Sample preparation methods for in situ RNA or DNA analysis, methods and compositions used in such methods are provided. Methods provided herein allow DNA or RNA preparation and downstream analysis to be carried out in the same tube or on an aliquot of the prepared sample without centrifugation or further purification. The compositions and methods provided herein can advantageously be used on a variety of samples, including cultures of cell lines and/or primary cells. The preparation process is amenable to high throughput processing using manual or robotic platforms.
A method for user guided initiating of an instrument includes receiving a run plan via a user interface of the instrument; indicating on the user interface, based on the run plan, a consumable to be provided to the instrument; detecting the presence of the consumable using a vision system; and indicating the presence of the consumable via the user interface.
G06T 1/00 - Traitement de données d'image, d'application générale
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
3.
LIPIDS FOR DELIVERY OF NUCLEIC ACIDS TO EUKARYOTIC CELLS
Ionizable lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
C07C 215/28 - Composés contenant des groupes amino et hydroxy liés au même squelette carboné ayant des groupes hydroxy et des groupes amino liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant non saturé et contenant des cycles aromatiques à six chaînons
C07C 217/42 - Composés contenant des groupes amino et hydroxy éthérifiés liés au même squelette carboné ayant des groupes hydroxy éthérifiés et des groupes amino liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant acyclique et saturé ayant des groupes hydroxy éthérifiés et au moins deux groupes amino liés au squelette carboné
C07C 219/06 - Composés contenant des groupes amino et hydroxy estérifiés liés au même squelette carboné ayant des groupes hydroxy estérifiés et des groupes amino liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant acyclique et saturé les groupes hydroxy étant estérifiés par des acides carboxyliques ayant les groupes carboxyle estérifiants liés à des atomes d'hydrogène ou à des atomes de carbone acycliques d'un squelette carboné acyclique saturé
C07C 219/08 - Composés contenant des groupes amino et hydroxy estérifiés liés au même squelette carboné ayant des groupes hydroxy estérifiés et des groupes amino liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant acyclique et saturé au moins un des groupes hydroxy étant estérifié par un acide carboxylique ayant le groupe carboxyle estérifiant lié à un atome de carbone acyclique d'un squelette carboné acyclique non saturé
C07C 229/12 - Composés contenant des groupes amino et carboxyle liés au même squelette carboné ayant des groupes amino et carboxyle liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant acyclique et saturé ayant un seul groupe amino et un seul groupe carboxyle liés au squelette carboné l'atome d'azote du groupe amino étant lié de plus à des atomes de carbone acycliques ou à des atomes de carbone de cycles autres que des cycles aromatiques à six chaînons à des atomes de carbone de squelettes carbonés acycliques
C07C 229/22 - Composés contenant des groupes amino et carboxyle liés au même squelette carboné ayant des groupes amino et carboxyle liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant acyclique et saturé le squelette carboné étant substitué de plus par des atomes d'oxygène
C07C 229/26 - Composés contenant des groupes amino et carboxyle liés au même squelette carboné ayant des groupes amino et carboxyle liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant acyclique et saturé ayant plus d'un groupe amino lié au squelette carboné, p. ex. lysine
C07C 229/36 - Composés contenant des groupes amino et carboxyle liés au même squelette carboné ayant des groupes amino et carboxyle liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné contenant des cycles aromatiques à six chaînons avec au moins un groupe amino et un groupe carboxyle liés au même atome de carbone du squelette carboné
C07C 323/25 - Thiols, sulfures, hydropolysulfures ou polysulfures substitués par des halogènes, des atomes d'oxygène ou d'azote ou par des atomes de soufre ne faisant pas partie de groupes thio contenant des groupes thio et des atomes d'azote, ne faisant pas partie de groupes nitro ou nitroso, liés au même squelette carboné ayant les atomes de soufre des groupes thio liés à des atomes de carbone acycliques du squelette carboné le squelette carboné étant acyclique et saturé
C07D 209/20 - Radicaux substitués par des atomes de carbone comportant trois liaisons à des hétéro-atomes, avec au plus une liaison à un halogène, p. ex. radicaux ester ou nitrile substitués en outre par des atomes d'azote, p. ex. tryptophane
C07D 233/61 - Composés hétérocycliques contenant des cycles diazole-1, 3 ou diazole-1, 3 hydrogéné, non condensés avec d'autres cycles comportant deux liaisons doubles entre chaînons cycliques ou entre chaînons cycliques et chaînons non cycliques avec uniquement des atomes d'hydrogène ou des radicaux ne contenant que des atomes d'hydrogène et de carbone, liés aux atomes de carbone du cycle avec des radicaux hydrocarbonés, substitués par des atomes d'azote ne faisant pas partie d'un radical nitro, liés aux atomes d'azote du cycle
C07C 215/14 - Composés contenant des groupes amino et hydroxy liés au même squelette carboné ayant des groupes hydroxy et des groupes amino liés à des atomes de carbone acycliques du même squelette carboné le squelette carboné étant saturé et acyclique l'atome d'azote du groupe amino étant de plus lié à des groupes hydrocarbonés substitués par des groupes amino
A61K 9/127 - Vecteurs à bicouches synthétiques, p. ex. liposomes ou liposomes comportant du cholestérol en tant qu’unique agent tensioactif non phosphatidylique
C07C 279/14 - Dérivés de la guanidine, c.-à-d. composés contenant le groupe les atomes d'azote liés par des liaisons simples ne faisant pas partie de groupes nitro ou nitroso ayant des atomes d'azote de groupes guanidine liés à des atomes de carbone acycliques d'un squelette carboné étant substitué de plus par des groupes carboxyle
Ionizable lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
The present invention provides mutant DNA polymerases, polynucleotides encoding the polymerases, cassettes and vectors including such polynucleotides, and cells containing the polymerases, polynucleotides, cassettes, and/or vectors of the invention. The present invention also provides methods for synthesizing polynucleotides and kits including a DNA polymerase of the invention.
A detection system that operates with reduced sample waste and dead volume, the system including: a module configured to introduce a sample spacer into a sample; at least one light source, wherein the light source illuminates the sample spacer and the sample, wherein illumination of the sample spacer produces scattered light; and a detection device configured to initiate acquisition of data related to the sample in response to scattered light detected by the detection device.
Examples described herein provide systems and methods for quantifying cells. An example method includes receiving at least one image, improving a contrast of the at least one image to generate a contrast image, and performing a fit operation on the contrast image to generate a processed image. The method includes applying a filter to the processed image to generate a filtered image, identifying cells within the filtered image, and providing an output image including an indication of the cells.
A detection system that operates with reduced sample waste and dead volume, the system including: a module configured to introduce a sample spacer into a sample; at least one light source, wherein the light source illuminates the sample spacer and the sample, wherein illumination of the sample spacer produces scattered light; and a detection device configured to initiate acquisition of data related to the sample in response to scattered light detected by the detection device.
A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.
Examples described herein provide systems and methods for quantifying cells. An example method includes receiving at least one image, improving a contrast of the at least one image to generate a contrast image, and performing a fit operation on the contrast image to generate a processed image. The method includes applying a filter to the processed image to generate a filtered image, identifying cells within the filtered image, and providing an output image including an indication of the cells.
