This disclosure describes methods for growing cells on chickpea microcarriers as part of a process of creating a comestible food product. Generally, the disclosed method comprises adding chickpea microcarriers to cell culture media in a vessel. The chickpea microcarriers are mixed with non-human cells in the cell culture media. The chickpea microcarriers and non-human cells are mixed to adhere and grow the non-human cells on the surface of the chickpea microcarriers. After mixing, the non-human cells adhered to the surface of the chickpea microcarriers forms a textured cell tissue. The textured cell tissue can be harvested from the vessel and formed into a comestible food product.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
A23J 3/22 - Traitement des protéines pour l'alimentation par texturation
A23L 11/00 - Légumes secs, c. à d. fruits de plantes légumineuses, pour l'obtention d'aliments; Produits dérivés de légumineuses; Leur préparation ou leur traitement
This disclosure describes methods for growing cells in a controlled environment in a growing room, transporting the cells to a harvest room, and removing the cells from the controlled environment in the harvest room. The disclosed method can limit exposure of cells to contaminants by maintaining a controlled environment through cell growth and additional processing. For instance, the cells may be washed and cooled within the controlled environment. The cells are transferred to a harvest room where they are harvested and removed from the controlled environment.
This disclosure describes methods for forming cell-based-meat products that mimic cuts of slaughtered meat. Generally, the disclosed method comprises forming a cell mass into primary structures, including fibers and/or sheets. A toughening agent is applied to the exterior surfaces of the primary structures, and the primary structures are arranged to mimic structures in a target slaughtered meat. The toughening agent creates a textural contrast that, during consumption, is like the textural contrast experienced when biting through bundles of meat fibers in slaughtered meat.
The present disclosure relates to systems, apparatuses, and methods for preparing cell-based meat products (i.e., comestible meat products). In particular, a pipe-based bioreactor is disclosed having one or more substrates disposed therein. In one or more embodiments, the one or more substrates comprise a plurality of nominally spaced substrates conforming to an interior profile of an elongated enclosure of the pipe-based bioreactor. In some embodiments, multiple pipe-based bioreactors are interconnected a fluid source for preparing cell-based meat products. In addition, various methods and procedures for utilizing embodiments of pipe-based bioreactors are disclosed.
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
A22C 17/00 - Autres dispositifs pour le traitement de la viande ou des os
A23L 13/00 - Produits à base de viande; Farine de viande; Leur préparation et leur traitement
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 3/04 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus comportant des moyens fournissant des couches minces
6.
COMPOSITIONS AND METHODS OF MAKING COMESTIBLE CELL-BASED MEAT FILLETS
Provided herein are methods of making a comestible cell-based meat composition where the method includes cooling the cell mass from a growth temperature to a temperature below a growth temperature; harvesting at least a portion of the cell mass; de-wetting the harvested cell mass with mechanically-generated pressure; forming the cell-based meat composition into a fillet; and optionally processing the fillet under high pressure, until the comestible cell-based meat composition satisfies a texture profile criteria.
Provided herein are of making a comestible cell-based meat composition where the method includes cooling the cell mass from a growth temperature to a temperature below a growth temperature; harvesting at least a portion of the cell mass; de-wetting the harvested cell mass with mechanically-generated pressure; forming the cell-based meat composition into a fillet; and processing the fillet under high pressure until the comestible cell-based meat composition satisfies a texture profile criteria.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
A23J 3/22 - Traitement des protéines pour l'alimentation par texturation
8.
SUBSTRATE APPARATUS WITH MULTI-LAYER SUBSTRATE FOR CELL-BASED MEAT CULTIVATORS
The present disclosure relates to a substrate apparatus (and methods of manufacturing the same) with one or more substrates having a substrate spacing for growing a cell mass. In particular embodiments, the disclosed substrate apparatus includes a substrate wound into a coiled configuration with an intra-coil spacing between coil layers. To provide the intra-coil spacing, certain implementations use a separator. For example, a separator is applied to a substrate surface, and the separator-substrate combination is wound together (e.g., around a spool). In turn, a locking element is attached to the substrate to maintain the coiled configuration. The separator is then removed via heat treatment, chemical treatment, or physical displacement-thereby leaving the intra-coil spacing between the coil layers. Alternatively, no separator is used to provide a substrate spacing. For example, in lieu of a separator, the locking element is actively applied to the substrate during the winding process.
Provided herein are methods of method for extending the replicative capacity of a bovine cell. For example, a method for extending the replicative capacity of a bovine cell includes (a) isolating a population of cells from bovine tissue; (b) introducing into a cell of the population of cells a polynucleotide encoding telomere reverse transcriptase (TERT); (c) introducing into the cell of step (b) at least a first polynucleotide encoding a first protein that is capable of modulating cell proliferation; and (d) culturing the population of bovine cells in a cultivation infrastructure is provided herein.
Provided herein are methods of method for extending the replicative capacity of a bovine cell. For example, a method for extending the replicative capacity of a bovine cell includes (a) isolating a population of cells from bovine tissue; (b) introducing into a cell of the population of cells a polynucleotide encoding telomere reverse transcriptase (TERT); (c) introducing into the cell of step (b) at least a first polynucleotide encoding a first protein that is capable of modulating cell proliferation; and (d) culturing the population of bovine cells in a cultivation infrastructure is provided herein..
A23L 13/74 - Morceaux de viande attendris ou aromatisés; Solutions de macération ou de marinage spécialement adaptées à cet effet en utilisant des additifs, p. ex. par injection de solutions en utilisant des micro-organismes ou des enzymes
11.
APPARATUSES AND SYSTEMS FOR PREPARING A MEAT PRODUCT
The apparatuses described herein relate to preparation of a meat product. Apparatuses, systems comprising the apparatuses, and methods of making and use the systems and apparatuses are described herein. These are useful for controlling one or more of growth on and separation of a meat product from an enclosed substrate. The apparatuses and systems are configured to receive fluid and grow the meat product and/or separate the meat product from the substrate in a scalable manner.
Provided herein are cell-based meat compositions suitable for consumption comprising cells harvested from suspension cell culture and comprising an emulsion mixture. Also provide here are methods of making a cell-based meat composition suitable for consumption comprising: culturing a population of cells in suspension; harvesting the population of cells to produce a cell mass; formulating an emulsion mixture by: (i) emulsifying an oil with a hydrocolloid to produce an oil:hydrocolloid mixture; and (ii) adding the cell mass to the oil:hydrocolloid mixture to form an emulsion mixture; and (d) forming the cell-based meat composition into a patty.
A23J 3/22 - Traitement des protéines pour l'alimentation par texturation
A23L 29/10 - Aliments ou produits alimentaires contenant des additifs; Leur préparation ou leur traitement contenant des émulsifiants
A23L 29/238 - Aliments ou produits alimentaires contenant des additifs; Leur préparation ou leur traitement contenant des agents gélifiants ou épaississants d'origine végétale à partir de graines, p.ex. gomme de caroube ou gomme de guar
13.
METHODS FOR CULTIVATING TISSUE ON POROUS SUBSTRATES
Described herein are novel systems and methods for biomanufacturing, such as tissue cultivation. In some variations the systems and methods may comprise at least one porous substrate.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p.ex. magnétisme, ondes sonores
14.
