01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Biochemical reagents for use in scientific, environmental,
food, and forensic research; reagent kits comprised of
biochemical reagents for use in scientific, environmental,
food and forensic research; biochemical reagents for use in
a scientific apparatus for chemical or biological analysis;
reagent kits comprised of biochemical reagents for use in a
scientific apparatus for chemical or biological analysis.
Disclosed herein are aspects of a composition, typically comprising a functionalized magnetic particle with an outer surface comprising a ligand on the outer surface, wherein the ligand is bound to a permeabilized cell comprising a nucleic acid for generating a nucleic acid library. Also disclosed are a method of tagging a nucleic acid within at least one cell, a method of generating a nucleic acid library, systems configured for processing disclosed compositions and implementing disclosed methods, and kits comprising a composition or compositions for use with disclosed method aspects.
C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
Systems and method for determining variants can receive mapped reads and determine a distribution of matched-filter residuals distribution from a plurality of reads at a homopolymer region. The distribution of matched-filter residuals can be fit to uni-modal and bi-modal models. Based on the model that best fits the distribution of matched-filter residuals, the heterozygosity of the sample and the absence or presence of an insertion/deletion in the homopolymer can be determined.
Cell analysis systems and instruments provide an end user with time-course graphic and imaging data of individual cellular responses as elicited by perturbations in the cellular microenvironment and monitored by ChemFET sensors. Imaging data of cellular responses can be presented as an electroscopic image, which is an image taken at a defined sampling interval of cellular response from sensors covering a cell footprint or an image of cellular response for sensors detecting cellular effluent. Electroscopic imaging can be compared to optical imaging to provide an additional dimension of information regarding cell analysis. For example, comparing optical imaging of immunocytochemical studies using known marker panels provides a functional and phenotypic signature of data obtained for each cell analyzed using a ChemFET-based cell analysis system.
An artificial neural network is applied to a plurality of flow predictor features to generate a flow space probability of error for a base call. A base quality value for the base call is determined based on the flow space probability of error. The base call and flow predictor features are based on the flow space signal measurements generated in response to the nucleotide flow to the reaction confinement region. For an array of reaction confinement regions, a plurality of parallel neural networks is applied to produce a probability of error for each reaction confinement region. A given neural network of the parallel neural networks is applied to the plurality of flow predictor features corresponding to a given reaction confinement region in the array to provide the flow space probability of error for the given reaction confinement region.
A method for nucleic acid sequencing includes: receiving a signal comprising measurements of a parameter measured in response to a plurality of nucleotide flows flowed in a space comprising a sample nucleic acid; normalizing the signal to obtain a normalized signal; adaptively normalizing the normalized signal to obtain an adaptively normalized signal; and predicting a sequence of base calls corresponding to the sample nucleic acid using the adaptively normalized signal.
G16B 30/00 - ICT specially adapted for sequence analysis involving nucleotides or amino acids
G01N 27/27 - Association of two or more measuring systems or cells, each measuring a different parameter, where the measurement results may be either used independently, the systems or cells being physically associated, or combined to produce a value for a further parameter
G01N 27/414 - Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
G16B 25/00 - ICT specially adapted for hybridisationICT specially adapted for gene or protein expression
Sample preparation methods for in situ RNA or DNA analysis, methods and compositions used in such methods are provided. Methods provided herein allow DNA or RNA preparation and downstream analysis to be carried out in the same tube or on an aliquot of the prepared sample without centrifugation or further purification. The compositions and methods provided herein can advantageously be used on a variety of samples, including cultures of cell lines and/or primary cells. The preparation process is amenable to high throughput processing using manual or robotic platforms.
A method for user guided initiating of an instrument includes receiving a run plan via a user interface of the instrument; indicating on the user interface, based on the run plan, a consumable to be provided to the instrument; detecting the presence of the consumable using a vision system; and indicating the presence of the consumable via the user interface.
Ionizable lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
C07C 215/28 - Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being unsaturated and containing six-membered aromatic rings
C07C 217/42 - Compounds containing amino and etherified hydroxy groups bound to the same carbon skeleton having etherified hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having etherified hydroxy groups and at least two amino groups bound to the carbon skeleton
C07C 219/06 - Compounds containing amino and esterified hydroxy groups bound to the same carbon skeleton having esterified hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having the hydroxy groups esterified by carboxylic acids having the esterifying carboxyl groups bound to hydrogen atoms or to acyclic carbon atoms of an acyclic saturated carbon skeleton
C07C 219/08 - Compounds containing amino and esterified hydroxy groups bound to the same carbon skeleton having esterified hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having at least one of the hydroxy groups esterified by a carboxylic acid having the esterifying carboxyl group bound to an acyclic carbon atom of an acyclic unsaturated carbon skeleton
C07C 229/12 - Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one amino and one carboxyl group bound to the carbon skeleton the nitrogen atom of the amino group being further bound to acyclic carbon atoms or to carbon atoms of rings other than six-membered aromatic rings to carbon atoms of acyclic carbon skeletons
C07C 229/22 - Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated the carbon skeleton being further substituted by oxygen atoms
C07C 229/26 - Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having more than one amino group bound to the carbon skeleton, e.g. lysine
C07C 229/36 - Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton containing six-membered aromatic rings with at least one amino group and one carboxyl group bound to the same carbon atom of the carbon skeleton
C07C 323/25 - Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and nitrogen atoms, not being part of nitro or nitroso groups, bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being acyclic and saturated
C07D 209/20 - Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals substituted additionally by nitrogen atoms, e.g. tryptophane
C07D 233/61 - Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members with only hydrogen atoms or radicals containing only hydrogen and carbon atoms, attached to ring carbon atoms with hydrocarbon radicals, substituted by nitrogen atoms not forming part of a nitro radical, attached to ring nitrogen atoms
C07C 215/14 - Compounds containing amino and hydroxy groups bound to the same carbon skeleton having hydroxy groups and amino groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being saturated and acyclic the nitrogen atom of the amino group being further bound to hydrocarbon groups substituted by amino groups
A61K 9/127 - Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
C07C 279/14 - Derivatives of guanidine, i.e. compounds containing the group the singly-bound nitrogen atoms not being part of nitro or nitroso groups having nitrogen atoms of guanidine groups bound to acyclic carbon atoms of a carbon skeleton being further substituted by carboxyl groups
Ionizable lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
The present invention provides mutant DNA polymerases, polynucleotides encoding the polymerases, cassettes and vectors including such polynucleotides, and cells containing the polymerases, polynucleotides, cassettes, and/or vectors of the invention. The present invention also provides methods for synthesizing polynucleotides and kits including a DNA polymerase of the invention.
