Methods and systems for estimating gas supply pressure are described herein. The method can include measuring a runtime valve duty cycle for a valve of a gas chromatography system. The method can include estimating a runtime supply pressure from the runtime valve duty cycle and at least one of a runtime flow rate or downstream pressure based on one or more calibration valve duty cycles corresponding to one or more calibration supply pressures and one or more calibration flow rates. The method can include, responsive to determining the runtime supply pressure is greater than a threshold supply pressure value, generating a notification that the runtime supply pressure is greater than the threshold supply pressure value.
A column including particles having an average particle size ranging from about 1 μm to about 5 μm; the particles have an average pore size ranging from about 450 Å to about 3000 Å; and the particles have an average pore volume ranging from about 0.1 cm3/g to about 5 cm3/g is disclosed. The column can be a size exclusion chromatography column. The column can be used in a method to separate monomers from viral analytes, which is also disclosed.
A column including particles having an average particle size ranging from about 1 μm to about 5 μm; the particles have an average pore size ranging from about 450 Å to about 3000 Å; and the particles have an average pore volume ranging from about 0.1 cm3/g to about 5 cm3/g is disclosed. The column can be a size exclusion chromatography column. The column can be used in a method to separate monomers from viral analytes, which is also disclosed.
B01D 15/20 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives au conditionnement de la matière adsorbante ou absorbante
B01D 15/34 - Séparation par sélection en fonction de la taille, p. ex. chromatographie d'exclusion de tailleFiltration sur gelPerméation
B01J 20/28 - Compositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation caractérisées par leur forme ou leurs propriétés physiques
B01J 20/283 - Absorbants ou adsorbants poreux à base de silice
09 - Appareils et instruments scientifiques et électriques
Produits et services
Chromatography apparatus for laboratory use; Measuring apparatus; Measuring instruments; Testing apparatus not for medical purposes; Material testing instruments and machines; Liquid chromatography equipment comprising pumps, injectors, autosamplers, column-compartments and columns, detectors, valves and valve-actuators; Laboratory operating systems comprising computer hardware and computer software for use in connection with liquid chromatography systems; Liquid chromatographs for laboratory use; Detectors.
A composition, including an alkaloid, and a diaryl ketone is disclosed. Also, disclosed is a kit including one or more containers in which each of the one or more containers includes the composition. A method of using a standard composition is also disclosed.
A system for electronically and optically monitoring biological samples, the system including: a multi-well plate having a plurality of wells configured to receive a plurality of biological samples, each of the wells having a set of electrodes and a transparent window on a bottom surface of the well that is free of electrodes; an illumination module configured to illuminate the wells; a cradle configured to receive the multi-well plate, the cradle having an opening on the bottom that exposes the transparent windows of the wells; and an optical imaging module movable across different wells of a same multi-well plate to capture images through the windows.
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/32 - Inoculateur ou échantillonneur du type à champs multiples ou en continu
C12M 1/34 - Mesure ou test par des moyens de mesure ou de détection des conditions du milieu, p. ex. par des compteurs de colonies
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
A composition, including an alkaloid, and a diaryl ketone is disclosed. Also, disclosed is a kit including one or more containers in which each of the one or more containers includes the composition. A method of using a standard composition is also disclosed.
In some examples, a system may include at least two conveyors disposed at a specified distance apart and movable toward each other to reduce the specified distance or away from each other to increase the specified distance. The at least two conveyors may be operable to move a container that is contiguously disposable between the at least two conveyors along a first direction when the at least two conveyors are operated in the first direction and along a second direction that is opposite to the first direction when the at least two conveyors are operated in the second direction.
B65G 15/14 - Transporteurs comportant des surfaces porteuses de charges sans fin, c.-à-d. des tapis roulants ou autres systèmes équivalents, auxquelles l'effort de traction est transmis par des moyens différents des éléments d'entraînement sans fin de même configuration comprenant plusieurs surfaces sans fin travaillant ensemble et à axes parallèles longitudinaux, ou une multiplicité d'éléments parallèles, p. ex. des cordes formant une surface sans fin avec plusieurs courroies sans fin la charge étant transportée entre les courroies
10.
SYSTEM AND METHOD FOR VARIABLE SIZE CONTAINER HANDLING
In some examples, a system may include at least two conveyors disposed at a specified distance apart and movable toward each other to reduce the specified distance or away from each other to increase the specified distance. The at least two conveyors may be operable to move a container that is contiguously disposable between the at least two conveyors along a first direction when the at least two conveyors are operated in the first direction and along a second direction that is opposite to the first direction when the at least two conveyors are operated in the second direction.
B65C 9/04 - Dispositifs pour déplacer les objets, p. ex. des réceptacles, devant le poste d'étiquetage dotés de moyens pour faire tourner les objets
B65C 3/08 - Pose d'étiquettes sur des réceptacles courts et rigides sur le corps du réceptacle
B65G 15/14 - Transporteurs comportant des surfaces porteuses de charges sans fin, c.-à-d. des tapis roulants ou autres systèmes équivalents, auxquelles l'effort de traction est transmis par des moyens différents des éléments d'entraînement sans fin de même configuration comprenant plusieurs surfaces sans fin travaillant ensemble et à axes parallèles longitudinaux, ou une multiplicité d'éléments parallèles, p. ex. des cordes formant une surface sans fin avec plusieurs courroies sans fin la charge étant transportée entre les courroies
B65G 21/14 - Châssis ou carters de support ou de protection pour transporteurs sans fin ou éléments de traction des transporteurs à courroies ou à chaînes mobiles ou à pièces interchangeables ou relativement mobilesDispositifs pour déplacer le châssis ou des parties de celui-ci pour permettre le réglage de la longueur ou du profil des porte-charges ou de l'élément de traction
Methods and compositions are used for evaluating the performance of a mass spectrometer instrument. An oligonucleotide is introduced to the mass spectrometry instrument, the sample is analyzed in negative ionization mode, and the resulting mass spectrometry peaks are compared to known standards of the nucleotide-based sample for purposes of performing a qualification of the mass spectroscopy instrument in negative ionization mode. Compositions are provided for the methods and systems.
In some examples, an apparatus may include an ion injector including an ion injector entrance and an ion injector exit, and a capillary cap disposed at the ion injector exit. The capillary cap may include a capillary cap exit opening that is shaped and sized to reduce gas turbulence after the ion injector exit and improve ion signal stability.
H01J 37/317 - Tubes à faisceau électronique ou ionique destinés aux traitements localisés d'objets pour modifier les propriétés des objets ou pour leur appliquer des revêtements en couche mince, p. ex. implantation d'ions
The disclosure provides methods for aligning a portion of a first image of a tissue with a portion of a second image of the tissue by obtaining one or more local transformations based on the portion of the first image and/or the portion of the second image, wherein each of the one or more local transformations is: (i) for aligning a patch of the first image to a patch of the second image matching the patch of the first image, and (ii) associated with the patch of the first image and/or with the patch of a second image; and determining a transformation for aligning the portion of the first image to the portion of the second image according to the one or more local transformations. Moreover, a corresponding device and software implementing functionality of the methods is provided.
14.
