Abrégé

Disclosed are use of a polynucleotide, protein, and biological material in the regulation and control of plant tuber development, and related products and production methods thereof. The tuber plant or part thereof, comprising a sequence encoding a polypeptide comprising a sequence having at least 92% sequence identity to SEQ ID NO: 2, wherein the polypeptide is inhibited-expressed, loss-function, or overexpressed, and the development of plant tuber is changed. The polynucleotide is an identity gene of a tuber, and can control whether a tuber is formed, the formation time, the tuber size etc. by regulating and controlling a protein function, an expression quantity etc.

Classes IPC  ?

• C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
• A01H 6/82 - Solanaceae, p. ex. poivron, tabac, pomme de terre, tomate ou aubergine
• C12N 9/22 - Ribonucléases

Abrégé

A plant leaf sampler includes a sampling portion, a transfer portion and a storage portion, wherein: the sampling portion comprises a sampling punch, a movable sampling plate, a collecting plate and a docking chamber, the collecting plate is provided with a through-hole capable of allowing at least a part of a tip of the sampling punch to enter, the docking chamber is located below the collecting plate and is provided such that the movable sampling plate enters and exits the docking chamber; the transfer portion includes a track for guiding movement of the sampling plate, an in-cassette punch assembly for dropping the sample from the sampling plate and an anchor assembly for combining the sampling plate with the in-cassette punch assembly; and the storage portion comprises a storage cassette provided with at least one storage hole, the storage cassette being detachably mounted to a bottom of the plant leaf sampler.

Classes IPC  ?

• G01N 1/04 - Dispositifs pour prélever des échantillons à l'état solide, p. ex. par coupe à l'outil
• G01N 33/00 - Recherche ou analyse des matériaux par des méthodes spécifiques non couvertes par les groupes

Abrégé

The present invention belongs to the technical field of biology, and provides a polynucleotide, a protein, a biological material, and use thereof in improving the quality of a plant fruit. The polynucleotide comprises a sequence set forth in any one of SEQ ID NOs: 3, 11, 23-36, and 95-108; a complementary sequence, a degenerate sequence, or a homologous sequence of the described sequence; a sequence hybridized with the described sequence or a complementary sequence thereof under rigorous conditions; and a cDNA sequence of the described sequence. It is found in the research that the gene is a key regulatory gene affecting the sugar content of the fruit, and in the use, the content of SSC, glucose, fructose, citric acid, and malic acid of the plant fruit can be regulated by regulating the protein function, the expression level, and the like of the gene without significantly affecting the weight of the fruit, such that the quality and flavor of a tomato fruit are improved.

Classes IPC  ?

• C12N 15/54 - Transférases (2)
• C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p. ex. kinases (2.7)
• A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
• A01H 6/82 - Solanaceae, p. ex. poivron, tabac, pomme de terre, tomate ou aubergine

Abrégé

The present invention provides a PCR marker for detecting an exogenous radish fragment in a Brassica oleracea-radish introgression line, comprising one or more of PCR markers BoRa1-BoRa26. The nucleotide sequences of the BoRa1-BoRa26 are as shown in in SEQ ID NOs: 1-26, respectively. Also provided is a primer set for amplifying the PCR marker, comprising one or more pairs of primer pairs BoRa1p-BoRa26p.

Classes IPC  ?

• C12Q 1/6895 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les plantes, les champignons ou les algues

Abrégé

Disclosed in the present invention is a plant leaf sampler. The plant leaf sampler comprises a sampling part, a transfer part and a storage part. The sampling part comprises a sampling punch, a movable sampling plate, a collecting plate and a parking bin. The collecting plate is provided with a through hole capable of allowing at least part of the tail end of the sampling punch to pass through, and the parking bin is located under the collecting plate and is configured to enable the movable sampling plate to enter and exit the parking bin. The transfer part comprises a rail used for guiding the sampling plate to move, a case-entering punch assembly used for enabling a sample to fall from the sampling plate and an anchoring assembly used for combining the sampling plate and the case-entering punch assembly. The storage part comprises a storage case provided with at least one storage hole, the storage case being detachably installed at the bottom of the plant leaf sampler. The instrument of the present invention can quickly and efficiently collect a large number of plant samples and therefore is specially suitable for high-throughput extraction of plant DNA.