PIPE-BASED BIOREACTORS FOR PRODUCING COMESTIBLE MEAT PRODUCTS AND METHODS OF USING THE SAME
The present disclosure relates to systems, apparatuses, and methods for preparing cell-based meat products (i.e., comestible meat products). In particular, a pipe-based bioreactor is disclosed having one or more substrates disposed therein. In one or more embodiments, the one or more substrates comprise a plurality of nominally spaced substrates conforming to an interior profile of an elongated enclosure of the pipe-based bioreactor. In some embodiments, multiple pipe-based bioreactors are interconnected a fluid source for preparing cell-based meat products. In addition, various methods and procedures for utilizing embodiments of pipe-based bioreactors are disclosed.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
This disclosure describes methods for forming cell-based-meat products that mimic cuts of slaughtered meat. Generally, the disclosed method comprises forming a cell mass into primary structures, including fibers and/or sheets. A toughening agent is applied to the exterior surfaces of the primary structures, and the primary structures are arranged to mimic structures in a target slaughtered meat. The toughening agent creates a textural contrast that, during consumption, is like the textural contrast experienced when biting through bundles of meat fibers in slaughtered meat.
A23L 29/00 - Aliments ou produits alimentaires contenant des additifs; Leur préparation ou leur traitement
A23L 5/00 - Préparation ou traitement des aliments ou produits alimentaires en général; Aliments ou produits alimentaires ainsi obtenus; Leurs matériaux
A23J 3/26 - Traitement des protéines pour l'alimentation par texturation utilisant l'extrusion ou l'expansion
A23J 3/22 - Traitement des protéines pour l'alimentation par texturation
A23J 3/28 - Traitement des protéines pour l'alimentation par texturation utilisant la coagulation à partir de ou dans un bain, p.ex. fibres filées
This disclosure describes methods for growing cells in a controlled environment in a growing room, transporting the cells to a harvest room, and removing the cells from the controlled environment in the harvest room. The disclosed method can limit exposure of cells to contaminants by maintaining a controlled environment through cell growth and additional processing. For instance, the cells may be washed and cooled within the controlled environment. The cells are transferred to a harvest room where they are harvested and removed from the controlled environment.
The present disclosure relates to systems, apparatuses, and methods for preparing cell-based meat products (i.e., comestible meat products). In particular, a pipe-based bioreactor is disclosed having one or more substrates disposed therein. In one or more embodiments, the one or more substrates comprise a plurality of nominally spaced substrates conforming to an interior profile of an elongated enclosure of the pipe-based bioreactor. In some embodiments, multiple pipe-based bioreactors are interconnected a fluid source for preparing cell-based meat products. In addition, various methods and procedures for utilizing embodiments of pipe-based bioreactors are disclosed.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
A22C 17/00 - Autres dispositifs pour le traitement de la viande ou des os
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 3/04 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus comportant des moyens fournissant des couches minces
A23L 13/00 - Produits à base de viande; Farine de viande; Leur préparation et leur traitement
18.
Methods of using pipe-based bioreactors for producing comestible meat products
The present disclosure relates to systems, apparatuses, and methods for preparing cell-based meat products (i.e., comestible meat products). In particular, a pipe-based bioreactor is disclosed having one or more substrates disposed therein. In one or more embodiments, the one or more substrates comprise a plurality of nominally spaced substrates conforming to an interior profile of an elongated enclosure of the pipe-based bioreactor. In some embodiments, multiple pipe-based bioreactors are interconnected a fluid source for preparing cell-based meat products. In addition, various methods and procedures for utilizing embodiments of pipe-based bioreactors are disclosed.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
A22C 17/00 - Autres dispositifs pour le traitement de la viande ou des os
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 3/04 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus comportant des moyens fournissant des couches minces
A23L 13/00 - Produits à base de viande; Farine de viande; Leur préparation et leur traitement
19.
CELL-BASED MEAT PRODUCTS AND CELL BIOMASSES WITH CELLS CULTIVATED IN A FILTER CAKE
Provided herein are filter cake-based systems and methods for cultivating cells and cell biomass therefrom. Provided herein is a system for cultivating cells and cell biomass comprising a filter chamber comprising at least one inlet and at least one outlet, at least one filter support located within the filter chamber, and a filter cake located on the filter support, wherein the filter cake comprises at least one filter aid and a plurality of cells. Provided herein is a method for optimizing the cultivation of cells and cell biomass, comprising providing a filter support, adding at least one filter aid to the filter support, adding a plurality of cells to the filter aid, wherein the cells and the filter aid together comprise a filter cake, growing the cells into a cell biomass in the filter cake, wherein the filter cake is at least partially compressible.
This disclosure describes methods for forming cell-based-meat products that mimic cuts of slaughtered meat. Generally, the disclosed method comprises forming a cell mass into primary structures, including fibers and/or sheets. A toughening agent is applied to the exterior surfaces of the primary structures, and the primary structures are arranged to mimic structures in a target slaughtered meat. The toughening agent creates a textural contrast that, during consumption, is like the textural contrast experienced when biting through bundles of meat fibers in slaughtered meat.
This disclosure describes methods for growing cells in a controlled environment in a growing room, transporting the cells to a harvest room, and removing the cells from the controlled environment in the harvest room. The disclosed method can limit exposure of cells to contaminants by maintaining a controlled environment through cell growth and additional processing. For instance, the cells may be washed and cooled within the controlled environment. The cells are transferred to a harvest room where they are harvested and removed from the controlled environment.
A23L 13/00 - Produits à base de viande; Farine de viande; Leur préparation et leur traitement
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
22.
ELECTRICAL FEED CONNECTOR SYSTEM AND METHOD FOR LIGHT EMITTING DIODE (LED) CIRCUIT BOARDS WITH VARIED PLACEMENT OF WIRE TERMINAL CONNECTION POINTS
A system comprises a printed circuit board (PCB) substrate and a housing. The PCB substrate has a plurality of light emitting diodes (LEDs), a first plurality of electrical conductor portions and a second plurality of electrical conductor portions. The housing has a first side and a second side opposite the first side. The housing has on the first side a first wire terminal and a second wire terminal. The housing has on the second side a first conductor contact and a second conductor contact. The housing is configured to be attachable to the PCB substrate so that first conductor contact is electrically coupled to an electrical conductor portion from the first plurality of electrical conductor portions and the second conductor contact is electrically coupled to an electrical conductor portion from the second plurality of electrical conductor portions.
H01R 12/63 - Connexions fixes pour circuits imprimés flexibles, câbles plats ou à rubans ou structures similaires se raccordant à des câbles d'une autre forme
H01R 12/51 - Connexions fixes pour circuits imprimés rigides ou structures similaires
H05K 1/18 - Circuits imprimés associés structurellement à des composants électriques non imprimés
H05K 3/32 - Connexions électriques des composants électriques ou des fils à des circuits imprimés
This disclosure describes methods for growing cells in a controlled environment in a growing room, transporting the cells to a harvest room, and removing the cells from the controlled environment in the harvest room. The disclosed method can limit exposure of cells to contaminants by maintaining a controlled environment through cell growth and additional processing. For instance, the cells may be washed and cooled within the controlled environment. The cells are transferred to a harvest room where they are harvested and removed from the controlled environment.
This disclosure describes methods and apparatuses for creating or using a fibrous cell substrate that is formed in a mold using temperature and wettability gradients and that upon which cells can be grown. The disclosed method can include utilizing a temperature gradient and a wettability gradient within a mold to form a porous cell substrate. In particular, the temperature and wettability gradients control nucleation and growth of crystals within the cell substrate solution to form parallel layers of cell substrate. The crystals can be sublimated by freeze drying and the porous cell substrate is seeded. More specifically, the disclosed method includes using pressure and an increased cell mixture viscosity to seed cells deep within the cell substrate. This disclosure also describes an apparatus for forming the structured porous cell substrate.
This disclosure describes methods and apparatuses for creating or using a fibrous cell substrate that is formed in a mold using temperature and wettability gradients and that upon which cells can be grown. The disclosed method can include utilizing a temperature gradient and a wettability gradient within a mold to form a porous cell substrate. In particular, the temperature and wettability gradients control nucleation and growth of crystals within the cell substrate solution to form parallel layers of cell substrate. The crystals can be sublimated by freeze drying and the porous cell substrate is seeded. More specifically, the disclosed method includes using pressure and an increased cell mixture viscosity to seed cells deep within the cell substrate. This disclosure also describes an apparatus for forming the structured porous cell substrate.
