The invention refers to a container (1) that can be used for storing, transporting and for inoculating silk-free pupae. The container (1) comprises a tray (2) having a substantially flat surface (5) and a plurality of wells (4) formed on the surface (5), wherein each well (4) configured for accommodating a pupa (8). The container (1) also comprises a lid (3) having a plurality of openings (6), wherein the tray (2) and the lid (3) are configured to be coupled to each other, such as the lid (3) is placed on the flat surface (5), at least partially, closing the wells (4). The wells (4) and the openings (6) are arranged, such as when the tray (2) and the lid (3) are 10 coupled together, each well (4) is accessible through an opening (6). The container is stackable for an optimum and cost-efficient secure transportation.
The invention refers to a rearing boxes for growing insects from egg to the pupa stage. The insect rearing box (1) comprises: a lateral wall (2a,2b,3a,3b) and top and bottom covers (4a,4b) detachably coupled with the lateral wall to configure a insect rearing chamber (6). The box (1) further comprises a plurality of bars (5) placed inside the rearing chamber (6), and transversally arranged with respect to the top and bottom covers (4a,4b), wherein the bars (5) have top and bottom ends (5′,5″) adjacent respectively to the top and bottom covers (4a,4b), and wherein the bottom ends (5′) of the bars (5) are free ends. The rearing box (1) is suitable for the industrial production of insect pupae, preferably in an automatized production process, increasing the production rate as well as the quality and uniformity of the pupae.
The invention refers to a rearing boxes for growing insects from egg to the pupa stage. The insect rearing box (1) comprises: a lateral wall (2a,2b,3a,3b) and top and bottom covers (4a,4b) detachably coupled with the lateral wall to configure a insect rearing chamber (6). The box (1) further comprises a plurality of bars (5) placed inside the rearing chamber (6), and transversally arranged with respect to the top and bottom covers (4a,4b), wherein the bars (5) have top and bottom ends (5´,5´´) adjacent respectively to the top and bottom covers (4a,4b), and wherein the bottom ends (5´) of the bars (5) are free ends. The rearing box (1) is suitable for the industrial production of insect pupae, preferably in an automatized production process, increasing the production rate as well as the quality and uniformity of the pupae.
The invention refers to a container (1) that can be used for storing, transporting and for inoculating silk-free pupae. The container (1) comprises a tray (2) having a substantially flat surface (5) and a plurality of wells (4) formed on the surface (5), wherein each well (4) configured for accommodating a pupa (8). The container (1) also comprises a lid (3) having a plurality of openings (6), wherein the tray (2) and the lid (3) are configured to be coupled to each other, such as the lid (3) is placed on the flat surface (5), at least partially, closing the wells (4). The wells (4) and the openings (6) are arranged, such as when the tray (2) and the lid (3) are 10 coupled together, each well (4) is accessible through an opening (6). The container is stackable for an optimum and cost-efficient secure transportation.
The invention refers to a rearing boxes for growing insects from egg to the pupa stage. The insect rearing box (1) comprises: a lateral wall (2a,2b,3a,3b) and top and bottom covers (4a,4b) detachably coupled with the lateral wall to configure a insect rearing chamber (6). The box (1) further comprises a plurality of bars (5) placed inside the rearing chamber (6), and transversally arranged with respect to the top and bottom covers (4a,4b), wherein the bars (5) have top and bottom ends (5´,5´´) adjacent respectively to the top and bottom covers (4a,4b), and wherein the bottom ends (5´) of the bars (5) are free ends. The rearing box (1) is suitable for the industrial production of insect pupae, preferably in an automatized production process, increasing the production rate as well as the quality and uniformity of the pupae.
The invention refers to a container (1) that can be used for storing, transporting and for inoculating silk-free pupae. The container (1) comprises a tray (2) having a substantially flat surface (5) and a plurality of wells (4) formed on the surface (5), wherein each well (4) configured for accommodating a pupa (8). The container (1) also comprises a lid (3) having a plurality of openings (6), wherein the tray (2) and the lid (3) are configured to be coupled to each other, such as the lid (3) is placed on the flat surface (5), at least partially, closing the wells (4). The wells (4) and the openings (6) are arranged, such as when the tray (2) and the lid (3) are 10 coupled together, each well (4) is accessible through an opening (6). The container is stackable for an optimum and cost-efficient secure transportation.