G06T 7/136 - DécoupageDétection de bords impliquant un seuillage
G06V 10/50 - Extraction de caractéristiques d’images ou de vidéos en effectuant des opérations dans des blocs d’imagesExtraction de caractéristiques d’images ou de vidéos en utilisant des histogrammes, p. ex. l’histogramme de gradient orienté [HoG]Extraction de caractéristiques d’images ou de vidéos en utilisant l’addition des valeurs d’intensité d’imageAnalyse de projection
G06V 20/69 - Objets microscopiques, p. ex. cellules biologiques ou pièces cellulaires
16.
SYSTEMS AND METHODS FOR DETECTING STRUCTURAL VARIANTS
Systems and method for identifying gene fusions can obtain sequencing information for a plurality of amplicons from a nucleic acid sample. The sequencing information can include a plurality of reads that are initially partially mapped to a reference sequence. Fragments may be generated by splitting the partially mapped reads into mapped and unmapped fragments, and the fragments may be remapped to the reference sequence. Gene fusions can be identified based on reads where the first fragment maps to a first gene and the second fragment maps to a second gene.
Campylobacter spSalmonella spShigellasp./Enteroinvasive Escherichia coli (EIEC)Escherichia coli Escherichia coliEscherichia coli stx2/Shiga toxin B. Other embodiments include methods and kits for detecting diarrhea causing pathogens.
C12Q 1/689 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes pour les bactéries
A61K 39/40 - AnticorpsImmunoglobulinesImmunsérum, p. ex. sérum antilymphocitaire bactériens
18.
METHODS FOR GAS FILTRATION IN FLUID PROCESSING SYSTEMS
A method for filtering a gas comprises passing a gas through a compartment of a filter assembly, the filter assembly comprising: an inlet opening; a first outlet opening; a casing comprising polymeric film and bounding the compartment, the compartment communicating with the inlet opening and the first outlet opening; and a first filter at least partially disposed within the compartment. The method further comprising forming a first seal across a first section of the casing at a location between the inlet opening and the first filter to form a first sub-compartment within the casing and severing the casing at a first location.
B01D 46/58 - Filtres ou procédés spécialement modifiés pour la séparation de particules dispersées dans des gaz ou des vapeurs avec plusieurs éléments filtrants, caractérisés par leur disposition relative montés en parallèle
B01D 46/00 - Filtres ou procédés spécialement modifiés pour la séparation de particules dispersées dans des gaz ou des vapeurs
B01F 23/231 - Mélange de gaz avec des liquides en introduisant des gaz dans des milieux liquides, p. ex. pour produire des liquides aérés par barbotage
B01F 27/2121 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins caractérisés par leurs arbres de rotation composés de parties interconnectées
B01F 27/88 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins avec des agitateurs tournant autour d'un axe sensiblement vertical avec une unité récipient-agitateur séparée qui est adaptée pour être couplée à un mécanisme d'entraînement
B01F 27/90 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins avec des agitateurs tournant autour d'un axe sensiblement vertical avec des palettes ou des bras
An instrument for processing and/or measuring a biological process comprises a sample processing system and an excitation source exhibiting a spectral function of output power or intensity verses wavelength of output power or intensity. The spectral function has a minima wavelength corresponding to a local minima value of the output power or intensity; a first maxima wavelength corresponding to a first local maxima of output power or intensity, the output power or intensity at the first local maxima being greater than the output power or intensity at any wavelength less than the minima wavelength; a second maxima wavelength corresponding to a second local maxima of output power or intensity, the output power or intensity at the second local maxima being greater than the output at any wavelength greater than the minima wavelength; the minima wavelength is between the first maxima wavelength and the second maxima wavelength.
G01N 21/27 - CouleurPropriétés spectrales, c.-à-d. comparaison de l'effet du matériau sur la lumière pour plusieurs longueurs d'ondes ou plusieurs bandes de longueurs d'ondes différentes en utilisant la détection photo-électrique
20.
PURIFICATION CHEMISTRIES AND FORMATS FOR SANGER DNA SEQUENCING REACTIONS ON A MICRO-FLUIDICS DEVICE
According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.
The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.
C12Q 1/6876 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes
C12Q 1/6881 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour le typage de tissu ou de cellule, p. ex. sondes d’antigène leucocytaire humain [HLA]
C12Q 1/6883 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique
22.
COMPOSITIONS AND METHODS FOR REDUCING MASTER MIX CONTAMINATION
This disclosure describes master mix compositions, nucleic acid amplification kits, methods of manufacturing master mix compositions, and methods of using master mix compositions that minimize or eliminate the negative effects of contaminant nucleic acids. Conventional master mix compositions often include contaminant nucleic acids.
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
The charging stand kit for assembling a charging stand (1) for electrical pipettes (10) comprises a charging module (2) configured to hold an electrical pipette (10) to be charged, the charging module (2) comprising first and second sets of electrical contacts (7, 8) on first and second sides for connecting the charging module (2) electrically to adjacent parts (2, 3) of the charging stand (1), a first leg (3) having an upper end con- figured to be attached to the first side of the charging module (2) and comprising a set of electrical contacts (9) configured to contact the first set of electrical contacts (7) of the charging module (2), and a second leg (4) having an upper end config- ured to be attached to the second side of the charging module (2).
A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
A method for sequencing a nucleic acid template includes: (a) performing a first sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a first predetermined ordering of nucleotides and/or reagents to obtain a first sequencing result; (b) after the first sequencing process, performing a second sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a second predetermined ordering of nucleotides and/or reagents to obtain a second sequencing result, the second predetermined ordering of nucleotides and/or reagents being different from the first predetermined ordering of nucleotides and/or reagents and at least one of the first and second predetermined orderings of nucleotides and/or reagents being designed for repeat sequencing; and (c) determining a sequence of bases corresponding to at least a portion of the nucleic acid template using both the first sequencing result and the second sequencing result.
01 - Produits chimiques destinés à l'industrie, aux sciences ainsi qu'à l'agriculture
Produits et services
Chemicals for use in industry and science; diagnostic preparations for scientific or research use; diagnostic preparations for clinical or medical laboratory use; DNA polymerase, reagents and reagent kits comprising generic DNA circle, DNA polymerase and buffers for scientific, medical or veterinary research use; DNA polymerase, reagents and reagent kits comprising generic DNA circle, DNA primers, DNA polymerase and buffers for use in the biotechnology field
30.
LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR
Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C40B 20/04 - Identification des éléments d'une bibliothèque au moyen d'une étiquette, d'un marqueur ou d'un autre identificateur lisible ou détectable, p. ex. procédés de décodage
C40B 40/06 - Bibliothèques comprenant des nucléotides ou des polynucléotides ou leurs dérivés
C40B 50/06 - Procédés biochimiques, p. ex. utilisant des enzymes ou des micro-organismes viables entiers
31.
FLUID MIXING SYSTEMS WITH MODULAR IMPELLERS AND RELATED METHODS
A mixing system for mixing a liquid includes a first impeller segment having a first mount and a first mixing blade secured to the first mount and a second impeller segment having a second mount and a first mixing blade secured to the second mount, the second impeller segment being separate and discrete from the first impeller segment. One or more drive members are secured to the first impeller segment and the second impeller segment for concurrently rotating the first impeller segment and the second impeller segment about a rotational axis. The first impeller segment and the second impeller segment are secured to the one or more drive members so that a plane extending normal to the axis of rotation intersects with the first mixing blade of the first impeller segment and the first mixing blade of the second impeller segment.