ANTI-HUMAN CD10 ANTIBODIES FOR USE IN IMMUNOHISTOCHEMISTRY (IHC) PROTOCOLS TO DIAGNOSE CANCER
In alternative embodiments, provided are non-natural synthetic antibodies capable of specifically binding a human CD10 polypeptide, or neprilysin, polypeptide. In alternative embodiments, also provided are products of manufacture and kits comprising antibodies as provided herein, and methods for making and using antibodies as provided herein, where the antibodies can be used for in vitro diagnostics by immunohistochemistry (IHC). In alternative embodiments, antibodies as provided herein are used in IHC protocols to diagnose a cancer, for example, leukemic cell cancer of pre-B phenotype, acute lymphoblastic leukemia (ALL), angioimmunoblastic T cell lymphoma, Burkitt lymphoma, chronic myelogenous leukemia in blast crisis, diffuse large B-cell lymphoma, hairy cell leukemia, myeloma, precursor B lymphoblastic leukemia or lymphoma, follicular lymphoma, mantle cell lymphoma, precursor T lymphoblastic leukemia or lymphoma, non-hematolymphoid sarcoma, or carcinoma such as renal cell carcinoma or metaplastic breast carcinoma.
G01N 33/574 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour le cancer
C07K 16/28 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains contre des récepteurs, des antigènes de surface cellulaire ou des déterminants de surface cellulaire
09 - Appareils et instruments scientifiques et électriques
Produits et services
Mass detection systems; instruments for mass spectrometry measurements; liquid chromatography mass spectrometry systems; liquid chromatography mass detection systems; scientific instruments, namely, liquid chromatography mass spectrometers, including liquid delivery system (pump), injection system, autosampler, column compartment, valving, ion source generation, and calibrant delivery systems; software for instrument control, instrument tuning, signal collection, data analysis, and reporting of the output of liquid chromatography mass spectrometers; liquid chromatography mass spectrometry detection systems for biological and chemical separations, measurements, screening, targeted analysis, and identification
09 - Appareils et instruments scientifiques et électriques
Produits et services
Mass detection systems; instruments for mass spectrometry measurements; liquid chromatography mass spectrometry systems; liquid chromatography mass detection systems; scientific instruments, namely, liquid chromatography mass spectrometers, including liquid delivery system (pump), injection system, autosampler, column compartment, valving, ion source generation, and calibrant delivery systems; software for instrument control, instrument tuning, signal collection, data analysis, and reporting of the output of liquid chromatography mass spectrometers; liquid chromatography mass spectrometry detection systems for biological and chemical separations, measurements, screening, targeted analysis, and identification
The invention relates to a fluid valve (100) for an analyzer (10), comprising: i) a stator component (110) comprising: a first port (150) in a first radius (111) and a plurality of second ports (114) along a second radius (112); and ii) a rotor component (120) comprising: a slot (121) which is coupled to the first port (150), wherein the slot (121) comprises: a tangential part (122) which extends along a segment of the second radius (112). a) the slot (121) also comprises a radial part (125) which extends from the first port (150) to the second radius (112), and/or b) the fluid valve (100) is designed to rotate the rotor component (120) in relation to the stator component (110) in such a way that mixing and/or proportioning fluid, in particular solvent (113, 115), is enabled.
G01N 30/20 - Injection utilisant une valve d'échantillonnage
G01N 30/34 - Contrôle des paramètres physiques du fluide vecteur de la composition du fluide, p. ex. du gradient
F16K 11/074 - Soupapes ou clapets à voies multiples, p. ex. clapets mélangeursRaccords de tuyauteries comportant de tels clapets ou soupapesAménagement d'obturateurs et de voies d'écoulement spécialement conçu pour mélanger les fluides dont toutes les faces d'obturation se déplacent comme un tout comportant uniquement des tiroirs à éléments de fermeture articulés à pivot à faces d'obturation planes
18.
SAMPLE INJECTOR WITH FLOATING NEEDLE SEAT FOR AN ANALYTICAL DEVICE
A sample injector for an analytical device includes a needle seat configured to receive a needle for injecting a sample into an injector path. The needle seat is mounted in a floating manner so that the orientation of the needle seat is aligned automatically with respect to the orientation of the approaching needle. The sample injector may be provided in an analytical device such as a chromatographic device.
An apparatus for containing a three-dimensional cellular material surrounded by a medium that facilitates and maintains the centering of the three-dimensional cellular material throughout an assay is provided. The apparatus includes a well having an open proximal end and a closed distal end. Further, the well defines a compartment having an interior surface and a sample nesting site for containing the three-dimensional cellular material surrounded by the medium. A central indentation is located at the closed distal end of the well, a first concentric lip is located above the central indentation in a y-direction towards the open proximal end of the well, and a second concentric lip is located above the first concentric lip in the y-direction towards the open proximal end of the well. Additionally, the first concentric lip and the second concentric lip define a groove therebetween. A method of forming a three-dimensional cellular material is also provided.
A magnetic coupling assembly includes a thermal isolation system that isolates the assembly from a heat source such as a pump coupled to a driven side of the assembly, thereby enabling the assembly to operate at a reduced temperature. The system may include a labyrinth seal that reduces convective heat transfer to the assembly. The seal may include a sealing gap defined between a rotating component and a stationary component. The gap is sized to limit gas conductance therethrough. The system may also include one or more spacers positioned to reduce or eliminate conductive heat transfer from one component to another. The assembly may be utilized in a pump for contactless coupling of a motor shaft to a pump shaft.
H02K 49/10 - Embrayages dynamo-électriquesFreins dynamo-électriques du type à aimant permanent
F04C 2/02 - Machines ou pompes à piston rotatif du type à engrènement, c.-à-d. avec un mouvement de translation circulaire des organes coopérants, chaque organe possédant le même nombre de dents ou de parties équivalentes de prise
F04C 18/02 - Pompes à piston rotatif spécialement adaptées pour les fluides compressibles du type à engrènement, c.-à-d. avec un mouvement de translation circulaire des organes coopérants, chaque organe possédant le même nombre de dents ou de parties équivalentes de prise
F04C 29/00 - Parties constitutives, détails ou accessoires de pompes ou d'installations de pompage spécialement adaptées pour les fluides compressibles non couverts dans les groupes
H02K 5/18 - Enveloppes ou enceintes caractérisées par leur configuration, leur forme ou leur construction avec des nervures ou des ailettes pour améliorer la transmission de la chaleur
21.
Chemically Modified Guide RNAs for CRISPR/CAS-Mediated Gene Correction
The Board of Trustees of the Leland Stanford Junior University (USA)
Agilent Technologies, Inc. (USA)
Inventeur(s)
Porteus, Matthew H.
Hendel, Ayal
Clark, Joe
Bak, Rasmus O.
Ryan, Daniel E.
Dellinger, Douglas J.
Kaiser, Robert
Myerson, Joel
Abrégé
Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a modified sgRNA to correct a mutation in a target gene associated with the genetic disease.
A capillary array assembly is fabricated by inserting capillaries into respective grooves of a substrate and fixing the capillaries to the substrate. The capillaries are fixed by applying heat and force to the substrate, with a defined displacement of substrate material and for a defined period of time, thereby spreading heated portions of the substrate over the capillaries. After applying the heat and force, capillaries are at least partially embedded by the substrate and may be at least partially exposed to a top outside surface of the substrate by respective slots. The capillary array assembly may be utilized, for example, in a sample analysis instrument such as a capillary electrophoresis (CE) instrument.
The invention relates to a temperature control apparatus (100) for an analysis device (10), having: i) a receiving volume (110) for receiving at least one sample container (130); and ii) a temperature control element (120) which is designed to at least partially control the temperature of the at least one sample container (130) in the receiving volume (110); wherein the temperature control apparatus (100) is designed to switch between: a) a first operating mode for controlling the temperature of the at least one sample container (130) by means of the temperature control element (120), wherein the temperature control element (120) is designed to directly contact the at least one sample container (130) in the first operating mode, wherein the direct contact comprises: forming an integral connection and/or controlling the temperature by means of a temperature control medium (125) which flows through the temperature control element (120); b) a second operating mode for not controlling the temperature of the at least one sample container (130) by means of the temperature control element (120).
