The present invention relates to purification technology using affinity purification technology. In particular the invention relates to a method for purification of a target product using a dual affinity polypeptide wherein the use of solid matrices or columns are avoided.
A system and a method for filtering a predetermined molecule entrained as a 3D-complex in a liquid comprising a filter forming solid all forming a filter forming suspension, the system having a filter chamber into which the filter forming suspension is fed and drained to form a filter with inclusions of the 3D-complex, where after a cleansing liquid is fed to a neighbouring liquid chamber and fed evenly through the filter to cleanse the filter and subsequently dissolve the complex to retrieve the product or target molecule.
B01D 37/02 - Revêtement préalable des éléments filtrants ou de la substance filtranteAddition d'adjuvants de filtration au liquide à filtrer
B01D 29/01 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants avec des éléments filtrants plats
A system and a method for filtering a predetermined molecule entrained as a 3D-complex in a liquid comprising a filter forming solid all forming a filter forming suspension, the system having a filter chamber into which the filter forming suspension is fed and drained to form a filter with inclusions of the 3D-complex, where after a cleansing liquid is fed to a neighbouring liquid chamber and fed evenly through the filter to cleanse the filter and subsequently dissolve the complex to retrieve the product or target molecule.
B01D 25/12 - Filtres-presses, c.-à-d. du type à plaque ou du type à plaque et cadre
B01D 29/01 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants avec des éléments filtrants plats
B01D 37/02 - Revêtement préalable des éléments filtrants ou de la substance filtranteAddition d'adjuvants de filtration au liquide à filtrer
A system and a method for filtering a predetermined molecule entrained as a 3D-complex in a liquid comprising a filter forming solid all forming a filter forming suspension, the system having a filter chamber into which the filter forming suspension is fed and drained to form a filter with inclusions of the 3D-complex, where after a cleansing liquid is fed to a neighbouring liquid chamber and fed evenly through the filter to cleanse the filter and subsequently dissolve the complex to retrieve the product or target molecule.
B01D 25/12 - Filtres-presses, c.-à-d. du type à plaque ou du type à plaque et cadre
B01D 29/01 - Filtres à éléments filtrants stationnaires pendant la filtration, p. ex. filtres à aspiration ou à pression, non couverts par les groupes Leurs éléments filtrants avec des éléments filtrants plats
B01D 37/02 - Revêtement préalable des éléments filtrants ou de la substance filtranteAddition d'adjuvants de filtration au liquide à filtrer
Ligand functionalized substrate including a solid substrate, which has been modified to provide grafted catching ligand groups covalently bound via a linker, methods of preparing the ligand functionalized substrate and the use thereof, such as to increase binding rate and the dynamic binding capacity (DBC).
SOLVENTUM INTELLECTUAL PROPERTIES COMPANY (Danemark)
CHRETO APS (Danemark)
Inventeur(s)
Kyhse-Andersen, Jan
Winther, Lars
Rasmussen, Jerald
Abrégé
Ligand functionalized substrate including a solid substrate, which has been modified to provide grafted catching ligand groups covalently bound via a linker, methods of preparing said ligand functionalized substrate and the use thereof, such as to increase binding rate and the dynamic binding capacity (DBC).
Ligand functionalized substrate including a solid substrate, which has been modified to provide grafted catching ligand groups covalently bound via a linker, methods of preparing said ligand functionalized substrate and the use thereof, such as to increase binding rate and the dynamic binding capacity (DBC).
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
05 - Produits pharmaceutiques, vétérinaires et hygièniques
07 - Machines et machines-outils
09 - Appareils et instruments scientifiques et électriques
37 - Services de construction; extraction minière; installation et réparation
41 - Éducation, divertissements, activités sportives et culturelles
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Chemicals used in industry, science and medicine; chemicals for the manufacture of pharmaceuticals; Biological preparations for use in industry and science; biological preparations for the manufacture of pharmaceuticals; Diagnostic reagents for scientific use. Pharmaceutical, medical and veterinary products; Sanitary preparations for medical purposes; Dietetic food and substances adapted for medical or veterinary use; Dietary supplements for humans and animals; Diagnostic preparations and materials; Biological preparations for medical purposes. Installations and machines for the production and manufacture of pharmaceutical and biological preparations; Machines and installations for manufacture of diagnostic preparations and materials. Scientific and scientific research apparatus and instruments; apparatus and components for use in quantification; devices for analysing protein sequence and for use as laboratory apparatus. Factory construction; Construction of medical establishments; Installation of production plants; Building consultancy; Consultancy relating to the installation of production plants. Education; Providing of training; Film production services. Scientific and technological services and research and design relating thereto; technical project management in the medical field.
The present invention relates to a process for purification of a target molecule, comprising the steps: (a) contacting a target molecule, and a population of target binding polypeptides (TBP), in solution for a sufficient time to allow complex formation; and (b) isolating the target from the complex from (a) by subsequent purification steps, wherein (i) the target binding polypeptides have at least two binding functionalities; a first binding functionality towards the target and a second binding functionality towards a catching ligand comprised in a solid support; and (ii) the first binding functionality comprises at least two binding sites for the target, and the target comp¬ rises at least two binding sites for the TBP.
The present invention relates to a process for purification of a target molecule, comprising the steps: (a) contacting a target molecule, and a population of target binding polypeptides (TBP), in solution for a sufficient time to allow complex formation;and (b) isolating the target from the complex from (a) by subsequent purification steps, wherein (i) the target binding polypeptides have at least two binding functionalities; a first binding functionality towards the target and a second binding functionalitytowards a catching ligand comprised in a solid support; and (ii) the first binding functionality comprises at least two binding sites for the target, and the target com- prises at least two binding sites for the TBP.
C07K 1/22 - Chromatographie d'affinité ou techniques analogues basées sur des procédés d'absorption sélective
C07K 14/435 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'animauxPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'humains
The present invention relates to a process for purification of a target biomolecule, comprising the steps: (a) contact-ing (i) a target biomolecule, (ii) a dual affinity polypeptide, and (iii) a solid support comprising a catching ligand, wherein the ratio between the equilibrium dissociation constants of the dual affinity polypeptide, [K D,t / K D,s], is at least 10.degree. at standard conditions; and (b) recovering the target biomolecule by elution.
