The present invention relates to purification technology using affinity purification technology. In particular the invention relates to a method for purification of a target product using a dual affinity polypeptide wherein the use of solid matrices or columns are avoided.
A system and a method for filtering a predetermined molecule entrained as a 3D-complex in a liquid comprising a filter forming solid all forming a filter forming suspension, the system having a filter chamber into which the filter forming suspension is fed and drained to form a filter with inclusions of the 3D-complex, where after a cleansing liquid is fed to a neighbouring liquid chamber and fed evenly through the filter to cleanse the filter and subsequently dissolve the complex to retrieve the product or target molecule.
B01D 37/02 - Precoating the filtering elements or materialAddition of filter aids to the liquid being filtered
B01D 29/01 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor with flat filtering elements
A system and a method for filtering a predetermined molecule entrained as a 3D-complex in a liquid comprising a filter forming solid all forming a filter forming suspension, the system having a filter chamber into which the filter forming suspension is fed and drained to form a filter with inclusions of the 3D-complex, where after a cleansing liquid is fed to a neighbouring liquid chamber and fed evenly through the filter to cleanse the filter and subsequently dissolve the complex to retrieve the product or target molecule.
B01D 25/12 - Filter presses, i.e. of the plate or plate and frame type
B01D 29/01 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor with flat filtering elements
B01D 37/02 - Precoating the filtering elements or materialAddition of filter aids to the liquid being filtered
A system and a method for filtering a predetermined molecule entrained as a 3D-complex in a liquid comprising a filter forming solid all forming a filter forming suspension, the system having a filter chamber into which the filter forming suspension is fed and drained to form a filter with inclusions of the 3D-complex, where after a cleansing liquid is fed to a neighbouring liquid chamber and fed evenly through the filter to cleanse the filter and subsequently dissolve the complex to retrieve the product or target molecule.
B01D 25/12 - Filter presses, i.e. of the plate or plate and frame type
B01D 29/01 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor with flat filtering elements
B01D 37/02 - Precoating the filtering elements or materialAddition of filter aids to the liquid being filtered
Ligand functionalized substrate including a solid substrate, which has been modified to provide grafted catching ligand groups covalently bound via a linker, methods of preparing the ligand functionalized substrate and the use thereof, such as to increase binding rate and the dynamic binding capacity (DBC).
SOLVENTUM INTELLECTUAL PROPERTIES COMPANY (Denmark)
CHRETO APS (Denmark)
CHRETO APS (Denmark)
Inventor
Kyhse-Andersen, Jan
Winther, Lars
Rasmussen, Jerald
Abstract
Ligand functionalized substrate including a solid substrate, which has been modified to provide grafted catching ligand groups covalently bound via a linker, methods of preparing said ligand functionalized substrate and the use thereof, such as to increase binding rate and the dynamic binding capacity (DBC).
Ligand functionalized substrate including a solid substrate, which has been modified to provide grafted catching ligand groups covalently bound via a linker, methods of preparing said ligand functionalized substrate and the use thereof, such as to increase binding rate and the dynamic binding capacity (DBC).
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
07 - Machines and machine tools
09 - Scientific and electric apparatus and instruments
37 - Construction and mining; installation and repair services
41 - Education, entertainment, sporting and cultural services
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemicals used in industry, science and medicine; chemicals for the manufacture of pharmaceuticals; Biological preparations for use in industry and science; biological preparations for the manufacture of pharmaceuticals; Diagnostic reagents for scientific use. Pharmaceutical, medical and veterinary products; Sanitary preparations for medical purposes; Dietetic food and substances adapted for medical or veterinary use; Dietary supplements for humans and animals; Diagnostic preparations and materials; Biological preparations for medical purposes. Installations and machines for the production and manufacture of pharmaceutical and biological preparations; Machines and installations for manufacture of diagnostic preparations and materials. Scientific and scientific research apparatus and instruments; apparatus and components for use in quantification; devices for analysing protein sequence and for use as laboratory apparatus. Factory construction; Construction of medical establishments; Installation of production plants; Building consultancy; Consultancy relating to the installation of production plants. Education; Providing of training; Film production services. Scientific and technological services and research and design relating thereto; technical project management in the medical field.
The present invention relates to a process for purification of a target molecule, comprising the steps: (a) contacting a target molecule, and a population of target binding polypeptides (TBP), in solution for a sufficient time to allow complex formation; and (b) isolating the target from the complex from (a) by subsequent purification steps, wherein (i) the target binding polypeptides have at least two binding functionalities; a first binding functionality towards the target and a second binding functionality towards a catching ligand comprised in a solid support; and (ii) the first binding functionality comprises at least two binding sites for the target, and the target comp¬ rises at least two binding sites for the TBP.
The present invention relates to a process for purification of a target molecule, comprising the steps: (a) contacting a target molecule, and a population of target binding polypeptides (TBP), in solution for a sufficient time to allow complex formation;and (b) isolating the target from the complex from (a) by subsequent purification steps, wherein (i) the target binding polypeptides have at least two binding functionalities; a first binding functionality towards the target and a second binding functionalitytowards a catching ligand comprised in a solid support; and (ii) the first binding functionality comprises at least two binding sites for the target, and the target com- prises at least two binding sites for the TBP.
C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
C07K 14/435 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans
The present invention relates to a process for purification of a target biomolecule, comprising the steps: (a) contact-ing (i) a target biomolecule, (ii) a dual affinity polypeptide, and (iii) a solid support comprising a catching ligand, wherein the ratio between the equilibrium dissociation constants of the dual affinity polypeptide, [K D,t / K D,s], is at least 10.degree. at standard conditions; and (b) recovering the target biomolecule by elution.
