Abstract

One aspect described herein is a method of 3D printing a tissue model in a contained volume of photocrosslinkable bioink located on a print surface. The bioink may contain living cells. The method may comprise maintaining, with the print surface, a cell viability temperature of the contained volume; locating an objective lens vertically in a bottom working plane within the contained volume and successive working planes located above the bottom working plane by moving the lens vertically relative to the surface; and in the bottom working plane and each successive working plane, moving the voxel horizontally by directing the laser beam into the objective lens at different angles at different times; focusing, with the objective lens, at each different angle and time, the laser beam into a voxel; polymerizing, with the voxel, a portion of the contained volume at each horizontal location; and moving the contained volume horizontally.

IPC Classes  ?

• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• B29C 64/129 - Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified characterised by the energy source therefor, e.g. by global irradiation combined with a mask
• B33Y 10/00 - Processes of additive manufacturing
• B33Y 70/10 - Composites of different types of material, e.g. mixtures of ceramics and polymers or mixtures of metals and biomaterials
• B33Y 80/00 - Products made by additive manufacturing
• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
• C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
• C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
• C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions

Abstract

Aspects of pH responsive polymers are described. One exemplary aspect is a pH responsive GelMA polymer comprising a gelatin type A with selectively modified and unmodified functional groups. For example, this polymer may comprise: modified carboxyl groups, modified hydroxyl groups, unmodified carboxyl groups, unmodified hydroxyl groups, and unmodified amine groups; carboxyl and hydroxyl groups that have been methacrylated; or carboxyl, hydroxyl, and amine groups that were not methacrylated, wherein the modified and unmodified functional groups of Polymer A may be selected by controlling a pH level of its synthesizing reaction. Another exemplary aspect is a pH responsive GelMA polymer comprising a gelatin type B with selectively modified amine groups, modified hydroxyl groups, unmodified carboxyl groups, unmodified, hydroxyl groups, and unmodified amine groups. Related apparatus, compositions, methods, polymers, and systems are described.

IPC Classes  ?

• A61K 9/16 - AgglomeratesGranulatesMicrobeadlets
• A61K 33/242 - GoldCompounds thereof
• B01J 13/14 - Polymerisation, crosslinking
• C08H 1/06 - Macromolecular products derived from proteins derived from horn, hoofs, hair, skin, or leather
• C08L 89/06 - Products derived from waste materials, e.g. horn, hoof or hair derived from leather or skin

Abstract

Bioinks and methods of synthesizing, characterizing, and deploying bioinks are described. Compounds and methods of making the same are described. Method of synthesizing pH-responsive polymers are described. Different types of bioinks are described. For example, the described bioinks may comprise: one or more of the pH-responsive polymers; a photoinitiator; and a solvent. Methods preparing bioinks are described. Methods of determining characteristics of bioinks are described. Related apparatus, kits, methods, and systems also are described.

IPC Classes  ?

• C08H 1/00 - Macromolecular products derived from proteins
• B33Y 70/00 - Materials specially adapted for additive manufacturing
• C08B 37/08 - ChitinChondroitin sulfateHyaluronic acidDerivatives thereof
• C08J 3/075 - Macromolecular gels
• C08L 5/08 - ChitinChondroitin sulfateHyaluronic acidDerivatives thereof
• C08L 89/00 - Compositions of proteinsCompositions of derivatives thereof
• C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
• G01N 3/00 - Investigating strength properties of solid materials by application of mechanical stress
• G01N 3/42 - Investigating hardness or rebound hardness by performing impressions under a steady load by indentors, e.g. sphere, pyramid

Abstract

Aspects of pH responsive polymers are described. One exemplary aspect is a pH responsive GelMA polymer comprising a gelatin type A with selectively modified and unmodified functional groups. For example, this polymer may comprise: modified carboxyl groups, modified hydroxyl groups, unmodified carboxyl groups, unmodified hydroxyl groups, and unmodified amine groups; carboxyl and hydroxyl groups that have been methacrylated; or carboxyl, hydroxyl, and amine groups that were not methacrylated, wherein the modified and unmodified functional groups of Polymer A may be selected by controlling a pH level of its synthesizing reaction. Another exemplary aspect is a pH responsive GelMA polymer comprising a gelatin type B with selectively modified amine groups, modified hydroxyl groups, unmodified carboxyl groups, unmodified, hydroxyl groups, and unmodified amine groups. Related apparatus, compositions, methods, polymers, and systems are described.

IPC Classes  ?

