors, industrial chemicals, pharmaceuticals and agricultural products being herbicides, pesticides and biostimulants using biological organisms in the manufacturing process; Biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; Biomanufacturing for others, namely, manufacturing of chemicals using a manufacturing platform consisting of an integrated production line that uses biological organisms in the nature of engineered microbes and enzymes in the manufacturing process to produce a wide variety of chemicals; manufacturing process consulting in the field of manufacturing utilizing synthetic biology; manufacturing process consulting in the field of manufacturing utilizing synthetic biology for the purposes of assisting in manufacturing process scale-up; providing specialized facilities for biomanufacturing and manufacturing utilizing synthetic biology Business training in the field of synthetic biology, biomanufacturing and biochemical engineering for emerging businesses in the biomanufacturing sector; business training regarding the development, growth and scale-up of synthetic biology manufacturing, engineering, design and development for emerging businesses in the biomanufacturing sector Biochemical engineering services; product development in the fields of pharmaceuticals, therapeutics, insect repellant, natural sweeteners, sugar substitutes, dietetic sugar substitutes, food ingredients, biomaterials, agricultural chemicals, bio-agrochemicals, biomaterials, ingredients for beauty and wellness products, consumer health and wellness products; development of new technology for others in the field of synthetic biology; research and development of technology in the field of synthetic biology; technical technology consulting regarding the technology of synthetic biology; technical technology consulting regarding the technology of synthetic biology for the purposes of assisting in engineering, product design and product development scale-up; providing a website featuring technology enabling users to design and develop new products using synthetic biology; platform as a service services (PaaS) featuring computer software platforms for designing and developing new products using synthetic biology
45.
ENZYMES, HOST CELLS, AND METHODS FOR BIOSYNTHESIS OF DAMMARENEDIOL AND DERIVATIVES
The disclosure provides compositions and methods related to engineered microbial cells, enzymes, and methods for producing dammarenediol, as well as compounds derived from dammarenediol. Microbial host cells are engineered to express a heterologous biosynthetic pathway that produces dammarenediol, or a derivative thereof. The host cell can optionally express a heterologous uridine diphosphate-dependent glycosyltransferase (UGT) enzyme producing natural or non-natural glycosylated forms of dammarenediol, protopanaxadiol or protopanaxatriol.
Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog.V, Mog.VI, and Isomog.V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog.V, Mog.VI, and Isomog.V, including food products, beverages, oral care products, sweeteners, and flavoring products.
C12P 17/02 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12P 19/18 - Préparation de composés contenant des radicaux saccharide préparés par action d'une transférase glycosylique, p. ex. alpha-, bêta- ou gamma-cyclodextrines
C12P 19/56 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un système cyclique condensé d'au moins trois carbocycles, p. ex. daunomycine, adriamycine
47.
Metabolic engineering for microbial production of terpenoid products
In various aspects and embodiments, the invention relates to bacterial strains and methods for making terpene and terpenoid products. The invention provides bacterial strains with improved carbon flux through the MEP pathway, to thereby increase terpene and/or terpenoid product yield by fermentation with carbon sources such as glucose.
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12P 7/40 - Préparation de composés organiques contenant de l'oxygène contenant un groupe carboxyle
C12P 17/02 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle
48.
ENZYMES, HOST CELLS, AND METHODS FOR PRODUCTION OF ROTUNDONE AND OTHER TERPENOIDS
The present disclosure in various aspects provides engineered enzymes and encoding polynucleotides, as well as host cells and methods for making rotundone and other terpenoids. For example, in various aspects, the invention provides engineered a-Guaiene Synthase (αGS) and Guaiene Oxidase (GO) enzymes that increase biosynthesis of rotundone from famesyl diphosphate, and in certain embodiments substantially reduce biosynthesis of side products such as α-Bulnesene or oxygenated side products. In still other aspects, the invention provides engineered terpene synthase enzymes for directing biosynthesis toward a desired product, to thereby improve product profiles and/or product titers from terpene synthase reactions.
The present disclosure provides insect and/or arachnid repellent compositions, and methods of use thereof. The compositions disclosed herein comprise nootkatone and one or more fragrance agents and/or non-fragrance agents effective to for repelling the insects or arachnids. The fragrance or non-fragrance agents in various embodiments alter or mask human sweat odor, are effective to repel insects or arachnids, and/or alter or inhibit insect or arachnid olfactory systems.