In some examples, a three-dimensional printed nanospray interface for mass spectrometry may include a body including a sheath inlet connected to a sheath outlet for passage of sheath liquid to an emitter. The emitter may include a sidewall including a stepped texture on an inner surface of the sidewall.
In some examples, a three-dimensional printed nanospray interface for mass spectrometry may include a body including a sheath inlet connected to a sheath outlet for passage of sheath liquid to an emitter. The emitter may include a sidewall including a stepped texture on an inner surface of the sidewall.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
B33Y 80/00 - Produits obtenus par fabrication additive
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Custom manufacturing services for others in the field of
drugs for therapeutic use in humans, nucleic acids, and
nucleic acid materials; consulting for custom manufacturing
process; technical support services, namely, providing
technical advice related to the custom manufacture of drugs
for therapeutic use in humans, nucleic acids, and nucleic
acid materials. Pharmaceutical drug development services; biochemical
research and analysis, namely, analysis of nucleic acids;
scientific study and research in the field of nucleic acids
and nucleic acid materials; quality management services,
namely, quality evaluation and analysis, quality assurance,
and quality control, in the field of pharmaceutical drugs
for therapeutic use in humans.
27.
IMMUNOHISTOCHEMISTRY (IHC) PROTOCOLS AND METHODS FOR DIAGNOSING AND TREATING CANCER - CLAUDIN 18.2
In alternative embodiments, provided are immunohistochemistry (IHC) methods for determining and scoring reproducibly the extent of expression of the protein Claudin 18.2, in a tissue sample. In alternative embodiments, provided are methods for diagnosing, treating or ameliorating or assessing the risk of recurrence for a cancer or a tumor using an IHC method as provided herein. In alternative embodiments, provided are kits comprising components and instructions for practicing methods as provided herein. The present application describes methods for scoring Claudin 18.2 expression and utilizing the score as a companion or complementary diagnostic or to treat or ameliorate cancer or a tumor.
G01N 33/574 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour le cancer
C07K 16/18 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains
A61K 39/395 - AnticorpsImmunoglobulinesImmunsérum, p. ex. sérum antilymphocitaire
C07K 14/47 - Peptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'animauxPeptides ayant plus de 20 amino-acidesGastrinesSomatostatinesMélanotropinesLeurs dérivés provenant d'humains provenant de vertébrés provenant de mammifères
40 - Traitement de matériaux; recyclage, purification de l'air et traitement de l'eau
42 - Services scientifiques, technologiques et industriels, recherche et conception
Produits et services
Custom manufacturing services for others in the field of
drugs for therapeutic use in humans, nucleic acids, and
nucleic acid materials; consulting for custom manufacturing
process; technical support services, namely, providing
technical advice related to the custom manufacture of drugs
for therapeutic use in humans, nucleic acids, and nucleic
acid materials. Pharmaceutical drug development services; biochemical
research and analysis, namely, analysis of nucleic acids;
scientific study and research in the field of nucleic acids
and nucleic acid materials; quality management services,
namely, quality evaluation and analysis, quality assurance,
and quality control, in the field of pharmaceutical drugs
for therapeutic use in humans.
In some examples, an apparatus may include an advection flow structure including a passage to transport, via advection by a gas flow, an ablated sample from an ablation region to an ionization region.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/26 - Spectromètres de masse ou tubes séparateurs de masse
H01J 49/24 - Systèmes à vide, p. ex. maintenant des pressions voulues
The present invention relates to methods for construction of pharmamers i.e. vaccine components characterized by their multimerization domain and the attached biologically active molecules, and their use in preparation of vaccines that contains the pharmamers alone or in combination with other molecules. The individual molecules of the construct can be bound to each other or the multimerization domain(s) by covalent or non-covalent bonds, directly or via linkers. The invention further relates to the use of such preparations in vaccine settings aimed to function as preventive/prophylactic or therapeutic vaccines in humans and animals.
A61K 39/21 - Retroviridae, p. ex. virus de l'anémie infectieuse équine
A61K 47/61 - Préparations médicinales caractérisées par les ingrédients non actifs utilisés, p. ex. les supports ou les additifs inertesAgents de ciblage ou de modification chimiquement liés à l’ingrédient actif l’ingrédient non actif étant chimiquement lié à l’ingrédient actif, p. ex. conjugués polymère-médicament l’ingrédient non actif étant un agent de modification l’agent de modification étant un composé organique macromoléculaire, p. ex. une molécule oligomérique, polymérique ou dendrimérique le composé organique macromoléculaire étant un polysaccharide ou l’un de ses dérivés
A61K 47/64 - Conjugués médicament-peptide, médicament-protéine ou médicament-acide polyaminé, c.-à-d. l’agent de modification étant un peptide, une protéine ou un acide polyaminé lié par covalence ou complexé à un agent thérapeutiquement actif
In some examples, an apparatus may include an advection flow structure including a passage to transport, via advection by a gas flow, an ablated sample from an ablation region to an ionization region.
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
H01J 49/16 - Sources d'ionsCanons à ions utilisant une ionisation de surface, p. ex. émission thermo-ionique ou photo-électrique
H01J 49/24 - Systèmes à vide, p. ex. maintenant des pressions voulues
The present invention is directed to a sample holder for use in molecular absorption spectroscopy. The sample holder comprises a first surface having a first predetermined geometry. and a second surface having a second predetermined geometry. The first surface is opposite the second surface. The sample holder is configured to hold a measurement sample between the first surface and the second surface such that a distance between the first surface and the second surface defines an optical pathlength of the sample holder. The predetermined geometries of the first surface and the second surface provides a continuously variable cross-section across the sample holder so as to provide a continuous range of optical pathlengths.
G01N 21/31 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en recherchant l'effet relatif du matériau pour les longueurs d'ondes caractéristiques d'éléments ou de molécules spécifiques, p. ex. spectrométrie d'absorption atomique
33.
Smartphone display screen or portion thereof with transitional graphical user interface
09 - Appareils et instruments scientifiques et électriques
Produits et services
Computer software, namely, downloadable and recorded
software for collecting, processing, integrating,
organizing, analyzing, visualizing, and communicating
scientific data in the field of mass spectrometry.
Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as a rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.
C12N 9/08 - Oxydoréductases (1.), p. ex. luciférase agissant sur le peroxyde d'hydrogène comme accepteur (1.11)
C12N 9/96 - Stabilisation d'une enzyme par formation d'un adduct ou d'une compositionFormation de conjugaisons d'enzymes
C12Q 1/28 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir une oxydoréductase une peroxydase
G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
A cell filtration device and method use a negative dielectrophoretic force (nDEP) to repulse cells from pores of an insulating membrane. A cell suspension is introduced to a first fluidic region, and a fluid and other components pass through pores of the insulating membrane to a second fluidic region. Cells are retained in the first fluidic region and can be recovered continuously or at intervals. The cell filtration device and method minimize contact of the insulating membrane by the cells and avoids clogging of pores.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
In alternative embodiments, provided are immunohistochemistry (IHC) methods for determining and scoring reproducibly the extent of expression of the protein Melanoma Associated Antigen Gene-A4 (MAGE-A4), in a tissue sample. In alternative embodiments, provided are methods for diagnosing, treating or ameliorating or assessing the risk of recurrence for a cancer or a tumor using an IHC method as provided herein. In alternative embodiments, provided are kits comprising components and instructions for practicing methods as provided herein. The present application describes methods for scoring MAGE-A4 expression and utilizing the score as a companion or complementary diagnostic or to treat or ameliorate cancer or a tumor.
