Abrégé

One aspect described herein is a method of 3D printing a tissue model in a contained volume of photocrosslinkable bioink located on a print surface. The bioink may contain living cells. The method may comprise maintaining, with the print surface, a cell viability temperature of the contained volume; locating an objective lens vertically in a bottom working plane within the contained volume and successive working planes located above the bottom working plane by moving the lens vertically relative to the surface; and in the bottom working plane and each successive working plane, moving the voxel horizontally by directing the laser beam into the objective lens at different angles at different times; focusing, with the objective lens, at each different angle and time, the laser beam into a voxel; polymerizing, with the voxel, a portion of the contained volume at each horizontal location; and moving the contained volume horizontally.

Classes IPC  ?

• C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
• B29C 64/129 - Procédés de fabrication additive n’utilisant que des matériaux liquides ou visqueux, p. ex. dépôt d’un cordon continu de matériau visqueux utilisant des couches de liquide à solidification sélective caractérisés par la source d'énergie à cet effet, p. ex. par irradiation globale combinée avec un masque
• B33Y 10/00 - Procédés de fabrication additive
• B33Y 70/10 - Composites de différents types de matériaux, p. ex. mélanges de céramiques et de polymères ou mélanges de métaux et de biomatériaux
• B33Y 80/00 - Produits obtenus par fabrication additive
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
• C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
• C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
• C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
• C12Q 1/00 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions

Abrégé

Aspects of pH responsive polymers are described. One exemplary aspect is a pH responsive GelMA polymer comprising a gelatin type A with selectively modified and unmodified functional groups. For example, this polymer may comprise: modified carboxyl groups, modified hydroxyl groups, unmodified carboxyl groups, unmodified hydroxyl groups, and unmodified amine groups; carboxyl and hydroxyl groups that have been methacrylated; or carboxyl, hydroxyl, and amine groups that were not methacrylated, wherein the modified and unmodified functional groups of Polymer A may be selected by controlling a pH level of its synthesizing reaction. Another exemplary aspect is a pH responsive GelMA polymer comprising a gelatin type B with selectively modified amine groups, modified hydroxyl groups, unmodified carboxyl groups, unmodified, hydroxyl groups, and unmodified amine groups. Related apparatus, compositions, methods, polymers, and systems are described.

Classes IPC  ?

• A61K 9/16 - AgglomérésGranulésMicrobilles
• A61K 33/242 - OrSes composés
• B01J 13/14 - Polymérisation, réticulation
• C08H 1/06 - Produits macromoléculaires dérivés des protéines dérivés de corne, sabots, cheveux, cuirs ou peaux
• C08L 89/06 - Produits dérivés de déchets, p. ex. corne, sabot, cheveux ou fils dérivés des cuirs ou des peaux

Abrégé

Bioinks and methods of synthesizing, characterizing, and deploying bioinks are described. Compounds and methods of making the same are described. Method of synthesizing pH-responsive polymers are described. Different types of bioinks are described. For example, the described bioinks may comprise: one or more of the pH-responsive polymers; a photoinitiator; and a solvent. Methods preparing bioinks are described. Methods of determining characteristics of bioinks are described. Related apparatus, kits, methods, and systems also are described.

Classes IPC  ?

• C08H 1/00 - Produits macromoléculaires dérivés des protéines
• B33Y 70/00 - Matériaux spécialement adaptés à la fabrication additive
• C08B 37/08 - ChitineSulfate de chondroïtineAcide hyaluroniqueLeurs dérivés
• C08J 3/075 - Gels macromoléculaires
• C08L 5/08 - ChitineSulfate de chondroïtineAcide hyaluroniqueLeurs dérivés
• C08L 89/00 - Compositions contenant des protéinesCompositions contenant leurs dérivés
• C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
• G01N 3/00 - Recherche des propriétés mécaniques des matériaux solides par application d'une contrainte mécanique
• G01N 3/42 - Recherche de la dureté ou de la dureté au rebondissement en effectuant des empreintes sous une charge permanente par des dispositifs de pénétration, p. ex. sphère, pyramide

Abrégé

Aspects of pH responsive polymers are described. One exemplary aspect is a pH responsive GelMA polymer comprising a gelatin type A with selectively modified and unmodified functional groups. For example, this polymer may comprise: modified carboxyl groups, modified hydroxyl groups, unmodified carboxyl groups, unmodified hydroxyl groups, and unmodified amine groups; carboxyl and hydroxyl groups that have been methacrylated; or carboxyl, hydroxyl, and amine groups that were not methacrylated, wherein the modified and unmodified functional groups of Polymer A may be selected by controlling a pH level of its synthesizing reaction. Another exemplary aspect is a pH responsive GelMA polymer comprising a gelatin type B with selectively modified amine groups, modified hydroxyl groups, unmodified carboxyl groups, unmodified, hydroxyl groups, and unmodified amine groups. Related apparatus, compositions, methods, polymers, and systems are described.

Classes IPC  ?

