Abstract

Disclosed are use of a polynucleotide, protein, and biological material in the regulation and control of plant tuber development, and related products and production methods thereof. The tuber plant or part thereof, comprising a sequence encoding a polypeptide comprising a sequence having at least 92% sequence identity to SEQ ID NO: 2, wherein the polypeptide is inhibited-expressed, loss-function, or overexpressed, and the development of plant tuber is changed. The polynucleotide is an identity gene of a tuber, and can control whether a tuber is formed, the formation time, the tuber size etc. by regulating and controlling a protein function, an expression quantity etc.

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• A01H 6/82 - Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
• C12N 9/22 - Ribonucleases

Abstract

A plant leaf sampler includes a sampling portion, a transfer portion and a storage portion, wherein: the sampling portion comprises a sampling punch, a movable sampling plate, a collecting plate and a docking chamber, the collecting plate is provided with a through-hole capable of allowing at least a part of a tip of the sampling punch to enter, the docking chamber is located below the collecting plate and is provided such that the movable sampling plate enters and exits the docking chamber; the transfer portion includes a track for guiding movement of the sampling plate, an in-cassette punch assembly for dropping the sample from the sampling plate and an anchor assembly for combining the sampling plate with the in-cassette punch assembly; and the storage portion comprises a storage cassette provided with at least one storage hole, the storage cassette being detachably mounted to a bottom of the plant leaf sampler.

IPC Classes  ?

• G01N 1/04 - Devices for withdrawing samples in the solid state, e.g. by cutting
• G01N 33/00 - Investigating or analysing materials by specific methods not covered by groups

Abstract

The present invention belongs to the technical field of biology, and provides a polynucleotide, a protein, a biological material, and use thereof in improving the quality of a plant fruit. The polynucleotide comprises a sequence set forth in any one of SEQ ID NOs: 3, 11, 23-36, and 95-108; a complementary sequence, a degenerate sequence, or a homologous sequence of the described sequence; a sequence hybridized with the described sequence or a complementary sequence thereof under rigorous conditions; and a cDNA sequence of the described sequence. It is found in the research that the gene is a key regulatory gene affecting the sugar content of the fruit, and in the use, the content of SSC, glucose, fructose, citric acid, and malic acid of the plant fruit can be regulated by regulating the protein function, the expression level, and the like of the gene without significantly affecting the weight of the fruit, such that the quality and flavor of a tomato fruit are improved.

IPC Classes  ?

• C12N 15/54 - Transferases (2)
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• A01H 6/82 - Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant

Abstract

The present invention provides a PCR marker for detecting an exogenous radish fragment in a Brassica oleracea-radish introgression line, comprising one or more of PCR markers BoRa1-BoRa26. The nucleotide sequences of the BoRa1-BoRa26 are as shown in in SEQ ID NOs: 1-26, respectively. Also provided is a primer set for amplifying the PCR marker, comprising one or more pairs of primer pairs BoRa1p-BoRa26p.

IPC Classes  ?

• C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Abstract

Disclosed in the present invention is a plant leaf sampler. The plant leaf sampler comprises a sampling part, a transfer part and a storage part. The sampling part comprises a sampling punch, a movable sampling plate, a collecting plate and a parking bin. The collecting plate is provided with a through hole capable of allowing at least part of the tail end of the sampling punch to pass through, and the parking bin is located under the collecting plate and is configured to enable the movable sampling plate to enter and exit the parking bin. The transfer part comprises a rail used for guiding the sampling plate to move, a case-entering punch assembly used for enabling a sample to fall from the sampling plate and an anchoring assembly used for combining the sampling plate and the case-entering punch assembly. The storage part comprises a storage case provided with at least one storage hole, the storage case being detachably installed at the bottom of the plant leaf sampler. The instrument of the present invention can quickly and efficiently collect a large number of plant samples and therefore is specially suitable for high-throughput extraction of plant DNA.

IPC Classes  ?

• G01N 1/04 - Devices for withdrawing samples in the solid state, e.g. by cutting

Abstract

12122 is a sequence set forth in SEQ ID NO: 2; a complementary sequence, a degenerate sequence or a homologous sequence thereof; a nucleotide sequence hybridized therewith or a complementary sequence thereof; and a cDNA sequence of a sequence described above. By means of utilizing a gene editing tool, a conserved B-region fragment of a YABBY1 gene 5'UTR of the cucurbitaceous plant or a partial fragment thereof is deleted, such that the translation efficiency of YABBY1 is improved, thereby shortening the length of the main stem of the cucurbitaceous plant, achieving the purposes of plant type dwarfing and high-yield close planting of the cucurbitaceous plant, and meanwhile, allowing for significant labor savings.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/84 - Ti-plasmids
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• A01H 5/04 - Stems
• A01H 5/10 - Seeds
• A01H 6/34 - Cucurbitaceae, e.g. bitter melon, cucumber or watermelon

Abstract

The present application relates to the field of plant genetic engineering, in particular to an application of a polynucleotide, protein and biological material in the regulation and control of plant tuber development, and related products and cultivation methods therefor. The polynucleotide is a nucleotide sequence as shown in SEQ ID NO: 1; or a complementary sequence, degenerate sequence, or homologous sequence thereof; or a polynucleotide or complementary sequence thereof hybridized with the nucleotide sequence as shown in SEQ ID NO: 1 under a tight condition; or a cDNA sequence of any one of the above sequences. The polynucleotide is an identity gene of a tuber, and can control whether a tuber is formed, the formation time, the tuber size etc. by regulating and controlling a protein function, an expression quantity etc.

