Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in a cerebellar cell type and a secondary cell type.
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in excitatory cortical neurons, such as primarily within cortical layers 2/3, 4, 5, and 6 and including those with extratelencephalic (ET) projections, intratelencephalic (IT) projections, and pyramidal tract (PT) projections, among others.
Seattle Children's Hospital d/b/a Seattle Children's Research Institute (USA)
Inventor
Ting, Jonathan
Levi, Boaz P.
Mich, John K.
Lein, Edward Sebastian
Kalume, Franck
Abstract
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in GABAergic interneurons.
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in serotonergic neurons within the dorsal raphe nucleus and/or the median raphe nucleus.
A computer implemented method of auditing a data analysis process is provided. The method includes generating a certificate of reproducibility that permits a user or auditor to exactly duplicate the various steps involved in the data analysis process, including data ingestion, data processing, and data output. The certificate of reproducibility may include a directed reproducibility graph of events that contribute to the output. The certificate of reproducibility and directed reproducibility graph may be published.
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in dopaminergic neurons of the central nervous system. Wherein, an artificial expression construct comprising (i) an enhancer is eHGT 888m.
THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATE (USA)
BOARD OF REGENTS OF THE UNIVERSITY OF NEBRASKA (USA)
UNIFORMED SERVICES UNIVERSITY OF THE HEALTH SCIENCES (USA)
Inventor
Gillespie, Mark, Andrew
Li, Xiaojun
Savage, Adam, Kenneth
Zaim, Samir, Rachid
Deane, Kevin, Dale
Holers, Vernon, Michael
Mikuls, Ted, Richard
Edison, Jess, David
Abstract
Biomarkers to predict progression from at-risk of developing rheumatoid arthritis (pre-RA) to physician-confirmed rheumatoid arthritis (i.e., Clinical RA) within defined time periods are described. The described biomarkers can be used to identify interventions to prevent, delay, or modulate future Clinical RA and to identify potential stage-specific targets for preventive interventions.
G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease
G16B 25/10 - Gene or protein expression profilingExpression-ratio estimation or normalisation
8.
ARTIFICIAL EXPRESSION CONSTRUCTS FOR MODULATING GENE EXPRESSION IN NEURONS AND ASTROCYTES FOR THE ASSESSMENT AND/OR TREATMENT OF SLC6A1-RELATED DISORDERS
Artificial expression constructs for modulating gene expression in neurons and astrocytes are described. Particular artificial expression constructs modulate gene expression in pan-brain GABAergic neurons and forebrain GABAergic neurons. The artificial expression constructs can be used in the assessment and/or treatment of SLC6A1-related disorders, such as epilepsy, seizures, autism spectrum disorder, and related disorders.
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in basal ganglia neurons including striatal neurons, globus pallidus neurons, and substantia nigra neurons.
Provided herein are methods, devices, and systems comprising identification of a panel of gene markers useful for immune cell typing and identifying, detecting, and/or monitoring disease conditions in a subject in need thereof, including infections, cancer and autoimmune conditions. The methods also provide for assays for determining the levels of said markers prior to and after treatment of a condition in the subject, wherein the condition is impacted by one or more factors including age, occupation or environment of the subject.
C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
C12Q 1/6881 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
G16B 20/20 - Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
11.
ARTIFICIAL EXPRESSION CONSTRUCTS FOR MODULATING GENE EXPRESSION IN NEOCORTICAL LAYER 4 OR LAYER 5 INTRATELENCEPHALIC NEURONS
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in neocortical layer 4 (L4) intratelencephalic (IT) neurons and/or neocortical L5 IT neurons.
Seattle Children's Hospital d/b/a Seattle Children's Research Institute (USA)
Inventor
Mich, John K.
Lein, Edward Sebastian
Ting, Jonathan
Levi, Boaz P.
Hess, Erik
Kalume, Franck
Abstract
Selectively providing voltage-gated sodium channel function sufficient to rescue impaired Nav1.1 function to inhibitory neurons is described. Provided voltage-gated sodium channel function sufficient to rescue impaired Nav1.1 function in inhibitory neurons can be used to treat disorders such as epilepsy, and more particularly, Dravet Syndrome.
A61K 38/17 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans
A61K 9/00 - Medicinal preparations characterised by special physical form
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseasesGene therapy
Antisense oligonucleotides and artificial expression constructs for modulating RNA expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express RNA in GABAergic neurons. Antisense oligonucleotides and artificial expression constructs can be used to rescue sodium voltage-gated channel function to treat disorders such as epilepsy, and more particularly, Dravet Syndrome, among many other uses.
Artificial expression constructs for modulating gene expression in cardiomyocytes within targeted anatomical regions are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in cardiomyocytes within targeted anatomical regions including the heart atria and/or heart ventricles.
Provided herein are methods of preparing a biological sample for analysis. In some embodiments, the methods comprise cryopreserving the cells and assessing biological features. Also provided are methods of analyzing biological samples which have been prepared according to the steps of the present technology. In some embodiments, the methods of the present technology preserve native cell structures of biological samples relative to samples prepared by other methods.
SEATTLE CHILDREN'S HOSPITAL D/B/A SEATTLE CHILDREN'S RESEARCH INSTITUTE (USA)
Inventor
Gore, Bryan
Lein, Edward Sebastian
Levi, Boaz P.
