Abrégé

The present invention relates to a method for controlling the particle size of a biocompatible hyaluronic acid filler, wherein in a method for preparing a filler having non-cohesive hardness through pressure alone, when the filler is extruded and transferred through a conveying pipe using the pressure of a syringe, filler particles are broken by hardness and thus the particle size is reduced, and the particle size of the filler can be controlled by repeatedly extruding and transferring the filler while changing the inner diameter of the conveying pipe in stages.

Classes IPC  ?

• A61L 27/20 - Polysaccharides
• A61F 2/00 - Filtres implantables dans les vaisseaux sanguinsProthèses, c.-à-d. éléments de substitution ou de remplacement pour des parties du corpsAppareils pour les assujettir au corpsDispositifs maintenant le passage ou évitant l'affaissement de structures corporelles tubulaires, p. ex. stents
• A61J 3/00 - Dispositifs ou procédés spécialement conçus pour donner à des produits pharmaceutiques une forme physique déterminée ou une forme propre à leur administration
• A61J 1/20 - Dispositions pour le transfert des liquides, p. ex. du flacon à la seringue
• A61M 5/178 - Seringues
• A61M 5/31 - Seringues Parties constitutives

Abrégé

A centrifugal separator of the present invention is designed to centrifuge a biological sample, collected by a syringe device, by rotating the syringe device which comprises: a syringe in which the collected biological sample is contained; and a first holder and a second holder for sealing the leading and trailing ends of the syringe, respectively. The centrifugal separator comprises: a rotator; a bracket which cooperates with the rotator and receives a portion of the leading end of the syringe while placing the syringe upright, so as to rotate the syringe about the longitudinal axis thereof; and a sample processing part which supports the rotation of the syringe and supplies a fluid into the syringe while sealing the inner space of the syringe, so as to wash out unnecessary components from the biological sample after the centrifugation and discharge the same outside the syringe.

Classes IPC  ?

• B04B 5/02 - Centrifugeurs constitués par plusieurs tambours indépendants tournant autour d'un axe situé entre les tambours
• B04B 15/06 - Autres accessoires pour centrifugeurs pour nettoyer les tambours, les filtres, les tamis, les garnitures ou similaires
• B04B 9/02 - Entraînement par moteur électrique

Abrégé

The present invention relates to a method of separating cultured cells, which is used in a configuration including: a first accommodation space; a cell suspension which is present in a liquid in the first accommodation space in a state in which a plurality of cells is connected to one another; a filter container which is hermetically connected to the first accommodation space and mounted with a cell dividing member having one or more through holes having an average diameter of 5 μm to 300 μm; and a second accommodation space which is hermetically connected to the filter container, in which the cell suspension flows from the first accommodation space to the second accommodation space through the filter, such that the connection between the cells is passively and mechanically separated while the cells collide with a fixed resistance portion of the filter.

Classes IPC  ?

• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
• C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
• C12M 1/26 - Inoculateur ou échantillonneur
• C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
• C12N 1/02 - Séparation des micro-organismes de leurs milieux de culture
• C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
• C12Q 1/24 - Méthodes d'échantillonnage, d'inoculation ou de développement d'un échantillonMéthodes pour isoler physiquement un micro-organisme intact
• G01N 1/28 - Préparation d'échantillons pour l'analyse
• G01N 1/40 - Concentration des échantillons
• G01N 33/18 - Eau

Abrégé

A freeze-drying method of the present invention enables a material to be obtained and a material to be excluded to be separated from a material through a procedure of, while bringing the material into contact with dry ice to allow the material to have a freezing temperature, exposing the material to a vacuum environment to remove moisture from the material by sublimation of the dry ice and the vacuum, and, at the same time, a dewatering procedure by rotation of the material.

Classes IPC  ?