C12M 3/04 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus comportant des moyens fournissant des couches minces
Provided herein are methods relating to making non-natively occurring myogenic cells. For example, provided herein are methods for transdifferentiating a liver-derived cell to a non- natively occurring myogenic cell. In another example, the method relates to using dedifferentiation to make non-natively occurring myogenic cells.
Provided herein are cell-based meat compositions suitable for consumption comprising cells harvested from suspension cell culture and comprising an emulsion mixture. Also provide here are methods of making a cell-based meat composition suitable for consumption comprising: culturing a population of cells in suspension; harvesting the population of cells to produce a cell mass; formulating an emulsion mixture by: (i) emulsifying an oil with a hydrocolloid to produce an oil:hydrocolloid mixture; and (ii) adding the cell mass to the oil:hydrocolloid mixture to form an emulsion mixture; and (d) forming the cell-based meat composition into a patty.
Provided herein are cell-based meat compositions suitable for consumption comprising cells harvested from suspension cell culture and comprising an emulsion mixture. Also provide here are methods of making a cell-based meat composition suitable for consumption comprising: culturing a population of cells in suspension; harvesting the population of cells to produce a cell mass; formulating an emulsion mixture by: (i) emulsifying an oil with a hydrocolloid to produce an oil:hydrocolloid mixture; and (ii) adding the cell mass to the oil:hydrocolloid mixture to form an emulsion mixture; and (d) forming the cell-based meat composition into a patty.
A23J 3/22 - Traitement des protéines pour l'alimentation par texturation
A23L 29/238 - Aliments ou produits alimentaires contenant des additifs; Leur préparation ou leur traitement contenant des agents gélifiants ou épaississants d'origine végétale à partir de graines, p.ex. gomme de caroube ou gomme de guar
A23L 29/10 - Aliments ou produits alimentaires contenant des additifs; Leur préparation ou leur traitement contenant des émulsifiants
29.
NON-SKELETAL MUSCLE-DERIVED CELLS AS A SOURCE OF SUSPENSION CAPABLE MYOGENIC CELLS FOR CULTURED FOODS
Provided herein are methods relating to making non-natively occurring myogenic cells. For example, provided herein are methods for transdifferentiating a liver-derived cell to a non-natively occurring myogenic cell. In another example, the method relates to using dedifferentiation to make non-natively occurring myogenic cells.
Provided herein are methods of co-culturing a myogenic cell with a support cell to increase myotube formation from the myogenic cells. For example, provided herein is a method comprising co-culturing a myogenic cell with a support cell wherein the support cell comprises a polynucleotide comprising a coding sequence of a gene of interest; and culturing the myogenic cell and the support cell in a cultivation infrastructure under conditions sufficient to induce proliferation of the myoblast cell.
Provided herein are methods of engineering a cell line for reduced dependence on exogenous growth factor(s). In some embodiments, the method includes introducing into a cell one or more of: a polynucleotide comprising a coding sequence of a growth factor ligand; a polynucleotide comprising a coding sequence of a growth factor receptor; or a polynucleotide comprising a coding sequence of an activated downstream growth factor target and culturing the cells in a cultivation infrastructure.
Provided herein are methods of engineering a cell line for reduced dependence on exogenous growth factor(s). In some embodiments, the method includes introducing into a cell one or more of: a polynucleotide comprising a coding sequence of a growth factor ligand; a polynucleotide comprising a coding sequence of a growth factor receptor; or a polynucleotide comprising a coding sequence of an activated downstream growth factor target, and culturing the cells in a cultivation infrastructure.
This disclosure relates to methods of shaping a raw cell-based-meat product using vacuum sealing. The disclosed method includes preparing a cell-mass mixture by mixing a grown cell mass with a binding agent. The cell-mass mixture is added to a mold that is covered with a film and vacuum sealed. Because, in some embodiments, the mold is rigid, the cell-mass mixture conforms to the shape of the mold, such as the shape of slaughtered or harvested meat. To shape a cell-based-meat product, an apparatus may be used with a mold for sealing and conforming a cell-mass mixture.
C12N 5/071 - Cellules ou tissus de vertébrés, p.ex. cellules humaines ou tissus humains
A23L 13/00 - Produits à base de viande; Farine de viande; Leur préparation et leur traitement
A23L 3/3418 - Conservation des aliments ou produits alimentaires, en général, p.ex. pasteurisation ou stérilisation, spécialement adaptée aux aliments ou produits alimentaires par traitement au moyen de produits chimiques sous forme de gaz, p.ex. fumigation; Compositions ou appareils à cet effet en atmosphère contrôlée, p.ex. sous vide partiel, comportant uniquement les gaz suivants: CO2, N2, O2 ou H2O
34.
VACUUM DRYING A COMESTIBLE CELL-BASED MEAT PRODUCT
The present disclosure relates to one or more vacuum drying methods and apparatuses to partially dry a comestible cell-based meat product after being harvested from a bioreactor. The vacuum drying method includes implementing a pressure-temperature environment to evaporate a portion of the moisture content from a comestible cell-based meat product. In doing so, the vacuum drying method can concentrate protein in the comestible cell-based meat product, as well as minerals and other non-liquid components. Specifically, at refrigeration temperatures, the vacuum drying method preserves cellular structure of the comestible cell-based meat product and facilitates safe processing according to various rules and regulations for raw meat sale, distribution, and consumer consumption. In addition, lowered environmental pressure provides reduced drying times to achieve a particular moisture content criteria or threshold mass of the comestible cell-based meat product. Once partially dried, the comestible cell-based meat product can be subsequently processed for raw meat packaging.
The present disclosure relates to one or more vacuum drying methods and apparatuses to partially dry a comestible cell-based meat product after being harvested from a bioreactor. The vacuum drying method includes implementing a pressure-temperature environment to evaporate a portion of the moisture content from a comestible cell-based meat product. In doing so, the vacuum drying method can concentrate protein in the comestible cell-based meat product, as well as minerals and other non-liquid components. Specifically, at refrigeration temperatures, the vacuum drying method preserves cellular structure of the comestible cell-based meat product and facilitates safe processing according to various rules and regulations for raw meat sale, distribution, and consumer consumption. In addition, lowered environmental pressure provides reduced drying times to achieve a particular moisture content criteria or threshold mass of the comestible cell-based meat product. Once partially dried, the comestible cell-based meat product can be subsequently processed for raw meat packaging.
The present disclosure relates to a substrate apparatus (and methods of manufacturing the same) with one or more substrates having a substrate spacing for growing a cell mass. In particular embodiments, the disclosed substrate apparatus includes a substrate wound into a coiled configuration with an intra-coil spacing between coil layers. To provide the intra-coil spacing, certain implementations use a separator. For example, a separator is applied to a substrate surface, and the separator-substrate combination is wound together (e.g., around a spool). In turn, a locking element is attached to the substrate to maintain the coiled configuration. The separator is then removed via heat treatment, chemical treatment, or physical displacement—thereby leaving the intra-coil spacing between the coil layers. Alternatively, no separator is used to provide a substrate spacing. For example, in lieu of a separator, the locking element is actively applied to the substrate during the winding process.