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
C07K 16/30 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains contre des récepteurs, des antigènes de surface cellulaire ou des déterminants de surface cellulaire provenant de cellules de tumeurs
G01N 1/28 - Préparation d'échantillons pour l'analyse
A capillary array assembly (100) includes a capillary array window holder (104) that provides multiple capillary channels. A section of each capillary channel is an open channel (678) that is exposed to excitation light on at least one side of the capillary array assembly (100). Adjacent open channels (678) are separated from each other by window bars (156). Multiple capillaries (108) are disposed in respective capillary channels, such that windows (148) of the capillaries (108) are located in the open channels (678). The window bars (156) block line of sight between adjacent capillary windows (148) to reduce or eliminate cross-talk between adjacent capillaries (108) when making optical-based measurements of the samples. The capillary array assembly (100) may be mounted in a sample analysis system (1800), which may for example be configured to perform capillary electrophoresis on the samples.
A solvent supply system supplies solvent for one or more chromatographic separation systems each including one or more input channels. Each input channel is configured for fluidically coupling with a respective solvent container for supplying the respective input channel with a respective solvent from the respective solvent container. The solvent supply system includes a solvent identification unit configured for providing a channel identification for identifying a specific solvent container to supply a specific input channel of a specific chromatographic separation system, and for assigning the channel identification to the specific solvent container.
B01D 15/20 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives au conditionnement de la matière adsorbante ou absorbante
B01D 15/18 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives aux différents types d'écoulement
40.
DEPARAFFINIZATION OF TISSUE UTILIZING ELECTRIC FIELD
Paraffin-embedded tissue is prepared removing paraffin from the tissue. The paraffin is removed by generating an electric field effective to produce plasma and direct charged species of the plasma to the paraffin, thereby rendering the paraffin responsive to the electric field. The electric field may move the paraffin out from the tissue due to electrostatic force. Movement of the paraffin may be assisted by moving an electrode utilized to generate the electric field relative to the paraffin. Movement of the paraffin also may be assisted by applying a solvent and/or heat energy to the tissue.
Provided herein is an ion source containing a plurality of components, at least one of which is partially coated with a layer of silicon. The ion source reduces reactivity between the sample and the carrier gas, reduces or eliminates tailing in ion chromatograms, and/or improves mass spectral fidelity. Also provided are methods of using the ion source in a mass spectrometer or gas chromatograph-mass spectrometer.
A flame-based detector (102) comprises a housing (104), a burner (106), a fuel flow path (108), an air flow path (110), a collector (114), an ignitor (116), an ignition promoter port (122), and an exhaust vent (128). The ignition promoter port (122) introduces a gas close to the ignitor (116), thereby facilitating combustion.
In alternative embodiments, provided are immunohistochemistry (IHC) methods and kits for determining and scoring reproducibly the extent of expression of the protein tyrosine-protein kinase-like 7 (PTK7), also known as: colon carcinoma kinase 4 (CCK4); HER2 or receptor tyrosine-protein kinase erbB-2, or cluster of differentiation 340 (CD340); programmed death-ligand 1 (PD-L1), or cluster of differentiation 274 (CD274); B7 homolog 1 (B7-H1); and, Ki-67 or MKI67 (Marker of Proliferation Ki-67), in a tissue sample. In alternative embodiments, provided are methods and kits for diagnosing or selecting an individual eligible for treatment with a cancer or tumor therapeutic, or assessing the risk of recurrence for a cancer or a tumor using an IHC method as provided herein. In alternative embodiments, provided are kits comprising components and instructions for practicing methods as provided herein. The present application describes methods for scoring PTK7 expression and utilizing the score as a companion or complementary diagnostic, or to aid the treatment or amelioration of a cancer or a tumor.
G01N 33/574 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour le cancer
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
Flame-based detectors having an ignitor are protected from corrosion by flowing a protective gas over the ignitor. The protective gas layer protects the ignitor from corrosive components in exhaust gas.
The current technology is related to methods for rapidly determining the metabolic baseline and potential of living cells. Embodiments relate to measuring the activity of each of the two major energy-generating pathways within the cell: mitochondrial respiration and glycolysis, first under baseline conditions, and again after applying a stress to the cells to demand increased energy supply. In some embodiments the stress may be applied by exposing the cells to a combination of two chemical compounds: a mitochondrial uncoupler and an ATP synthase inhibitor. In one embodiment, the metabolic energy generating activity of the mitochondrial respiration pathway is determined by measuring the rate of oxygen consumption by the living cells, and the metabolic energy generating activity of the glycolysis pathway is determined from a measurement of extracellular acidification caused by secretion of protons from the cell. Other embodiments are related to an apparatus for determining a metabolic potential of a cell sample in a well of a multiwell plate.
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
47.
APPARATUS AND METHODS FOR BAKING TISSUE SLIDES IN A SLIDE BASKET
Specimens in an embedding medium are attached to slides by heating (e.g., baking). Slides with the tissue sections or other embedded specimens are inserted in a slide basket which holds them vertically. The slide basket is placed into a heating chamber which has a sorbent support.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 1/36 - Enrobage ou montage analogue d'échantillons
In alternative embodiments, provided are immunohistochemistry (IHC) methods for determining and scoring reproducibly the extent of nuclear expression of protein Ki-67 (also known as MK167) in a tissue sample. In alternative embodiments, provided are methods for diagnosing, treating or ameliorating or assessing the risk of recurrence for a cancer or a tumor using an IHC method as provided herein. In alternative embodiments, provided are kits comprising components and instructions for practicing methods as provided herein.
G01N 33/574 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour le cancer
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
49.
POLYMERASE MUTANTS AND USE WITH 3'-OH UNBLOCKED REVERSIBLE TERMINATORS
Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3′—OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.
A device for determining a target separation method for separating a fluidic sample by a target sample separation apparatus modifies an initial separation method for an initial sample separation apparatus. The device includes a data provision unit configured for providing a first initial data set characterizing the initial separation method, a second initial data set characterizing properties of the target sample separation apparatus, a third initial data set characterizing properties of the fluidic sample, a fourth initial data set characterizing properties of the initial sample separation apparatus, and a determining unit configured for determining a target data set characterizing the target separation method by carrying out a numerical analysis based on the first initial data set, the second initial data set, the third initial data set, and the fourth initial data set.
The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.
B01D 15/22 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives à la structure de la colonne
C23C 16/04 - Revêtement de parties déterminées de la surface, p. ex. au moyen de masques
C23C 16/44 - Revêtement chimique par décomposition de composés gazeux, ne laissant pas de produits de réaction du matériau de la surface dans le revêtement, c.-à-d. procédés de dépôt chimique en phase vapeur [CVD] caractérisé par le procédé de revêtement
C23C 16/455 - Revêtement chimique par décomposition de composés gazeux, ne laissant pas de produits de réaction du matériau de la surface dans le revêtement, c.-à-d. procédés de dépôt chimique en phase vapeur [CVD] caractérisé par le procédé de revêtement caractérisé par le procédé utilisé pour introduire des gaz dans la chambre de réaction ou pour modifier les écoulements de gaz dans la chambre de réaction
52.
SYSTEMS AND METHODS FOR TARGETED NUCLEIC ACID CAPTURE
The present disclosure provides systems and methods for targeted indirect, synergistic hybridization capture of a template for amplification and analysis of target sequences. The captured templates can be further treated with bisulfite or other methylation reagents to study the methylation pattern of the nucleic acid molecules of the template.