T. ni) pupae. Moreover, the present invention is also directed to the pupae itself comprising baculovirus, pupae infected, transformed, transduced or transfected with baculoviruses or bacmids, as well as devices suitable for performing the methods of the present invention.
A01K 67/033 - NÉCESSITÉS COURANTES DE LA VIE ÉLEVAGE; CHASSE; PIÉGEAGE; PÊCHE ÉLEVAGE; AVICULTURE; APICULTURE; PISCICULTURE; PÊCHE; OBTENTION D'ANIMAUX, NON PRÉVUE AILLEURS; NOUVELLES RACES D'ANIMAUX Élevage ou obtention d'animaux, non prévus ailleurs; Nouvelles races d'animaux Élevage ou obtention d'invertébrés; Nouvelles races d'invertébrés
A61M 5/20 - Seringues automatiques, p. ex. avec tige de piston actionnée automatiquement, avec injection automatique de l'aiguille, à remplissage automatique
The present invention covers means and methods to increase the efficiency of recombinant protein expression, in particular to optimize the industrial production of recombinant proteins in insect pupae, particularly in Trichoplusia ni (T. ni) pupae. Moreover, the present invention is also directed to the pupae itself comprising baculovirus, pupae infected, transformed, transduced or transfected with baculovirases or bacmids, as well as devices suitable for performing the methods of the present invention.
A01K 67/033 - NÉCESSITÉS COURANTES DE LA VIE ÉLEVAGE; CHASSE; PIÉGEAGE; PÊCHE ÉLEVAGE; AVICULTURE; APICULTURE; PISCICULTURE; PÊCHE; OBTENTION D'ANIMAUX, NON PRÉVUE AILLEURS; NOUVELLES RACES D'ANIMAUX Élevage ou obtention d'animaux, non prévus ailleurs; Nouvelles races d'animaux Élevage ou obtention d'invertébrés; Nouvelles races d'invertébrés
A61M 5/20 - Seringues automatiques, p. ex. avec tige de piston actionnée automatiquement, avec injection automatique de l'aiguille, à remplissage automatique
B01L 99/00 - Matière non prévue dans les autres groupes de la présente sous-classe
C12N 15/85 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules animales
The present invention covers means and methods to increase the efficiency of recombinant protein expression, in particular to optimize the industrial production of recombinant proteins in insect pupae, particularly in Trichoplusia ni (T. ni) pupae. Moreover, the present invention is also directed to the pupae itself comprising baculovirus, pupae infected, transformed, transduced or transfected with baculovirases or bacmids, as well as devices suitable for performing the methods of the present invention.
A01K 67/033 - NÉCESSITÉS COURANTES DE LA VIE ÉLEVAGE; CHASSE; PIÉGEAGE; PÊCHE ÉLEVAGE; AVICULTURE; APICULTURE; PISCICULTURE; PÊCHE; OBTENTION D'ANIMAUX, NON PRÉVUE AILLEURS; NOUVELLES RACES D'ANIMAUX Élevage ou obtention d'animaux, non prévus ailleurs; Nouvelles races d'animaux Élevage ou obtention d'invertébrés; Nouvelles races d'invertébrés
C12N 15/85 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules animales
A61M 5/20 - Seringues automatiques, p. ex. avec tige de piston actionnée automatiquement, avec injection automatique de l'aiguille, à remplissage automatique
B01L 99/00 - Matière non prévue dans les autres groupes de la présente sous-classe
10.
Baculoviral DNA elements for the expression of recombinant proteins in a host cell
Reagents and methods are provided that allow for an improved expression of a recombinant protein. More specifically, the introduction of recombinant DNA elements into a host cell allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in cell viability and proliferation of the host cell, These recombinant DNA elements can be introduced into host cells, for example, via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and IE-1, transcriptional enhancer elements, such as the homologous region (hr) and promoters.