B01F 27/191 - Agitateurs avec plusieurs éléments de mélange montés en séquence sur un même axe avec des éléments similaires
B01F 27/07 - Agitateurs caractérisés par leur montage sur l’arbre
B01F 27/072 - Agitateurs caractérisés par leur montage sur l’arbre caractérisés par la disposition des agitateurs par rapport à l'axe de rotation
B01F 27/113 - Agitateurs en forme d'hélice pour produire un écoulement axial, p. ex. en forme d'hélice de navire ou d'avion
B01F 27/91 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins avec des agitateurs tournant autour d'un axe sensiblement vertical avec des hélices
B01F 35/513 - Récipients souples, p. ex. sacs supportés par des conteneurs rigides
B01F 101/44 - Mélange d'ingrédients pour la microbiologie, l'enzymologie, la culture in vitro ou la manipulation génétique
C12M 1/06 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz avec agitateur, p. ex. avec agitateur à turbine
32.
SYSTEMS AND METHODS FOR IDENTIFYING SEQUENCE VARIATION
Systems and method for determining variants can receive mapped reads, align flow space information to a flow space representation of a corresponding portion of the reference. Reads spanning a position with a potential variant can be evaluated in a context specific manner. A list of probable variants can be provided.
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p. ex. séquençage par hybridation [SBH]
G16B 5/00 - TIC spécialement adaptées à la modélisation ou aux simulations dans la biologie des systèmes, p. ex. réseaux de régulation génétique, réseaux d’interaction entre protéines ou réseaux métaboliques
A method for reducing spectral crosstalk in a multiplexed assay is provided. The method includes receiving filter signal data from a multiplexed fluorescence assay and an initial dye matrix including calibrated spectral dye data. The method further includes generating an updated dye matrix based on the initial dye matrix and estimated crosstalk proxies, and then adjusting the updated dye matrix to meet a calculated optimization value. The calculated optimization value is calculated based on at least the estimated crosstalk proxies and the filter signal data. The calculated optimization value may be further calculated based on each adjustment of the updated dye matrix, and a cross-correlation between dyes used in the assay. The method further includes generating an improved dye matrix based on the adjusted updated dye matrix, and then generating spectral adjusted data based on the improved dye matrix.
C12Q 1/68 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des acides nucléiques
A system for sample holder scanning is provided. The system includes a light source, a multiband excitation filter configured to select at least two excitation bands of light for fluorescent dyes used in the sample holder. The system further includes a multiband dichroic filter configured to reflect the at least two excitation bands of light, a multiband emission filter configured to transmit at least two bands of fluorescent emission light from each reaction site of the sample holder. The multiband dichroic filter is further configured to transmit the at least two bands of fluorescent emission light. The system also includes an optical sensor configured to detect the at least two bands of fluorescent emission light to generate an image of a sample holder.
Assemblies, methods, and systems for delivering a fluid into a fluidic flow. An assembly includes: a dispenser body having an interior wall defining a lumen having a longitudinal axis and a diameter; a cap configured to traverse the diameter of the lumen, the cap having an upper surface and a lower surface; a piston disposed within the lumen, the piston having a top surface and a bottom surface, the top surface together with the interior wall and the lower surface of the cap defining a working volume of the lumen, wherein the piston is movable along the longitudinal axis to change the working volume; an outlet in fluid connection with the working volume; and a fluid conduit extending between a first portion of the fluid conduit and a second portion of the fluid conduit, the fluid conduit being in fluid connection with the working volume via the outlet between the first and second portions of the fluid conduit.
A61M 5/14 - Dispositifs de perfusion, p. ex. perfusion par gravitéPerfusion sanguineAccessoires à cet effet
A61J 1/14 - Récipients spécialement adaptés à des fins médicales ou pharmaceutiques DétailsAccessoires à cet effet
A61M 5/19 - Seringues avec plusieurs compartiments
A61M 5/24 - Seringues à ampoules, c.-à-d. seringues à aiguille utilisables avec des ampoules ou des cartouches échangeables, p. ex. automatiques
A61M 5/20 - Seringues automatiques, p. ex. avec tige de piston actionnée automatiquement, avec injection automatique de l'aiguille, à remplissage automatique
A61M 39/00 - Tubes, raccords ou accouplements pour tubes, soupapes, voies d'accès ou similaires, spécialement adaptés pour un usage médical
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
A61J 1/05 - Récipients spécialement adaptés à des fins médicales ou pharmaceutiques pour recueillir, stocker ou administrer du sang, du plasma ou des liquides à usage médical
A61M 5/168 - Moyens pour commander l'écoulement des agents vers le corps ou pour doser les agents à introduire dans le corps, p. ex. compteurs de goutte-à-goutte
A61M 5/145 - Perfusion sous pression, p. ex. utilisant des pompes utilisant des réservoirs sous pression, p. ex. au moyen de pistons
A61M 39/20 - Couvercles ou bouchons d'obturation pour connecteurs ou extrémités ouvertes de tubes
36.
SYSTEMS AND METHODS OF CELL SORTING IMPLEMENTING ARTIFICIAL INTELLIGENCE
Disclosed herein are apparatuses, systems, as well as related methods, computing devices, and computer-readable media related to real time cell sorter cell sorting using embeddings. For example, in some embodiments a method may comprise receiving first cell sorter data. The first cell sorter data may include cell sorter data including microscopy data, hyperspectral imaging data, high-dimensional vector data, or one or more combinations thereof. In some embodiments, the cell sorter data may include quantitative fluorescence data expressed as one or more of antibodies bound per cell, antibody binding capacity (ABC), molecules of equivalent soluble fluorochrome (MESF), one or more other quantitative indicators of fluorescence, or one or more combinations thereof. In some embodiments, the quantitative fluorescence data includes one or more fluorescence signals from: one or more fluorescent proteins, one or more fluorescent dyes, one or more fluorescently conjugate antibodies, or one or more combinations thereof.
G06V 10/40 - Extraction de caractéristiques d’images ou de vidéos
G06V 10/82 - Dispositions pour la reconnaissance ou la compréhension d’images ou de vidéos utilisant la reconnaissance de formes ou l’apprentissage automatique utilisant les réseaux neuronaux
G06V 20/69 - Objets microscopiques, p. ex. cellules biologiques ou pièces cellulaires
Disclosed herein are dual-expression polynucleotide vectors including a first polynucleotide sequence including, in the 5' to 3' direction, a bacteriophage promoter sequence operatively linked to a sequence encoding a plurality of first RNA hairpin structures, a multiple cloning site sequence, a sequence encoding a plurality of second RNA hairpin structures, and a transcription terminator sequence; and a second polynucleotide sequence including, in the 5' to 3' direction, a bacteriophage promoter sequence operatively linked to a ribosome binding site sequence, a viral coat protein sequence, and the transcription terminator sequence. RNA detection and/or quantification standards or controls produced from these dual-expression vectors, along with methods for producing the RNA detection and/or quantification standards or controls, and methods of detecting the presence or quantity of nucleic acid using the RNA detection and/or quantification standards, are also disclosed.
The present disclosure is directed to compositions, methods and kits useful for the synthesis of nucleic acid molecules. More specifically, compositions, methods and kits are provided for the amplification of nucleic acid molecules in an one-step qPCR or RT-qPCR procedure comprising a reverse transcriptase, a DNA polymerase, at least one dNTP, and at least one stabilizing agent, wherein said at least one stabilizing agent increases stability of an assembled polymerase chain reaction.