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p. ex. séquençage par hybridation [SBH]
C12Q 1/6806 - Préparation d’acides nucléiques pour analyse, p. ex. pour test de réaction en chaîne par polymérase [PCR]
C12Q 1/6818 - Tests d’hybridation caractérisés par les moyens de détection impliquant l’interaction de plusieurs marqueurs, p. ex. transfert d’énergie de résonance
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
In some examples, an electron capture dissociation (ECD) cell may include a plurality of lenses. At least one lens of the plurality of lenses may be asymmetrically arranged about an axis that is orthogonal to a central axis of the ECD cell and intersects a center-point along a length of the ECD cell defined along the central axis.
The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.
B01D 15/22 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives à la structure de la colonne
C23C 16/04 - Revêtement de parties déterminées de la surface, p. ex. au moyen de masques
C23C 16/44 - Revêtement chimique par décomposition de composés gazeux, ne laissant pas de produits de réaction du matériau de la surface dans le revêtement, c.-à-d. procédés de dépôt chimique en phase vapeur [CVD] caractérisé par le procédé de revêtement
C23C 16/455 - Revêtement chimique par décomposition de composés gazeux, ne laissant pas de produits de réaction du matériau de la surface dans le revêtement, c.-à-d. procédés de dépôt chimique en phase vapeur [CVD] caractérisé par le procédé de revêtement caractérisé par le procédé utilisé pour introduire des gaz dans la chambre de réaction ou pour modifier les écoulements de gaz dans la chambre de réaction
55.
METAL COMPONENTS WITH INERT VAPOR PHASE COATING ON INTERNAL SURFACES
The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.
B01D 15/22 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives à la structure de la colonne
C23C 16/04 - Revêtement de parties déterminées de la surface, p. ex. au moyen de masques
C23C 16/44 - Revêtement chimique par décomposition de composés gazeux, ne laissant pas de produits de réaction du matériau de la surface dans le revêtement, c.-à-d. procédés de dépôt chimique en phase vapeur [CVD] caractérisé par le procédé de revêtement
C23C 16/455 - Revêtement chimique par décomposition de composés gazeux, ne laissant pas de produits de réaction du matériau de la surface dans le revêtement, c.-à-d. procédés de dépôt chimique en phase vapeur [CVD] caractérisé par le procédé de revêtement caractérisé par le procédé utilisé pour introduire des gaz dans la chambre de réaction ou pour modifier les écoulements de gaz dans la chambre de réaction
56.
METAL COMPONENTS WITH INERT VAPOR PHASE COATING ON INTERNAL SURFACES
The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.
B01D 15/22 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives à la structure de la colonne
C23C 16/04 - Revêtement de parties déterminées de la surface, p. ex. au moyen de masques
C23C 16/44 - Revêtement chimique par décomposition de composés gazeux, ne laissant pas de produits de réaction du matériau de la surface dans le revêtement, c.-à-d. procédés de dépôt chimique en phase vapeur [CVD] caractérisé par le procédé de revêtement
C23C 16/455 - Revêtement chimique par décomposition de composés gazeux, ne laissant pas de produits de réaction du matériau de la surface dans le revêtement, c.-à-d. procédés de dépôt chimique en phase vapeur [CVD] caractérisé par le procédé de revêtement caractérisé par le procédé utilisé pour introduire des gaz dans la chambre de réaction ou pour modifier les écoulements de gaz dans la chambre de réaction
57.
METAL COMPONENTS WITH INERT VAPOR PHASE COATING ON INTERNAL SURFACES
The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.
B01D 15/22 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives à la structure de la colonne
C23C 16/04 - Revêtement de parties déterminées de la surface, p. ex. au moyen de masques
C23C 16/44 - Revêtement chimique par décomposition de composés gazeux, ne laissant pas de produits de réaction du matériau de la surface dans le revêtement, c.-à-d. procédés de dépôt chimique en phase vapeur [CVD] caractérisé par le procédé de revêtement
C23C 16/455 - Revêtement chimique par décomposition de composés gazeux, ne laissant pas de produits de réaction du matériau de la surface dans le revêtement, c.-à-d. procédés de dépôt chimique en phase vapeur [CVD] caractérisé par le procédé de revêtement caractérisé par le procédé utilisé pour introduire des gaz dans la chambre de réaction ou pour modifier les écoulements de gaz dans la chambre de réaction
58.
FLUID SUPPLY DEVICES FOR FORMING A FILTERED MOBILE PHASE FOR A SAMPLE SEPARATING DEVICE
A fluid supply device for providing a mobile phase for a sample separating device includes a supply path configured to provide a fluid for forming at least a part of the mobile phase, a fluid conveying unit configured to receive and pressurize the fluid from the supply path and to convey the fluid to the sample separating device, and a sterile filter disposed in the supply path upstream of the fluid conveying unit and configured to filter the fluid. The fluid supply device may include a pre-filter pump configured to push the fluid through the sterile filter and/or a buffer unit configured to buffer the fluid.
The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.
B01D 15/22 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives à la structure de la colonne
C23C 16/04 - Revêtement de parties déterminées de la surface, p. ex. au moyen de masques
C23C 16/44 - Revêtement chimique par décomposition de composés gazeux, ne laissant pas de produits de réaction du matériau de la surface dans le revêtement, c.-à-d. procédés de dépôt chimique en phase vapeur [CVD] caractérisé par le procédé de revêtement
C23C 16/455 - Revêtement chimique par décomposition de composés gazeux, ne laissant pas de produits de réaction du matériau de la surface dans le revêtement, c.-à-d. procédés de dépôt chimique en phase vapeur [CVD] caractérisé par le procédé de revêtement caractérisé par le procédé utilisé pour introduire des gaz dans la chambre de réaction ou pour modifier les écoulements de gaz dans la chambre de réaction
60.
METAL COMPONENTS WITH INERT VAPOR PHASE COATING ON INTERNAL SURFACES
The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.
B01D 15/22 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives à la structure de la colonne
C23C 16/04 - Revêtement de parties déterminées de la surface, p. ex. au moyen de masques
C23C 16/44 - Revêtement chimique par décomposition de composés gazeux, ne laissant pas de produits de réaction du matériau de la surface dans le revêtement, c.-à-d. procédés de dépôt chimique en phase vapeur [CVD] caractérisé par le procédé de revêtement
C23C 16/455 - Revêtement chimique par décomposition de composés gazeux, ne laissant pas de produits de réaction du matériau de la surface dans le revêtement, c.-à-d. procédés de dépôt chimique en phase vapeur [CVD] caractérisé par le procédé de revêtement caractérisé par le procédé utilisé pour introduire des gaz dans la chambre de réaction ou pour modifier les écoulements de gaz dans la chambre de réaction
61.
METAL COMPONENTS WITH INERT VAPOR PHASE COATING ON INTERNAL SURFACES
The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.
B01D 15/22 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement relatives à la structure de la colonne
C23C 16/04 - Revêtement de parties déterminées de la surface, p. ex. au moyen de masques
C23C 16/44 - Revêtement chimique par décomposition de composés gazeux, ne laissant pas de produits de réaction du matériau de la surface dans le revêtement, c.-à-d. procédés de dépôt chimique en phase vapeur [CVD] caractérisé par le procédé de revêtement
C23C 16/455 - Revêtement chimique par décomposition de composés gazeux, ne laissant pas de produits de réaction du matériau de la surface dans le revêtement, c.-à-d. procédés de dépôt chimique en phase vapeur [CVD] caractérisé par le procédé de revêtement caractérisé par le procédé utilisé pour introduire des gaz dans la chambre de réaction ou pour modifier les écoulements de gaz dans la chambre de réaction
62.
