The disclosure provides methods and systems for sample screening which may involve screening samples in presence and/or absence of effectors (e.g., encoded effector/compound libraries) for their potential effects on the sample or a target, in some cases, a sample comprising a cell or constituent of a cell, a target (e.g., an enzyme) in a cell or a target in a cell-free sample (e.g., in a biochemical assay with a purified target extracted from a cell). The disclosure further provides methods and systems for computational screening and predictive analysis.
C40B 20/04 - Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
C40B 30/04 - Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
C40B 50/16 - Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creationParticular methods of cleavage from the solid support involving encoding steps
The disclosure provides methods and systems for sample screening which may involve screening samples in presence and/or absence of effectors (e.g., encoded effector/compound libraries) for their potential effects on the sample and/or a target, in some cases, a sample comprising a cell or constituent of a cell, an enzyme in a cell or a target in a cell-free sample. The methods and systems presented herein can comprise miniaturized systems for high-throughput sample screening with vast applications including but not limited to cell screening and cell perturbation analysis including encoded effector library screening.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
C40B 20/04 - Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
3.
METHODS AND SYSTEMS FOR ENCODED EFFECTOR SCREENING
The disclosure provides methods and systems for sample screening which may involve screening samples in presence and/or absence of effectors (e.g., encoded effector/compound libraries) for their potential effects on the sample or a target, in some cases, a sample comprising a cell or constituent of a cell, a target (e.g., an enzyme) in a cell or a target in a cell-free sample (e.g., in a biochemical assay with a purified target extracted from a cell). A variety of encoding modalities including nucleic acid barcodes and optical barcodes are disclosed. The methods and systems presented herein can comprise miniaturized systems for high-throughput sample screening with vast applications including but not limited to cell screening and cell perturbation analysis such as encoded effector library screening.
C40B 60/02 - Integrated apparatus specially adapted for creating libraries, screening libraries and for identifying library members
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C07K 17/08 - Peptides being immobilised on, or in, an organic carrier the carrier being a synthetic polymer
C08F 257/02 - Macromolecular compounds obtained by polymerising monomers on to polymers of aromatic monomers as defined in group on to polymers of styrene or alkyl-substituted styrenes
C08F 283/06 - Macromolecular compounds obtained by polymerising monomers on to polymers provided for in subclass on to polyethers, polyoxymethylenes or polyacetals
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C40B 20/04 - Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
C40B 30/04 - Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
Provided are methods and systems useful for screening large libraries of effector molecules. Such methods and systems are particularly useful in microfluidic systems and devices. The methods and systems provided herein utilize encoded effectors to screen large libraries of effectors.
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C40B 50/06 - Biochemical methods, e.g. using enzymes or whole viable microorganisms
C40B 50/16 - Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creationParticular methods of cleavage from the solid support involving encoding steps
Provided are methods and systems useful for screening large libraries of effector molecules. Such methods and systems are particularly useful in microfluidic systems and devices. The methods and systems provided herein utilize encoded effectors to screen large libraries of effectors.
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C40B 50/06 - Biochemical methods, e.g. using enzymes or whole viable microorganisms
C40B 50/16 - Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creationParticular methods of cleavage from the solid support involving encoding steps
Provided are methods and systems useful for screening large libraries of effector molecules. Such methods and systems are particularly useful in microfluidic systems and devices. The methods and systems provided herein utilize encoded effectors to screen large libraries of effectors.
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C40B 50/06 - Biochemical methods, e.g. using enzymes or whole viable microorganisms
C40B 50/16 - Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creationParticular methods of cleavage from the solid support involving encoding steps
Provided are methods and systems useful for screening large libraries of effector molecules. Such methods and systems are particularly useful in microfluidic systems and devices. The methods and systems provided herein utilize encoded effectors to screen large libraries of effectors.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C40B 50/06 - Biochemical methods, e.g. using enzymes or whole viable microorganisms
C40B 50/16 - Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creationParticular methods of cleavage from the solid support involving encoding steps
Provided are methods and systems useful for screening large libraries of effector molecules. Such methods and systems are particularly useful in microfluidic systems and devices. The methods and systems provided herein utilize encoded effectors to screen large libraries of effectors.