C12Q 1/6848 - Réactions d’amplification d’acides nucléiques caracterisées par les moyens d’empêcher la contamination ou d’augmenter la spécificité ou la sensibilité d’une réaction d’amplification
Disclosed are compositions, kits, and methods for amplifying and quantifying a target nucleic acid from a sample. Compositions, kits, and methods enable the quantification of a target nucleic acid from a sample using an internal quantification standard disposed in the same reaction volume as the target nucleic acid, thereby eliminating the need for additional amplification reactions and processing to generate a standard curve. Compositions, kits, and methods also enable the comparison of target nucleic acid loads between two or more test samples by normalizing measured levels of the target nucleic acid in each sample according to relative levels of endogenous nucleic acid in each test sample.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
40.
METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS
Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.
G01N 27/414 - Transistors à effet de champ sensibles aux ions ou chimiques, c.-à-d. ISFETS ou CHEMFETS
C12Q 1/6818 - Tests d’hybridation caractérisés par les moyens de détection impliquant l’interaction de plusieurs marqueurs, p. ex. transfert d’énergie de résonance
C12Q 1/6874 - Méthodes de séquençage faisant intervenir des réseaux d’acides nucléiques, p. ex. séquençage par hybridation [SBH]
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
H01L 27/088 - Dispositifs consistant en une pluralité de composants semi-conducteurs ou d'autres composants à l'état solide formés dans ou sur un substrat commun comprenant des éléments de circuit passif intégrés avec au moins une barrière de potentiel ou une barrière de surface le substrat étant un corps semi-conducteur comprenant uniquement des composants semi-conducteurs d'un seul type comprenant uniquement des composants à effet de champ les composants étant des transistors à effet de champ à porte isolée
H01L 29/423 - Electrodes caractérisées par leur forme, leurs dimensions relatives ou leur disposition relative ne transportant pas le courant à redresser, à amplifier ou à commuter
H01L 29/78 - Transistors à effet de champ l'effet de champ étant produit par une porte isolée
41.
METHODS FOR CONTEXT BASED COMPRESSION OF GENOMIC DATA FOR IMMUNO-ONCOLOGY BIOMARKERS
The method includes compressing numbers of reads data for targeted genes of a gene expression assay performed on a test sample. The targeted genes are organized into categories. Each category represents a functional context associated with the targeted genes in that category. The numbers of reads corresponding to targeted genes each category is compressed to form a compressed value for the category. The compressed value is compared to a baseline value for the category to determine an enrichment or a loss of a signature corresponding to the functional context of the category. The method may include analyzing information from multiple assays performed on the test sample, assigning a score value to each assay result and predicting a response to immune-oncology treatment based on the assigned scores.
Methods, systems, and devices for analyzing a sample for the presence of a target protein are provided. A sample can be processed, the processing generating an amplification product of a template nucleic acid formed in response to a target protein being present in the sample. The amplification product can be contacted with a detection probe to generate a second product comprising a labeled nucleic acid. A lateral flow substrate can be contacted with the second product, the lateral flow substrate comprising a capture moiety configured to bind the labeled nucleic acid. The methods can be carried out in one or more chambers of a single device or multiple devices. Detection probe bound to amplicons of the template nucleic acid captured on the lateral flow substrate can be read visually or using a sensor.
Life Technologies Holdings PTE Limited (Singapour)
Life Technologies Corporation (USA)
Inventeur(s)
Liaw, Wui Khen Kevin
Boo, Kuan Moon
Yeo, Huei Steven
Foo, Wern Yuh
Mead, Joshua Mathew
Bong, Justina Linkai
Makinen, Mikko
Ling, Mio Xiu Lu
Lee, Way Xuang
Chan, Wen Lun
Liu, Yunxiang
Abrégé
Multichannel pipettes, electroporation systems utilizing the multichannel pipettes and methods for electroporating a cell. The electroporation system includes a multichannel pipette, a pipette tip(s), a pipette docking assembly, and a pulse generator. The pipette docking assembly includes a pipette station, a pipette station guard, and a reservoir.
A rocking assembly for mixing and cooling a liquid, such as a cell containing liquid (e.g., cell-based therapeutic product), comprising a base unit (110) having a mount (120), a platform (130), a cooler module (150) operably connected to the platform and the mount, the cooler module configured to cool the platform; and an actuator in operable connection with the cooler module, the actuator being configured to move the platform in a rocking motion relative to the base unit. A cell processing system for fill and finish processes in the manufacture of cell-based therapeutics including the rocking assembly and a liquid dispensing system. A method for processing a liquid for fill and finish workflow applications.
An optical fiber apparatus and method for uniformly illuminating biological samples distributed across one or more arrays of microchambers of a microplate can establish more reliable Polymerase Chain Reaction (PCR) data sampling. In some embodiments, the apparatus comprises a light engine that emits a beam of light, which is captured by a fiber bundle for delivering one or more beams of light to each microchamber; whereby, each beam of light causes the biological sample to fluoresce. The fiber cables of the fiber bundle include distal output ends that are held in place using a mechanical stand, which serves to flexibly align the output beams of the distal output ends to be arranged such that the centers of the output light beams are directed to different locations in the one or more arrays of microchambers, where each of the adjacent ones of the output light beams partially overlap for uniform distribution of light.
Multichannel pipettes, electroporation systems utilizing the multichannel pipettes and methods for electroporating a cell. The electroporation system includes a multichannel pipette, a pipette tip(s), a pipette docking assembly, and a pulse generator. The pipette docking assembly includes a pipette station, a pipette station guard, and a reservoir.
The present disclosure provides systems and methods of electroporation protocol optimization. Embodiments include electroporation machines capable of carrying out test protocols including multiple user-designated parameters. The protocols and parameters can be carried out on samples comprising cells, including portions of a sample, to determine optimum parameters for electroporation for different samples. The systems and methods of optimization preferably use electroporation cartridges, electroporation instruments and systems and methods of electroporation using these devices and systems. In some embodiments, electroporation cartridges comprise an electroporation chamber and electrodes.
C12M 1/42 - Appareils pour le traitement de micro-organismes ou d'enzymes au moyen d'énergie électrique ou ondulatoire, p. ex. magnétisme, ondes sonores
C12M 1/36 - Appareillage pour l'enzymologie ou la microbiologie comportant une commande sensible au temps ou aux conditions du milieu, p. ex. fermenteurs commandés automatiquement
51.
DEVICES AND METHODS FOR LASER CAPTURE MICRODISSECTION
A carrier for holding a biological sample includes a substrate. The substrate is configured to engage a first sample chamber comprising a first opening characterized by a first opening diameter or a second sample chamber comprising a second opening characterized by a second opening diameter that is greater than the first opening diameter. The substrate includes an upper portion, a lower portion, and an intermediate portion disposed between the upper portion and the lower portion. The lower portion is disposed below the upper portion and comprises a bottom surface configured to receive a biological sample. The intermediate portion is characterized by a first substrate diameter and the lower portion is characterized by a second substrate diameter that is less than the first substrate diameter.
Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of SARS-CoV-2, including SARS-CoV-2 variants, or other coronaviruses from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
53.
Adjustable Foam Sensor Systems And Related Methods
An adjustable foam sensor system is disclosed. The adjustable foam sensor system can include an adjustable foam sensor coupled to a transition member with a foam probe extending from the transition member. The adjustable foam sensor system can also include an adjustable housing that can be coupled to a container interface for interfacing with a container of a fluid processing system. The adjustable housing can be configured to be compressed or extended in response to a force, which in turn can reposition or move the adjustable foam sensor in the container. A controller can automatically adjust the adjustable foam sensor based on sensed and/or determined fluid levels, as well as control the delivery of anti-foam for reducing foam in the container.