• C08H 1/06 - Produits macromoléculaires dérivés des protéines dérivés de corne, sabots, cheveux, cuirs ou peaux
• C08J 3/075 - Gels macromoléculaires
• C08L 89/06 - Produits dérivés de déchets, p. ex. corne, sabot, cheveux ou fils dérivés des cuirs ou des peaux

Abrégé

Aspects of pH responsive polymers are described. One exemplary aspect is a pH responsive GelMA polymer comprising a gelatin type A with selectively modified and unmodified functional groups. For example, this polymer may comprise: modified carboxyl groups, modified hydroxyl groups, unmodified carboxyl groups, unmodified hydroxyl groups, and unmodified amine groups; carboxyl and hydroxyl groups that have been methacrylated; or carboxyl, hydroxyl, and amine groups that were not methacrylated, wherein the modified and unmodified functional groups of Polymer A may be selected by controlling a pH level of its synthesizing reaction. Another exemplary aspect is a pH responsive GelMA polymer comprising a gelatin type B with selectively modified amine groups, modified hydroxyl groups, unmodified carboxyl groups, unmodified, hydroxyl groups, and unmodified amine groups. Related apparatus, compositions, methods, polymers, and systems are described.

Classes IPC  ?

• C08H 1/06 - Produits macromoléculaires dérivés des protéines dérivés de corne, sabots, cheveux, cuirs ou peaux
• C08J 3/24 - Réticulation, p. ex. vulcanisation, de macromolécules
• C08J 3/075 - Gels macromoléculaires
• C08L 89/06 - Produits dérivés de déchets, p. ex. corne, sabot, cheveux ou fils dérivés des cuirs ou des peaux

Produits et services

Biologically compatible materials, namely, hydrogels, polymers, and related materials used for 3D bioprinting tissue models, vascular structures, scaffolds, polymeric networks, and/or biological tissue specimens capable of mimicking different types of tissues for scientific use. Pharmaceutical preparations and screening, namely, hydrogels, polymers, and related preparations and materials used for 3D bioprinting tissue models, vascular structures, scaffolds, polymeric networks, and/or biological tissue specimens capable of mimicking different types of tissues for medical use.

Produits et services

(1) Biologically compatible materials, namely hydrogels, polymers, and related materials used for 3D tissue models, vascular structures, scaffolds, polymeric networks, and/or biological tissue specimens capable of mimicking different types of human tissues for medical use and/or scientific research; biological tissue cultures for medical purposes; tissue-regenerative pharmaceutical preparations; and biological products made from such material. (2) Pharmaceutical preparations and screening, namely hydrogels, polymers, and related preparations used for 3D bioprinting tissue models, vascular structures, scaffolds, polymeric networks, and/or biological tissue specimens capable of mimicking different types of human tissues for medical and scientific use; biological tissue cultures for medical purposes; tissue-regenerative pharmaceutical preparations; and pharmaceutical products made with such preparations. (1) Scientific research; biological research; scientific and technological consulting services and research and design relating thereto in the fields of cell biology and life science research; research, design and development of tools for tissue testing; research, design and development of instruments and devices for cell research; research, design and development for instruments and devices for 3D bioprinting; research, design and development of tools for 3D printing of biologic products, biological tissues and proteins.

Produits et services

(1) Biologically compatible materials, namely, hydrogels, polymers, and related materials used for 3D bioprinting tissue models, vascular structures, scaffolds, polymeric networks, and/or biological tissue specimens capable of mimicking different types of tissues for scientific use. (2) Pharmaceutical preparations and screening, namely, hydrogels, polymers, and related preparations and materials used for 3D bioprinting tissue models, vascular structures, scaffolds, polymeric networks, and/or biological tissue specimens capable of mimicking different types of tissues for medical use.

Produits et services

Biologically compatible materials, namely, hydrogels used to create polymeric networks for three-dimensional (3D) tissue models, vascular structures, scaffolds, and biological tissue specimens capable of mimicking different types of human tissues for medical use and scientific research; Biologically compatible materials, namely, polymers for use in three-dimensional (3D) tissue models, vascular structures, scaffolds, polymeric networks, and biological tissue specimens capable of mimicking different types of human tissues for medical use and scientific research; biological products for non-medical use, namely, reagents for scientific and medical research use Pharmaceutical preparations namely, a three-dimensional (3D) bioprinted drug delivery system comprised of hydrogel-based and polymer-based bioinks for the creation of biological tissues for medical purposes; biological tissue cultures for medical purposes; tissue-regenerative pharmaceutical preparations Scientific research; biological research; scientific and technological consulting services in the cell biology and life sciences industry, and research and design relating thereto; research, design and development of tools for tissue testing; research, design and development of instruments and devices for stem cell research; research, design and development of instruments and devices for three-dimensional (3D) bioprinting; research, design and development of tools for three-dimensional (3D)printing of biological products, biological tissues and proteins; Drug screening for medical purposes Drug screening for scientific research purposes

Produits et services

Biologically compatible materials for three-dimensional (3D) bioprinted tissue models, namely, hydrogels and polymers used to create vascular structures, scaffolds, polymeric networks, and biological tissue specimens capable of mimicking different types of tissues for medical and scientific purposes Pharmaceutical preparations, namely, three-dimensional (3D) bioprinted drug delivery systems comprised of hydrogel-based medical dressings and polymer-based systems for the continuous release of a wide variety of therapeutic agents