The invention refers to a container (1) that can be used for storing, transporting and for inoculating silk-free pupae. The container (1) comprises a tray (2) having a substantially flat surface (5) and a plurality of wells (4) formed on the surface (5), wherein each well (4) configured for accommodating a pupa (8). The container (1) also comprises a lid (3) having a plurality of openings (6), wherein the tray (2) and the lid (3) are configured to be coupled to each other, such as the lid (3) is placed on the flat surface (5), at least partially, closing the wells (4). The wells (4) and the openings (6) are arranged, such as when the tray (2) and the lid (3) are 10 coupled together, each well (4) is accessible through an opening (6). The container is stackable for an optimum and cost-efficient secure transportation.
The invention refers to a rearing boxes for growing insects from egg to the pupa stage. The insect rearing box (1) comprises: a lateral wall (2a,2b,3a,3b) and top and bottom covers (4a,4b) detachably coupled with the lateral wall to configure a insect rearing chamber (6). The box (1) further comprises a plurality of bars (5) placed inside the rearing chamber (6), and transversally arranged with respect to the top and bottom covers (4a,4b), wherein the bars (5) have top and bottom ends (5′,5″) adjacent respectively to the top and bottom covers (4a,4b), and wherein the bottom ends (5′) of the bars (5) are free ends. The rearing box (1) is suitable for the industrial production of insect pupae, preferably in an automatized production process, increasing the production rate as well as the quality and uniformity of the pupae.
The invention refers to a rearing boxes for growing insects from egg to the pupa stage. The insect rearing box (1) comprises: a lateral wall (2a,2b,3a,3b) and top and bottom covers (4a,4b) detachably coupled with the lateral wall to configure a insect rearing chamber (6). The box (1) further comprises a plurality of bars (5) placed inside the rearing chamber (6), and transversally arranged with respect to the top and bottom covers (4a,4b), wherein the bars (5) have top and bottom ends (5´,5´´) adjacent respectively to the top and bottom covers (4a,4b), and wherein the bottom ends (5´) of the bars (5) are free ends. The rearing box (1) is suitable for the industrial production of insect pupae, preferably in an automatized production process, increasing the production rate as well as the quality and uniformity of the pupae.
The invention refers to a container (1) that can be used for storing, transporting and for inoculating silk-free pupae. The container (1) comprises a tray (2) having a substantially flat surface (5) and a plurality of wells (4) formed on the surface (5), wherein each well (4) configured for accommodating a pupa (8). The container (1) also comprises a lid (3) having a plurality of openings (6), wherein the tray (2) and the lid (3) are configured to be coupled to each other, such as the lid (3) is placed on the flat surface (5), at least partially, closing the wells (4). The wells (4) and the openings (6) are arranged, such as when the tray (2) and the lid (3) are 10 coupled together, each well (4) is accessible through an opening (6). The container is stackable for an optimum and cost-efficient secure transportation.
The invention refers to a rearing boxes for growing insects from egg to the pupa stage. The insect rearing box (1) comprises: a lateral wall (2a,2b,3a,3b) and top and bottom covers (4a,4b) detachably coupled with the lateral wall to configure a insect rearing chamber (6). The box (1) further comprises a plurality of bars (5) placed inside the rearing chamber (6), and transversally arranged with respect to the top and bottom covers (4a,4b), wherein the bars (5) have top and bottom ends (5´,5´´) adjacent respectively to the top and bottom covers (4a,4b), and wherein the bottom ends (5´) of the bars (5) are free ends. The rearing box (1) is suitable for the industrial production of insect pupae, preferably in an automatized production process, increasing the production rate as well as the quality and uniformity of the pupae.
The invention refers to a container (1) that can be used for storing, transporting and for inoculating silk-free pupae. The container (1) comprises a tray (2) having a substantially flat surface (5) and a plurality of wells (4) formed on the surface (5), wherein each well (4) configured for accommodating a pupa (8). The container (1) also comprises a lid (3) having a plurality of openings (6), wherein the tray (2) and the lid (3) are configured to be coupled to each other, such as the lid (3) is placed on the flat surface (5), at least partially, closing the wells (4). The wells (4) and the openings (6) are arranged, such as when the tray (2) and the lid (3) are 10 coupled together, each well (4) is accessible through an opening (6). The container is stackable for an optimum and cost-efficient secure transportation.