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C40B 50/06 - Biochemical methods, e.g. using enzymes or whole viable microorganisms
C40B 50/16 - Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creationParticular methods of cleavage from the solid support involving encoding steps
Provided are methods and systems useful for screening large libraries of effector molecules. Such methods and systems are particularly useful in microfluidic systems and devices. The methods and systems provided herein utilize encoded effectors to screen large libraries of effectors.
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C40B 50/06 - Biochemical methods, e.g. using enzymes or whole viable microorganisms
C40B 50/16 - Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creationParticular methods of cleavage from the solid support involving encoding steps
Provided are methods and systems useful for screening large libraries of effector molecules. Such methods and systems are particularly useful in microfluidic systems and devices. The methods and systems provided herein utilize encoded effectors to screen large libraries of effectors.
C40B 50/06 - Biochemical methods, e.g. using enzymes or whole viable microorganisms
C40B 50/16 - Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creationParticular methods of cleavage from the solid support involving encoding steps
C40B 20/04 - Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
C40B 30/08 - Methods of screening libraries by measuring catalytic activity
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Provided are methods and systems useful for screening large libraries of effector molecules. Such methods and systems are particularly useful in microfluidic systems and devices. The methods and systems provided herein utilize encoded effectors to screen large libraries of effectors.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C40B 50/06 - Biochemical methods, e.g. using enzymes or whole viable microorganisms
C40B 50/16 - Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creationParticular methods of cleavage from the solid support involving encoding steps
C40B 20/04 - Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
C40B 30/08 - Methods of screening libraries by measuring catalytic activity
Provided are methods and systems useful for screening large libraries of effector molecules. Such methods and systems are particularly useful in microfluidic systems and devices. The methods and systems provided herein utilize encoded effectors to screen large libraries of effectors.
A61K 47/64 - Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C40B 20/04 - Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
C40B 30/04 - Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
Provided are methods and systems useful for screening large libraries of effector molecules. Such methods and systems are particularly useful in microfluidic systems and devices. The methods and systems provided herein utilize encoded effectors to screen large libraries of effectors.
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C40B 20/04 - Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
C40B 30/04 - Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
C40B 30/06 - Methods of screening libraries by measuring effects on living organisms, tissues or cells
C40B 50/10 - Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creationParticular methods of cleavage from the liquid support involving encoding steps
C40B 70/00 - Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or barcodes
G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
Provided are methods and systems useful for screening large libraries of effector molecules. Such methods and systems are particularly useful in microfluidic systems and devices. The methods and systems provided herein utilize encoded effectors to screen large libraries of effectors.
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C40B 20/04 - Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
C40B 30/08 - Methods of screening libraries by measuring catalytic activity
C40B 50/06 - Biochemical methods, e.g. using enzymes or whole viable microorganisms
C40B 50/16 - Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creationParticular methods of cleavage from the solid support involving encoding steps
Provided are methods and systems useful for screening large libraries of effector molecules. Such methods and systems are particularly useful in microfluidic systems and devices. The methods and systems provided herein utilize encoded effectors to screen large libraries of effectors.
C40B 50/06 - Biochemical methods, e.g. using enzymes or whole viable microorganisms
C40B 50/16 - Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creationParticular methods of cleavage from the solid support involving encoding steps
C40B 20/04 - Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
C40B 30/08 - Methods of screening libraries by measuring catalytic activity
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Provided are methods and systems useful for screening large libraries of effector molecules. Such methods and systems are particularly useful in microfluidic systems and devices. The methods and systems provided herein utilize encoded effectors to screen large libraries of effectors.
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C40B 50/06 - Biochemical methods, e.g. using enzymes or whole viable microorganisms
C40B 50/16 - Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creationParticular methods of cleavage from the solid support involving encoding steps
C40B 20/04 - Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
C40B 30/08 - Methods of screening libraries by measuring catalytic activity
patents