The present invention may be included in the field of biotechnology and it covers the improved production of recombinant proteins in insect cells or insect larvae as biofactories by a novel expression cassette. This expression cassette comprises nucleic acid sequences such as promoters, homologous regions (hr) as enhancers, and sequences encoding transcriptional regulators, for example, the baculovirus Ac-ie-01 cDNA, or any combination thereof, which are able to increase the quality and production efficiency of the recombinant proteins. Moreover, the present invention is also directed to the vectors themselves comprising the above mentioned nucleic acid sequences of the invention, cells or insects infected, transformed or transfected with those sequences or vectors, and methods for producing the recombinant proteins by using the aforesaid sequences, vectors, cells or insects.
C07H 21/04 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le désoxyribosyle comme radical saccharide
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
C12N 7/00 - Virus, p. ex. bactériophagesCompositions les contenantLeur préparation ou purification
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
C12P 21/06 - Préparation de peptides ou de protéines préparés par hydrolyse d'une liaison peptidique, p. ex. hydrolysats
05 - Produits pharmaceutiques, vétérinaires et hygièniques
07 - Machines et machines-outils
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Pharmaceutical and veterinary preparations; sanitary preparations for medical purposes; dietetic food and substances adapted for medical or veterinary use, food for babies; dietary supplements for humans and animals; plasters, materials for dressings; material for stopping teeth, dental wax; disinfectants; preparations for destroying vermin; fungicides, herbicides; recombinant proteins; preparations comprising recombinant proteins; vaccines; diagnostic reagents. Machines, parts of machines and machine tools for the production of pharmaceutical and veterinary preparations, sanitary preparations for medical purposes, dietetic food and substances adapted for medical or veterinary use, recombinant insects, recombinant proteins, preparations comprising recombinant proteins, vaccines and diagnostic reagents; robots and parts of robots for use in the biotechnical industry. Production of recombinant proteins; production of preparations comprising recombinant proteins; production of vaccines; production of diagnostic reagents. Scientific and technological services and research and design relating thereto; industrial analysis and research services; design and development of computer hardware and software; development of recombinant proteins; development of preparations comprising recombinant proteins; development of vaccines; development of diagnostic reagents.
13.
Baculoviral DNA elements for the expression of recombinant proteins in a host insect
Reagents and methods are provided that allow for an improved expression of a recombinant protein in an insect, More specifically, the introduction of recombinant DNA elements into an insect larva allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in the survival rate after infection of the insect These recombinant DNA elements can be introduced, for example, into insect larvae via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and IE-1, transcriptional, enhancer elements, such as the homologous region (hr) and promoters.
A01N 63/00 - Biocides, produits repoussant ou attirant les animaux nuisibles, ou régulateurs de croissance des végétaux, contenant des micro-organismes, des virus, des champignons microscopiques, des animaux ou des substances produites par, ou obtenues à partir de micro-organismes, de virus, de champignons microscopiques ou d'animaux, p. ex. enzymes ou produits de fermentation
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
The present invention may be included in the field of biotechnology and it covers the improved production of a subunit vaccine against porcine circovirus based on the capsid protein (Cap) in insect cells or insect larvae as biofactories by a novel expression cassette. This expression cassette comprises nucleic acid sequences such as promoters, homologous regions (hr) as enhancers, and sequences encoding transcriptional regulators, for example, the baculovirus Ac-ie-01 cDNA, or any combination thereof, which are able to increase the quality and production efficiency of a recombinant Cap protein from a porcine circovirus. Moreover, the present invention is also directed to the vectors themselves comprising the above mentioned nucleic acid sequences of the invention, cells or insects infected, transformed or transfected with those sequences or vectors, and methods for producing the Cap protein by using the aforesaid sequences, vectors, cells or insects.
The present invention may be included in the field of biotechnology and it covers the improved production of recombinant proteins in insect cells or insect larvae as biofactories by a novel expression cassette. This expression cassette comprises nucleic acid sequences such as promoters, homologous regions (hr) as enhancers, and sequences encoding transcriptional regulators, for example, the baculovirus Ac-ie-01 cDNA, or any combination thereof, which are able to increase the quality and production efficiency of the recombinant proteins. Moreover, the present invention is also directed to the vectors themselves comprising the above mentioned nucleic acid sequences of the invention, cells or insects infected, transformed or transfected with those sequences or vectors, and methods for producing the recombinant proteins by using the aforesaid sequences, vectors, cells or insects.
