Abrégé

Embodiments of the present disclosure are directed to ferrofluid¬ based assay methods, systems and device for detecting one or more parasite oocyst or egg, and more specifically, to detecting parasite oocysts or eggs in fecal matter of livestock, so as to determine parasitic infections within such livestock. Such embodiments are configured to help in the ability to maintain healthy livestock for human consumption.

Classes IPC  ?

• G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
• G01N 27/74 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant des variables magnétiques des fluides
• G01N 33/569 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour micro-organismes, p. ex. protozoaires, bactéries, virus
• G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
• G06T 7/00 - Analyse d'image

Abrégé

Embodiments of the present disclosure include systems, devices and methods for increasing the accuracy of an MPN, using assay instrumentation. For example, such embodiments can be accomplished by pre-loading the assays system with standard curves generated from measurements made with dilutions of known levels of pathogens. When such an approach is used, for each sample, the value from the last positive dilution and a calibrated assay count can both be used to provide a more accurate CFU per sample value than would be determined from just the last positive dilution alone.

Classes IPC  ?

• C12Q 1/06 - Détermination quantitative
• C40B 70/00 - Étiquettes ["tags"] ou marqueurs ["labels"] spécialement adaptés à la chimie combinatoire ou aux chimiothèques, p. ex. "tags" fluorescents ou codes-barres

Abrégé

The subject disclosure presents, in some embodiments, is systems, devices and methods for multi-sample analyte analysis. In some such embodiments, a method is presented which includes labeling at least one target analyte in each of a plurality of individual samples with at least one unique label probe. The method further includes combining the plurality of individual samples into a mixture, and flowing the mixture within at least one fluidic channel/1 ane having arranged thereon or in fluid communication with at least one set of a plurality of spatially distinct capture zones. Embodiments of the present disclosure additionally relate to, among other things, systems, devices, and related methods for operating the same, to analyze and rapidly identify and quantify microbial pathogens in environmental, food, and clinical settings. Specifically, a rapid one-step FISH method where fixation, permeabilization and addition of fluorescent labeled probe all occur in one step on a ferrofluidic system.

Classes IPC  ?

• C12Q 1/6804 - Analyse d’acides nucléiques utilisant des immunogènes
• C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
• G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides

Abrégé

Embodiments herein include a scanning apparatus for detecting target particles present within a ferrofluid, where the scanning apparatus can be used in a microfluidic system. The methods and structures described herein also include, for example, a scanning device comprising an optical system, a first magnet disposed on a first side of the optical system, where the first magnet can be non-rotatable, a second magnet disposed on a second side of the optical system, opposite the first magnet, where the second magnet can be rotatable, and a lever arm coupled to the second magnet, where the lever is capable of rotating the second magnet.

Classes IPC  ?

• B03C 1/033 - Éléments constitutifsOpérations auxiliaires caractérisés par le circuit magnétique
• B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
• B03C 1/20 - Séparation magnétique agissant directement sur la substance à séparer ayant des supports pour le matériau traité en forme de bandes avec des aimants se déplaçant pendant l'opération en forme de bandes, p. ex. du type à bande transversale
• G01N 21/64 - FluorescencePhosphorescence
• G01N 21/76 - ChimioluminescenceBioluminescence
• G01N 33/553 - Support métallique ou recouvert d'un métal

Abrégé

Embodiments of the present disclosure are directed to methods of forming a concentrated ferrofluid, and methods of using the concentrated ferrofluid. In some embodiments, these methods include filtering a ferrofluid comprising a plurality of magnetic nanoparticles, a surfactant and a continuous phase, where the ferrofluidic comprises a first density. The ferrofluidic is dried, where the magnetic nanoparticles and the surfactant remain, and where at least a portion of the continuous phase is removed. A slurry may be formed comprising the dried ferrofluid and the additional amount of the continuous phase. A colloidal suspension comprising a second density of the ferrofluid can be formed by ball milling.

Classes IPC  ?

• H01F 1/44 - Aimants ou corps magnétiques, caractérisés par les matériaux magnétiques appropriésEmploi de matériaux spécifiés pour leurs propriétés magnétiques en liquides magnétiques, p. ex. ferrofluides
• B03C 1/00 - Séparation magnétique
• B03C 1/005 - Prétraitement spécialement adapté à la séparation magnétique
• B03C 1/02 - Séparation magnétique agissant directement sur la substance à séparer
• H01F 1/00 - Aimants ou corps magnétiques, caractérisés par les matériaux magnétiques appropriésEmploi de matériaux spécifiés pour leurs propriétés magnétiques