A01N 35/06 - Biocides, produits repoussant ou attirant les animaux nuisibles, ou régulateurs de croissance des végétaux, contenant des composés organiques comportant un atome de carbone possédant deux liaisons à des hétéro-atomes, avec au plus une liaison à un halogène, p. ex. un radical aldéhyde contenant des groupes cétone ou thiocétone faisant partie d'un cycle, p. ex. cyclohexanone, quinoneLeurs dérivés, p. ex. cétals
A01N 43/20 - Biocides, produits repoussant ou attirant les animaux nuisibles, ou régulateurs de croissance des végétaux, contenant des composés hétérocycliques comportant des cycles avec un ou plusieurs atomes d'oxygène ou de soufre comme uniques hétéro-atomes du cycle avec un hétéro-atome des cycles à trois ou quatre chaînons
A61K 31/122 - Cétones ayant l'atome d'oxygène lié directement à un cycle, p. ex. quinones, vitamine K1, anthraline
In various aspects and embodiments, the present disclosure provides methods for making glycosylated products, as well as bacterial cells and uridine diphosphate (UDP)- dependent glycosyltransferase (UGT) enzymes useful for the same. In other aspects and embodiments, the disclosure provides methods for high yield and/or high purity recovery of such glycoside products from microbial cultures or cell free reactions. In various aspects and embodiments, the disclosure provides for whole cell bioconversion processes involving the glycosylation of a desired substrate, and/or the recovery of the glycosylated product at high yield and/or high purity.
C12N 1/15 - ChampignonsLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 1/19 - LevuresLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
In various aspects and embodiments, the present disclosure provides methods for making glycosylated products, as well as bacterial cells and uridine diphosphate (UDP)- dependent glycosyltransferase (UGT) enzymes useful for the same. In other aspects and embodiments, the disclosure provides methods for high yield and/or high purity recovery of such glycoside products from microbial cultures or cell free reactions. In various aspects and embodiments, the disclosure provides for whole cell bioconversion processes involving the glycosylation of a desired substrate, and/or the recovery of the glycosylated product at high yield and/or high purity.
C12N 1/15 - ChampignonsLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 1/19 - LevuresLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced glycosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl transferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells.
C12P 19/18 - Préparation de composés contenant des radicaux saccharide préparés par action d'une transférase glycosylique, p. ex. alpha-, bêta- ou gamma-cyclodextrines
53.
GENOME-MODIFIED BACTERIAL STRAINS FOR LARGE SCALE BIOPROCESSES
The present invention in various aspects and embodiments provides engineered bacterial strains having a modified genome that provides advantages in maintenance and biosynthetic processes when cultured at large scale. The present invention further provides methods for large-scale fermentation using the bacterial strains. In various embodiments, the strain engineering yields a reduction in cellular responses to micro-environmental stimuli imposed in large scale bioreactors.
In various aspects, the present invention provides uridine diphosphate-dependent glycosyltransferase (UGT) enzymes capable of catalyzing the transfer of a monosaccharide moiety from a NDP-sugar to the 3′ carbon of a sugar moiety of a substrate, such as a terpenoid glycan, thereby functioning as a “1-3 UGT.” In other aspects, the invention provides polynucleotides encoding the 1-3 UGT, and host cells comprising the same. In still other aspects, the invention provides methods for preparing glycosylated substrates, including steviol glycosides, using the enzyme and host cells of this disclosure.
C12P 19/56 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un système cyclique condensé d'au moins trois carbocycles, p. ex. daunomycine, adriamycine
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
The present disclosure relates to methods for producing oxygenated terpenoids, and preparation of compositions and formulations thereof. Polynucleotides, derivative enzymes, and host cells for use in such methods are also provided.
The present disclosure provides methods and compositions for producing artemisinic acid, dihydroartemisinic acid or artemisinin. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of artemisinic acid, dihydroartemisinic acid or artemisinin. The present disclosure further provides methods of making pharmaceutical products containing artemisinic acid, dihydroartemisinic acid or artemisinin.
C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
C12P 17/02 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
C12P 9/00 - Préparation de composés organiques contenant un métal ou un atome autre que H, N, C, O, S ou un halogène
C12P 15/00 - Préparation de composés contenant au moins trois carbocycles condensés
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
30 - Aliments de base, thé, café, pâtisseries et confiseries
Produits et services
Chemical compound used as a sweetening ingredient in the
manufacture of food products and beverages. Dietary aid, namely, a low calorie-sweetening ingredient in
the nature of dietetic sugar substitutes for medical use
used in the manufacture of food products and beverages. Sweetening ingredient in the nature of sweeteners used in
the manufacture of food products and beverages; natural
sweetener; sugar substitutes; sweeteners principally
comprised of natural sweetener.