A detection system that operates with reduced sample waste and dead volume, the system including: a module configured to introduce a sample spacer into a sample; at least one light source, wherein the light source illuminates the sample spacer and the sample, wherein illumination of the sample spacer produces scattered light; and a detection device configured to initiate acquisition of data related to the sample in response to scattered light detected by the detection device.
Examples described herein provide systems and methods for quantifying cells. An example method includes receiving at least one image, improving a contrast of the at least one image to generate a contrast image, and performing a fit operation on the contrast image to generate a processed image. The method includes applying a filter to the processed image to generate a filtered image, identifying cells within the filtered image, and providing an output image including an indication of the cells.
A detection system that operates with reduced sample waste and dead volume, the system including: a module configured to introduce a sample spacer into a sample; at least one light source, wherein the light source illuminates the sample spacer and the sample, wherein illumination of the sample spacer produces scattered light; and a detection device configured to initiate acquisition of data related to the sample in response to scattered light detected by the detection device.
A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.
Examples described herein provide systems and methods for quantifying cells. An example method includes receiving at least one image, improving a contrast of the at least one image to generate a contrast image, and performing a fit operation on the contrast image to generate a processed image. The method includes applying a filter to the processed image to generate a filtered image, identifying cells within the filtered image, and providing an output image including an indication of the cells.
G06V 10/50 - Extraction of image or video features by performing operations within image blocksExtraction of image or video features by using histograms, e.g. histogram of oriented gradients [HoG]Extraction of image or video features by summing image-intensity valuesProjection analysis
G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts
32.
SYSTEMS AND METHODS FOR DETECTING STRUCTURAL VARIANTS
Systems and method for identifying gene fusions can obtain sequencing information for a plurality of amplicons from a nucleic acid sample. The sequencing information can include a plurality of reads that are initially partially mapped to a reference sequence. Fragments may be generated by splitting the partially mapped reads into mapped and unmapped fragments, and the fragments may be remapped to the reference sequence. Gene fusions can be identified based on reads where the first fragment maps to a first gene and the second fragment maps to a second gene.
Campylobacter spSalmonella spShigellasp./Enteroinvasive Escherichia coli (EIEC)Escherichia coli Escherichia coliEscherichia coli stx2/Shiga toxin B. Other embodiments include methods and kits for detecting diarrhea causing pathogens.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
A61K 39/40 - AntibodiesImmunoglobulinsImmune serum, e.g. antilymphocytic serum bacterial
34.
METHODS FOR GAS FILTRATION IN FLUID PROCESSING SYSTEMS
A method for filtering a gas comprises passing a gas through a compartment of a filter assembly, the filter assembly comprising: an inlet opening; a first outlet opening; a casing comprising polymeric film and bounding the compartment, the compartment communicating with the inlet opening and the first outlet opening; and a first filter at least partially disposed within the compartment. The method further comprising forming a first seal across a first section of the casing at a location between the inlet opening and the first filter to form a first sub-compartment within the casing and severing the casing at a first location.
B01D 46/58 - Filters or filtering processes specially modified for separating dispersed particles from gases or vapours with multiple filtering elements, characterised by their mutual disposition connected in parallel
B01D 46/00 - Filters or filtering processes specially modified for separating dispersed particles from gases or vapours
B01F 23/231 - Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids by bubbling
B01F 27/2121 - Mixers with rotary stirring devices in fixed receptaclesKneaders characterised by their rotating shafts composed of interconnected parts
B01F 27/88 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with a separate receptacle-stirrer unit that is adapted to be coupled to a drive mechanism
B01F 27/90 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with paddles or arms
An instrument for processing and/or measuring a biological process comprises a sample processing system and an excitation source exhibiting a spectral function of output power or intensity verses wavelength of output power or intensity. The spectral function has a minima wavelength corresponding to a local minima value of the output power or intensity; a first maxima wavelength corresponding to a first local maxima of output power or intensity, the output power or intensity at the first local maxima being greater than the output power or intensity at any wavelength less than the minima wavelength; a second maxima wavelength corresponding to a second local maxima of output power or intensity, the output power or intensity at the second local maxima being greater than the output at any wavelength greater than the minima wavelength; the minima wavelength is between the first maxima wavelength and the second maxima wavelength.