T. ni) pupae. Moreover, the present invention is also directed to the pupae itself comprising baculovirus, pupae infected, transformed, transduced or transfected with baculoviruses or bacmids, as well as devices suitable for performing the methods of the present invention.
The present invention covers means and methods to increase the efficiency of recombinant protein expression, in particular to optimize the industrial production of recombinant proteins in insect pupae, particularly in Trichoplusia ni (T. ni) pupae. Moreover, the present invention is also directed to the pupae itself comprising baculovirus, pupae infected, transformed, transduced or transfected with baculovirases or bacmids, as well as devices suitable for performing the methods of the present invention.
The present invention covers means and methods to increase the efficiency of recombinant protein expression, in particular to optimize the industrial production of recombinant proteins in insect pupae, particularly in Trichoplusia ni (T. ni) pupae. Moreover, the present invention is also directed to the pupae itself comprising baculovirus, pupae infected, transformed, transduced or transfected with baculovirases or bacmids, as well as devices suitable for performing the methods of the present invention.
Reagents and methods are provided that allow for an improved expression of a recombinant protein. More specifically, the introduction of recombinant DNA elements into a host cell allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in cell viability and proliferation of the host cell, These recombinant DNA elements can be introduced into host cells, for example, via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and IE-1, transcriptional enhancer elements, such as the homologous region (hr) and promoters.
The present invention may be included in the field of biotechnology and it covers the improved production of recombinant proteins in insect cells or insect larvae as biofactories by a novel expression cassette. This expression cassette comprises nucleic acid sequences such as promoters, homologous regions (hr) as enhancers, and sequences encoding transcriptional regulators, for example, the baculovirus Ac-ie-01 cDNA, or any combination thereof, which are able to increase the quality and production efficiency of the recombinant proteins. Moreover, the present invention is also directed to the vectors themselves comprising the above mentioned nucleic acid sequences of the invention, cells or insects infected, transformed or transfected with those sequences or vectors, and methods for producing the recombinant proteins by using the aforesaid sequences, vectors, cells or insects.
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
C12N 7/00 - Viruses, e.g. bacteriophagesCompositions thereofPreparation or purification thereof
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
C12P 21/06 - Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
05 - Pharmaceutical, veterinary and sanitary products
07 - Machines and machine tools
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
Goods & Services
Pharmaceutical and veterinary preparations; sanitary preparations for medical purposes; dietetic food and substances adapted for medical or veterinary use, food for babies; dietary supplements for humans and animals; plasters, materials for dressings; material for stopping teeth, dental wax; disinfectants; preparations for destroying vermin; fungicides, herbicides; recombinant proteins; preparations comprising recombinant proteins; vaccines; diagnostic reagents. Machines, parts of machines and machine tools for the production of pharmaceutical and veterinary preparations, sanitary preparations for medical purposes, dietetic food and substances adapted for medical or veterinary use, recombinant insects, recombinant proteins, preparations comprising recombinant proteins, vaccines and diagnostic reagents; robots and parts of robots for use in the biotechnical industry. Production of recombinant proteins; production of preparations comprising recombinant proteins; production of vaccines; production of diagnostic reagents. Scientific and technological services and research and design relating thereto; industrial analysis and research services; design and development of computer hardware and software; development of recombinant proteins; development of preparations comprising recombinant proteins; development of vaccines; development of diagnostic reagents.
13.
Baculoviral DNA elements for the expression of recombinant proteins in a host insect
Reagents and methods are provided that allow for an improved expression of a recombinant protein in an insect, More specifically, the introduction of recombinant DNA elements into an insect larva allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in the survival rate after infection of the insect These recombinant DNA elements can be introduced, for example, into insect larvae via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and IE-1, transcriptional, enhancer elements, such as the homologous region (hr) and promoters.
A01N 63/00 - Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
The present invention may be included in the field of biotechnology and it covers the improved production of a subunit vaccine against porcine circovirus based on the capsid protein (Cap) in insect cells or insect larvae as biofactories by a novel expression cassette. This expression cassette comprises nucleic acid sequences such as promoters, homologous regions (hr) as enhancers, and sequences encoding transcriptional regulators, for example, the baculovirus Ac-ie-01 cDNA, or any combination thereof, which are able to increase the quality and production efficiency of a recombinant Cap protein from a porcine circovirus. Moreover, the present invention is also directed to the vectors themselves comprising the above mentioned nucleic acid sequences of the invention, cells or insects infected, transformed or transfected with those sequences or vectors, and methods for producing the Cap protein by using the aforesaid sequences, vectors, cells or insects.