Provided herein are compositions, methods and uses that relate to or result from providing separation media having at least one flocculant ligand covalently attached to a base surface or support, and the separation and/or purification of biological molecules using the separation media of the present disclosure. Certain embodiments provide separation media which under certain modes of operation, enhance the separation of the molecule of interest from impurities.
B01J 39/05 - Procédés utilisant des échangeurs organiques sous forme fortement acide
B01D 15/36 - Adsorption sélective, p. ex. chromatographie caractérisée par le mécanisme de séparation impliquant une interaction ionique, p. ex. échange d'ions, paire d'ions, suppression d'ions ou exclusion d'ions
B01J 39/20 - Composés macromoléculaires obtenus par des réactions ne faisant intervenir que des liaisons carbone-carbone non saturées
B01J 47/014 - Procédés d'échange d'ions en généralAppareillage à cet effet dans lesquels les propriétés d’adsorption de l’échangeur d’ions sont utilisées, p. ex. récupération de protéines ou de composés macromoléculaires
Systems and methods of providing run-time quality control and monitoring of a single or multiple sequencing runs are provided herein. In some embodiments, the run-time system includes or is in communication with a processor capable of determining various types of run-time information relating to the quality, progress, etc. of various sequencing runs. In some embodiments, the system can also be in communication with a user interface, for example, a GUI, capable of representing and communicating various types of information to a user regarding the quality of the individual or multiple runs, the functioning of the instrument, an error event, etc. Additionally, the system can capable of receiving actionable information from a user via the GUI thereby allowing the user to terminate or repeat various sequencing steps in a particular run, terminate a entire run, terminate all runs, allow a run to proceed, etc.
B24B 41/06 - Supports de pièces, p. ex. lunettes réglables
B24B 5/04 - Machines ou dispositifs pour meuler des surfaces de révolution des pièces, y compris ceux qui meulent également des surfaces planes adjacentesAccessoires à cet effet possédant des pointes ou des mandrins pour maintenir la pièce pour meuler extérieurement des surfaces cylindriques
Methods and systems for detecting a sample via optical pathways are described herein. In one aspect, a light detection system can include: a first optical pathway configured to direct emissions from an interrogation site to a first detector; a second optical pathway configured to direct emissions from the interrogation site to a second detector; and an automated switching module configured to receive a signal that induces the automated switching module to switch between (i) a first state that directs emissions from the interrogation site to the first optical pathway or a second state that directs emissions from the interrogation site to the second optical pathway and (ii) the other of the first state and the second state.
The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, methods provide for determining convergence of T cell receptor and/or B cell receptor repertoires in samples prior to a treatment and predicting a subject's response to the treatment based on the measured convergence frequency. In another aspect, methods provide for an immune receptor haplotype group and predicting a subject's potential or predisposition to be protected from or vulnerable to an adverse event following a treatment.
C12Q 1/6886 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour les maladies provoquées par des altérations du matériel génétique pour le cancer
C40B 30/04 - Procédés de criblage des bibliothèques en mesurant l'aptitude spécifique à se lier à une molécule cible, p. ex. liaison anticorps-antigène, liaison récepteur-ligand
Methods are provided for making bispecific antibodies and antibody conjugates comprising site-specifically cross-linking two or more antibodies, antibody fragments or Fc-fusion proteins. Also provided are compositions and uses for the bispecific antibodies and antibody conjugates. The bispecific antibodies may be used to treat a disease or condition. Also provided are methods for site-specifically conjugating a liposome, an mRNA or an siRNA to an antibody, and uses of the antibody-conjugated liposome, mRNA or siRNA.
A61K 9/00 - Préparations médicinales caractérisées par un aspect particulier
A61K 47/60 - Préparations médicinales caractérisées par les ingrédients non actifs utilisés, p. ex. les supports ou les additifs inertesAgents de ciblage ou de modification chimiquement liés à l’ingrédient actif l’ingrédient non actif étant chimiquement lié à l’ingrédient actif, p. ex. conjugués polymère-médicament l’ingrédient non actif étant un agent de modification l’agent de modification étant un composé organique macromoléculaire, p. ex. une molécule oligomérique, polymérique ou dendrimérique obtenu par des réactions autres que celles faisant intervenir uniquement des liaisons non saturées carbone-carbone, p. ex. polyurées ou polyuréthanes le composé organique macromoléculaire étant un oligomère, un polymère ou un dendrimère de polyoxyalkylène, p. ex. PEG, PPG, PEO ou polyglycérol
A61K 47/68 - Préparations médicinales caractérisées par les ingrédients non actifs utilisés, p. ex. les supports ou les additifs inertesAgents de ciblage ou de modification chimiquement liés à l’ingrédient actif l’ingrédient non actif étant chimiquement lié à l’ingrédient actif, p. ex. conjugués polymère-médicament l’ingrédient non actif étant un agent de modification l’agent de modification étant un anticorps, une immunoglobuline ou son fragment, p. ex. un fragment Fc
A61K 47/69 - Préparations médicinales caractérisées par les ingrédients non actifs utilisés, p. ex. les supports ou les additifs inertesAgents de ciblage ou de modification chimiquement liés à l’ingrédient actif l’ingrédient non actif étant chimiquement lié à l’ingrédient actif, p. ex. conjugués polymère-médicament le conjugué étant caractérisé par sa forme physique ou sa forme galénique, p. ex. émulsion, particule, complexe d’inclusion, stent ou kit
C12N 9/24 - Hydrolases (3.) agissant sur les composés glycosyliques (3.2)
C12N 15/113 - Acides nucléiques non codants modulant l'expression des gènes, p. ex. oligonucléotides anti-sens
C12P 21/00 - Préparation de peptides ou de protéines
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
59.
SYSTEMS AND METHODS FOR PROVIDING FLUIDIC ACCESS TO A FLOW CELL
A method of applying a fluid composition to a flow cell of a sensor device includes inserting the sensor device into a holder; pipetting an aliquot of the fluid composition into a pipette tip; and applying the aliquot of the fluid composition to an opening of a fluidic connector engaged with the flow cell of the sensor device. The fluidic connector includes a body; and a plurality of fluidics interfaces formed in the body, each fluidic interface of the plurality of fluidics interfaces includes an opening, a first port in fluid communication with the opening, a second port, and a third port in fluidic communication with the second port.
A bioprocessing control system can include an intelligent gateway. The gateway can receive, from a controller, a request for data associated with the plurality of bioprocessing instruments including a primary data set and a secondary data set. The primary data set includes a block of primary data measured by the plurality of bioprocessing instruments. The secondary data set has a lower priority than the primary data set. The gateway can cause the controller to control the bioprocessing instrument by at least: sending, to the controller in response to the request, the block of primary data. The gateway can send, to the controller during the sending the block of primary data and in response to the request, a portion of the secondary data set, thereby reducing a load on the controller.
G05B 13/02 - Systèmes de commande adaptatifs, c.-à-d. systèmes se réglant eux-mêmes automatiquement pour obtenir un rendement optimal suivant un critère prédéterminé électriques
H04L 12/66 - Dispositions pour la connexion entre des réseaux ayant différents types de systèmes de commutation, p. ex. passerelles
61.
EXTRACELLULAR VESICLE PROTEIN AND NUCLEIC ACID ANALYSIS
Methods of detecting one or more protein and one or more nucleic acid targets in EVs are provided. The methods include capturing EVs from a sample on a solid surface or bead to produce captured EVs, labeling the captured EVs with one or more detectably labeled antibodies, contacting the captured EVs with a fixation reagent, contacting the captured EVs with a permeabilization reagent, contacting the captured EVs with one or more nucleic acid detection reagents comprising a signal generating system, and detecting the one or more labeled antibodies and detecting a signal generated by the signal generating system, thereby detecting the one or more protein and one or more nucleic acid targets in the EVs.