Martinez, Refugio
Mich, John K.
Kalume, Franck
Abstract
Artificial expression constructs for the rescue of voltage-gated sodium channel function using intein-mediated reconstitution of the SCN1A encoded protein, voltage-gated sodium channel alpha subunit 1 (Nav1.1) are described. Rescued voltage-gated sodium channel function can be used to treat disorders such as epilepsy, and more particularly, Dravet Syndrome.
A61K 9/00 - Medicinal preparations characterised by special physical form
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseasesGene therapy
C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in the spinal cord spinal motor neurons including Spp1 spinal motor neurons, Parg spinal motor neurons, Ogdh1 spinal motor neurons, or ChAT spinal motor neurons; alpha motor neurons including Chodl spinal motor neurons; gamma motor neurons; spinal excitatory motor neurons including Mafa excitatory neurons, Esrrg Trhr excitatory neurons, Slc17a6 spinal cord excitatory neurons; spinal inhibitory neurons including Slc6a5 spinal cord inhibitory neurons; pan spinal neurons including Esrrg spinal motor neurons and pan spinal cord types; cerebrospinal fluid-contacting neurons including Poln spinal motor neurons; and spinal non-neuronal cells including astrocytes and oligodendrocytes.
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in GABAergic or glutamatergic neurons within the thalamus. In some cases, the artificial constructs can also be used to express synthetic genes or modify gene expression in neurons within the thalamus as well as a secondary cell type.
In various embodiments, provided are immune response signatures that can be used for the diagnosis, monitoring, and treatment of long-term diseases and inflammatory disorders caused by viral infections. In some embodiments, the viral infection is SARS-CoV-2 infection. In some embodiments, the long-term disease is post-acute sequelae of SARS-CoV-2 infection (PASC).
An expansion-assisted selective plane illumination microscope (ExA-SPIM) has diffraction- limited and aberration-free performance over a large field of view (e.g., 85 mm2) and working distance (e.g., 35 mm). ExA-SPIM enables molecular imaging across three- dimensional samples with high imaging throughput for applications in biology and neuroscience. Combined with new tissue clearing and expansion methods, ExA-SPIM allows nanoscale imaging of centimeter-scale samples, including entire mouse brains, with diffraction-limited resolutions and high contrast without sectioning. ExA-SPIM can be used to reconstruct individual neurons across the mouse brain, image cortico-spinal neurons in the macaque motor cortex, and trace axons in human white matter.
G01N 21/63 - Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in claustrum neurons including L6 IT Car3 neurons.
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseasesGene therapy
C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
22.
Removing independent noise using DeepInterpolation
A facility for transforming a subject data item sequence is described. The facility accesses a trained relationship model. For each of a plurality of subject items of the subject data item sequence, the facility: selects a first contiguous series of items of the subject data item sequence immediately before the subject data item; selects a second contiguous series of items of the subject data item sequence immediately after the subject data item; and applies the trained relationship model to the selected first and second contiguous series of data items to obtain a denoised version of the subject data item. The facility then assembles the obtained denoised subject data items into a denoised data item sequence.
Artificial expression constructs for modulating gene expression in GABAergic neurons and astrocytes are described. The artificial expression constructs can be used to express SLC6A1 for the treatment of SLC6A1-associated disorders, among other uses.
Electrostimulating waveforms offering simultaneous and controllable cell-type-specific entrainment are described. The waveforms selectively entrain excitatory versus inhibitory cortical and hippocampal neurons including pyramidal neurons, parvalbumin neurons, and somatostatin neurons. The embodiment provides targeted electrical stimulation (ES) entrainment of excitatory versus inhibitory neurons. For example, the current disclosure provides methods of selectively entraining excitatory neurons with ES frequencies below 30 Hertz (Hz) and in particular embodiments to frequencies below 15 Hz, such as 8 Hz and 4 Hz. The current disclosure also provides methods of selectively entraining inhibitory neurons to ES frequencies of at least 30 Hz and depending on the type of inhibitory neuron, utilizing a frequency that is 30-60 Hz or a frequency that is greater than 100 Hz.
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in chandelier cells. Chandelier cells are a subtype of GABAergic interneurons that that have been implicated in disorders such as epilepsy and schizophrenia.
A01K 67/027 - New or modified breeds of vertebrates
A61P 25/28 - Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
To fully understand the biology of the brain, different cell types need to be distinguished and defined and, to further study them, artificial expression constructs that can label and perturb them need to be identified, in mouse, recombinase driver lines have been used to great effect to label cell populations that share marker gene expression. However, the creation, maintenance, and use of such lines that label cell types with high specificity can be costly, frequently requiring triple transgenic crosses, which yield a low frequency of experimental animals. Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in non-neuronal cells including oligodendrocytes, microglia, astrocytes, or endothelial cells.
A61P 25/28 - Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
The present invention provides various methods for screening one or more compounds, suitably using non-invasive visual methods and neural networks for generating predicted fluorescence images of cells, to assess an effect of the compound on the cell, as well as to classify a compound or to determine an activity of a compound. Also provided are systems and methods for carrying out such assessments.