• C12M 1/26 - Inoculateur ou échantillonneur
• C12M 1/10 - Appareillage pour l'enzymologie ou la microbiologie montés rotativement
• F26B 5/06 - Procédés de séchage d'un matériau solide ou d'objets n'impliquant pas l'utilisation de chaleur par évaporation ou sublimation de l'humidité sous une pression réduite, p. ex. sous vide le procédé impliquant la congélation
• C12N 5/077 - Cellules mésenchymateuses, p. ex. cellules osseuses, cellules de cartilage, cellules stromales médulaires, cellules adipeuses ou cellules musculaires

Abrégé

The present invention relates to an intercellular separation method for cultured cells, and provides an intercellular separation method for cultured cells, which is a method for separating cultured cells in a culture state, the method being characterized by using a first receiving space; a cell suspension in which a plurality of cells are present in a state of being connected to one another within liquid of the first receiving space; a filter vessel which is air-tightly connected with the first receiving space and is equipped with a cell-dividing member having one or more through-holes with an average diameter of 5 µm to 300 µm; and a second receiving space which is air-tightly connected with the filter vessel, wherein the cell suspension is moved from the first receiving space to the second receiving space via a filter so that the cell suspension allows intercellular linkages to be passively and mechanically separated while contacting a fixed resistance part of the filter.

Classes IPC  ?

• C12Q 1/24 - Méthodes d'échantillonnage, d'inoculation ou de développement d'un échantillonMéthodes pour isoler physiquement un micro-organisme intact
• C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse
• G01N 1/28 - Préparation d'échantillons pour l'analyse

Abrégé

Disclosed is a method of continuously culturing cells, including culturing the cells by injecting a culture medium into an internal compartment of a sealed culture vessel and then inoculating the culture medium with the cells, detaching the adhered cells when the density of the cells cultured in the compartment of the culture vessel is a reference value or more, and obtaining the cells detached from the culture vessel, which is maintained in a sealed state.

Classes IPC  ?

• C12M 1/26 - Inoculateur ou échantillonneur
• C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
• C12M 1/04 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz
• C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
• C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet

Abrégé

The present invention relates to a valve and a method for coupling the same. The valve is installed in a mechanism, which comprises a needle, a hub connected to the needle, and a connection part connected to the hub, so as to prevent fluid, filled in an apparatus for injecting the fluid into a living body, from being exposed to air. The connection part is coupled to an inner space of the hub, wherein the valve is installed in a dead space left in the inner space, except the portion to which the connection part is coupled. Thus, the present invention prevents fluid from flowing into the dead space by minimizing the volume of the dead space through the valve, and at the same time allows the fluid to flow through the valve.

Classes IPC  ?

• A61M 5/32 - AiguillesParties constitutives des aiguilles relatives au raccordement de celles-ci à la seringue ou au manchonAccessoires pour introduire l'aiguille dans le corps ou l'y maintenirDispositifs pour la protection des aiguilles
• A61M 5/40 - Dispositifs pour faire pénétrer des agents dans le corps par introduction sous-cutanée, intravasculaire ou intramusculaireAccessoires à cet effet, p. ex. dispositifs de remplissage ou de nettoyage, appuis-bras avec des moyens pour éliminer l'air ou pour empêcher l'injection ou l'introduction d'air dans le corps utilisant une soupape à flotteur pour couper l'écoulement des agents provenant du réservoir lorsque le niveau est trop bas
• A61M 39/22 - Soupapes ou agencement de soupapes

Abrégé

The method for continuous cell culture according to the present invention comprises the steps of: injecting a culture medium into the interior space of a sealed culture vessel and then inoculating cells to culture same; detaching attached cells if the density of the cells which have been cultured in the culture vessel reaches a reference value; and obtaining the detached cells in the culture vessel which is maintained sealed.

Classes IPC  ?

• C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
• C12M 3/02 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus comportant des moyens fournissant des suspensions
• C12M 3/06 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus avec des moyens de filtration, d'ultrafiltration, d'osmose inverse ou de dialyse
• C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores

Abrégé

The present invention provides an apparatus and a method for purifying a fine particle material, the apparatus being an apparatus for increasing the purity of a biochemical material in the form of a plurality of fine particles and comprising: an external container having a predetermined volume; a first member having a through hole through which a complex material including a biochemical material can pass, the first member being formed from a filter material having a plurality of pores of a size smaller than the biochemical material; and a transfer means using positive pressure at one end and/or negative pressure at the other end so that the complex material can pass through the first member, wherein, while the complex material is passing through the first member, gas, liquid, and particles smaller than the particles of the biochemical material can be discharged into the external container by means of gravity and/or pressure gradation.