G01N 33/543 - Tests immunologiquesTests faisant intervenir la formation de liaisons biospécifiquesMatériaux à cet effet avec un support insoluble pour l'immobilisation de composés immunochimiques
C12N 15/10 - Procédés pour l'isolement, la préparation ou la purification d'ADN ou d'ARN
C12Q 1/6804 - Analyse d’acides nucléiques utilisant des immunogènes
G01N 33/58 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des substances marquées
G01N 33/68 - Analyse chimique de matériau biologique, p. ex. de sang ou d'urineTest par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligandsTest immunologique faisant intervenir des protéines, peptides ou amino-acides
The present invention relates to automated separation systems used for purifying multiphase fluid generated from bioreactors to prevent unintentional clogging of gas exhaust filters. In an embodiment, the separation system includes a separator assembly configured to generate a vortex motion for the multiphase fluid to efficiently break foam, release particle matter entrapped in the foam and release gases free of particle matter for passing through gas exhaust filters.
Systems and methods that enable analyte detection in a multiplexed amplification process can include obtaining, at multiple time points during the amplification process, composite fluorescence signal data associated with a composite fluorescence signal from at least a first probe type comprising a first fluorophore and a second probe type comprising a second fluorophore which has substantially overlapping spectral characteristics as said first fluorophore, the first probe type and the second probe type differing in thermal and/or temporal properties; and determining, based at least partially on the composite fluorescence signal data, fluorescence signal data associated with a fluorescence signal from a given probe type of the first probe type or the second probe type during the amplification process.
C12Q 1/6816 - Tests d’hybridation caractérisés par les moyens de détection
C12Q 1/6818 - Tests d’hybridation caractérisés par les moyens de détection impliquant l’interaction de plusieurs marqueurs, p. ex. transfert d’énergie de résonance
The instant technology generally relates to methods and compositions for expansion of mesenchymal stem cells in culture on a modified surface in the absence of a cell feeder layer and the absence of a cell adhesive coating on the surface. In some instances, the mesenchymal stem cells are expanded on the modified surface in a serum-free culture medium.
A61K 35/28 - Moelle osseuseCellules souches hématopoïétiquesCellules souches mésenchymateuses de toutes origines, p. ex. cellules souches dérivées de tissu adipeux
A fluid mixing system includes a collapsible container bounding a compartment and extending between a first end and an opposing second end. An elongated continuous drive line is at least partially disposed within the compartment of the container. A first portion of the drive line is laterally spaced apart from a second portion of the drive line, and the first portion of the drive line and the second portion of the drive line are rotatable within the compartment of the container.
C12M 1/06 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz avec agitateur, p. ex. avec agitateur à turbine
B01F 27/1111 - Agitateurs centrifuges, c.-à-d. agitateurs à sortie radialeAgitateurs de type turbine, p. ex. avec des moyens pour guider le flux avec un disque plat ou avec un élément en forme de disque équipé de pales, p. ex. une turbine de Rushton
B01F 27/191 - Agitateurs avec plusieurs éléments de mélange montés en séquence sur un même axe avec des éléments similaires
B01F 35/41 - Montage ou support des arbres d'agitation ou des unités d'agitation sur les récipients
B01F 35/513 - Récipients souples, p. ex. sacs supportés par des conteneurs rigides
B01F 101/44 - Mélange d'ingrédients pour la microbiologie, l'enzymologie, la culture in vitro ou la manipulation génétique
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/02 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'agitationAppareillage pour l'enzymologie ou la microbiologie avec des moyens d'échange de chaleur
C12N 1/00 - Micro-organismes, p. ex. protozoairesCompositions les contenantProcédés de culture ou de conservation de micro-organismes, ou de compositions les contenantProcédés de préparation ou d'isolement d'une composition contenant un micro-organismeLeurs milieux de culture
Disclosed are master mix compositions, kits, and related methods for use in amplifying a target nucleic acid. A master mix composition may be formulated to enable effective reaction mixture loading onto a dPCR plate, provide high proportion of valid/readable partitions, enable identification and distinguishing between amplified and unamplified partitions, enable detection of low abundance targets amidst high background levels of non-target nucleic acids and/or amidst inhibiting agents, and/or enable effective detection of low frequency mutant alleles.
A method of registering a location of a dispenser array in relation to a microfluidic array is provided. One of the dispenser array and the microfluidic array can be movable in relation to the frame, and the other can be fixed relative to the frame. Their relative positions can be identified by a set of coordinates. Identification of a fiducial marker can occur in a manner permitting the fiducial reference to appear in a first position of a field of view of a first camera when the dispenser array or the microfluidic array is in an alignment position. Quantities related to a vector displacement from the alignment position to a fixed position on the microfluidic array or the dispenser array can be identified. Quantities determined can be used to guide positioning of the dispenser array relative to the microfluidic array.
The instant technology relates to a production system to produce AAV vectors in a serum free suspension platform and at high titers. This technology uses reagents comprising media, cells, transfection reagent, AAV enhancer, and a lysis buffer, each of which is designed to provide maximal AAV production from suspension culture of mammalian cells, e.g. HEK293 cells. With this new system we are able to deliver up to about 2×1011 viral genomes per milliliter (vg/mL) of unconcentrated AAV vectors.
Purifying target biomolecules, such as nucleic acids or proteins, from a biological source is a time intensive process and is typically performed by a skilled technician or scientist owing to the highly technical nature of the work. Systems, devices, and methods disclosed herein enable the automated bioprocessing and purification of target biomolecules from a biological source. For example, an instrument and disposable cartridge are provided for automatedly isolating and purifying nucleic acids (such as plasmid DNA from a bacterial culture) or for isolating protein from any biological sample. Such an exemplary instrument and cartridge can work in concert to timely release, mix, and move the target biomolecule and various reagents and buffers through a target biomolecule purification process, resulting in a purified target biomolecule with less manual oversight than traditional approaches.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
B01D 69/02 - Membranes semi-perméables destinées aux procédés ou aux appareils de séparation, caractérisées par leur forme, leur structure ou leurs propriétésProcédés spécialement adaptés à leur fabrication caractérisées par leurs propriétés
G01N 35/00 - Analyse automatique non limitée à des procédés ou à des matériaux spécifiés dans un seul des groupes Manipulation de matériaux à cet effet
72.
METHODS, SYSTEMS, AND COMPUTER READABLE MEDIA FOR NUCLEIC ACID SEQUENCING
A method for nucleic acid sequencing includes receiving a plurality of signals indicative of a parameter measured for a plurality of defined spaces, at least some of the defined spaces including one or more sample nucleic acids, the signals being responsive to a plurality of nucleotide flows introducing nucleotides to the defined spaces; determining, for at least some of the defined spaces, whether the defined space includes a sample nucleic acid; processing, for at least some of the defined spaces determined to include a sample nucleic acid, the received signals to improve a quality of the received signals; and predicting a plurality of nucleotide sequences corresponding to respective sample nucleic acids for the defined spaces based on the processed signals and the nucleotide flows.