Artificial expression constructs for modulating gene expression in striatal neurons are described. The artificial expression constructs can be used to express heterologous genes in striatal neurons including in striatal medium spiny neuron-pan, striatal medium spiny neuron-indirect pathway, striatal medium spiny neuron-direct pathway, striatal interneuron-cholinergic, and Drd3+ medium spiny neurons in olfactory tubercle. The artificial expression constructs can be used for many purposes, including to research and treat movement disorders such as Parkinson's disease and Huntington's disease.
A computing device, method, system, and instructions in a non-transitory computer-readable medium for performing image analysis on 3D microscopy images to predict localization and/or labeling of various structures or objects of interest, by predicting the location in such images at which a dye or other marker associated with such structures would appear. The computing device, method, and system receives sets of 3D images that include unlabeled images, such as transmitted light images or electron microscope images, and labeled images, such as images captured with fluorescence tagging. The computing device trains a statistical model to associate structures in the labeled images with the same structures in the unlabeled light images. The processor further applies the statistical model to a new unlabeled image to generate a predictive labeled image that predicts the location of a structure of interest in the new image.
G06T 7/174 - SegmentationEdge detection involving the use of two or more images
G06T 7/187 - SegmentationEdge detection involving region growingSegmentationEdge detection involving region mergingSegmentationEdge detection involving connected component labelling
G06V 10/25 - Determination of region of interest [ROI] or a volume of interest [VOI]
G06V 10/50 - Extraction of image or video features by performing operations within image blocksExtraction of image or video features by using histograms, e.g. histogram of oriented gradients [HoG]Extraction of image or video features by summing image-intensity valuesProjection analysis
G06V 10/764 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using classification, e.g. of video objects
G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts
31.
ARTIFICIAL EXPRESSION CONSTRUCTS FOR MODULATING GENE EXPRESSION IN DOPAMINERGIC NEURONS
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in dopaminergic neurons of the central nervous system. Wherein, an artificial expression construct comprising (i) an enhancer is eHGT 888m.
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in dopaminergic neurons of the central nervous system. Wherein, an artificial expression construct comprising (i) an enhancer is eHGT 888m.
The present invention provides various methods for easily assessing cell quality of a cell production process, suitably using non-invasive visual methods and neural networks for generating predictive fluorescence images of cells to assess quality attributes. Also provided are systems and methods for carrying out such processes.
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in inhibitory neocortical GABAergic neurons including somatostatin GABAergic neurons, parvalbumin GABAergic neurons, vasointestinal peptide GABAergic neurons, Lamp5 GABAergic neurons, and in some instances astrocytes.
Provided herein are methods, devices, and systems comprising identification of a panel of gene markers useful for immune cell typing and identifying, detecting, and/or monitoring disease conditions in a subject in need thereof, including infections, cancer and autoimmune conditions. The methods also provide for assays for determining the levels of said markers prior to and after treatment of a condition in the subject, wherein the condition is impacted by one or more factors including age, occupation or environment of the subject.
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
G16B 25/10 - Gene or protein expression profilingExpression-ratio estimation or normalisation
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
C12Q 1/37 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase involving peptidase or proteinase
36.
ARTIFICIAL EXPRESSION CONSTRUCTS FOR MODULATING GENE EXPRESSION IN NEOCORTICAL LAYER 4 OR LAYER 5 INTRATELENCEPHALIC NEURONS
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in neocortical layer 4 (L4) intratelencephalic (IT) neurons and/or neocortical L5 IT neurons.
C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseasesGene therapy
A61P 25/28 - Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
37.
ARTIFICIAL EXPRESSION CONSTRUCTS FOR SELECTIVELY MODULATING GENE EXPRESSION IN NEOCORTICAL LAYER 5 GLUTAMATERGIC NEURONS
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. Particularly, the artificial expression constructs can be used to selectively express synthetic genes and/or modify gene expression in neocortical glutamatergic layer 5 neurons, such as glutamatergic layer 5 extratelencephalic-projecting (L5 ET) neurons.
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in astrocytes, oligodendrocytes, microglia, pericytes, SMC, or endothelial cells.
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in a cerebellar cell type and a secondary cell type.
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in a cerebellar cell type and a secondary cell type.
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in GABAergic or glutamatergic neurons within the thalamus. In some cases, the artificial constructs can also be used to express synthetic genes or modify gene expression in neurons within the thalamus as well as a secondary cell type.
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in GABAergic or glutamatergic neurons within the thalamus. In some cases, the artificial constructs can also be used to express synthetic genes or modify gene expression in neurons within the thalamus as well as a secondary cell type.
In various embodiments, provided are immune response signatures that can be used for the diagnosis, monitoring, and treatment of long-term diseases and inflammatory disorders caused by viral infections. In some embodiments, the viral infection is SARS-CoV-2 infection. In some embodiments, the long-term disease is post-acute sequelae of SARS-CoV-2 infection (PASC).
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
44.
ARTIFICIAL EXPRESSION CONSTRUCTS FOR MODULATING GENE EXPRESSION IN CLAUSTRUM NEURONS
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in claustrum neurons including L6 IT Car3 neurons.
Artificial expression constructs for modulating gene expression in GABAergic neurons and astrocytes are described. The artificial expression constructs can be used to express SLC6A1 for the treatment of SLC6A1-associated disorders, among other uses.