• C08H 1/06 - Macromolecular products derived from proteins derived from horn, hoofs, hair, skin, or leather
• C08J 3/24 - Crosslinking, e.g. vulcanising, of macromolecules
• C08J 3/075 - Macromolecular gels
• C08L 89/06 - Products derived from waste materials, e.g. horn, hoof or hair derived from leather or skin

Goods & Services

Biologically compatible materials, namely, hydrogels, polymers, and related materials used for 3D bioprinting tissue models, vascular structures, scaffolds, polymeric networks, and/or biological tissue specimens capable of mimicking different types of tissues for scientific use. Pharmaceutical preparations and screening, namely, hydrogels, polymers, and related preparations and materials used for 3D bioprinting tissue models, vascular structures, scaffolds, polymeric networks, and/or biological tissue specimens capable of mimicking different types of tissues for medical use.

Goods & Services

(1) Biologically compatible materials, namely hydrogels, polymers, and related materials used for 3D tissue models, vascular structures, scaffolds, polymeric networks, and/or biological tissue specimens capable of mimicking different types of human tissues for medical use and/or scientific research; biological tissue cultures for medical purposes; tissue-regenerative pharmaceutical preparations; and biological products made from such material. (2) Pharmaceutical preparations and screening, namely hydrogels, polymers, and related preparations used for 3D bioprinting tissue models, vascular structures, scaffolds, polymeric networks, and/or biological tissue specimens capable of mimicking different types of human tissues for medical and scientific use; biological tissue cultures for medical purposes; tissue-regenerative pharmaceutical preparations; and pharmaceutical products made with such preparations. (1) Scientific research; biological research; scientific and technological consulting services and research and design relating thereto in the fields of cell biology and life science research; research, design and development of tools for tissue testing; research, design and development of instruments and devices for cell research; research, design and development for instruments and devices for 3D bioprinting; research, design and development of tools for 3D printing of biologic products, biological tissues and proteins.

Goods & Services

(1) Biologically compatible materials, namely, hydrogels, polymers, and related materials used for 3D bioprinting tissue models, vascular structures, scaffolds, polymeric networks, and/or biological tissue specimens capable of mimicking different types of tissues for scientific use. (2) Pharmaceutical preparations and screening, namely, hydrogels, polymers, and related preparations and materials used for 3D bioprinting tissue models, vascular structures, scaffolds, polymeric networks, and/or biological tissue specimens capable of mimicking different types of tissues for medical use.

Goods & Services

Biologically compatible materials, namely, hydrogels used to create polymeric networks for three-dimensional (3D) tissue models, vascular structures, scaffolds, and biological tissue specimens capable of mimicking different types of human tissues for medical use and scientific research; Biologically compatible materials, namely, polymers for use in three-dimensional (3D) tissue models, vascular structures, scaffolds, polymeric networks, and biological tissue specimens capable of mimicking different types of human tissues for medical use and scientific research; biological products for non-medical use, namely, reagents for scientific and medical research use Pharmaceutical preparations namely, a three-dimensional (3D) bioprinted drug delivery system comprised of hydrogel-based and polymer-based bioinks for the creation of biological tissues for medical purposes; biological tissue cultures for medical purposes; tissue-regenerative pharmaceutical preparations Scientific research; biological research; scientific and technological consulting services in the cell biology and life sciences industry, and research and design relating thereto; research, design and development of tools for tissue testing; research, design and development of instruments and devices for stem cell research; research, design and development of instruments and devices for three-dimensional (3D) bioprinting; research, design and development of tools for three-dimensional (3D)printing of biological products, biological tissues and proteins; Drug screening for medical purposes Drug screening for scientific research purposes

Goods & Services

Biologically compatible materials for three-dimensional (3D) bioprinted tissue models, namely, hydrogels and polymers used to create vascular structures, scaffolds, polymeric networks, and biological tissue specimens capable of mimicking different types of tissues for medical and scientific purposes Pharmaceutical preparations, namely, three-dimensional (3D) bioprinted drug delivery systems comprised of hydrogel-based medical dressings and polymer-based systems for the continuous release of a wide variety of therapeutic agents

Abstract

Aspects of pH responsive polymers are described. One exemplary aspect is a pH responsive GelMA polymer comprising a gelatin type A with selectively modified and unmodified functional groups. For example, this polymer may comprise: modified carboxyl groups, modified hydroxyl groups, unmodified carboxyl groups, unmodified hydroxyl groups, and unmodified amine groups; carboxyl and hydroxyl groups that have been methacrylated; or carboxyl, hydroxyl, and amine groups that were not methacrylated, wherein the modified and unmodified functional groups of Polymer A may be selected by controlling a pH level of its synthesizing reaction. Another exemplary aspect is a pH responsive GelMA polymer comprising a gelatin type B with selectively modified amine groups, modified hydroxyl groups, unmodified carboxyl groups, unmodified, hydroxyl groups, and unmodified amine groups. Related apparatus, compositions, methods, polymers, and systems are described.

IPC Classes  ?

• C08H 1/06 - Macromolecular products derived from proteins derived from horn, hoofs, hair, skin, or leather
• C08J 3/075 - Macromolecular gels
• C08L 89/06 - Products derived from waste materials, e.g. horn, hoof or hair derived from leather or skin