Embodiments of the present disclosure are directed to ferrofluid¬ based assay methods, systems and device for detecting one or more parasite oocyst or egg, and more specifically, to detecting parasite oocysts or eggs in fecal matter of livestock, so as to determine parasitic infections within such livestock. Such embodiments are configured to help in the ability to maintain healthy livestock for human consumption.
G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
G01N 27/74 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables of fluids
G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor
Embodiments of the present disclosure include systems, devices and methods for increasing the accuracy of an MPN, using assay instrumentation. For example, such embodiments can be accomplished by pre-loading the assays system with standard curves generated from measurements made with dilutions of known levels of pathogens. When such an approach is used, for each sample, the value from the last positive dilution and a calibrated assay count can both be used to provide a more accurate CFU per sample value than would be determined from just the last positive dilution alone.
The subject disclosure presents, in some embodiments, is systems, devices and methods for multi-sample analyte analysis. In some such embodiments, a method is presented which includes labeling at least one target analyte in each of a plurality of individual samples with at least one unique label probe. The method further includes combining the plurality of individual samples into a mixture, and flowing the mixture within at least one fluidic channel/1 ane having arranged thereon or in fluid communication with at least one set of a plurality of spatially distinct capture zones. Embodiments of the present disclosure additionally relate to, among other things, systems, devices, and related methods for operating the same, to analyze and rapidly identify and quantify microbial pathogens in environmental, food, and clinical settings. Specifically, a rapid one-step FISH method where fixation, permeabilization and addition of fluorescent labeled probe all occur in one step on a ferrofluidic system.
Embodiments herein include a scanning apparatus for detecting target particles present within a ferrofluid, where the scanning apparatus can be used in a microfluidic system. The methods and structures described herein also include, for example, a scanning device comprising an optical system, a first magnet disposed on a first side of the optical system, where the first magnet can be non-rotatable, a second magnet disposed on a second side of the optical system, opposite the first magnet, where the second magnet can be rotatable, and a lever arm coupled to the second magnet, where the lever is capable of rotating the second magnet.
B03C 1/033 - Component partsAuxiliary operations characterised by the magnetic circuit
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
B03C 1/20 - Magnetic separation acting directly on the substance being separated with material carriers in the form of belts with magnets moving during operation in the form of belts, e.g. cross-belt type
Embodiments of the present disclosure are directed to methods of forming a concentrated ferrofluid, and methods of using the concentrated ferrofluid. In some embodiments, these methods include filtering a ferrofluid comprising a plurality of magnetic nanoparticles, a surfactant and a continuous phase, where the ferrofluidic comprises a first density. The ferrofluidic is dried, where the magnetic nanoparticles and the surfactant remain, and where at least a portion of the continuous phase is removed. A slurry may be formed comprising the dried ferrofluid and the additional amount of the continuous phase. A colloidal suspension comprising a second density of the ferrofluid can be formed by ball milling.
H01F 1/44 - Magnets or magnetic bodies characterised by the magnetic materials thereforSelection of materials for their magnetic properties of magnetic liquids, e.g. ferrofluids
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