Reagents and methods are provided that allow for an improved expression of a recombinant protein in an insect. More specifically, the introduction of recombinant DNA elements into an insect larva allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in the survival rate after infection of the insect These recombinant DNA elements can be introduced, for example, into insect larvae via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and IE-1, transcriptional enhancer elements, such as the homologous region (hr) and promoters.
Reagents and methods are provided that allow for an improved expression of a recombinant protein in an insect, More specifically, the introduction of recombinant DNA elements into an insect larva allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in the survival rate after infection of the insect These recombinant DNA elements can be introduced, for example, into insect larvae via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and lE- 1, transcriptional, enhancer elements, such as the homologous region (hr) and promoters.
Reagents and methods are provided that allow for an improved expression of a recombinant protein. More specifically, the introduction of recombinant DNA elements into a host cell allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in cell viability and proliferation of the host cell, These recombinant DNA elements can be introduced into host cells, for example, via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and IE-1, transcriptional enhancer elements, such as the homologous region (hr) and promoters.
Reagents and methods are provided that allow for an improved expression of a recombinant protein. More specifically, the introduction of recombinant DNA elements into a host cell allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in cell viability and proliferation of the host cell, These recombinant DNA elements can be introduced into host cells, for example, via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and IE-1, transcriptional enhancer elements, such as the homologous region (hr) and promoters.
Reagents and methods are provided that allow for an improved expression of a recombinant protein. More specifically, the introduction of recombinant DNA elements into a host cell allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in cell viability and proliferation of the host cell. These recombinant DNA elements can be introduced into host cells, for example, via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and IE-1, transcriptional enhancer elements, such as the homologous region (hr) and promoters.
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Espagne)
Alternative Gene Expression, S.L. (Espagne)
Inventeur(s)
Martínez Escribano, José Angel
Wigdorovitz, Andrés
Gil Dones, Félix
Ostachuk, Agustin
Pèrez Filguera, Mariano
Dominguez, Javier
Núnez Serrano, Carmen
Dus Santos, Maria Josè
Alonso Marti, Covadonga
Abrégé
Fusion protein with directioning of vaccinal antigens toward antigen-presenting cells and the applications thereof. This invention relates to a gene construct that comprises, operatively bound, at least one nucleotide sequence (A) that encodes a polypeptide with SEQ ID NO: 1, which has a region that recognizes the β chain of the class-II DR antigen present on the surface of antigen-presenting cells; and one nucleotide sequence (B) that encodes a vaccinal antigen of interest. Moreover, this invention relates to recombinant vectors useful for the expression of the gene construct of the invention, transgenic cells and plants transformed or transfected with said vectors, fusion proteins encoded by the gene construct of the invention and vaccines that comprise said fusion proteins.
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
C12N 5/02 - Propagation de cellules individuelles ou de cellules en suspensionLeur conservationMilieux de culture à cet effet
C12N 15/00 - Techniques de mutation ou génie génétiqueADN ou ARN concernant le génie génétique, vecteurs, p. ex. plasmides, ou leur isolement, leur préparation ou leur purificationUtilisation d'hôtes pour ceux-ci
C07H 21/02 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques avec le ribosyle comme radical saccharide
C07K 14/00 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés
22.
FUSION PROTEIN THAT DIRECTS VACCINE ANTIGENS TO ANTIGEN-PRESENTING CELLS, AND APPLICATIONS THEREOF
INSTITUTO NACIONAL DE INVESTIGACIÓN Y TECNOLOGÍA AGRARIA Y ALIMENTARIA (Espagne)
ALTERNATIVE GENE EXPRESSION, S.L. (Espagne)
Inventeur(s)
Martínez Escribano, José Ángel
Wigdorovitz, Andrés
Gil Dones, Félix
Ostachuk, Agustín
Pérez Filguera, Mariano
Domínguez, Javier
Núñez Serrano, Carmen
Dus Santos, María José
Alonso Martí, Covadonga
Abrégé
Fusion protein that directs vaccine antigens to antigen-presenting cells, and applications thereof. The present invention relates to a gene construct that comprises, functionally linked, at least one nucleotide sequence (A) that encodes a polypeptide of SEQ ID No 1 that has a region that recognizes the β chain of the class II DR antigen present on the antigen-presenting cell surface; and a nucleotide sequence (B) that encodes a vaccine antigen of interest. Furthermore, the present invention relates to recombinant vectors that are useful in the expression of the gene construct of the invention, transgenic cells and plants transformed or transfected with said vectors, fusion proteins encoded by the gene construct of the invention and vaccines that comprise said fusion proteins.