The present invention relates to methods for producing oxygenated terpenoids. Polynucleotides, derivative enzymes, and host cells for use in such methods are also provided.
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
Produits et services
Biomanufacturing for others, namely, manufacturing of natural products and chemicals using a technology platform for cell factory engineering that uses biological organisms in the manufacturing process; custom engineering of biological cells
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
Produits et services
Biomanufacturing for others, namely, precision fermentation manufacturing of chemicals using biological organisms in the manufacturing process; biomanufacturing for others, namely, manufacturing of chemicals using a manufacturing platform consisting of an integrated production line that uses biological organisms in the nature of engineered microbes and enzymes in the manufacturing process to produce a wide variety of chemicals
The present disclosure provides methods and compositions for producing rotundone. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of rotundone. In some embodiments, the present disclosure provides microbial host cells for producing rotundone at high purity and/or yield, from either enzymatic transformation of α-guaiene, or from sugar or other carbon source. The present disclosure further provides methods of making products containing rotundone, including flavor or fragrance products, among others.
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
Aspects of the invention provide methods for producing one or more secondary metabolites from microbial culture. In various embodiments, the method comprises culturing a microbial cell producing a secondary metabolite for recovery from a bioreactor medium, the medium comprising an aqueous phase and an extraction phase. The composition of the extraction phase, and the relevant amount with respect to the aqueous phase, enhances production of the secondary metabolite from microbial cells and/or enhances extracellular transfer of the metabolite.
Siraitiagrosvenoriigrosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosyiation products, such as Mog.V, Mog.VI, and Isomog. V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog.V, Mog.VI, and Isomog. V, including food products, beverages, oral care products, sweeteners, and flavoring products.
The present invention provides host cells and methods for making mogrol glycosides, including Mogroside V (Mog.V), Mogroside VI (Mog.VI), Iso-Mogroside V (Isomog. V), siamenoside, and glycosyiation products that are minor products in Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosyiation products, such as Mog.V, Mog.VI, and Isomog. V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog.V, Mog.VI, and Isomog. V, including food products, beverages, oral care products, sweeteners, and flavoring products.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
30 - Aliments de base, thé, café, pâtisseries et confiseries
Produits et services
Chemical compound used as a sweetening ingredient in the
manufacture of food products and beverages. Dietary aid, namely, a low calorie-sweetening ingredient
used in the manufacture of food products and beverages. Sweetening ingredient used in the manufacture of food
products and beverages.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
30 - Aliments de base, thé, café, pâtisseries et confiseries
Produits et services
Chemical compound used as a sweetening ingredient in the
manufacture of food products and beverages. Dietary aid, namely, a low calorie-sweetening ingredient
used in the manufacture of food products and beverages. Sweetening ingredient used in the manufacture of food
products and beverages.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
30 - Aliments de base, thé, café, pâtisseries et confiseries
Produits et services
(1) Chemical compound used as a sweetening ingredient in the manufacture of food products and beverages
(2) Dietary aid, namely, a low calorie-sweetening ingredient in the nature of dietetic sugar substitutes for medical use used in the manufacture of food products and beverages, none of the foregoing comprising or containing artificial sweeteners
(3) Sweetening ingredient in the nature of sweeteners used in the manufacture of food products and beverages, none of the foregoing comprising or containing artificial sweeteners
68.
Microbial production of triterpenoids including mogrosides
Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog. V, Mog. VI, and Isomog. V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog. V, Mog. VI, and Isomog. V, including food products, beverages, oral care products, sweeteners, and flavoring products.
C12P 19/60 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un hétérocycle autre que saccharide ou à un système cyclique condensé contenant un hétérocycle autre que saccharide, p. ex. coumermycine, novobiocine
stevia leaves, and provides enzymes, encoding polynucleotides, and host cells for use in these methods. The invention provides engineered enzymes and engineered host cells for producing steviol glycosylation products, such as RebM, at high purity and/or yield. The invention further provides methods of making products containing steviol glycosides, such as RebM, including food products, beverages, oral care products, sweeteners, and flavoring products.