DEPARAFFINIZATION OF TISSUE BY ELECTRIC FIELD GENERATION AND IONIZATION
Paraffin-embedded tissue, which may be disposed on a solid substrate, is prepared by a dry technique that removes paraffin from tissue without adding any liquid to the tissue, thereby rendering the tissue substantially free of paraffin. The dry technique may entail applying heat energy to the tissue effective to melt the paraffin and thereby render it flowable, and applying an electric field. The electric field is effective to impart electrical charge to the paraffin and to move the paraffin out from the tissue due to electrical charge repulsion or attraction, which may be assisted by moving an electrode utilized to generate the electric field relative to the paraffin. The electric field, or both the electric field and the heat energy, may be applied until the tissue is substantially free of paraffin.
A sample handling device for handling a sample container, in particular for an analysis device for analyzing a fluidic sample in the sample container, includes: i) a push-push mechanism for fixing and releasing the sample container; and ii) a coupling region for releasably coupling to a sample moving device. The sample handling device is a passive device which is adapted to perform the fixing and the releasing free of a drive, in particular a motor. Furthermore, a related sample handling arrangement, an analysis device, and a method are described.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 35/02 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet en utilisant une série de récipients à échantillons déplacés par un transporteur passant devant un ou plusieurs postes de traitement ou d'analyse
64.
Visual Interface for Generation of Groups and Experiment Building
A user interface that includes a plate map that automatically updates to provide indicia descriptive of parameter assignments and parameter groups can provide an intuitive system for building experiments. For example, a user may provide one or more inputs to generate and assign a parameter setting to a plurality of cells that represent a plurality of wells. The user interface may update the plate map to depict one or more indicia to indicate which wells have been assigned the new parameter setting.
G16H 10/40 - TIC spécialement adaptées au maniement ou au traitement des données médicales ou de soins de santé relatives aux patients pour des données relatives aux analyses de laboratoire, p. ex. pour des analyses d’échantillon de patient
Disclosed is an analytical device (100), comprising: i) a fluid compartment (120), configured to accommodate a solvent; ii) a blocking device (125), configured to close an input (121) and/or an output (122) of the fluid compartment (120); iii) a flow sensor (110) and/or a pressure sensor, coupled to the fluid compartment (120), and configured to perform a measurement with respect to the solvent; iv) a temperature change device (130), coupled to the fluid channel (120), and configured to perform a temperature change with respect to the fluid compartment (120); and v) a determination device, configured to determine a thermal property of the solvent based on the measurement and the temperature change.
A substrate orientation device includes a frame that supports a substrate on which liquid is dispensed, for example as may relate to (bio)chemical microarray fabrication. The frame is movable to different positions, such as for mounting the substrate to the frame, depositing droplets on the substrate, and applying a bulk liquid to the substrate. The substrate orientation device may be movable to different locations, such as different liquid dispensing devices, for example a printer and a flow cell.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
67.
IMMUNOHISTOCHEMISTRY (IHC) MAGE-A4 SCORING PROTOCOLS AND METHODS FOR AIDING CANCER TREATMENTS
In alternative embodiments, provided are immunohistochemistry (IHC) methods for determining and scoring reproducibly the extent of expression of the protein Melanoma Associated Antigen Gene-A4 (MAGE-A4), in a tissue sample. In alternative embodiments, provided are methods for diagnosing, treating or ameliorating or assessing the risk of recurrence for a cancer or a tumor using an IHC method as provided herein. In alternative embodiments, provided are kits comprising components and instructions for practicing methods as provided herein. The present application describes methods for scoring MAGE-A4 expression and utilizing the score as a companion or complementary diagnostic or to treat or ameliorate cancer or a tumor.
G01N 33/574 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet pour le cancer
G01N 33/577 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet faisant intervenir des anticorps monoclonaux
Extended duration cell analysis is provided via a device, comprising: a chamber configured to accept: a sample carrier, that includes a plurality of wells having electrodes in electrical communication with an impedance meter; and a cartridge, including a compound and at least one delivery port for the compound; a camera configured to capture images of a cell culture in each well via associated windows in the wells when the sample carrier is positioned at a first location; a fluid handler, in communication with the sample carrier and the cartridge when the sample carrier is positioned at a second location, configured to deliver the compound from the cartridge to a given well based on an impedance measurement of a sample in the given well; and a motion stage configured to move the sample carrier between the first location and the second location.
G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01J 19/00 - Procédés chimiques, physiques ou physico-chimiques en généralAppareils appropriés
G01N 33/50 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique
G05B 19/04 - Commande à programme autre que la commande numérique, c.-à-d. dans des automatismes à séquence ou dans des automates à logique
Systems and methods are disclosed for capturing and interpreting data streams between an instrument and a controlling device. A processor is configured to receive a data stream sent by the instrument to the controlling device and identify data frames in the stream. The processor is configured to search for a bit pattern in the stream, identify bits corresponding to a message length of a first presumed data frame based on a location relative to the bit pattern, and identify a second presumed data frame in the data stream based on the message length of the first presumed data frame. The processor is further configured to identify a second instance of the bit pattern, increase a count, continue scanning, extract and store instrument measurement data or operational metadata from the identified data frames, and analyze or interpret the captured data and metadata for visualization or alerts.
H04Q 9/00 - Dispositions dans les systèmes de commande à distance ou de télémétrie pour appeler sélectivement une sous-station à partir d'une station principale, sous-station dans laquelle un appareil recherché est choisi pour appliquer un signal de commande ou pour obtenir des valeurs mesurées
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
Extended duration cell analysis is provided via a device, comprising: a chamber configured to accept: a sample carrier, that includes a plurality of wells having electrodes in electrical communication with an impedance meter; and a cartridge, including a compound and at least one delivery port for the compound; a camera configured to capture images of a cell culture in each well via associated windows in the wells when the sample carrier is positioned at a first location; a fluid handler, in communication with the sample carrier and the cartridge when the sample carrier is positioned at a second location, configured to deliver the compound from the cartridge to a given well based on an impedance measurement of a sample in the given well; and a motion stage configured to move the sample carrier between the first location and the second location.
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
G01N 27/07 - Structure des récipients de mesureÉlectrodes pour ces récipients
71.
Substrate orientation device for liquid dispensing operations
A substrate orientation device includes a frame that supports a substrate on which liquid is dispensed, for example as may relate to (bio)chemical microarray fabrication. The frame is movable to different positions, such as for mounting the substrate to the frame, depositing droplets on the substrate, and applying a bulk liquid to the substrate. The substrate orientation device may be movable to different locations, such as different liquid dispensing devices, for example a printer and a flow cell.
A liquid dispensing device provides an input flow of liquid from a single inlet into an internal manifold, and distributes or divides the input flow from the manifold into a group of individual liquid circuits that direct respective output flows to outlets, from which controlled volumes of liquid can be dispensed into respective containers such as the wells of a multi-well plate. The volume of liquid dispensed into one container may be different from that dispensed into another container. Each liquid circuit may include a valve that regulates the liquid flow in that liquid circuit, which may be based on feedback from flow rate sensors. Two or more liquid dispensing devices may be stacked horizontally to provide additional unique liquid circuits and different liquids if desired.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
G01N 30/32 - Contrôle des paramètres physiques du fluide vecteur de la pression ou de la vitesse
41 - Éducation, divertissements, activités sportives et culturelles
42 - Services scientifiques, technologiques et industriels, recherche et conception
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An autoclaving microplate washing system for cells and non-adhering three-dimensional (3D) cell cultures includes one or more pumps for controlling the dispensing of washing fluid and the evacuation of fluid from microwells to gently wash the cells. A method of controlling the autoclaving microplate washing system includes controlling the one or more pumps for dispensing and evacuation.