C12N 15/62 - Séquences d'ADN codant pour des protéines de fusion
C12N 15/82 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules végétales
A01H 5/00 - Angiospermes, c.-à-d. plantes à fleurs, caractérisées par leurs parties végétalesAngiospermes caractérisées autrement que par leur taxonomie botanique
C07K 14/015 - Parvoviridae, p. ex. virus de l'aleucémie féline, parvovirus humain
C07K 14/18 - Togaviridae, p. ex. flavivirus, virus de la peste, virus de la fièvre jaune, virus de l'hépatite C, virus de l'encéphalite japonaise
C07K 16/28 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains contre des récepteurs, des antigènes de surface cellulaire ou des déterminants de surface cellulaire
INSTITUTO NACIONAL DE INVESTIGACIÓN Y TECNOLOGÍA AGRARIA Y ALIMENTARIA (Espagne)
Inventeur(s)
Martínez Escribano, José Ángel
Gil Dones, Félix
Galindo, Inmaculada
Alonso Martí, Covadonga
Hernáez De La Plaza, Bruno
Gómez Casado, Eduardo
Abrégé
The invention relates to a system based on a DNA construction comprising at least the following functionally bound sequences, namely: a nucleic acid sequence (A) containing the nucleotide sequence that codes for a product of interest, and a nucleic acid sequence (B) containing the nucleotide sequence that codes for a tetramerisation domain, such as the tetramerisation domain of protein p53, in which the 3' terminal of nucleic acid sequence A is bound to the 5' terminal of nucleic acid B. The system can be used to produce products of interest, for example recombinant multimeric peptides and proteins which can be used in vaccination, therapy, diagnosis, or industrial enzymes. Plants and insect larvae, transformed with the appropriate vectors such as to express the fused protein or peptide, are used as production systems. The expression of the 2L21 peptide from the canine parvovirus is illustrated in plants and the peptide binding to the dynein of protein p54 from African swine fever virus is illustrated in larvae, in both cases fused respectively to the p53 tetramerisation domain.
A61K 38/17 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'animauxPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'humains
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
24.
DEVICE AND METHOD FOR THE MASS CULTURE OF INSECT LARVAE
The invention relates to a device and method for the mass culture of insect larvae. More specifically, the invention relates to a modular device intended for the culture of larvae, characterised in that it includes at least four independent removable modules. In addition, the invention relates to a larvae culture method, a virus production method and a recombinant protein production method in which said larvae are arranged in the appropriate growth state thereof inside the above-mentioned modular device.
INSTITUTO NACIONAL DE INVESTIGACIÓN Y TECNOLOGÍA AGRARIA Y ALIMENTARIA (Espagne)
ALTERNATIVE GENE EXPRESSION, S.L. (Espagne)
Inventeur(s)
Alonso Martí, Covadonga
Hernaez De La Plaza, Bruno
Martinez Escribano, José Angel
Abrégé
New antiviral peptides interfering the binding of the virus to DLC8 A high number of pathogenic agents of viral origin use the dynein based intracellular transport machinery at some point of their infective cycie. The present invention consists of a new antiviral therapy consisting in the inhibition of viral infections produced by those virus that use the dynein system by mechanisms of interference mainly by preventing the interaction between the viral protein and the cellular DLC8 protein. The present invention discloses for the first time the blocking of the function of this interaction by peptides whose sequence comprises or consists of the totality or a partial sequence of the viral protein corresponding to the binding domain with DLC8.
05 - Produits pharmaceutiques, vétérinaires et hygièniques
10 - Appareils et instruments médicaux
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Pharmaceutical and veterinary preparations; sanitary preparations for medical purposes; dietetic substances adapted for medical use, food for babies; plasters, materials for dressings; material for stopping teeth, dental wax; disinfectants; preparations for destroying vermin; fungicides, herbicides. Surgical, medical, dental and veterinary apparatus and instruments, artificial limbs, eyes and teeth; orthopedic articles; suture materials. Scientific and technological services and research and design relating thereto; industrial analysis and research services; design and development of computer hardware and software.