C12P 19/56 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un système cyclique condensé d'au moins trois carbocycles, p. ex. daunomycine, adriamycine
In various aspects, the present invention provides uridine diphosphate-dependent glycosyltransferase (UGT) enzymes capable of catalyzing the transfer of a monosaccharide moiety from a NDP-sugar to the 3′ carbon of a sugar moiety of a substrate, such as a terpenoid glycan, thereby functioning as a “1-3 UGT.” In other aspects, the invention provides polynucleotides encoding the 1-3 UGT, and host cells comprising the same. In still other aspects, the invention provides methods for preparing glycosylated substrates, including steviol glycosides, using the enzyme and host cells of this disclosure.
C12P 19/56 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un système cyclique condensé d'au moins trois carbocycles, p. ex. daunomycine, adriamycine
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
71.
Metabolic engineering for microbial production of terpenoid products
The invention relates to methods and bacterial strains for making terpene and terpenoid products, the bacterial strains having improved carbon pull through the MEP pathway and to a downstream recombinant synthesis pathway.
In various aspects, the present invention provides uridine diphosphate-dependent glycosyltransferase (UGT) enzymes capable of catalyzing the transfer of a monosaccharide moiety from a NDP-sugar to the 3' carbon of a sugar moiety of a substrate, such as a terpenoid glycan, thereby functioning as a "1-3 UGT." In other aspects, the invention provides polynucleotides encoding the 1-3 UGT, and host cells comprising the same. In still other aspects, the invention provides methods for preparing glycosylated substrates, including steviol glycosides, using the enzyme and host cells of this disclosure.
In various aspects, the present invention provides uridine diphosphate-dependent glycosyltransferase (UGT) enzymes capable of catalyzing the transfer of a monosaccharide moiety from a NDP-sugar to the 3' carbon of a sugar moiety of a substrate, such as a terpenoid glycan, thereby functioning as a "1-3 UGT." In other aspects, the invention provides polynucleotides encoding the 1-3 UGT, and host cells comprising the same. In still other aspects, the invention provides methods for preparing glycosylated substrates, including steviol glycosides, using the enzyme and host cells of this disclosure.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
30 - Aliments de base, thé, café, pâtisseries et confiseries
Produits et services
Chemical compound used as a sweetening ingredient in the
manufacture of food products and beverages. Dietary aid, namely, a low calorie-sweetening ingredient
used in the manufacture of food products and beverages. Sweetening ingredient used in the manufacture of food
products and beverages.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
30 - Aliments de base, thé, café, pâtisseries et confiseries
Produits et services
Chemical compound used as a sweetening ingredient in the
manufacture of food products and beverages. Dietary aid, namely, a low calorie-sweetening ingredient
used in the manufacture of food products and beverages. Sweetening ingredient used in the manufacture of food
products and beverages; natural sweetener; sugar
substitutes; sweeteners principally comprised of natural
sweetener.
76.
Metabolic engineering for microbial production of terpenoid products
In various aspects and embodiments, the invention relates to bacterial strains and methods for making terpene and terpenoid products. The invention provides bacterial strains with improved carbon flux through the MEP pathway, to thereby increase terpene and/or terpenoid product yield by fermentation with carbon sources such as glucose.
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12P 7/02 - Préparation de composés organiques contenant de l'oxygène contenant un groupe hydroxyle
C12P 17/02 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle
05 - Produits pharmaceutiques, vétérinaires et hygièniques
30 - Aliments de base, thé, café, pâtisseries et confiseries
Produits et services
Dietary aid, namely, a low calorie-sweetening ingredient in the nature of dietetic sugar substitutes for medical use used in the manufacture of food products and beverages Sweetening ingredient in the nature of natural sweeteners and sugar substitutes used in the manufacture of food products and beverages; natural sweetener; sugar substitutes; sweeteners principally comprised of natural sweetener
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
30 - Aliments de base, thé, café, pâtisseries et confiseries
Produits et services
Chemical compound, derived from plants, used as a sweetening ingredient in the manufacture of food products and beverages Sweetening ingredient in the nature of sweeteners used in the manufacture of food products and beverages, none of the foregoing comprising or containing artificial sweeteners
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
Produits et services
Enzymes for scientific and research purposes; enzymes, namely, nucleases, for scientific or research use in connection with cell manipulation, cell transformation, genomic editing and genomic editing analysis; kits for gene editing comprising enzymes for scientific and research purposes
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Providing temporary use of non-downloadable software for designing and editing libraries of genetic cassettes; providing temporary use of non-downloadable software for designing and editing cells, genomes, and genetic cassettes
Escherichia coliYarrowialipolyticalipolytica). The enzymes include a heterologous cannabigerolic acid synthase as well as additional enzymes involved in the biosynthesis of cannabinoid precursors such as geranyl diphosphate, olivetol, olivetolic acid, divarin and/or divarinic acid. Methods are provided for producing C5-cannabinoids and/or C3-cannabinoids by fermentation of the recombinant host cell. Alternatively, cannabinoids can be produced by biotransformation of cannabinoid precursors in recombinant cells or by disrupted recombinant cells.