Classes IPC  ?

• C12M 1/12 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens de stérilisation, filtration ou dialyse

Abrégé

The present invention relates to static pressure and a metering pump, the pump having a hollow interior and allowing a fluid to flow only in one direction, in which when pressure is applied to the pump, the internal volume decreases and a positive pressure is generated, and when the pressure is removed, the internal volume increases due to the elastic restoring power of the pump and a negative pressure is generated, thus allowing improved manipulability and enhanced accuracy.

Classes IPC  ?

• A61M 5/145 - Perfusion sous pression, p. ex. utilisant des pompes utilisant des réservoirs sous pression, p. ex. au moyen de pistons
• A61M 5/142 - Perfusion sous pression, p. ex. utilisant des pompes

Abrégé

A syringe for injecting a surgical thread, according to the present invention, relates a syringe comprising an insertion portion, an injection portion, and a front end portion that is formed on the injection portion, wherein the surgical thread having a plurality of protrusions is provided inside the injection portion, wherein the ends of the protrusions are formed in the opposite direction of the front end portion, and the surgical thread is not entirely accommodated in the injection portion but the end of the surgical thread is partially exposed through a space formed on the insertion portion, by pushing the surgical thread toward the front end portion by using a pushing means, which is coupled to the insertion portion, so that one side of the surgical thread is exposed to the exterior.

Classes IPC  ?

• A61M 5/158 - Aiguilles
• A61M 5/142 - Perfusion sous pression, p. ex. utilisant des pompes
• A61B 17/34 - TrocartsAiguilles à ponction
• A61M 37/00 - Autres appareils pour introduire des agents dans le corpsPercutanisation, c.-à-d. introduction de médicaments dans le corps par diffusion à travers la peau

Abrégé

The present invention relates to a tool for cutting a surgical thread, and the tool for cutting the surgical thread is provided therein with a cutter blade for cutting the end of the surgical thread in a lengthwise direction so as to split the surgical thread, and for guiding repeated insertions of the surgical thread so as to be split into multiple threads, which cuts the surgical thread so that the end of the surgical thread is split in the lengthwise direction, wherein the tool for cutting guides the entry so that the end of the surgical thread aligns in the cutter blade so that the end of the surgical thread can be split into multiple threads when the surgical thread is repeatedly entered, thereby allowing the surgical thread to stay in place after a surgery due to the end of the surgical thread that is split into multiple threads by means of the tool for cutting, which effectively enhances accuracy in wrinkle removal.

Classes IPC  ?

• A61B 19/00 - Instruments, outillage ou accessoires pour la chirurgie ou le diagnostic non couverts par l'un des groupes A61B 1/00-A61B 18/00, p.ex. pour stéréotaxie, opération aseptique, traitement de la luxation, protecteurs des bords des blessures(masques de protection du visage A41D 13/11; blouses de chirurgien ou vêtements pour malades A41D 13/12; dispositifs pour retirer, traiter ou transporter les liquides du corps A61M 1/00)
• A61B 17/32 - Instruments chirurgicaux coupants

Abrégé

A centrifuge includes a rotator comprising a receiving unit receiving materials and guiding the materials to move upward by centrifugal force to an upper portion of the receiving unit, and a rotator cover covering the upper portion of the receiving unit. The centrifuge also includes a container that is coupled to the rotator cover to be connected to the receiving unit and that receives the materials moved upward along the receiving unit. The centrifuge and a centrifuging method as disclosed herein are used for separating a fluid to which a large centrifugal force is applied and a fluid to which a smaller centrifugal force is applied by adjusting the rotation speed using the principle that centrifugal force during rotation varies according to specific components of a fluid. Thus, using the centrifuge and the centrifugal method, materials can be centrifuged and layers of the materials can be classified and collected accurately and easily.