G01N 21/27 - ColourSpectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection
36.
PURIFICATION CHEMISTRIES AND FORMATS FOR SANGER DNA SEQUENCING REACTIONS ON A MICRO-FLUIDICS DEVICE
According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.
The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C12Q 1/6881 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
38.
COMPOSITIONS AND METHODS FOR REDUCING MASTER MIX CONTAMINATION
This disclosure describes master mix compositions, nucleic acid amplification kits, methods of manufacturing master mix compositions, and methods of using master mix compositions that minimize or eliminate the negative effects of contaminant nucleic acids. Conventional master mix compositions often include contaminant nucleic acids.
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
The charging stand kit for assembling a charging stand (1) for electrical pipettes (10) comprises a charging module (2) configured to hold an electrical pipette (10) to be charged, the charging module (2) comprising first and second sets of electrical contacts (7, 8) on first and second sides for connecting the charging module (2) electrically to adjacent parts (2, 3) of the charging stand (1), a first leg (3) having an upper end con- figured to be attached to the first side of the charging module (2) and comprising a set of electrical contacts (9) configured to contact the first set of electrical contacts (7) of the charging module (2), and a second leg (4) having an upper end config- ured to be attached to the second side of the charging module (2).
A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
A method for sequencing a nucleic acid template includes: (a) performing a first sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a first predetermined ordering of nucleotides and/or reagents to obtain a first sequencing result; (b) after the first sequencing process, performing a second sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a second predetermined ordering of nucleotides and/or reagents to obtain a second sequencing result, the second predetermined ordering of nucleotides and/or reagents being different from the first predetermined ordering of nucleotides and/or reagents and at least one of the first and second predetermined orderings of nucleotides and/or reagents being designed for repeat sequencing; and (c) determining a sequence of bases corresponding to at least a portion of the nucleic acid template using both the first sequencing result and the second sequencing result.
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Chemicals for use in industry and science; diagnostic preparations for scientific or research use; diagnostic preparations for clinical or medical laboratory use; DNA polymerase, reagents and reagent kits comprising generic DNA circle, DNA polymerase and buffers for scientific, medical or veterinary research use; DNA polymerase, reagents and reagent kits comprising generic DNA circle, DNA primers, DNA polymerase and buffers for use in the biotechnology field
46.
LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR
Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.
C40B 20/04 - Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
C40B 40/06 - Libraries containing nucleotides or polynucleotides, or derivatives thereof
C40B 50/06 - Biochemical methods, e.g. using enzymes or whole viable microorganisms
47.
FLUID MIXING SYSTEMS WITH MODULAR IMPELLERS AND RELATED METHODS
A mixing system for mixing a liquid includes a first impeller segment having a first mount and a first mixing blade secured to the first mount and a second impeller segment having a second mount and a first mixing blade secured to the second mount, the second impeller segment being separate and discrete from the first impeller segment. One or more drive members are secured to the first impeller segment and the second impeller segment for concurrently rotating the first impeller segment and the second impeller segment about a rotational axis. The first impeller segment and the second impeller segment are secured to the one or more drive members so that a plane extending normal to the axis of rotation intersects with the first mixing blade of the first impeller segment and the first mixing blade of the second impeller segment.
B01F 27/191 - Stirrers with two or more mixing elements mounted in sequence on the same axis with similar elements
B01F 27/07 - Stirrers characterised by their mounting on the shaft
B01F 27/072 - Stirrers characterised by their mounting on the shaft characterised by the disposition of the stirrers with respect to the rotating axis
B01F 27/113 - Propeller-shaped stirrers for producing an axial flow, e.g. shaped like a ship or aircraft propeller
B01F 27/91 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with propellers
B01F 35/513 - Flexible receptacles, e.g. bags supported by rigid containers
B01F 101/44 - Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
C12M 1/06 - Apparatus for enzymology or microbiology with gas introduction means with agitator, e.g. impeller
48.
SYSTEMS AND METHODS FOR IDENTIFYING SEQUENCE VARIATION
Systems and method for determining variants can receive mapped reads, align flow space information to a flow space representation of a corresponding portion of the reference. Reads spanning a position with a potential variant can be evaluated in a context specific manner. A list of probable variants can be provided.
C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
G16B 5/00 - ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
A method for reducing spectral crosstalk in a multiplexed assay is provided. The method includes receiving filter signal data from a multiplexed fluorescence assay and an initial dye matrix including calibrated spectral dye data. The method further includes generating an updated dye matrix based on the initial dye matrix and estimated crosstalk proxies, and then adjusting the updated dye matrix to meet a calculated optimization value. The calculated optimization value is calculated based on at least the estimated crosstalk proxies and the filter signal data. The calculated optimization value may be further calculated based on each adjustment of the updated dye matrix, and a cross-correlation between dyes used in the assay. The method further includes generating an improved dye matrix based on the adjusted updated dye matrix, and then generating spectral adjusted data based on the improved dye matrix.