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
30 - Aliments de base, thé, café, pâtisseries et confiseries
Produits et services
Sweetening ingredient in the nature of a sugar substitute used in the manufacture of food products and beverages; natural sweetener; sugar substitutes; sweeteners principally comprised of natural sweetener; none of the foregoing comprising or containing chemically synthesized sweeteners
84.
BIOSYNTHESIS AND RECOVERY OF SECONDARY METABOLITES
Aspects of the invention provide methods for producing one or more secondary metabolites from microbial culture. In various embodiments, the method comprises culturing a microbial cell producing a secondary metabolite for recovery from a bioreactor medium, the medium comprising an aqueous phase and an extraction phase. The composition of the extraction phase, and the relevant amount with respect to the aqueous phase, enhances production of the secondary metabolite from microbial cells and/or enhances extracellular transfer of the metabolite.
C12N 1/26 - Procédés utilisant des hydrocarbures ou milieux de culture en contenant
C12P 1/04 - Préparation de composés ou de compositions, non prévue dans les groupes , utilisant des micro-organismes ou des enzymesProcédés généraux de préparation de composés ou de compositions utilisant des micro-organismes ou des enzymes utilisant des bactéries
C12P 1/06 - Préparation de composés ou de compositions, non prévue dans les groupes , utilisant des micro-organismes ou des enzymesProcédés généraux de préparation de composés ou de compositions utilisant des micro-organismes ou des enzymes utilisant des actinomycètes
C12P 7/00 - Préparation de composés organiques contenant de l'oxygène
C12P 19/00 - Préparation de composés contenant des radicaux saccharide
C12P 23/00 - Préparation de composés contenant un cycle cyclohexène comportant une chaîne latérale non saturée d'au moins dix atomes de carbone liés par des doubles liaisons conjuguées, p. ex. carotènes
In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced glycosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl transferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells.
C12P 19/18 - Préparation de composés contenant des radicaux saccharide préparés par action d'une transférase glycosylique, p. ex. alpha-, bêta- ou gamma-cyclodextrines
The present disclosure provides methods and compositions for producing rotundone. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of rotundone. In some embodiments, the present disclosure provides microbial host cells for producing rotundone at high purity and/or yield, from either enzymatic transformation of a- guaiene, or from sugar or other carbon source. The present disclosure further provides methods of making products containing rotundone, including flavor or fragrance products, among others.
In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced gly- cosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl trans- ferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells.
C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
C12P 19/00 - Préparation de composés contenant des radicaux saccharide
C12P 19/18 - Préparation de composés contenant des radicaux saccharide préparés par action d'une transférase glycosylique, p. ex. alpha-, bêta- ou gamma-cyclodextrines
C12P 19/44 - Préparation d'O-glucosides, p. ex. glucosides
88.
PRODUCTION OF STEVIOL GLYCOSIDES THROUGH WHOLE CELL BIOTRANSFORMATION
In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced glycosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl transferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells.
C12P 19/56 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un système cyclique condensé d'au moins trois carbocycles, p. ex. daunomycine, adriamycine
C12P 19/44 - Préparation d'O-glucosides, p. ex. glucosides
C12P 19/00 - Préparation de composés contenant des radicaux saccharide
C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression
89.
Metabolic engineering for microbial production of terpenoid products
The invention relates to methods and bacterial strains for making terpene and terpenoid products, the bacterial strains having improved carbon pull through the MEP pathway and to a downstream recombinant synthesis pathway.
The present invention is related to enzymatic pathways for production of carminic acid, host cells capable of production of carminic acid, and methods for the production of carminic acid and related compounds.