Provided herein are methods to increase the culture density and/or thickness of a cellular biomass in a cultivation infrastructure, to improve the culture of cells in the absence of serum in a cultivation infrastructure, and to promote anchorage-independent growth of a cellular biomass in a cultivation infrastructure. The methods comprise inhibiting the HIPPO signaling pathway, for example, by activating YAP1, activating TAZ, and/or inhibiting MOB1, LATS1 kinase, LATS2 kinase, WW45, MST1 kinase, and/or MST2 kinase in the cellular biomass. In some embodiments, the cellular biomass is harvested from the cultivation infrastructure for the formulation of cell-based food products or ingredients, such as animal meat manufactured from cells in an ex vivo process or for therapeutic applications such as organ or tissue transplantation or grafting.
C12N 5/077 - Cellules mésenchymateuses, p.ex. cellules osseuses, cellules de cartilage, cellules stromales médulaires, cellules adipeuses ou cellules musculaires
C07K 14/47 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés provenant d'humains provenant de vertébrés provenant de mammifères
38.
APPARATUSES AND SYSTEMS FOR PREPARING A MEAT PRODUCT
The apparatuses described herein relate to preparation of a cell-based meat product. Apparatuses, systems comprising the apparatuses, and methods of making and use the systems and apparatuses are described herein. These are useful for controlling one or more of growth on and separation of a meat product from an enclosed substrate. The apparatuses and systems are configured to receive fluid and grow the meat product and/or separate the meat product from the substrate in a scalable manner.
A23J 3/20 - Protéines à partir de micro-organismes ou à partir d'algues unicellulaires
C12M 3/04 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus comportant des moyens fournissant des couches minces
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
39.
APPARATUSES AND SYSTEMS FOR PREPARING A MEAT PRODUCT
The apparatuses described herein relate to preparation of a cell-based meat product. Apparatuses, systems comprising the apparatuses, and methods of making and use the systems and apparatuses are described herein. These are useful for controlling one or more of growth on and separation of a meat product from an enclosed substrate. The apparatuses and systems are configured to receive fluid and grow the meat product and/or separate the meat product from the substrate in a scalable manner.
A23J 3/20 - Protéines à partir de micro-organismes ou à partir d'algues unicellulaires
C12M 3/04 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus comportant des moyens fournissant des couches minces
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
40.
SCALING UP MYOGENIC TISSUE: LATE PASSAGE MYOGENICITY
The present disclosure relates to methods for improving myogenic differentiation capacity of a cell line or an immortalized cell line. For example, the present disclosure relates to methods of exposing an immortalized cell line (e.g., an immortalized fibroblast cell line) to culture media comprising signaling pathway agonists, antagonist, or a combination thereof in order to improve differentiation capacity. In another example, the present disclosure relates to methods of improving differentiation capacity of a cell line or an immortalized cell line where the method includes transforming an immortalized cell line with one or more myogenic regulatory factors and exposing the immortalized cell line to culture media comprising signaling pathway agonists, antagonists, or a combination thereof.
This disclosure relates to methods of washing cells from a grown cell mass to remove cell culture media and enriching the cells with a finishing media. The disclosed method includes growing a cell mass in cell culture media and then collecting and washing the grown cell mass with a series of wash buffers or a gradient wash buffer that changes over time. While the cell culture media contains nutrients and components beneficial for cell growth, cells grown in cell culture media often have off flavors, off aromas, poor color, poor salt/minerality compositions, and other shortcomings. Accordingly, the disclosed method comprises removing cell culture media remnants from a grown cell mass using a single or a series of wash media. The grown cell mass is further washed with a finishing or enrichment buffer to further improve sensory aspects and nutritional composition of the grown cell mass.
The present disclosure relates to methods for improving myogenic differentiation capacity of a cell line or an immortalized cell line. For example, the present disclosure relates to methods of exposing an immortalized cell line (e.g., an immortalized fibroblast cell line) to culture media comprising signaling pathway agonists, antagonist, or a combination thereof in order to improve differentiation capacity. In another example, the present disclosure relates to methods of improving differentiation capacity of a cell line or an immortalized cell line where the method includes transforming an immortalized cell line with one or more myogenic regulatory factors and exposing the immortalized cell line to culture media comprising signaling pathway agonists, antagonists, or a combination thereof.
C07K 14/47 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés provenant d'humains provenant de vertébrés provenant de mammifères
C12N 5/077 - Cellules mésenchymateuses, p.ex. cellules osseuses, cellules de cartilage, cellules stromales médulaires, cellules adipeuses ou cellules musculaires
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p.ex. kinases (2.7)
C12N 15/87 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p.ex. co-transformation
43.
METHOD FOR PRESSURIZING CELLS GROWN IN HYDROGEL TO INDUCE HYPERTROPHY
This disclosure relates to methods of growing cells within a hydrogel scaffold and pressurizing the hydrogel and cells to induce the cells to stretch and differentiation. The disclosed method can include coating a substrate of a bioreactor with a hydrogel and seeding cells onto the hydrogel and/or the substrate. The disclosed method can further include growing the seeded cells into a cell mass and pressurizing the cell mass and the hydrogel within the bioreactor. Pressurizing the cell mass and the hydrogel induces the cell mass and hydrogel to mechanically stretch, thereby inducing hypertrophy and cell alignment.
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
44.
METHOD FOR WASHING AND FINISHING A GROWN CELL MASS
This disclosure relates to methods of washing cells from a grown cell mass to remove cell culture media and enriching the cells with a finishing media. The disclosed method includes growing a cell mass in cell culture media and then collecting and washing the grown cell mass with a series of wash buffers or a gradient wash buffer that changes over time. While the cell culture media contains nutrients and components beneficial for cell growth, cells grown in cell culture media often have off flavors, off aromas, poor color, poor salt/minerality compositions, and other shortcomings. Accordingly, the disclosed method comprises removing cell culture media remnants from a grown cell mass using a single or a series of wash media. The grown cell mass is further washed with a finishing or enrichment buffer to further improve sensory aspects and nutritional composition of the grown cell mass.
C12N 5/077 - Cellules mésenchymateuses, p.ex. cellules osseuses, cellules de cartilage, cellules stromales médulaires, cellules adipeuses ou cellules musculaires
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
A23L 13/00 - Produits à base de viande; Farine de viande; Leur préparation et leur traitement
45.
SCALING UP MYOGENIC TISSUE: LATE PASSAGE MYOGENICITY
The present disclosure relates to methods for improving myogenic differentiation capacity of a cell line or an immortalized cell line. For example, the present disclosure relates to methods of exposing an immortalized cell line (e.g., an immortalized fibroblast cell line) to culture media comprising signaling pathway agonists, antagonist, or a combination thereof in order to improve differentiation capacity. In another example, the present disclosure relates to methods of improving differentiation capacity of a cell line or an immortalized cell line where the method includes transforming an immortalized cell line with one or more myogenic regulatory factors and exposing the immortalized cell line to culture media comprising signaling pathway agonists, antagonists, or a combination thereof.
C12N 5/077 - Cellules mésenchymateuses, p.ex. cellules osseuses, cellules de cartilage, cellules stromales médulaires, cellules adipeuses ou cellules musculaires
C07K 14/47 - Peptides ayant plus de 20 amino-acides; Gastrines; Somatostatines; Mélanotropines; Leurs dérivés provenant d'humains provenant de vertébrés provenant de mammifères
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p.ex. kinases (2.7)
C12N 15/87 - Introduction de matériel génétique étranger utilisant des procédés non prévus ailleurs, p.ex. co-transformation
Meat substitute recipes with animal cells and exogenous heme-containing protein on plant-based meat dough matrices are disclosed. Methods of producing are also disclosed.
This disclosure relates to methods of size reducing, mixing, and folding subsets of a raw cell-based-meat product to improve the texture of a cell-based-meat product. The disclosed method can include collecting different portions of raw tissue product from either a single grown cell mass or different grown cell masses. The disclosed method can include finely chopping a first portion of cell mass into a fibrous pulp or paste and coarsely size-reducing a second portion of cell mass into segments. The first and second portions can be recombined and thoroughly mixed. In particular, the disclosed method includes mixing techniques that align fibers within the recombined portions. Examples of mixing techniques include dragging tines through the recombined portions, utilizing an extruder, and other methods. The combined mixture can further be flattened and folded to give the combined mixture additional structure and organization to better mimic conventional slaughtered meat.