A61K 31/7088 - Compounds having three or more nucleosides or nucleotides
A61K 31/7105 - Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
A61K 31/7115 - Nucleic acids or oligonucleotides having modified bases, i.e. other than adenine, guanine, cytosine, uracil or thymine
A61K 31/712 - Nucleic acids or oligonucleotides having modified sugars, i.e. other than ribose or 2'-deoxyribose
A61K 31/7125 - Nucleic acids or oligonucleotides having modified internucleoside linkage, i.e. other than 3'-5' phosphodiesters
46.
ARTIFICIAL EXPRESSION CONSTRUCTS FOR SELECTIVELY MODULATING GENE EXPRESSION IN SELECTED NEURONAL CELL POPULATIONS
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in GABAergic neurons generally; and/or GABAergic neuron cell types such as lysosomal associated membrane protein 5 (Lamp5) neurons; vasoactive intestinal polypeptide-expressing (Vip) neurons; somatostatin (Sst) neurons; and/or parvalbumin (Pvalb) neuron cell types. Certain artificial expression constructs additionally drive selective gene expression in Layer 4 and/or layer 5 intratelencephalic (IT) neurons, deep cerebellar nuclear neurons or cerebellar Purkinje cells.
A system, apparatus and methods for generating database entries and tools for accessing and searching a database from an Ontology. Starting with an Ontology used to represent data and relationships between data, the system and methods described enable that data to be stored in a desired type of database and accessed using an API and search query generated from the Ontology. Embodiments provide a structure and process to implement a data access system or framework that can be used to unify and better understand information across an organization's entire set of data. Such a framework can help enable and improve the organization and discovery of knowledge, increase the value of existing data, and reduce complexity when developing next-generation applications.
Artificial expression constructs for modulating gene expression in GABAergic neurons and astrocytes are described. The artificial expression constructs can be used to express SLC6A1 for the treatment of SLC6A1-associated disorders, among other uses.
A61K 31/7088 - Compounds having three or more nucleosides or nucleotides
A61K 31/712 - Nucleic acids or oligonucleotides having modified sugars, i.e. other than ribose or 2'-deoxyribose
A61K 31/7105 - Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
A61K 31/7125 - Nucleic acids or oligonucleotides having modified internucleoside linkage, i.e. other than 3'-5' phosphodiesters
A61K 31/7115 - Nucleic acids or oligonucleotides having modified bases, i.e. other than adenine, guanine, cytosine, uracil or thymine
49.
ELECTRICAL STIMULATION ENTRAINMENT OF TARGETED NEURONAL CELL TYPES
Electrostimulating waveforms offering simultaneous and controllable cell-type-specific entrainment are described. The waveforms selectively entrain excitatory versus inhibitory cortical and hippocampal neurons including pyramidal neurons, parvalbumin neurons, and somatostatin neurons. The embodiment provides targeted electrical stimulation (ES) entrainment of excitatory versus inhibitory neurons. For example, the current disclosure provides methods of selectively entraining excitatory neurons with ES frequencies below 30 Hertz (Hz) and in particular embodiments to frequencies below 15 Hz, such as 8 Hz and 4 Hz. The current disclosure also provides methods of selectively entraining inhibitory neurons to ES frequencies of at least 30 Hz and depending on the type of inhibitory neuron, utilizing a frequency that is 30-60 Hz or a frequency that is greater than 100 Hz.
A61N 1/36 - Applying electric currents by contact electrodes alternating or intermittent currents for stimulation, e.g. heart pace-makers
A61M 21/00 - Other devices or methods to cause a change in the state of consciousnessDevices for producing or ending sleep by mechanical, optical, or acoustical means, e.g. for hypnosis
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in chandelier cells. Chandelier cells are a subtype of GABAergic interneurons that that have been implicated in disorders such as epilepsy and schizophrenia.
Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in chandelier cells. Chandelier cells are a subtype of GABAergic interneurons that that have been implicated in disorders such as epilepsy and schizophrenia.
Artificial expression constructs for modulating gene expression in striatal neurons are described. The artificial expression constructs can be used to express heterologous genes in striatal neurons including in striatal medium spiny neuron-pan, striatal medium spiny neuron-indirect pathway, striatal medium spiny neuron-direct pathway, striatal interneuron-cholinergic, and Drd3+ medium spiny neurons in olfactory tubercle. The artificial expression constructs can be used for many purposes, including to research and treat movement disorders such as Parkinson's disease and Huntington's disease.
The present invention provides various methods for easily assessing cell quality of a cell production process, suitably using non-invasive visual methods and neural networks for generating predictive fluorescence images of cells to assess quality attributes. Also provided are systems and methods for carrying out such processes.
Artificial expression constructs for modulating gene expression in striatal neurons are described. The artificial expression constructs can be used to express heterologous genes in striatal neurons including in striatal medium spiny neuron-pan, striatal medium spiny neuron-indirect pathway, striatal medium spiny neuron-direct pathway, striatal interneuron-cholinergic, and Drd3+ medium spiny neurons in olfactory tubercle. The artificial expression constructs can be used for many purposes, including to research and treat movement disorders such as Parkinson's disease and Huntington's disease.