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
C07K 1/00 - Procédés généraux de préparation de peptides
C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
C12N 1/15 - ChampignonsLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
Produits et services
Reagents and kits comprising reagents for scientific or research use in connection with cell manipulation, cell transformation, genomic editing and genomic editing analysis Scientific and laboratory apparatus and instruments for manipulating cells and editing genomes
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
09 - Appareils et instruments scientifiques et électriques
Produits et services
Reagents and kits comprising reagents for scientific or research use in connection with cell manipulation, cell transformation, genomic editing and genomic editing analysis Scientific and laboratory apparatus and instruments for manipulating cells and editing genomes
93.
ENZYMES, CELLS, AND METHODS FOR PRODUCTION OF 3-(4-FARNESYLOXYPHENYL)PROPIONIC ACID
The present disclosure provides microbial cells and methods of producing FOPPA resulting from unique biosynthetic pathways, including biosynthetic pathways based on the phenylalanine/tyrosine biosynthetic branch and biosynthetic pathways based on bacteria metabolism. In particular, the present invention provides methods of producing FOPPA in microbial cells. These methods provide a low-cost, sustainable, and environmentally friendly source for FOPPA.
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 1/19 - LevuresLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
A61K 8/97 - Cosmétiques ou préparations similaires pour la toilette caractérisés par la composition contenant des produits de constitution indéterminée ou leurs dérivés à base d’algues, de champignons, de lichens ou de plantesCosmétiques ou préparations similaires pour la toilette caractérisés par la composition contenant des produits de constitution indéterminée ou leurs dérivés à base de leurs dérivés
Siraitiagrosvenoriigrosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog. V, Mog. VI, and Isomog. V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog. V, Mog. VI, and Isomog. V, including food products, beverages, oral care products, sweeteners, and flavoring products.
The invention provides methods for making steviol glycosides, including RebM and glycosylation products that are minor products in stevia leaves, and provides enzymes, encoding polynucleotides, and host cells for use in these methods. The invention provides engineered enzymes and engineered host cells for producing steviol glycosylation products, such as RebM, at high purity and/or yield. The invention further provides methods of making products containing steviol glycosides, such as RebM, including food products, beverages, oral care products, sweeteners, and flavoring products.
C12P 19/56 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un système cyclique condensé d'au moins trois carbocycles, p. ex. daunomycine, adriamycine
E. coliE. coli), that are engineered to overexpress and/or delete or inactivate one or more steviol glycoside transport proteins. The bacterial cells selectively export RebM, or other specific combination of steviol glycosides, out of the cell to increase productivity and reduce production costs associated with downstream purification. Non-target steviol glycosides are not transported to the extracellular medium in significant amounts.
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12P 19/56 - Préparation d'O-glucosides, p. ex. glucosides avec un atome d'oxygène du radical saccharide lié directement à un système cyclique condensé d'au moins trois carbocycles, p. ex. daunomycine, adriamycine
C12N 15/70 - Vecteurs ou systèmes d'expression spécialement adaptés à E. coli
99.
Metabolic engineering for microbial production of terpenoid products
In various aspects and embodiments, the invention relates to bacterial strains and methods for making terpene and terpenoid products. The invention provides bacterial strains with improved carbon flux through the MEP pathway, to thereby increase terpene and/or terpenoid product yield by fermentation with carbon sources such as glucose.
C12N 9/02 - Oxydoréductases (1.), p. ex. luciférase
C12N 9/04 - Oxydoréductases (1.), p. ex. luciférase agissant sur des groupes CHOH comme donneurs, p. ex. oxydase de glucose, déshydrogénase lactique (1.1)
C12P 7/02 - Préparation de composés organiques contenant de l'oxygène contenant un groupe hydroxyle
C12P 17/02 - Préparation de composés hétérocycliques comportant O, N, S, Se ou Te comme uniques hétéro-atomes du cycle l'oxygène comme unique hétéro-atome du cycle
100.
METABOLIC ENGINEERING FOR MICROBIAL PRODUCTION OF TERPENOID PRODUCTS
In various aspects and embodiments, the invention relates to bacterial strains and methods for making terpene and terpenoid products. The invention provides bacterial strains with improved carbon flux through the MEP pathway, to thereby increase terpene and/or terpenoid product yield by fermentation with carbon sources such as glucose.
C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
C12N 9/00 - Enzymes, p. ex. ligases (6.)ProenzymesCompositions les contenantProcédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
C12N 15/52 - Gènes codant pour des enzymes ou des proenzymes