IPC Classes  ?

• C12N 15/29 - Genes encoding plant proteins, e.g. thaumatin
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• A01H 6/82 - Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• A01H 1/02 - Methods or apparatus for hybridisationArtificial pollination
• A01H 4/00 - Plant reproduction by tissue culture techniques
• A23L 19/12 - Products from fruits or vegetablesPreparation or treatment thereof of tuberous or like starch containing root crops of potatoes

Abstract

The present application provides a system and method for performing precise irrigation on the basis of a plant wilting degree, comprising: an image acquisition module, which acquires image information of a plant by means of an image sensor, the acquired information comprising: leaf information and stem information; an image preprocessing module, which is used for completing preprocessing of the image, comprising image cropping, enhancement, color space conversion processing; a water demand prediction module, which is used for inputting the acquired image information into a trained deep neural network for calculation and predicting a predicted water demand corresponding to a current plant wilting degree state; and an irrigation control module, which is used for forming a control instruction according to the predicted water demand of the plant so as to supply water precisely and quantitatively. According to the present invention, the water shortage amount can be accurately estimated only by identifying leaf and stem features, and the model training speed and the prediction precision can be improved by using a loss function, a pooling method, and an excitation function.

IPC Classes  ?

• A01G 25/16 - Control of watering

Abstract

The present application relates to an intelligent moisture precise irrigation control system and method for fruit and vegetable cultivation in a solar greenhouse. The system comprises: an information acquisition module for acquiring information by means of a plurality of sensors, the acquired information comprising at least one of soil information, ground environment information, irrigation device use process information, and crop information; a water demand prediction module for inputting the acquired information into a deep neural network for calculation to predict a water demand of fruits and vegetables currently cultivated in the solar greenhouse; and an irrigation control module for forming a control instruction according to the predicted water demand of the vegetables, so as to supply water periodically and quantitatively. In this way, the irrigation time and the irrigation amount can be decided according to growth conditions and environmental parameters of the vegetables, manual operation is not needed in the whole process, facility cultivation and intelligent irrigation can be achieved, the working efficiency is high, the accuracy is high, and the water-saving, quality-improved and efficiency-improved production of the vegetables is achieved.

IPC Classes  ?

• A01G 9/24 - Devices for heating, ventilating, regulating temperature, or watering, in greenhouses, forcing-frames, or the like

Abstract

Disclosed are activity evaluation of plant pathogens and a high throughput screening method for microbicides based on double fluorescent staining and a kit therefor, which include using a microtiter plate as tool carrier, using two different fluorescent dyes, respectively fluorescently labelling spores or mycelia of plant pathogens having vitality and no vitality, and due to the different colouring of spores or mycelia having no vitality and vitality, quantitatively detecting the survival rate of plant pathogens through fluorescence microscopy or flow cytometry, and accordingly preparing the kit. The method and kit can be used in aspects such as microbicide screening, evaluation of food safety, and water quality evaluation using the plant pathogen survival rate as an indicator. The method and kit are rapid, objective, simple and easy in operation, and have a low cost, and both can be used in studying the mechanism of the action of microbicides and high-throughput screening and the toxicity evaluation of microbicides.

IPC Classes  ?

• G01N 21/64 - FluorescencePhosphorescence

Abstract

Provided are an insect-resistance inducing effect and use of a benzothiadiazole derivative. Specifically, provided is a use of a formula A compound in the preparation of an insect-resistant composition, wherein the groups in the formula are as defined in the specification. Also provided are an insect-resistance inducing composition containing the formula A compound, and an insect-resistance inducing method utilizing the formula A compound or the composition. The method, compound or composition of the present invention induce the insect-resistance of a crop to main insect pests, reduce or mitigate the threat from insect pests, and can also reduce chemical pesticide dosage, and reduce ecological pressure and economic loss, thus having huge market value, application prospect and economic benefit.

IPC Classes  ?

• A01N 43/828 - 1,2,3-Oxa(thia)diazolesHydrogenated 1,2,3-oxa(thia)diazoles
• C07D 417/12 - Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group containing two hetero rings linked by a chain containing hetero atoms as chain links
• A01N 51/00 - Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
• A01P 7/00 - Arthropodicides

Abstract

Application of an elicitor S-methyl benzo-1,2,3-thiadiazole-7-carbothioate and/or trifluoroethyl benzo-1,2,3-thiadiazole-7-carboxylate in control of soil-born diseases of garden crops is provided, including diseases caused by Fusarium oxysporum, Pythium aphanidermatum, Rhizoctonia solani, Sclerotinia sclerotiorum etc.. The elicitor is applied by singly spraying or immersing seed combined with spraying. The elicitor has significant disease resistance-inducing effect and long action.

IPC Classes  ?

• A01N 43/828 - 1,2,3-Oxa(thia)diazolesHydrogenated 1,2,3-oxa(thia)diazoles
• A01P 3/00 - Fungicides