A system for sample holder scanning is provided. The system includes a light source, a multiband excitation filter configured to select at least two excitation bands of light for fluorescent dyes used in the sample holder. The system further includes a multiband dichroic filter configured to reflect the at least two excitation bands of light, a multiband emission filter configured to transmit at least two bands of fluorescent emission light from each reaction site of the sample holder. The multiband dichroic filter is further configured to transmit the at least two bands of fluorescent emission light. The system also includes an optical sensor configured to detect the at least two bands of fluorescent emission light to generate an image of a sample holder.
Assemblies, methods, and systems for delivering a fluid into a fluidic flow. An assembly includes: a dispenser body having an interior wall defining a lumen having a longitudinal axis and a diameter; a cap configured to traverse the diameter of the lumen, the cap having an upper surface and a lower surface; a piston disposed within the lumen, the piston having a top surface and a bottom surface, the top surface together with the interior wall and the lower surface of the cap defining a working volume of the lumen, wherein the piston is movable along the longitudinal axis to change the working volume; an outlet in fluid connection with the working volume; and a fluid conduit extending between a first portion of the fluid conduit and a second portion of the fluid conduit, the fluid conduit being in fluid connection with the working volume via the outlet between the first and second portions of the fluid conduit.
A61M 39/00 - Tubes, tube connectors, tube couplings, valves, access sites or the like, specially adapted for medical use
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
C12M 1/00 - Apparatus for enzymology or microbiology
A61J 1/05 - Containers specially adapted for medical or pharmaceutical purposes for collecting, storing or administering blood, plasma or medical fluids
A61M 5/168 - Means for controlling media flow to the body or for metering media to the body, e.g. drip meters, counters
A61M 5/145 - Pressure infusion, e.g. using pumps using pressurised reservoirs, e.g. by means of pistons
A61M 39/20 - Closure caps or plugs for connectors or open ends of tubes
52.
SYSTEMS AND METHODS OF CELL SORTING IMPLEMENTING ARTIFICIAL INTELLIGENCE
Disclosed herein are apparatuses, systems, as well as related methods, computing devices, and computer-readable media related to real time cell sorter cell sorting using embeddings. For example, in some embodiments a method may comprise receiving first cell sorter data. The first cell sorter data may include cell sorter data including microscopy data, hyperspectral imaging data, high-dimensional vector data, or one or more combinations thereof. In some embodiments, the cell sorter data may include quantitative fluorescence data expressed as one or more of antibodies bound per cell, antibody binding capacity (ABC), molecules of equivalent soluble fluorochrome (MESF), one or more other quantitative indicators of fluorescence, or one or more combinations thereof. In some embodiments, the quantitative fluorescence data includes one or more fluorescence signals from: one or more fluorescent proteins, one or more fluorescent dyes, one or more fluorescently conjugate antibodies, or one or more combinations thereof.
Disclosed herein are dual-expression polynucleotide vectors including a first polynucleotide sequence including, in the 5' to 3' direction, a bacteriophage promoter sequence operatively linked to a sequence encoding a plurality of first RNA hairpin structures, a multiple cloning site sequence, a sequence encoding a plurality of second RNA hairpin structures, and a transcription terminator sequence; and a second polynucleotide sequence including, in the 5' to 3' direction, a bacteriophage promoter sequence operatively linked to a ribosome binding site sequence, a viral coat protein sequence, and the transcription terminator sequence. RNA detection and/or quantification standards or controls produced from these dual-expression vectors, along with methods for producing the RNA detection and/or quantification standards or controls, and methods of detecting the presence or quantity of nucleic acid using the RNA detection and/or quantification standards, are also disclosed.
The present disclosure is directed to compositions, methods and kits useful for the synthesis of nucleic acid molecules. More specifically, compositions, methods and kits are provided for the amplification of nucleic acid molecules in an one-step qPCR or RT-qPCR procedure comprising a reverse transcriptase, a DNA polymerase, at least one dNTP, and at least one stabilizing agent, wherein said at least one stabilizing agent increases stability of an assembled polymerase chain reaction.
C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Disclosed are compositions, kits, and methods for amplifying and quantifying a target nucleic acid from a sample. Compositions, kits, and methods enable the quantification of a target nucleic acid from a sample using an internal quantification standard disposed in the same reaction volume as the target nucleic acid, thereby eliminating the need for additional amplification reactions and processing to generate a standard curve. Compositions, kits, and methods also enable the comparison of target nucleic acid loads between two or more test samples by normalizing measured levels of the target nucleic acid in each sample according to relative levels of endogenous nucleic acid in each test sample.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
56.
METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS
Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.
G01N 27/414 - Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
C12Q 1/6818 - Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
H01L 27/088 - Devices consisting of a plurality of semiconductor or other solid-state components formed in or on a common substrate including integrated passive circuit elements with at least one potential-jump barrier or surface barrier the substrate being a semiconductor body including only semiconductor components of a single kind including field-effect components only the components being field-effect transistors with insulated gate
H01L 29/423 - Electrodes characterised by their shape, relative sizes or dispositions not carrying the current to be rectified, amplified or switched
H01L 29/78 - Field-effect transistors with field effect produced by an insulated gate
57.