The present invention provides various methods for screening one or more compounds, suitably using non-invasive visual methods and neural networks for generating predicted fluorescence images of cells, to assess an effect of the compound on the cell, as well as to classify a compound or to determine an activity of a compound. Also provided are systems and methods for carrying out such assessments.
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in excitatory cortical neurons, such as primarily within cortical layers 2/3, 4, 5, and 6 and including those with extratelencephalic (ET) projections, intratelencephalic (IT) projections, and pyramidal tract (PT) projections, among others.
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in inhibitory neocortical GABAergic neurons including somatostatin GABAergic neurons, parvalbumin GABAergic neurons, vasointestinal peptide GABAergic neurons, Lamp5 GABAergic neurons, and in some instances astrocytes.
Allen Institute, DBA ALLEN INSTITUTE FOR BRAIN SCIENCE; ALLEN INSTITUTE FOR CELL SCIENCE; ALLEN INSTITUTE FOR IMMUNOLOGY; ALLEN INSTITUTE FOR NEURAL DYNAMICS; PAUL G. ALLEN FRONTIERS GROUP ()
41 - Education, entertainment, sporting and cultural services
Goods & Services
Educational services, namely, providing classes, seminars, workshops and conferences in the fields of neuroscience and cell biology research, and distribution of informational materials therewith
60.
ARTIFICIAL EXPRESSION CONSTRUCTS FOR SELECTIVELY MODULATING GENE EXPRESSION IN INTERNEURONS
Seattle Children's Hospital d/b/a Seattle Children's Research Institute (USA)
Inventor
Ting, Jonathan
Levi, Boaz P.
Mich, John K.
Lein, Edward Sebastian
Kalume, Franck
Abstract
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in GABAergic interneurons.
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. Particularly, the artificial expression constructs can be used to selectively express synthetic genes and / or modify gene expression in neocortical glutamatergic layer 5 neurons, such as glutamatergic layer 5 extratelencephalic-projecting (L5 ET) neurons.
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. Particularly, the artificial expression constructs can be used to selectively express synthetic genes and / or modify gene expression in neocortical glutamatergic layer 5 neurons, such as glutamatergic layer 5 extratelencephalic-projecting (L5 ET) neurons.
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in astrocytes, oligodendrocytes, microglia, pericytes, SMC, or endothelial cells.
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in astrocytes, oligodendrocytes, microglia, pericytes, SMC, or endothelial cells.
A computing device, method, system, and instructions in a non-transitory computer-readable medium for performing image analysis on 3D microscopy images to predict localization and/or labeling of various structures or objects of interest, by predicting the location in such images at which a dye or other marker associated with such structures would appear. The computing device, method, and system receives sets of 3D images that include unlabeled images, such as transmitted light images or electron microscope images, and labeled images, such as images captured with fluorescence tagging. The computing device trains a statistical model to associate structures in the labeled images with the same structures in the unlabeled light images. The processor further applies the statistical model to a new unlabeled image to generate a predictive labeled image that predicts the location of a structure of interest in the new image.
A facility for transforming a subject data item sequence is described. The facility accesses a trained relationship model. For each of a plurality of subject items of the subject data item sequence, the facility: selects a first contiguous series of items of the subject data item sequence immediately before the subject data item; selects a second contiguous series of items of the subject data item sequence immediately after the subject data item; and applies the trained relationship model to the selected first and second contiguous series of data items to obtain a denoised version of the subject data item. The facility then assembles the obtained denoised subject data items into a denoised data item sequence.
An image transformation facility is described. The facility accesses a machine learning model trained to transform three-dimensional microscopy images in an imaging domain of a source image type into three-dimensional images in an imaging domain of a target image type. The facility also accesses a subject microscopy image in the imaging domain of the source image type. The facility applies the trained machine learning model to the subject microscopy image to obtain a transfer result image in the imaging domain of the target image type.
Selectively providing voltage-gated sodium channel function sufficient to rescue impaired Nav1.1 function to inhibitory neurons is described. Provided voltage-gated sodium channel function sufficient to rescue impaired Nav1.1 function in inhibitory neurons can be used to treat disorders such as epilepsy, and more particularly, Dravet Syndrome.
A61K 38/17 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans
A61K 9/00 - Medicinal preparations characterised by special physical form
A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseasesGene therapy
A system, apparatus and methods for generating database entries and tools for accessing and searching a database from an Ontology. Starting with an Ontology used to represent data and relationships between data, the system and methods described enable that data to be stored in a desired type of database and accessed using an API and search query generated from the Ontology. Embodiments provide a structure and process to implement a data access system or framework that can be used to unify and better understand information across an organization's entire set of data. Such a framework can help enable and improve the organization and discovery of knowledge, increase the value of existing data, and reduce complexity when developing next-generation applications.
A system includes a light source to generate an optical signal having a set of pulses at a first repetition rate. The system also includes a multiplexer circuit to generate a multiplexed optical signal from the optical signal n sets of pulses at a second repetition rate, where the n sets of pulses have different polarization states and are at the first repetition rate. The system also includes a focusing unit to split the multiplexed optical signal into n excitation signals to excite a sample. The system also includes an objective to receive the n excitation signals and to illuminate the sample. The objective and the focusing unit collectively focus each excitation signal of the n excitation signals on a different focal plane of the sample to generate a response signal. The system also includes a demultiplexer circuit to generate n emission signals based on the response signal.