F04B 43/12 - "Machines", pompes ou installations de pompage ayant des organes de travail flexibles à action péristaltique
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
B01L 9/00 - Dispositifs de supportDispositifs de serrage
B05B 1/14 - Buses, têtes de pulvérisation ou autres dispositifs de sortie, avec ou sans dispositifs auxiliaires tels que valves, moyens de chauffage avec des orifices de sortie multiplesBuses, têtes de pulvérisation ou autres dispositifs de sortie, avec ou sans dispositifs auxiliaires tels que valves, moyens de chauffage avec des filtres placés dans ou à l'extérieur de l'orifice de sortie
B08B 3/02 - Nettoyage par la force de jets ou de pulvérisations
B08B 11/02 - Dispositifs pour maintenir les objets pendant le nettoyage
F04B 13/00 - Pompes spécialement modifiées pour débiter des quantités fixes ou prédéterminées
F04B 23/06 - Combinaisons de plusieurs pompes les pompes étant toutes du type à déplacement positif alternatif
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
76.
LIQUID DISPENSING DEVICE FOR FILLING MULTIPLE CONTAINERS
A liquid dispensing device provides an input flow of liquid from a single inlet into an internal manifold, and distributes or divides the input flow from the manifold into a group of individual liquid circuits that direct respective output flows to outlets, from which controlled volumes of liquid can be dispensed into respective containers such as the wells of a multi-well plate. The volume of liquid dispensed into one container may be different from that dispensed into another container. Each liquid circuit may include a valve that regulates the liquid flow in that liquid circuit, which may be based on feedback from flow rate sensors. Two or more liquid dispensing devices may be stacked horizontally to provide additional unique liquid circuits and different liquids if desired.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
In some examples, linear and non-linear range-based plot pane selection may include receiving data that is to be displayed. Based on an increase or a decrease in a size of a range selector, a selection of a range of plot panes may be received from a plurality of available plot panes to display the data. Based on the received selection of the range of plot panes, a display of the data may be generated in plot panes included in the range of plot panes. The size of the range selector may be non-linearly proportional to the available plot panes to display the data.
G06F 3/0482 - Interaction avec des listes d’éléments sélectionnables, p. ex. des menus
G06F 3/04845 - Techniques d’interaction fondées sur les interfaces utilisateur graphiques [GUI] pour la commande de fonctions ou d’opérations spécifiques, p. ex. sélection ou transformation d’un objet, d’une image ou d’un élément de texte affiché, détermination d’une valeur de paramètre ou sélection d’une plage de valeurs pour la transformation d’images, p. ex. glissement, rotation, agrandissement ou changement de couleur
80.
CHROMATOGRAPHY COLUMN FOR THE SEPARATION OF SURFACTANTS
A chromatographic separation column for characterizing surfactant purity is disclosed. The column includes a separation medium having a particulate porous substrate with monofunctional silane with diisopropyl side chain groups and a pendant cyano functional group held within a column. The porous substrate has a pore size of about 50 Å to about 500 Å and an average particle size of about 1.0 μm to about 100 μm. The column has an inner diameter of about 1.0 to 100 mm, e.g., 4.6 mm and a length from about 10 mm to about 250 mm. Methods of facilitating characterization of polysorbate 80 by providing a sample containing polysorbate 80 and one or more reaction products of polysorbate 80 and a chromatographic separation column are disclosed. Methods of characterizing polysorbate 80 by separating polysorbate 80 and one or more reaction products of polysorbate 80 are also disclosed.
G01N 30/88 - Systèmes intégrés d'analyse, spécialement adaptés à cet effet, non couverts par un seul des groupes
B01D 15/10 - Adsorption sélective, p. ex. chromatographie caractérisée par des caractéristiques de structure ou de fonctionnement
B01J 20/28 - Compositions absorbantes ou adsorbantes solides ou compositions facilitant la filtrationAbsorbants ou adsorbants pour la chromatographieProcédés pour leur préparation, régénération ou réactivation caractérisées par leur forme ou leurs propriétés physiques
B01J 20/286 - Phases reliées chimiquement à un substrat, p. ex. à de la silice ou à des polymères
B01J 20/30 - Procédés de préparation, de régénération ou de réactivation
In some examples, a multi-device removal and installation tool may include a device removal tool including a first side having a first receiver with a first dimension that removably receives a first device, and a second side having a second receiver with a second dimension that removably receives a second device. The first dimension may be different from the second dimension. Further, the first receiver may include a plurality of separators to separate a surface of the first receiver from a surface of the first device.
B25B 27/14 - Outils à main ou outillage d'établi, spécialement conçus pour assembler ou séparer des pièces ou des objets, que cela entraîne ou non une certaine déformation, non prévus ailleurs pour assembler des objets autrement que par ajustage à la presse, ou pour les détacher
C07K 16/28 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains contre des récepteurs, des antigènes de surface cellulaire ou des déterminants de surface cellulaire
C07K 16/18 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux contre du matériel provenant d'animaux ou d'humains
C07K 16/00 - Immunoglobulines, p. ex. anticorps monoclonaux ou polyclonaux
G01N 33/53 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet
G01N 33/531 - Production de matériaux de tests immunochimiques
The present invention discloses a gas chromatography (GC) system for separating analytes from a matrix, comprising: a GC system entrance; a first column fluidically connected to the GC system entrance through a first valve; a second column fluidically connected to the first column through a second valve; a third column fluidically connected to the first column through the second valve; a fourth column fluidically connected to a third column through a third valve; and a GC system exit fluidically connected to both the fourth column and the second column through a fourth valve. And the present invention also discloses a method of separating various analytes, including analyte portions comprising one or more of Ar, O2, N2, CH4 and CO2, from a gas matrix and from CO2 by using the GC system.
G01N 30/46 - Modèles d'écoulement utilisant plus d'une colonne
B01D 53/02 - Séparation de gaz ou de vapeursRécupération de vapeurs de solvants volatils dans les gazÉpuration chimique ou biologique des gaz résiduaires, p. ex. gaz d'échappement des moteurs à combustion, fumées, vapeurs, gaz de combustion ou aérosols par adsorption, p. ex. chromatographie préparatoire en phase gazeuse
84.
FLUIDICALLY COUPLING OF SAMPLING AND SEPARATION PATHS
A switching unit is configured for selectively fluidically coupling a sampling volume, a sampling drive, a mobile phase drive, and a separating device. In a sample load configuration, the switching unit is configured for fluidically coupling the sampling volume and the sampling drive, for moving the fluidic sample into the sampling volume. In a decouple configuration, the switching unit is configured for fluidically coupling the sampling volume between the sampling drive and the separating device, while the mobile phase drive is fluidically decoupled from the separating device. In a sample introduction configuration, the switching unit is configured for fluidically coupling the mobile phase drive, the sampling volume, and the separating device for introducing at least an amount of the fluidic sample stored in the sampling volume into the mobile phase for fluid separation by the separating device.
A process of operating an analytical device (100) for analyzing a sample, the process comprising monitoring a present user behavior when operating the analytical device (100), comparing the monitored present user behavior with a prior user behavior, and when determining a discrepancy between the monitored present user behavior and the prior user behavior, triggering an action.