This disclosure relates to methods of size reducing, mixing, and folding subsets of a raw cell-based-meat product to improve the texture of a cell-based-meat product. The disclosed method can include collecting different portions of raw tissue product from either a single grown cell mass or different grown cell masses. The disclosed method can include finely chopping a first portion of cell mass into a fibrous pulp or paste and coarsely size-reducing a second portion of cell mass into segments. The first and second portions can be recombined and thoroughly mixed. In particular, the disclosed method includes mixing techniques that align fibers within the recombined portions. Examples of mixing techniques include dragging tines through the recombined portions, utilizing an extruder, and other methods. The combined mixture can further be flattened and folded to give the combined mixture additional structure and organization to better mimic conventional slaughtered meat.
A23L 13/60 - Produits à base de viande hachée ou émulsifiée, p.ex. saucisses; Viande reconstituée à partir de viande hachée
A23L 13/77 - Morceaux de viande attendris ou aromatisés; Solutions de macération ou de marinage spécialement adaptées à cet effet par traitement mécanique, p. ex. par pétrissage, frottage ou barattage
49.
SUBSTRATE APPARATUS WITH MULTI-LAYER SUBSTRATE FOR CELL-BASED MEAT CULTIVATORS
The present disclosure relates to a substrate apparatus (and methods of manufacturing the same) with one or more substrates having a substrate spacing for growing a cell mass. In particular embodiments, the disclosed substrate apparatus includes a substrate wound into a coiled configuration with an intra-coil spacing between coil layers. To provide the intra-coil spacing, certain implementations use a separator. For example, a separator is applied to a substrate surface, and the separator-substrate combination is wound together (e.g., around a spool). In turn, a locking element is attached to the substrate to maintain the coiled configuration. The separator is then removed via heat treatment, chemical treatment, or physical displacement — thereby leaving the intra-coil spacing between the coil layers. Alternatively, no separator is used to provide a substrate spacing. For example, in lieu of a separator, the locking element is actively applied to the substrate during the winding process.
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
A23L 13/00 - Produits à base de viande; Farine de viande; Leur préparation et leur traitement
50.
Methods for the cultivation of cells and cell biomass in a filter cake
Provided herein are filter cake-based systems and methods for cultivating cells and cell biomass therefrom. Provided herein is a system for cultivating cells and cell biomass comprising a filter chamber comprising at least one inlet and at least one outlet, at least one filter support located within the filter chamber, and a filter cake located on the filter support, wherein the filter cake comprises at least one filter aid and a plurality of cells. Provided herein is a method for optimizing the cultivation of cells and cell biomass, comprising providing a filter support, adding at least one filter aid to the filter support, adding a plurality of cells to the filter aid, wherein the cells and the filter aid together comprise a filter cake, growing the cells into a cell biomass in the filter cake, wherein the filter cake is at least partially compressible.
Provided herein are filter cake-based systems and methods for cultivating cells and cell biomass therefrom. Provided herein is a system for cultivating cells and cell biomass comprising a filter chamber comprising at least one inlet and at least one outlet, at least one filter support located within the filter chamber, and a filter cake located on the filter support, wherein the filter cake comprises at least one filter aid and a plurality of cells. Provided herein is a method for optimizing the cultivation of cells and cell biomass, comprising providing a filter support, adding at least one filter aid to the filter support, adding a plurality of cells to the filter aid, wherein the cells and the filter aid together comprise a filter cake, growing the cells into a cell biomass in the filter cake, wherein the filter cake is at least partially compressible.
Provided herein are filter cake-based systems and methods for cultivating cells and cell biomass therefrom. Provided herein is a system for cultivating cells and cell biomass comprising a filter chamber comprising at least one inlet and at least one outlet, at least one filter support located within the filter chamber, and a filter cake located on the filter support, wherein the filter cake comprises at least one filter aid and a plurality of cells. Provided herein is a method for optimizing the cultivation of cells and cell biomass, comprising providing a filter support, adding at least one filter aid to the filter support, adding a plurality of cells to the filter aid, wherein the cells and the filter aid together comprise a filter cake, growing the cells into a cell biomass in the filter cake, wherein the filter cake is at least partially compressible.
53.
FILTER CAKE-BASED SYSTEMS AND METHODS FOR THE CULTIVATION OF CELLS AND CELL BIOMASS
Provided herein are filter cake-based systems and methods for cultivating cells and cell biomass therefrom. Provided herein is a system for cultivating cells and cell biomass comprising a filter chamber comprising at least one inlet and at least one outlet, at least one filter support located within the filter chamber, and a filter cake located on the filter support, wherein the filter cake comprises at least one filter aid and a plurality of cells. Provided herein is a method for optimizing the cultivation of cells and cell biomass, comprising providing a filter support, adding at least one filter aid to the filter support, adding a plurality of cells to the filter aid, wherein the cells and the filter aid together comprise a filter cake, growing the cells into a cell biomass in the filter cake, wherein the filter cake is at least partially compressible.
The substrates, systems, and methods described herein relate to textured substrates for preparing a comestible meat product. Substrates and methods are described herein for controlling one or more of growth, adhesion, retention, and/or release of cells (e.g., of a cell sheet) on or from the surface of the substrate. A method of preparing a comestible meat product may include applying a plurality of non-human cells to at least one patterned texture substrate, growing the cells on the patterned texture substrate to form the comestible meat product, and separating the comestible meat product from the patterned texture substrate. The patterned texture allows for improved growth, adhesion, retention, and/or release of cells as compared to another surface not comprising the patterned texture. In some embodiments, the cell culture substrate surfaces include a plurality of regions corresponding to a plurality of patterned textures.
Provided herein are compositions and methods to make and use engineered cells, for the purpose of increasing the cell density of a culture comprising metazoan cells and for the production of a cultured edible product.
Provided herein are compositions and methods to make and use engineered cells, for the purpose of increasing the cell density of a culture comprising metazoan cells and for the production of a cultured edible product.
A plant fat-based scaffold for growing cell-based meat products for consumption. The scaffold comprises primarily plant fats or waxes in addition to cell binding proteins and optional additional components that assist in the growth of cultivated animal cells. The scaffold can exist in both a liquified state during sterilization and a solid state during the formation of the scaffold, the seeding of the cultivated cells, and the cellular growth phase. The scaffold is capable of remaining in the final product for consumption or is partially or completely melted out of the final product and recycled into raw material for forming new scaffolds.
An assembly for delivering a fluid includes a fluid dispenser connected to a fluid supply conduit. The fluid dispenser includes a fluid outlet positioned along a length of the fluid dispenser. The fluid outlet is configured to deliver the fluid from the fluid supply conduit at a flow rate that varies along the length of the fluid dispenser.
B05B 3/10 - Appareillages de pulvérisation ou d'arrosage avec des éléments de sortie mobiles ou des éléments déflecteurs mobiles avec des éléments rotatifs avec évacuation sur la presque totalité de la périphérie de l'élément rotatif
B05B 3/04 - Appareillages de pulvérisation ou d'arrosage avec des éléments de sortie mobiles ou des éléments déflecteurs mobiles avec des éléments rotatifs entraînés par le liquide ou autre matériau fluide refoulé, p.ex. le liquide actionnant un moteur avant de parvenir à l'orifice de sortie
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
A plant fat-based scaffold for growing cell-based meat products for consumption. The scaffold comprises primarily plant fats or waxes in addition to cell binding proteins and optional additional components that assist in the growth of cultivated animal cells. The scaffold can exist in both a liquified state during sterilization and a solid state during the formation of the scaffold, the seeding of the cultivated cells, and the cellular growth phase. The scaffold is capable of remaining in the final product for consumption or is partially or completely melted out of the final product and recycled into raw material for forming new scaffolds.