METHODS FOR CONTEXT BASED COMPRESSION OF GENOMIC DATA FOR IMMUNO-ONCOLOGY BIOMARKERS
The method includes compressing numbers of reads data for targeted genes of a gene expression assay performed on a test sample. The targeted genes are organized into categories. Each category represents a functional context associated with the targeted genes in that category. The numbers of reads corresponding to targeted genes each category is compressed to form a compressed value for the category. The compressed value is compared to a baseline value for the category to determine an enrichment or a loss of a signature corresponding to the functional context of the category. The method may include analyzing information from multiple assays performed on the test sample, assigning a score value to each assay result and predicting a response to immune-oncology treatment based on the assigned scores.
Methods, systems, and devices for analyzing a sample for the presence of a target protein are provided. A sample can be processed, the processing generating an amplification product of a template nucleic acid formed in response to a target protein being present in the sample. The amplification product can be contacted with a detection probe to generate a second product comprising a labeled nucleic acid. A lateral flow substrate can be contacted with the second product, the lateral flow substrate comprising a capture moiety configured to bind the labeled nucleic acid. The methods can be carried out in one or more chambers of a single device or multiple devices. Detection probe bound to amplicons of the template nucleic acid captured on the lateral flow substrate can be read visually or using a sensor.
Life Technologies Holdings PTE Limited (Singapore)
Life Technologies Corporation (USA)
Inventor
Liaw, Wui Khen Kevin
Boo, Kuan Moon
Yeo, Huei Steven
Foo, Wern Yuh
Mead, Joshua Mathew
Bong, Justina Linkai
Makinen, Mikko
Ling, Mio Xiu Lu
Lee, Way Xuang
Chan, Wen Lun
Liu, Yunxiang
Abstract
Multichannel pipettes, electroporation systems utilizing the multichannel pipettes and methods for electroporating a cell. The electroporation system includes a multichannel pipette, a pipette tip(s), a pipette docking assembly, and a pulse generator. The pipette docking assembly includes a pipette station, a pipette station guard, and a reservoir.
A rocking assembly for mixing and cooling a liquid, such as a cell containing liquid (e.g., cell-based therapeutic product), comprising a base unit (110) having a mount (120), a platform (130), a cooler module (150) operably connected to the platform and the mount, the cooler module configured to cool the platform; and an actuator in operable connection with the cooler module, the actuator being configured to move the platform in a rocking motion relative to the base unit. A cell processing system for fill and finish processes in the manufacture of cell-based therapeutics including the rocking assembly and a liquid dispensing system. A method for processing a liquid for fill and finish workflow applications.
An optical fiber apparatus and method for uniformly illuminating biological samples distributed across one or more arrays of microchambers of a microplate can establish more reliable Polymerase Chain Reaction (PCR) data sampling. In some embodiments, the apparatus comprises a light engine that emits a beam of light, which is captured by a fiber bundle for delivering one or more beams of light to each microchamber; whereby, each beam of light causes the biological sample to fluoresce. The fiber cables of the fiber bundle include distal output ends that are held in place using a mechanical stand, which serves to flexibly align the output beams of the distal output ends to be arranged such that the centers of the output light beams are directed to different locations in the one or more arrays of microchambers, where each of the adjacent ones of the output light beams partially overlap for uniform distribution of light.
Multichannel pipettes, electroporation systems utilizing the multichannel pipettes and methods for electroporating a cell. The electroporation system includes a multichannel pipette, a pipette tip(s), a pipette docking assembly, and a pulse generator. The pipette docking assembly includes a pipette station, a pipette station guard, and a reservoir.
The present disclosure provides systems and methods of electroporation protocol optimization. Embodiments include electroporation machines capable of carrying out test protocols including multiple user-designated parameters. The protocols and parameters can be carried out on samples comprising cells, including portions of a sample, to determine optimum parameters for electroporation for different samples. The systems and methods of optimization preferably use electroporation cartridges, electroporation instruments and systems and methods of electroporation using these devices and systems. In some embodiments, electroporation cartridges comprise an electroporation chamber and electrodes.
A carrier for holding a biological sample includes a substrate. The substrate is configured to engage a first sample chamber comprising a first opening characterized by a first opening diameter or a second sample chamber comprising a second opening characterized by a second opening diameter that is greater than the first opening diameter. The substrate includes an upper portion, a lower portion, and an intermediate portion disposed between the upper portion and the lower portion. The lower portion is disposed below the upper portion and comprises a bottom surface configured to receive a biological sample. The intermediate portion is characterized by a first substrate diameter and the lower portion is characterized by a second substrate diameter that is less than the first substrate diameter.
Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of SARS-CoV-2, including SARS-CoV-2 variants, or other coronaviruses from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
69.
Adjustable Foam Sensor Systems And Related Methods
An adjustable foam sensor system is disclosed. The adjustable foam sensor system can include an adjustable foam sensor coupled to a transition member with a foam probe extending from the transition member. The adjustable foam sensor system can also include an adjustable housing that can be coupled to a container interface for interfacing with a container of a fluid processing system. The adjustable housing can be configured to be compressed or extended in response to a force, which in turn can reposition or move the adjustable foam sensor in the container. A controller can automatically adjust the adjustable foam sensor based on sensed and/or determined fluid levels, as well as control the delivery of anti-foam for reducing foam in the container.
Provided herein are compositions, methods and uses that relate to or result from providing separation media having at least one flocculant ligand covalently attached to a base surface or support, and the separation and/or purification of biological molecules using the separation media of the present disclosure. Certain embodiments provide separation media which under certain modes of operation, enhance the separation of the molecule of interest from impurities.