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in GABAergic neurons generally; and/or GABAergic neuron cell types such as lysosomal associated membrane protein 5 (Lamp5) neurons; vasoactive intestinal polypeptide-expressing (Vip) neurons; somatostatin (Sst) neurons; and/or parvalbumin (Pvalb) neuron cell types. Certain artificial expression constructs additionally drive selective gene expression in Layer 4 and/or layer 5 intratelencephalic (IT) neurons, deep cerebellar nuclear neurons or cerebellar Purkinje cells.
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in GABAergic neurons generally; and/or GABAergic neuron cell types such as lysosomal associated membrane protein 5 (Lamp5) neurons; vasoactive intestinal polypeptide-expressing (Vip) neurons; somatostatin (Sst) neurons; and/or parvalbumin (Pvalb) neuron cell types. Certain artificial expression constructs additionally drive selective gene expression in Layer 4 and/or layer 5 intratelencephalic (IT) neurons, deep cerebellar nuclear neurons or cerebellar Purkinje cells.
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in excitatory cortical neurons, such as primarily within cortical layers 2/3, 4, 5, and 6 and including those with extratelencephalic (ET) projections, intratelencephalic (IT) projections, and pyramidal tract (PT) projections, among others.
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in excitatory cortical neurons, such as primarily within cortical layers 2/3, 4, 5, and 6 and including those with extratelencephalic (ET) projections, intratelencephalic (IT) projections, and pyramidal tract (PT) projections, among others.
A facility for identifying the boundaries of 3-dimensional structures in 3-dimensional images is described. For each of multiple 3-dimensional images, the facility receives results of a first attempt to identify boundaries of structures in the 3-dimensional image, and causes the results of the first attempt to be presented to a person. For each of a number of 3-dimensional images, the facility receives input generated by the person providing feedback on the results of the first attempt. The facility then uses the following to train a deep-learning network to identify boundaries of 3-dimensional structures in 3-dimensional images: at least a portion of the plurality of 3-dimensional images, at least a portion of the received results, and at least a portion of provided feedback.
A facility for identifying the boundaries of 3-dimensional structures in 3-dimensional images is described. For each of multiple 3-dimensional images, the facility receives results of a first attempt to identify boundaries of structures in the 3-dimensional image, and causes the results of the first attempt to be presented to a person. For each of a number of 3-dimensional images, the facility receives input generated by the person providing feedback on the results of the first attempt. The facility then uses the following to train a deep-learning network to identify boundaries of 3-dimensional structures in 3-dimensional images: at least a portion of the plurality of 3-dimensional images, at least a portion of the received results, and at least a portion of provided feedback.
A facility for identifying the boundaries of 3-dimensional structures in 3-dimensional images is described. For each of multiple 3-dimensional images, the facility receives results of a first attempt to identify boundaries of structures in the 3-dimensional image, and causes the results of the first attempt to be presented to a person. For each of a number of 3-dimensional images, the facility receives input generated by the person providing feedback on the results of the first attempt. The facility then uses the following to train a deep-learning network to identify boundaries of 3-dimensional structures in 3-dimensional images: at least a portion of the plurality of 3-dimensional images, at least a portion of the received results, and at least a portion of provided feedback.
SEATTLE CHILDREN'S HOSPITAL (D/B/A SEATTLE CHILDREN'S RESEARCH INSTITUTE) (USA)
Inventor
Ting, Jonathan
Levi, Boaz P.
Mich, John K.
Lein, Edward Sebastian
Kalume, Franck
Abstract
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in GABAergic interneurons.
SEATTLE CHILDREN'S HOSPITAL (D/B/A SEATTLE CHILDREN'S RESEARCH INSTITUTE) (USA)
Inventor
Ting, Jonathan
Levi, Boaz P.
Mich, John K.
Lein, Edward Sebastian
Kalume, Franck
Abstract
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in GABAergic interneurons.
A computing device, method, system, and instructions in a non-transitory computer-readable medium for performing image analysis on 3D microscopy images to predict localization and/or labeling of various structures or objects of interest, by predicting the location in such images at which a dye or other marker associated with such structures would appear. The computing device, method, and system receives sets of 3D images that include unlabeled images, such as transmitted light images or electron microscope images, and labeled images, such as images captured with fluorescence tagging. The computing device trains a statistical model to associate structures in the labeled images with the same structures in the unlabeled light images. The processor further applies the statistical model to a new unlabeled image to generate a predictive labeled image that predicts the location of a structure of interest in the new image.
The present invention provides stably tagged cells, including stem cells and methods for producing such cells comprising one or more tagged, differentially-expressed proteins using a gene editing system. The methods described herein enable the insertion of large fluorescent tags into a plurality of genomic loci to generate stem cells that are phenotypically and functional similar to the un-modified parent population. Stem cells produced by the methods described herein additionally retain the capacity to self-renew and differentiate into specialized cell types and can be used in assays and visualization of three-dimensional live cell imaging.