This disclosure generally describes comestible cell-based food products including a combination of cultured cells and dry cell powder and methods for preparing such cell-based food products. In one or more embodiments, the comestible cell-based food product comprises a mixture of cultured animal cells and dry cultured animal cell powder. For example, the comestible cell-based food product is produced by generating a homogenous mixture of cultured animal cells and dry cultured animal cell powder. In one or more embodiments, the dry cultured animal cell powder improves the texture of the resultant comestible cell-based food product by tailoring the hardness and/or adhesiveness of the comestible cell-based food product.
A plant fat-based scaffold for growing cell-based meat products for consumption. The scaffold comprises primarily plant fats or waxes in addition to cell binding proteins and optional additional components that assist in the growth of cultivated animal cells. The scaffold can exist in both a liquified state during sterilization and a solid state during the formation of the scaffold, the seeding of the cultivated cells, and the cellular growth phase. The scaffold is capable of remaining in the final product for consumption or is partially or completely melted out of the final product and recycled into raw material for forming new scaffolds.
An assembly for delivering a fluid includes a fluid dispenser connected to a fluid supply conduit. The fluid dispenser includes a fluid outlet positioned along a length of the fluid dispenser. The fluid outlet is configured to deliver the fluid from the fluid supply conduit at a flow rate that varies along the length of the fluid dispenser.
B05B 3/10 - Appareillages de pulvérisation ou d'arrosage avec des éléments de sortie mobiles ou des éléments déflecteurs mobiles avec des éléments rotatifs avec évacuation sur la presque totalité de la périphérie de l'élément rotatif
B05B 3/04 - Appareillages de pulvérisation ou d'arrosage avec des éléments de sortie mobiles ou des éléments déflecteurs mobiles avec des éléments rotatifs entraînés par le liquide ou autre matériau fluide refoulé, p.ex. le liquide actionnant un moteur avant de parvenir à l'orifice de sortie
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
A plant fat-based scaffold for growing cell-based meat products for consumption. The scaffold comprises primarily plant fats or waxes in addition to cell binding proteins and optional additional components that assist in the growth of cultivated animal cells. The scaffold can exist in both a liquified state during sterilization and a solid state during the formation of the scaffold, the seeding of the cultivated cells, and the cellular growth phase. The scaffold is capable of remaining in the final product for consumption or is partially or completely melted out of the final product and recycled into raw material for forming new scaffolds.
An assembly for delivering a fluid includes a fluid dispenser connected to a fluid supply conduit. The fluid dispenser includes a fluid outlet positioned along a length of the fluid dispenser. The fluid outlet is configured to deliver the fluid from the fluid supply conduit at a flow rate that varies along the length of the fluid dispenser.
This disclosure generally describes comestible cell-based food products including a combination of cultured cells and dry cell powder and methods for preparing such cell-based food products. In one or more embodiments, the comestible cell-based food product comprises a mixture of cultured animal cells and dry cultured animal cell powder. For example, the comestible cell-based food product is produced by generating a homogenous mixture of cultured animal cells and dry cultured animal cell powder. In one or more embodiments, the dry cultured animal cell powder improves the texture of the resultant comestible cell-based food product by tailoring the hardness and/or adhesiveness of the comestible cell-based food product.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
66.
COMESTIBLE CELL-BASED MEAT PRODUCTS COMPRISING DRY CELL POWDER AND METHODS OF MAKING SUCH PRODUCTS
This disclosure generally describes comestible cell-based food products including a combination of cultured cells and dry cell powder and methods for preparing such cell-based food products. In one or more embodiments, the comestible cell-based food product comprises a mixture of cultured animal cells and dry cultured animal cell powder. For example, the comestible cell-based food product is produced by generating a homogenous mixture of cultured animal cells and dry cultured animal cell powder. In one or more embodiments, the dry cultured animal cell powder improves the texture of the resultant comestible cell-based food product by tailoring the hardness and/or adhesiveness of the comestible cell-based food product.
C12N 5/071 - Cellules ou tissus de vertébrés, p.ex. cellules humaines ou tissus humains
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
67.
Generation of Cell-Based Products for Human Consumption
The present disclosure relates to methods of preparing cell-based products for human consumption, in particular, from populations of such cell types as hepatocytes, adipocytes, myoblasts, and/or fibroblasts.
The apparatuses described herein relate to preparation of a meat product. Apparatuses, systems comprising the apparatuses, and methods of making and use the systems and apparatuses are described herein. These are useful for controlling one or more of growth on and separation of a meat product from an enclosed substrate. The apparatuses and systems are configured to receive fluid and grow the meat product and/or separate the meat product from the substrate in a scalable manner.
The present disclosure relates to methods of preparing cell-based products for human consumption, in particular, from populations of such cell types as hepatocytes, adipocytes, myoblasts, and/or fibroblasts.
The present disclosure relates to methods of preparing cell-based products for human consumption, in particular, from populations of such cell types as hepatocytes, adipocytes, myoblasts, and/or fibroblasts.
The present disclosure relates to methods of preparing cell-based products for consumption that comprise proteins from exotic, endangered, and extinct species, as well as cell-based products for consumption.
C12N 5/077 - Cellules mésenchymateuses, p.ex. cellules osseuses, cellules de cartilage, cellules stromales médulaires, cellules adipeuses ou cellules musculaires
A23L 13/00 - Produits à base de viande; Farine de viande; Leur préparation et leur traitement
A23J 1/02 - Préparation des compositions à base de protéines pour l'alimentation; Ouverture des œufs par grandes quantités et séparation du jaune du blanc à partir de la viande
A23J 3/34 - Traitement des protéines pour l'alimentation par hydrolyse utilisant des agents chimiques utilisant des enzymes
The present disclosure relates to methods of preparing cell-based products for consumption that comprise proteins from exotic, endangered, and extinct species, as well as cell-based products for consumption.
C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteurs; Vecteurs; Utilisation d'hôtes pour ceux-ci; Régulation de l'expression
C12N 5/077 - Cellules mésenchymateuses, p.ex. cellules osseuses, cellules de cartilage, cellules stromales médulaires, cellules adipeuses ou cellules musculaires
A23L 33/00 - Modification de la qualité nutritive des aliments; Produits diététiques; Leur préparation ou leur traitement
73.
METHODS FOR EXTENDING THE REPLICATIVE CAPACITY OF SOMATIC CELLS DURING AN EX VIVO CULTIVATION PROCESS
A product and process for extending the replicative capacity of metazoan somatic cells using targeted genetic amendments to abrogate inhibition of cell-cycle progression during replicative senescence and derive clonal cell lines for scalable applications and industrial production of metazoan cell biomass. An insertion or deletion mutation using guide RNAs targeting the first exon of the transcript encoding each protein is created using CRISPR/Cas9. Targeted amendments result in inactivation of p15 and p16 proteins which increases the proliferative capacity of the modified cell populations relative to their unaltered parental populations. Combining these amendments with ancillary telomerase activity from a genetic construct directing expression of a telomerase protein homolog from a TERT gene, increases the replicative capacity of the modified cell populations indefinitely. One application is to manufacture skeletal muscle for dietary consumption using cells from the poultry species Gallus gallus; another is from the livestock species Bos taurus.