Classes IPC  ?

• B04B 5/02 - Centrifugeurs constitués par plusieurs tambours indépendants tournant autour d'un axe situé entre les tambours

Abrégé

The present invention relates to a novel hepatitis A virus antigen gene and transformed plants with the gene, more particularly to a novel hepatitis A virus antigen gene synthesized by optimization of a genetic code, a recombinant vector containing the gene and plants transformed with the recombinant vector. According to the invention, the use of a synthesized antigen gene of hepatitis A virus makes it possible to prepare recombinant hepatitis A virus vaccine materials at a high efficiency, and transformed plants expressing a hepatitis A virus antigen are useful as functional food materials and orally administrated vaccine materials.

Classes IPC  ?

• C12N 15/33 - Gènes codant pour des protéines virales
• C12N 15/63 - Introduction de matériel génétique étranger utilisant des vecteursVecteurs Utilisation d'hôtes pour ceux-ciRégulation de l'expression

Abrégé

The present invention relates to a method for stably preparing the VPl or VP2 antigen protein of hepatitis A virus using insect cells transformed with a recombinant vector containing the antigen gene of hepatitis A virus. When the transformed insect cells expressing the antigen gene of hepatitis A virus is used, a recombinant hepatitis A virus antigen can be stably prepared, and it can be used as a vaccine.

Classes IPC  ?

• C12N 15/85 - Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes pour cellules animales

Abrégé

Provided are a centrifuge and a centrifugal method in which layers of a centrifuged material can be classified and collected accurately and easily without a separation or washing process by opening or closing an opening portion of a container in a centrifuging process. The centrifuge includes: a rotator; a container into which a material is injected and comprising an opening portion, inclined at a predetermined angle with respect to a rotation axis of the rotator such that the opening portion is directed downward, and coupled to the rotator; and a stopper portion that closes the opening portion, and when the speed of the rotator reaches a predetermined speed, thereby centrifuging the material, opens the opening portion such that the material in the container flows outward.

Classes IPC  ?

• B04B 1/06 - Centrifugeurs à tambours rotatifs à parois pleines pour la séparation de mélanges essentiellement liquides contenant ou non des particules solides avec cloisons intérieures de forme cylindrique

Abrégé

A centrifuge includes a rotating body capable of rotating and having an accommodation portion guiding a substance contained in the accommodation portion to move toward a top side due to a centrifugal force according to a rotation of the rotating body, a filter installed at the top side of the accommodation portion and allowing a part of the substance moving to the top side of the accommodation portion to pass through the filter, and a rotating body cover installed above the filter, covering the top side of the accommodation portion, and guiding the substance passing through the filter to be exhausted to the outside.

Classes IPC  ?

• B04B 1/06 - Centrifugeurs à tambours rotatifs à parois pleines pour la séparation de mélanges essentiellement liquides contenant ou non des particules solides avec cloisons intérieures de forme cylindrique

Abrégé

Provided is a centrifuge that can accurately and easily separate and collect layers of centrifuged material, the centrifuge including: a rotator comprising a receiving unit receiving materials inside and guiding the material to move upward by centrifugal force, and a rotator cover covering an upper portion of the receiving unit; and a container that is coupled to the rotator cover to be connected to the receiving unit and collects the material moved to the upper portion of the receiving unit. Also provided is a centrifuging method in which after centrifuging layers of the material by the centrifuge, the rotation speed of the centrifuge is reduced to separate a fluid to which a large centrifugal force is applied and a fluid to which a smaller centrifugal force is applied by adjusting the rotation speed, using the principle that centrifugal force during rotation varies according to specific gravity of components of a fluid, thereby accurately classifying and collecting layers of a desired material.

Classes IPC  ?

• B04B 1/08 - Centrifugeurs à tambours rotatifs à parois pleines pour la séparation de mélanges essentiellement liquides contenant ou non des particules solides avec cloisons intérieures de forme conique