B01J 39/05 - Processes using organic exchangers in the strongly acidic form
B01D 15/36 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
B01J 39/20 - Macromolecular compounds obtained by reactions only involving unsaturated carbon-to-carbon bonds
B01J 47/014 - Ion-exchange processes in generalApparatus therefor in which the adsorbent properties of the ion-exchanger are involved, e.g. recovery of proteins or other high-molecular compounds
Systems and methods of providing run-time quality control and monitoring of a single or multiple sequencing runs are provided herein. In some embodiments, the run-time system includes or is in communication with a processor capable of determining various types of run-time information relating to the quality, progress, etc. of various sequencing runs. In some embodiments, the system can also be in communication with a user interface, for example, a GUI, capable of representing and communicating various types of information to a user regarding the quality of the individual or multiple runs, the functioning of the instrument, an error event, etc. Additionally, the system can capable of receiving actionable information from a user via the GUI thereby allowing the user to terminate or repeat various sequencing steps in a particular run, terminate a entire run, terminate all runs, allow a run to proceed, etc.
B24B 41/06 - Work supports, e.g. adjustable steadies
B24B 5/04 - Machines or devices designed for grinding surfaces of revolution on work, including those which also grind adjacent plane surfacesAccessories therefor involving centres or chucks for holding work for grinding cylindrical surfaces externally
Methods and systems for detecting a sample via optical pathways are described herein. In one aspect, a light detection system can include: a first optical pathway configured to direct emissions from an interrogation site to a first detector; a second optical pathway configured to direct emissions from the interrogation site to a second detector; and an automated switching module configured to receive a signal that induces the automated switching module to switch between (i) a first state that directs emissions from the interrogation site to the first optical pathway or a second state that directs emissions from the interrogation site to the second optical pathway and (ii) the other of the first state and the second state.
The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, methods provide for determining convergence of T cell receptor and/or B cell receptor repertoires in samples prior to a treatment and predicting a subject's response to the treatment based on the measured convergence frequency. In another aspect, methods provide for an immune receptor haplotype group and predicting a subject's potential or predisposition to be protected from or vulnerable to an adverse event following a treatment.
C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
C40B 30/04 - Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
Methods are provided for making bispecific antibodies and antibody conjugates comprising site-specifically cross-linking two or more antibodies, antibody fragments or Fc-fusion proteins. Also provided are compositions and uses for the bispecific antibodies and antibody conjugates. The bispecific antibodies may be used to treat a disease or condition. Also provided are methods for site-specifically conjugating a liposome, an mRNA or an siRNA to an antibody, and uses of the antibody-conjugated liposome, mRNA or siRNA.
A61K 9/00 - Medicinal preparations characterised by special physical form
A61K 47/60 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
A61K 47/68 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
A61K 47/69 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
C12N 9/24 - Hydrolases (3.) acting on glycosyl compounds (3.2)
C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
A method of applying a fluid composition to a flow cell of a sensor device includes inserting the sensor device into a holder; pipetting an aliquot of the fluid composition into a pipette tip; and applying the aliquot of the fluid composition to an opening of a fluidic connector engaged with the flow cell of the sensor device. The fluidic connector includes a body; and a plurality of fluidics interfaces formed in the body, each fluidic interface of the plurality of fluidics interfaces includes an opening, a first port in fluid communication with the opening, a second port, and a third port in fluidic communication with the second port.
A bioprocessing control system can include an intelligent gateway. The gateway can receive, from a controller, a request for data associated with the plurality of bioprocessing instruments including a primary data set and a secondary data set. The primary data set includes a block of primary data measured by the plurality of bioprocessing instruments. The secondary data set has a lower priority than the primary data set. The gateway can cause the controller to control the bioprocessing instrument by at least: sending, to the controller in response to the request, the block of primary data. The gateway can send, to the controller during the sending the block of primary data and in response to the request, a portion of the secondary data set, thereby reducing a load on the controller.
G05B 13/02 - Adaptive control systems, i.e. systems automatically adjusting themselves to have a performance which is optimum according to some preassigned criterion electric
H04L 12/66 - Arrangements for connecting between networks having differing types of switching systems, e.g. gateways
77.
EXTRACELLULAR VESICLE PROTEIN AND NUCLEIC ACID ANALYSIS
Methods of detecting one or more protein and one or more nucleic acid targets in EVs are provided. The methods include capturing EVs from a sample on a solid surface or bead to produce captured EVs, labeling the captured EVs with one or more detectably labeled antibodies, contacting the captured EVs with a fixation reagent, contacting the captured EVs with a permeabilization reagent, contacting the captured EVs with one or more nucleic acid detection reagents comprising a signal generating system, and detecting the one or more labeled antibodies and detecting a signal generated by the signal generating system, thereby detecting the one or more protein and one or more nucleic acid targets in the EVs.
The present invention relates to automated separation systems used for purifying multiphase fluid generated from bioreactors to prevent unintentional clogging of gas exhaust filters. In an embodiment, the separation system includes a separator assembly configured to generate a vortex motion for the multiphase fluid to efficiently break foam, release particle matter entrapped in the foam and release gases free of particle matter for passing through gas exhaust filters.