G01N 27/414 - Transistors à effet de champ sensibles aux ions ou chimiques, c.-à-d. ISFETS ou CHEMFETS
G01N 27/27 - Association de plusieurs systèmes ou cellules de mesure, chacun mesurant un paramètre différent, dans laquelle les résultats des mesures peuvent être, soit utilisès indépendamment, les systèmes ou les cellules étant physiquement associés, soit combinés pour produire une valeur représentative d'un autre paramètre
G16B 30/00 - TIC spécialement adaptées à l’analyse de séquences impliquant des nucléotides ou des aminoacides
G16B 30/10 - Alignement de séquenceRecherche d’homologie
The present disclosure relates to automated chromatography systems and methods for improving the accuracy of a variety of sensors in a chromatography system and measuring liquid and system parameters of a variety of liquids and bioprocessing equipment used to purify a target molecule. The automated chromatography systems and methods can run a startup operation to generate liquid and sensor parameter sets used in purification operations and recipes for purifying a target molecule in a chromatography column.
Disclosed are compositions, kits, and methods for quantifying a target nucleic acid from a sample. Compositions, kits, and methods enable the comparison of target nucleic acid loads between two or more test samples by normalizing measured levels (using a standard curve) of the target nucleic acid in each sample according to relative levels of endogenous nucleic acid in each test sample.
C12Q 1/70 - Procédés de mesure ou de test faisant intervenir des enzymes, des acides nucléiques ou des micro-organismesCompositions à cet effetProcédés pour préparer ces compositions faisant intervenir des virus ou des bactériophages
The present disclosure relates to systems and methods for efficiently and easily implementing user-friendly purification operations and recipes to purify a target molecule by column chromatography. More specifically, the present application and disclosure relate to chromatography instrument control system modules, libraries and user interfaces used to control a chromatography instrument. The systems and methods are configured to provide a user with the ability to create, modify, recall, store, organize, prioritize, and run complex purification recipes to control a piece of chromatography equipment through a system controller and one or more graphical user interfaces.
A method for mixing a fluid includes: dispensing a first volume of a fluid into a flexible container, the flexible container being at least partially disposed within the chamber of a support housing; repeatedly moving the support housing and the flexible container contained therein so as to mix the first volume of fluid within the flexible container; adding further fluid into the flexible container after moving the support housing to form a second volume of fluid; and manipulating a mixing element within the flexible container so as to mix the second volume of fluid.
B01F 35/513 - Récipients souples, p. ex. sacs supportés par des conteneurs rigides
B01F 27/07 - Agitateurs caractérisés par leur montage sur l’arbre
B01F 27/213 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins caractérisés par leurs arbres de rotation caractérisés par la liaison avec l'entraînement
B01F 27/807 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins avec des agitateurs tournant autour d'un axe sensiblement vertical les agitateurs ou les récipients étant déplacés afin de les mettre en position de fonctionnementMoyens de fixation du récipient avec la tête d'agitation pivotant autour d'un axe horizontal pour l'amener en et hors position de fonctionnement, p. ex. avec des récipients pivotant autour d'un axe horizontal pour la vidange
B01F 27/88 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins avec des agitateurs tournant autour d'un axe sensiblement vertical avec une unité récipient-agitateur séparée qui est adaptée pour être couplée à un mécanisme d'entraînement
B01F 27/91 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins avec des agitateurs tournant autour d'un axe sensiblement vertical avec des hélices
B01F 31/20 - Mélange du contenu de récipients indépendants, p. ex. de tubes à essai
B01F 31/23 - Mélange du contenu de récipients indépendants, p. ex. de tubes à essai en faisant pivoter les récipients autour d'un axe
B01F 35/42 - Dispositions de serrage ou de maintien pour le montage des récipients sur les dispositifs de mélange
B01F 35/43 - Récipients de soutien sur des cadres ou des supports
C12M 1/00 - Appareillage pour l'enzymologie ou la microbiologie
C12M 1/02 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'agitationAppareillage pour l'enzymologie ou la microbiologie avec des moyens d'échange de chaleur
C12M 1/06 - Appareillage pour l'enzymologie ou la microbiologie avec des moyens d'introduction de gaz avec agitateur, p. ex. avec agitateur à turbine
C12M 3/00 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus
C12M 3/06 - Appareillage pour la culture de tissus, de cellules humaines, animales ou végétales, ou de virus avec des moyens de filtration, d'ultrafiltration, d'osmose inverse ou de dialyse
77.
QUALITY CONTROL AUTOMATION IN POLYMERASE CHAIN REACTION ANALYSIS
Methods and systems are disclosed for computerized image processing for digital polymerase chain reaction (dPCR) analysis of a biological sample to automatically reject data of individual partitions from use in computing a target concentration result. Image data representing a plurality of partitions disposed in a container is obtained, including background image data captured prior to amplification cycles of a dPCR assay, and endpoint image data captured after amplification. Individual partition image data for the plurality of partitions is extracted from the image data. The partition background image data and the partition endpoint image data are pre-processed to obtain processed image data for input into one or more machine learning models, which are used to generate classification results. The one or more classification results are used to determine whether to reject image data corresponding to the partition of the plurality of partitions from use in computing the target concentration result.
Embodiments of a digital polymerase chain reaction (dPCR) system and method having improved auto-thresholding performance and accuracy are disclosed. One embodiment of the disclosure comprises a dPCR processing module including an auto-thresholding agent that dynamically analyzes and selects between Gaussian Mixture Modeling (GMM) and K-means clustering for use in auto-thresholding results of a dPCR assay using a dPCR instrument to improve dPCR measurement technology.
Various embodiments for improved dPCR systems and methods are disclosed. Some embodiments provide a multi-instrument dPCR system that facilitates analyzing samples together across multiple arrays of partitions, multiple sample plates, and/or multiple instruments to provide a better and higher throughput dPCR system. Aspects of various embodiments provide one or more of the following: Correction of illumination bias; quality control processing; flexible grouping of samples across partitions, sample plates, and instruments for thresholding and analysis; inter-instrument signal equalization for each dye channel to facilitate grouping samples across instruments; and improved auto-thresholding.
The present disclosure relates to automated chromatography systems and methods for improving the accuracy of a variety of sensors in a chromatography system and measuring liquid and system parameters of a variety of liquids and bioprocessing equipment used to purify a target molecule. The automated chromatography systems and methods can run a startup operation to generate liquid and sensor parameter sets used in purification operations and recipes for purifying a target molecule in a chromatography column.
Disclosed embodiments include a digital Polymerase chain reaction (dPCR) operative system having a method of digital image processing that corrects for illumination bias in data relating to an array of a sample plate comprising one or more arrays of sample partitions. In some embodiments, the method includes obtaining background image data corresponding to a digital image captured by the camera of the dPCR system after a background cycle of a dPCR assay. With respect to each partition, a local flat-fielding coefficient is derived and applied to the background image data to obtain local-bias-corrected background image data. Next, the local‑bias‑corrected background data is used to determine a set of global flat-fielding coefficients. The local and global flat-fielding coefficients are subsequently used to correct illumination bias endpoint image data to generate endpoint bias-corrected illumination values.
A computer-implemented method for visualizing dye interaction in a multiplexed biological sample is provided. The method includes receiving fluorescent emission data from each reaction site of a plurality of reaction sites. The reaction sites include at least a first, second, and third dye. The method further includes determining intensity values for at least a first, second, and third dye channel from the fluorescent emission data from each reaction site. The method further includes displaying, on a user interface, a first set of indications of a plurality of intensity values of the fluorescent emission data for the first, second, and third dye detected in the first dye channel, detected in the second dye channel, and detected in the third dye channel. The method further includes adjusting a label of the fluorescent emission data from a reaction site by comparing the first set of indications.