Classes IPC  ?

• G01N 1/04 - Dispositifs pour prélever des échantillons à l'état solide, p. ex. par coupe à l'outil

Abrégé

12122 is a sequence set forth in SEQ ID NO: 2; a complementary sequence, a degenerate sequence or a homologous sequence thereof; a nucleotide sequence hybridized therewith or a complementary sequence thereof; and a cDNA sequence of a sequence described above. By means of utilizing a gene editing tool, a conserved B-region fragment of a YABBY1 gene 5'UTR of the cucurbitaceous plant or a partial fragment thereof is deleted, such that the translation efficiency of YABBY1 is improved, thereby shortening the length of the main stem of the cucurbitaceous plant, achieving the purposes of plant type dwarfing and high-yield close planting of the cucurbitaceous plant, and meanwhile, allowing for significant labor savings.

Classes IPC  ?

• C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
• C12N 15/84 - Plasmides Ti
• C12N 1/21 - BactériesLeurs milieux de culture modifiés par l'introduction de matériel génétique étranger
• A01H 5/04 - Tiges
• A01H 5/10 - Graines
• A01H 6/34 - Cucurbitaceae, p. ex. melon amer, concombre ou pastèque

Abrégé

The present application relates to the field of plant genetic engineering, in particular to an application of a polynucleotide, protein and biological material in the regulation and control of plant tuber development, and related products and cultivation methods therefor. The polynucleotide is a nucleotide sequence as shown in SEQ ID NO: 1; or a complementary sequence, degenerate sequence, or homologous sequence thereof; or a polynucleotide or complementary sequence thereof hybridized with the nucleotide sequence as shown in SEQ ID NO: 1 under a tight condition; or a cDNA sequence of any one of the above sequences. The polynucleotide is an identity gene of a tuber, and can control whether a tuber is formed, the formation time, the tuber size etc. by regulating and controlling a protein function, an expression quantity etc.

Classes IPC  ?

• C12N 15/29 - Gènes codant pour des protéines végétales, p. ex. thaumatine
• C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
• C07K 14/415 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant de végétaux
• C12N 5/10 - Cellules modifiées par l'introduction de matériel génétique étranger, p. ex. cellules transformées par des virus
• A01H 6/82 - Solanaceae, p. ex. poivron, tabac, pomme de terre, tomate ou aubergine
• A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
• A01H 1/02 - Méthodes ou appareils d'hybridationPollinisation artificielle
• A01H 4/00 - Reproduction de plantes par des techniques de culture de tissus
• A23L 19/12 - Produits à base de fruits ou de légumesLeur préparation ou leur traitement de tubercules ou d'autres racines contenant de l'amidon de pommes de terre

Abrégé

The present application provides a system and method for performing precise irrigation on the basis of a plant wilting degree, comprising: an image acquisition module, which acquires image information of a plant by means of an image sensor, the acquired information comprising: leaf information and stem information; an image preprocessing module, which is used for completing preprocessing of the image, comprising image cropping, enhancement, color space conversion processing; a water demand prediction module, which is used for inputting the acquired image information into a trained deep neural network for calculation and predicting a predicted water demand corresponding to a current plant wilting degree state; and an irrigation control module, which is used for forming a control instruction according to the predicted water demand of the plant so as to supply water precisely and quantitatively. According to the present invention, the water shortage amount can be accurately estimated only by identifying leaf and stem features, and the model training speed and the prediction precision can be improved by using a loss function, a pooling method, and an excitation function.

Classes IPC  ?