Systems and methods that enable analyte detection in a multiplexed amplification process can include obtaining, at multiple time points during the amplification process, composite fluorescence signal data associated with a composite fluorescence signal from at least a first probe type comprising a first fluorophore and a second probe type comprising a second fluorophore which has substantially overlapping spectral characteristics as said first fluorophore, the first probe type and the second probe type differing in thermal and/or temporal properties; and determining, based at least partially on the composite fluorescence signal data, fluorescence signal data associated with a fluorescence signal from a given probe type of the first probe type or the second probe type during the amplification process.
The instant technology generally relates to methods and compositions for expansion of mesenchymal stem cells in culture on a modified surface in the absence of a cell feeder layer and the absence of a cell adhesive coating on the surface. In some instances, the mesenchymal stem cells are expanded on the modified surface in a serum-free culture medium.
A fluid mixing system includes a collapsible container bounding a compartment and extending between a first end and an opposing second end. An elongated continuous drive line is at least partially disposed within the compartment of the container. A first portion of the drive line is laterally spaced apart from a second portion of the drive line, and the first portion of the drive line and the second portion of the drive line are rotatable within the compartment of the container.
C12M 1/06 - Apparatus for enzymology or microbiology with gas introduction means with agitator, e.g. impeller
B01F 27/1111 - Centrifugal stirrers, i.e. stirrers with radial outletsStirrers of the turbine type, e.g. with means to guide the flow with a flat disc or with a disc-like element equipped with blades, e.g. Rushton turbine
B01F 27/191 - Stirrers with two or more mixing elements mounted in sequence on the same axis with similar elements
B01F 35/41 - Mounting or supporting stirrer shafts or stirrer units on receptacles
B01F 35/513 - Flexible receptacles, e.g. bags supported by rigid containers
B01F 101/44 - Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
C12M 1/00 - Apparatus for enzymology or microbiology
C12M 1/02 - Apparatus for enzymology or microbiology with agitation meansApparatus for enzymology or microbiology with heat exchange means
C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
Disclosed are master mix compositions, kits, and related methods for use in amplifying a target nucleic acid. A master mix composition may be formulated to enable effective reaction mixture loading onto a dPCR plate, provide high proportion of valid/readable partitions, enable identification and distinguishing between amplified and unamplified partitions, enable detection of low abundance targets amidst high background levels of non-target nucleic acids and/or amidst inhibiting agents, and/or enable effective detection of low frequency mutant alleles.
A method of registering a location of a dispenser array in relation to a microfluidic array is provided. One of the dispenser array and the microfluidic array can be movable in relation to the frame, and the other can be fixed relative to the frame. Their relative positions can be identified by a set of coordinates. Identification of a fiducial marker can occur in a manner permitting the fiducial reference to appear in a first position of a field of view of a first camera when the dispenser array or the microfluidic array is in an alignment position. Quantities related to a vector displacement from the alignment position to a fixed position on the microfluidic array or the dispenser array can be identified. Quantities determined can be used to guide positioning of the dispenser array relative to the microfluidic array.
The instant technology relates to a production system to produce AAV vectors in a serum free suspension platform and at high titers. This technology uses reagents comprising media, cells, transfection reagent, AAV enhancer, and a lysis buffer, each of which is designed to provide maximal AAV production from suspension culture of mammalian cells, e.g. HEK293 cells. With this new system we are able to deliver up to about 2×1011 viral genomes per milliliter (vg/mL) of unconcentrated AAV vectors.
Purifying target biomolecules, such as nucleic acids or proteins, from a biological source is a time intensive process and is typically performed by a skilled technician or scientist owing to the highly technical nature of the work. Systems, devices, and methods disclosed herein enable the automated bioprocessing and purification of target biomolecules from a biological source. For example, an instrument and disposable cartridge are provided for automatedly isolating and purifying nucleic acids (such as plasmid DNA from a bacterial culture) or for isolating protein from any biological sample. Such an exemplary instrument and cartridge can work in concert to timely release, mix, and move the target biomolecule and various reagents and buffers through a target biomolecule purification process, resulting in a purified target biomolecule with less manual oversight than traditional approaches.
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
A method for nucleic acid sequencing includes receiving a plurality of signals indicative of a parameter measured for a plurality of defined spaces, at least some of the defined spaces including one or more sample nucleic acids, the signals being responsive to a plurality of nucleotide flows introducing nucleotides to the defined spaces; determining, for at least some of the defined spaces, whether the defined space includes a sample nucleic acid; processing, for at least some of the defined spaces determined to include a sample nucleic acid, the received signals to improve a quality of the received signals; and predicting a plurality of nucleotide sequences corresponding to respective sample nucleic acids for the defined spaces based on the processed signals and the nucleotide flows.
G01N 27/414 - Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
G01N 27/27 - Association of two or more measuring systems or cells, each measuring a different parameter, where the measurement results may be either used independently, the systems or cells being physically associated, or combined to produce a value for a further parameter
G16B 30/00 - ICT specially adapted for sequence analysis involving nucleotides or amino acids
The present disclosure relates to automated chromatography systems and methods for improving the accuracy of a variety of sensors in a chromatography system and measuring liquid and system parameters of a variety of liquids and bioprocessing equipment used to purify a target molecule. The automated chromatography systems and methods can run a startup operation to generate liquid and sensor parameter sets used in purification operations and recipes for purifying a target molecule in a chromatography column.