The present invention may be included in the field of biotechnology and it covers the improved production of recombinant proteins in insect cells or insect larvae as biofactories by a novel expression cassette. This expression cassette comprises nucleic acid sequences such as promoters, homologous regions (hr) as enhancers, and sequences encoding transcriptional regulators, for example, the baculovirus Ac-ie-01 cDNA, or any combination thereof, which are able to increase the quality and production efficiency of the recombinant proteins. Moreover, the present invention is also directed to the vectors themselves comprising the above mentioned nucleic acid sequences of the invention, cells or insects infected, transformed or transfected with those sequences or vectors, and methods for producing the recombinant proteins by using the aforesaid sequences, vectors, cells or insects.
Reagents and methods are provided that allow for an improved expression of a recombinant protein in an insect. More specifically, the introduction of recombinant DNA elements into an insect larva allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in the survival rate after infection of the insect These recombinant DNA elements can be introduced, for example, into insect larvae via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and IE-1, transcriptional enhancer elements, such as the homologous region (hr) and promoters.
Reagents and methods are provided that allow for an improved expression of a recombinant protein in an insect, More specifically, the introduction of recombinant DNA elements into an insect larva allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in the survival rate after infection of the insect These recombinant DNA elements can be introduced, for example, into insect larvae via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and lE- 1, transcriptional, enhancer elements, such as the homologous region (hr) and promoters.
Reagents and methods are provided that allow for an improved expression of a recombinant protein. More specifically, the introduction of recombinant DNA elements into a host cell allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in cell viability and proliferation of the host cell, These recombinant DNA elements can be introduced into host cells, for example, via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and IE-1, transcriptional enhancer elements, such as the homologous region (hr) and promoters.
Reagents and methods are provided that allow for an improved expression of a recombinant protein. More specifically, the introduction of recombinant DNA elements into a host cell allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in cell viability and proliferation of the host cell, These recombinant DNA elements can be introduced into host cells, for example, via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and IE-1, transcriptional enhancer elements, such as the homologous region (hr) and promoters.
Reagents and methods are provided that allow for an improved expression of a recombinant protein. More specifically, the introduction of recombinant DNA elements into a host cell allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in cell viability and proliferation of the host cell. These recombinant DNA elements can be introduced into host cells, for example, via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and IE-1, transcriptional enhancer elements, such as the homologous region (hr) and promoters.
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Spain)
Alternative Gene Expression, S.L. (Spain)
Inventor
Martínez Escribano, José Angel
Wigdorovitz, Andrés
Gil Dones, Félix
Ostachuk, Agustin
Pèrez Filguera, Mariano
Dominguez, Javier
Núnez Serrano, Carmen
Dus Santos, Maria Josè
Alonso Marti, Covadonga
Abstract
Fusion protein with directioning of vaccinal antigens toward antigen-presenting cells and the applications thereof. This invention relates to a gene construct that comprises, operatively bound, at least one nucleotide sequence (A) that encodes a polypeptide with SEQ ID NO: 1, which has a region that recognizes the β chain of the class-II DR antigen present on the surface of antigen-presenting cells; and one nucleotide sequence (B) that encodes a vaccinal antigen of interest. Moreover, this invention relates to recombinant vectors useful for the expression of the gene construct of the invention, transgenic cells and plants transformed or transfected with said vectors, fusion proteins encoded by the gene construct of the invention and vaccines that comprise said fusion proteins.
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
C12N 5/02 - Propagation of single cells or cells in suspensionMaintenance thereofCulture media therefor
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
C07K 14/00 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof
22.