• A01G 25/16 - Commande de l'arrosage

Abrégé

The present application relates to an intelligent moisture precise irrigation control system and method for fruit and vegetable cultivation in a solar greenhouse. The system comprises: an information acquisition module for acquiring information by means of a plurality of sensors, the acquired information comprising at least one of soil information, ground environment information, irrigation device use process information, and crop information; a water demand prediction module for inputting the acquired information into a deep neural network for calculation to predict a water demand of fruits and vegetables currently cultivated in the solar greenhouse; and an irrigation control module for forming a control instruction according to the predicted water demand of the vegetables, so as to supply water periodically and quantitatively. In this way, the irrigation time and the irrigation amount can be decided according to growth conditions and environmental parameters of the vegetables, manual operation is not needed in the whole process, facility cultivation and intelligent irrigation can be achieved, the working efficiency is high, the accuracy is high, and the water-saving, quality-improved and efficiency-improved production of the vegetables is achieved.

Classes IPC  ?

• A01G 9/24 - Dispositifs de chauffage, d'aération, de régulation de la température ou d'irrigation dans les serres, les châssis ou les installations similaires

Abrégé

Disclosed are activity evaluation of plant pathogens and a high throughput screening method for microbicides based on double fluorescent staining and a kit therefor, which include using a microtiter plate as tool carrier, using two different fluorescent dyes, respectively fluorescently labelling spores or mycelia of plant pathogens having vitality and no vitality, and due to the different colouring of spores or mycelia having no vitality and vitality, quantitatively detecting the survival rate of plant pathogens through fluorescence microscopy or flow cytometry, and accordingly preparing the kit. The method and kit can be used in aspects such as microbicide screening, evaluation of food safety, and water quality evaluation using the plant pathogen survival rate as an indicator. The method and kit are rapid, objective, simple and easy in operation, and have a low cost, and both can be used in studying the mechanism of the action of microbicides and high-throughput screening and the toxicity evaluation of microbicides.

Classes IPC  ?

• G01N 21/64 - FluorescencePhosphorescence

Abrégé

Provided are an insect-resistance inducing effect and use of a benzothiadiazole derivative. Specifically, provided is a use of a formula A compound in the preparation of an insect-resistant composition, wherein the groups in the formula are as defined in the specification. Also provided are an insect-resistance inducing composition containing the formula A compound, and an insect-resistance inducing method utilizing the formula A compound or the composition. The method, compound or composition of the present invention induce the insect-resistance of a crop to main insect pests, reduce or mitigate the threat from insect pests, and can also reduce chemical pesticide dosage, and reduce ecological pressure and economic loss, thus having huge market value, application prospect and economic benefit.

Classes IPC  ?

• A01N 43/828 - Oxa(thia)diazoles-1,2,3Oxa(thia)diazoles-1,2,3 hydrogénés
• C07D 417/12 - Composés hétérocycliques contenant plusieurs hétérocycles, au moins un cycle comportant des atomes de soufre et d'azote comme uniques hétéro-atomes du cycle, non prévus par le groupe contenant deux hétérocycles liés par une chaîne contenant des hétéro-atomes comme chaînons
• A01N 51/00 - Biocides, produits repoussant ou attirant les animaux nuisibles, ou régulateurs de croissance des végétaux, contenant des composés organiques comportant les séquences d'atomes O—N—S, X—O—S, N—N—S, O—N—N ou O-halogène, sans considération du nombre de liaisons de chaque atome, aucun atome de ces séquences ne faisant partie d'un hétérocycle
• A01P 7/00 - Arthropodicides

Abrégé

Application of an elicitor S-methyl benzo-1,2,3-thiadiazole-7-carbothioate and/or trifluoroethyl benzo-1,2,3-thiadiazole-7-carboxylate in control of soil-born diseases of garden crops is provided, including diseases caused by Fusarium oxysporum, Pythium aphanidermatum, Rhizoctonia solani, Sclerotinia sclerotiorum etc.. The elicitor is applied by singly spraying or immersing seed combined with spraying. The elicitor has significant disease resistance-inducing effect and long action.

Classes IPC  ?

• A01N 43/828 - Oxa(thia)diazoles-1,2,3Oxa(thia)diazoles-1,2,3 hydrogénés
• A01P 3/00 - Fongicides