Disclosed are compositions, kits, and methods for quantifying a target nucleic acid from a sample. Compositions, kits, and methods enable the comparison of target nucleic acid loads between two or more test samples by normalizing measured levels (using a standard curve) of the target nucleic acid in each sample according to relative levels of endogenous nucleic acid in each test sample.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
The present disclosure relates to systems and methods for efficiently and easily implementing user-friendly purification operations and recipes to purify a target molecule by column chromatography. More specifically, the present application and disclosure relate to chromatography instrument control system modules, libraries and user interfaces used to control a chromatography instrument. The systems and methods are configured to provide a user with the ability to create, modify, recall, store, organize, prioritize, and run complex purification recipes to control a piece of chromatography equipment through a system controller and one or more graphical user interfaces.
A method for mixing a fluid includes: dispensing a first volume of a fluid into a flexible container, the flexible container being at least partially disposed within the chamber of a support housing; repeatedly moving the support housing and the flexible container contained therein so as to mix the first volume of fluid within the flexible container; adding further fluid into the flexible container after moving the support housing to form a second volume of fluid; and manipulating a mixing element within the flexible container so as to mix the second volume of fluid.
B01F 35/513 - Flexible receptacles, e.g. bags supported by rigid containers
B01F 27/07 - Stirrers characterised by their mounting on the shaft
B01F 27/213 - Mixers with rotary stirring devices in fixed receptaclesKneaders characterised by their rotating shafts characterised by the connection with the drive
B01F 27/807 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis wherein the stirrers or the receptacles are moved in order to bring them into operative positionMeans for fixing the receptacle with the stirrer-head pivoting about a horizontal axis to bring it in and out of operative position, e.g. with receptacles pivoting about a horizontal axis for emptying
B01F 27/88 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with a separate receptacle-stirrer unit that is adapted to be coupled to a drive mechanism
B01F 27/91 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with propellers
B01F 31/20 - Mixing the contents of independent containers, e.g. test tubes
B01F 31/23 - Mixing the contents of independent containers, e.g. test tubes by pivoting the containers about an axis
B01F 35/42 - Clamping or holding arrangements for mounting receptacles on mixing devices
B01F 35/43 - Supporting receptacles on frames or stands
Methods and systems are disclosed for computerized image processing for digital polymerase chain reaction (dPCR) analysis of a biological sample to automatically reject data of individual partitions from use in computing a target concentration result. Image data representing a plurality of partitions disposed in a container is obtained, including background image data captured prior to amplification cycles of a dPCR assay, and endpoint image data captured after amplification. Individual partition image data for the plurality of partitions is extracted from the image data. The partition background image data and the partition endpoint image data are pre-processed to obtain processed image data for input into one or more machine learning models, which are used to generate classification results. The one or more classification results are used to determine whether to reject image data corresponding to the partition of the plurality of partitions from use in computing the target concentration result.
Embodiments of a digital polymerase chain reaction (dPCR) system and method having improved auto-thresholding performance and accuracy are disclosed. One embodiment of the disclosure comprises a dPCR processing module including an auto-thresholding agent that dynamically analyzes and selects between Gaussian Mixture Modeling (GMM) and K-means clustering for use in auto-thresholding results of a dPCR assay using a dPCR instrument to improve dPCR measurement technology.
Various embodiments for improved dPCR systems and methods are disclosed. Some embodiments provide a multi-instrument dPCR system that facilitates analyzing samples together across multiple arrays of partitions, multiple sample plates, and/or multiple instruments to provide a better and higher throughput dPCR system. Aspects of various embodiments provide one or more of the following: Correction of illumination bias; quality control processing; flexible grouping of samples across partitions, sample plates, and instruments for thresholding and analysis; inter-instrument signal equalization for each dye channel to facilitate grouping samples across instruments; and improved auto-thresholding.
The present disclosure relates to automated chromatography systems and methods for improving the accuracy of a variety of sensors in a chromatography system and measuring liquid and system parameters of a variety of liquids and bioprocessing equipment used to purify a target molecule. The automated chromatography systems and methods can run a startup operation to generate liquid and sensor parameter sets used in purification operations and recipes for purifying a target molecule in a chromatography column.
Disclosed embodiments include a digital Polymerase chain reaction (dPCR) operative system having a method of digital image processing that corrects for illumination bias in data relating to an array of a sample plate comprising one or more arrays of sample partitions. In some embodiments, the method includes obtaining background image data corresponding to a digital image captured by the camera of the dPCR system after a background cycle of a dPCR assay. With respect to each partition, a local flat-fielding coefficient is derived and applied to the background image data to obtain local-bias-corrected background image data. Next, the local‑bias‑corrected background data is used to determine a set of global flat-fielding coefficients. The local and global flat-fielding coefficients are subsequently used to correct illumination bias endpoint image data to generate endpoint bias-corrected illumination values.
A computer-implemented method for visualizing dye interaction in a multiplexed biological sample is provided. The method includes receiving fluorescent emission data from each reaction site of a plurality of reaction sites. The reaction sites include at least a first, second, and third dye. The method further includes determining intensity values for at least a first, second, and third dye channel from the fluorescent emission data from each reaction site. The method further includes displaying, on a user interface, a first set of indications of a plurality of intensity values of the fluorescent emission data for the first, second, and third dye detected in the first dye channel, detected in the second dye channel, and detected in the third dye channel. The method further includes adjusting a label of the fluorescent emission data from a reaction site by comparing the first set of indications.