C12N 15/11 - Fragments d'ADN ou d'ARN; Leurs formes modifiées
C12N 9/12 - Transférases (2.) transférant des groupes contenant du phosphore, p.ex. kinases (2.7)
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
A23L 33/135 - Bactéries ou leurs dérivés, p.ex. probiotiques
C12N 5/077 - Cellules mésenchymateuses, p.ex. cellules osseuses, cellules de cartilage, cellules stromales médulaires, cellules adipeuses ou cellules musculaires
The substrates, systems, and methods described herein relate to textured substrates for preparing a comestible meat product. Substrates and methods are described herein for controlling one or more of growth, adhesion, retention, and/or release of cells (e.g., of a cell sheet) on or from the surface of the substrate. A method of preparing a comestible meat product may include applying a plurality of non-human cells to at least one patterned texture substrate, growing the cells on the patterned texture substrate to form the comestible meat product, and separating the comestible meat product from the patterned texture substrate. The patterned texture allows for improved growth, adhesion, retention, and/or release of cells as compared to another surface not comprising the patterned texture. In some embodiments, the cell culture substrate surfaces include a plurality of regions corresponding to a plurality of patterned textures.
The substrates, systems, and methods described herein relate to textured substrates for preparing a comestible meat product. Substrates and methods are described herein for controlling one or more of growth, adhesion, retention, and/or release of cells (e.g., of a cell sheet) on or from the surface of the substrate. A method of preparing a comestible meat product may include applying a plurality of non-human cells to at least one patterned texture substrate, growing the cells on the patterned texture substrate to form the comestible meat product, and separating the comestible meat product from the patterned texture substrate. The patterned texture allows for improved growth, adhesion, retention, and/or release of cells as compared to another surface not comprising the patterned texture. In some embodiments, the cell culture substrate surfaces include a plurality of regions corresponding to a plurality of patterned textures.
Described herein are novel systems and methods for biomanufacturing, such as tissue cultivation. In some variations the systems and methods may comprise at least one porous substrate.
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
A23L 13/00 - Produits à base de viande; Farine de viande; Leur préparation et leur traitement
Disclosed herein are systems, devices, and methods for flowing fluid for radially expanded particle distribution within a laminar flow. In some variations, a system for cultivating tissue may comprise a bioreactor comprising an inlet, a substrate arranged in the bioreactor, and a diffusion module configured to transfer fluid from the inlet to the substrate. The diffusion module may comprise a porous material having at least one tortuous conduit extending between a first surface of the porous material and a second surface of the porous material.
Described herein are novel systems and methods for biomanufacturing, such as tissue cultivation. In some variations the systems and methods may comprise at least one porous substrate.
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p.ex. magnétisme, ondes sonores
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
Disclosed herein are systems, devices, and methods for flowing fluid for radially expanded particle distribution within a laminar flow. In some variations, a system for cultivating tissue may comprise a bioreactor comprising an inlet, a substrate arranged in the bioreactor, and a diffusion module configured to transfer fluid from the inlet to the substrate. The diffusion module may comprise a porous material having at least one tortuous conduit extending between a first surface of the porous material and a second surface of the porous material.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
B01D 69/02 - Membranes semi-perméables destinées aux procédés ou aux appareils de séparation, caractérisées par leur forme, leur structure ou leurs propriétés; Procédés spécialement adaptés à leur fabrication caractérisées par leurs propriétés
Provided herein are filter cake-based systems and methods for cultivating cells and cell biomass therefrom. Provided herein is a system for cultivating cells and cell biomass comprising a filter chamber comprising at least one inlet and at least one outlet, at least one filter support located within the filter chamber, and a filter cake located on the filter support, wherein the filter cake comprises at least one filter aid and a plurality of cells. Provided herein is a method for optimizing the cultivation of cells and cell biomass, comprising providing a filter support, adding at least one filter aid to the filter support, adding a plurality of cells to the filter aid, wherein the cells and the filter aid together comprise a filter cake, growing the cells into a cell biomass in the filter cake, wherein the filter cake is at least partially compressible.
The apparatuses described herein relate to preparation of a meat product. Apparatuses, systems comprising the apparatuses, and methods of making and use the systems and apparatuses are described herein. These are useful for controlling one or more of growth on and separation of a meat product from an enclosed substrate. The apparatuses and systems are configured to receive fluid and grow the meat product and/or separate the meat product from the substrate in a scalable manner.
The present disclosure provides methods and compositions useful for the production of slaughter-free meat products, and the characterizations of the same. The slaughter-free meat products contain several points of distinction when compared to conventional meat procured by harvesting the tissue of a dead animal. Such points of distinction include, but are not limited to, significantly reduced or substantially no: steroid hormones, antibiotics, or microbial contamination; lower fat content; no vasculature; and extended shelf life both at room temperature and when refrigerated.
C12N 5/071 - Cellules ou tissus de vertébrés, p.ex. cellules humaines ou tissus humains
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
C12N 5/077 - Cellules mésenchymateuses, p.ex. cellules osseuses, cellules de cartilage, cellules stromales médulaires, cellules adipeuses ou cellules musculaires
84.
SYSTEMS, DEVICES, AND METHODS FOR STERILIZING BIOREACTORS AND CULTURE MEDIA
Disclosed herein are systems, devices, and methods for sterilization. In some variations, a method of sterilizing a bioreactor system may comprise circulating a sterilant in a bioreactor and an enclosed vessel in fluidic communication with the bioreactor. The enclosed vessel may comprise cell culture media. The sterilant may be circulated for a dwell time sufficient to sterilize at least one of the bioreactor, the vessel, and the cell culture media.
Disclosed herein are systems, devices, and methods for sterilization. In some variations, a method of sterilizing a bioreactor system may comprise circulating a sterilant in a bioreactor and an enclosed vessel in fluidic communication with the bioreactor. The enclosed vessel may comprise cell culture media. The sterilant may be circulated for a dwell time sufficient to sterilize at least one of the bioreactor, the vessel, and the cell culture media.
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 3/04 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus comportant des moyens fournissant des couches minces
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p.ex. par des compteurs de colonies
86.
SYSTEMS, DEVICES, AND METHODS FOR STERILIZING BIOREACTORS AND CULTURE MEDIA
Disclosed herein are systems, devices, and methods for sterilization. In some variations, a method of sterilizing a bioreactor system may comprise circulating a sterilant in a bioreactor and an enclosed vessel in fluidic communication with the bioreactor. The enclosed vessel may comprise cell culture media. The sterilant may be circulated for a dwell time sufficient to sterilize at least one of the bioreactor, the vessel, and the cell culture media.
The present disclosure provides methods and compositions useful for the production of slaughter-free meat products, and the characterizations of the same. The slaughter-free meat products contain several points of distinction when compared to conventional meat procured by harvesting the tissue of a dead animal. Such points of distinction include, but are not limited to, significantly reduced or substantially no: steroid hormones, antibiotics, or microbial contamination; lower fat content; no vasculature; and extended shelf life both at room temperature and when refrigerated.
The apparatuses described herein relate to preparation of a meat product. Apparatuses, systems comprising the apparatuses, and methods of making and use the systems and apparatuses are described herein. These are useful for controlling one or more of growth on and separation of a meat product from an enclosed substrate. The apparatuses and systems are configured to receive fluid and grow the meat product and/or separate the meat product from the substrate in a scalable manner.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
A23L 13/00 - Produits à base de viande; Farine de viande; Leur préparation et leur traitement
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
The apparatuses described herein relate to preparation of a meat product. Apparatuses, systems comprising the apparatuses, and methods of making and use the systems and apparatuses are described herein. These are useful for controlling one or more of growth on and separation of a meat product from an enclosed substrate. The apparatuses and systems are configured to receive fluid and grow the meat product and/or separate the meat product from the substrate in a scalable manner.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
Provided herein are compositions and methods to make and use engineered cells, for the purpose of increasing the cell density of a culture comprising metazoan cells and for the production of a cultured edible product.