Apparatus and instruments for clinical and medical
diagnosis, namely, nucleic acid sequencers and synthesizers,
genetic analyzers, and instruments for the preparation of
nucleic acid samples.
A system for sample imaging includes a control unit for delivering conditioned air, and a specimen chamber that receives the conditioned air from the control unit. The specimen chamber includes a chamber housing having an upper face, a lower face opposite the upper face and configured to face an imaging lens, and walls that extend vertically between the upper and lower faces. The walls define an interior volume of the specimen chamber. The specimen chamber includes an air actuator unit configured to direct conditioned air to a target location alongside the lower face for inhibiting or at least reducing condensation accumulation on the imaging lens.
The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.
B01L 3/00 - Récipients ou ustensiles pour laboratoires, p. ex. verrerie de laboratoireCompte-gouttes
G01J 3/44 - Spectrométrie RamanSpectrométrie par diffusion
G01N 15/02 - Recherche de la dimension ou de la distribution des dimensions des particules
G01N 15/0227 - Recherche de la dimension ou de la distribution des dimensions des particules par des moyens optiques utilisant l’imagerieRecherche de la dimension ou de la distribution des dimensions des particules par des moyens optiques utilisant l’holographie
G01N 15/14 - Techniques de recherche optique, p. ex. cytométrie en flux
G01N 15/1404 - Manipulation du flux, p. ex. focalisation hydrodynamique
G01N 15/00 - Recherche de caractéristiques de particulesRecherche de la perméabilité, du volume des pores ou de l'aire superficielle effective de matériaux poreux
G01N 15/10 - Recherche de particules individuelles
G01N 15/149 - Techniques de recherche optique, p. ex. cytométrie en flux spécialement adaptées au tri des particules, p. ex. selon leur taille ou leurs propriétés
The present disclosure relates to a mixing assembly for a fluid mixing system. The mixing assembly includes a first rotational assembly having a first casing and a first hub rotatably mounted to the first casing, a second rotational assembly having a second casing and a second hub rotatably mounted to the second casing, an elongated member having a first end coupled with the first hub and an opposing second end coupled with the second hub, and one or more impellers secured to the elongated member.
B01F 27/2121 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins caractérisés par leurs arbres de rotation composés de parties interconnectées
B01F 27/191 - Agitateurs avec plusieurs éléments de mélange montés en séquence sur un même axe avec des éléments similaires
B01F 27/213 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins caractérisés par leurs arbres de rotation caractérisés par la liaison avec l'entraînement
B01F 27/80 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins avec des agitateurs tournant autour d'un axe sensiblement vertical
B01F 27/88 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins avec des agitateurs tournant autour d'un axe sensiblement vertical avec une unité récipient-agitateur séparée qui est adaptée pour être couplée à un mécanisme d'entraînement
B01F 27/91 - Mélangeurs à agitateurs tournant dans des récipients fixesPétrins avec des agitateurs tournant autour d'un axe sensiblement vertical avec des hélices
B01F 33/00 - Autres mélangeursInstallations pour effectuer des mélangesCombinaisons de mélangeurs
A thermal cycler system comprises a sample block configured to receive a sample holder configured to receive a plurality of samples; an adaptor configured to surround a periphery of the sample block; and a drip pan configured to surround a periphery of the adaptor. The drip pan comprises one or more ejector mechanisms and one or more openings, the one or more ejector mechanisms configured to respectively extend through the one or more openings into contact with the sample holder in a state of the sample holder received by the sample block and the adaptor surrounding the periphery of the sample block.
The invention provides a passive fluidics circuit for directing different fluids to a common volume, such as a reaction chamber or flow cell, without intermixing or cross contamination. The direction and rate of flow through junctions, nodes and passages of the fluidics circuit are controlled by the states of upstream valves (e.g. opened or closed), differential fluid pressures at circuit inlets or upstream reservoirs, flow path resistances, and the like. Free diffusion or leakage of fluids from unselected inlets into the common outlet or other inlets at junctions or nodes is prevented by the flow of the selected inlet fluid, a portion of which sweeps by the inlets of unselected fluids and exits the fluidics circuit by waste ports, thereby creating a barrier against undesired intermixing with the outlet flow through leakage or diffusion.
G01N 35/10 - Dispositifs pour transférer les échantillons vers, dans ou à partir de l'appareil d'analyse, p. ex. dispositifs d'aspiration, dispositifs d'injection
92.
METHODS FOR SENSOR DEVICE SAMPLE INCUBATION LOADING
For various sequencing systems, the effective loading of template beads into a sensor device is an essential step in a number of essential steps in providing high quality sequencing data. The loading of various types of template beads into a microwell array of a sensor device can be effectively accomplished via incubation loading. The time required for incubation loading of a sensor device can be well within a desired device loading workflow for sequencing of between about ten minutes to about an hour.
Disclosed are primer set compositions, methods and kits for human identification using the highly complex sequence locus, SE33 (ACTBP2) in single and multiplex PCR reactions. Additionally, disclosed are three newly discovered single nucleotide polymorphisms (SNPs) within the SE33 locus that can cause discordance seen as mobility shift or allelic dropout. Also disclosed are kits useful in human identification.
C12Q 1/6827 - Tests d’hybridation pour la détection de mutation ou de polymorphisme
C12Q 1/686 - Réaction en chaine par polymérase [PCR]
C12Q 1/6888 - Produits d’acides nucléiques utilisés dans l’analyse d’acides nucléiques, p. ex. amorces ou sondes pour la détection ou l’identification d’organismes
98.
ROOM-TEMPERATURE-STABLE CULTURE MEDIA AND SUPPLEMENTS
Described herein are formulations of cell culture media and supplements that are stable when stored at room temperature. Embodiments described herein encompass dry granulated or agglomerated cell culture media with carbohydrate sources and vitamins that are physically separated. Further embodiments described herein are dry granulated or agglomerated cell culture media with a carbohydrate source that is separately agglomerated or powdered. Additional embodiments are dry granulated or agglomerated cell culture media with a carbohydrate source that is separately agglomerated or powdered and optionally contains vitamins. In another embodiment, the culture media or supplement is a dry granulated or agglomerated cell culture media containing granulated or agglomerated or powdered carbohydrates and vitamins in one or more of a liquid-, powdered-, granulated-, agglomerated-, coated-, or encapsulated-form.
C12N 5/00 - Cellules non différenciées humaines, animales ou végétales, p. ex. lignées cellulairesTissusLeur culture ou conservationMilieux de culture à cet effet
C12N 5/073 - Cellules ou tissus embryonnairesCellules fœtales ou tissus fœtaux
05 - Produits pharmaceutiques, vétérinaires et hygièniques
Produits et services
Assays, reagents, enzymes, polymerases, buffers and
nucleotides, and kits comprised thereof, all for medical or
diagnostic use (Term considered too vague by the
International Bureau pursuant to Rule 13 (2) (b) of the
Regulations).
100.
Device Preparation Using Condensed Nucleic Acid Particles
A method for depositing particles on a surface includes receiving a plurality of particles, a particle of the plurality of particles having a polymer matrix conjugated to nucleic acid strands, exposing the plurality of particles to a solution; and applying the plurality of particles to a surface following exposing the plurality of particles to the solution, particles of the plurality of particles depositing on the surface. The solution includes a magnesium salt in a range of 30 mM to 500 mM and polyethylene glycol.