FUSION PROTEIN THAT DIRECTS VACCINE ANTIGENS TO ANTIGEN-PRESENTING CELLS, AND APPLICATIONS THEREOF
INSTITUTO NACIONAL DE INVESTIGACIÓN Y TECNOLOGÍA AGRARIA Y ALIMENTARIA (Spain)
ALTERNATIVE GENE EXPRESSION, S.L. (Spain)
Inventor
Martínez Escribano, José Ángel
Wigdorovitz, Andrés
Gil Dones, Félix
Ostachuk, Agustín
Pérez Filguera, Mariano
Domínguez, Javier
Núñez Serrano, Carmen
Dus Santos, María José
Alonso Martí, Covadonga
Abstract
Fusion protein that directs vaccine antigens to antigen-presenting cells, and applications thereof. The present invention relates to a gene construct that comprises, functionally linked, at least one nucleotide sequence (A) that encodes a polypeptide of SEQ ID No 1 that has a region that recognizes the β chain of the class II DR antigen present on the antigen-presenting cell surface; and a nucleotide sequence (B) that encodes a vaccine antigen of interest. Furthermore, the present invention relates to recombinant vectors that are useful in the expression of the gene construct of the invention, transgenic cells and plants transformed or transfected with said vectors, fusion proteins encoded by the gene construct of the invention and vaccines that comprise said fusion proteins.
C12N 15/62 - DNA sequences coding for fusion proteins
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
C07K 14/015 - Parvoviridae, e.g. feline panleukopenia virus, human parvovirus
C07K 14/18 - Togaviridae, e.g. flavivirus, pestivirus, yellow fever virus, hepatitis C virus, japanese encephalitis virus
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
INSTITUTO NACIONAL DE INVESTIGACIÓN Y TECNOLOGÍA AGRARIA Y ALIMENTARIA (Spain)
Inventor
Martínez Escribano, José Ángel
Gil Dones, Félix
Galindo, Inmaculada
Alonso Martí, Covadonga
Hernáez De La Plaza, Bruno
Gómez Casado, Eduardo
Abstract
The invention relates to a system based on a DNA construction comprising at least the following functionally bound sequences, namely: a nucleic acid sequence (A) containing the nucleotide sequence that codes for a product of interest, and a nucleic acid sequence (B) containing the nucleotide sequence that codes for a tetramerisation domain, such as the tetramerisation domain of protein p53, in which the 3' terminal of nucleic acid sequence A is bound to the 5' terminal of nucleic acid B. The system can be used to produce products of interest, for example recombinant multimeric peptides and proteins which can be used in vaccination, therapy, diagnosis, or industrial enzymes. Plants and insect larvae, transformed with the appropriate vectors such as to express the fused protein or peptide, are used as production systems. The expression of the 2L21 peptide from the canine parvovirus is illustrated in plants and the peptide binding to the dynein of protein p54 from African swine fever virus is illustrated in larvae, in both cases fused respectively to the p53 tetramerisation domain.
A61K 38/17 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans
G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
24.
DEVICE AND METHOD FOR THE MASS CULTURE OF INSECT LARVAE
The invention relates to a device and method for the mass culture of insect larvae. More specifically, the invention relates to a modular device intended for the culture of larvae, characterised in that it includes at least four independent removable modules. In addition, the invention relates to a larvae culture method, a virus production method and a recombinant protein production method in which said larvae are arranged in the appropriate growth state thereof inside the above-mentioned modular device.
INSTITUTO NACIONAL DE INVESTIGACIÓN Y TECNOLOGÍA AGRARIA Y ALIMENTARIA (Spain)
ALTERNATIVE GENE EXPRESSION, S.L. (Spain)
Inventor
Alonso Martí, Covadonga
Hernaez De La Plaza, Bruno
Martinez Escribano, José Angel
Abstract
New antiviral peptides interfering the binding of the virus to DLC8 A high number of pathogenic agents of viral origin use the dynein based intracellular transport machinery at some point of their infective cycie. The present invention consists of a new antiviral therapy consisting in the inhibition of viral infections produced by those virus that use the dynein system by mechanisms of interference mainly by preventing the interaction between the viral protein and the cellular DLC8 protein. The present invention discloses for the first time the blocking of the function of this interaction by peptides whose sequence comprises or consists of the totality or a partial sequence of the viral protein corresponding to the binding domain with DLC8.
05 - Pharmaceutical, veterinary and sanitary products
10 - Medical apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Pharmaceutical and veterinary preparations; sanitary preparations for medical purposes; dietetic substances adapted for medical use, food for babies; plasters, materials for dressings; material for stopping teeth, dental wax; disinfectants; preparations for destroying vermin; fungicides, herbicides. Surgical, medical, dental and veterinary apparatus and instruments, artificial limbs, eyes and teeth; orthopedic articles; suture materials. Scientific and technological services and research and design relating thereto; industrial analysis and research services; design and development of computer hardware and software.