29 - Viande, produits laitiers et aliments préparés ou conservés
35 - Publicité; Affaires commerciales
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Meat; seafood; beef, pork, fish, poultry and game, not live; all natural meats and seafood; cultured meat and seafood; laboratory-grown meat and seafood; synthetic meat and seafood; tissue engineered meat and seafood; snack foods, prepared meals, salads and ready-to-eat meals consisting primarily of meat, fish, poultry, game, seafood and cultured meats; meat stocks; meat extracts. Retailing in the field of all natural meats and seafood, cultured meat and seafood, laboratory-grown meat and seafood, synthetic meat and seafood, and tissue engineered meat and seafood; wholesaling in the field of all natural meats and seafood, cultured meat and seafood, laboratory-grown meat and seafood, synthetic meat and seafood, and tissue engineered meat and seafood. Research and development in the field of prepared food and ingredient manufacturing from animal cells cultivated within bioreactors, as well as from non-animal sources including plants, fungi, algae and bacteria; scientific and laboratory research in the field of cultured meat and seafood, laboratory-grown meat and seafood, synthetic meat and seafood, and tissue engineered meat and seafood; research on food; research and development services in the field of cultured meat and seafood, laboratory-grown meat and seafood, synthetic meat and seafood, and tissue engineered meat and seafood.
29 - Viande, produits laitiers et aliments préparés ou conservés
35 - Publicité; Affaires commerciales
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
(1) Meat; seafood; beef, pork, fish, poultry and game, not live; all natural meats and seafood; cultured meat and seafood; laboratory-grown meat and seafood; synthetic meat and seafood; tissue engineered meat and seafood; snack foods, prepared meals, salads and ready-to-eat meals consisting primarily of meat, fish, poultry, game, seafood and cultured meats; meat stocks; meat extracts; (1) Retailing in the field of all natural meats and seafood, cultured meat and seafood, laboratory-grown meat and seafood, synthetic meat and seafood, and tissue engineered meat and seafood; wholesaling in the field of all natural meats and seafood, cultured meat and seafood, laboratory-grown meat and seafood, synthetic meat and seafood, and tissue engineered meat and seafood;
(2) Research and development in the field of prepared food and ingredient manufacturing from animal cells cultivated within bioreactors, as well as from non-animal sources including plants, fungi, algae and bacteria; scientific and laboratory research in the field of cultured meat and seafood, laboratory-grown meat and seafood, synthetic meat and seafood, and tissue engineered meat and seafood; research on food; research and development services in the field of cultured meat and seafood, laboratory-grown meat and seafood, synthetic meat and seafood, and tissue engineered meat and seafood
The present disclosure provides methods and compositions useful for the production of slaughter-free meat products, and the characterizations of the same. The slaughter-free meat products contain several points of distinction when compared to conventional meat procured by harvesting the tissue of a dead animal. Such points of distinction include, but are not limited to, significantly reduced or substantially no: steroid hormones, antibiotics, or microbial contamination; lower fat content; no vasculature; and extended shelf life both at room temperature and when refrigerated.
A23J 1/00 - Préparation des compositions à base de protéines pour l'alimentation; Ouverture des œufs par grandes quantités et séparation du jaune du blanc
C12N 5/071 - Cellules ou tissus de vertébrés, p.ex. cellules humaines ou tissus humains
C12N 5/077 - Cellules mésenchymateuses, p.ex. cellules osseuses, cellules de cartilage, cellules stromales médulaires, cellules adipeuses ou cellules musculaires
A23L 35/00 - Aliments ou produits alimentaires non prévus par les groupes ; Leur préparation ou leur traitement
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
The present disclosure provides methods and compositions useful for the production of slaughter-free meat products, and the characterizations of the same. The slaughter-free meat products contain several points of distinction when compared to conventional meat procured by harvesting the tissue of a dead animal. Such points of distinction include, but are not limited to, significantly reduced or substantially no: steroid hormones, antibiotics, or microbial contamination; lower fat content; no vasculature; and extended shelf life both at room temperature and when refrigerated.
C12N 5/071 - Cellules ou tissus de vertébrés, p.ex. cellules humaines ou tissus humains
C12N 5/077 - Cellules mésenchymateuses, p.ex. cellules osseuses, cellules de cartilage, cellules stromales médulaires, cellules adipeuses ou cellules musculaires
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p.ex. lignées cellulaires; Tissus; Leur culture ou conservation; Milieux de culture à cet effet
A23L 35/00 - Aliments ou produits alimentaires non prévus par les groupes ; Leur préparation ou leur traitement
A23J 1/00 - Préparation des compositions à base de protéines pour l'alimentation; Ouverture des œufs par grandes quantités et séparation du jaune du blanc
The present disclosure provides methods and compositions useful for the production of slaughter-free meat products, and the characterizations of the same. The slaughter-free meat products contain several points of distinction when compared to conventional meat procured by harvesting the tissue of a dead animal. Such points of distinction include, but are not limited to, significantly reduced or substantially no: steroid hormones, antibiotics, or microbial contamination; lower fat content; no vasculature; and extended shelf life both at room temperature and when refrigerated.
30 - Aliments de base, thé, café, pâtisseries et confiseries
Produits et services
Coffee beverages; tea beverages; sugar; pasta and noodles; bakery goods; cereal-based and rice-based snack food; meals consisting primarily of pasta and meat; ready-made dishes consisting primarily of pasta and meat; sandwiches; pies containing meat; treacle and sauces; seasonings, flavorings and condiments.
30 - Aliments de base, thé, café, pâtisseries et confiseries
32 - Bières; boissons non alcoolisées
33 - Boissons alcoolisées à l'exception des bières
43 - Services de restauration (alimentation); hébergement temporaire
Produits et services
Coffee beverages; tea beverages; sugar; pasta and noodles; bakery goods; cereal-based and rice-based snack food; meals consisting primarily of pasta and meat; ready-made dishes consisting primarily of pasta and meat; sandwiches; pies containing meat; treacle and sauces; seasonings, flavorings and condiments. Beer; non-alcoholic beverages; mineral water [beverages] and aerated water; fruit drinks; sports drinks; preparations for making beverages. Alcoholic beverages (except beer); pre-mixed alcoholic beverages, other than beer-based; spirits [beverages]; liqueurs; wine and wine-based beverages; fruit extracts, alcoholic. Services for providing food and drink, especially restaurant services; temporary accommodation, especially hotel services; rental of furniture, linens, table settings, and equipment for the provision of food and drink.
29 - Viande, produits laitiers et aliments préparés ou conservés
35 - Publicité; Affaires commerciales
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Meat; seafood; beef, pork, fish, poultry and game, not live; all natural meats and seafood; cultured meat and seafood; laboratory-grown meat and seafood; synthetic meat and seafood; tissue engineered meat and seafood; snack foods, prepared meals, salads and ready-to-eat meals consisting primarily of meat, fish, poultry, game, seafood and cultured meats; meat stocks; meat extracts. retailing in the field of all natural meats and seafood, cultured meat and seafood, laboratory-grown meat and seafood, synthetic meat and seafood, and tissue engineered meat and seafood; wholesaling in the field of all natural meats and seafood, cultured meat and seafood, laboratory-grown meat and seafood, synthetic meat and seafood, and tissue engineered meat and seafood. Research and development in the field of prepared food and ingredient manufacturing from animal cells cultivated within bioreactors, as well as from non-animal sources including plants, fungi, algae and bacteria; scientific and laboratory research in the field of cultured meat and seafood, laboratory-grown meat and seafood, synthetic meat and seafood, and tissue engineered meat and seafood; research on food; research and development services in the field of cultured meat and seafood, laboratory-grown meat and seafood, synthetic meat and seafood, and tissue engineered meat and seafood.