G01N 30/88 - Systèmes intégrés d'analyse, spécialement adaptés à cet effet, non couverts par un seul des groupes
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
Methods and systems for synthesizing an oligonucleotide in a reaction chamber comprising a solid support. A nucleotide monomer is selected for addition to an oligonucleotide. An inlet stream comprising the selected monomer is fed to the reaction chamber (column) of the reaction chamber and conditions are provided in the reaction chamber for oligonucleotide synthesis. An outlet stream from the reaction chamber can be split and directed to the waste drain and/or to an analysis station wherethe eluent is analyzed by mass spectrometry (MS) to confirm the identity of the monomer fed through the inlet to the reaction chamber. Based on results from analyzing by MS, it is determined whether the outlet stream comprises the selected nucleotide monomer or a derivative thereof.
C12Q 1/6809 - Méthodes de détermination ou d’identification des acides nucléiques faisant intervenir la détection différentielle
C07H 1/00 - Procédés de préparation des dérivés du sucre
C07H 21/00 - Composés contenant au moins deux unités mononucléotide comportant chacune des groupes phosphate ou polyphosphate distincts liés aux radicaux saccharide des groupes nucléoside, p. ex. acides nucléiques
A restriction in a liquid network containing a liquid is determined by generating a vacuum bubble within the liquid network, the vacuum bubble representing a volume in which the liquid has been substantially removed. A period of time between generating the vacuum bubble and until the volume has been substantially filled with the liquid is determined. A conclusion regarding the restriction is made based on the determined period of time.
G01M 3/26 - Examen de l'étanchéité des structures ou ouvrages vis-à-vis d'un fluide par utilisation d'un fluide ou en faisant le vide par mesure du taux de perte ou de gain d'un fluide, p. ex. avec des dispositifs réagissant à la pression, avec des indicateurs de débit
G01N 30/32 - Contrôle des paramètres physiques du fluide vecteur de la pression ou de la vitesse
In some examples, a vessel mounting system may include a first collar including at least one protrusion engageable with a groove of a vessel to align the first collar relative to the vessel. A second collar may include at least one stud disposable in a corresponding aperture of the first collar to align the second collar relative to the first collar.
In some examples, a vessel mounting system may include a first collar including at least one flexible member engageable with a groove of a vessel to align the first collar relative to the vessel. A second collar may include at least one clip engageable with a complementary clip of the first collar to engage the second collar with the first collar.
Methods and systems for synthesizing an oligonucleotide in a reaction chamber comprising a solid support. A nucleotide monomer is selected for addition to an oligonucleotide. An inlet stream comprising the selected monomer is fed to the reaction chamber (column) of the reaction chamber and conditions are provided in the reaction chamber for oligonucleotide synthesis. An outlet stream from the reaction chamber can be split and directed to the waste drain and/or to an analysis station where the eluent is analyzed by mass spectrometry (MS) to confirm the identity of the monomer fed through the inlet to the reaction chamber. Based on results from analyzing by MS, it is determined whether the outlet stream comprises the selected nucleotide monomer or a derivative thereof.
In some examples, a vessel mounting system may include a first collar and a second collar. The second collar may include at least one positioning member to position the second collar relative to the first collar. Further, the second collar may include at least one interlocking member to lock the second collar to the first collar.
In some examples, a vessel mounting system may include a first collar and a second collar. The second collar may include a clip engageable with an edge of the first collar to engage the second collar with the first collar.
Liquids are dispensed onto a substrate in a manner that reduces droplet evaporation while and after the liquids are dispensed. The substrate is at least partially enclosed by boundaries that form a chamber that is effective to reduce the rate of evaporation of droplets being dispensed. The chamber may be partially open to a surrounding environment. Additional evaporative liquid may be provided to assist in reducing the rate of evaporation. The liquid dispensing may be implemented, for example, as part of the fabrication of a microarray such as a chemical or biochemical array, which may be the product of DNA or RNA array printing.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
Biopolymers are synthesized on a substrate in a manner that that reduces droplet evaporation including at elevated temperatures. The substrate is enclosed by a lid that form an incubation chamber that is effective to impair evaporation of droplets being dispensed and to provide vapor and temperature adjustments. The biopolymer synthesis may be implemented, for example, as part of the fabrication of a microarray which may be the product of DNA or RNA array printing and for oligonucleotide library synthesis, by chemical and enzymatic procedures.
Provided herein are compositions and methods for inducing CRISPR/Cas-based editing of a target nucleic acid (e.g., target DNA or target RNA) in vitro or in a cell, using modified prime editing guide RNAs (pegRNAs) that incorporate one or more chemically-modified nucleotides. The modified pegRNAs disclosed herein may be used to induce Cas-mediated incorporation of one or more nucleotide changes and/or targeted mutagenesis of a target nucleic acid. The nucleotide change can include, e.g., one or more nucleotide changes, an insertion of one or more nucleotides, or a deletion of one or more nucleotides.
The invention provides methods and compositions for hybridizing at least one molecule to a target. The composition comprises at least one nucleic acid sequence, formamide, and a hybridization solution, wherein the concentration of formamide is less than or equal to 25%.
A system for monitoring cells, which includes a device for monitoring cell-substrate impedance, the device having a plurality of wells on a nonconductive substrate, where each of the plurality of wells has an electrode array fabricated on the substrate for measurement of cell-substrate impedance; an impedance analyzer that measures cell-substrate impedance from the plurality of wells; electronic circuitry with multiple analogue-to-digital conversion channels, where the electronic circuitry electrically connects the electrode arrays to the impedance analyzer such that the electrode arrays are electrically monitored at millisecond time resolution; and a software program that analyzes the measured cell-substrate impedance.
G01N 33/483 - Analyse physique de matériau biologique
G01N 27/02 - Recherche ou analyse des matériaux par l'emploi de moyens électriques, électrochimiques ou magnétiques en recherchant l'impédance
G01N 33/487 - Analyse physique de matériau biologique de matériau biologique liquide
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
In some examples, a multi-device removal and installation tool may include a device removal tool including catch members engageable with an engagement face of a device to attach, upon engagement of the catch members with the engagement face, the device removal tool to the device. The catch members may engage the engagement face with a removal force that is sufficient to remove, upon engagement of the catch members with the engagement face, the device from an instrument. The multi-device removal and installation tool may further include a torque limit tool including a retainer having at least one protrusion, and a drive member having an inside surface and an outside surface. The torque limit tool may further include at least one detent positioned on the inside surface.
H01J 49/06 - Dispositifs électronoptiques ou ionoptiques
B25B 27/14 - Outils à main ou outillage d'établi, spécialement conçus pour assembler ou séparer des pièces ou des objets, que cela entraîne ou non une certaine déformation, non prévus ailleurs pour assembler des objets autrement que par ajustage à la presse, ou pour les détacher
H01J 49/04 - Dispositions pour introduire ou extraire les échantillons devant être analysés, p. ex. fermetures étanches au videDispositions pour le réglage externe des composants électronoptiques ou ionoptiques
An enclosure for a fluid pump includes an outer shell defining an enclosure interior, a stator holder disposed in the enclosure interior and at least partially surrounding a motor stator of a motor, and conduits configured to direct air through the enclosure interior. The outer shell includes air inlets positioned to allow air to flow from an ambient space outside of the enclosure into the enclosure interior, and air outlets positioned to allow air to flow from the enclosure interior to the ambient space. The enclosure defines air flow paths running from at least some of the air inlets, to and through the air conduits, and from the air conduits to at least some of the air outlets, thereby cooling the motor during operation. Electronics may be positioned in the enclosure interior, which may also be cooled by the configuration of the enclosure.
The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, eliminate the use of, or reduce the dependence on formamide in hybridization. Compositions for use in the invention include an aqueous composition comprising at least one nucleic acid sequence and at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.