SEATTLE CHILDREN'S HOSPITAL D/B/A SEATTLE CHILDREN'S RESEARCH INSTITUTE (USA)
ALLEN INSTITUTE (USA)
Inventor
Mich, John K.
Lein, Edward Sebastian
Ting, Jonathan
Levi, Boaz P.
Hess, Erik
Kalume, Franck
Abstract
Selectively providing voltage-gated sodium channel function sufficient to rescue impaired Nav1.1 function to inhibitory neurons is described. Provided voltage-gated sodium channel function sufficient to rescue impaired Nav1.1 function in inhibitory neurons can be used to treat disorders such as epilepsy, and more particularly, Dravet Syndrome.
SEATTLE CHILDREN'S HOSPITAL D/B/A SEATTLE CHILDREN'S RESEARCH INSTITUTE (USA)
Inventor
Mich, John K.
Lein, Edward Sebastian
Ting, Jonathan
Levi, Boaz P.
Hess, Erik
Kalume, Franck
Abstract
Selectively providing voltage-gated sodium channel function sufficient to rescue impaired Nav1.1 function to inhibitory neurons is described. Provided voltage-gated sodium channel function sufficient to rescue impaired Nav1.1 function in inhibitory neurons can be used to treat disorders such as epilepsy, and more particularly, Dravet Syndrome.
A61K 31/7088 - Compounds having three or more nucleosides or nucleotides
A61K 31/7105 - Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
A61K 31/7115 - Nucleic acids or oligonucleotides having modified bases, i.e. other than adenine, guanine, cytosine, uracil or thymine
A61K 31/712 - Nucleic acids or oligonucleotides having modified sugars, i.e. other than ribose or 2'-deoxyribose
A61K 31/7125 - Nucleic acids or oligonucleotides having modified internucleoside linkage, i.e. other than 3'-5' phosphodiesters
Allen Institute, DBA Allen Institute for Brain Science; Allen Institute for Cell Science; Allen Institute for Immunology; Paul G. Allen Frontiers Group ()
09 - Scientific and electric apparatus and instruments
41 - Education, entertainment, sporting and cultural services
Goods & Services
Downloadable computer software for accessing medical and scientific research results Educational services, namely, providing classes, seminars, workshops and conferences in the fields of neuroscience and cell biology research, and distribution of informational materials therewith
Allen Institute, DBA ALLEN INSTITUTE FOR BRAIN SCIENCE; ALLEN INSTITUTE FOR CELL SCIENCE; ALLEN INSTITUTE FOR IMMUNOLOGY; PAUL G. ALLEN FRONTIERS GROUP ()
41 - Education, entertainment, sporting and cultural services
Goods & Services
Educational services, namely, providing classes, seminars, workshops and conferences in the fields of neuroscience and cell biology research, and distribution of informational materials therewith
Allen Institute, DBA Allen Institute for Brain Science; Allen Institute for Cell Science; Allen Institute for Immunology; Paul G. Allen Frontiers Group ()
41 - Education, entertainment, sporting and cultural services
42 - Scientific, technological and industrial services, research and design
Goods & Services
Educational services, namely, providing training, classes, workshops, seminars, symposia and conferences in the fields of human and animal biology research, and distributing informational materials in connection therewith Scientific and technological services, namely, science research information in the fields of science and medicine
87.
SYSTEMS, APPARATUSES AND METHODS FOR SIMULTANEOUS MULTI-PLANE IMAGING
A system includes a light source to generate an optical signal having a set of pulses at a first repetition rate. The system also includes a multiplexer circuit to generate a multiplexed optical signal from the optical signal n sets of pulses at a second repetition rate, where the n sets of pulses have different polarization states and are at the first repetition rate. The system also includes a focusing unit to split the multiplexed optical signal into n excitation signals to excite a sample. The system also includes an objective to receive the n excitation signals and to illuminate the sample. The objective and the focusing unit collectively focus each excitation signal of the n excitation signals on a different focal plane of the sample to generate a response signal. The system also includes a demultiplexer circuit to generate n emission signals based on the response signal.
A system includes a light source to generate an optical signal having a set of pulses at a first repetition rate. The system also includes a multiplexer circuit to generate a multiplexed optical signal from the optical signal n sets of pulses at a second repetition rate, where the n sets of pulses have different polarization states and are at the first repetition rate. The system also includes a focusing unit to split the multiplexed optical signal into n excitation signals to excite a sample. The system also includes an objective to receive the n excitation signals and to illuminate the sample. The objective and the focusing unit collectively focus each excitation signal of the n excitation signals on a different focal plane of the sample to generate a response signal. The system also includes a demultiplexer circuit to generate n emission signals based on the response signal.
A computing device, method, system, and instructions in a non-transitory computerreadable medium for performing image analysis on 3D microscopy images to predict localization and/or labeling of various structures or objects of interest, by predicting the location in such images at which a dye or other marker associated with such structures would appear. The computing device, method, and system receives sets of 3D images that include unlabeled images, such as transmitted light images or electron microscope images, and labeled images, such as images captured with fluorescence tagging. The computing device trains a statistical model to associate structures in the labeled images with the same structures in the unlabeled light images. The processor further applies the statistical model to a new unlabeled image to generate a predictive labeled image that predicts the location of a structure of interest in the new image.
A computing device, method, system, and instructions in a non-transitory computerreadable medium for performing image analysis on 3D microscopy images to predict localization and/or labeling of various structures or objects of interest, by predicting the location in such images at which a dye or other marker associated with such structures would appear. The computing device, method, and system receives sets of 3D images that include unlabeled images, such as transmitted light images or electron microscope images, and labeled images, such as images captured with fluorescence tagging. The computing device trains a statistical model to associate structures in the labeled images with the same structures in the unlabeled light images. The processor further applies the statistical model to a new unlabeled image to generate a predictive labeled image that predicts the location of a structure of interest in the new image.
A61B 5/0275 - Measuring blood flow using tracers, e.g. dye dilution
G06K 9/66 - Methods or arrangements for recognition using electronic means using simultaneous comparisons or correlations of the image signals with a plurality of references, e.g. resistor matrix references adjustable by an adaptive method, e.g. learning
91.
SYSTEMS, DEVICES, AND METHODS FOR IMAGE PROCESSING TO GENERATE AN IMAGE HAVING PREDICTIVE TAGGING
A computing device, method, system, and instructions in a non-transitory computer-readable medium for performing image analysis on 3D microscopy images to predict localization and/or labeling of various structures or objects of interest, by predicting the location in such images at which a dye or other marker associated with such structures would appear. The computing device, method, and system receives sets of 3D images that include unlabeled images, such as transmitted light images or electron microscope images, and labeled images, such as images captured with fluorescence tagging. The computing device trains a statistical model to associate structures in the labeled images with the same structures in the unlabeled light images. The processor further applies the statistical model to a new unlabeled image to generate a predictive labeled image that predicts the location of a structure of interest in the new image.
The present invention provides stably tagged stem cells and methods for producing stem cells comprising one or more tagged proteins using a gene editing system. The methods described herein enable the insertion of large fluorescent tags into a plurality of genomic loci to generate stem cells that are phenotypically and functional similar to the un-modified parent population. Stem cells produced by the methods described herein additionally retain the capacity to self-renew and differentiate into specialized cell types and can be used in assays and visualization of three- dimensional live cell imaging.
09 - Scientific and electric apparatus and instruments
41 - Education, entertainment, sporting and cultural services
42 - Scientific, technological and industrial services, research and design
Goods & Services
Computer software for accessing medical and scientific research results downloadable via the internet Educational services, namely, providing training, classes, workshops, seminars and conferences in the field of cell biology research, and distributing informational materials in connection therewith Medical and scientific research, namely, providing temporary use of non-downloadable computer software for accessing medical and scientific research results
09 - Scientific and electric apparatus and instruments
41 - Education, entertainment, sporting and cultural services
42 - Scientific, technological and industrial services, research and design
Goods & Services
Computer software for accessing medical and scientific research results downloadable via the internet Educational services, namely, providing training, classes, workshops, seminars and conferences in the field of cell biology research, and distributing informational materials in connection therewith Medical and scientific research, namely, providing temporary use of non-downloadable computer software for accessing medical and scientific research results
09 - Scientific and electric apparatus and instruments
41 - Education, entertainment, sporting and cultural services
42 - Scientific, technological and industrial services, research and design
Goods & Services
Computer software for accessing medical and scientific research results downloadable via the internet Educational services, namely, providing classes, seminars, workshops and conferences in the fields of neuroscience and cell biology research, and distribution of informational materials therewith Medical and scientific research, namely, providing temporary use of non-downloadable software for accessing medical and scientific results
96.
METHODS TO ISOLATE HIGH QUALITY RNA FROM FIXED SAMPLES
Some aspects of this disclosure provide compositions, methods, and kits for isolating RNA from fixed biological samples. Such compositions, methods, and kits are useful for isolating intact (e.g., not degraded or fragmented) RNA, for example, RNA having an RNA integrity number (RIN) ≥ 8.0.
ALLEN INSTITUTE, DBA ALLEN INSTITUTE FOR BRAIN SCIENCE; ALLEN INSTITUTE FOR CELL SCIENCE; ALLEN INSTITUTE FOR IMMUNOLOGY; ALLEN INSTITUTE FOR NEURAL DYNAMICS; PAUL G. ALLEN FRONTIERS GROUP ()
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Computer software for accessing medical and scientific research results downloadable via the internet Medical and scientific research, namely, providing temporary use of non-downloadable software for accessing medical and scientific results
42 - Scientific, technological and industrial services, research and design
Goods & Services
A house mark for medical and scientific research, namely, providing temporary use of non-downloadable computer software for accessing medical and scientific research results
42 - Scientific, technological and industrial services, research and design
Goods & Services
A house mark for medical and scientific research, namely, providing temporary use of non-downloadable computer software for accessing medical and scientific research results
ALLEN INSTITUTE, DBA ALLEN INSTITUTE FOR BRAIN SCIENCE; ALLEN INSTITUTE FOR CELL SCIENCE; ALLEN INSTITUTE FOR IMMUNOLOGY; PAUL G. ALLEN FRONTIERS GROUP ()
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Computer software for accessing medical and scientific research results downloadable via the internet Medical and scientific research, namely, providing temporary use of non-downloadable software for accessing medical and scientific results
